RLG00000028228

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005289
Physical Location & Seq
Reverse (-)
20327092 .. 20330005
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028228

Sequence Viewer

Length: 1302 bp
ATGACGTCGCCTCCCTGCTTGGAAACGGCGTCGTTTTTTCCTTCTTCTACAGACTCAGATTGGGGACACAAAGTCTCAGACAAGGTGCTCTCAGTCTACAACTCGCTCCCCAAGAAAGGCAAGCCTCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGAATTGGAAGTTGTAGCATTGGGAACCGGAACCAAATGCGTCGGCCGTTCACTCCTCAGCCCCCACGGCGATGTCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGGTTCTTCTATGCACAGATACAATGTGTTTCTCAAATTTATACTAGGCAAAGAGATAACAATGGCAGCAAACAGTTGCGAAACGATGATGCGAAAAAGTTTATTTTTGAATTGGACCCTGATGATGGGCAAGGAAAATACACAATGAGGAAGGGTTGGAAATTGCACATGTATATATCGCAACTACCTTGTGGGATTGCTTCGCCAAGTTCATTGATGTCTTCCCCGAAAAATATCTCACCTAGCGGAGGGGGTTCATTAGTGGAGGAGCTTAATGAGTCCATAAATAAGGAGGCTTTGATTGAGAATAATGGTGACGCTTCACAGCTAATTGGCACGGTTCAAAGAAAGCCTGGTCGTGGTGATACAACATTATCAGTTAGCTGCTCTGACAAGATAGCCCGTTGGAATGTTGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCCGAAATGGAAATTGTGGTTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCAAGCGAGCTGACCAGTCCCTTTCAGGTGAACCAGCCGCTACTCTGGGAAGCTCCAATGCCACCAAAGGAATTTCAACATTCTGAGACTGCTTTGACCACTATAACATGCGGGTATTCAATATGTTGGAATAAGTCTGGTTTGCATGAAGTCATTCTTGGAACCATAGGAAGAAAGCAAGGAGCTGCAAAAGGAGCACGCAGTCCGTCTACTGAATCCCCTATATGCAAAAAACGGCTCTTGGAGATTTTCTTGTCCCTAAGGAATGAATGCCCCACCAATATCCATGTCAATGATATTTCGTATCGAGAAATTAAGGAAAGGGCTTATAAATACAATGCAATATTTGGAGGCATTCTCTGCTATGGGAAAGTGAGTTTCAGGACACCGATGTGGCTTAAAAGGGTTTCTAGCAGCTTTGAGGATAAGGATATCATATGA

Protein Analysis

434

Amino Acids

47.78

Weight (kDa)

8.65

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 66 - 401 5.5e-78 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1191
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 3 cut(s) 96, 759, 1070
AccII CGCG 2 cut(s) 135, 291
AciI CCGC 6 cut(s) 133, 153, 289, 546, 899, 972
AcoI YGGCCR 2 cut(s) 150, 223
AcsI RAATTY 2 cut(s) 336, 932
AcyI GRCGYC 2 cut(s) 5, 29
AfiI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 7 cut(s) 179, 410, 644, 725, 752, 938, 981
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AleI CACNNNNGTG 1 cut(s) 1252
AluBI AGCT 9 cut(s) 571, 628, 684, 731, 836, 871, 914, 1046, 1278
AluI AGCT 9 cut(s) 571, 628, 684, 731, 836, 871, 914, 1046, 1278
Alw21I GWGCWC 2 cut(s) 90, 1060
Alw26I GTCTC 2 cut(s) 79, 941
AoxI GGCC 3 cut(s) 130, 150, 223
ApeKI GCWGC 4 cut(s) 366, 684, 1046, 1275
ApoI RAATTY 2 cut(s) 336, 932
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
Asp700I GAANNNNTTC 1 cut(s) 1014
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 130, 415
AsuHPI GGTGA 5 cut(s) 133, 531, 626, 674, 901
AvaII GGWCC 1 cut(s) 415
AxyI CCTNAGG 2 cut(s) 126, 1121
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 90, 1060
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 4 cut(s) 378, 671, 1033, 1287
BccI CCATC 1 cut(s) 419
BceAI ACGGC 4 cut(s) 42, 210, 262, 1112
BciT130I CCWGG 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 79, 941
BfaI CTAG 3 cut(s) 345, 543, 1272
BfmI CTRYAG 1 cut(s) 48
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 8 cut(s) 133, 153, 289, 367, 685, 899, 1047, 1276
BlsI GCNGC 8 cut(s) 134, 154, 290, 368, 686, 900, 1048, 1277
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 1 cut(s) 415
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 130, 415
BmiI GGNNCC 4 cut(s) 205, 211, 417, 1024
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 1 cut(s) 379
BpiI GAAGAC 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 1203, 1235
Bpu10I CCTNAGC 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 5, 29
BsaJI CCNNGG 2 cut(s) 244, 273
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
Bse1I ACTGG 1 cut(s) 876
Bse21I CCTNAGG 2 cut(s) 126, 1121
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 2 cut(s) 244, 273
BseLI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
BseMII CTCAG 6 cut(s) 69, 90, 105, 140, 250, 936
BseNI ACTGG 1 cut(s) 876
BseRI GAGGAG 5 cut(s) 152, 157, 162, 224, 581
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 4 cut(s) 378, 671, 1033, 1287
Bsh1236I CGCG 2 cut(s) 135, 291
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 132, 152, 225
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 90, 1060
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 3 cut(s) 78, 864, 1102
BslI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 548, 659
BsmAI GTCTC 2 cut(s) 79, 941
BsmFI GGGAC 3 cut(s) 78, 864, 1102
BsmI GAATGC 2 cut(s) 1136, 1215
BsnI GGCC 3 cut(s) 132, 152, 225
Bsp1286I GDGCHC 2 cut(s) 90, 1060
Bsp143I GATC 1 cut(s) 844
BspACI CCGC 6 cut(s) 133, 153, 289, 546, 899, 972
BspANI GGCC 3 cut(s) 132, 152, 225
BspCNI CTCAG 6 cut(s) 68, 89, 104, 139, 249, 937
BspFNI CGCG 2 cut(s) 135, 291
BspLI GGNNCC 4 cut(s) 205, 211, 417, 1024
BsrI ACTGG 1 cut(s) 876
BssECI CCNNGG 2 cut(s) 244, 273
BssMI GATC 1 cut(s) 844
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 2 cut(s) 5, 29
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 4 cut(s) 145, 375, 640, 778
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 2 cut(s) 5, 29
BstAPI GCANNNNNTGC 1 cut(s) 1221
BstC8I GCNNGC 4 cut(s) 122, 286, 869, 1060
BstDEI CTNAG 7 cut(s) 55, 76, 91, 126, 236, 945, 1121
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 546
BstFNI CGCG 2 cut(s) 135, 291
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 2 cut(s) 79, 941
BstMBI GATC 1 cut(s) 844
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 246, 290, 1055, 1221
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 2 cut(s) 472, 972
BstSCI CCNGG 1 cut(s) 652
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 2 cut(s) 135, 291
BstV1I GCAGC 4 cut(s) 378, 671, 1033, 1287
BstV2I GAAGAC 1 cut(s) 513
BstZ17I GTATAC 1 cut(s) 760
BstZI CGGCCG 1 cut(s) 223
Bsu36I CCTNAGG 2 cut(s) 126, 1121
BsuRI GGCC 3 cut(s) 132, 152, 225
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 2 cut(s) 264, 265
Cac8I GCNNGC 4 cut(s) 122, 286, 869, 1060
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 130, 415
CseI GACGC 3 cut(s) 18, 208, 626
CviAII CATG 5 cut(s) 469, 842, 969, 1007, 1148
DdeI CTNAG 7 cut(s) 55, 76, 91, 126, 236, 945, 1121
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 223
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 223
Eco81I CCTNAGG 2 cut(s) 126, 1121
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 1294
FaeI CATG 5 cut(s) 472, 845, 972, 1010, 1151
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 3 cut(s) 78, 864, 1102
FatI CATG 5 cut(s) 468, 841, 968, 1006, 1147
FauI CCCGC 1 cut(s) 965
FauNDI CATATG 1 cut(s) 1298
FblI GTMKAC 3 cut(s) 96, 759, 1070
Fnu4HI GCNGC 8 cut(s) 133, 153, 289, 367, 685, 899, 1047, 1276
Fsp4HI GCNGC 8 cut(s) 133, 153, 289, 367, 685, 899, 1047, 1276
FspBI CTAG 3 cut(s) 345, 543, 1272
GlaI GCGC 1 cut(s) 292
GluI GCNGC 8 cut(s) 133, 153, 289, 367, 685, 899, 1047, 1276
HaeIII GGCC 3 cut(s) 132, 152, 225
HapII CCGG 1 cut(s) 207
HgaI GACGC 3 cut(s) 18, 208, 626
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 2 cut(s) 5, 29
Hin1II CATG 5 cut(s) 472, 845, 972, 1010, 1151
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 53, 263, 578, 1076
HpaII CCGG 1 cut(s) 207
HphI GGTGA 5 cut(s) 133, 531, 626, 674, 901
Hpy166II GTNNAC 6 cut(s) 97, 230, 259, 760, 892, 1071
Hpy188I TCNGA 6 cut(s) 58, 79, 691, 802, 828, 946
Hpy188III TCNNGA 3 cut(s) 179, 1169, 1243
Hpy8I GTNNAC 6 cut(s) 97, 230, 259, 760, 892, 1071
Hpy99I CGWCG 3 cut(s) 10, 34, 224
HpyAV CCTTC 4 cut(s) 51, 166, 445, 751
HpyCH4III ACNGT 4 cut(s) 145, 375, 640, 778
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 7 cut(s) 314, 466, 841, 1006, 1049, 1089, 1202
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 290, 1055, 1221
HpyF3I CTNAG 7 cut(s) 55, 76, 91, 126, 236, 945, 1121
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 2 cut(s) 5, 29
Hsp92II CATG 5 cut(s) 472, 845, 972, 1010, 1151
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 1 cut(s) 844
LmnI GCTCC 6 cut(s) 111, 568, 728, 919, 1043, 1055
Lsp1109I GCAGC 4 cut(s) 378, 671, 1033, 1287
LweI GCATC 1 cut(s) 379
MaeI CTAG 3 cut(s) 345, 543, 1272
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 139, 614
MalI GATC 1 cut(s) 846
MboI GATC 1 cut(s) 844
MboII GAAGA 7 cut(s) 36, 167, 298, 513, 740, 759, 1044
MhlI GDGCHC 2 cut(s) 90, 1060
MluCI AATT 8 cut(s) 182, 336, 410, 461, 630, 810, 932, 1173
MlyI GAGTC 2 cut(s) 47, 587
MmeI TCCRAC 5 cut(s) 437, 686, 697, 760, 968
MroXI GAANNNNTTC 1 cut(s) 1014
MseI TTAA 4 cut(s) 296, 573, 1176, 1260
MslI CAYNNNNRTG 4 cut(s) 249, 846, 1152, 1252
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 1136, 1215
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 2 cut(s) 135, 291
MwoI GCNNNNNNNGC 4 cut(s) 246, 290, 1055, 1221
NdeI CATATG 1 cut(s) 1298
NdeII GATC 1 cut(s) 844
NlaIII CATG 5 cut(s) 472, 845, 972, 1010, 1151
NlaIV GGNNCC 4 cut(s) 205, 211, 417, 1024
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 139, 614
NspI RCATGY 2 cut(s) 472, 972
OliI CACNNNNGTG 1 cut(s) 1252
PciI ACATGT 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 2 cut(s) 1136, 1215
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 2 cut(s) 263, 1076
PkrI GCNGC 8 cut(s) 134, 154, 290, 368, 686, 900, 1048, 1277
PleI GAGTC 2 cut(s) 47, 586
PpsI GAGTC 2 cut(s) 47, 586
PscI ACATGT 1 cut(s) 468
PsiI TTATAA 1 cut(s) 1191
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 4 cut(s) 205, 211, 417, 1024
PspPI GGNCC 2 cut(s) 130, 415
RseI CAYNNNNRTG 4 cut(s) 249, 846, 1152, 1252
SaqAI TTAA 4 cut(s) 296, 573, 1176, 1260
SatI GCNGC 8 cut(s) 133, 153, 289, 367, 685, 899, 1047, 1276
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 2 cut(s) 130, 415
SchI GAGTC 2 cut(s) 47, 587
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 2 cut(s) 90, 1060
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 4 cut(s) 249, 846, 1152, 1252
Sse9I AATT 8 cut(s) 182, 336, 410, 461, 630, 810, 932, 1173
SsiI CCGC 6 cut(s) 133, 153, 289, 546, 899, 972
SspI AATATT 1 cut(s) 1206
SspMI CTAG 3 cut(s) 345, 543, 1272
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 4 cut(s) 145, 375, 640, 778
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 1168
TasI AATT 8 cut(s) 182, 336, 410, 461, 630, 810, 932, 1173
TauI GCSGC 4 cut(s) 135, 155, 291, 901
TfiI GAWTC 2 cut(s) 263, 1076
Tru1I TTAA 4 cut(s) 296, 573, 1176, 1260
Tru9I TTAA 4 cut(s) 296, 573, 1176, 1260
TseFI GTSAC 2 cut(s) 139, 614
TseI GCWGC 4 cut(s) 366, 684, 1046, 1275
Tsp45I GTSAC 2 cut(s) 139, 614
TspDTI ATGAA 4 cut(s) 501, 546, 1023, 1143
TspGWI ACGGA 1 cut(s) 1056
VpaK11BI GGWCC 1 cut(s) 415
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 2 cut(s) 336, 932
XceI RCATGY 2 cut(s) 472, 972
XmiI GTMKAC 3 cut(s) 96, 759, 1070
XmnI GAANNNNTTC 1 cut(s) 1014
XspI CTAG 3 cut(s) 345, 543, 1272
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.