Rorug01G0241100

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005289
Physical Location & Seq
Forward (+)
34611048 .. 34611542
495 bp
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UTR
Exon/CDS
Intron
Rorug01G0241100.1

Sequence Viewer

Length: 495 bp
ATGTTCCTGGCTTATTCAAGAAATTTTGATGTTGTTCAAGCTCACTCTCAAAAAGCTCATTCTCCTAATACTAACATTATTAAGTGGCTTCCTCCTCCCTCTAATTTTGTTAAACTCAACTTTGATGGTTCTGTGCGACATAACAAACAAGGTGCTGCAGGTTTTATTATCTGGAATGATCAGGGTATTCCTATCCTAGCCTCTTCTAAGAGACTAGGTCATTCTAATGTCCTTGTAGCTGAAGCTATGGCTCTTAGAGCAGGTCTACAGGCTGTTGTTCTTCATGGTATGTCAAAAATTAAAGTTGAAGGCGATTCAAAGACCTTGATCGACTGTATTAACAATAAGATCACAGTTCCTCGGAAGATTCATATGCTAGTTTTGATATCAAAATGTTGGCTTCTCATTTCTCGGACATTGAATTCAAGCACGTTTGTCATGAGGCGAACTTTGTTGCAGATGCTCTTGCGGATTTGGGTCACGACACAGACATAG

Protein Analysis

164

Amino Acids

18.4

Weight (kDa)

10.62

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 40 - 139 3.2e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 149, 251
AccI GTMKAC 1 cut(s) 265
AciI CCGC 1 cut(s) 469
AcsI RAATTY 2 cut(s) 22, 421
AcuI CTGAAG 1 cut(s) 261
AgsI TTSAA 6 cut(s) 18, 38, 308, 318, 421, 426
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 4 cut(s) 41, 56, 239, 245
AluI AGCT 4 cut(s) 41, 56, 239, 245
Alw26I GTCTC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 2 cut(s) 22, 421
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 8
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 3 cut(s) 197, 215, 377
BfmI CTRYAG 2 cut(s) 156, 266
BfuAI ACCTGC 2 cut(s) 149, 251
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 8
BmrFI CCNGG 1 cut(s) 8
BmsI GCATC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 359
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 359
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 205
Bsp143I GATC 3 cut(s) 178, 327, 348
BspACI CCGC 1 cut(s) 469
BspHI TCATGA 1 cut(s) 438
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 2 cut(s) 149, 251
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 3 cut(s) 178, 327, 348
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 2 cut(s) 335, 355
Bst6I CTCTTC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 207, 254
BstKTI GATC 3 cut(s) 181, 330, 351
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 3 cut(s) 178, 327, 348
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstSFI CTRYAG 2 cut(s) 156, 266
BstV1I GCAGC 1 cut(s) 142
BveI ACCTGC 2 cut(s) 149, 251
CciI TCATGA 1 cut(s) 438
CviAII CATG 2 cut(s) 284, 439
DdeI CTNAG 2 cut(s) 207, 254
DpnI GATC 3 cut(s) 180, 329, 350
DpnII GATC 3 cut(s) 178, 327, 348
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco32I GATATC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 6
EcoRV GATATC 1 cut(s) 387
FaeI CATG 2 cut(s) 287, 442
FaiI YATR 8 cut(s) 141, 248, 285, 290, 372, 374, 440, 493
FatI CATG 2 cut(s) 283, 438
FauNDI CATATG 1 cut(s) 372
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 156
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 3 cut(s) 197, 215, 377
GluI GCNGC 1 cut(s) 156
Hin1II CATG 2 cut(s) 287, 442
HinfI GANTC 2 cut(s) 314, 367
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 2 cut(s) 363, 414
Hpy188III TCNNGA 4 cut(s) 18, 172, 439, 481
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 2 cut(s) 335, 355
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 2 cut(s) 158, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 2 cut(s) 207, 254
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 2 cut(s) 287, 442
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 3 cut(s) 178, 327, 348
LpnPI CCDG 6 cut(s) 20, 144, 157, 167, 246, 254
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 450
MaeI CTAG 3 cut(s) 197, 215, 377
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 180, 329, 350
MboI GATC 3 cut(s) 178, 327, 348
MboII GAAGA 3 cut(s) 195, 272, 376
MluCI AATT 4 cut(s) 22, 103, 297, 421
MnlI CCTC 6 cut(s) 102, 105, 109, 211, 369, 435
MseI TTAA 4 cut(s) 81, 111, 300, 339
MslI CAYNNNNRTG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 257
NdeI CATATG 1 cut(s) 372
NdeII GATC 3 cut(s) 178, 327, 348
NlaIII CATG 2 cut(s) 287, 442
NmuCI GTSAC 1 cut(s) 478
PagI TCATGA 1 cut(s) 438
PfeI GAWTC 2 cut(s) 314, 367
PflFI GACNNNGTC 1 cut(s) 216
PkrI GCNGC 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PstI CTGCAG 1 cut(s) 160
PsyI GACNNNGTC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 4 cut(s) 81, 111, 300, 339
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 3 cut(s) 178, 327, 348
ScrFI CCNGG 1 cut(s) 8
SfaNI GCATC 1 cut(s) 450
SfcI CTRYAG 2 cut(s) 156, 266
SmiMI CAYNNNNRTG 1 cut(s) 225
Sse9I AATT 4 cut(s) 22, 103, 297, 421
SsiI CCGC 1 cut(s) 469
SspMI CTAG 3 cut(s) 197, 215, 377
StyD4I CCNGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 335, 355
TaiI ACGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 330
TasI AATT 4 cut(s) 22, 103, 297, 421
TfiI GAWTC 2 cut(s) 314, 367
Tru1I TTAA 4 cut(s) 81, 111, 300, 339
Tru9I TTAA 4 cut(s) 81, 111, 300, 339
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 272, 359
Tth111I GACNNNGTC 1 cut(s) 216
XapI RAATTY 2 cut(s) 22, 421
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 3 cut(s) 197, 215, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.