Rh1CG236400

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005289
Physical Location & Seq
Forward (+)
49403758 .. 49405358
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG236400.1

Sequence Viewer

Length: 921 bp
ATGACGTCGCCTCCCTGCTTCGAAACGCCGTCGTTTCGTCCTTCTTCTCCAGACTCAGACTGGGGACACAAAGTCTCAGACAAGGTGCTCTCAGTCTACAACTCGCTCCCTAAGAAAGGGAAGCCCCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGAATTGGAAGTTGTGGCATTGGGAACCGGAACCAAATGCGTCGGCCGTTCACTCCTCAGCCCCCACGGCGATATCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGGTTCTTCTATGCACAGATACAATGTCTTTCTCAAATTTATAGTAAGCAAAGAGATAACAATGGAAGCAAACAGTTGCGAAACGATGATGCGAAAAAGTTTATTTTTGAATTGGACCCTGATGATGGGCAAGGAAAATACACAATGAGGAAGGGTTGGAAATTGCACATGTATATATCGCAACTACCTTGTGGGATTGCTTCGCCAAGTTCATTGATGTCTCCCCTGAAAAATATCTCACCTAGTGGAGTGGGTTCATTACTGGAGGAGCTTAATGAGTCCATAAATGAGGAGGCTTTGATTGAGAATAATGGTGACGCTTCACAGCTAATTGGCACAGTTCAAAGAAAGCCTGGTCGTGGTGATACAACATTATCAGTTAGCTGCTCTGACAAGATAGCCCGTTGGAATGTTGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCCGAAAAGGAACATGTGGTTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCGAGCGAGCTGACCAGTCCCTTTCAGATGAACCAGGTCTGTGAGGTGTCTACTCTGTGA

Protein Analysis

306

Amino Acids

33.52

Weight (kDa)

6.6

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 66 - 301 3e-59 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 3 cut(s) 96, 759, 911
AccII CGCG 2 cut(s) 135, 291
AciI CCGC 3 cut(s) 133, 153, 289
AcoI YGGCCR 2 cut(s) 150, 223
AcsI RAATTY 1 cut(s) 336
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 545
AfiI CCNNNNNNNGG 4 cut(s) 116, 274, 425, 659
AflIII ACRYGT 2 cut(s) 468, 811
AgsI TTSAA 5 cut(s) 179, 410, 644, 725, 752
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 3 cut(s) 126, 652, 894
AluBI AGCT 6 cut(s) 571, 628, 684, 731, 836, 871
AluI AGCT 6 cut(s) 571, 628, 684, 731, 836, 871
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 79, 525
AoxI GGCC 3 cut(s) 130, 150, 223
ApeKI GCWGC 1 cut(s) 684
ApoI RAATTY 1 cut(s) 336
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 130, 415
AsuHPI GGTGA 4 cut(s) 133, 531, 626, 674
AsuII TTCGAA 1 cut(s) 21
AvaII GGWCC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 1 cut(s) 671
BccI CCATC 1 cut(s) 419
BceAI ACGGC 3 cut(s) 13, 210, 262
BciT130I CCWGG 3 cut(s) 128, 654, 896
BcoDI GTCTC 2 cut(s) 79, 525
BfaI CTAG 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 4 cut(s) 133, 153, 289, 685
BlsI GCNGC 4 cut(s) 134, 154, 290, 686
Bme1390I CCNGG 3 cut(s) 128, 654, 896
Bme18I GGWCC 1 cut(s) 415
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 130, 415
BmiI GGNNCC 3 cut(s) 205, 211, 417
BmrFI CCNGG 3 cut(s) 128, 654, 896
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 70
BpmI CTGGAG 2 cut(s) 33, 584
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 126, 127, 244, 273
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 274, 425, 659
Bse1I ACTGG 3 cut(s) 65, 567, 876
BseBI CCWGG 3 cut(s) 128, 654, 896
BseDI CCNNGG 4 cut(s) 126, 127, 244, 273
BseLI CCNNNNNNNGG 4 cut(s) 116, 274, 425, 659
BseMII CTCAG 4 cut(s) 69, 90, 105, 250
BseNI ACTGG 3 cut(s) 65, 567, 876
BseRI GAGGAG 6 cut(s) 152, 157, 162, 224, 581, 605
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 671
Bsh1236I CGCG 2 cut(s) 135, 291
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 132, 152, 225
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 78, 864
BslI CCNNNNNNNGG 4 cut(s) 116, 274, 425, 659
BsmAI GTCTC 2 cut(s) 79, 525
BsmFI GGGAC 2 cut(s) 78, 864
BsnI GGCC 3 cut(s) 132, 152, 225
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 1 cut(s) 844
BspACI CCGC 3 cut(s) 133, 153, 289
BspANI GGCC 3 cut(s) 132, 152, 225
BspCNI CTCAG 4 cut(s) 68, 89, 104, 249
BspFNI CGCG 2 cut(s) 135, 291
BspLI GGNNCC 3 cut(s) 205, 211, 417
BspT104I TTCGAA 1 cut(s) 21
BsrI ACTGG 3 cut(s) 65, 567, 876
BssECI CCNNGG 4 cut(s) 126, 127, 244, 273
BssMI GATC 1 cut(s) 844
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 3 cut(s) 128, 654, 896
Bst4CI ACNGT 4 cut(s) 145, 375, 640, 778
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 286, 869
BstDEI CTNAG 5 cut(s) 55, 76, 91, 111, 236
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 135, 291
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 2 cut(s) 79, 525
BstMBI GATC 1 cut(s) 844
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 246, 290
BstNI CCWGG 3 cut(s) 128, 654, 896
BstNSI RCATGY 2 cut(s) 472, 815
BstSCI CCNGG 3 cut(s) 126, 652, 894
BstUI CGCG 2 cut(s) 135, 291
BstV1I GCAGC 1 cut(s) 671
BstZ17I GTATAC 1 cut(s) 760
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 3 cut(s) 132, 152, 225
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 286, 869
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 130, 415
CseI GACGC 2 cut(s) 208, 626
CsiI ACCWGGT 1 cut(s) 894
CviAII CATG 3 cut(s) 469, 812, 842
DdeI CTNAG 5 cut(s) 55, 76, 91, 111, 236
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 545
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 223
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoRII CCWGG 3 cut(s) 126, 652, 894
EcoRV GATATC 1 cut(s) 253
FaeI CATG 3 cut(s) 472, 815, 845
FaiI YATR 9 cut(s) 312, 342, 470, 474, 476, 584, 760, 813, 843
FaqI GGGAC 2 cut(s) 78, 864
FatI CATG 3 cut(s) 468, 811, 841
FblI GTMKAC 3 cut(s) 96, 759, 911
Fnu4HI GCNGC 4 cut(s) 133, 153, 289, 685
Fsp4HI GCNGC 4 cut(s) 133, 153, 289, 685
FspBI CTAG 1 cut(s) 543
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 133, 153, 289, 685
GsuI CTGGAG 2 cut(s) 33, 584
HaeIII GGCC 3 cut(s) 132, 152, 225
HapII CCGG 1 cut(s) 207
HgaI GACGC 2 cut(s) 208, 626
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 472, 815, 845
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 3 cut(s) 53, 263, 578
HpaII CCGG 1 cut(s) 207
HphI GGTGA 4 cut(s) 133, 531, 626, 674
Hpy166II GTNNAC 5 cut(s) 97, 230, 259, 760, 912
Hpy188I TCNGA 6 cut(s) 58, 79, 691, 802, 828, 888
Hpy188III TCNNGA 2 cut(s) 50, 179
Hpy8I GTNNAC 5 cut(s) 97, 230, 259, 760, 912
Hpy99I CGWCG 3 cut(s) 10, 34, 224
HpyAV CCTTC 4 cut(s) 51, 166, 445, 751
HpyCH4III ACNGT 4 cut(s) 145, 375, 640, 778
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 3 cut(s) 314, 466, 841
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 290
HpyF3I CTNAG 5 cut(s) 55, 76, 91, 111, 236
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 472, 815, 845
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 1 cut(s) 844
LmnI GCTCC 3 cut(s) 111, 568, 728
Lsp1109I GCAGC 1 cut(s) 671
LweI GCATC 1 cut(s) 379
MabI ACCWGGT 1 cut(s) 894
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 139, 614
MalI GATC 1 cut(s) 846
MboI GATC 1 cut(s) 844
MboII GAAGA 5 cut(s) 36, 167, 298, 740, 759
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 5 cut(s) 182, 336, 410, 461, 630
MlyI GAGTC 2 cut(s) 47, 587
MmeI TCCRAC 4 cut(s) 437, 686, 697, 760
MseI TTAA 2 cut(s) 296, 573
MslI CAYNNNNRTG 1 cut(s) 846
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 3 cut(s) 128, 654, 896
MvaI CCWGG 3 cut(s) 128, 654, 896
MvnI CGCG 2 cut(s) 135, 291
MwoI GCNNNNNNNGC 2 cut(s) 246, 290
NdeII GATC 1 cut(s) 844
NlaIII CATG 3 cut(s) 472, 815, 845
NlaIV GGNNCC 3 cut(s) 205, 211, 417
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 139, 614
NspI RCATGY 2 cut(s) 472, 815
NspV TTCGAA 1 cut(s) 21
PasI CCCWGGG 1 cut(s) 127
PciI ACATGT 2 cut(s) 468, 811
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 4 cut(s) 134, 154, 290, 686
PleI GAGTC 2 cut(s) 47, 586
PpsI GAGTC 2 cut(s) 47, 586
PscI ACATGT 2 cut(s) 468, 811
Psp6I CCWGG 3 cut(s) 126, 652, 894
PspGI CCWGG 3 cut(s) 126, 652, 894
PspN4I GGNNCC 3 cut(s) 205, 211, 417
PspPI GGNCC 2 cut(s) 130, 415
RseI CAYNNNNRTG 1 cut(s) 846
SaqAI TTAA 2 cut(s) 296, 573
SatI GCNGC 4 cut(s) 133, 153, 289, 685
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 2 cut(s) 130, 415
SchI GAGTC 2 cut(s) 47, 587
ScrFI CCNGG 3 cut(s) 128, 654, 896
SduI GDGCHC 1 cut(s) 90
SexAI ACCWGGT 1 cut(s) 894
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 846
Sse9I AATT 5 cut(s) 182, 336, 410, 461, 630
SsiI CCGC 3 cut(s) 133, 153, 289
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 126, 652, 894
TaaI ACNGT 4 cut(s) 145, 375, 640, 778
TaiI ACGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 21, 863
TasI AATT 5 cut(s) 182, 336, 410, 461, 630
TauI GCSGC 3 cut(s) 135, 155, 291
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 2 cut(s) 296, 573
Tru9I TTAA 2 cut(s) 296, 573
TseFI GTSAC 2 cut(s) 139, 614
TseI GCWGC 1 cut(s) 684
Tsp45I GTSAC 2 cut(s) 139, 614
TspDTI ATGAA 3 cut(s) 501, 546, 905
VpaK11BI GGWCC 1 cut(s) 415
XagI CCTNNNNNAGG 1 cut(s) 114
XapI RAATTY 1 cut(s) 336
XceI RCATGY 2 cut(s) 472, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XmiI GTMKAC 3 cut(s) 96, 759, 911
XspI CTAG 1 cut(s) 543
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.