Rw1G021980

tRNA-specific adenosine deaminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005289
Physical Location & Seq
Forward (+)
46522129 .. 46524836
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021980.1

Sequence Viewer

Length: 1293 bp
ATGACGTCGCCTGGCTGCTTCGAAACGCCGTCGTTTCGTCCTTCTTCTCCAGACTCAGACTGGGGACACAAAGTCTCAGACAAGGTGCTCTCAGTCTACAACTCGCTCCCTAAGAAAGGGAAGCCCCAGGGCCGCGAAGTCACCGTCTTGGCCGCCTTCCTCCTCTCCTCTCCTCTTCAAGAATTGGAAGTTGTGGCATTGGGAACCGGAACCAAATGCGTCGGCCGTTCACTCCTCAGCCCCCACGGCGATATCGTCAACGATTCCCACGCCGAGGTCATCGCTCGCCGCGCTTTAATCAGGTTCTTCTATGCACAGATACAATGTCTTTCTCAAATTTATAGTAAGCAAAGAGATAACAATGGAAGCAAACAGTTGCGAAACGATGATGCGAAAAAGTTTATTTTTGAATTGGACCCTGATGATGGGCAAGGAAAATACACAATGAGGAAGGGTTGGAAATTGCACATGTATATATCGCAACTACCTTGTGGGATTGCTTCGCCAAGTTCATTGATGTCTCCCCCGAAAAATATCTCACCTAGCGGAGTGGGTTCATTACTGGAGGAGCTTAATGAGTCCATAAATGAGGAGGCTTTGATTGAGAATAATGGTGACGCTTCACAACTAATTGGCACGGTTCAAAGAAAGCCTGGTCGTGGTGATACTACATTATCAGTTAGCTGCTCTGACAAGATAGCCCGTTGGAATGTTGTTGGAGTTCAAGGAGCTTTGCTTTCCTTCTTTCTTCAACCTGTATACCTTTCTTCCATTACTGTTGGACAATCCCCTATCAACTCCGAAAAGGTTCATGTGGCTGATTGTCTGAAACAAGCTTTGCATGATCGTGTCCTACCATTATCAAGTGAGCTGATCAGTCCCTTTCAGGTGAACCAGCCACTACTCTGGGAAGCTCCAATGCCACCAAAGGAATTTCAACAGTCTGAGACCGCTTTGATCACTATAACATGCGGGTATTCAATATGTTGGAATAAGTCTGGTTTGCATGAAGTCATTCTTGGAACCATAGGAAGAAAGCAAGGCGCTGCAAAAGGAGCACGCAGTCCGTCTACTGAATCCCCTATATGCAAAAAACGGCTTTTGGAGATTTTCTTGTCCCTAAGGAATGAATGCCCCACCAAAATCCATGTCGATGAGATGTCGTATCGAGAAATTAAGGAAAGGGCACATGAATACAATGCAATGTCAAAAGTTCTAAAAAGAAGACATACCTTTAGTAATTGGCTATTGAAGCCCCCAGATTTGGAGGCATTCTCGGCTATGGGAGAATGA

Protein Analysis

430

Amino Acids

47.55

Weight (kDa)

8.57

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_deamin PF02137 66 - 419 9.3e-82 Adenosine-deaminase (editase) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 3 cut(s) 96, 759, 1070
AccII CGCG 2 cut(s) 135, 291
AciI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
AcoI YGGCCR 2 cut(s) 150, 223
AcsI RAATTY 2 cut(s) 336, 932
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 659, 1264
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 8 cut(s) 179, 410, 644, 725, 752, 938, 981, 1252
AhdI GACNNNNNGTC 1 cut(s) 71
AjnI CCWGG 3 cut(s) 10, 126, 652
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AluBI AGCT 6 cut(s) 571, 684, 731, 836, 871, 914
AluI AGCT 6 cut(s) 571, 684, 731, 836, 871, 914
Alw21I GWGCWC 2 cut(s) 90, 1060
Alw26I GTCTC 3 cut(s) 79, 525, 941
AoxI GGCC 3 cut(s) 130, 150, 223
ApeKI GCWGC 3 cut(s) 15, 684, 1046
ApoI RAATTY 2 cut(s) 336, 932
ArsI GACNNNNNNTTYG 4 cut(s) 129, 161, 1134, 1166
Asp700I GAANNNNTTC 2 cut(s) 807, 1014
AspLEI GCGC 2 cut(s) 293, 1046
AspS9I GGNCC 2 cut(s) 130, 415
AsuHPI GGTGA 5 cut(s) 133, 531, 626, 674, 901
AsuII TTCGAA 1 cut(s) 21
AvaII GGWCC 1 cut(s) 415
AxyI CCTNAGG 1 cut(s) 1121
BaeGI GKGCMC 1 cut(s) 1189
BbsI GAAGAC 1 cut(s) 1231
Bbv12I GWGCWC 2 cut(s) 90, 1060
BbvCI CCTCAGC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 2, 671, 1033
BccI CCATC 1 cut(s) 419
BceAI ACGGC 4 cut(s) 13, 210, 262, 1112
BciT130I CCWGG 3 cut(s) 12, 128, 654
BclI TGATCA 2 cut(s) 873, 957
BcoDI GTCTC 3 cut(s) 79, 525, 941
BfaI CTAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 1047
BglI GCCNNNNNGGC 1 cut(s) 246
BisI GCNGC 6 cut(s) 16, 133, 153, 289, 685, 1047
BlsI GCNGC 6 cut(s) 17, 134, 154, 290, 686, 1048
Bme1390I CCNGG 3 cut(s) 12, 128, 654
Bme18I GGWCC 1 cut(s) 415
BmeRI GACNNNNNGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 130, 415
BmiI GGNNCC 4 cut(s) 205, 211, 417, 1024
BmrFI CCNGG 3 cut(s) 12, 128, 654
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 1231
BplI GAGNNNNNCTC 2 cut(s) 1259, 1291
BpmI CTGGAG 2 cut(s) 33, 584
Bpu10I CCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 941
BsaJI CCNNGG 4 cut(s) 126, 127, 244, 273
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 274, 425, 659, 1264
Bse1I ACTGG 2 cut(s) 65, 567
Bse21I CCTNAGG 1 cut(s) 1121
Bse3DI GCAATG 1 cut(s) 1209
BseBI CCWGG 3 cut(s) 12, 128, 654
BseDI CCNNGG 4 cut(s) 126, 127, 244, 273
BseLI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 659, 1264
BseMI GCAATG 1 cut(s) 1209
BseMII CTCAG 5 cut(s) 69, 90, 105, 250, 936
BseNI ACTGG 2 cut(s) 65, 567
BseRI GAGGAG 6 cut(s) 152, 157, 162, 224, 581, 605
BseSI GKGCMC 1 cut(s) 1189
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 3 cut(s) 2, 671, 1033
Bsh1236I CGCG 2 cut(s) 135, 291
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 132, 152, 225
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 90, 1060
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 3 cut(s) 78, 864, 1102
BslI CCNNNNNNNGG 5 cut(s) 116, 274, 425, 659, 1264
BsmAI GTCTC 3 cut(s) 79, 525, 941
BsmFI GGGAC 3 cut(s) 78, 864, 1102
BsmI GAATGC 2 cut(s) 1136, 1271
BsnI GGCC 3 cut(s) 132, 152, 225
Bso31I GGTCTC 1 cut(s) 941
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 3 cut(s) 90, 1060, 1189
Bsp143I GATC 3 cut(s) 844, 873, 957
BspACI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
BspANI GGCC 3 cut(s) 132, 152, 225
BspCNI CTCAG 5 cut(s) 68, 89, 104, 249, 937
BspFNI CGCG 2 cut(s) 135, 291
BspLI GGNNCC 4 cut(s) 205, 211, 417, 1024
BspT104I TTCGAA 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 941
BsrDI GCAATG 1 cut(s) 1209
BsrI ACTGG 2 cut(s) 65, 567
BssECI CCNNGG 4 cut(s) 126, 127, 244, 273
BssMI GATC 3 cut(s) 844, 873, 957
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 3 cut(s) 12, 128, 654
Bst4CI ACNGT 5 cut(s) 145, 375, 640, 778, 942
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 286, 1060
BstDEI CTNAG 7 cut(s) 55, 76, 91, 111, 236, 945, 1121
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 135, 291
BstH2I RGCGCY 1 cut(s) 1047
BstHHI GCGC 2 cut(s) 293, 1046
BstKTI GATC 3 cut(s) 847, 876, 960
BstMAI GTCTC 3 cut(s) 79, 525, 941
BstMBI GATC 3 cut(s) 844, 873, 957
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
BstNI CCWGG 3 cut(s) 12, 128, 654
BstNSI RCATGY 2 cut(s) 472, 972
BstSCI CCNGG 3 cut(s) 10, 126, 652
BstSLI GKGCMC 1 cut(s) 1189
BstUI CGCG 2 cut(s) 135, 291
BstV1I GCAGC 3 cut(s) 2, 671, 1033
BstV2I GAAGAC 1 cut(s) 1231
BstXI CCANNNNNNTGG 1 cut(s) 906
BstZ17I GTATAC 1 cut(s) 760
BstZI CGGCCG 1 cut(s) 223
Bsu36I CCTNAGG 1 cut(s) 1121
BsuRI GGCC 3 cut(s) 132, 152, 225
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 286, 1060
CfoI GCGC 2 cut(s) 293, 1046
Cfr13I GGNCC 2 cut(s) 130, 415
CseI GACGC 2 cut(s) 208, 626
CviAII CATG 7 cut(s) 469, 812, 842, 969, 1007, 1148, 1190
DdeI CTNAG 7 cut(s) 55, 76, 91, 111, 236, 945, 1121
DpnI GATC 3 cut(s) 846, 875, 959
DpnII GATC 3 cut(s) 844, 873, 957
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 150, 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 180
Eam1105I GACNNNNNGTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 941
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 223
Eco81I CCTNAGG 1 cut(s) 1121
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoRII CCWGG 3 cut(s) 10, 126, 652
EcoRV GATATC 1 cut(s) 253
FaeI CATG 7 cut(s) 472, 815, 845, 972, 1010, 1151, 1193
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 3 cut(s) 78, 864, 1102
FatI CATG 7 cut(s) 468, 811, 841, 968, 1006, 1147, 1189
FauI CCCGC 1 cut(s) 965
FbaI TGATCA 2 cut(s) 873, 957
FblI GTMKAC 3 cut(s) 96, 759, 1070
Fnu4HI GCNGC 6 cut(s) 16, 133, 153, 289, 685, 1047
Fsp4HI GCNGC 6 cut(s) 16, 133, 153, 289, 685, 1047
FspBI CTAG 1 cut(s) 543
GlaI GCGC 2 cut(s) 292, 1045
GluI GCNGC 6 cut(s) 16, 133, 153, 289, 685, 1047
GsuI CTGGAG 2 cut(s) 33, 584
HaeII RGCGCY 1 cut(s) 1047
HaeIII GGCC 3 cut(s) 132, 152, 225
HapII CCGG 1 cut(s) 207
HgaI GACGC 2 cut(s) 208, 626
HhaI GCGC 2 cut(s) 293, 1046
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 472, 815, 845, 972, 1010, 1151, 1193
Hin6I GCGC 2 cut(s) 291, 1044
HinP1I GCGC 2 cut(s) 291, 1044
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 53, 263, 578, 1076
HpaII CCGG 1 cut(s) 207
HphI GGTGA 5 cut(s) 133, 531, 626, 674, 901
Hpy166II GTNNAC 6 cut(s) 97, 230, 259, 760, 892, 1071
Hpy188I TCNGA 6 cut(s) 58, 79, 691, 802, 828, 946
Hpy188III TCNNGA 3 cut(s) 50, 179, 1169
Hpy8I GTNNAC 6 cut(s) 97, 230, 259, 760, 892, 1071
Hpy99I CGWCG 3 cut(s) 10, 34, 224
HpyAV CCTTC 4 cut(s) 51, 166, 445, 751
HpyCH4III ACNGT 5 cut(s) 145, 375, 640, 778, 942
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 7 cut(s) 314, 466, 841, 1006, 1049, 1089, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
HpyF3I CTNAG 7 cut(s) 55, 76, 91, 111, 236, 945, 1121
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 472, 815, 845, 972, 1010, 1151, 1193
HspAI GCGC 2 cut(s) 291, 1044
Ksp22I TGATCA 2 cut(s) 873, 957
Kzo9I GATC 3 cut(s) 844, 873, 957
LmnI GCTCC 5 cut(s) 111, 568, 728, 919, 1055
Lsp1109I GCAGC 3 cut(s) 2, 671, 1033
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 139, 614
MalI GATC 3 cut(s) 846, 875, 959
MboI GATC 3 cut(s) 844, 873, 957
MboII GAAGA 7 cut(s) 36, 167, 298, 740, 759, 1044, 1236
MhlI GDGCHC 3 cut(s) 90, 1060, 1189
MluCI AATT 8 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240
MlyI GAGTC 2 cut(s) 47, 587
MmeI TCCRAC 5 cut(s) 437, 686, 697, 760, 968
MroXI GAANNNNTTC 2 cut(s) 807, 1014
MseI TTAA 3 cut(s) 296, 573, 1176
MslI CAYNNNNRTG 2 cut(s) 846, 1152
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 3 cut(s) 12, 128, 654
Mva1269I GAATGC 2 cut(s) 1136, 1271
MvaI CCWGG 3 cut(s) 12, 128, 654
MvnI CGCG 2 cut(s) 135, 291
MwoI GCNNNNNNNGC 5 cut(s) 246, 290, 1055, 1252, 1277
NdeII GATC 3 cut(s) 844, 873, 957
NlaIII CATG 7 cut(s) 472, 815, 845, 972, 1010, 1151, 1193
NlaIV GGNNCC 4 cut(s) 205, 211, 417, 1024
NmeAIII GCCGAG 2 cut(s) 298, 1256
NmuCI GTSAC 2 cut(s) 139, 614
NspI RCATGY 2 cut(s) 472, 972
NspV TTCGAA 1 cut(s) 21
PasI CCCWGGG 1 cut(s) 127
PciI ACATGT 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 2 cut(s) 1136, 1271
PdmI GAANNNNTTC 2 cut(s) 807, 1014
PfeI GAWTC 2 cut(s) 263, 1076
PkrI GCNGC 6 cut(s) 17, 134, 154, 290, 686, 1048
PleI GAGTC 2 cut(s) 47, 586
PpsI GAGTC 2 cut(s) 47, 586
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 10, 126, 652
PspGI CCWGG 3 cut(s) 10, 126, 652
PspN4I GGNNCC 4 cut(s) 205, 211, 417, 1024
PspPI GGNCC 2 cut(s) 130, 415
RseI CAYNNNNRTG 2 cut(s) 846, 1152
SaqAI TTAA 3 cut(s) 296, 573, 1176
SatI GCNGC 6 cut(s) 16, 133, 153, 289, 685, 1047
Sau3AI GATC 3 cut(s) 844, 873, 957
Sau96I GGNCC 2 cut(s) 130, 415
SchI GAGTC 2 cut(s) 47, 587
ScrFI CCNGG 3 cut(s) 12, 128, 654
SduI GDGCHC 3 cut(s) 90, 1060, 1189
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 846, 1152
Sse9I AATT 8 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240
SsiI CCGC 6 cut(s) 133, 153, 289, 546, 951, 972
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 10, 126, 652
TaaI ACNGT 5 cut(s) 145, 375, 640, 778, 942
TaiI ACGT 1 cut(s) 8
TaqI TCGA 3 cut(s) 21, 1152, 1168
TasI AATT 8 cut(s) 182, 336, 410, 461, 630, 932, 1173, 1240
TauI GCSGC 3 cut(s) 135, 155, 291
TfiI GAWTC 2 cut(s) 263, 1076
Tru1I TTAA 3 cut(s) 296, 573, 1176
Tru9I TTAA 3 cut(s) 296, 573, 1176
TseFI GTSAC 2 cut(s) 139, 614
TseI GCWGC 3 cut(s) 15, 684, 1046
Tsp45I GTSAC 2 cut(s) 139, 614
TspDTI ATGAA 6 cut(s) 501, 546, 800, 1023, 1143, 1206
TspGWI ACGGA 1 cut(s) 1056
VpaK11BI GGWCC 1 cut(s) 415
XagI CCTNNNNNAGG 1 cut(s) 114
XapI RAATTY 2 cut(s) 336, 932
XceI RCATGY 2 cut(s) 472, 972
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XmiI GTMKAC 3 cut(s) 96, 759, 1070
XmnI GAANNNNTTC 2 cut(s) 807, 1014
XspI CTAG 1 cut(s) 543
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.