MD07G1234400.v1.1

Transmembrane protein 87A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003014
Physical Location & Seq
Reverse (-)
30773200 .. 30777329
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1234400.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGGTCACAGAGATCTGCCGTCTCCAAATTCAAATGGATATTTTATAATCTTGATGCTGATGCACTTGATTATTTTAGCCCGAGCTTCGATCCATGACTACCAAGACGAGTCCTTCATCCGTCGTTCCAATTCCTTCTTCTTCCATGGCGGAAGTGAAGGCCTTTACGCTTCCAAGCTCGACTCTGGCAACGACAAGTCCAGCTCTGAGGATAGGCCCCTCAATGGCAAGTCATTCATCAGGTTCGAATCAATCATCTTTCGAAGAACCAAGGAATCTGCTGAAAAGAAGAATGAGATGCAGCAGAGGACTGGCTTAGTCGAGGCTATTGTTCTTGAGGTTAGGGACAGGGAGAAGATTGGGGGTTCTTATTTGAACTCCAATGCAATATGCTGCACCCCAGAGCTATCCAAGGACGGTTCTTGCAAGGTGGGAGAGGTTATCATCCAACAAAACCCAGACAACCCTGATGGGCCAAAAAGGATTCAGACCTTCTTCGAGGGGAAGAATGAAGAGGCTAATATGGATATCCAAACTATTGAAATCAACAGCACTGGAATGTACTATCTCTATTTTATGTTCTGTGATCCAGAACTTGTCGGCACATTAATCAGTGGAAGAACTGTTTGGAGGAACCCTGATGGTTATTTACCTGGAAAGATGTCCCCATTGATGACATTTTTTGGCCTAATGTCTTTAGCTTATCTAGTCCTCGGTCTTTTCTGGTTTCTCCGCTTTTTACAGTATTGGAAAGACATAATACAGTTGCACTACCACATCAGTGCTGTAATTGGACTTGGAATGTGTGAAATGGCCCTTTGGTATTTTGAATATGCAAATTTCAATTCCACTGGAAGCAGACCAATGGGAATTACCATATGGGCAGTAACCTTCAGCGCCGTTAAAAAAACTGTCTCCCGGCTTCTCCTTCTGGTGGTTTCAATGGGCTATGGTGTTGTGCGGCCAACTCTCGGTGGTATAACCTCGAAAGTACTTCTCCTTGGTGTGATATATTTTGTAGCATCAGAAGCACTTGAGCTTGTTGAGCATTTGGGGAATATCAACGACTTTTCTGGAAAAGCAAGGCTTTTTCTGGTGCTGCCTGTTGCTCTTTTGGATGCCTGTTTTATTCTTTGGATCTTTTCCTCGTTATCTAAAACTTTGGAGAAGCTTCAGATTCGAAGAAGCATGGCCAAACTTGAGCTGTACCGAAAGTTTACGAATTCTCTTGCAGTATCAGTGCTGCTCTCTGTTGCTTGGATTGGCTATGAGTTGTATTTCAATGCCACTGACCCGTTGAGTGAGCTGTGGCGAAGAGCCTGGGTCATCCCAGCTTTCTGGACTTTGCTTGCTTATTTGCTGTTGGTGGTGATATGTATTCTTTGGGCTCCATCTCACAACCCAACCGGATATGCATACTCGGAGGAGACCGCGGATGACTTTGATGACGAGGGTATCTCGCTCACGGGAAGTGGAGTTAAGGTGGCGGGCGGAGACTTGGCAACCAAGCTAGAAAGAAAGGAAAGGAAGGCGTCGAGTGCAGCAGAACATCACGTGTTTGGGCTTGGGGAAGATGTAGAGGAAGACAAGAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lung_7-TM_R

Protein Analysis

533

Amino Acids

59.97

Weight (kDa)

5.9

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 223 - 468 1.9e-95 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AccII CGCG 1 cut(s) 1433
AciI CCGC 7 cut(s) 150, 733, 961, 1431, 1433, 1487, 1491
AclWI GGATC 3 cut(s) 85, 581, 1145
AcoI YGGCCR 2 cut(s) 962, 1191
AcsI RAATTY 3 cut(s) 28, 838, 1222
AcuI CTGAAG 2 cut(s) 877, 1157
AcvI CACGTG 1 cut(s) 1555
AcyI GRCGYC 1 cut(s) 1532
AfaI GTAC 3 cut(s) 563, 993, 1208
AfiI CCNNNNNNNGG 3 cut(s) 224, 934, 971
AflIII ACRYGT 1 cut(s) 1554
AgsI TTSAA 7 cut(s) 33, 376, 542, 830, 844, 942, 1282
AjnI CCWGG 2 cut(s) 652, 1319
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 610, 642
AleI CACNNNNGTG 1 cut(s) 780
Alw26I GTCTC 4 cut(s) 27, 919, 1421, 1488
AlwI GGATC 3 cut(s) 85, 581, 1145
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 7 cut(s) 160, 215, 473, 685, 813, 962, 1191
ApeKI GCWGC 5 cut(s) 301, 393, 1099, 1243, 1541
ApoI RAATTY 3 cut(s) 28, 838, 1222
AseI ATTAAT 1 cut(s) 608
AspLEI GCGC 1 cut(s) 899
AspS9I GGNCC 3 cut(s) 216, 473, 814
AsuC2I CCSGG 1 cut(s) 919
AsuHPI GGTGA 1 cut(s) 1381
AsuII TTCGAA 3 cut(s) 246, 262, 1180
AvaI CYCGRG 1 cut(s) 81
BaeI ACNNNNGTAYC 2 cut(s) 1438, 1471
BalI TGGCCA 1 cut(s) 1193
BanII GRGCYC 1 cut(s) 1390
BbrPI CACGTG 1 cut(s) 1555
BbsI GAAGAC 1 cut(s) 1590
BbvI GCAGC 5 cut(s) 313, 380, 1086, 1230, 1553
BccI CCATC 3 cut(s) 464, 635, 1399
BceAI ACGGC 2 cut(s) 4, 884
BciT130I CCWGG 2 cut(s) 654, 1321
BcnI CCSGG 1 cut(s) 919
BcoDI GTCTC 4 cut(s) 27, 919, 1421, 1488
BfaI CTAG 2 cut(s) 707, 1511
BfoI RGCGCY 1 cut(s) 900
BglII AGATCT 1 cut(s) 13
BisI GCNGC 6 cut(s) 302, 394, 962, 1100, 1244, 1542
BlsI GCNGC 6 cut(s) 303, 395, 963, 1101, 1245, 1543
BmcAI AGTACT 1 cut(s) 993
Bme1390I CCNGG 3 cut(s) 654, 919, 1321
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 216, 473, 814
BmiI GGNNCC 3 cut(s) 218, 635, 1389
BmrFI CCNGG 3 cut(s) 654, 919, 1321
BmsI GCATC 5 cut(s) 45, 51, 288, 1031, 1108
BpiI GAAGAC 1 cut(s) 1590
BplI GAGNNNNNCTC 2 cut(s) 1442, 1474
Bpu14I TTCGAA 3 cut(s) 246, 262, 1180
BpuEI CTTGAG 3 cut(s) 356, 1055, 1220
BpuMI CCSGG 1 cut(s) 919
BsaAI YACGTR 1 cut(s) 1555
BsaHI GRCGYC 1 cut(s) 1532
BsaI GGTCTC 1 cut(s) 1421
BsaJI CCNNGG 7 cut(s) 145, 270, 411, 712, 1000, 1320, 1431
BsaWI WCCGGW 1 cut(s) 1406
BsaXI ACNNNNNCTCC 2 cut(s) 1467, 1497
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 934, 971
Bse1I ACTGG 3 cut(s) 316, 559, 856
BseBI CCWGG 2 cut(s) 654, 1321
BseDI CCNNGG 7 cut(s) 145, 270, 411, 712, 1000, 1320, 1431
BseGI GGATG 5 cut(s) 117, 444, 1123, 1326, 1441
BseLI CCNNNNNNNGG 3 cut(s) 224, 934, 971
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 3 cut(s) 316, 559, 856
BseRI GAGGAG 1 cut(s) 1439
BseXI GCAGC 5 cut(s) 313, 380, 1086, 1230, 1553
BseYI CCCAGC 1 cut(s) 1330
BsgI GTGCAG 2 cut(s) 379, 1560
Bsh1236I CGCG 1 cut(s) 1433
BshFI GGCC 7 cut(s) 162, 217, 475, 687, 815, 964, 1193
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 2 cut(s) 919, 1407
BslFI GGGAC 2 cut(s) 359, 649
BslI CCNNNNNNNGG 3 cut(s) 224, 934, 971
BsmAI GTCTC 4 cut(s) 27, 919, 1421, 1488
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 359, 649
BsnI GGCC 7 cut(s) 162, 217, 475, 687, 815, 964, 1193
Bso31I GGTCTC 1 cut(s) 1421
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 3 cut(s) 246, 262, 1180
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp143I GATC 4 cut(s) 13, 90, 586, 1137
Bsp19I CCATGG 1 cut(s) 145
BspACI CCGC 7 cut(s) 150, 733, 961, 1431, 1433, 1487, 1491
BspANI GGCC 7 cut(s) 162, 217, 475, 687, 815, 964, 1193
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 1433
BspLI GGNNCC 3 cut(s) 218, 635, 1389
BspPI GGATC 3 cut(s) 85, 581, 1145
BspQI GCTCTTC 1 cut(s) 1309
BspT104I TTCGAA 3 cut(s) 246, 262, 1180
BspTNI GGTCTC 1 cut(s) 1421
BsrI ACTGG 3 cut(s) 316, 559, 856
BssECI CCNNGG 7 cut(s) 145, 270, 411, 712, 1000, 1320, 1431
BssMI GATC 4 cut(s) 13, 90, 586, 1137
BssNI GRCGYC 1 cut(s) 1532
BssT1I CCWWGG 4 cut(s) 145, 270, 411, 1000
Bst2UI CCWGG 2 cut(s) 654, 1321
Bst4CI ACNGT 5 cut(s) 419, 625, 744, 765, 913
Bst6I CTCTTC 2 cut(s) 507, 1309
BstACI GRCGYC 1 cut(s) 1532
BstBAI YACGTR 1 cut(s) 1555
BstBI TTCGAA 3 cut(s) 246, 262, 1180
BstC8I GCNNGC 2 cut(s) 1350, 1489
BstDEI CTNAG 2 cut(s) 207, 316
BstDSI CCRYGG 2 cut(s) 145, 1431
BstF5I GGATG 5 cut(s) 117, 444, 1123, 1326, 1441
BstFNI CGCG 1 cut(s) 1433
BstH2I RGCGCY 1 cut(s) 900
BstHHI GCGC 1 cut(s) 899
BstKTI GATC 4 cut(s) 16, 93, 589, 1140
BstMAI GTCTC 4 cut(s) 27, 919, 1421, 1488
BstMBI GATC 4 cut(s) 13, 90, 586, 1137
BstMWI GCNNNNNNNGC 3 cut(s) 1028, 1045, 1538
BstNI CCWGG 2 cut(s) 654, 1321
BstSCI CCNGG 3 cut(s) 652, 917, 1319
BstUI CGCG 1 cut(s) 1433
BstV1I GCAGC 5 cut(s) 313, 380, 1086, 1230, 1553
BstV2I GAAGAC 1 cut(s) 1590
BstX2I RGATCY 2 cut(s) 13, 1137
BstXI CCANNNNNNTGG 1 cut(s) 1338
BstYI RGATCY 2 cut(s) 13, 1137
BsuRI GGCC 7 cut(s) 162, 217, 475, 687, 815, 964, 1193
BtgI CCRYGG 2 cut(s) 145, 1431
BtsCI GGATG 5 cut(s) 117, 444, 1123, 1326, 1441
BtsIMutI CAGTG 6 cut(s) 552, 619, 787, 849, 1245, 1287
Cac8I GCNNGC 2 cut(s) 1350, 1489
CfoI GCGC 1 cut(s) 899
Cfr13I GGNCC 3 cut(s) 216, 473, 814
Cfr42I CCGCGG 1 cut(s) 1434
CseI GACGC 1 cut(s) 1521
Csp6I GTAC 3 cut(s) 562, 992, 1207
CviAII CATG 3 cut(s) 95, 146, 1189
CviQI GTAC 3 cut(s) 562, 992, 1207
DdeI CTNAG 2 cut(s) 207, 316
DpnI GATC 4 cut(s) 15, 92, 588, 1139
DpnII GATC 4 cut(s) 13, 90, 586, 1137
EaeI YGGCCR 2 cut(s) 962, 1191
Eam1104I CTCTTC 2 cut(s) 507, 1309
EarI CTCTTC 2 cut(s) 507, 1309
EciI GGCGGA 2 cut(s) 165, 1506
Eco130I CCWWGG 4 cut(s) 145, 270, 411, 1000
Eco147I AGGCCT 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 1390
Eco31I GGTCTC 1 cut(s) 1421
Eco32I GATATC 1 cut(s) 529
Eco57I CTGAAG 2 cut(s) 877, 1157
Eco72I CACGTG 1 cut(s) 1555
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 216
EcoRI GAATTC 1 cut(s) 1222
EcoRII CCWGG 2 cut(s) 652, 1319
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 4 cut(s) 145, 270, 411, 1000
EcoT22I ATGCAT 1 cut(s) 1417
EcoT38I GRGCYC 1 cut(s) 1390
ErhI CCWWGG 4 cut(s) 145, 270, 411, 1000
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 3 cut(s) 98, 149, 1192
FalI AAGNNNNNCTT 2 cut(s) 1071, 1103
FaqI GGGAC 2 cut(s) 359, 649
FatI CATG 3 cut(s) 94, 145, 1188
FauI CCCGC 1 cut(s) 1480
FauNDI CATATG 1 cut(s) 878
Fnu4HI GCNGC 6 cut(s) 302, 394, 962, 1100, 1244, 1542
FokI GGATG 5 cut(s) 104, 431, 1130, 1313, 1448
FriOI GRGCYC 1 cut(s) 1390
Fsp4HI GCNGC 6 cut(s) 302, 394, 962, 1100, 1244, 1542
FspBI CTAG 2 cut(s) 707, 1511
GlaI GCGC 1 cut(s) 898
GluI GCNGC 6 cut(s) 302, 394, 962, 1100, 1244, 1542
GsaI CCCAGC 1 cut(s) 1334
HaeII RGCGCY 1 cut(s) 900
HaeIII GGCC 7 cut(s) 162, 217, 475, 687, 815, 964, 1193
HapII CCGG 2 cut(s) 919, 1407
HgaI GACGC 1 cut(s) 1521
HhaI GCGC 1 cut(s) 899
Hin1I GRCGYC 1 cut(s) 1532
Hin1II CATG 3 cut(s) 98, 149, 1192
Hin6I GCGC 1 cut(s) 897
HinP1I GCGC 1 cut(s) 897
HindIII AAGCTT 1 cut(s) 1169
HinfI GANTC 6 cut(s) 110, 182, 248, 275, 484, 1177
HpaII CCGG 2 cut(s) 919, 1407
HphI GGTGA 1 cut(s) 1381
Hpy166II GTNNAC 1 cut(s) 1218
Hpy188I TCNGA 5 cut(s) 208, 489, 1027, 1176, 1423
Hpy188III TCNNGA 5 cut(s) 52, 335, 590, 1074, 1339
Hpy8I GTNNAC 1 cut(s) 1218
Hpy99I CGWCG 2 cut(s) 126, 1537
HpyAV CCTTC 7 cut(s) 124, 145, 152, 502, 901, 938, 1522
HpyCH4III ACNGT 5 cut(s) 419, 625, 744, 765, 913
HpyCH4IV ACGT 1 cut(s) 1554
HpyF10VI GCNNNNNNNGC 3 cut(s) 1028, 1045, 1538
HpyF3I CTNAG 2 cut(s) 207, 316
HpySE526I ACGT 1 cut(s) 1554
Hsp92I GRCGYC 1 cut(s) 1532
Hsp92II CATG 3 cut(s) 98, 149, 1192
HspAI GCGC 1 cut(s) 897
KspI CCGCGG 1 cut(s) 1434
Kzo9I GATC 4 cut(s) 13, 90, 586, 1137
LguI GCTCTTC 1 cut(s) 1309
LmnI GCTCC 1 cut(s) 1393
Lsp1109I GCAGC 5 cut(s) 313, 380, 1086, 1230, 1553
LweI GCATC 5 cut(s) 45, 51, 288, 1031, 1108
MaeI CTAG 2 cut(s) 707, 1511
MaeII ACGT 1 cut(s) 1554
MaeIII GTNAC 2 cut(s) 5, 886
MalI GATC 4 cut(s) 15, 92, 588, 1139
MboI GATC 4 cut(s) 13, 90, 586, 1137
MflI RGATCY 2 cut(s) 13, 1137
MhlI GDGCHC 1 cut(s) 1390
MlsI TGGCCA 1 cut(s) 1193
MluCI AATT 7 cut(s) 28, 130, 789, 838, 844, 870, 1222
MluNI TGGCCA 1 cut(s) 1193
MlyI GAGTC 2 cut(s) 119, 176
MmeI TCCRAC 1 cut(s) 472
Mox20I TGGCCA 1 cut(s) 1193
Mph1103I ATGCAT 1 cut(s) 1417
MscI TGGCCA 1 cut(s) 1193
MseI TTAA 3 cut(s) 608, 903, 1479
MslI CAYNNNNRTG 2 cut(s) 557, 780
Msp20I TGGCCA 1 cut(s) 1193
MspA1I CMGCKG 1 cut(s) 1433
MspI CCGG 2 cut(s) 919, 1407
MspR9I CCNGG 3 cut(s) 654, 919, 1321
MvaI CCWGG 2 cut(s) 654, 1321
MvnI CGCG 1 cut(s) 1433
MwoI GCNNNNNNNGC 3 cut(s) 1028, 1045, 1538
NciI CCSGG 1 cut(s) 919
NcoI CCATGG 1 cut(s) 145
NdeI CATATG 1 cut(s) 878
NdeII GATC 4 cut(s) 13, 90, 586, 1137
NlaIII CATG 3 cut(s) 98, 149, 1192
NlaIV GGNNCC 3 cut(s) 218, 635, 1389
NmuCI GTSAC 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 1417
NspV TTCGAA 3 cut(s) 246, 262, 1180
OliI CACNNNNGTG 1 cut(s) 780
PceI AGGCCT 1 cut(s) 162
PciSI GCTCTTC 1 cut(s) 1309
PfeI GAWTC 4 cut(s) 248, 275, 484, 1177
PkrI GCNGC 6 cut(s) 303, 395, 963, 1101, 1245, 1543
PleI GAGTC 2 cut(s) 118, 176
PmaCI CACGTG 1 cut(s) 1555
PmlI CACGTG 1 cut(s) 1555
PpsI GAGTC 2 cut(s) 118, 176
Ppu21I YACGTR 1 cut(s) 1555
PshBI ATTAAT 1 cut(s) 608
PsiI TTATAA 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 652, 1319
PspCI CACGTG 1 cut(s) 1555
PspFI CCCAGC 1 cut(s) 1330
PspGI CCWGG 2 cut(s) 652, 1319
PspN4I GGNNCC 3 cut(s) 218, 635, 1389
PspPI GGNCC 3 cut(s) 216, 473, 814
PsuI RGATCY 2 cut(s) 13, 1137
RsaI GTAC 3 cut(s) 563, 993, 1208
RsaNI GTAC 3 cut(s) 562, 992, 1207
RseI CAYNNNNRTG 2 cut(s) 557, 780
SacII CCGCGG 1 cut(s) 1434
SapI GCTCTTC 1 cut(s) 1309
SaqAI TTAA 3 cut(s) 608, 903, 1479
SatI GCNGC 6 cut(s) 302, 394, 962, 1100, 1244, 1542
Sau3AI GATC 4 cut(s) 13, 90, 586, 1137
Sau96I GGNCC 3 cut(s) 216, 473, 814
ScaI AGTACT 1 cut(s) 993
SchI GAGTC 2 cut(s) 119, 176
ScrFI CCNGG 3 cut(s) 654, 919, 1321
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 5 cut(s) 45, 51, 288, 1031, 1108
Sfr303I CCGCGG 1 cut(s) 1434
SfuI TTCGAA 3 cut(s) 246, 262, 1180
SgrBI CCGCGG 1 cut(s) 1434
SmiMI CAYNNNNRTG 2 cut(s) 557, 780
SmlI CTYRAG 3 cut(s) 335, 1034, 1199
SmoI CTYRAG 3 cut(s) 335, 1034, 1199
Sse9I AATT 7 cut(s) 28, 130, 789, 838, 844, 870, 1222
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 7 cut(s) 150, 733, 961, 1431, 1433, 1487, 1491
SspMI CTAG 2 cut(s) 707, 1511
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 652, 917, 1319
StyI CCWWGG 4 cut(s) 145, 270, 411, 1000
TaaI ACNGT 5 cut(s) 419, 625, 744, 765, 913
TaiI ACGT 1 cut(s) 1557
TaqI TCGA 9 cut(s) 89, 180, 246, 262, 321, 498, 986, 1180, 1535
TaqII GACCGA 1 cut(s) 704
TasI AATT 7 cut(s) 28, 130, 789, 838, 844, 870, 1222
TatI WGTACW 2 cut(s) 561, 991
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 4 cut(s) 248, 275, 484, 1177
Tru1I TTAA 3 cut(s) 608, 903, 1479
Tru9I TTAA 3 cut(s) 608, 903, 1479
TscAI CASTG 6 cut(s) 559, 619, 787, 856, 1245, 1294
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 5 cut(s) 301, 393, 1099, 1243, 1541
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 3 cut(s) 106, 226, 525
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 6 cut(s) 559, 619, 787, 856, 1245, 1294
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 3 cut(s) 28, 838, 1222
XspI CTAG 2 cut(s) 707, 1511
ZrmI AGTACT 1 cut(s) 993
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.