RLG00000025015

Transmembrane protein 87B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003014
Physical Location & Seq
Reverse (-)
41326749 .. 41329708
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025015

Sequence Viewer

Length: 1557 bp
ATGGAACGAGCCAGATGGTTTTGGGGAGAAAAGGGGCCAGCTTTTGGGTTCGTCATAGTTGGGCTCATTTTGATCATCAACGGCTGTAATGCTTCCATCCACGAGTACAGAAACGAAGCTTTTACTCCTCAGTCAAACGCCTTCTTCTTCCATGGAGGCAGCGAAGGTCTCTACGCTTCTAAGGTCGTCGATTCCTCTTCTTCTTCAGAGACTCACGTCAAGGGCAAGTCCTTCATCAGGTTTGAGAGTGTTACTTTTGTGAGGACAAAGGAGTCTGCGAATAAGCAGAATGAAATGCAGCAGAGTGCTGGTTTGGTGGAAGCTATTATTCTTGAGGTGAAGGACAGGGCTAGGATTGGAGCTTCGTTCCTGAAATCGGATCTCATATGCTGCACCAGGAATCTTTCCGAGGATGGACAATGCAAGGCGGGGGAGGTTGTTATCCACAAAAATCCAGACAACCCCGAGTGGCCTAGGCGCATCCAAACATTCTTTAATGGAAAGAACGAAGAGGCTAAAATGAACTCTGAGACGGTTGAGATAAAAAGTTCTGGAATGTACTACCTGTATTTTATGTTTTGTAATCCGGATCTCAAGGGCACGCTGATTAAAGGCAGGACCGATTGGAGAAATCCATATGGTTATTTGCCGGGGAAGATGGCTCCTTTGATGACATTCTATGGCTTAATGTCTTTAGCTTACCTTGTGCTTGGCCTTGCCTGGTTTCTGAGGTTTGTTCAGTTTTGGAAGGATATCATACAATTGCACTACCATATCACAGCGGTGATTGCTCTTGGAATGTGTGAAATGGCCGTCTGGTACTTTGAGTACGTCAATTTCAATTCGACTGGAACCAGACCTATGGGCATTACATTGTGGGCGGTAACCTTTAGCTCTGTCAAGAAGACACTCTCTCGTCTTCTTCTGTTAGTAGTTTCAATGGGCTTTGGTGTGGTGAAGCCTACACTTGGTGGTATAACCCCAAAAGTATTTCTGCTTGGCGTGGTATATTTTATGGCATCAGAAGCACTCGAGCTTATTGAAAATTTGGGGAATATCAATGATTTTTCTGGAAAAGCAAAGTTATTTTTGGTCTTACCAGTTGCCTTCTTAGATTCGTGCTTTATTCTGTGGATTTTCTCTTCGTTATCAAAAACTTTGGAGAAACTTCAGATCAGGAGAAACATGGCTAAGTTGGAGCTATATCGAAAATTTACCAACTCTCTTGCAGTGTCTGTGCTTCTGTCAGTGGCCTGGATTGGCTTTGAGCTATATTTCAATGCAACCGATCCATTGAGTGAGTTTTGGCAAATCGCCTGGGTTATTCCTGCATTTTGGACTCTGCTTGCCTACGCTCTCTTGGCAGTGATATGTGTACTCTGGGCTCCATCAAGTAACCCAACTAGATATGCATACTTAGAGGAGACGGGAGATGACTTTGATGAGGAGGGCGTCTCGTTAACAACTGGTGCATTGAAGGTGAGCGGAGATACAGCAGCTATGCGAGAATTTAACAATGGGCTTGCAGAAGATCTGGAAGAGGATAAGCGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0008150 GO:0016192 GO:0016197 GO:0016482 GO:0042147 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lung_7-TM_R

Protein Analysis

519

Amino Acids

58.43

Weight (kDa)

6.18

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 222 - 467 1.1e-93 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 1485
AccIII TCCGGA 1 cut(s) 586
AciI CCGC 4 cut(s) 428, 782, 881, 1485
AclWI GGATC 3 cut(s) 387, 597, 1283
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 3 cut(s) 1045, 1211, 1508
AcuI CTGAAG 2 cut(s) 189, 1154
AcyI GRCGYC 1 cut(s) 1452
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 5 cut(s) 107, 560, 821, 830, 1377
AfiI CCNNNNNNNGG 4 cut(s) 44, 237, 376, 968
AgsI TTSAA 5 cut(s) 841, 939, 1043, 1279, 1477
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 4 cut(s) 395, 719, 1253, 1316
AleI CACNNNNGTG 1 cut(s) 782
Alw26I GTCTC 5 cut(s) 173, 203, 524, 1418, 1459
AlwI GGATC 3 cut(s) 387, 597, 1283
AlwNI CAGNNNCTG 1 cut(s) 1235
Ama87I CYCGRG 2 cut(s) 464, 1031
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 5 cut(s) 35, 470, 712, 810, 1251
ApeKI GCWGC 4 cut(s) 159, 298, 390, 1496
ApoI RAATTY 3 cut(s) 1045, 1211, 1508
AspA2I CCTAGG 1 cut(s) 473
AspLEI GCGC 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 35, 618
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 4 cut(s) 349, 796, 967, 1492
AvaI CYCGRG 2 cut(s) 464, 1031
AvaII GGWCC 1 cut(s) 618
AvrII CCTAGG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 602
BanII GRGCYC 2 cut(s) 66, 1387
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 2 cut(s) 911, 911
BbvI GCAGC 4 cut(s) 171, 310, 377, 1508
BccI CCATC 5 cut(s) 9, 104, 407, 652, 1396
BceAI ACGGC 2 cut(s) 97, 797
BciT130I CCWGG 4 cut(s) 397, 721, 1255, 1318
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 651
BcoDI GTCTC 5 cut(s) 173, 203, 524, 1418, 1459
BfaI CTAG 3 cut(s) 351, 474, 1404
BglII AGATCT 1 cut(s) 1531
BisI GCNGC 4 cut(s) 160, 299, 391, 1497
BlnI CCTAGG 1 cut(s) 473
BlsI GCNGC 4 cut(s) 161, 300, 392, 1498
Bme1390I CCNGG 5 cut(s) 397, 651, 721, 1255, 1318
Bme18I GGWCC 1 cut(s) 618
BmeT110I CYCGRG 2 cut(s) 464, 1031
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 2 cut(s) 35, 618
BmiI GGNNCC 4 cut(s) 36, 663, 853, 1386
BmrFI CCNGG 5 cut(s) 397, 651, 721, 1255, 1318
BmsI GCATC 2 cut(s) 489, 1028
BoxI GACNNNNGTC 1 cut(s) 215
BpiI GAAGAC 2 cut(s) 911, 911
BplI GAGNNNNNCTC 2 cut(s) 1439, 1471
BpuEI CTTGAG 2 cut(s) 353, 578
BpuMI CCSGG 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 1452
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 5 cut(s) 151, 408, 473, 650, 1317
BsaWI WCCGGW 1 cut(s) 586
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 237, 376, 968
Bse1I ACTGG 3 cut(s) 853, 1100, 1471
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 4 cut(s) 397, 721, 1255, 1318
BseDI CCNNGG 5 cut(s) 151, 408, 473, 650, 1317
BseGI GGATG 3 cut(s) 96, 418, 480
BseLI CCNNNNNNNGG 4 cut(s) 44, 237, 376, 968
BseMII CTCAG 3 cut(s) 143, 519, 719
BseNI ACTGG 3 cut(s) 853, 1100, 1471
BseRI GAGGAG 3 cut(s) 117, 1436, 1460
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 4 cut(s) 171, 310, 377, 1508
BsgI GTGCAG 1 cut(s) 376
BshFI GGCC 5 cut(s) 37, 472, 714, 812, 1253
BsiHKCI CYCGRG 2 cut(s) 464, 1031
BsiSI CCGG 2 cut(s) 587, 650
BslI CCNNNNNNNGG 4 cut(s) 44, 237, 376, 968
BsmAI GTCTC 5 cut(s) 173, 203, 524, 1418, 1459
BsmBI CGTCTC 3 cut(s) 524, 1418, 1459
BsnI GGCC 5 cut(s) 37, 472, 714, 812, 1253
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 464, 1031
Bsp1286I GDGCHC 3 cut(s) 66, 602, 1387
Bsp13I TCCGGA 1 cut(s) 586
Bsp143I GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 4 cut(s) 428, 782, 881, 1485
BspANI GGCC 5 cut(s) 37, 472, 714, 812, 1253
BspCNI CTCAG 3 cut(s) 142, 520, 720
BspEI TCCGGA 1 cut(s) 586
BspLI GGNNCC 4 cut(s) 36, 663, 853, 1386
BspPI GGATC 3 cut(s) 387, 597, 1283
BspTNI GGTCTC 1 cut(s) 173
BsrBI CCGCTC 1 cut(s) 1485
BsrI ACTGG 3 cut(s) 853, 1100, 1471
BssECI CCNNGG 5 cut(s) 151, 408, 473, 650, 1317
BssMI GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
BssNI GRCGYC 1 cut(s) 1452
BssSI CACGAG 1 cut(s) 101
BssT1I CCWWGG 2 cut(s) 151, 473
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 4 cut(s) 397, 721, 1255, 1318
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 4 cut(s) 202, 504, 1147, 1533
BstACI GRCGYC 1 cut(s) 1452
BstC8I GCNNGC 4 cut(s) 39, 602, 1347, 1524
BstDEI CTNAG 7 cut(s) 129, 180, 528, 728, 1111, 1191, 1417
BstDSI CCRYGG 1 cut(s) 151
BstEII GGTNACC 1 cut(s) 883
BstENI CCTNNNNNAGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 96, 418, 480
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 6 cut(s) 75, 382, 592, 1176, 1291, 1534
BstMAI GTCTC 5 cut(s) 173, 203, 524, 1418, 1459
BstMBI GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
BstMWI GCNNNNNNNGC 3 cut(s) 788, 1025, 1361
BstNI CCWGG 4 cut(s) 397, 721, 1255, 1318
BstPAI GACNNNNGTC 1 cut(s) 215
BstPI GGTNACC 1 cut(s) 883
BstSCI CCNGG 5 cut(s) 395, 649, 719, 1253, 1316
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 4 cut(s) 171, 310, 377, 1508
BstV2I GAAGAC 2 cut(s) 911, 911
BstX2I RGATCY 3 cut(s) 379, 589, 1531
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 3 cut(s) 379, 589, 1531
BsuRI GGCC 5 cut(s) 37, 472, 714, 812, 1253
BtgI CCRYGG 1 cut(s) 151
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 96, 418, 480
BtsI GCAGTG 2 cut(s) 1236, 1371
BtsIMutI CAGTG 3 cut(s) 1236, 1254, 1371
Cac8I GCNNGC 4 cut(s) 39, 602, 1347, 1524
CaiI CAGNNNCTG 1 cut(s) 1235
CfoI GCGC 1 cut(s) 480
Cfr13I GGNCC 2 cut(s) 35, 618
CseI GACGC 1 cut(s) 1441
Csp6I GTAC 5 cut(s) 106, 559, 820, 829, 1376
CviAII CATG 2 cut(s) 152, 1186
CviQI GTAC 5 cut(s) 106, 559, 820, 829, 1376
DdeI CTNAG 7 cut(s) 129, 180, 528, 728, 1111, 1191, 1417
DpnI GATC 6 cut(s) 74, 381, 591, 1175, 1290, 1533
DpnII GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
DraIII CACNNNGTG 1 cut(s) 971
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 4 cut(s) 202, 504, 1147, 1533
EarI CTCTTC 4 cut(s) 202, 504, 1147, 1533
Eco130I CCWWGG 2 cut(s) 151, 473
Eco24I GRGCYC 2 cut(s) 66, 1387
Eco31I GGTCTC 1 cut(s) 173
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 1 cut(s) 618
Eco57I CTGAAG 2 cut(s) 189, 1154
Eco88I CYCGRG 2 cut(s) 464, 1031
Eco91I GGTNACC 1 cut(s) 883
EcoNI CCTNNNNNAGG 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 883
EcoRII CCWGG 4 cut(s) 395, 719, 1253, 1316
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 2 cut(s) 151, 473
EcoT22I ATGCAT 1 cut(s) 1414
EcoT38I GRGCYC 2 cut(s) 66, 1387
ErhI CCWWGG 2 cut(s) 151, 473
Esp3I CGTCTC 3 cut(s) 524, 1418, 1459
FaeI CATG 2 cut(s) 155, 1189
FatI CATG 2 cut(s) 151, 1185
FauI CCCGC 1 cut(s) 421
FauNDI CATATG 2 cut(s) 386, 637
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 160, 299, 391, 1497
FokI GGATG 3 cut(s) 83, 425, 467
FriOI GRGCYC 2 cut(s) 66, 1387
Fsp4HI GCNGC 4 cut(s) 160, 299, 391, 1497
FspBI CTAG 3 cut(s) 351, 474, 1404
GlaI GCGC 1 cut(s) 479
GluI GCNGC 4 cut(s) 160, 299, 391, 1497
HaeIII GGCC 5 cut(s) 37, 472, 714, 812, 1253
HapII CCGG 2 cut(s) 587, 650
HgaI GACGC 1 cut(s) 1441
HhaI GCGC 1 cut(s) 480
Hin1I GRCGYC 1 cut(s) 1452
Hin1II CATG 2 cut(s) 155, 1189
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HincII GTYRAC 1 cut(s) 1461
HindII GTYRAC 1 cut(s) 1461
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 6 cut(s) 191, 211, 272, 400, 1115, 1339
HpaI GTTAAC 1 cut(s) 1461
HpaII CCGG 2 cut(s) 587, 650
HphI GGTGA 4 cut(s) 349, 796, 967, 1492
Hpy166II GTNNAC 2 cut(s) 1376, 1461
Hpy188I TCNGA 7 cut(s) 208, 379, 409, 529, 729, 1024, 1173
Hpy188III TCNNGA 9 cut(s) 332, 370, 455, 552, 587, 901, 1071, 1177, 1535
Hpy8I GTNNAC 2 cut(s) 1376, 1461
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 7 cut(s) 151, 158, 241, 334, 742, 1117, 1471
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 2 cut(s) 216, 831
HpyF10VI GCNNNNNNNGC 3 cut(s) 788, 1025, 1361
HpyF3I CTNAG 7 cut(s) 129, 180, 528, 728, 1111, 1191, 1417
HpySE526I ACGT 2 cut(s) 216, 831
Hsp92I GRCGYC 1 cut(s) 1452
Hsp92II CATG 2 cut(s) 155, 1189
HspAI GCGC 1 cut(s) 478
Kpn2I TCCGGA 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 72
KspAI GTTAAC 1 cut(s) 1461
Kzo9I GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
LmnI GCTCC 4 cut(s) 359, 667, 1198, 1390
Lsp1109I GCAGC 4 cut(s) 171, 310, 377, 1508
LweI GCATC 2 cut(s) 489, 1028
MaeI CTAG 3 cut(s) 351, 474, 1404
MaeII ACGT 2 cut(s) 216, 831
MaeIII GTNAC 3 cut(s) 250, 883, 1394
MalI GATC 6 cut(s) 74, 381, 591, 1175, 1290, 1533
MbiI CCGCTC 1 cut(s) 1485
MboI GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 3 cut(s) 379, 589, 1531
MhlI GDGCHC 3 cut(s) 66, 602, 1387
MluCI AATT 6 cut(s) 761, 835, 841, 1045, 1211, 1508
MlyI GAGTC 3 cut(s) 205, 281, 1333
MmeI TCCRAC 1 cut(s) 1176
Mph1103I ATGCAT 1 cut(s) 1414
MroI TCCGGA 1 cut(s) 586
MseI TTAA 5 cut(s) 495, 609, 686, 1460, 1512
MslI CAYNNNNRTG 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 2 cut(s) 587, 650
MspR9I CCNGG 5 cut(s) 397, 651, 721, 1255, 1318
MunI CAATTG 1 cut(s) 761
MvaI CCWGG 4 cut(s) 397, 721, 1255, 1318
MwoI GCNNNNNNNGC 3 cut(s) 788, 1025, 1361
NciI CCSGG 1 cut(s) 651
NcoI CCATGG 1 cut(s) 151
NdeI CATATG 2 cut(s) 386, 637
NdeII GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
NlaIII CATG 2 cut(s) 155, 1189
NlaIV GGNNCC 4 cut(s) 36, 663, 853, 1386
NsiI ATGCAT 1 cut(s) 1414
OliI CACNNNNGTG 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 1031
PfeI GAWTC 3 cut(s) 191, 400, 1115
PflMI CCANNNNNTGG 1 cut(s) 44
PkrI GCNGC 4 cut(s) 161, 300, 392, 1498
PleI GAGTC 3 cut(s) 205, 280, 1333
PpsI GAGTC 3 cut(s) 205, 280, 1333
PshAI GACNNNNGTC 1 cut(s) 215
Psp6I CCWGG 4 cut(s) 395, 719, 1253, 1316
PspEI GGTNACC 1 cut(s) 883
PspGI CCWGG 4 cut(s) 395, 719, 1253, 1316
PspN4I GGNNCC 4 cut(s) 36, 663, 853, 1386
PspPI GGNCC 2 cut(s) 35, 618
PspXI VCTCGAGB 1 cut(s) 1031
PstNI CAGNNNCTG 1 cut(s) 1235
PsuI RGATCY 3 cut(s) 379, 589, 1531
RsaI GTAC 5 cut(s) 107, 560, 821, 830, 1377
RsaNI GTAC 5 cut(s) 106, 559, 820, 829, 1376
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 5 cut(s) 495, 609, 686, 1460, 1512
SatI GCNGC 4 cut(s) 160, 299, 391, 1497
Sau3AI GATC 6 cut(s) 72, 379, 589, 1173, 1288, 1531
Sau96I GGNCC 2 cut(s) 35, 618
SchI GAGTC 3 cut(s) 205, 281, 1333
ScrFI CCNGG 5 cut(s) 397, 651, 721, 1255, 1318
SduI GDGCHC 3 cut(s) 66, 602, 1387
SfaNI GCATC 2 cut(s) 489, 1028
Sfr274I CTCGAG 1 cut(s) 1031
SinI GGWCC 1 cut(s) 618
SlaI CTCGAG 1 cut(s) 1031
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 3 cut(s) 332, 593, 1031
SmoI CTYRAG 3 cut(s) 332, 593, 1031
Sse9I AATT 6 cut(s) 761, 835, 841, 1045, 1211, 1508
SsiI CCGC 4 cut(s) 428, 782, 881, 1485
SspMI CTAG 3 cut(s) 351, 474, 1404
StyD4I CCNGG 5 cut(s) 395, 649, 719, 1253, 1316
StyI CCWWGG 2 cut(s) 151, 473
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 2 cut(s) 219, 834
TaqI TCGA 4 cut(s) 189, 845, 1032, 1207
TaqII GACCGA 1 cut(s) 635
TasI AATT 6 cut(s) 761, 835, 841, 1045, 1211, 1508
TatI WGTACW 3 cut(s) 105, 558, 1375
TfiI GAWTC 3 cut(s) 191, 400, 1115
Tru1I TTAA 5 cut(s) 495, 609, 686, 1460, 1512
Tru9I TTAA 5 cut(s) 495, 609, 686, 1460, 1512
TscAI CASTG 3 cut(s) 1236, 1254, 1371
TseI GCWGC 4 cut(s) 159, 298, 390, 1496
TspDTI ATGAA 3 cut(s) 223, 306, 536
TspRI CASTG 3 cut(s) 1236, 1254, 1371
Van91I CCANNNNNTGG 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 618
XagI CCTNNNNNAGG 1 cut(s) 235
XapI RAATTY 3 cut(s) 1045, 1211, 1508
XhoI CTCGAG 1 cut(s) 1031
XmaJI CCTAGG 1 cut(s) 473
XspI CTAG 3 cut(s) 351, 474, 1404
Zsp2I ATGCAT 1 cut(s) 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.