MD12G1189000.v1.1

Transmembrane protein 87B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003014
Physical Location & Seq
Reverse (-)
27067748 .. 27070835
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1189000.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGGAACAGAGAAGGTTCTGGTGTGAAAAGGGCTCGCTCTTAGGGTTAATTTTGGGGGTTTTTCTCACATGCAATGAGTGCATCATAAGGTCTGATGCTTCAATCCACGAGTACAGAAATGAAGCTTTCAATCCTCAGTCAAACGCCTTCTTCTTCCATGGCGGCAGTGAAGGCCTCTACGCTTCCAAGGTCCACGATTCTACTGATAATTCTTCAGCAGACAATCGCCACCTCAAGGGCAAGTCCTTCATCAAGTTTGAGAGTGTCACTTTTGTGAGGACGAAAGAGTCTGCGAATAAGATGAGTGAAATGCAGCAGGACACTGGATTGGTGGAAGCTATCATTCTTGAAGTGAAGGACAGGGCAAGGATTGGAGCTTCTTTCGTCCAATCGGATCAAATATGCTGCACCAAGAATCTATCGAATTCTGGATACTGCACGGCGGGAGAGGTTGTTATCCACAAAAATCTGGACAACCCGGAGTGGCCTGTGCGCATCAAAACATTCTTTAATGGAAACGATGAAGAGGCGAAGATGGAAATTAAGACGGTTGATATCAACAGTTCCGGAATGTACTACCTTTATTTCATGTACTGTGATCCGCAACTCAAGGGTACGCTGATTAAAGGAACGACCATCTGGAGAAACCCAGATGGTTATTTGCCTGGGAAGATGACTCCTTTGATGACATTCTATGGCTTTATGTCTTTAGCTTACCTTGTGCTTGGACTTGCCTGGTTTCTGAGATTTGTTCAGTTTTGGAAGGATATTATACAATTGCACTACCATATCACAGCGGTGATTGCTCTTGGAATGTGCGAAATGGCTGTGTGGTACTTTGAGTATGCCAATTTTAATTCAACTGGGATCAGACCTATGGGCATTACATTGTGGGCTGTAACTTTTAGTGCTGTCAAGAAGACTCTTTCCCGCCTTCTTCTTTTGGTCGTTTCCATGGGTTTTGGTGTAGTGAAGCCTACACTTGGTGGTATAACTCTGAAAGTATTTCTTCTTGGTCTGGTATATTTCGTGGCATCAGAAGCACTTGAGCTTGTTGAACATTTGGGGAACATCAATGATTTTTCAGGAAAAACAAAGTTATTTTTGGTTCTACCTGTTGCCTTCTTAGATTCGTGGTTTATTCTATGGATTTTCTCATCGTTATCAAAAACTTTAGAGAAACTTCAGATCAGGAGAAACTTGGCTAAATTGGAGCTATACCGAAAATTTACCAACTCGCTTGCAATATTTGTGCTTCTTTCGGTTGCTTGGATTGGGTTTGAGCTATACTTCAACGCAACAGATCCCCTGAGTGAGTTTTGGCAAATTGCTTGGATTATTCCAGCATTCTGGAGTATTCTTGCCTATGCTCTCTTGGCAATAATATGCATCCTTTGGGCCCCTTCACGTAACCCAACTAGATATGCATACATGGAGGAAGCAGGAGATGATTTTGATGAGGAGGGCATCTCGTTAACCAGTGGAGCGTTGAAGGTCAGTGGAGATGTGGCAATCAAGCGAGAATACGAGAATGCGCATGCAGATGATATGGAGGAGGATAAGCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0008150 GO:0016192 GO:0016197 GO:0016482 GO:0042147 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lung_7-TM_R

Protein Analysis

524

Amino Acids

59.45

Weight (kDa)

5.83

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 227 - 472 9.9e-93 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
Acc16I TGCGCA 2 cut(s) 494, 1536
AccIII TCCGGA 1 cut(s) 566
AciI CCGC 5 cut(s) 162, 443, 602, 797, 931
AclWI GGATC 4 cut(s) 402, 593, 875, 1298
AcsI RAATTY 2 cut(s) 424, 1226
AcuI CTGAAG 2 cut(s) 198, 1169
AdeI CACNNNGTG 1 cut(s) 986
AfaI GTAC 5 cut(s) 113, 575, 593, 616, 836
AfiI CCNNNNNNNGG 2 cut(s) 235, 983
AgsI TTSAA 7 cut(s) 102, 130, 350, 861, 1058, 1294, 1492
AjnI CCWGG 2 cut(s) 664, 734
AleI CACNNNNGTG 2 cut(s) 272, 797
AluBI AGCT 7 cut(s) 125, 338, 377, 713, 1051, 1216, 1285
AluI AGCT 7 cut(s) 125, 338, 377, 713, 1051, 1216, 1285
AlwI GGATC 4 cut(s) 402, 593, 875, 1298
Aor13HI TCCGGA 1 cut(s) 566
AoxI GGCC 3 cut(s) 172, 485, 1398
ApaI GGGCCC 1 cut(s) 1402
ApeKI GCWGC 2 cut(s) 313, 405
ApoI RAATTY 2 cut(s) 424, 1226
AspLEI GCGC 2 cut(s) 495, 1537
AspS9I GGNCC 3 cut(s) 190, 1398, 1399
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 1 cut(s) 811
AvaII GGWCC 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 1402
BanII GRGCYC 2 cut(s) 35, 1402
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 926
BbvI GCAGC 2 cut(s) 325, 392
BccI CCATC 3 cut(s) 529, 644, 647
BceAI ACGGC 1 cut(s) 456
BciT130I CCWGG 2 cut(s) 666, 736
BciVI GTATCC 1 cut(s) 425
BcnI CCSGG 1 cut(s) 479
BfaI CTAG 1 cut(s) 1419
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 3 cut(s) 163, 314, 406
BlsI GCNGC 3 cut(s) 164, 315, 407
Bme1390I CCNGG 3 cut(s) 479, 666, 736
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 190, 1398, 1399
BmiI GGNNCC 2 cut(s) 1400, 1401
BmrFI CCNGG 3 cut(s) 479, 666, 736
BmrI ACTGGG 1 cut(s) 873
BmsI GCATC 6 cut(s) 85, 90, 504, 1043, 1398, 1476
BmuI ACTGGG 1 cut(s) 873
BpiI GAAGAC 1 cut(s) 926
BplI GAGNNNNNCTC 2 cut(s) 1454, 1486
BpmI CTGGAG 2 cut(s) 661, 1372
BpuEI CTTGAG 3 cut(s) 218, 593, 1067
BpuMI CCSGG 1 cut(s) 479
BsaAI YACGTR 1 cut(s) 1409
BsaJI CCNNGG 4 cut(s) 157, 186, 665, 954
BsaWI WCCGGW 1 cut(s) 566
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 983
Bse1I ACTGG 3 cut(s) 328, 868, 1479
Bse3DI GCAATG 1 cut(s) 79
BseAI TCCGGA 1 cut(s) 566
BseBI CCWGG 2 cut(s) 666, 736
BseDI CCNNGG 4 cut(s) 157, 186, 665, 954
BseGI GGATG 1 cut(s) 1389
BseLI CCNNNNNNNGG 2 cut(s) 235, 983
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 3 cut(s) 149, 734, 1301
BseNI ACTGG 3 cut(s) 328, 868, 1479
BseRI GAGGAG 2 cut(s) 1475, 1568
BseSI GKGCMC 1 cut(s) 1402
BseXI GCAGC 2 cut(s) 325, 392
BsgI GTGCAG 2 cut(s) 391, 421
BshFI GGCC 3 cut(s) 174, 487, 1400
BsiSI CCGG 2 cut(s) 479, 567
BslI CCNNNNNNNGG 2 cut(s) 235, 983
BsmI GAATGC 2 cut(s) 1346, 1537
BsnI GGCC 3 cut(s) 174, 487, 1400
Bsp120I GGGCCC 1 cut(s) 1398
Bsp1286I GDGCHC 2 cut(s) 35, 1402
Bsp13I TCCGGA 1 cut(s) 566
Bsp143I GATC 5 cut(s) 394, 598, 867, 1188, 1303
Bsp19I CCATGG 2 cut(s) 157, 954
BspACI CCGC 5 cut(s) 162, 443, 602, 797, 931
BspANI GGCC 3 cut(s) 174, 487, 1400
BspCNI CTCAG 3 cut(s) 148, 735, 1302
BspEI TCCGGA 1 cut(s) 566
BspLI GGNNCC 2 cut(s) 1400, 1401
BspPI GGATC 4 cut(s) 402, 593, 875, 1298
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 3 cut(s) 328, 868, 1479
BssECI CCNNGG 4 cut(s) 157, 186, 665, 954
BssMI GATC 5 cut(s) 394, 598, 867, 1188, 1303
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 3 cut(s) 157, 186, 954
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 666, 736
Bst4CI ACNGT 3 cut(s) 550, 563, 596
Bst6I CTCTTC 1 cut(s) 519
BstAPI GCANNNNNTGC 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 1409
BstC8I GCNNGC 3 cut(s) 35, 1242, 1539
BstDEI CTNAG 5 cut(s) 40, 135, 743, 1126, 1310
BstDSI CCRYGG 2 cut(s) 157, 954
BstF5I GGATG 1 cut(s) 1389
BstHHI GCGC 2 cut(s) 495, 1537
BstKTI GATC 5 cut(s) 397, 601, 870, 1191, 1306
BstMBI GATC 5 cut(s) 394, 598, 867, 1188, 1303
BstMWI GCNNNNNNNGC 5 cut(s) 78, 171, 803, 1040, 1376
BstNI CCWGG 2 cut(s) 666, 736
BstNSI RCATGY 2 cut(s) 72, 1541
BstSCI CCNGG 3 cut(s) 477, 664, 734
BstSLI GKGCMC 1 cut(s) 1402
BstV1I GCAGC 2 cut(s) 325, 392
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 1 cut(s) 1303
BstXI CCANNNNNNTGG 1 cut(s) 1350
BstYI RGATCY 1 cut(s) 1303
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 3 cut(s) 174, 487, 1400
BtgI CCRYGG 2 cut(s) 157, 954
BtsCI GGATG 1 cut(s) 1389
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 4 cut(s) 172, 321, 1486, 1504
Cac8I GCNNGC 3 cut(s) 35, 1242, 1539
CfoI GCGC 2 cut(s) 495, 1537
Cfr13I GGNCC 3 cut(s) 190, 1398, 1399
Csp6I GTAC 5 cut(s) 112, 574, 592, 615, 835
CviAII CATG 6 cut(s) 69, 158, 589, 955, 1432, 1538
CviQI GTAC 5 cut(s) 112, 574, 592, 615, 835
DdeI CTNAG 5 cut(s) 40, 135, 743, 1126, 1310
DpnI GATC 5 cut(s) 396, 600, 869, 1190, 1305
DpnII GATC 5 cut(s) 394, 598, 867, 1188, 1303
DraIII CACNNNGTG 1 cut(s) 986
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco130I CCWWGG 3 cut(s) 157, 186, 954
Eco147I AGGCCT 1 cut(s) 174
Eco24I GRGCYC 2 cut(s) 35, 1402
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 198, 1169
EcoO109I RGGNCCY 1 cut(s) 1399
EcoRI GAATTC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 664, 734
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 3 cut(s) 157, 186, 954
EcoT22I ATGCAT 2 cut(s) 1391, 1429
EcoT38I GRGCYC 2 cut(s) 35, 1402
ErhI CCWWGG 3 cut(s) 157, 186, 954
FaeI CATG 6 cut(s) 72, 161, 592, 958, 1435, 1541
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FatI CATG 6 cut(s) 68, 157, 588, 954, 1431, 1537
FauI CCCGC 2 cut(s) 436, 938
Fnu4HI GCNGC 3 cut(s) 163, 314, 406
FokI GGATG 1 cut(s) 1376
FriOI GRGCYC 2 cut(s) 35, 1402
Fsp4HI GCNGC 3 cut(s) 163, 314, 406
FspAI RTGCGCAY 2 cut(s) 494, 1536
FspBI CTAG 1 cut(s) 1419
FspI TGCGCA 2 cut(s) 494, 1536
GlaI GCGC 2 cut(s) 494, 1536
GluI GCNGC 3 cut(s) 163, 314, 406
GsuI CTGGAG 2 cut(s) 661, 1372
HaeIII GGCC 3 cut(s) 174, 487, 1400
HapII CCGG 2 cut(s) 479, 567
HhaI GCGC 2 cut(s) 495, 1537
Hin1II CATG 6 cut(s) 72, 161, 592, 958, 1435, 1541
Hin6I GCGC 2 cut(s) 493, 1535
HinP1I GCGC 2 cut(s) 493, 1535
HincII GTYRAC 1 cut(s) 1476
HindII GTYRAC 1 cut(s) 1476
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 6 cut(s) 197, 287, 415, 676, 922, 1130
HpaI GTTAAC 1 cut(s) 1476
HpaII CCGG 2 cut(s) 479, 567
HphI GGTGA 1 cut(s) 811
Hpy166II GTNNAC 2 cut(s) 193, 1476
Hpy188I TCNGA 7 cut(s) 94, 394, 744, 872, 999, 1039, 1188
Hpy188III TCNNGA 9 cut(s) 347, 429, 470, 567, 640, 916, 1086, 1192, 1351
Hpy8I GTNNAC 2 cut(s) 193, 1476
HpyCH4III ACNGT 3 cut(s) 550, 563, 596
HpyCH4IV ACGT 1 cut(s) 1408
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 171, 803, 1040, 1376
HpyF3I CTNAG 5 cut(s) 40, 135, 743, 1126, 1310
HpySE526I ACGT 1 cut(s) 1408
Hsp92II CATG 6 cut(s) 72, 161, 592, 958, 1435, 1541
HspAI GCGC 2 cut(s) 493, 1535
Kpn2I TCCGGA 1 cut(s) 566
KspAI GTTAAC 1 cut(s) 1476
Kzo9I GATC 5 cut(s) 394, 598, 867, 1188, 1303
LmnI GCTCC 3 cut(s) 374, 1213, 1484
Lsp1109I GCAGC 2 cut(s) 325, 392
LweI GCATC 6 cut(s) 85, 90, 504, 1043, 1398, 1476
MaeI CTAG 1 cut(s) 1419
MaeII ACGT 1 cut(s) 1408
MaeIII GTNAC 3 cut(s) 265, 898, 1409
MalI GATC 5 cut(s) 396, 600, 869, 1190, 1305
MboI GATC 5 cut(s) 394, 598, 867, 1188, 1303
MboII GAAGA 9 cut(s) 142, 145, 204, 536, 544, 682, 929, 931, 1001
MfeI CAATTG 1 cut(s) 776
MflI RGATCY 1 cut(s) 1303
MhlI GDGCHC 2 cut(s) 35, 1402
MlyI GAGTC 3 cut(s) 296, 670, 916
Mph1103I ATGCAT 2 cut(s) 1391, 1429
MroI TCCGGA 1 cut(s) 566
MseI TTAA 6 cut(s) 47, 510, 543, 624, 855, 1475
MslI CAYNNNNRTG 3 cut(s) 272, 797, 1542
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 2 cut(s) 479, 567
MspR9I CCNGG 3 cut(s) 479, 666, 736
MunI CAATTG 1 cut(s) 776
Mva1269I GAATGC 2 cut(s) 1346, 1537
MvaI CCWGG 2 cut(s) 666, 736
MwoI GCNNNNNNNGC 5 cut(s) 78, 171, 803, 1040, 1376
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 2 cut(s) 157, 954
NdeII GATC 5 cut(s) 394, 598, 867, 1188, 1303
NlaIII CATG 6 cut(s) 72, 161, 592, 958, 1435, 1541
NlaIV GGNNCC 2 cut(s) 1400, 1401
NmuCI GTSAC 1 cut(s) 265
NsbI TGCGCA 2 cut(s) 494, 1536
NsiI ATGCAT 2 cut(s) 1391, 1429
NspI RCATGY 2 cut(s) 72, 1541
OliI CACNNNNGTG 2 cut(s) 272, 797
PaeI GCATGC 1 cut(s) 1541
PceI AGGCCT 1 cut(s) 174
PctI GAATGC 2 cut(s) 1346, 1537
PfeI GAWTC 3 cut(s) 197, 415, 1130
PkrI GCNGC 3 cut(s) 164, 315, 407
PleI GAGTC 3 cut(s) 295, 670, 916
PpsI GAGTC 3 cut(s) 295, 670, 916
Ppu21I YACGTR 1 cut(s) 1409
Psp6I CCWGG 2 cut(s) 664, 734
PspGI CCWGG 2 cut(s) 664, 734
PspN4I GGNNCC 2 cut(s) 1400, 1401
PspOMI GGGCCC 1 cut(s) 1398
PspPI GGNCC 3 cut(s) 190, 1398, 1399
PsuI RGATCY 1 cut(s) 1303
RsaI GTAC 5 cut(s) 113, 575, 593, 616, 836
RsaNI GTAC 5 cut(s) 112, 574, 592, 615, 835
RseI CAYNNNNRTG 3 cut(s) 272, 797, 1542
SaqAI TTAA 6 cut(s) 47, 510, 543, 624, 855, 1475
SatI GCNGC 3 cut(s) 163, 314, 406
Sau3AI GATC 5 cut(s) 394, 598, 867, 1188, 1303
Sau96I GGNCC 3 cut(s) 190, 1398, 1399
SchI GAGTC 3 cut(s) 296, 670, 916
ScrFI CCNGG 3 cut(s) 479, 666, 736
SduI GDGCHC 2 cut(s) 35, 1402
SfaNI GCATC 6 cut(s) 85, 90, 504, 1043, 1398, 1476
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 3 cut(s) 272, 797, 1542
SmlI CTYRAG 3 cut(s) 233, 608, 1046
SmoI CTYRAG 3 cut(s) 233, 608, 1046
SphI GCATGC 1 cut(s) 1541
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 5 cut(s) 162, 443, 602, 797, 931
SspI AATATT 1 cut(s) 1248
SspMI CTAG 1 cut(s) 1419
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 477, 664, 734
StyI CCWWGG 3 cut(s) 157, 186, 954
TaaI ACNGT 3 cut(s) 550, 563, 596
TaiI ACGT 1 cut(s) 1411
TaqI TCGA 1 cut(s) 422
TatI WGTACW 3 cut(s) 111, 573, 591
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 3 cut(s) 197, 415, 1130
Tru1I TTAA 6 cut(s) 47, 510, 543, 624, 855, 1475
Tru9I TTAA 6 cut(s) 47, 510, 543, 624, 855, 1475
TscAI CASTG 4 cut(s) 172, 328, 1486, 1504
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 313, 405
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 4 cut(s) 135, 238, 537, 577
TspRI CASTG 4 cut(s) 172, 328, 1486, 1504
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 424, 1226
XceI RCATGY 2 cut(s) 72, 1541
XspI CTAG 1 cut(s) 1419
Zsp2I ATGCAT 2 cut(s) 1391, 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.