MD07G1310100.v1.1

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004544
Physical Location & Seq
Forward (+)
36498105 .. 36501540
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1310100.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGATGAGAATCCTCAACTTGCGGTTCATTGAAGGCTCATTCAGTTCAACATCCCTACCTTTCTTCCTCTCCTCCCAACGCGTGCGGGCACGGTCGGCAGGGACGGAGGCAGGGACAGGGACCGGGACAGGGAGGGTCCTCGATTTCTCGTGTGGTCTTAACATGGCTTTGGCCGGCAAAGGTGTAATTGTCGACCACCAAGCCTTTCATAATTTGACCTCCTCTCAACTTCAACACAAGGGTGCAACAATTCCAGAATCATTGTCAGGACTTCCACTTCTTGTTAGAGGACGTTTCCATGGAATAGGAGCAGCAGCAGGAGGAGGAGGAGCTTCCCAAATTTCCAAGCCACAGTTCACTAAGCTTTTGAAACATGTCACAAGTCATATATCATCCATCTCCAACATTTTTGTGCATGATGGTGCTATCGGTTCATTACCAAAATGTGATGCAAAAGTTCGTTTAATAAGTGATAGTTCGTCTGCTGTGCTCTCACTATCCCGTATTCTCTGGGGGACTTCCACCCGTGCAGTTTCTCATGATTCATGTCCTTTGACAGTTTATGTGGCTACTTCTATAAGTTCAGGAATTGGGGATGTTATTGACCTTGGATCTAAAGGAAATAATGGATTTATAGCTGCTGATATTGAGCGTTCATCACTTGTTTTATGTGGTAAAGCCTTCTCAGATTTAAATGTAACTAAAGAAGCACTAGCGGCCTTGTCTGGACCTGTTATATTTGCACGGGGAGGCCTTCCACTATGTGCAAGGCTTCTAGTGTCTGGTGATTCTGTGGTTCTTATATTTGCACCCAAAGGTACCTTTAAGAGCTGCAGACATCTGTTGGTGCCTAAGGAGGCAGGTGTAATTCTTTCATCTGAAGGTGTTGCACCATTGTTTCAAACTGGTGGCCAAAATCTGTTCAAGTTCCCATCTGTTGTAGTCCTTGCATCTTCTGACAGTTCTGGCGTTATCCCTTCTGTTTCAAAGCTCTCCCCTGGCCAGGCAGCTTATCACTTCTTAGCTGGTTATCAGAATGGGAAGTTCATGCCCGCATACAACAAAGGCCCTTCGTATATTCACCCAATGGAACTAGCTAAGGCATTTCTGTCCAAGTTGCAAGATGACAAGATATCATCTTACTTATTGAATGTCAATGGAGGAGAAAAGAATGTAAATGGCAAAGATTTGGCTAATCTGGTGCAATCAACTTTGTCCGAGAATATCCCACCATTTGAAGCTAAAAGTGGGGATCTTGAAGACAAGTACAAGAGCTTCCTGTTGAGCGAATTTCAAGAAGTGCCAGAAGAATTATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

441

Amino Acids

46.32

Weight (kDa)

9.01

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 309 - 384 5.4e-08 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 818
AccB1I GGYRCC 2 cut(s) 818, 847
AccI GTMKAC 1 cut(s) 192
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 22, 85, 716, 1053
AclWI GGATC 2 cut(s) 619, 1260
AcoI YGGCCR 3 cut(s) 171, 910, 1000
AcsI RAATTY 2 cut(s) 339, 1289
AcuI CTGAAG 1 cut(s) 900
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 2 cut(s) 820, 1268
AfiI CCNNNNNNNGG 2 cut(s) 129, 1003
AflIII ACRYGT 2 cut(s) 79, 373
AjnI CCWGG 2 cut(s) 997, 1002
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AleI CACNNNNGTG 1 cut(s) 240
Alw21I GWGCWC 1 cut(s) 492
AlwI GGATC 2 cut(s) 619, 1260
AoxI GGCC 6 cut(s) 171, 717, 751, 910, 1000, 1066
ApeKI GCWGC 5 cut(s) 311, 314, 638, 831, 1007
ApoI RAATTY 2 cut(s) 339, 1289
Asp718I GGTACC 1 cut(s) 818
AspS9I GGNCC 4 cut(s) 120, 136, 728, 1067
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 797, 1073
AvaII GGWCC 3 cut(s) 120, 136, 728
AxyI CCTNAGG 1 cut(s) 852
BaeGI GKGCMC 1 cut(s) 91
BalI TGGCCA 2 cut(s) 912, 1002
BanI GGYRCC 2 cut(s) 818, 847
BarI GAAGNNNNNNTAC 2 cut(s) 1259, 1291
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 1266
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 5 cut(s) 323, 326, 625, 818, 1019
BccI CCATC 3 cut(s) 404, 413, 940
BciT130I CCWGG 2 cut(s) 999, 1004
BcnI CCSGG 1 cut(s) 124
BfaI CTAG 3 cut(s) 713, 776, 1094
BfmI CTRYAG 1 cut(s) 832
BfuAI ACCTGC 1 cut(s) 851
BisI GCNGC 6 cut(s) 312, 315, 639, 717, 832, 1008
BlsI GCNGC 6 cut(s) 313, 316, 640, 718, 833, 1009
Bme1390I CCNGG 3 cut(s) 124, 999, 1004
Bme18I GGWCC 3 cut(s) 120, 136, 728
BmgT120I GGNCC 4 cut(s) 120, 136, 728, 1067
BmiI GGNNCC 4 cut(s) 121, 137, 820, 849
BmrFI CCNGG 3 cut(s) 124, 999, 1004
BmsI GCATC 2 cut(s) 439, 959
BpiI GAAGAC 1 cut(s) 1266
Bpu10I CCTNAGC 1 cut(s) 1098
BpuMI CCSGG 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 298, 607, 997
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 1003
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 910
Bse21I CCTNAGG 1 cut(s) 852
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 2 cut(s) 999, 1004
BseDI CCNNGG 3 cut(s) 298, 607, 997
BseGI GGATG 3 cut(s) 50, 392, 601
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 2 cut(s) 129, 1003
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 6 cut(s) 61, 211, 336, 339, 342, 1176
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 5 cut(s) 323, 326, 625, 818, 1019
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 6 cut(s) 173, 719, 753, 912, 1002, 1068
BshNI GGYRCC 2 cut(s) 818, 847
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 2 cut(s) 123, 174
BslFI GGGAC 5 cut(s) 115, 127, 133, 139, 529
BslI CCNNNNNNNGG 2 cut(s) 129, 1003
BsmFI GGGAC 5 cut(s) 115, 127, 133, 139, 529
BsnI GGCC 6 cut(s) 173, 719, 753, 912, 1002, 1068
Bsp1286I GDGCHC 2 cut(s) 91, 492
Bsp143I GATC 2 cut(s) 611, 1252
Bsp19I CCATGG 1 cut(s) 298
BspACI CCGC 4 cut(s) 22, 85, 716, 1053
BspANI GGCC 6 cut(s) 173, 719, 753, 912, 1002, 1068
BspCNI CTCAG 1 cut(s) 698
BspFNI CGCG 1 cut(s) 81
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 4 cut(s) 121, 137, 820, 849
BspMAI CTGCAG 1 cut(s) 836
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 2 cut(s) 619, 1260
BspT107I GGYRCC 2 cut(s) 818, 847
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 910
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 298, 607, 997
BssMI GATC 2 cut(s) 611, 1252
BssSI CACGAG 1 cut(s) 148
BssT1I CCWWGG 2 cut(s) 298, 607
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 999, 1004
Bst4CI ACNGT 4 cut(s) 93, 354, 559, 962
BstC8I GCNNGC 4 cut(s) 83, 87, 175, 1053
BstDEI CTNAG 5 cut(s) 360, 685, 852, 1021, 1098
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 50, 392, 601
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 2 cut(s) 614, 1255
BstMBI GATC 2 cut(s) 611, 1252
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 95, 716
BstNI CCWGG 2 cut(s) 999, 1004
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 3 cut(s) 122, 997, 1002
BstSFI CTRYAG 1 cut(s) 832
BstSLI GKGCMC 1 cut(s) 91
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 5 cut(s) 323, 326, 625, 818, 1019
BstV2I GAAGAC 1 cut(s) 1266
BstX2I RGATCY 2 cut(s) 611, 1252
BstYI RGATCY 2 cut(s) 611, 1252
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 6 cut(s) 173, 719, 753, 912, 1002, 1068
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 3 cut(s) 50, 392, 601
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 4 cut(s) 83, 87, 175, 1053
CciI TCATGA 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 4 cut(s) 120, 136, 728, 1067
Csp6I GTAC 2 cut(s) 819, 1267
CviAII CATG 7 cut(s) 163, 299, 374, 416, 539, 546, 1048
CviQI GTAC 2 cut(s) 819, 1267
DdeI CTNAG 5 cut(s) 360, 685, 852, 1021, 1098
DpnI GATC 2 cut(s) 613, 1254
DpnII GATC 2 cut(s) 611, 1252
DraI TTTAAA 1 cut(s) 693
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 3 cut(s) 171, 910, 1000
Eco130I CCWWGG 2 cut(s) 298, 607
Eco147I AGGCCT 1 cut(s) 753
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 3 cut(s) 120, 136, 728
Eco57I CTGAAG 1 cut(s) 900
Eco81I CCTNAGG 1 cut(s) 852
EcoO109I RGGNCCY 2 cut(s) 136, 1067
EcoRII CCWGG 2 cut(s) 997, 1002
EcoRV GATATC 1 cut(s) 1134
EcoT14I CCWWGG 2 cut(s) 298, 607
ErhI CCWWGG 2 cut(s) 298, 607
FaeI CATG 7 cut(s) 166, 302, 377, 419, 542, 549, 1051
FaqI GGGAC 5 cut(s) 115, 127, 133, 139, 529
FatI CATG 7 cut(s) 162, 298, 373, 415, 538, 545, 1047
FauI CCCGC 2 cut(s) 78, 1060
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 6 cut(s) 312, 315, 639, 717, 832, 1008
FokI GGATG 3 cut(s) 37, 379, 608
Fsp4HI GCNGC 6 cut(s) 312, 315, 639, 717, 832, 1008
FspBI CTAG 3 cut(s) 713, 776, 1094
GluI GCNGC 6 cut(s) 312, 315, 639, 717, 832, 1008
HaeIII GGCC 6 cut(s) 173, 719, 753, 912, 1002, 1068
HapII CCGG 2 cut(s) 123, 174
Hin1II CATG 7 cut(s) 166, 302, 377, 419, 542, 549, 1051
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 4 cut(s) 9, 257, 542, 788
HpaII CCGG 2 cut(s) 123, 174
HphI GGTGA 2 cut(s) 797, 1073
Hpy166II GTNNAC 2 cut(s) 193, 357
Hpy188I TCNGA 5 cut(s) 688, 880, 958, 1035, 1219
Hpy188III TCNNGA 7 cut(s) 254, 267, 539, 585, 726, 1256, 1295
Hpy8I GTNNAC 2 cut(s) 193, 357
HpyAV CCTTC 6 cut(s) 26, 691, 764, 875, 987, 1080
HpyCH4III ACNGT 4 cut(s) 93, 354, 559, 962
HpyCH4IV ACGT 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 716
HpyF3I CTNAG 5 cut(s) 360, 685, 852, 1021, 1098
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 7 cut(s) 166, 302, 377, 419, 542, 549, 1051
KpnI GGTACC 1 cut(s) 822
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 611, 1252
LmnI GCTCC 2 cut(s) 308, 329
Lsp1109I GCAGC 5 cut(s) 323, 326, 625, 818, 1019
LweI GCATC 2 cut(s) 439, 959
MaeI CTAG 3 cut(s) 713, 776, 1094
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 376, 697
MalI GATC 2 cut(s) 613, 1254
MboI GATC 2 cut(s) 611, 1252
MboII GAAGA 4 cut(s) 55, 945, 1271, 1319
MflI RGATCY 2 cut(s) 611, 1252
MhlI GDGCHC 2 cut(s) 91, 492
MlsI TGGCCA 2 cut(s) 912, 1002
MluCI AATT 8 cut(s) 186, 211, 249, 339, 588, 867, 1289, 1310
MluI ACGCGT 1 cut(s) 79
MluNI TGGCCA 2 cut(s) 912, 1002
MmeI TCCRAC 1 cut(s) 426
Mox20I TGGCCA 2 cut(s) 912, 1002
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 2 cut(s) 912, 1002
MseI TTAA 4 cut(s) 159, 464, 692, 825
MslI CAYNNNNRTG 3 cut(s) 240, 410, 420
Msp20I TGGCCA 2 cut(s) 912, 1002
MspI CCGG 2 cut(s) 123, 174
MspR9I CCNGG 3 cut(s) 124, 999, 1004
MvaI CCWGG 2 cut(s) 999, 1004
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 95, 716
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 298
NdeII GATC 2 cut(s) 611, 1252
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 7 cut(s) 166, 302, 377, 419, 542, 549, 1051
NlaIV GGNNCC 4 cut(s) 121, 137, 820, 849
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 1 cut(s) 240
PagI TCATGA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 851
PceI AGGCCT 1 cut(s) 753
PciI ACATGT 1 cut(s) 373
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 4 cut(s) 9, 257, 542, 788
PkrI GCNGC 6 cut(s) 313, 316, 640, 718, 833, 1009
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 997, 1002
PspGI CCWGG 2 cut(s) 997, 1002
PspN4I GGNNCC 4 cut(s) 121, 137, 820, 849
PspPI GGNCC 4 cut(s) 120, 136, 728, 1067
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 836
PsuI RGATCY 2 cut(s) 611, 1252
RsaI GTAC 2 cut(s) 820, 1268
RsaNI GTAC 2 cut(s) 819, 1267
RseI CAYNNNNRTG 3 cut(s) 240, 410, 420
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 4 cut(s) 159, 464, 692, 825
SatI GCNGC 6 cut(s) 312, 315, 639, 717, 832, 1008
Sau3AI GATC 2 cut(s) 611, 1252
Sau96I GGNCC 4 cut(s) 120, 136, 728, 1067
ScrFI CCNGG 3 cut(s) 124, 999, 1004
SduI GDGCHC 2 cut(s) 91, 492
SfaNI GCATC 2 cut(s) 439, 959
SfcI CTRYAG 1 cut(s) 832
SinI GGWCC 3 cut(s) 120, 136, 728
SmiI ATTTAAAT 1 cut(s) 693
SmiMI CAYNNNNRTG 3 cut(s) 240, 410, 420
Sse9I AATT 8 cut(s) 186, 211, 249, 339, 588, 867, 1289, 1310
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 4 cut(s) 22, 85, 716, 1053
SspMI CTAG 3 cut(s) 713, 776, 1094
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 3 cut(s) 122, 997, 1002
StyI CCWWGG 2 cut(s) 298, 607
SwaI ATTTAAAT 1 cut(s) 693
TaaI ACNGT 4 cut(s) 93, 354, 559, 962
TaiI ACGT 1 cut(s) 295
TaqI TCGA 2 cut(s) 141, 192
TasI AATT 8 cut(s) 186, 211, 249, 339, 588, 867, 1289, 1310
TatI WGTACW 1 cut(s) 1266
TauI GCSGC 1 cut(s) 719
TfiI GAWTC 4 cut(s) 9, 257, 542, 788
Tru1I TTAA 4 cut(s) 159, 464, 692, 825
Tru9I TTAA 4 cut(s) 159, 464, 692, 825
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 5 cut(s) 311, 314, 638, 831, 1007
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 7 cut(s) 16, 197, 423, 534, 645, 864, 1036
TspGWI ACGGA 1 cut(s) 119
VpaK11BI GGWCC 3 cut(s) 120, 136, 728
XapI RAATTY 2 cut(s) 339, 1289
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 3 cut(s) 713, 776, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.