Rroxscaffold_4G00276670

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004544
Physical Location & Seq
Reverse (-)
195763 .. 198705
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276670.1

Sequence Viewer

Length: 732 bp
ATGTCAAACATTAATGTCCATGATGGGGCAGTTGGTTCATCACTCAAAGCTGGTGCAAAAGTTCGCGTTATAAGTAATAGCCCATCTGCTGTATTGTCACTTTCCAGCATTCTCTGGGGGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTCTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTATTGGCCTTGAATTGAAAAGAGATAATGCACTTATAGCTGCTGATATTGAGCGTTCTTCACTTGTTTTATGTGGCAATGCCTTCTCAGATTCAAATGCAACTAAACAAGCACTAGCTGCCTTGTCCGGACCTGTTATATTTGCCCGAAGAGGCCTTCCCCTATCTGCAAGGCTTTTAGTGTTTGGTGTTGCCCCATTGTTTCAAACTGGAAGCTCTGGTAGCTCAAATCTATTCAAGTTGCCAGCTACTGTAGTCCTTGCAACTTTTGACAGTTCGGGTGTTATACCTTCTGTATCAAAGCTTGCACCTGGCCAGGCAGCTTATCACTTCCTGGCTGGTTATCAAAATGGGAAGTTCATGCCTGCATACAGCAAAGGCCCCTCATCTATTGACCTGTTGGAACTAGCAAAGGAGTTTCTGTTGAAGTTGAAAGATGACCGGATCTCATCCTACCTGGTTAATGCCAATGGAGGAGAAAAGGGTGCAACTGGTAAGAGCAAATCGGATGACTTGGACTTTCTCTTGGATAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

243

Amino Acids

25.42

Weight (kDa)

9.16

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 143 - 180 1.7e-08 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AccII CGCG 1 cut(s) 66
AccIII TCCGGA 1 cut(s) 320
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 7 cut(s) 206, 211, 288, 398, 430, 619, 625
AjnI CCWGG 4 cut(s) 502, 507, 525, 648
AluBI AGCT 8 cut(s) 50, 233, 311, 408, 417, 440, 496, 515
AluI AGCT 8 cut(s) 50, 233, 311, 408, 417, 440, 496, 515
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 1 cut(s) 644
AlwNI CAGNNNCTG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 320
AoxI GGCC 4 cut(s) 199, 346, 505, 571
ApeKI GCWGC 3 cut(s) 233, 311, 512
AseI ATTAAT 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 323, 572
AvaII GGWCC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 507
BbvI GCAGC 3 cut(s) 220, 298, 524
BccI CCATC 2 cut(s) 17, 91
BciT130I CCWGG 4 cut(s) 504, 509, 527, 650
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 2 cut(s) 308, 599
BfmI CTRYAG 1 cut(s) 444
BisI GCNGC 3 cut(s) 234, 312, 513
BlsI GCNGC 3 cut(s) 235, 313, 514
Bme1390I CCNGG 4 cut(s) 504, 509, 527, 650
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 2 cut(s) 323, 572
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 4 cut(s) 504, 509, 527, 650
BsaWI WCCGGW 2 cut(s) 320, 633
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 406, 688
Bse3DI GCAATG 1 cut(s) 277
BseAI TCCGGA 1 cut(s) 320
BseBI CCWGG 4 cut(s) 504, 509, 527, 650
BseGI GGATG 2 cut(s) 641, 706
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 2 cut(s) 406, 688
BseRI GAGGAG 1 cut(s) 681
BseXI GCAGC 3 cut(s) 220, 298, 524
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 201, 348, 507, 573
BsiSI CCGG 2 cut(s) 321, 634
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 4 cut(s) 201, 348, 507, 573
Bsp13I TCCGGA 1 cut(s) 320
Bsp143I GATC 1 cut(s) 636
BspANI GGCC 4 cut(s) 201, 348, 507, 573
BspCNI CTCAG 1 cut(s) 293
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 574
BspPI GGATC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 2 cut(s) 406, 688
BssMI GATC 1 cut(s) 636
Bst2UI CCWGG 4 cut(s) 504, 509, 527, 650
Bst4CI ACNGT 3 cut(s) 163, 445, 467
Bst6I CTCTTC 1 cut(s) 337
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 438, 498, 558
BstDEI CTNAG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 641, 706
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 1 cut(s) 639
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 1 cut(s) 636
BstMWI GCNNNNNNNGC 3 cut(s) 230, 311, 414
BstNI CCWGG 4 cut(s) 504, 509, 527, 650
BstSCI CCNGG 4 cut(s) 502, 507, 525, 648
BstSFI CTRYAG 1 cut(s) 444
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 220, 298, 524
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
BsuRI GGCC 4 cut(s) 201, 348, 507, 573
BtsCI GGATG 2 cut(s) 641, 706
Cac8I GCNNGC 3 cut(s) 438, 498, 558
CaiI CAGNNNCTG 1 cut(s) 443
CciI TCATGA 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 323, 572
CsiI ACCWGGT 1 cut(s) 648
CviAII CATG 3 cut(s) 20, 143, 553
DdeI CTNAG 1 cut(s) 280
DpnI GATC 1 cut(s) 638
DpnII GATC 1 cut(s) 636
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
Eco147I AGGCCT 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 572
EcoRII CCWGG 4 cut(s) 502, 507, 525, 648
FaeI CATG 3 cut(s) 23, 146, 556
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 19, 142, 552
Fnu4HI GCNGC 3 cut(s) 234, 312, 513
FokI GGATG 2 cut(s) 628, 713
Fsp4HI GCNGC 3 cut(s) 234, 312, 513
FspBI CTAG 2 cut(s) 308, 599
GluI GCNGC 3 cut(s) 234, 312, 513
HaeIII GGCC 4 cut(s) 201, 348, 507, 573
HapII CCGG 2 cut(s) 321, 634
Hin1II CATG 3 cut(s) 23, 146, 556
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 2 cut(s) 146, 284
HpaII CCGG 2 cut(s) 321, 634
Hpy188I TCNGA 2 cut(s) 283, 700
Hpy188III TCNNGA 3 cut(s) 143, 188, 321
HpyAV CCTTC 3 cut(s) 286, 359, 492
HpyCH4III ACNGT 3 cut(s) 163, 445, 467
HpyCH4V TGCA 9 cut(s) 56, 134, 224, 293, 362, 455, 500, 560, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 311, 414
HpyF3I CTNAG 1 cut(s) 280
Hsp92II CATG 3 cut(s) 23, 146, 556
Kpn2I TCCGGA 1 cut(s) 320
Kzo9I GATC 1 cut(s) 636
Lsp1109I GCAGC 3 cut(s) 220, 298, 524
MabI ACCWGGT 1 cut(s) 648
MaeI CTAG 2 cut(s) 308, 599
MaeIII GTNAC 1 cut(s) 96
MalI GATC 1 cut(s) 638
MboI GATC 1 cut(s) 636
MboII GAAGA 2 cut(s) 243, 354
MflI RGATCY 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 507
MmeI TCCRAC 1 cut(s) 573
MnlI CCTC 4 cut(s) 165, 338, 586, 659
Mox20I TGGCCA 1 cut(s) 507
MroI TCCGGA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 507
MseI TTAA 2 cut(s) 12, 654
Msp20I TGGCCA 1 cut(s) 507
MspI CCGG 2 cut(s) 321, 634
MspR9I CCNGG 4 cut(s) 504, 509, 527, 650
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 4 cut(s) 504, 509, 527, 650
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 230, 311, 414
NdeII GATC 1 cut(s) 636
NlaIII CATG 3 cut(s) 23, 146, 556
NlaIV GGNNCC 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 96
PagI TCATGA 1 cut(s) 142
PceI AGGCCT 1 cut(s) 348
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 146, 284
PkrI GCNGC 3 cut(s) 235, 313, 514
PshBI ATTAAT 1 cut(s) 12
PsiI TTATAA 1 cut(s) 71
Psp6I CCWGG 4 cut(s) 502, 507, 525, 648
PspGI CCWGG 4 cut(s) 502, 507, 525, 648
PspN4I GGNNCC 1 cut(s) 574
PspPI GGNCC 2 cut(s) 323, 572
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 1 cut(s) 636
SaqAI TTAA 2 cut(s) 12, 654
SatI GCNGC 3 cut(s) 234, 312, 513
Sau3AI GATC 1 cut(s) 636
Sau96I GGNCC 2 cut(s) 323, 572
ScrFI CCNGG 4 cut(s) 504, 509, 527, 650
SexAI ACCWGGT 1 cut(s) 648
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 323
Sse9I AATT 1 cut(s) 206
SseBI AGGCCT 1 cut(s) 348
SspMI CTAG 2 cut(s) 308, 599
StuI AGGCCT 1 cut(s) 348
StyD4I CCNGG 4 cut(s) 502, 507, 525, 648
TaaI ACNGT 3 cut(s) 163, 445, 467
TasI AATT 1 cut(s) 206
TfiI GAWTC 2 cut(s) 146, 284
Tru1I TTAA 2 cut(s) 12, 654
Tru9I TTAA 2 cut(s) 12, 654
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 3 cut(s) 233, 311, 512
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 27, 541
VpaK11BI GGWCC 1 cut(s) 323
VspI ATTAAT 1 cut(s) 12
XspI CTAG 2 cut(s) 308, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.