Rh2DG337500

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004544
Physical Location & Seq
Forward (+)
43386321 .. 43390706
4386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG337500.1

Sequence Viewer

Length: 552 bp
ATGCAACTCAGAGTCGTGCAGCTCCGGTGTGGCGGCTTGAAGTCGACTCCGGTGCCGAGTTCGGGTCTGCAACGATTGCGACGACCGGAGTTCCATCGAGGTCGAGGAGGAAGGCCAGGGGATTCATGGGGTTGTTATGAGCCTCGTGTGATTCTAGACTTGTTTAAGGAAATGTGCATTGGTGGTTTTGAAGCTAGTGTAACCACTGTTCTGTATCTTTTATCAGCCATTGAGTTAGAGACACCCACCCGTGCTGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTGGTGACATTATTGGCCTTGAATTAAAAGGAGATAATGCACTTATTGCTGCTGATATTGAGCGTTCTTCACTTGTTTTATGTGGCAATGCATTCTCATATTCAAATGCAACTAAAGAAGCACTAGCTGTCTTGTCCAGACCTGTTATATTTGCCCGAAGAGGCCTTCCCCTATCTGCAAGGTACAGTTATTCATTCTCCTTTGGAGCACTAGAGTTTTATTTTGGATGCACTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

183

Amino Acids

19.94

Weight (kDa)

8.65

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 52
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 44
AciI CCGC 1 cut(s) 33
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 62, 314
AgsI TTSAA 4 cut(s) 40, 191, 335, 417
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 3 cut(s) 22, 194, 440
AluI AGCT 3 cut(s) 22, 194, 440
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 2 cut(s) 233, 262
AoxI GGCC 3 cut(s) 113, 328, 475
ApeKI GCWGC 2 cut(s) 19, 362
AsuHPI GGTGA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 52
BauI CACGAG 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 2 cut(s) 31, 349
BccI CCATC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BciT130I CCWGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 233, 262
BfaI CTAG 4 cut(s) 155, 195, 437, 524
BisI GCNGC 3 cut(s) 20, 34, 363
BlsI GCNGC 3 cut(s) 21, 35, 364
Bme1390I CCNGG 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 116
BsaWI WCCGGW 3 cut(s) 24, 49, 85
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 314
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 2 cut(s) 62, 314
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 2 cut(s) 31, 349
BsgI GTGCAG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 115, 330, 477
BshNI GGYRCC 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 3 cut(s) 25, 50, 86
BslI CCNNNNNNNGG 2 cut(s) 62, 314
BsmAI GTCTC 2 cut(s) 233, 262
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 3 cut(s) 115, 330, 477
Bsp1286I GDGCHC 1 cut(s) 523
BspACI CCGC 1 cut(s) 33
BspANI GGCC 3 cut(s) 115, 330, 477
BspCNI CTCAG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 116
BssSI CACGAG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 208, 283, 500
Bst6I CTCTTC 1 cut(s) 466
BstAPI GCANNNNNTGC 2 cut(s) 76, 359
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 1 cut(s) 545
BstMAI GTCTC 2 cut(s) 233, 262
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 2 cut(s) 76, 359
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 31, 349
BsuRI GGCC 3 cut(s) 115, 330, 477
BtsCI GGATG 1 cut(s) 545
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 2 cut(s) 204, 543
CciI TCATGA 1 cut(s) 262
Csp6I GTAC 1 cut(s) 496
CviAII CATG 2 cut(s) 126, 263
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 8
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 406
FaeI CATG 2 cut(s) 129, 266
FaiI YATR 8 cut(s) 127, 138, 264, 288, 302, 394, 412, 461
FatI CATG 2 cut(s) 125, 262
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 3 cut(s) 20, 34, 363
Fsp4HI GCNGC 3 cut(s) 20, 34, 363
FspBI CTAG 4 cut(s) 155, 195, 437, 524
GluI GCNGC 3 cut(s) 20, 34, 363
HaeIII GGCC 3 cut(s) 115, 330, 477
HapII CCGG 3 cut(s) 25, 50, 86
Hin1II CATG 2 cut(s) 129, 266
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 5 cut(s) 12, 46, 122, 151, 266
HpaII CCGG 3 cut(s) 25, 50, 86
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 1 cut(s) 11
Hpy188III TCNNGA 4 cut(s) 155, 263, 308, 450
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 105, 488
HpyCH4III ACNGT 3 cut(s) 208, 283, 500
HpyCH4V TGCA 9 cut(s) 4, 19, 70, 177, 353, 404, 422, 491, 543
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 359
HpyF3I CTNAG 1 cut(s) 8
Hsp92II CATG 2 cut(s) 129, 266
LmnI GCTCC 2 cut(s) 27, 518
LpnPI CCDG 8 cut(s) 38, 63, 99, 102, 129, 321, 463, 468
Lsp1109I GCAGC 2 cut(s) 31, 349
LweI GCATC 1 cut(s) 530
MaeI CTAG 4 cut(s) 155, 195, 437, 524
MaeIII GTNAC 2 cut(s) 199, 317
MboII GAAGA 2 cut(s) 372, 483
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 1 cut(s) 335
MlyI GAGTC 2 cut(s) 21, 40
MnlI CCTC 5 cut(s) 92, 98, 101, 153, 467
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 165, 278, 338
MspI CCGG 3 cut(s) 25, 50, 86
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 76, 359
NlaIII CATG 2 cut(s) 129, 266
NlaIV GGNNCC 1 cut(s) 54
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 406
PagI TCATGA 1 cut(s) 262
PceI AGGCCT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 3 cut(s) 122, 151, 266
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 3 cut(s) 21, 35, 364
PleI GAGTC 2 cut(s) 20, 40
PpsI GAGTC 2 cut(s) 20, 40
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 54
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 3 cut(s) 165, 278, 338
SatI GCNGC 3 cut(s) 20, 34, 363
SchI GAGTC 2 cut(s) 21, 40
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 523
SetI ASST 6 cut(s) 24, 103, 196, 442, 457, 497
SfaNI GCATC 1 cut(s) 530
Sse9I AATT 1 cut(s) 335
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 1 cut(s) 33
SspMI CTAG 4 cut(s) 155, 195, 437, 524
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 3 cut(s) 208, 283, 500
TaqI TCGA 3 cut(s) 44, 97, 103
TasI AATT 1 cut(s) 335
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 3 cut(s) 122, 151, 266
Tru1I TTAA 3 cut(s) 165, 278, 338
Tru9I TTAA 3 cut(s) 165, 278, 338
TscAI CASTG 2 cut(s) 211, 550
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 19, 362
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 114, 495
TspRI CASTG 2 cut(s) 211, 550
Van91I CCANNNNNTGG 1 cut(s) 314
XbaI TCTAGA 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 44
XspI CTAG 4 cut(s) 155, 195, 437, 524
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.