RLG00000026038

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004544
Physical Location & Seq
Reverse (-)
173539 .. 176755
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026038

Sequence Viewer

Length: 1020 bp
ATGAGACTCCTCCGATTACTTCATCGCAGCAGCGTCAACGTGCGGGAATTTTGTGGGGGTCTGAATTGGGGTTTGGCGGGAAGAGGTGTAATTGTAAAGGACAAAGCTTTCCAAAATTTGAACTCCTCTCAACTCTATCTCCATGGCGCAACAGTTGCAGAATCACTGTCTGGACTTGAGGTTTATGTTAGAGGAAATGTCACCGGAGAAGCTCGTAAAATTTCCAAGCCGCAGTTCGGCATACTTTTAAAACAGGTTACCAATCACATATCATCCATGTCAAACATTTATGTCCATGATGGGGCAGTTGGTTCATCACTCAAAGCTGGTGCAAAAGTTCGCGTTATAAGTGATAGCCCATCTGCTGTATTGTCACTTTCCAGCATTCTCTGGGGGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTATTGACCTTGAATTGAAAGGAGATAATGCACTTATAGCTGCTGATATTGAGCCTTCTTCACTTGTTTTATGTGGCAATGCCTTCTCAGATTCAAATGCAACTAAACAAGCACTAGCTGCCTTGTCCGGACCTGTAATATTTGCCCAAAGAGGCCTTCCCCTATCTGCAAGGCTTTTAGTGTTTGGTGATTCTGTGCTTCTTTTATTTGCACCAGAAGATACCATTAAGGGGTGTGTTATTCTTTCTTCTGAAGGTGTTGCCCCATTGTTTCAAACTGGAAGCTCTGGTAGCTCAAATCTATTCAAGTTGCCAGCTGCTGTAGTCCTTGCAACTTTTGACAGTTCTGGTGTTATACCTTCTGTATCAAAGCTTGCACCTGGCCAGGCAGCCTATCACTTCCTGGCTGGCAAAGATTTTGCTCGGTTGGTGGAATCAACTCTGACAAATAACATCCCACCGTTCCAAGCAAAAGGTGGGAATCTTCAAGACACATACAAGAGCTTCTTATCCAGCAGATTTGGAGAAGTGCCGGAAGAATTCTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

340

Amino Acids

35.81

Weight (kDa)

8.71

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 258 - 293 4.2e-07 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 596
AciI CCGC 3 cut(s) 43, 77, 230
AcoI YGGCCR 1 cut(s) 850
AcsI RAATTY 4 cut(s) 47, 115, 219, 1007
AcuI CTGAAG 1 cut(s) 741
AfiI CCNNNNNNNGG 3 cut(s) 236, 301, 699
AgsI TTSAA 7 cut(s) 121, 482, 487, 564, 743, 775, 956
AjnI CCWGG 3 cut(s) 847, 852, 870
AjuI GAANNNNNNNTTGG 6 cut(s) 56, 88, 218, 250, 609, 641
Alw26I GTCTC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 788
Aor13HI TCCGGA 1 cut(s) 596
AoxI GGCC 2 cut(s) 622, 850
ApeKI GCWGC 6 cut(s) 27, 30, 509, 587, 785, 857
ApoI RAATTY 4 cut(s) 47, 115, 219, 1007
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 193, 668
AvaII GGWCC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 852
BbvI GCAGC 6 cut(s) 39, 42, 496, 574, 772, 869
BccI CCATC 2 cut(s) 293, 367
BciT130I CCWGG 3 cut(s) 849, 854, 872
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 789
BisI GCNGC 7 cut(s) 28, 31, 230, 510, 588, 786, 858
BlsI GCNGC 7 cut(s) 29, 32, 231, 511, 589, 787, 859
Bme1390I CCNGG 3 cut(s) 849, 854, 872
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmrFI CCNGG 3 cut(s) 849, 854, 872
BpuEI CTTGAG 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 2 cut(s) 203, 596
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 301, 699
Bse1I ACTGG 1 cut(s) 751
Bse3DI GCAATG 1 cut(s) 553
BseAI TCCGGA 1 cut(s) 596
BseBI CCWGG 3 cut(s) 849, 854, 872
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 272, 921
BseLI CCNNNNNNNGG 3 cut(s) 236, 301, 699
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 751
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 6 cut(s) 39, 42, 496, 574, 772, 869
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 2 cut(s) 624, 852
BsiSI CCGG 3 cut(s) 204, 597, 1001
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 3 cut(s) 236, 301, 699
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 2 cut(s) 624, 852
Bsp13I TCCGGA 1 cut(s) 596
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 3 cut(s) 43, 77, 230
BspANI GGCC 2 cut(s) 624, 852
BspCNI CTCAG 1 cut(s) 569
BspEI TCCGGA 1 cut(s) 596
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 553
BsrI ACTGG 1 cut(s) 751
BssECI CCNNGG 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 3 cut(s) 849, 854, 872
Bst4CI ACNGT 5 cut(s) 154, 168, 439, 812, 930
Bst6I CTCTTC 1 cut(s) 76
BstAPI GCANNNNNTGC 2 cut(s) 155, 587
BstC8I GCNNGC 3 cut(s) 783, 843, 877
BstDEI CTNAG 1 cut(s) 556
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 1 cut(s) 256
BstF5I GGATG 2 cut(s) 272, 921
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 149
BstMAI GTCTC 1 cut(s) 418
BstMWI GCNNNNNNNGC 4 cut(s) 155, 506, 587, 759
BstNI CCWGG 3 cut(s) 849, 854, 872
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 847, 852, 870
BstSFI CTRYAG 1 cut(s) 789
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 6 cut(s) 39, 42, 496, 574, 772, 869
BsuRI GGCC 2 cut(s) 624, 852
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 8
BtsCI GGATG 2 cut(s) 272, 921
BtsIMutI CAGTG 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 783, 843, 877
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 418
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 1 cut(s) 22
CviAII CATG 4 cut(s) 143, 277, 296, 419
DdeI CTNAG 1 cut(s) 556
DraI TTTAAA 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 850
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 741
Eco91I GGTNACC 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 3 cut(s) 847, 852, 870
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 4 cut(s) 146, 280, 299, 422
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FaqI GGGAC 1 cut(s) 409
FatI CATG 4 cut(s) 142, 276, 295, 418
FauI CCCGC 2 cut(s) 36, 70
Fnu4HI GCNGC 7 cut(s) 28, 31, 230, 510, 588, 786, 858
FokI GGATG 2 cut(s) 259, 908
Fsp4HI GCNGC 7 cut(s) 28, 31, 230, 510, 588, 786, 858
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 148
GluI GCNGC 7 cut(s) 28, 31, 230, 510, 588, 786, 858
HaeIII GGCC 2 cut(s) 624, 852
HapII CCGG 3 cut(s) 204, 597, 1001
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 146, 280, 299, 422
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 2 cut(s) 105, 839
HinfI GANTC 7 cut(s) 6, 161, 422, 560, 659, 902, 949
HpaII CCGG 3 cut(s) 204, 597, 1001
HphI GGTGA 2 cut(s) 193, 668
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 6 cut(s) 14, 63, 559, 721, 912, 1019
Hpy188III TCNNGA 5 cut(s) 171, 419, 464, 597, 956
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 5 cut(s) 534, 562, 635, 716, 837
HpyCH4III ACNGT 5 cut(s) 154, 168, 439, 812, 930
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 9 cut(s) 158, 332, 410, 500, 569, 638, 680, 800, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 506, 587, 759
HpyF3I CTNAG 1 cut(s) 556
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 4 cut(s) 146, 280, 299, 422
HspAI GCGC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 596
Lsp1109I GCAGC 6 cut(s) 39, 42, 496, 574, 772, 869
MaeI CTAG 1 cut(s) 584
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 3 cut(s) 199, 256, 372
MboII GAAGA 6 cut(s) 93, 519, 698, 708, 944, 1016
MlsI TGGCCA 1 cut(s) 852
MluCI AATT 7 cut(s) 47, 64, 90, 115, 219, 482, 1007
MluNI TGGCCA 1 cut(s) 852
MnlI CCTC 6 cut(s) 20, 77, 136, 172, 185, 614
Mox20I TGGCCA 1 cut(s) 852
MroI TCCGGA 1 cut(s) 596
MscI TGGCCA 1 cut(s) 852
MseI TTAA 3 cut(s) 248, 434, 696
Msp20I TGGCCA 1 cut(s) 852
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 3 cut(s) 204, 597, 1001
MspR9I CCNGG 3 cut(s) 849, 854, 872
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 3 cut(s) 849, 854, 872
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 155, 506, 587, 759
NcoI CCATGG 1 cut(s) 142
NlaIII CATG 4 cut(s) 146, 280, 299, 422
NmuCI GTSAC 2 cut(s) 199, 372
PagI TCATGA 1 cut(s) 418
PceI AGGCCT 1 cut(s) 624
PctI GAATGC 1 cut(s) 384
PfeI GAWTC 6 cut(s) 161, 422, 560, 659, 902, 949
PkrI GCNGC 7 cut(s) 29, 32, 231, 511, 589, 787, 859
PsiI TTATAA 1 cut(s) 347
Psp6I CCWGG 3 cut(s) 847, 852, 870
PspEI GGTNACC 1 cut(s) 256
PspGI CCWGG 3 cut(s) 847, 852, 870
PspPI GGNCC 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 785
SaqAI TTAA 3 cut(s) 248, 434, 696
SatI GCNGC 7 cut(s) 28, 31, 230, 510, 588, 786, 858
Sau96I GGNCC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 849, 854, 872
SfcI CTRYAG 1 cut(s) 789
SinI GGWCC 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 7 cut(s) 47, 64, 90, 115, 219, 482, 1007
SseBI AGGCCT 1 cut(s) 624
SsiI CCGC 3 cut(s) 43, 77, 230
SspI AATATT 1 cut(s) 609
SspMI CTAG 1 cut(s) 584
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 847, 852, 870
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 5 cut(s) 154, 168, 439, 812, 930
TaiI ACGT 1 cut(s) 42
TasI AATT 7 cut(s) 47, 64, 90, 115, 219, 482, 1007
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 6 cut(s) 161, 422, 560, 659, 902, 949
Tru1I TTAA 3 cut(s) 248, 434, 696
Tru9I TTAA 3 cut(s) 248, 434, 696
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 2 cut(s) 199, 372
TseI GCWGC 6 cut(s) 27, 30, 509, 587, 785, 857
Tsp45I GTSAC 2 cut(s) 199, 372
TspDTI ATGAA 2 cut(s) 11, 303
TspRI CASTG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 4 cut(s) 47, 115, 219, 1007
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.