Rh1CG444500

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004544
Physical Location & Seq
Forward (+)
69633964 .. 69637141
3178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG444500.1

Sequence Viewer

Length: 1206 bp
ATGAGACTCCTCCGATTACTTCATCGCAGCAGCGTGCGGGAATTTTGTGGTGGTCTGAATTGGGGTTTGGCGGGAAGAGGTGTAATTGTAAAGTACAAAGCTTTCCAAAATTTGAACTCCTCGCAACTCTATCTCCATGGCGCAACAGTTGCAGAATCACTGTCTGGACTTGCGGTTTATGTTAGAGGAAATGTCACCGGAGAAGCTCGTAAAATCTCCAAGCCGCAGTTCGGCAAACTTTTAAAACAGGTTACCAATCACATATCATCCATGTCAAACATATATGTCCATGATGGGGCAGTTGGTTCATCACTCAAAGCTGGTGCAAAAGTTCGCGTTATAAGTGATAGCCCATCTGCTGTATTGTCACTTTCCAGCATTCTCTGGGGGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTGGAGACATTATTGGCCTTGAATTGAAAGGAGATAATGCACTTATAGCTGCTGATATTGAGCGTTCTTCACTTGTTTTATGTGGCAATGCGTTCTCAGATTCAAATGCAACTAAACAAGCACTAGCTGCCTTGTCCGGACCTGTTATATTTGCCCGAAGAGGCCTTCCCCTATCTGCAAGGCTTTTAGTGTTTGGTGATTCTGTGCTTCTTTTATTTGCACCAGAAGATACCATTAAGGAGTGTGGAGATCTGTTGGTGCCAGGAGATATAGGTGTTATTCTTTCTTCTGAAGGTGTTGCCCCATTGTTTCAAGCTGGAAGCTCTGGTAGCTCAAATCTATTCAAGTTGCCAGCTGCTGTAGTCCTTGCAACTTTTGACAGTTCTGGTGTTATACCTTCTGTATCAAAGCTTGCACCTGTCCAGGCAGCTTATCACTTCCTGGCTGGTTATCAAAATGGGAAGTTCATGCCTGCATACAGCAAAGGCCCTTCATCTATTGACCTGTTGGAACTAGCAAAGGAGTTTCTGTTGAAGTTGAAAGATGACCGGATCTCATCCTACCTGGTTAATGCCAATGGAGGAGAAAAGGGTGCAACTGGCAAAGATTTTGCTAGGTTGGTGGAATCAACTCTGTCAAATAACATCCCACCGTTCCAAGCAAAAGGTGGGAATCTTCAAGACACATACAAGAGCTTCTTATCCAGCAGATTTGGAGAAGTGCCAGAAGAATTCTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

401

Amino Acids

42.44

Weight (kDa)

9.07

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 269 - 306 7.3e-08 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 721
AccB7I CCANNNNNTGG 1 cut(s) 464
AccII CGCG 1 cut(s) 336
AccIII TCCGGA 1 cut(s) 599
AciI CCGC 4 cut(s) 37, 71, 173, 224
AclWI GGATC 1 cut(s) 1022
AcsI RAATTY 3 cut(s) 41, 109, 1193
AcuI CTGAAG 1 cut(s) 774
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 3 cut(s) 230, 295, 464
AgsI TTSAA 9 cut(s) 115, 485, 490, 567, 776, 808, 997, 1003, 1142
AjnI CCWGG 4 cut(s) 724, 885, 903, 1026
AjuI GAANNNNNNNTTGG 4 cut(s) 50, 82, 212, 244
Alw26I GTCTC 2 cut(s) 412, 462
AlwI GGATC 1 cut(s) 1022
AlwNI CAGNNNCTG 1 cut(s) 821
Aor13HI TCCGGA 1 cut(s) 599
AoxI GGCC 3 cut(s) 478, 625, 949
ApeKI GCWGC 6 cut(s) 27, 30, 512, 590, 818, 890
ApoI RAATTY 3 cut(s) 41, 109, 1193
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 602, 950
AsuHPI GGTGA 2 cut(s) 187, 671
AvaII GGWCC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 721
BbvI GCAGC 6 cut(s) 39, 42, 499, 577, 805, 902
BccI CCATC 2 cut(s) 287, 361
BciT130I CCWGG 4 cut(s) 726, 887, 905, 1028
BcoDI GTCTC 2 cut(s) 412, 462
BfaI CTAG 3 cut(s) 587, 977, 1077
BfmI CTRYAG 1 cut(s) 822
BglII AGATCT 1 cut(s) 712
BisI GCNGC 7 cut(s) 28, 31, 224, 513, 591, 819, 891
BlsI GCNGC 7 cut(s) 29, 32, 225, 514, 592, 820, 892
Bme1390I CCNGG 4 cut(s) 726, 887, 905, 1028
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 602, 950
BmiI GGNNCC 1 cut(s) 723
BmrFI CCNGG 4 cut(s) 726, 887, 905, 1028
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 3 cut(s) 197, 599, 1011
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 977, 1007
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 295, 464
Bse1I ACTGG 1 cut(s) 1066
Bse3DI GCAATG 1 cut(s) 556
BseAI TCCGGA 1 cut(s) 599
BseBI CCWGG 4 cut(s) 726, 887, 905, 1028
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 266, 1019, 1107
BseLI CCNNNNNNNGG 3 cut(s) 230, 295, 464
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 1066
BseRI GAGGAG 2 cut(s) 109, 1059
BseXI GCAGC 6 cut(s) 39, 42, 499, 577, 805, 902
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 3 cut(s) 480, 627, 951
BshNI GGYRCC 1 cut(s) 721
BsiSI CCGG 3 cut(s) 198, 600, 1012
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 3 cut(s) 230, 295, 464
BsmAI GTCTC 2 cut(s) 412, 462
BsmFI GGGAC 1 cut(s) 403
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 3 cut(s) 480, 627, 951
Bsp13I TCCGGA 1 cut(s) 599
Bsp143I GATC 2 cut(s) 712, 1014
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 4 cut(s) 37, 71, 173, 224
BspANI GGCC 3 cut(s) 480, 627, 951
BspCNI CTCAG 1 cut(s) 572
BspEI TCCGGA 1 cut(s) 599
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 723
BspPI GGATC 1 cut(s) 1022
BspT107I GGYRCC 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 556
BsrI ACTGG 1 cut(s) 1066
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 712, 1014
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 4 cut(s) 726, 887, 905, 1028
Bst4CI ACNGT 5 cut(s) 148, 162, 433, 845, 1116
Bst6I CTCTTC 2 cut(s) 70, 616
BstAPI GCANNNNNTGC 2 cut(s) 149, 590
BstC8I GCNNGC 4 cut(s) 35, 816, 876, 936
BstDEI CTNAG 1 cut(s) 559
BstDSI CCRYGG 1 cut(s) 136
BstEII GGTNACC 1 cut(s) 250
BstF5I GGATG 3 cut(s) 266, 1019, 1107
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 715, 1017
BstMAI GTCTC 2 cut(s) 412, 462
BstMBI GATC 2 cut(s) 712, 1014
BstMWI GCNNNNNNNGC 4 cut(s) 149, 509, 590, 792
BstNI CCWGG 4 cut(s) 726, 887, 905, 1028
BstPI GGTNACC 1 cut(s) 250
BstSCI CCNGG 4 cut(s) 724, 885, 903, 1026
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 6 cut(s) 39, 42, 499, 577, 805, 902
BstX2I RGATCY 2 cut(s) 712, 1014
BstYI RGATCY 2 cut(s) 712, 1014
BsuRI GGCC 3 cut(s) 480, 627, 951
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 8
BtsCI GGATG 3 cut(s) 266, 1019, 1107
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 4 cut(s) 35, 816, 876, 936
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 602, 950
CsiI ACCWGGT 1 cut(s) 1026
Csp6I GTAC 1 cut(s) 94
CviAII CATG 5 cut(s) 137, 271, 290, 413, 931
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 559
DpnI GATC 2 cut(s) 714, 1016
DpnII GATC 2 cut(s) 712, 1014
DraI TTTAAA 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 70, 616
EarI CTCTTC 2 cut(s) 70, 616
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 627
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 774
Eco91I GGTNACC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 250
EcoRI GAATTC 1 cut(s) 1193
EcoRII CCWGG 4 cut(s) 724, 885, 903, 1026
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 5 cut(s) 140, 274, 293, 416, 934
FalI AAGNNNNNCTT 2 cut(s) 1145, 1177
FaqI GGGAC 1 cut(s) 403
FatI CATG 5 cut(s) 136, 270, 289, 412, 930
FauI CCCGC 2 cut(s) 30, 64
Fnu4HI GCNGC 7 cut(s) 28, 31, 224, 513, 591, 819, 891
FokI GGATG 3 cut(s) 253, 1006, 1094
Fsp4HI GCNGC 7 cut(s) 28, 31, 224, 513, 591, 819, 891
FspBI CTAG 3 cut(s) 587, 977, 1077
GlaI GCGC 1 cut(s) 142
GluI GCNGC 7 cut(s) 28, 31, 224, 513, 591, 819, 891
HaeIII GGCC 3 cut(s) 480, 627, 951
HapII CCGG 3 cut(s) 198, 600, 1012
HhaI GCGC 1 cut(s) 143
Hin1II CATG 5 cut(s) 140, 274, 293, 416, 934
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 99, 872
HinfI GANTC 7 cut(s) 6, 155, 416, 563, 662, 1088, 1135
HpaII CCGG 3 cut(s) 198, 600, 1012
HphI GGTGA 2 cut(s) 187, 671
Hpy188I TCNGA 5 cut(s) 14, 57, 562, 754, 1205
Hpy188III TCNNGA 5 cut(s) 165, 413, 458, 600, 1142
HpyAV CCTTC 4 cut(s) 638, 749, 870, 963
HpyCH4III ACNGT 5 cut(s) 148, 162, 433, 845, 1116
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 509, 590, 792
HpyF3I CTNAG 1 cut(s) 559
Hsp92II CATG 5 cut(s) 140, 274, 293, 416, 934
HspAI GCGC 1 cut(s) 141
Kpn2I TCCGGA 1 cut(s) 599
Kzo9I GATC 2 cut(s) 712, 1014
Lsp1109I GCAGC 6 cut(s) 39, 42, 499, 577, 805, 902
MabI ACCWGGT 1 cut(s) 1026
MaeI CTAG 3 cut(s) 587, 977, 1077
MaeIII GTNAC 3 cut(s) 193, 250, 366
MalI GATC 2 cut(s) 714, 1016
MboI GATC 2 cut(s) 712, 1014
MboII GAAGA 7 cut(s) 87, 522, 633, 701, 741, 1130, 1202
MflI RGATCY 2 cut(s) 712, 1014
MluCI AATT 6 cut(s) 41, 58, 84, 109, 485, 1193
MmeI TCCRAC 1 cut(s) 951
MnlI CCTC 6 cut(s) 20, 71, 130, 179, 617, 1037
MroI TCCGGA 1 cut(s) 599
MseI TTAA 4 cut(s) 242, 428, 699, 1032
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 3 cut(s) 198, 600, 1012
MspR9I CCNGG 4 cut(s) 726, 887, 905, 1028
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 4 cut(s) 726, 887, 905, 1028
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 149, 509, 590, 792
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 712, 1014
NlaIII CATG 5 cut(s) 140, 274, 293, 416, 934
NlaIV GGNNCC 1 cut(s) 723
NmuCI GTSAC 2 cut(s) 193, 366
PagI TCATGA 1 cut(s) 412
PceI AGGCCT 1 cut(s) 627
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 6 cut(s) 155, 416, 563, 662, 1088, 1135
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 7 cut(s) 29, 32, 225, 514, 592, 820, 892
PsiI TTATAA 1 cut(s) 341
Psp6I CCWGG 4 cut(s) 724, 885, 903, 1026
PspEI GGTNACC 1 cut(s) 250
PspGI CCWGG 4 cut(s) 724, 885, 903, 1026
PspN4I GGNNCC 1 cut(s) 723
PspPI GGNCC 2 cut(s) 602, 950
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 2 cut(s) 712, 1014
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SaqAI TTAA 4 cut(s) 242, 428, 699, 1032
SatI GCNGC 7 cut(s) 28, 31, 224, 513, 591, 819, 891
Sau3AI GATC 2 cut(s) 712, 1014
Sau96I GGNCC 2 cut(s) 602, 950
ScrFI CCNGG 4 cut(s) 726, 887, 905, 1028
SexAI ACCWGGT 1 cut(s) 1026
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 1 cut(s) 602
Sse9I AATT 6 cut(s) 41, 58, 84, 109, 485, 1193
SseBI AGGCCT 1 cut(s) 627
SsiI CCGC 4 cut(s) 37, 71, 173, 224
SspMI CTAG 3 cut(s) 587, 977, 1077
StuI AGGCCT 1 cut(s) 627
StyD4I CCNGG 4 cut(s) 724, 885, 903, 1026
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 5 cut(s) 148, 162, 433, 845, 1116
TasI AATT 6 cut(s) 41, 58, 84, 109, 485, 1193
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 6 cut(s) 155, 416, 563, 662, 1088, 1135
Tru1I TTAA 4 cut(s) 242, 428, 699, 1032
Tru9I TTAA 4 cut(s) 242, 428, 699, 1032
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 2 cut(s) 193, 366
TseI GCWGC 6 cut(s) 27, 30, 512, 590, 818, 890
Tsp45I GTSAC 2 cut(s) 193, 366
TspDTI ATGAA 4 cut(s) 11, 297, 919, 945
TspRI CASTG 1 cut(s) 165
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 3 cut(s) 41, 109, 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 1127
XspI CTAG 3 cut(s) 587, 977, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.