Rorug01G0448100

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004544
Physical Location & Seq
Reverse (-)
54401621 .. 54431017
29397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0448100.1

Sequence Viewer

Length: 4071 bp
ATGAGCACAAATACAGCAGTAGTATCAGTTATGAGGTTCATATTGCTTCTAGTCTTTGTTGGATGGCTCATCGTTTGGATTCTGTTGCCAACCAAAGCTTACGGATATTCCTGGAGACCCAACCTACAAGGCAAACTCAACTCAACATATTTCGGAGATAAAGGGACAAATCTGCTGCTTTTCTCGTTCCCGATGATGTTCATAGCAGCTTTGAGTTGTGTTTATCTCCATCTAATTCAGAAGGCGAAATCATCATACTCGACATACTACTCCACAAGCTTTGCTAGCTTCTGGAGGCGTCCGGCGAGTGTGAAAGCTCCACTGGGAATTGTGACTACGATGGAGCTCGGTTTCGCTGTAATGTTTGTTGCACTCTTGGTGTGGTCTCTCACCAATTACATACATGTCGGGTTTGCCTACCTCAATAATATGTATAAGAAACCAGTTCCGGGCCTGAAAGAGAACGAGTGGGAAGCAAAGCTTCATTATGTATCGTACTGGCTTGGGTTAACCGGAAACATATGTCTGGCATTTCTCTTCTTCCCTGTAACTCGAGGCTCTTCGATTTTGGCTCTGGTTGGCTTAACATCGGAGTCGAGTATCAAGTACCATATTTGGCTCGGCCATCTCTCCATGGTTCTATTTGCTGCTCATTCCCTAGGCTACATCATCTTCTGGGCCATCACTAATCAAATGGCTGAGATGCTAGAATGGAGCAAGACTCATGTATCAAACGTAGCTGGGGAGATTGCAATTCTATTTGCAATGGCAATGTGGGTGACTAGCATTCCTCGTGTTAGACGAAAAATGTTTGAATTATTTTTCTACACCCACCAGCTCTACATTCTCTACATCGTCTTCTACGTACTGCACGCCGGTGAAAACTTCTTCAGTACGATCCTTCCTGGGATCTTCCTCTTCATCATAGATCGGTACCTGAGATTTTTACAGTCTAATCAACGCGCTGAGTTACTCTCCGCACGTATTTTACCTTGTGAGACCATTGAACTGAACTTCTCCAAGAGCCAAGGTCTGGATTATAATCCCACTAGCATCTTGTTTATCAATGTGCCTACTATATCCAAGATGCAATGGCACCCATTTACAGTGACTTCGAACTCTAACATGGAGGCAGACAAGCTAACTGTTGTGATAAAGAGTCTAGGAAGTTGGTCTCAGAAACTTTACCAACAACTTTCTTCTAATTCTATGGATCATATTCAAGTTTCTGTTGAAGGGCCTTATGGACCTAATTCATCTCATTTTCTAAGGCACAAGGCTCTGGTGATGATTAGTGGAGGCAGTGGAATTACTCCGATGATCTCCATAATCCGAGAAATCATTTTCCAAAGCACCAAACCAGACTGCCATGTTCCGCCTGTTCGGTTGATATGTGCCTTCAAGAACTCAGCTGATCTCACAATGCTACAACTCTTGCTTCCAATTTCTGTCACACCTTCTAATTTTACCCAGCTGCAGTTTCAAATTGAAGCATATGTGACCAGGGAGACACAGAAGCTGCCTCCATCAGACTCCCTCAAGCTTCTCCAAACCTTATGGTTTAAGCCAGACCCACTGGACTCACCCATTTCTTTGGTTCTAGGACAAAACACTTGGCTATGGCTTGGTGCTATAATCTCATCCTCATTTGTCTTATTTCTCTTTGTTTTGGGCATTGTCAATCGTTTCTATATATACCCAATCGACCACAACACTGGTGAACACTACCATGTCTCCTTCATTTGCCTGTGGTACATGTTTTTGGTCTGTGCCTCCATTTTCATAGTCTCTAGTGCAGTCTTCATTTGGTGCAAGAGACAGAACGCCATCGAAGAAGGGAAGCGAACTCAGAATGCGAGATCAACACCAACAACTGATATCGAACTTGCGAGTTCTCCCCATAATCATGAGTCCCTGATCGCTCAAGCTACCCAAGTGCATTATGGAACTAGGCCTGACCTGAAGAAAATTCTGTTGGAAACCAAAGGATCTGATATTGGAGTGTTGGTTAGTGGCCCAAGGAAGATGAGGCATGAGGTTGCCACAGTCTGTTCATCCATTTTTGTGGGTTACACTATGATTTGGATCATGATGCCCACAAATACTTACTATCTGCACTGGCTGCCAGACATTAATGCAAAAACAGATTCCACATATTTTGGGCAACAAGGTGCAGGCATACTTATATATACATTTCCTATCCTATTCACTGCCACTTTGGGGTGTTTATTGCTTCATATAAGAAACAGAACTGCGGATAATAACAACCAAAGTGGGGTGAAGAAGTCATCATGGAAACGACCAGTGCTGGTGAAAGGCCCTCTTGGAATTGTTTCATGGATAGAGCTCTCCTTCCTAGCAATGTTCATTGCTCTACTTGTCTGGTCCATCTCTTCTTACTTGCATGGTATGTTTGCAACCATCACCCTACAAGCCGCAGCAAAGCGAGAGCATGTGTGGGAAGCAAAGTTAGAAAGTACAGCACTAATGCTAGGCCTAGTTGGGAACATCTGTCTAGCCTTTCTCTTCTTCCCTGTGGCGCGAGGGTCTTCGATTCTGCAGGTGGTTGGCCTTACATCAGAAGCAAGTATCAAGTATCATATTTGGCTCGGCCATCTTGTCATGATTATCTTCACGGCTCACGGTTTATGTTACATAATATTCTGGGCCAGCACAAGTCAAATTTCACAGATGCTCAAATGGGAGAAGATTGGAATTGCAAATGTTGCTGGAGAAGTGGCATTGCTGGCTGGATTAGCCATGTGGGCTACAAGCTTCCCTCTCATAAGGCGCAAGATCTTCGAGCTTTTTTTCTACACTCATCACCTCTACATTGTCTTCATCGTCTTCTTCGTGTTCCATGTGGGCTTCTCCTACGCTTGCATAATGCTTCCGGGCTTTTACCTCTTCATGATTGATAGGCTGTTGCGCTTCTTGCAATCCCAGCAAAAAATTGGTCTTGTTTCAGCCCGTGTCCTGCCTTGTGAGGTTGTTGAGTTGAGCTTCTCCAAGATCCCAGGACTGAGCTATTCTCCAACAAGCATGATGTTTCTAAATGTACCTGGAATTTCCAAGCTGCAATGGCACCCTTTTACCATTACTTCCAGCAGCACTATGGATCCTGACAACCTAAGCGCCGTCATCAAAAGCGAAGGAAGTTGGTCTCACAATTTATATGAGAAGTTTTCATCAAGCACTTGGCCTATGGACCGCCTCGAGGTCTCAGTTGAAGGACCCTATGGACCTGCCTCTAACAAACTCCTAAGGCACGATACACTAGTGATGGTGAGTGGAGGAAGCGGCCTTACTCCATTGATATCCATAATCCGAGAGCTCCTATCTCAAGCCAATGACTCAGAAAAGAAGACTCCACAAGTTCTGCTCATAACTGCCTTCAAAAAATCACTAGACCTCACTATGTTAGACCTCATCCTCCCGGCTTCAGGCACAAACTTGGACATTTCTTGCTTGCAATTACAGATTGAAGCTTACGTGACGAGAGAGAACCAACCCGTAACACATAACCACAAGGCTCTGCAAACAATATGGTTCAACCCAGACCCATTGGACTCGCCAGTTTCTGCTGTATTAGGCCGAAACAGTTGGATTTGGCTTGGAGTGATCATAGCAACCTCCTTCGTCATTTTCCTCGTGCTAATGGGAATCCTCACACGATATTACATATACCCCATTGACCATAACTCTAATATGATATACTCTGCCTCTTATAGATCTGCCTTAAACATGTTATTGCTATGTGTTTCCATAGCCACAACTGCAACTGCAGTTTTCCTATGGAATAAGAAACAAAATTTAAAGGAGATGGGACAGATTCAAGCCATAGACACACCACCACAGATTACTACTTCACCAAGCGCTCGGTTTTACAATGCTGATCAAGAACTTGAAAGCCTTTCCCACCAGTCCTTTGTCCAATCCACAAAGGTGCACTATGGCACAAGGCCGGATCTTCAAAGTAAGTTTCAAGGCTCAGGGGCAAAGGGGTATGCTGTTCATCGCCCCTTTGCTCAGAAGCATTCTGCAAATCATGCAAAGGATATAATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

1356

Amino Acids

152.88

Weight (kDa)

9.14

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferric_reduct PF01794 168 - 288 2.8e-19 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 323 - 418 7e-24 FAD-binding domain
NAD_binding_6 PF08030 426 - 684 3.7e-18 Ferric reductase NAD binding domain
Ferric_reduct PF01794 831 - 952 2.7e-20 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 987 - 1084 1.1e-22 FAD-binding domain
NAD_binding_6 PF08030 1090 - 1329 2.9e-10 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
AarI CACCTGC 1 cut(s) 2551
AbsI CCTCGAGG 1 cut(s) 3215
Acc36I ACCTGC 2 cut(s) 2551, 3253
Acc65I GGTACC 1 cut(s) 933
AccB1I GGYRCC 3 cut(s) 933, 1095, 3084
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccII CGCG 2 cut(s) 963, 2542
AciI CCGC 6 cut(s) 978, 1376, 2255, 2436, 3211, 3300
AclWI GGATC 9 cut(s) 892, 917, 1221, 1996, 2093, 3007, 3113, 3126, 3975
AcoI YGGCCR 2 cut(s) 622, 2611
AcsI RAATTY 4 cut(s) 1968, 2682, 3066, 3811
AcuI CTGAAG 3 cut(s) 874, 1982, 3426
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 8 cut(s) 497, 608, 867, 895, 935, 1754, 2479, 3060
AfeI AGCGCT 1 cut(s) 3877
AfiI CCNNNNNNNGG 6 cut(s) 449, 1033, 2220, 2275, 3217, 3443
AflIII ACRYGT 3 cut(s) 403, 1755, 3744
AhlI ACTAGT 1 cut(s) 3277
AjnI CCWGG 5 cut(s) 110, 904, 1502, 3016, 3061
AjuI GAANNNNNNNTTGG 2 cut(s) 1958, 1990
AleI CACNNNNGTG 2 cut(s) 876, 3944
Alw21I GWGCWC 5 cut(s) 8, 348, 2349, 3336, 3951
Alw44I GTGCAC 1 cut(s) 3947
AlwI GGATC 9 cut(s) 892, 917, 1221, 1996, 2093, 3007, 3113, 3126, 3975
AlwNI CAGNNNCTG 2 cut(s) 1519, 3581
Ama87I CYCGRG 2 cut(s) 552, 3215
Aor51HI AGCGCT 1 cut(s) 3877
ApaLI GTGCAC 1 cut(s) 3947
ApeKI GCWGC 9 cut(s) 175, 206, 647, 1474, 1519, 2122, 2438, 3076, 3108
ApoI RAATTY 4 cut(s) 1968, 2682, 3066, 3811
ArsI GACNNNNNNTTYG 2 cut(s) 2941, 2973
AseI ATTAAT 1 cut(s) 2133
Asp700I GAANNNNTTC 1 cut(s) 2332
Asp718I GGTACC 1 cut(s) 933
AspA2I CCTAGG 1 cut(s) 658
AspLEI GCGC 6 cut(s) 965, 2542, 2793, 2931, 3137, 3878
AsuC2I CCSGG 3 cut(s) 450, 2895, 3437
AsuII TTCGAA 1 cut(s) 1115
AsuNHI GCTAGC 1 cut(s) 284
AvaI CYCGRG 2 cut(s) 552, 3215
AvaII GGWCC 5 cut(s) 1247, 2385, 3208, 3233, 3242
AvrII CCTAGG 1 cut(s) 658
AxyI CCTNAGG 1 cut(s) 3263
BaeGI GKGCMC 1 cut(s) 3951
BaeI ACNNNNGTAYC 4 cut(s) 711, 744, 3264, 3297
BamHI GGATCC 1 cut(s) 3118
BanI GGYRCC 3 cut(s) 933, 1095, 3084
BanII GRGCYC 3 cut(s) 348, 2349, 3336
BarI GAAGNNNNNNTAC 2 cut(s) 3289, 3321
BauI CACGAG 2 cut(s) 792, 3650
BbsI GAAGAC 6 cut(s) 850, 1792, 2541, 2830, 2839, 3371
Bbv12I GWGCWC 5 cut(s) 8, 348, 2349, 3336, 3951
BbvI GCAGC 9 cut(s) 162, 218, 634, 1461, 1506, 2109, 2450, 3063, 3120
BceAI ACGGC 2 cut(s) 2652, 3122
BcgI CGANNNNNNTGC 2 cut(s) 2782, 2816
BciT130I CCWGG 5 cut(s) 112, 906, 1504, 3018, 3063
BclI TGATCA 2 cut(s) 3621, 3895
BcnI CCSGG 3 cut(s) 450, 2895, 3437
BcuI ACTAGT 1 cut(s) 3277
BfmI CTRYAG 3 cut(s) 1475, 2558, 3783
BfoI RGCGCY 2 cut(s) 3138, 3879
BfuAI ACCTGC 2 cut(s) 2551, 3253
BglI GCCNNNNNGGC 1 cut(s) 2765
BglII AGATCT 2 cut(s) 2796, 3731
BlnI CCTAGG 1 cut(s) 658
Bme1390I CCNGG 8 cut(s) 112, 450, 906, 1504, 2895, 3018, 3063, 3437
Bme18I GGWCC 5 cut(s) 1247, 2385, 3208, 3233, 3242
BmeT110I CYCGRG 2 cut(s) 552, 3215
BmiI GGNNCC 5 cut(s) 935, 1097, 3086, 3120, 3235
BmrFI CCNGG 8 cut(s) 112, 450, 906, 1504, 2895, 3018, 3063, 3437
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 5 cut(s) 693, 1062, 1077, 2082, 2682
BmtI GCTAGC 1 cut(s) 288
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 6 cut(s) 850, 1792, 2541, 2830, 2839, 3371
BplI GAGNNNNNCTC 6 cut(s) 706, 738, 959, 991, 3016, 3048
BpmI CTGGAG 3 cut(s) 133, 313, 2751
Bpu10I CCTNAGC 2 cut(s) 3131, 3991
Bpu14I TTCGAA 1 cut(s) 1115
BpuEI CTTGAG 3 cut(s) 1523, 1908, 3327
BpuMI CCSGG 3 cut(s) 450, 2895, 3437
BsaAI YACGTR 3 cut(s) 865, 983, 3493
BsaBI GATNNNNATC 2 cut(s) 1041, 2084
BsaHI GRCGYC 1 cut(s) 298
BsaI GGTCTC 6 cut(s) 109, 390, 992, 1179, 3168, 3226
BsaJI CCNNGG 7 cut(s) 633, 658, 905, 1027, 1503, 2018, 3016
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 4 cut(s) 1718, 1748, 1992, 2022
Bsc4I CCNNNNNNNGG 6 cut(s) 449, 1033, 2220, 2275, 3217, 3443
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 9 cut(s) 327, 443, 503, 1581, 1720, 2123, 2303, 3575, 3922
Bse21I CCTNAGG 1 cut(s) 3263
Bse3DI GCAATG 7 cut(s) 771, 777, 1097, 2367, 2367, 2741, 3086
Bse8I GATNNNNATC 2 cut(s) 1041, 2084
BseBI CCWGG 5 cut(s) 112, 906, 1504, 3018, 3063
BseDI CCNNGG 7 cut(s) 633, 658, 905, 1027, 1503, 2018, 3016
BseGI GGATG 4 cut(s) 68, 1640, 2054, 3429
BseJI GATNNNNATC 2 cut(s) 1041, 2084
BseLI CCNNNNNNNGG 6 cut(s) 449, 1033, 2220, 2275, 3217, 3443
BseMI GCAATG 7 cut(s) 771, 777, 1097, 2367, 2367, 2741, 3086
BseNI ACTGG 9 cut(s) 327, 443, 503, 1581, 1720, 2123, 2303, 3575, 3922
BseSI GKGCMC 1 cut(s) 3951
BseXI GCAGC 9 cut(s) 162, 218, 634, 1461, 1506, 2109, 2450, 3063, 3120
BseYI CCCAGC 3 cut(s) 740, 1470, 2943
BsgI GTGCAG 4 cut(s) 854, 1815, 2099, 2193
Bsh1236I CGCG 2 cut(s) 963, 2542
BshNI GGYRCC 3 cut(s) 933, 1095, 3084
BsiHKAI GWGCWC 5 cut(s) 8, 348, 2349, 3336, 3951
BsiHKCI CYCGRG 2 cut(s) 552, 3215
BsiSI CCGG 7 cut(s) 302, 449, 513, 876, 2894, 3437, 3965
BslFI GGGAC 3 cut(s) 178, 1897, 3840
BslI CCNNNNNNNGG 6 cut(s) 449, 1033, 2220, 2275, 3217, 3443
BsmFI GGGAC 3 cut(s) 178, 1897, 3840
BsmI GAATGC 3 cut(s) 786, 1858, 4036
Bso31I GGTCTC 6 cut(s) 109, 390, 992, 1179, 3168, 3226
BsoBI CYCGRG 2 cut(s) 552, 3215
Bsp119I TTCGAA 1 cut(s) 1115
Bsp1286I GDGCHC 5 cut(s) 8, 348, 2349, 3336, 3951
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 6 cut(s) 978, 1376, 2255, 2436, 3211, 3300
BspFNI CGCG 2 cut(s) 963, 2542
BspHI TCATGA 4 cut(s) 1906, 2088, 2622, 2910
BspLI GGNNCC 5 cut(s) 935, 1097, 3086, 3120, 3235
BspMAI CTGCAG 3 cut(s) 1479, 2562, 3787
BspMI ACCTGC 2 cut(s) 2551, 3253
BspOI GCTAGC 1 cut(s) 288
BspPI GGATC 9 cut(s) 892, 917, 1221, 1996, 2093, 3007, 3113, 3126, 3975
BspQI GCTCTTC 1 cut(s) 565
BspT104I TTCGAA 1 cut(s) 1115
BspT107I GGYRCC 3 cut(s) 933, 1095, 3084
BspTNI GGTCTC 6 cut(s) 109, 390, 992, 1179, 3168, 3226
BsrDI GCAATG 7 cut(s) 771, 777, 1097, 2367, 2367, 2741, 3086
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 9 cut(s) 327, 443, 503, 1581, 1720, 2123, 2303, 3575, 3922
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 7 cut(s) 633, 658, 905, 1027, 1503, 2018, 3016
BssNI GRCGYC 1 cut(s) 298
BssSI CACGAG 2 cut(s) 792, 3650
BssT1I CCWWGG 4 cut(s) 633, 658, 1027, 2018
Bst2BI CACGAG 2 cut(s) 792, 3650
Bst2UI CCWGG 5 cut(s) 112, 906, 1504, 3018, 3063
Bst4CI ACNGT 6 cut(s) 951, 1108, 1147, 2047, 2645, 3602
Bst6I CTCTTC 6 cut(s) 542, 565, 923, 2398, 2531, 2912
BstACI GRCGYC 1 cut(s) 298
BstAPI GCANNNNNTGC 3 cut(s) 2122, 2726, 4049
BstBAI YACGTR 3 cut(s) 865, 983, 3493
BstBI TTCGAA 1 cut(s) 1115
BstC8I GCNNGC 7 cut(s) 286, 873, 2176, 2671, 2748, 2881, 3470
BstDSI CCRYGG 1 cut(s) 633
BstF5I GGATG 4 cut(s) 68, 1640, 2054, 3429
BstFNI CGCG 2 cut(s) 963, 2542
BstH2I RGCGCY 2 cut(s) 3138, 3879
BstHHI GCGC 6 cut(s) 965, 2542, 2793, 2931, 3137, 3878
BstNI CCWGG 5 cut(s) 112, 906, 1504, 3018, 3063
BstNSI RCATGY 4 cut(s) 407, 1759, 2456, 3748
BstSCI CCNGG 8 cut(s) 110, 448, 904, 1502, 2893, 3016, 3061, 3435
BstSFI CTRYAG 3 cut(s) 1475, 2558, 3783
BstSLI GKGCMC 1 cut(s) 3951
BstSNI TACGTA 1 cut(s) 865
BstUI CGCG 2 cut(s) 963, 2542
BstV1I GCAGC 9 cut(s) 162, 218, 634, 1461, 1506, 2109, 2450, 3063, 3120
BstV2I GAAGAC 6 cut(s) 850, 1792, 2541, 2830, 2839, 3371
BstX2I RGATCY 7 cut(s) 909, 1988, 2796, 3012, 3118, 3731, 3967
BstXI CCANNNNNNTGG 3 cut(s) 1594, 1715, 2065
BstYI RGATCY 7 cut(s) 909, 1988, 2796, 3012, 3118, 3731, 3967
Bsu36I CCTNAGG 1 cut(s) 3263
BtgI CCRYGG 1 cut(s) 633
BtgZI GCGATG 1 cut(s) 4001
BtsCI GGATG 4 cut(s) 68, 1640, 2054, 3429
BtsI GCAGTG 2 cut(s) 1309, 2208
BtsIMutI CAGTG 8 cut(s) 320, 1113, 1309, 1574, 1713, 2116, 2208, 2310
BveI ACCTGC 2 cut(s) 2551, 3253
Cac8I GCNNGC 7 cut(s) 286, 873, 2176, 2671, 2748, 2881, 3470
CaiI CAGNNNCTG 2 cut(s) 1519, 3581
CciI TCATGA 4 cut(s) 1906, 2088, 2622, 2910
CfoI GCGC 6 cut(s) 965, 2542, 2793, 2931, 3137, 3878
Cfr10I RCCGGY 1 cut(s) 875
CseI GACGC 1 cut(s) 287
Csp6I GTAC 8 cut(s) 496, 607, 866, 894, 934, 1753, 2478, 3059
CspCI CAANNNNNGTGG 6 cut(s) 262, 297, 2140, 2175, 2254, 2289
CviQI GTAC 8 cut(s) 496, 607, 866, 894, 934, 1753, 2478, 3059
DraI TTTAAA 1 cut(s) 3816
EaeI YGGCCR 2 cut(s) 622, 2611
Eam1104I CTCTTC 6 cut(s) 542, 565, 923, 2398, 2531, 2912
EarI CTCTTC 6 cut(s) 542, 565, 923, 2398, 2531, 2912
EciI GGCGGA 1 cut(s) 1365
Ecl136II GAGCTC 3 cut(s) 346, 2347, 3334
Eco105I TACGTA 1 cut(s) 865
Eco130I CCWWGG 4 cut(s) 633, 658, 1027, 2018
Eco147I AGGCCT 2 cut(s) 1954, 2496
Eco24I GRGCYC 3 cut(s) 348, 2349, 3336
Eco31I GGTCTC 6 cut(s) 109, 390, 992, 1179, 3168, 3226
Eco32I GATATC 2 cut(s) 1879, 3318
Eco47I GGWCC 5 cut(s) 1247, 2385, 3208, 3233, 3242
Eco47III AGCGCT 1 cut(s) 3877
Eco53kI GAGCTC 3 cut(s) 346, 2347, 3334
Eco57I CTGAAG 3 cut(s) 874, 1982, 3426
Eco81I CCTNAGG 1 cut(s) 3263
Eco88I CYCGRG 2 cut(s) 552, 3215
EcoICRI GAGCTC 3 cut(s) 346, 2347, 3334
EcoO109I RGGNCCY 3 cut(s) 1238, 2318, 3233
EcoRII CCWGG 5 cut(s) 110, 904, 1502, 3016, 3061
EcoRV GATATC 2 cut(s) 1879, 3318
EcoT14I CCWWGG 4 cut(s) 633, 658, 1027, 2018
EcoT38I GRGCYC 3 cut(s) 348, 2349, 3336
ErhI CCWWGG 4 cut(s) 633, 658, 1027, 2018
FalI AAGNNNNNCTT 4 cut(s) 465, 497, 2307, 2339
FaqI GGGAC 3 cut(s) 178, 1897, 3840
FauNDI CATATG 2 cut(s) 521, 1495
FbaI TGATCA 2 cut(s) 3621, 3895
FokI GGATG 4 cut(s) 75, 1627, 2041, 3416
FriOI GRGCYC 3 cut(s) 348, 2349, 3336
GlaI GCGC 6 cut(s) 964, 2541, 2792, 2930, 3136, 3877
GsaI CCCAGC 3 cut(s) 744, 1474, 2947
GsuI CTGGAG 3 cut(s) 133, 313, 2751
HaeII RGCGCY 2 cut(s) 3138, 3879
HapII CCGG 7 cut(s) 302, 449, 513, 876, 2894, 3437, 3965
HgaI GACGC 1 cut(s) 287
HhaI GCGC 6 cut(s) 965, 2542, 2793, 2931, 3137, 3878
Hin1I GRCGYC 1 cut(s) 298
Hin6I GCGC 6 cut(s) 963, 2540, 2791, 2929, 3135, 3876
HinP1I GCGC 6 cut(s) 963, 2540, 2791, 2929, 3135, 3876
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HindIII AAGCTT 6 cut(s) 96, 277, 479, 1541, 2773, 3486
HpaI GTTAAC 1 cut(s) 510
HpaII CCGG 7 cut(s) 302, 449, 513, 876, 2894, 3437, 3965
Hpy166II GTNNAC 3 cut(s) 510, 1721, 3949
Hpy8I GTNNAC 3 cut(s) 510, 1721, 3949
HpyCH4III ACNGT 6 cut(s) 951, 1108, 1147, 2047, 2645, 3602
HpyCH4IV ACGT 4 cut(s) 735, 864, 982, 3492
HpySE526I ACGT 4 cut(s) 735, 864, 982, 3492
Hsp92I GRCGYC 1 cut(s) 298
HspAI GCGC 6 cut(s) 963, 2540, 2791, 2929, 3135, 3876
KpnI GGTACC 1 cut(s) 937
Ksp22I TGATCA 2 cut(s) 3621, 3895
KspAI GTTAAC 1 cut(s) 510
LguI GCTCTTC 1 cut(s) 565
LmnI GCTCC 4 cut(s) 322, 343, 714, 3339
Lsp1109I GCAGC 9 cut(s) 162, 218, 634, 1461, 1506, 2109, 2450, 3063, 3120
LweI GCATC 5 cut(s) 693, 1062, 1077, 2082, 2682
MaeII ACGT 4 cut(s) 735, 864, 982, 3492
MflI RGATCY 7 cut(s) 909, 1988, 2796, 3012, 3118, 3731, 3967
MhlI GDGCHC 5 cut(s) 8, 348, 2349, 3336, 3951
MlyI GAGTC 9 cut(s) 602, 715, 1168, 1526, 1574, 1919, 3347, 3361, 3563
MmeI TCCRAC 4 cut(s) 40, 1956, 3059, 3584
MroXI GAANNNNTTC 1 cut(s) 2332
MseI TTAA 7 cut(s) 509, 584, 1563, 2133, 3740, 3815, 4069
MslI CAYNNNNRTG 5 cut(s) 876, 1728, 1905, 2063, 3944
MspA1I CMGCKG 2 cut(s) 1412, 1474
MspI CCGG 7 cut(s) 302, 449, 513, 876, 2894, 3437, 3965
MspR9I CCNGG 8 cut(s) 112, 450, 906, 1504, 2895, 3018, 3063, 3437
Mva1269I GAATGC 3 cut(s) 786, 1858, 4036
MvaI CCWGG 5 cut(s) 112, 906, 1504, 3018, 3063
MvnI CGCG 2 cut(s) 963, 2542
NciI CCSGG 3 cut(s) 450, 2895, 3437
NcoI CCATGG 1 cut(s) 633
NdeI CATATG 2 cut(s) 521, 1495
NheI GCTAGC 1 cut(s) 284
NlaIV GGNNCC 5 cut(s) 935, 1097, 3086, 3120, 3235
NmeAIII GCCGAG 2 cut(s) 600, 2589
NmuCI GTSAC 6 cut(s) 331, 778, 1108, 1450, 1498, 3493
NspI RCATGY 4 cut(s) 407, 1759, 2456, 3748
NspV TTCGAA 1 cut(s) 1115
OliI CACNNNNGTG 2 cut(s) 876, 3944
PaeR7I CTCGAG 2 cut(s) 552, 3215
PagI TCATGA 4 cut(s) 1906, 2088, 2622, 2910
PaqCI CACCTGC 1 cut(s) 2551
PceI AGGCCT 2 cut(s) 1954, 2496
PciI ACATGT 3 cut(s) 403, 1755, 3744
PciSI GCTCTTC 1 cut(s) 565
PcsI WCGNNNNNNNCGW 3 cut(s) 799, 861, 2850
PctI GAATGC 3 cut(s) 786, 1858, 4036
PdmI GAANNNNTTC 1 cut(s) 2332
PfeI GAWTC 5 cut(s) 79, 2147, 2554, 3663, 3832
PflMI CCANNNNNTGG 1 cut(s) 1033
PfoI TCCNGGA 1 cut(s) 110
PleI GAGTC 9 cut(s) 601, 715, 1167, 1526, 1574, 1918, 3347, 3361, 3563
PpsI GAGTC 9 cut(s) 601, 715, 1167, 1526, 1574, 1918, 3347, 3361, 3563
Ppu21I YACGTR 3 cut(s) 865, 983, 3493
PpuMI RGGWCCY 1 cut(s) 3233
PscI ACATGT 3 cut(s) 403, 1755, 3744
PshBI ATTAAT 1 cut(s) 2133
PsiI TTATAA 1 cut(s) 1041
Psp124BI GAGCTC 3 cut(s) 348, 2349, 3336
Psp5II RGGWCCY 1 cut(s) 3233
Psp6I CCWGG 5 cut(s) 110, 904, 1502, 3016, 3061
PspFI CCCAGC 3 cut(s) 740, 1470, 2943
PspGI CCWGG 5 cut(s) 110, 904, 1502, 3016, 3061
PspN4I GGNNCC 5 cut(s) 935, 1097, 3086, 3120, 3235
PspPPI RGGWCCY 1 cut(s) 3233
PspXI VCTCGAGB 2 cut(s) 552, 3215
PstI CTGCAG 3 cut(s) 1479, 2562, 3787
PstNI CAGNNNCTG 2 cut(s) 1519, 3581
PsuI RGATCY 7 cut(s) 909, 1988, 2796, 3012, 3118, 3731, 3967
PvuII CAGCTG 2 cut(s) 1412, 1474
RsaI GTAC 8 cut(s) 497, 608, 867, 895, 935, 1754, 2479, 3060
RsaNI GTAC 8 cut(s) 496, 607, 866, 894, 934, 1753, 2478, 3059
RseI CAYNNNNRTG 5 cut(s) 876, 1728, 1905, 2063, 3944
SacI GAGCTC 3 cut(s) 348, 2349, 3336
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 7 cut(s) 509, 584, 1563, 2133, 3740, 3815, 4069
SchI GAGTC 9 cut(s) 602, 715, 1168, 1526, 1574, 1919, 3347, 3361, 3563
ScrFI CCNGG 8 cut(s) 112, 450, 906, 1504, 2895, 3018, 3063, 3437
SduI GDGCHC 5 cut(s) 8, 348, 2349, 3336, 3951
SfaNI GCATC 5 cut(s) 693, 1062, 1077, 2082, 2682
SfcI CTRYAG 3 cut(s) 1475, 2558, 3783
Sfr274I CTCGAG 2 cut(s) 552, 3215
SfuI TTCGAA 1 cut(s) 1115
SgrAI CRCCGGYG 1 cut(s) 875
SinI GGWCC 5 cut(s) 1247, 2385, 3208, 3233, 3242
SlaI CTCGAG 2 cut(s) 552, 3215
SmiMI CAYNNNNRTG 5 cut(s) 876, 1728, 1905, 2063, 3944
SmlI CTYRAG 5 cut(s) 552, 1538, 1923, 3215, 3342
SmoI CTYRAG 5 cut(s) 552, 1538, 1923, 3215, 3342
SnaBI TACGTA 1 cut(s) 865
SpeI ACTAGT 1 cut(s) 3277
SseBI AGGCCT 2 cut(s) 1954, 2496
SsiI CCGC 6 cut(s) 978, 1376, 2255, 2436, 3211, 3300
SspI AATATT 1 cut(s) 2661
SstI GAGCTC 3 cut(s) 348, 2349, 3336
StuI AGGCCT 2 cut(s) 1954, 2496
StyD4I CCNGG 8 cut(s) 110, 448, 904, 1502, 2893, 3016, 3061, 3435
StyI CCWWGG 4 cut(s) 633, 658, 1027, 2018
TaaI ACNGT 6 cut(s) 951, 1108, 1147, 2047, 2645, 3602
TaiI ACGT 4 cut(s) 738, 867, 985, 3495
TatI WGTACW 1 cut(s) 2477
TauI GCSGC 2 cut(s) 2438, 3303
TfiI GAWTC 5 cut(s) 79, 2147, 2554, 3663, 3832
Tru1I TTAA 7 cut(s) 509, 584, 1563, 2133, 3740, 3815, 4069
Tru9I TTAA 7 cut(s) 509, 584, 1563, 2133, 3740, 3815, 4069
TscAI CASTG 8 cut(s) 327, 1113, 1309, 1581, 1720, 2123, 2215, 2310
TseFI GTSAC 6 cut(s) 331, 778, 1108, 1450, 1498, 3493
TseI GCWGC 9 cut(s) 175, 206, 647, 1474, 1519, 2122, 2438, 3076, 3108
Tsp45I GTSAC 6 cut(s) 331, 778, 1108, 1450, 1498, 3493
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 8 cut(s) 327, 1113, 1309, 1581, 1720, 2123, 2215, 2310
Van91I CCANNNNNTGG 1 cut(s) 1033
VneI GTGCAC 1 cut(s) 3947
VpaK11BI GGWCC 5 cut(s) 1247, 2385, 3208, 3233, 3242
VspI ATTAAT 1 cut(s) 2133
XapI RAATTY 4 cut(s) 1968, 2682, 3066, 3811
XceI RCATGY 4 cut(s) 407, 1759, 2456, 3748
XcmI CCANNNNNNNNNTGG 3 cut(s) 1940, 2951, 3112
XhoI CTCGAG 2 cut(s) 552, 3215
XmaJI CCTAGG 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 2332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.