RchiOBHm_Chr1g0384481

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004544
Physical Location & Seq
Forward (+)
68303404 .. 68308643
5240 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60731

Sequence Viewer

Length: 885 bp
ATGTCAAACATATATGTCCATGATGGGGCAGTTGGTTCATCACTCAAAGCTGGTGCGAAAGTTCGCGTTATAAGTGATAGCCCATCTGCTGTATTGTCACTTTCCAGCATTCTCTGGGGGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTGGAGACATTATTGGCCTTGAATTGAAAGGAGATAATGCATTTATAGCTGCTGATATTGAGCGTTCTTCACTTGTTTTATGTGGCAATGCCTTCTCAGATTCAAATGCAACTAAACAAGCACTAGCTGCCTTGTCTGGACCTGTTATATTTGCCCGAAGAGGCCTTCCCCTGTCTGCAAGGCTTTTAGTGTGTGGAGATCTGTTGGTGCCAGGAGATATAGGTGTTATTCTTTCTTCTGAAGGTGTTGCCCCATTGTTTCAAACTGGAAGCTCTGGTAGCTCAAATCTATTCAAGTTGCCAGTTGCTGTAGTCCTTGCAACTTTTGACAGTTCTGGTGTTATACCTTCTGTATCAAAGCTTGCACCTGGCCAGGCAGCTTATCACTTCCTGGCTGGTTATCAAAATGGGAAGTTCATGCCTGCATACAGCAAAGGCCCTTCATCTATTGACCTGTTGGAACTAGCAAAGGAGTTTCTGTTGAAGTTGAAAGATGACCGGATCTCATCCTACCTGGTTAATGCCAATGGAGGAGAAAAGGGTGCAACTGGCAAAGATTTTGCTCGGTTGGTGGAATCAACTCTGTCAAATAACATCCCACCGTTCCAAGCAAAAGGTGGGAATCTTCAAGACACATACAAGAGCTTCTTATCCAGCAGATTTGGAGAAGTGCCAGAAGAATTCTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

294

Amino Acids

30.73

Weight (kDa)

6.31

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 163 - 199 6.3e-08 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 194
AccII CGCG 1 cut(s) 66
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 1 cut(s) 453
AfiI CCNNNNNNNGG 2 cut(s) 25, 194
AgsI TTSAA 8 cut(s) 215, 220, 297, 455, 487, 676, 682, 821
AjnI CCWGG 5 cut(s) 403, 559, 564, 582, 705
AluBI AGCT 8 cut(s) 50, 242, 320, 465, 474, 553, 572, 837
AluI AGCT 8 cut(s) 50, 242, 320, 465, 474, 553, 572, 837
Alw26I GTCTC 2 cut(s) 142, 192
AlwI GGATC 1 cut(s) 701
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 4 cut(s) 208, 355, 562, 628
ApeKI GCWGC 3 cut(s) 242, 320, 569
ApoI RAATTY 1 cut(s) 872
AspS9I GGNCC 2 cut(s) 332, 629
AvaII GGWCC 1 cut(s) 332
BalI TGGCCA 1 cut(s) 564
BanI GGYRCC 1 cut(s) 400
BbvI GCAGC 3 cut(s) 229, 307, 581
BccI CCATC 2 cut(s) 17, 91
BciT130I CCWGG 5 cut(s) 405, 561, 566, 584, 707
BcoDI GTCTC 2 cut(s) 142, 192
BfaI CTAG 2 cut(s) 317, 656
BfmI CTRYAG 1 cut(s) 501
BglII AGATCT 1 cut(s) 391
BisI GCNGC 3 cut(s) 243, 321, 570
BlsI GCNGC 3 cut(s) 244, 322, 571
Bme1390I CCNGG 5 cut(s) 405, 561, 566, 584, 707
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 332, 629
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 5 cut(s) 405, 561, 566, 584, 707
BsaWI WCCGGW 1 cut(s) 690
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 194
Bse1I ACTGG 3 cut(s) 463, 494, 745
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 5 cut(s) 405, 561, 566, 584, 707
BseGI GGATG 2 cut(s) 698, 786
BseLI CCNNNNNNNGG 2 cut(s) 25, 194
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 3 cut(s) 463, 494, 745
BseRI GAGGAG 1 cut(s) 738
BseXI GCAGC 3 cut(s) 229, 307, 581
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 210, 357, 564, 630
BshNI GGYRCC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 691
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 25, 194
BsmAI GTCTC 2 cut(s) 142, 192
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 4 cut(s) 210, 357, 564, 630
Bsp143I GATC 2 cut(s) 391, 693
BspANI GGCC 4 cut(s) 210, 357, 564, 630
BspCNI CTCAG 1 cut(s) 302
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 1 cut(s) 701
BspT107I GGYRCC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 3 cut(s) 463, 494, 745
BssMI GATC 2 cut(s) 391, 693
Bst2UI CCWGG 5 cut(s) 405, 561, 566, 584, 707
Bst4CI ACNGT 3 cut(s) 163, 524, 795
Bst6I CTCTTC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 555, 615
BstDEI CTNAG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 698, 786
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 2 cut(s) 394, 696
BstMAI GTCTC 2 cut(s) 142, 192
BstMBI GATC 2 cut(s) 391, 693
BstMWI GCNNNNNNNGC 3 cut(s) 239, 320, 471
BstNI CCWGG 5 cut(s) 405, 561, 566, 584, 707
BstSCI CCNGG 5 cut(s) 403, 559, 564, 582, 705
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 229, 307, 581
BstX2I RGATCY 2 cut(s) 391, 693
BstYI RGATCY 2 cut(s) 391, 693
BsuRI GGCC 4 cut(s) 210, 357, 564, 630
BtsCI GGATG 2 cut(s) 698, 786
Cac8I GCNNGC 2 cut(s) 555, 615
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 332, 629
CsiI ACCWGGT 1 cut(s) 705
CviAII CATG 3 cut(s) 20, 143, 610
DdeI CTNAG 1 cut(s) 289
DpnI GATC 2 cut(s) 393, 695
DpnII GATC 2 cut(s) 391, 693
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 629
EcoRI GAATTC 1 cut(s) 872
EcoRII CCWGG 5 cut(s) 403, 559, 564, 582, 705
EcoT22I ATGCAT 1 cut(s) 235
FaeI CATG 3 cut(s) 23, 146, 613
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 19, 142, 609
Fnu4HI GCNGC 3 cut(s) 243, 321, 570
FokI GGATG 2 cut(s) 685, 773
Fsp4HI GCNGC 3 cut(s) 243, 321, 570
FspBI CTAG 2 cut(s) 317, 656
GluI GCNGC 3 cut(s) 243, 321, 570
HaeIII GGCC 4 cut(s) 210, 357, 564, 630
HapII CCGG 1 cut(s) 691
Hin1II CATG 3 cut(s) 23, 146, 613
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 4 cut(s) 146, 293, 767, 814
HpaII CCGG 1 cut(s) 691
Hpy188I TCNGA 3 cut(s) 292, 433, 884
Hpy188III TCNNGA 4 cut(s) 143, 188, 330, 821
HpyAV CCTTC 5 cut(s) 295, 368, 428, 549, 642
HpyCH4III ACNGT 3 cut(s) 163, 524, 795
HpyCH4V TGCA 8 cut(s) 134, 233, 302, 371, 512, 557, 617, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 320, 471
HpyF3I CTNAG 1 cut(s) 289
Hsp92II CATG 3 cut(s) 23, 146, 613
Kzo9I GATC 2 cut(s) 391, 693
Lsp1109I GCAGC 3 cut(s) 229, 307, 581
MabI ACCWGGT 1 cut(s) 705
MaeI CTAG 2 cut(s) 317, 656
MaeIII GTNAC 1 cut(s) 96
MalI GATC 2 cut(s) 393, 695
MboI GATC 2 cut(s) 391, 693
MboII GAAGA 5 cut(s) 252, 363, 420, 809, 881
MflI RGATCY 2 cut(s) 391, 693
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 2 cut(s) 215, 872
MluNI TGGCCA 1 cut(s) 564
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 2 cut(s) 347, 716
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 235
MscI TGGCCA 1 cut(s) 564
MseI TTAA 2 cut(s) 158, 711
Msp20I TGGCCA 1 cut(s) 564
MspI CCGG 1 cut(s) 691
MspR9I CCNGG 5 cut(s) 405, 561, 566, 584, 707
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 5 cut(s) 405, 561, 566, 584, 707
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 239, 320, 471
NdeII GATC 2 cut(s) 391, 693
NlaIII CATG 3 cut(s) 23, 146, 613
NlaIV GGNNCC 1 cut(s) 402
NmuCI GTSAC 1 cut(s) 96
NsiI ATGCAT 1 cut(s) 235
PagI TCATGA 1 cut(s) 142
PceI AGGCCT 1 cut(s) 357
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 4 cut(s) 146, 293, 767, 814
PflMI CCANNNNNTGG 1 cut(s) 194
PkrI GCNGC 3 cut(s) 244, 322, 571
PsiI TTATAA 1 cut(s) 71
Psp6I CCWGG 5 cut(s) 403, 559, 564, 582, 705
PspGI CCWGG 5 cut(s) 403, 559, 564, 582, 705
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 2 cut(s) 332, 629
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 2 cut(s) 391, 693
SaqAI TTAA 2 cut(s) 158, 711
SatI GCNGC 3 cut(s) 243, 321, 570
Sau3AI GATC 2 cut(s) 391, 693
Sau96I GGNCC 2 cut(s) 332, 629
ScrFI CCNGG 5 cut(s) 405, 561, 566, 584, 707
SexAI ACCWGGT 1 cut(s) 705
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 1 cut(s) 332
Sse9I AATT 2 cut(s) 215, 872
SseBI AGGCCT 1 cut(s) 357
SspMI CTAG 2 cut(s) 317, 656
StuI AGGCCT 1 cut(s) 357
StyD4I CCNGG 5 cut(s) 403, 559, 564, 582, 705
TaaI ACNGT 3 cut(s) 163, 524, 795
TasI AATT 2 cut(s) 215, 872
TfiI GAWTC 4 cut(s) 146, 293, 767, 814
Tru1I TTAA 2 cut(s) 158, 711
Tru9I TTAA 2 cut(s) 158, 711
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 3 cut(s) 242, 320, 569
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 27, 598, 624
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XspI CTAG 2 cut(s) 317, 656
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.