Rorug05G0567200

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004544
Physical Location & Seq
Reverse (-)
76044630 .. 76047449
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0567200.1

Sequence Viewer

Length: 2703 bp
ATGGCCTCCAGCTCCCAAATAGCCTGTGCATCTCCTCCTTCATCAGATCCAAGTTGGGAGAATGATGTGTTTTTGAGTTTCAGGGGTGTAGACACTCGCAAGGGGATTACAGTCGACATACACGACCGACTTACTAGGAGAGGAGTTAATACGTTCCTGGATGAGCAAAACCTTAGAGTAGGCGATGCTATTTCTCCCACTCTTCTAAAGGCAATTAAAGAATCAAGGTTTGCAATTGTTGTTCTCTCGAAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTGAGTGCATGAAAGACCATAACAGAATTTTGCCACTATTTTATCATGTTGATCCTACTGATGTTCGATATCAGAAAAACAGTTTCGGAGACGCTTTCATTAAGCATGAAAACTCTCGGCGACACAAATCAAAGAAGATGAAACAATGGAGAGATGCTTTAAAGAAAGTGGCCGGTTTCTCGGGGTGGAATACACAAGATTATAAGACTCACAAACAACTTGTTGACGTCATTGTGGAATTTATCTGCAGTATAGTAGTACCTGATGCAATTGAGTCCACGGGAGATTTCGAAGCATTTGAAGTAACAAAACAAGCCATGTATGAGATTATGAAGGCGTCTACAGATGATGACGTCACTGCAATTGGTGTCTACGGCATGGGAGGCGTTGGAAAGACAACCATGGTAAAACATGTCGCTGCACATGCTAGAAAAAGTGGGATTTTTGATCATGTGATTATGGGTGTCGTATCCCAAAACCCTGACTTGAAAAGAATTCAAGACACATTGGCAGAGTTGCTGGGCTTCGAATTACATGTGGGGACAGAAATTGGAAGAGCAGCTAGATTGAGCAAGGAGATAAAGAGAAGAAATAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGAATTGTCAGACATAGGAATACCTAGCTACAAGGAACTTCGAAAGTGCAAGTCCAAAGTGCTACTCACCACAAGGATAAGAAATGTGTGTCATGCGATGAAGTGCCAAGAAAAGATCAACCTCACTACCCTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTCGAGGATGTTGCGAGGAGGGTAGCTGGAGAGTGTTGCGGTTTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGAGATAAAGACTTGGTGGAATGGAAGAGAGCAGCTCAAAGATTAGAGAAGTCTCATACTGCCAACCCTGAGGATTACGGAGAGGCATCCAAATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTATAAGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTTTGAAATCGAAATAGAAGACTTGTTCAAGTATGCGATCGGGAAAGGGTTGTTTCGAGATGCCGACACAATCGAAGAAGCCAGAGACGAAGCTGATTCCGTGGCCACACACCTTAAACATTGTTGCTTGCTTTTGGATGGTGAAAACAATAAATTTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAAATTGCACAATCTGAAGATGGGCATGGGTTTTTGGTGAAAGCTGGCTGTGGTTTAAAGGATTGGCCTCGTCGATTACATGAAGGCTATTCGGCAATCTCACTAATGGCTAATTGTATTGCCAAGCTACCTGAAAAGTTGGTATGTCCAAAACTCCAAATTTTGTTACTCAACTCTAATTCCTCCATAGGAGAGATCCCAGAATCCTTTTTTCAAAGTCCGAATGAATTAACGGTCTTAGATCTTACGCGGACCCGTATTTCATTACTACCCCAATCATTCAGTCTGCTAACCAATCTCCAAGCTTTGTATTTAGATCGTTGTTTCAAATTTATTGACACTTCTAAACTGGGAGAACTTAAGAAGCTTGAGATTCTTAGTATGAGAAATTCATGCTTTAAGGGGTTGTCGAGAGAAATAGGAGATTTGACCAATCTAAGGATGTTGGATATCACCAGTAAAAGTATTGACACAGTTCCATCCAAAGTGATATCAAAGTTGCATAATTTAGAAGAACTGTACATGCAGGGATTTTGGAACTGGGGGAGTAAAGTTGTGGGAGAAGGAGACGAAACCAATGCGGGCTTTGACGAGGTAACTAGCTTGTCAAATTTAAGAGTTTTGGAGGTTTGTATCCCCGATGCAGAATGTATCCCTAAAGATGTTGAGTTCAATCCGAATTGGGTTAAGTTTAATATATGTATCGGCAGAGACGACAAGATGAAAGCCACTGATCAATATGCTCATAATTCAATATTCTTGAAGATTGACACTATCAGTAACTTACCAGACTGGTTTTGCAATGCGGTGATGACCATAGCAGGGAGCCTACAGTGGAGAGGGTGCCAAAGGCTTATTGAATGTTTTATGGAATATGAGCATGGAAGGTTACATGGATTGAAGCATCTCTCTGTATACGGTGACTATGACCACGAGCTGATGAGCGTGTTAACATGGGTTCCGAAAGAACCTGTGTTTGAGAACTTGGAAGAGCTGCGTCTGATTGGCGTAGATTGCCGGGAATTATGTGTTGGTGAATTACCACCTGGGTCTTTGTTCAATTTGAAGTTATTAGAGGTAAATTGTTACAACTGGGGGATCAATGTACTTTTACCATCAACATTGTTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

900

Amino Acids

102.08

Weight (kDa)

5.83

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 181 3.2e-44 TIR domain
TIR_2 PF13676 23 - 118 1.4e-16 TIR domain
NB-ARC PF00931 213 - 367 3.2e-30 NB-ARC domain
LRR_8 PF13855 573 - 630 1.5e-09 Leucine rich repeat
LRR_14 PF23598 579 - 698 1.8e-12 Leucine-rich repeat region
LRR_14 PF23598 636 - 733 3.9e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 501, 902
AatII GACGTC 2 cut(s) 528, 654
AccB1I GGYRCC 1 cut(s) 2412
AccI GTMKAC 5 cut(s) 90, 114, 638, 669, 2484
AccII CGCG 1 cut(s) 1819
AciI CCGC 4 cut(s) 1164, 1819, 2150, 2375
AclWI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
AcoI YGGCCR 2 cut(s) 468, 1479
AcsI RAATTY 8 cut(s) 324, 536, 792, 1529, 1728, 1898, 1957, 2179
AcuI CTGAAG 1 cut(s) 1605
AcyI GRCGYC 3 cut(s) 525, 635, 651
AfaI GTAC 3 cut(s) 558, 2090, 2676
AfiI CCNNNNNNNGG 2 cut(s) 2007, 2391
AflII CTTAAG 1 cut(s) 1928
AflIII ACRYGT 2 cut(s) 709, 832
AhdI GACNNNNNGTC 1 cut(s) 1339
AjnI CCWGG 3 cut(s) 156, 906, 2614
AjuI GAANNNNNNNTTGG 2 cut(s) 2583, 2615
Alw26I GTCTC 5 cut(s) 381, 1260, 1455, 2130, 2274
AlwI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 4 cut(s) 3, 468, 1479, 1634
ApeKI GCWGC 4 cut(s) 716, 857, 1235, 2563
ApoI RAATTY 8 cut(s) 324, 536, 792, 1529, 1728, 1898, 1957, 2179
AspS9I GGNCC 2 cut(s) 1080, 1821
AsuC2I CCSGG 2 cut(s) 1556, 2588
AsuHPI GGTGA 7 cut(s) 988, 1529, 1618, 2014, 2389, 2501, 2615
AsuII TTCGAA 3 cut(s) 588, 825, 970
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 2 cut(s) 1080, 1821
AxyI CCTNAGG 1 cut(s) 1272
BaeGI GKGCMC 1 cut(s) 1201
BalI TGGCCA 1 cut(s) 1481
BanI GGYRCC 1 cut(s) 2412
BauI CACGAG 1 cut(s) 2501
BbsI GAAGAC 2 cut(s) 1380, 1401
BbvI GCAGC 4 cut(s) 703, 869, 1247, 2550
BccI CCATC 4 cut(s) 1508, 1583, 2056, 2692
BceAI ACGGC 1 cut(s) 688
BcgI CGANNNNNNTGC 4 cut(s) 1118, 1152, 2129, 2163
BciT130I CCWGG 3 cut(s) 158, 908, 2616
BciVI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BclI TGATCA 2 cut(s) 745, 2302
BcnI CCSGG 2 cut(s) 1556, 2588
BcoDI GTCTC 5 cut(s) 381, 1260, 1455, 2130, 2274
BfaI CTAG 5 cut(s) 135, 726, 861, 954, 2169
BfmI CTRYAG 3 cut(s) 544, 639, 2399
BfrI CTTAAG 1 cut(s) 1928
BfuI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BglII AGATCT 1 cut(s) 1810
BisI GCNGC 4 cut(s) 717, 858, 1236, 2564
BlsI GCNGC 4 cut(s) 718, 859, 1237, 2565
Bme1390I CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
Bme18I GGWCC 2 cut(s) 1080, 1821
BmeRI GACNNNNNGTC 1 cut(s) 1339
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 1080, 1821
BmiI GGNNCC 4 cut(s) 1823, 2396, 2414, 2529
BmrFI CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
BmrI ACTGGG 3 cut(s) 1928, 2119, 2671
BmsI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2200, 2482
BmuI ACTGGG 3 cut(s) 1928, 2119, 2671
BpiI GAAGAC 2 cut(s) 1380, 1401
BplI GAGNNNNNCTC 2 cut(s) 1222, 1254
BpmI CTGGAG 1 cut(s) 1173
Bpu14I TTCGAA 3 cut(s) 588, 825, 970
BpuEI CTTGAG 1 cut(s) 1958
BpuMI CCSGG 2 cut(s) 1556, 2588
BsaHI GRCGYC 3 cut(s) 525, 635, 651
BsaJI CCNNGG 4 cut(s) 576, 699, 1476, 2615
Bsc4I CCNNNNNNNGG 2 cut(s) 2007, 2391
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
Bse21I CCTNAGG 1 cut(s) 1272
Bse3DI GCAATG 1 cut(s) 2377
BseBI CCWGG 3 cut(s) 158, 908, 2616
BseDI CCNNGG 4 cut(s) 576, 699, 1476, 2615
BseGI GGATG 7 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048
BseLI CCNNNNNNNGG 2 cut(s) 2007, 2391
BseMI GCAATG 1 cut(s) 2377
BseMII CTCAG 1 cut(s) 1263
BseNI ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
BseRI GAGGAG 3 cut(s) 24, 156, 1156
BseSI GKGCMC 1 cut(s) 1201
BseXI GCAGC 4 cut(s) 703, 869, 1247, 2550
BseYI CCCAGC 1 cut(s) 817
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 1819
Bsh1285I CGRYCG 2 cut(s) 127, 1416
BshFI GGCC 4 cut(s) 5, 470, 1481, 1636
BshNI GGYRCC 1 cut(s) 2412
BsiEI CGRYCG 2 cut(s) 127, 1416
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 3 cut(s) 471, 1555, 2587
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 2 cut(s) 2007, 2391
BsmAI GTCTC 5 cut(s) 381, 1260, 1455, 2130, 2274
BsmBI CGTCTC 4 cut(s) 381, 1455, 2130, 2274
BsmFI GGGAC 1 cut(s) 853
BsnI GGCC 4 cut(s) 5, 470, 1481, 1636
BsoBI CYCGRG 1 cut(s) 478
Bsp119I TTCGAA 3 cut(s) 588, 825, 970
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp1407I TGTACA 1 cut(s) 2088
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 4 cut(s) 1164, 1819, 2150, 2375
BspANI GGCC 4 cut(s) 5, 470, 1481, 1636
BspCNI CTCAG 1 cut(s) 1264
BspFNI CGCG 1 cut(s) 1819
BspLI GGNNCC 4 cut(s) 1823, 2396, 2414, 2529
BspMAI CTGCAG 1 cut(s) 548
BspPI GGATC 5 cut(s) 41, 344, 889, 1759, 2675
BspQI GCTCTTC 2 cut(s) 847, 2553
BspT104I TTCGAA 3 cut(s) 588, 825, 970
BspT107I GGYRCC 1 cut(s) 2412
BspTI CTTAAG 1 cut(s) 1928
BsrDI GCAATG 1 cut(s) 2377
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 2088
BsrI ACTGG 5 cut(s) 1923, 2025, 2114, 2366, 2666
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 576, 699, 1476, 2615
BssNAI GTATAC 1 cut(s) 2485
BssNI GRCGYC 3 cut(s) 525, 635, 651
BssSI CACGAG 1 cut(s) 2501
BssT1I CCWWGG 1 cut(s) 699
Bst1107I GTATAC 1 cut(s) 2485
Bst2BI CACGAG 1 cut(s) 2501
Bst2UI CCWGG 3 cut(s) 158, 908, 2616
Bst4CI ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2489, 2700
Bst6I CTCTTC 4 cut(s) 207, 847, 1223, 2553
BstACI GRCGYC 3 cut(s) 525, 635, 651
BstAFI CTTAAG 1 cut(s) 1928
BstAUI TGTACA 1 cut(s) 2088
BstBI TTCGAA 3 cut(s) 588, 825, 970
BstC8I GCNNGC 3 cut(s) 1505, 1615, 2152
BstDEI CTNAG 6 cut(s) 173, 287, 1272, 1807, 1946, 2006
BstDSI CCRYGG 3 cut(s) 576, 699, 1476
BstF5I GGATG 7 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048
BstFNI CGCG 1 cut(s) 1819
BstMAI GTCTC 5 cut(s) 381, 1260, 1455, 2130, 2274
BstMCI CGRYCG 2 cut(s) 127, 1416
BstMWI GCNNNNNNNGC 4 cut(s) 681, 722, 1184, 2583
BstNI CCWGG 3 cut(s) 158, 908, 2616
BstNSI RCATGY 4 cut(s) 713, 725, 836, 2095
BstSCI CCNGG 5 cut(s) 156, 906, 1554, 2586, 2614
BstSFI CTRYAG 3 cut(s) 544, 639, 2399
BstSLI GKGCMC 1 cut(s) 1201
BstUI CGCG 1 cut(s) 1819
BstV1I GCAGC 4 cut(s) 703, 869, 1247, 2550
BstV2I GAAGAC 2 cut(s) 1380, 1401
BstX2I RGATCY 4 cut(s) 46, 894, 1764, 1810
BstYI RGATCY 4 cut(s) 46, 894, 1764, 1810
BstZ17I GTATAC 1 cut(s) 2485
Bsu36I CCTNAGG 1 cut(s) 1272
BsuI GTATCC 4 cut(s) 778, 1552, 2213, 2231
BsuRI GGCC 4 cut(s) 5, 470, 1481, 1636
BtgI CCRYGG 3 cut(s) 576, 699, 1476
BtgZI GCGATG 2 cut(s) 198, 1040
BtsCI GGATG 7 cut(s) 166, 1138, 1289, 1519, 1545, 2016, 2048
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 654, 2298, 2408
Cac8I GCNNGC 3 cut(s) 1505, 1615, 2152
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 1080, 1821
CseI GACGC 3 cut(s) 398, 624, 2555
Csp6I GTAC 3 cut(s) 557, 2089, 2675
CviQI GTAC 3 cut(s) 557, 2089, 2675
DdeI CTNAG 6 cut(s) 173, 287, 1272, 1807, 1946, 2006
DraI TTTAAA 2 cut(s) 459, 1626
DriI GACNNNNNGTC 1 cut(s) 1339
EaeI YGGCCR 2 cut(s) 468, 1479
Eam1104I CTCTTC 4 cut(s) 207, 847, 1223, 2553
Eam1105I GACNNNNNGTC 1 cut(s) 1339
EarI CTCTTC 4 cut(s) 207, 847, 1223, 2553
Eco130I CCWWGG 1 cut(s) 699
Eco32I GATATC 3 cut(s) 368, 2020, 2061
Eco47I GGWCC 2 cut(s) 1080, 1821
Eco57I CTGAAG 1 cut(s) 1605
Eco81I CCTNAGG 1 cut(s) 1272
Eco88I CYCGRG 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 156, 906, 2614
EcoRV GATATC 3 cut(s) 368, 2020, 2061
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 1 cut(s) 1545
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 4 cut(s) 381, 1455, 2130, 2274
FalI AAGNNNNNCTT 2 cut(s) 1331, 1363
FaqI GGGAC 1 cut(s) 853
FauI CCCGC 1 cut(s) 2143
FbaI TGATCA 2 cut(s) 745, 2302
FblI GTMKAC 5 cut(s) 90, 114, 638, 669, 2484
Fnu4HI GCNGC 4 cut(s) 717, 858, 1236, 2564
FokI GGATG 7 cut(s) 173, 1145, 1276, 1526, 1552, 2023, 2035
Fsp4HI GCNGC 4 cut(s) 717, 858, 1236, 2564
FspBI CTAG 5 cut(s) 135, 726, 861, 954, 2169
GluI GCNGC 4 cut(s) 717, 858, 1236, 2564
GsaI CCCAGC 1 cut(s) 821
GsuI CTGGAG 1 cut(s) 1173
HaeIII GGCC 4 cut(s) 5, 470, 1481, 1636
HapII CCGG 3 cut(s) 471, 1555, 2587
HgaI GACGC 3 cut(s) 398, 624, 2555
Hin1I GRCGYC 3 cut(s) 525, 635, 651
HincII GTYRAC 3 cut(s) 115, 523, 2520
HindII GTYRAC 3 cut(s) 115, 523, 2520
HindIII AAGCTT 2 cut(s) 1872, 1934
HinfI GANTC 8 cut(s) 221, 505, 572, 1073, 1115, 1472, 1772, 1942
HpaI GTTAAC 1 cut(s) 2520
HpaII CCGG 3 cut(s) 471, 1555, 2587
HphI GGTGA 7 cut(s) 988, 1529, 1618, 2014, 2389, 2501, 2615
Hpy166II GTNNAC 9 cut(s) 91, 115, 523, 576, 639, 670, 1169, 2485, 2520
Hpy188III TCNNGA 6 cut(s) 247, 797, 1417, 1433, 1980, 2329
Hpy8I GTNNAC 9 cut(s) 91, 115, 523, 576, 639, 670, 1169, 2485, 2520
Hpy99I CGWCG 1 cut(s) 1644
HpyAV CCTTC 6 cut(s) 48, 625, 1098, 1646, 2126, 2448
HpyCH4III ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2489, 2700
HpyCH4IV ACGT 3 cut(s) 152, 525, 651
HpyF10VI GCNNNNNNNGC 4 cut(s) 681, 722, 1184, 2583
HpyF3I CTNAG 6 cut(s) 173, 287, 1272, 1807, 1946, 2006
HpySE526I ACGT 3 cut(s) 152, 525, 651
Hsp92I GRCGYC 3 cut(s) 525, 635, 651
Ksp22I TGATCA 2 cut(s) 745, 2302
KspAI GTTAAC 1 cut(s) 2520
LguI GCTCTTC 2 cut(s) 847, 2553
LmnI GCTCC 2 cut(s) 17, 2394
Lsp1109I GCAGC 4 cut(s) 703, 869, 1247, 2550
LweI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2200, 2482
MaeI CTAG 5 cut(s) 135, 726, 861, 954, 2169
MaeII ACGT 3 cut(s) 152, 525, 651
MaeIII GTNAC 9 cut(s) 601, 652, 1734, 2164, 2348, 2457, 2489, 2654, 2694
MfeI CAATTG 3 cut(s) 234, 567, 660
MflI RGATCY 4 cut(s) 46, 894, 1764, 1810
MhlI GDGCHC 1 cut(s) 1201
MlsI TGGCCA 1 cut(s) 1481
MluNI TGGCCA 1 cut(s) 1481
MlyI GAGTC 2 cut(s) 499, 581
MmeI TCCRAC 2 cut(s) 667, 1995
Mox20I TGGCCA 1 cut(s) 1481
Mph1103I ATGCAT 1 cut(s) 1545
MscI TGGCCA 1 cut(s) 1481
MslI CAYNNNNRTG 1 cut(s) 1294
Msp20I TGGCCA 1 cut(s) 1481
MspCI CTTAAG 1 cut(s) 1928
MspI CCGG 3 cut(s) 471, 1555, 2587
MspR9I CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
MunI CAATTG 3 cut(s) 234, 567, 660
MvaI CCWGG 3 cut(s) 158, 908, 2616
MvnI CGCG 1 cut(s) 1819
MwoI GCNNNNNNNGC 4 cut(s) 681, 722, 1184, 2583
NciI CCSGG 2 cut(s) 1556, 2588
NcoI CCATGG 1 cut(s) 699
NlaIV GGNNCC 4 cut(s) 1823, 2396, 2414, 2529
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 2 cut(s) 652, 2489
NsiI ATGCAT 1 cut(s) 1545
NspI RCATGY 4 cut(s) 713, 725, 836, 2095
NspV TTCGAA 3 cut(s) 588, 825, 970
PciI ACATGT 2 cut(s) 709, 832
PciSI GCTCTTC 2 cut(s) 847, 2553
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 2280
PfeI GAWTC 6 cut(s) 221, 1073, 1115, 1472, 1772, 1942
PfoI TCCNGGA 3 cut(s) 156, 906, 1554
PkrI GCNGC 4 cut(s) 718, 859, 1237, 2565
Ple19I CGATCG 1 cut(s) 1416
PleI GAGTC 2 cut(s) 499, 580
PpsI GAGTC 2 cut(s) 499, 580
PscI ACATGT 2 cut(s) 709, 832
PsiI TTATAA 2 cut(s) 501, 902
Psp6I CCWGG 3 cut(s) 156, 906, 2614
PspFI CCCAGC 1 cut(s) 817
PspGI CCWGG 3 cut(s) 156, 906, 2614
PspN4I GGNNCC 4 cut(s) 1823, 2396, 2414, 2529
PspPI GGNCC 2 cut(s) 1080, 1821
PstI CTGCAG 1 cut(s) 548
PsuI RGATCY 4 cut(s) 46, 894, 1764, 1810
PvuI CGATCG 1 cut(s) 1416
RsaI GTAC 3 cut(s) 558, 2090, 2676
RsaNI GTAC 3 cut(s) 557, 2089, 2675
RseI CAYNNNNRTG 1 cut(s) 1294
SalI GTCGAC 1 cut(s) 113
SapI GCTCTTC 2 cut(s) 847, 2553
SatI GCNGC 4 cut(s) 717, 858, 1236, 2564
Sau96I GGNCC 2 cut(s) 1080, 1821
SchI GAGTC 2 cut(s) 499, 581
ScrFI CCNGG 5 cut(s) 158, 908, 1556, 2588, 2616
SduI GDGCHC 1 cut(s) 1201
SfaNI GCATC 9 cut(s) 38, 175, 442, 553, 1298, 1426, 1530, 2200, 2482
SfcI CTRYAG 3 cut(s) 544, 639, 2399
SfuI TTCGAA 3 cut(s) 588, 825, 970
SinI GGWCC 2 cut(s) 1080, 1821
SmiMI CAYNNNNRTG 1 cut(s) 1294
SmlI CTYRAG 2 cut(s) 1928, 1937
SmoI CTYRAG 2 cut(s) 1928, 1937
SsiI CCGC 4 cut(s) 1164, 1819, 2150, 2375
SspI AATATT 1 cut(s) 2325
SspMI CTAG 5 cut(s) 135, 726, 861, 954, 2169
StyD4I CCNGG 5 cut(s) 156, 906, 1554, 2586, 2614
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 8 cut(s) 112, 380, 1804, 2044, 2088, 2403, 2489, 2700
TaiI ACGT 3 cut(s) 155, 528, 654
TaqII GACCGA 1 cut(s) 141
TatI WGTACW 2 cut(s) 2088, 2674
TfiI GAWTC 6 cut(s) 221, 1073, 1115, 1472, 1772, 1942
TscAI CASTG 3 cut(s) 661, 2305, 2408
TseFI GTSAC 2 cut(s) 652, 2489
TseI GCWGC 4 cut(s) 716, 857, 1235, 2563
Tsp45I GTSAC 2 cut(s) 652, 2489
TspGWI ACGGA 2 cut(s) 1296, 1465
TspRI CASTG 3 cut(s) 661, 2305, 2408
Vha464I CTTAAG 1 cut(s) 1928
VpaK11BI GGWCC 2 cut(s) 1080, 1821
XapI RAATTY 8 cut(s) 324, 536, 792, 1529, 1728, 1898, 1957, 2179
XceI RCATGY 4 cut(s) 713, 725, 836, 2095
XmiI GTMKAC 5 cut(s) 90, 114, 638, 669, 2484
XspI CTAG 5 cut(s) 135, 726, 861, 954, 2169
ZraI GACGTC 2 cut(s) 526, 652
Zsp2I ATGCAT 1 cut(s) 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.