MD08G1076200.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003649
Physical Location & Seq
Forward (+)
6211377 .. 6212972
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1076200.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGGGAGAGCTCCTTGCTGTGACAAAGCCAACGTGAAGAGAGGTCCATGGTCACCTGAAGAAGATGCCAAGCTCAAGTCTTACATCGAGCAACATGGCACCGGCGGTAACTGGATCGCTTTGCCCCAAAAGATCGGGCTTAAGAGGTGTGGGAAGAGCTGCCGGCTTAGATGGTTAAATTATCTCCGCCCAAACATCAGGCATGGAGGGTTTTCTGAAGAAGAAGACAACATAATTTGCAGCCTCTACATAAGTATTGGAAGCAGGTGGTCTATAATTGCAGCACAACTGCCAGGAAGAACTGACAATGATATAAAGAACTACTGGAACACAAGGCTTAAGAAAAAGCTTCTTGGTAGGCAGCGCAAAGAACATCAGCTGGCTCGTAAATCAGGCCTAGTTAAACAAGACATCATCAAAAGATCAGGCGGCGTTGGTGGAAACAGTACTAACTCCCATTCTGCCGTTCCTGCCACAGACGATCAAAACCCTTACTGGCCGGAGCTTCCAGTGCTTGCAGCACCCACAGTACTACCACAACAACAGCAGCAGCCTTCTTTTAATGACCATGCTTCTCTCAGAAAGATGCTCATCAAGCTTGGAGGGAGGTTTAGTTCAGATATTAATCATAATCATGAGACTAATCAAGTTATCCAAGGTAGGCCCACCAATATTCAACCAACCTATCAAGTTCTTGATGATGATAATAACAGTTCATACGCCGCTGCTGCTGCTACTACTCAGCAAATGGATGAGCACTATTCTGGTAAGAACATGCAAAGTCTCTTAATTCCTCGATGTGGGATTCATGCTCTCAACAATTCTTCTCCTTCCTTAAGCAACAGTGATGTTATCAGCCCTCATCAATTTGCACAAACACATTACATAGTGAATACAGATAACATTGATGGACCTGGTAATGGTATAATGAACAACATGTTTCAAGGGCAAGGTGGTGATTATGAGACCGATCAGCTGAAGGAGATGGCGGTGTATAACAACAATGACAACCAACAGAGATTTGATGGGTTGGAGTTCTTCTGCGGCGAAGATATGATGGTTATGAATAATAGCATTACTAGTAGTTATGGAGAAAGTATTGGGTGGGGTGAGATACTGGGATATCCTCCTGTGGCCTCCTCCTCAGACGACTATCATGACAGCATGGACCGACTGATGCCGGCCACACCCAGCCAAGAATGTGGTTTCGCCAACGACCAGTTGATGAGTGGTTACCCTGGGGAGGCATTATAA

Protein Analysis

418

Amino Acids

46.34

Weight (kDa)

6.06

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 62 2.7e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 7.9e-12 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 112 1.1e-12 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 116 3.3e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1252
AarI CACCTGC 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 98
AciI CCGC 6 cut(s) 105, 187, 429, 723, 989, 1044
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 2 cut(s) 497, 1182
AcuI CTGAAG 3 cut(s) 78, 237, 998
AfaI GTAC 2 cut(s) 448, 531
AfiI CCNNNNNNNGG 3 cut(s) 800, 920, 1243
AflII CTTAAG 3 cut(s) 140, 338, 835
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 2 cut(s) 677, 944
AhlI ACTAGT 1 cut(s) 1079
AjnI CCWGG 3 cut(s) 292, 913, 1237
AloI GAACNNNNNNTCC 2 cut(s) 598, 630
AluBI AGCT 8 cut(s) 11, 73, 159, 349, 379, 505, 598, 976
AluI AGCT 8 cut(s) 11, 73, 159, 349, 379, 505, 598, 976
Alw21I GWGCWC 2 cut(s) 13, 759
Alw26I GTCTC 3 cut(s) 632, 788, 959
AlwI GGATC 1 cut(s) 122
AoxI GGCC 5 cut(s) 394, 497, 662, 1134, 1182
AseI ATTAAT 1 cut(s) 624
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 4 cut(s) 44, 663, 911, 1168
AsuHPI GGTGA 3 cut(s) 45, 968, 1121
AvaII GGWCC 3 cut(s) 44, 911, 1168
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 13, 759
BccI CCATC 5 cut(s) 165, 902, 979, 1019, 1051
BceAI ACGGC 1 cut(s) 449
BciT130I CCWGG 3 cut(s) 294, 915, 1239
BcoDI GTCTC 3 cut(s) 632, 788, 959
BcuI ACTAGT 1 cut(s) 1079
BfaI CTAG 2 cut(s) 398, 1080
BfrI CTTAAG 3 cut(s) 140, 338, 835
BfuAI ACCTGC 1 cut(s) 255
BmcAI AGTACT 2 cut(s) 448, 531
Bme1390I CCNGG 3 cut(s) 294, 915, 1239
Bme18I GGWCC 3 cut(s) 44, 911, 1168
BmgT120I GGNCC 4 cut(s) 44, 663, 911, 1168
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 294, 915, 1239
BmrI ACTGGG 1 cut(s) 1127
BmsI GCATC 3 cut(s) 55, 576, 1167
BmuI ACTGGG 1 cut(s) 1127
BpiI GAAGAC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 59
BsaBI GATNNNNATC 2 cut(s) 590, 624
BsaI GGTCTC 1 cut(s) 959
BsaJI CCNNGG 4 cut(s) 47, 655, 1237, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 3 cut(s) 800, 920, 1243
Bse118I RCCGGY 3 cut(s) 101, 162, 1180
Bse1I ACTGG 6 cut(s) 116, 329, 500, 509, 1122, 1219
Bse8I GATNNNNATC 2 cut(s) 590, 624
BseBI CCWGG 3 cut(s) 294, 915, 1239
BseDI CCNNGG 4 cut(s) 47, 655, 1237, 1238
BseGI GGATG 1 cut(s) 757
BseJI GATNNNNATC 2 cut(s) 590, 624
BseLI CCNNNNNNNGG 3 cut(s) 800, 920, 1243
BseMII CTCAG 3 cut(s) 592, 755, 1158
BseNI ACTGG 6 cut(s) 116, 329, 500, 509, 1122, 1219
BseRI GAGGAG 2 cut(s) 1129, 1132
BseYI CCCAGC 1 cut(s) 1190
BshFI GGCC 5 cut(s) 396, 499, 664, 1136, 1184
BshNI GGYRCC 1 cut(s) 98
BsiHKAI GWGCWC 2 cut(s) 13, 759
BsiSI CCGG 4 cut(s) 102, 163, 500, 1181
BslI CCNNNNNNNGG 3 cut(s) 800, 920, 1243
BsmAI GTCTC 3 cut(s) 632, 788, 959
BsnI GGCC 5 cut(s) 396, 499, 664, 1136, 1184
Bso31I GGTCTC 1 cut(s) 959
Bsp1286I GDGCHC 2 cut(s) 13, 759
Bsp143I GATC 5 cut(s) 114, 132, 422, 481, 970
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 6 cut(s) 105, 187, 429, 723, 989, 1044
BspANI GGCC 5 cut(s) 396, 499, 664, 1136, 1184
BspCNI CTCAG 3 cut(s) 591, 754, 1157
BspHI TCATGA 2 cut(s) 634, 1156
BspLI GGNNCC 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 98
BspTI CTTAAG 3 cut(s) 140, 338, 835
BspTNI GGTCTC 1 cut(s) 959
BsrFI RCCGGY 3 cut(s) 101, 162, 1180
BsrI ACTGG 6 cut(s) 116, 329, 500, 509, 1122, 1219
BssAI RCCGGY 3 cut(s) 101, 162, 1180
BssECI CCNNGG 4 cut(s) 47, 655, 1237, 1238
BssMI GATC 5 cut(s) 114, 132, 422, 481, 970
BssT1I CCWWGG 2 cut(s) 47, 655
Bst2UI CCWGG 3 cut(s) 294, 915, 1239
Bst4CI ACNGT 4 cut(s) 446, 529, 713, 845
Bst6I CTCTTC 2 cut(s) 32, 149
BstAFI CTTAAG 3 cut(s) 140, 338, 835
BstC8I GCNNGC 4 cut(s) 164, 381, 516, 1182
BstDEI CTNAG 4 cut(s) 167, 578, 741, 1144
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 2 cut(s) 51, 1232
BstF5I GGATG 1 cut(s) 757
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 5 cut(s) 117, 135, 425, 484, 973
BstMAI GTCTC 3 cut(s) 632, 788, 959
BstMBI GATC 5 cut(s) 114, 132, 422, 481, 970
BstMWI GCNNNNNNNGC 5 cut(s) 470, 511, 595, 728, 731
BstNI CCWGG 3 cut(s) 294, 915, 1239
BstNSI RCATGY 2 cut(s) 778, 940
BstPI GGTNACC 2 cut(s) 51, 1232
BstSCI CCNGG 3 cut(s) 292, 913, 1237
BstV2I GAAGAC 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 1202
BsuRI GGCC 5 cut(s) 396, 499, 664, 1136, 1184
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 757
BtsIMutI CAGTG 2 cut(s) 516, 850
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 164, 381, 516, 1182
CciI TCATGA 2 cut(s) 634, 1156
CfoI GCGC 1 cut(s) 366
Cfr10I RCCGGY 3 cut(s) 101, 162, 1180
Cfr13I GGNCC 4 cut(s) 44, 663, 911, 1168
CsiI ACCWGGT 1 cut(s) 913
Csp6I GTAC 2 cut(s) 447, 530
CviQI GTAC 2 cut(s) 447, 530
DdeI CTNAG 4 cut(s) 167, 578, 741, 1144
DpnI GATC 5 cut(s) 116, 134, 424, 483, 972
DpnII GATC 5 cut(s) 114, 132, 422, 481, 970
EaeI YGGCCR 2 cut(s) 497, 1182
Eam1104I CTCTTC 2 cut(s) 32, 149
EarI CTCTTC 2 cut(s) 32, 149
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 47, 655
Eco147I AGGCCT 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 959
Eco32I GATATC 1 cut(s) 1124
Eco47I GGWCC 3 cut(s) 44, 911, 1168
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 78, 237, 998
Eco91I GGTNACC 2 cut(s) 51, 1232
EcoICRI GAGCTC 1 cut(s) 11
EcoO65I GGTNACC 2 cut(s) 51, 1232
EcoRII CCWGG 3 cut(s) 292, 913, 1237
EcoRV GATATC 1 cut(s) 1124
EcoT14I CCWWGG 2 cut(s) 47, 655
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 2 cut(s) 47, 655
FokI GGATG 1 cut(s) 764
FriOI GRGCYC 1 cut(s) 13
FspBI CTAG 2 cut(s) 398, 1080
GlaI GCGC 1 cut(s) 365
GsaI CCCAGC 1 cut(s) 1194
HaeIII GGCC 5 cut(s) 396, 499, 664, 1136, 1184
HapII CCGG 4 cut(s) 102, 163, 500, 1181
HhaI GCGC 1 cut(s) 366
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HindIII AAGCTT 2 cut(s) 347, 596
HinfI GANTC 1 cut(s) 805
HpaII CCGG 4 cut(s) 102, 163, 500, 1181
HphI GGTGA 3 cut(s) 45, 968, 1121
Hpy188I TCNGA 4 cut(s) 217, 581, 619, 1147
Hpy188III TCNNGA 3 cut(s) 635, 695, 1157
HpyAV CCTTC 3 cut(s) 564, 840, 973
HpyCH4III ACNGT 4 cut(s) 446, 529, 713, 845
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 5 cut(s) 240, 281, 518, 778, 872
HpyF10VI GCNNNNNNNGC 5 cut(s) 470, 511, 595, 728, 731
HpyF3I CTNAG 4 cut(s) 167, 578, 741, 1144
HpySE526I ACGT 1 cut(s) 33
HspAI GCGC 1 cut(s) 364
KroI GCCGGC 2 cut(s) 162, 1180
KroNI GCCGGC 2 cut(s) 164, 1182
Kzo9I GATC 5 cut(s) 114, 132, 422, 481, 970
LguI GCTCTTC 1 cut(s) 149
LmnI GCTCC 2 cut(s) 16, 502
LweI GCATC 3 cut(s) 55, 576, 1167
MabI ACCWGGT 1 cut(s) 913
MaeI CTAG 2 cut(s) 398, 1080
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 4 cut(s) 20, 51, 107, 1232
MalI GATC 5 cut(s) 116, 134, 424, 483, 972
MboI GATC 5 cut(s) 114, 132, 422, 481, 970
MhlI GDGCHC 2 cut(s) 13, 759
MluCI AATT 6 cut(s) 178, 234, 276, 789, 820, 866
MmeI TCCRAC 1 cut(s) 1011
MroNI GCCGGC 2 cut(s) 162, 1180
MseI TTAA 8 cut(s) 141, 176, 339, 402, 561, 624, 788, 836
MslI CAYNNNNRTG 1 cut(s) 633
MspA1I CMGCKG 3 cut(s) 379, 725, 976
MspCI CTTAAG 3 cut(s) 140, 338, 835
MspI CCGG 4 cut(s) 102, 163, 500, 1181
MspR9I CCNGG 3 cut(s) 294, 915, 1239
MvaI CCWGG 3 cut(s) 294, 915, 1239
MwoI GCNNNNNNNGC 5 cut(s) 470, 511, 595, 728, 731
NaeI GCCGGC 2 cut(s) 164, 1182
NcoI CCATGG 1 cut(s) 47
NdeII GATC 5 cut(s) 114, 132, 422, 481, 970
NgoMIV GCCGGC 2 cut(s) 162, 1180
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 2 cut(s) 20, 51
NspI RCATGY 2 cut(s) 778, 940
PagI TCATGA 2 cut(s) 634, 1156
PaqCI CACCTGC 1 cut(s) 255
PasI CCCWGGG 1 cut(s) 1238
PceI AGGCCT 1 cut(s) 396
PciI ACATGT 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 149
PdiI GCCGGC 2 cut(s) 164, 1182
PfeI GAWTC 1 cut(s) 805
PscI ACATGT 1 cut(s) 936
PshBI ATTAAT 1 cut(s) 624
PsiI TTATAA 1 cut(s) 1252
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 3 cut(s) 292, 913, 1237
PspEI GGTNACC 2 cut(s) 51, 1232
PspFI CCCAGC 1 cut(s) 1190
PspGI CCWGG 3 cut(s) 292, 913, 1237
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 4 cut(s) 44, 663, 911, 1168
PvuII CAGCTG 2 cut(s) 379, 976
RsaI GTAC 2 cut(s) 448, 531
RsaNI GTAC 2 cut(s) 447, 530
RseI CAYNNNNRTG 1 cut(s) 633
SacI GAGCTC 1 cut(s) 13
SapI GCTCTTC 1 cut(s) 149
SaqAI TTAA 8 cut(s) 141, 176, 339, 402, 561, 624, 788, 836
Sau3AI GATC 5 cut(s) 114, 132, 422, 481, 970
Sau96I GGNCC 4 cut(s) 44, 663, 911, 1168
ScaI AGTACT 2 cut(s) 448, 531
ScrFI CCNGG 3 cut(s) 294, 915, 1239
SduI GDGCHC 2 cut(s) 13, 759
SexAI ACCWGGT 1 cut(s) 913
SfaNI GCATC 3 cut(s) 55, 576, 1167
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 3 cut(s) 44, 911, 1168
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 4 cut(s) 74, 140, 338, 835
SmoI CTYRAG 4 cut(s) 74, 140, 338, 835
SpeI ACTAGT 1 cut(s) 1079
Sse9I AATT 6 cut(s) 178, 234, 276, 789, 820, 866
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 6 cut(s) 105, 187, 429, 723, 989, 1044
SspI AATATT 1 cut(s) 673
SspMI CTAG 2 cut(s) 398, 1080
SstI GAGCTC 1 cut(s) 13
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 3 cut(s) 292, 913, 1237
StyI CCWWGG 2 cut(s) 47, 655
TaaI ACNGT 4 cut(s) 446, 529, 713, 845
TaiI ACGT 1 cut(s) 36
TaqI TCGA 2 cut(s) 87, 796
TaqII GACCGA 2 cut(s) 983, 1185
TasI AATT 6 cut(s) 178, 234, 276, 789, 820, 866
TatI WGTACW 2 cut(s) 446, 529
TauI GCSGC 3 cut(s) 432, 725, 1047
TfiI GAWTC 1 cut(s) 805
Tru1I TTAA 8 cut(s) 141, 176, 339, 402, 561, 624, 788, 836
Tru9I TTAA 8 cut(s) 141, 176, 339, 402, 561, 624, 788, 836
TscAI CASTG 2 cut(s) 516, 850
TseFI GTSAC 2 cut(s) 20, 51
Tsp45I GTSAC 2 cut(s) 20, 51
TspDTI ATGAA 4 cut(s) 705, 797, 944, 1079
TspRI CASTG 2 cut(s) 516, 850
Vha464I CTTAAG 3 cut(s) 140, 338, 835
VpaK11BI GGWCC 3 cut(s) 44, 911, 1168
VspI ATTAAT 1 cut(s) 624
XceI RCATGY 2 cut(s) 778, 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 1226
XspI CTAG 2 cut(s) 398, 1080
ZrmI AGTACT 2 cut(s) 448, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.