MD08G1084100.v1.1

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003679
Physical Location & Seq
Reverse (-)
6965434 .. 6967518
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1084100.v1.1.491

Sequence Viewer

Length: 2085 bp
ATGAAGGCGATGAAGCAAAAGCTGGTACTTACAGTGGAACCAGAATTCTGCTCCTCAGCAGTTCAATACTGGGCGAATGGCAACACGAAGCGCGGACGCGGGAAGTCCGACGTCACTGAGAAGGCGAAAAGGCACCTCTCCGTCTTGGGATGGAAGTTCTGGTATGCTAACAAGCAGCAGAAGACCGAGTTGCGGTACGAGTCTCCCATCGGAAAAGTCTACTACTCGCTACGCCTGGCTTGCCAAGCCTGTGTAAATTCTCCAGGAGGAGGAGTGTTTTCCGAAAGCACTACTTCAAATTCCGTGGTGTCCGTGGAGTGCTCTGCTGCTCCTGATATCGTAGTAGATGTTATGCCGCAATCGACATCAAGAAAAACGGTTAAGAAGAGAAAGATTGGTGAAGCATCGAAGGTTGATGATGACTGGAGTCCGAAACAGAAGCGAGGTAAGGTTCTTGCGGATATGAAGAGACTAAGGAAACAGAATAAAGCTACTAAACAGCAGGTCACACGCCTTTTACGGTCTACCAAAACAGCGAGAGAGATTGAGATAACGGATCCTGGTACTCGAAACCCTAGGACAGTCCTCTCTTGGTTGATAGACAGTAATGCTGTGTCGCCGAATGCTAAGGTACACTACCGCCCTAGAAGAGGCACTGACAGTCCATTGGCAATCGGTCAGATAACCCGCGAGGGAATCAAGTGTGACTGTTGTTCTAAGGTGTTTACCCTCACTGGATTTGAAACACATGCTGGTAGCACAAACCATAGACCAAGCGCCCACATTATTTTGGAAGATGGCAGGACTCTCAACGATTGTCAGAGGCAGATGATGAAGAGTCGGAAAGGAAGCACCAACACTGTCACACGGTCAAATGACGACGCGAGGGCTGACAATGTGCGTCAAGATCATCATCATCATCATGAGCAGAATGATGATATATGCACTGTGTGTCACTTTGGAGGCGATCTGATTCTGTGTGATCGGTGTCCTGCTGCATTCCACTCTAGTTGTCTCGATTTGAAGGACGTGTTGGAAGGCAATTGGTTCTGCCCATCGTGTTGCTGTGTGAAGTGTGGTAAAGGAAACCCTAAGGAGGATAGAAATAATGGTTTTGTGATATGTAGCCAATGTGATAAAAAATACCACTTTGGGTGCCTGAGGAATGAAGGGGTTGCGGAATTGGAAAGGGATTCCAAAGGAAACTGGTTTTGCAGCAGAAAATGTGAAGGCATGTATAAGGGAATCAACAAGATTGTGGGGAAACGAATTCTGGTGGGTCCCGACAATCTGACGTGGACAATATTGAGGCCATCTACTCCGTCTGATTCTGACATGGAAGACTTGACAGAGAACTACAGCAAGCTCAATTTGGCCCTGAGTGTGATGCACGAGTGTTTCGAGCCTTCTAAGGATCCTTACACGAAAAGAGACATTGTCGAGGACATTATTTTCAACAGAGAATCGGACCTTAGCCGCCTGAACTTCAGAAGGTTTTACACAGTGCTTTTGGAGAGAGATGAGGAAGTGATTACCGTCGGTAGCGTGAGGATATACAGTGAGTTGGCGGAAGTACCTCTGGTGGCAACTAGGTTCCATTATCGTAGGCAAGGAATGTGTCATGTTATGATGGATGAGCTCGAAAACCAGCTTGTCAACTTGGGAGTTGGGAGATTGATTTTGCCTTCTGCTTCTAGCACACTGGATACATGGACCAGCACTTCATTTGGGTTTTCAAAGATGACAGCTAATGAGAGGATGCAGTTTCTGAATTATACTTTCATGGATTTCCAGGACACCATCTTGTGCCATAAAGTATTGAAAAGGACCAACTCTACGACGCCAGCAACCATACCGTTTGAAGGAAGTATCAAATTTGATGGATCGCCCAGTGCCACCTCTACAGTAACTCAGGCAGAAGACAATCAGCCGGATAATGGAGCTTCAGACCAAGAATTGGGGAACGTTGCCTCCGGTGACAAGTTTTTGATTGATGTTGACTGCATGCTGTTGGACAACAACGAACCTAGTTTCTCGGCATGGTACAACAAACAGAATTTTAGGTTGATTCCAGGATTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

695

Amino Acids

78.22

Weight (kDa)

8.58

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 11 - 86 6.9e-27 Domain of unknown function (DUF7028)
TDBD PF16135 226 - 277 6.1e-23 Tify domain binding domain
PHD PF00628 315 - 354 1e-07 PHD-finger
IDM1_C PF23209 467 - 605 3.9e-47 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
Acc36I ACCTGC 1 cut(s) 493
AccB1I GGYRCC 2 cut(s) 132, 1155
AccB7I CCANNNNNTGG 1 cut(s) 1957
AccI GTMKAC 2 cut(s) 219, 524
AccII CGCG 4 cut(s) 93, 99, 690, 884
AclI AACGTT 1 cut(s) 1965
AclWI GGATC 5 cut(s) 551, 564, 1409, 1422, 1891
AcsI RAATTY 6 cut(s) 44, 256, 298, 1269, 1874, 2056
AcuI CTGAAG 2 cut(s) 1471, 1929
AcyI GRCGYC 2 cut(s) 111, 1841
AdeI CACNNNGTG 1 cut(s) 951
AfaI GTAC 6 cut(s) 27, 197, 565, 633, 1575, 2045
AfiI CCNNNNNNNGG 7 cut(s) 192, 269, 650, 1096, 1862, 1937, 1957
AflIII ACRYGT 1 cut(s) 1029
AgsI TTSAA 8 cut(s) 65, 297, 743, 1024, 1456, 1737, 1822, 1862
AjiI CACGTC 2 cut(s) 1030, 1296
AjnI CCWGG 5 cut(s) 234, 262, 559, 1791, 2071
AjuI GAANNNNNNNTTGG 2 cut(s) 1016, 1048
AloI GAACNNNNNNTCC 2 cut(s) 1955, 1987
AluBI AGCT 7 cut(s) 22, 491, 1366, 1639, 1651, 1748, 1943
AluI AGCT 7 cut(s) 22, 491, 1366, 1639, 1651, 1748, 1943
Alw21I GWGCWC 2 cut(s) 323, 1641
Alw26I GTCTC 4 cut(s) 207, 463, 1019, 1425
AlwI GGATC 5 cut(s) 551, 564, 1409, 1422, 1891
AlwNI CAGNNNCTG 1 cut(s) 1768
AoxI GGCC 2 cut(s) 1310, 1374
ApeKI GCWGC 4 cut(s) 175, 326, 995, 1215
ApoI RAATTY 6 cut(s) 44, 256, 298, 1269, 1874, 2056
AspA2I CCTAGG 1 cut(s) 575
AspLEI GCGC 2 cut(s) 93, 779
AspS9I GGNCC 5 cut(s) 1280, 1375, 1468, 1713, 1827
AsuHPI GGTGA 2 cut(s) 410, 1988
AvaII GGWCC 4 cut(s) 1280, 1468, 1713, 1827
AvrII CCTAGG 1 cut(s) 575
AxyI CCTNAGG 2 cut(s) 1092, 1160
BamHI GGATCC 2 cut(s) 556, 1414
BanI GGYRCC 2 cut(s) 132, 1155
BanII GRGCYC 1 cut(s) 1641
BauI CACGAG 1 cut(s) 1391
BbsI GAAGAC 3 cut(s) 188, 1347, 1926
Bbv12I GWGCWC 2 cut(s) 323, 1641
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 4 cut(s) 187, 313, 982, 1227
BccI CCATC 8 cut(s) 144, 215, 791, 1063, 1321, 1624, 1808, 1874
BciT130I CCWGG 5 cut(s) 236, 264, 561, 1793, 2073
BciVI GTATCC 1 cut(s) 1699
BcoDI GTCTC 4 cut(s) 207, 463, 1019, 1425
BfaI CTAG 6 cut(s) 576, 645, 1008, 1590, 1695, 2028
BfmI CTRYAG 2 cut(s) 1357, 1902
BfoI RGCGCY 1 cut(s) 780
BfuAI ACCTGC 1 cut(s) 493
BfuI GTATCC 1 cut(s) 1699
BisI GCNGC 6 cut(s) 176, 327, 356, 996, 1216, 1477
BlnI CCTAGG 1 cut(s) 575
BlsI GCNGC 6 cut(s) 177, 328, 357, 997, 1217, 1478
Bme1390I CCNGG 5 cut(s) 236, 264, 561, 1793, 2073
Bme18I GGWCC 4 cut(s) 1280, 1468, 1713, 1827
BmgBI CACGTC 2 cut(s) 1030, 1296
BmgT120I GGNCC 5 cut(s) 1280, 1375, 1468, 1713, 1827
BmiI GGNNCC 8 cut(s) 39, 134, 558, 1157, 1281, 1282, 1416, 1595
BmrFI CCNGG 5 cut(s) 236, 264, 561, 1793, 2073
BmrI ACTGGG 2 cut(s) 79, 1884
BmsI GCATC 3 cut(s) 413, 1377, 1749
BmuI ACTGGG 2 cut(s) 79, 1884
BoxI GACNNNNGTC 1 cut(s) 426
BpiI GAAGAC 3 cut(s) 188, 1347, 1926
BpmI CTGGAG 2 cut(s) 246, 445
Bpu10I CCTNAGC 3 cut(s) 55, 627, 1472
BsaBI GATNNNNATC 1 cut(s) 912
BsaHI GRCGYC 2 cut(s) 111, 1841
BsaJI CCNNGG 3 cut(s) 303, 312, 575
BsaWI WCCGGW 1 cut(s) 1973
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 1955, 1985
Bsc4I CCNNNNNNNGG 7 cut(s) 192, 269, 650, 1096, 1862, 1937, 1957
Bse1I ACTGG 6 cut(s) 74, 428, 739, 1211, 1707, 1890
Bse21I CCTNAGG 2 cut(s) 1092, 1160
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 5 cut(s) 236, 264, 561, 1793, 2073
BseDI CCNNGG 3 cut(s) 303, 312, 575
BseGI GGATG 3 cut(s) 155, 1639, 1764
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 7 cut(s) 192, 269, 650, 1096, 1862, 1937, 1957
BseMII CTCAG 5 cut(s) 69, 108, 1151, 1370, 1925
BseNI ACTGG 6 cut(s) 74, 428, 739, 1211, 1707, 1890
BseRI GAGGAG 3 cut(s) 43, 282, 285
BseXI GCAGC 4 cut(s) 187, 313, 982, 1227
Bsh1236I CGCG 4 cut(s) 93, 99, 690, 884
BshFI GGCC 2 cut(s) 1312, 1376
BshNI GGYRCC 2 cut(s) 132, 1155
BsiHKAI GWGCWC 2 cut(s) 323, 1641
BsiSI CCGG 2 cut(s) 1931, 1974
BslFI GGGAC 1 cut(s) 1266
BslI CCNNNNNNNGG 7 cut(s) 192, 269, 650, 1096, 1862, 1937, 1957
BsmAI GTCTC 4 cut(s) 207, 463, 1019, 1425
BsmFI GGGAC 1 cut(s) 1266
BsmI GAATGC 2 cut(s) 628, 998
BsnI GGCC 2 cut(s) 1312, 1376
Bsp1286I GDGCHC 2 cut(s) 323, 1641
Bsp143I GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
BspANI GGCC 2 cut(s) 1312, 1376
BspCNI CTCAG 5 cut(s) 68, 109, 1152, 1371, 1924
BspFNI CGCG 4 cut(s) 93, 99, 690, 884
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 8 cut(s) 39, 134, 558, 1157, 1281, 1282, 1416, 1595
BspMI ACCTGC 1 cut(s) 493
BspPI GGATC 5 cut(s) 551, 564, 1409, 1422, 1891
BspT107I GGYRCC 2 cut(s) 132, 1155
BsrI ACTGG 6 cut(s) 74, 428, 739, 1211, 1707, 1890
BssECI CCNNGG 3 cut(s) 303, 312, 575
BssMI GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
BssNI GRCGYC 2 cut(s) 111, 1841
BssSI CACGAG 1 cut(s) 1391
BssT1I CCWWGG 1 cut(s) 575
Bst2BI CACGAG 1 cut(s) 1391
Bst2UI CCWGG 5 cut(s) 236, 264, 561, 1793, 2073
Bst6I CTCTTC 4 cut(s) 380, 461, 643, 830
BstACI GRCGYC 2 cut(s) 111, 1841
BstC8I GCNNGC 4 cut(s) 241, 1364, 1845, 2006
BstDSI CCRYGG 2 cut(s) 303, 312
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 3 cut(s) 155, 1639, 1764
BstFNI CGCG 4 cut(s) 93, 99, 690, 884
BstH2I RGCGCY 1 cut(s) 780
BstHHI GCGC 2 cut(s) 93, 779
BstKTI GATC 6 cut(s) 559, 910, 970, 985, 1417, 1886
BstMAI GTCTC 4 cut(s) 207, 463, 1019, 1425
BstMBI GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
BstMWI GCNNNNNNNGC 2 cut(s) 240, 245
BstNI CCWGG 5 cut(s) 236, 264, 561, 1793, 2073
BstNSI RCATGY 3 cut(s) 752, 1237, 2008
BstPAI GACNNNNGTC 1 cut(s) 426
BstSCI CCNGG 5 cut(s) 234, 262, 559, 1791, 2071
BstSFI CTRYAG 2 cut(s) 1357, 1902
BstUI CGCG 4 cut(s) 93, 99, 690, 884
BstV1I GCAGC 4 cut(s) 187, 313, 982, 1227
BstV2I GAAGAC 3 cut(s) 188, 1347, 1926
BstX2I RGATCY 2 cut(s) 556, 1414
BstYI RGATCY 2 cut(s) 556, 1414
Bsu36I CCTNAGG 2 cut(s) 1092, 1160
BsuI GTATCC 1 cut(s) 1699
BsuRI GGCC 2 cut(s) 1312, 1376
BtgI CCRYGG 2 cut(s) 303, 312
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 2 cut(s) 1030, 1296
BtsCI GGATG 3 cut(s) 155, 1639, 1764
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 4 cut(s) 241, 1364, 1845, 2006
CaiI CAGNNNCTG 1 cut(s) 1768
CciI TCATGA 1 cut(s) 922
CfoI GCGC 2 cut(s) 93, 779
Cfr13I GGNCC 5 cut(s) 1280, 1375, 1468, 1713, 1827
CseI GACGC 4 cut(s) 105, 890, 890, 1849
Csp6I GTAC 6 cut(s) 26, 196, 564, 632, 1574, 2044
CspCI CAANNNNNGTGG 4 cut(s) 285, 320, 992, 1027
CviAII CATG 9 cut(s) 749, 923, 1234, 1336, 1622, 1710, 1783, 2005, 2040
CviQI GTAC 6 cut(s) 26, 196, 564, 632, 1574, 2044
DpnI GATC 6 cut(s) 558, 909, 969, 984, 1416, 1885
DpnII GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
DraIII CACNNNGTG 1 cut(s) 951
Eam1104I CTCTTC 4 cut(s) 380, 461, 643, 830
EarI CTCTTC 4 cut(s) 380, 461, 643, 830
EciI GGCGGA 1 cut(s) 1583
Ecl136II GAGCTC 1 cut(s) 1639
Eco130I CCWWGG 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 1641
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 4 cut(s) 1280, 1468, 1713, 1827
Eco53kI GAGCTC 1 cut(s) 1639
Eco57I CTGAAG 2 cut(s) 1471, 1929
Eco81I CCTNAGG 2 cut(s) 1092, 1160
EcoICRI GAGCTC 1 cut(s) 1639
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRI GAATTC 2 cut(s) 44, 1269
EcoRII CCWGG 5 cut(s) 234, 262, 559, 1791, 2071
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 1641
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 9 cut(s) 752, 926, 1237, 1339, 1625, 1713, 1786, 2008, 2043
FalI AAGNNNNNCTT 2 cut(s) 277, 309
FaqI GGGAC 1 cut(s) 1266
FatI CATG 9 cut(s) 748, 922, 1233, 1335, 1621, 1709, 1782, 2004, 2039
FauI CCCGC 2 cut(s) 92, 695
FblI GTMKAC 2 cut(s) 219, 524
Fnu4HI GCNGC 6 cut(s) 176, 327, 356, 996, 1216, 1477
FokI GGATG 3 cut(s) 162, 1646, 1771
FriOI GRGCYC 1 cut(s) 1641
Fsp4HI GCNGC 6 cut(s) 176, 327, 356, 996, 1216, 1477
FspBI CTAG 6 cut(s) 576, 645, 1008, 1590, 1695, 2028
GlaI GCGC 2 cut(s) 92, 778
GluI GCNGC 6 cut(s) 176, 327, 356, 996, 1216, 1477
GsuI CTGGAG 2 cut(s) 246, 445
HaeII RGCGCY 1 cut(s) 780
HaeIII GGCC 2 cut(s) 1312, 1376
HapII CCGG 2 cut(s) 1931, 1974
HgaI GACGC 4 cut(s) 105, 890, 890, 1849
HhaI GCGC 2 cut(s) 93, 779
Hin1I GRCGYC 2 cut(s) 111, 1841
Hin1II CATG 9 cut(s) 752, 926, 1237, 1339, 1625, 1713, 1786, 2008, 2043
Hin6I GCGC 2 cut(s) 91, 777
HinP1I GCGC 2 cut(s) 91, 777
HincII GTYRAC 2 cut(s) 1657, 1999
HindII GTYRAC 2 cut(s) 1657, 1999
HpaII CCGG 2 cut(s) 1931, 1974
HphI GGTGA 2 cut(s) 410, 1988
Hpy166II GTNNAC 7 cut(s) 220, 525, 634, 726, 1299, 1657, 1999
Hpy188III TCNNGA 6 cut(s) 332, 369, 905, 923, 1016, 1283
Hpy8I GTNNAC 7 cut(s) 220, 525, 634, 726, 1299, 1657, 1999
Hpy99I CGWCG 4 cut(s) 113, 884, 1541, 1843
HpyCH4IV ACGT 4 cut(s) 111, 1029, 1295, 1965
HpyCH4V TGCA 6 cut(s) 945, 998, 1215, 1390, 1762, 2004
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 245
HpySE526I ACGT 4 cut(s) 111, 1029, 1295, 1965
Hsp92I GRCGYC 2 cut(s) 111, 1841
Hsp92II CATG 9 cut(s) 752, 926, 1237, 1339, 1625, 1713, 1786, 2008, 2043
HspAI GCGC 2 cut(s) 91, 777
KflI GGGWCCC 1 cut(s) 1280
Kzo9I GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
LmnI GCTCC 3 cut(s) 56, 334, 1940
Lsp1109I GCAGC 4 cut(s) 187, 313, 982, 1227
LweI GCATC 3 cut(s) 413, 1377, 1749
MaeI CTAG 6 cut(s) 576, 645, 1008, 1590, 1695, 2028
MaeII ACGT 4 cut(s) 111, 1029, 1295, 1965
MaeIII GTNAC 7 cut(s) 112, 505, 704, 862, 953, 1906, 1976
MalI GATC 6 cut(s) 558, 909, 969, 984, 1416, 1885
MboI GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
MboII GAAGA 8 cut(s) 193, 397, 478, 660, 806, 847, 1352, 1931
MfeI CAATTG 1 cut(s) 1042
MflI RGATCY 2 cut(s) 556, 1414
MhlI GDGCHC 2 cut(s) 323, 1641
MlyI GAGTC 4 cut(s) 209, 436, 799, 847
MmeI TCCRAC 4 cut(s) 132, 821, 1014, 1992
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 921
MspI CCGG 2 cut(s) 1931, 1974
MspR9I CCNGG 5 cut(s) 236, 264, 561, 1793, 2073
MunI CAATTG 1 cut(s) 1042
Mva1269I GAATGC 2 cut(s) 628, 998
MvaI CCWGG 5 cut(s) 236, 264, 561, 1793, 2073
MvnI CGCG 4 cut(s) 93, 99, 690, 884
MwoI GCNNNNNNNGC 2 cut(s) 240, 245
NdeII GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
NlaIII CATG 9 cut(s) 752, 926, 1237, 1339, 1625, 1713, 1786, 2008, 2043
NlaIV GGNNCC 8 cut(s) 39, 134, 558, 1157, 1281, 1282, 1416, 1595
NmeAIII GCCGAG 1 cut(s) 2015
NmuCI GTSAC 6 cut(s) 112, 505, 704, 862, 953, 1976
NspI RCATGY 3 cut(s) 752, 1237, 2008
PaeI GCATGC 1 cut(s) 2008
PagI TCATGA 1 cut(s) 922
PcsI WCGNNNNNNNCGW 1 cut(s) 1398
PctI GAATGC 2 cut(s) 628, 998
PfeI GAWTC 7 cut(s) 696, 973, 1193, 1245, 1328, 1463, 2068
PflFI GACNNNGTC 1 cut(s) 1436
PflMI CCANNNNNTGG 1 cut(s) 1957
PfoI TCCNGGA 3 cut(s) 262, 1791, 2071
PkrI GCNGC 6 cut(s) 177, 328, 357, 997, 1217, 1478
PleI GAGTC 4 cut(s) 208, 435, 799, 846
PpsI GAGTC 4 cut(s) 208, 435, 799, 846
PpuMI RGGWCCY 1 cut(s) 1280
PshAI GACNNNNGTC 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 1641
Psp1406I AACGTT 1 cut(s) 1965
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 5 cut(s) 234, 262, 559, 1791, 2071
PspGI CCWGG 5 cut(s) 234, 262, 559, 1791, 2071
PspN4I GGNNCC 8 cut(s) 39, 134, 558, 1157, 1281, 1282, 1416, 1595
PspPI GGNCC 5 cut(s) 1280, 1375, 1468, 1713, 1827
PspPPI RGGWCCY 1 cut(s) 1280
PstNI CAGNNNCTG 1 cut(s) 1768
PsuI RGATCY 2 cut(s) 556, 1414
PsyI GACNNNGTC 1 cut(s) 1436
RsaI GTAC 6 cut(s) 27, 197, 565, 633, 1575, 2045
RsaNI GTAC 6 cut(s) 26, 196, 564, 632, 1574, 2044
RseI CAYNNNNRTG 1 cut(s) 921
SacI GAGCTC 1 cut(s) 1641
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 6 cut(s) 176, 327, 356, 996, 1216, 1477
Sau3AI GATC 6 cut(s) 556, 907, 967, 982, 1414, 1883
Sau96I GGNCC 5 cut(s) 1280, 1375, 1468, 1713, 1827
SchI GAGTC 4 cut(s) 209, 436, 799, 847
ScrFI CCNGG 5 cut(s) 236, 264, 561, 1793, 2073
SduI GDGCHC 2 cut(s) 323, 1641
SfaNI GCATC 3 cut(s) 413, 1377, 1749
SfcI CTRYAG 2 cut(s) 1357, 1902
SinI GGWCC 4 cut(s) 1280, 1468, 1713, 1827
SmiMI CAYNNNNRTG 1 cut(s) 921
SphI GCATGC 1 cut(s) 2008
SspI AATATT 1 cut(s) 1305
SspMI CTAG 6 cut(s) 576, 645, 1008, 1590, 1695, 2028
SstI GAGCTC 1 cut(s) 1641
StyD4I CCNGG 5 cut(s) 234, 262, 559, 1791, 2071
StyI CCWWGG 1 cut(s) 575
TaiI ACGT 4 cut(s) 114, 1032, 1298, 1968
TaqI TCGA 7 cut(s) 362, 407, 568, 1017, 1401, 1440, 1641
TaqII GACCGA 2 cut(s) 200, 665
TauI GCSGC 2 cut(s) 358, 1479
TfiI GAWTC 7 cut(s) 696, 973, 1193, 1245, 1328, 1463, 2068
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseFI GTSAC 6 cut(s) 112, 505, 704, 862, 953, 1976
TseI GCWGC 4 cut(s) 175, 326, 995, 1215
Tsp45I GTSAC 6 cut(s) 112, 505, 704, 862, 953, 1976
TspDTI ATGAA 7 cut(s) 17, 26, 479, 848, 1182, 1713, 1771
TspGWI ACGGA 5 cut(s) 130, 292, 301, 569, 1311
Tth111I GACNNNGTC 1 cut(s) 1436
Van91I CCANNNNNTGG 1 cut(s) 1957
VpaK11BI GGWCC 4 cut(s) 1280, 1468, 1713, 1827
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 6 cut(s) 44, 256, 298, 1269, 1874, 2056
XceI RCATGY 3 cut(s) 752, 1237, 2008
XmaJI CCTAGG 1 cut(s) 575
XmiI GTMKAC 2 cut(s) 219, 524
XspI CTAG 6 cut(s) 576, 645, 1008, 1590, 1695, 2028
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.