MD15G1070200.v1.1

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003679
Physical Location & Seq
Reverse (-)
4794609 .. 4795971
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1070200.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGAAGGCTGTGAAGCGAAAGCCGGTACTTACCGTGGAACCAGAGTTATGCCCCTCGGCAGTTCAGTACTGGGCGAATGACAACACGAAGAACGGCCGTAGGAACTCTGATATTACCGAGAAGGCGAGAAGGCACCTCTCCGCCTTGGGATGGAAGTTCTGGTATGCAACCAAGAAGCAGAAGATCGAGTTGCGGTACGAGTCTCCCACTGGAAAAGTTTACTACTCGCTTCGCATGGCTTGCCAAGCCTGTGTAAATTCTCCAGGACGAGGAGGAGTGTTTTCCGAAAGCATTGGTTCGAATTCCGTGGAGTGCTCTGCTGATCTTGATATTGTAGTAGATATTACACCGCAGTCGACATCAAGGAAAACGTTTAAGAAGAGAGAGATTGGTGAAATCTCGAAGGTAGATGATGATTGGAGTCCGAAACAGGAGCGGGGGAAAACAGTTAAGAAGAGAAAGATTGGTGGAATATCGATGGTCGATGATGATTGGAGTCCGGAACAGAAGCGAGGTAAGGTTCATGCGGATATGAAGAGACTAAAGAGACAGAATAAAGCTAGTAAACAGCAGGCCAGACGCATTTTACGGTCAACCAAAAGAGCGAGAGATATCGAGATCACGGATCCCGGTACTCGAAACCCTAGGACAGTCCTGTCTTGGTTGATCGAAAGTAATGGTGTGTCCCCGAACGCCAAGGTACACTACCGCCCTAGAAAGGGCACTGACAGTCCATTGGCGACGGGTCGGATTACTCGCGAGGGAATCAAGTGTGACTGTTGTTTTAAGGTGTTTACCCTCACTGGGTTTGAAACGCACGCTGGTAGCACAAACCATCGACCAAGCGCCCACATTATATTGGAAGATGGCAGGACTCTCAACGATTGTCAGAGGCAAACGATGAAGAGTCGGAAAGGAAGCGCCAACACCGTCACGAGGTCAAATGAAGTAGCGACGACTGGCAATGTGCATCAAGATCATCATCCTCCTCGTGATCAGAACGATGATATATGCACTGTGTGTCACTTTGGAGGCGATCTGATTCTCTGTGATCGGTGTCCCGCTGCATTCCACACTAGTTGCCTTGGTTTGAAGGATGTGTCGGAAGGCGATTGGTTCTGCCCATCGTGTTGCTGTGTAAAGTGTGGAATGAAGGGGTTGAGGAATTGGAAAGGGATTCCAAAGGAAACTGGTTTTGCAGCAGAAAATGTGAAGGCATATATCTGGGTATCAACAAGATTGTGGGGAAACGAATTCTGGTGGGTACTGACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

426

Amino Acids

47.94

Weight (kDa)

9.52

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 11 - 86 4.4e-29 Domain of unknown function (DUF7028)
TDBD PF16135 249 - 299 1e-22 Tify domain binding domain
PHD-1st_NSD PF23011 334 - 377 1.3e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 338 - 377 2e-08 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 655
AccB1I GGYRCC 1 cut(s) 132
AccBSI CCGCTC 1 cut(s) 436
AccI GTMKAC 1 cut(s) 356
AccII CGCG 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 7 cut(s) 141, 193, 350, 436, 527, 709, 1062
AclI AACGTT 1 cut(s) 371
AclWI GGATC 2 cut(s) 620, 633
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 3 cut(s) 256, 301, 1253
AdeI CACNNNGTG 1 cut(s) 1020
AfaI GTAC 6 cut(s) 27, 68, 197, 634, 702, 1266
AfiI CCNNNNNNNGG 6 cut(s) 150, 269, 718, 719, 804, 936
AgsI TTSAA 2 cut(s) 812, 1093
AhlI ACTAGT 1 cut(s) 1076
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 1 cut(s) 560
AluI AGCT 1 cut(s) 560
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 3 cut(s) 207, 532, 541
AlwI GGATC 2 cut(s) 620, 633
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 2 cut(s) 94, 573
ApeKI GCWGC 2 cut(s) 1064, 1199
ApoI RAATTY 3 cut(s) 256, 301, 1253
AspA2I CCTAGG 1 cut(s) 644
AspLEI GCGC 2 cut(s) 848, 923
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 404
AsuII TTCGAA 1 cut(s) 299
AvrII CCTAGG 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 725
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 132
BauI CACGAG 2 cut(s) 934, 990
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 1051, 1211
BccI CCATC 5 cut(s) 144, 472, 843, 860, 1132
BceAI ACGGC 2 cut(s) 81, 109
BciT130I CCWGG 1 cut(s) 264
BclI TGATCA 1 cut(s) 994
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 3 cut(s) 207, 532, 541
BcuI ACTAGT 1 cut(s) 1076
BfaI CTAG 4 cut(s) 561, 645, 714, 1077
BfoI RGCGCY 2 cut(s) 849, 924
BisI GCNGC 2 cut(s) 1065, 1200
BlnI CCTAGG 1 cut(s) 644
BlsI GCNGC 2 cut(s) 1066, 1201
BmcAI AGTACT 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 264, 630
BmiI GGNNCC 3 cut(s) 39, 134, 627
BmrFI CCNGG 2 cut(s) 264, 630
BmrI ACTGGG 2 cut(s) 79, 813
BmsI GCATC 1 cut(s) 979
BmuI ACTGGG 2 cut(s) 79, 813
BpmI CTGGAG 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 981
BsaJI CCNNGG 7 cut(s) 33, 54, 144, 306, 644, 696, 1084
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 269, 718, 719, 804, 936
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 5 cut(s) 74, 214, 808, 964, 1195
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 981
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 1 cut(s) 264
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 7 cut(s) 33, 54, 144, 306, 644, 696, 1084
BseGI GGATG 3 cut(s) 155, 982, 1102
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 6 cut(s) 150, 269, 718, 719, 804, 936
BseMI GCAATG 1 cut(s) 970
BseNI ACTGG 5 cut(s) 74, 214, 808, 964, 1195
BseRI GAGGAG 3 cut(s) 285, 288, 978
BseSI GKGCMC 1 cut(s) 725
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 1051, 1211
Bsh1236I CGCG 1 cut(s) 759
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 2 cut(s) 96, 575
BshNI GGYRCC 1 cut(s) 132
BshVI ATCGAT 1 cut(s) 476
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 3 cut(s) 23, 500, 630
BslFI GGGAC 2 cut(s) 670, 1044
BslI CCNNNNNNNGG 6 cut(s) 150, 269, 718, 719, 804, 936
BsmAI GTCTC 3 cut(s) 207, 532, 541
BsmFI GGGAC 2 cut(s) 670, 1044
BsmI GAATGC 1 cut(s) 1067
BsnI GGCC 2 cut(s) 96, 575
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 317, 725
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
Bsp68I TCGCGA 1 cut(s) 759
BspACI CCGC 7 cut(s) 141, 193, 350, 436, 527, 709, 1062
BspANI GGCC 2 cut(s) 96, 575
BspDI ATCGAT 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 1 cut(s) 759
BspLI GGNNCC 3 cut(s) 39, 134, 627
BspPI GGATC 2 cut(s) 620, 633
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 132
BsrBI CCGCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 970
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 5 cut(s) 74, 214, 808, 964, 1195
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 7 cut(s) 33, 54, 144, 306, 644, 696, 1084
BssMI GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
BssSI CACGAG 2 cut(s) 934, 990
BssT1I CCWWGG 4 cut(s) 144, 644, 696, 1084
Bst2BI CACGAG 2 cut(s) 934, 990
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 8 cut(s) 34, 448, 591, 652, 731, 779, 931, 1018
Bst6I CTCTTC 4 cut(s) 374, 449, 530, 899
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 241, 573, 819
BstDSI CCRYGG 2 cut(s) 33, 306
BstF5I GGATG 3 cut(s) 155, 982, 1102
BstFNI CGCG 1 cut(s) 759
BstH2I RGCGCY 2 cut(s) 849, 924
BstHHI GCGC 2 cut(s) 848, 923
BstKTI GATC 9 cut(s) 186, 325, 621, 628, 669, 979, 997, 1039, 1054
BstMAI GTCTC 3 cut(s) 207, 532, 541
BstMBI GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 240, 245
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 2 cut(s) 262, 628
BstSLI GKGCMC 1 cut(s) 725
BstUI CGCG 1 cut(s) 759
BstV1I GCAGC 2 cut(s) 1051, 1211
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZI CGGCCG 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 476
BsuRI GGCC 2 cut(s) 96, 575
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 2 cut(s) 33, 306
BtsCI GGATG 3 cut(s) 155, 982, 1102
BtsIMutI CAGTG 4 cut(s) 207, 723, 801, 1014
BtuMI TCGCGA 1 cut(s) 759
Cac8I GCNNGC 3 cut(s) 241, 573, 819
CfoI GCGC 2 cut(s) 848, 923
Cfr10I RCCGGY 1 cut(s) 22
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 588
Csp6I GTAC 6 cut(s) 26, 67, 196, 633, 701, 1265
CspCI CAANNNNNGTGG 2 cut(s) 1061, 1096
CviAII CATG 2 cut(s) 235, 524
CviJI RGCY 7 cut(s) 8, 22, 96, 239, 248, 560, 575
CviKI_1 RGCY 7 cut(s) 8, 22, 96, 239, 248, 560, 575
CviQI GTAC 6 cut(s) 26, 67, 196, 633, 701, 1265
DpnI GATC 9 cut(s) 185, 324, 620, 627, 668, 978, 996, 1038, 1053
DpnII GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
DraIII CACNNNGTG 1 cut(s) 1020
DrdI GACNNNNNNGTC 1 cut(s) 655
DseDI GACNNNNNNGTC 1 cut(s) 655
EaeI YGGCCR 1 cut(s) 94
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 4 cut(s) 374, 449, 530, 899
EarI CTCTTC 4 cut(s) 374, 449, 530, 899
EciI GGCGGA 1 cut(s) 130
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 144, 644, 696, 1084
Eco32I GATATC 1 cut(s) 613
Eco52I CGGCCG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 301, 1253
EcoRII CCWGG 1 cut(s) 262
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 4 cut(s) 144, 644, 696, 1084
ErhI CCWWGG 4 cut(s) 144, 644, 696, 1084
FaeI CATG 2 cut(s) 238, 527
FaqI GGGAC 2 cut(s) 670, 1044
FatI CATG 2 cut(s) 234, 523
FauI CCCGC 2 cut(s) 429, 1069
FbaI TGATCA 1 cut(s) 994
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 2 cut(s) 1065, 1200
FokI GGATG 3 cut(s) 162, 969, 1109
Fsp4HI GCNGC 2 cut(s) 1065, 1200
FspBI CTAG 4 cut(s) 561, 645, 714, 1077
GlaI GCGC 2 cut(s) 847, 922
GluI GCNGC 2 cut(s) 1065, 1200
GsuI CTGGAG 1 cut(s) 246
HaeII RGCGCY 2 cut(s) 849, 924
HaeIII GGCC 2 cut(s) 96, 575
HapII CCGG 3 cut(s) 23, 500, 630
HgaI GACGC 1 cut(s) 588
HhaI GCGC 2 cut(s) 848, 923
Hin1II CATG 2 cut(s) 238, 527
Hin6I GCGC 2 cut(s) 846, 921
HinP1I GCGC 2 cut(s) 846, 921
HincII GTYRAC 2 cut(s) 357, 594
HindII GTYRAC 2 cut(s) 357, 594
HinfI GANTC 8 cut(s) 200, 421, 496, 765, 874, 907, 1042, 1177
HpaII CCGG 3 cut(s) 23, 500, 630
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 6 cut(s) 220, 357, 566, 594, 703, 795
Hpy188III TCNNGA 8 cut(s) 326, 400, 500, 616, 758, 934, 974, 992
Hpy8I GTNNAC 6 cut(s) 220, 357, 566, 594, 703, 795
Hpy99I CGWCG 2 cut(s) 745, 958
HpyAV CCTTC 7 cut(s) 115, 123, 397, 1087, 1100, 1147, 1207
HpyCH4III ACNGT 8 cut(s) 34, 448, 591, 652, 731, 779, 931, 1018
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 5 cut(s) 167, 970, 1014, 1067, 1199
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 245
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 2 cut(s) 238, 527
HspAI GCGC 2 cut(s) 846, 921
Kpn2I TCCGGA 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 994
Kzo9I GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 2 cut(s) 1051, 1211
LweI GCATC 1 cut(s) 979
MaeI CTAG 4 cut(s) 561, 645, 714, 1077
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 3 cut(s) 773, 931, 1022
MalI GATC 9 cut(s) 185, 324, 620, 627, 668, 978, 996, 1038, 1053
MbiI CCGCTC 1 cut(s) 436
MboI GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
MboII GAAGA 7 cut(s) 100, 193, 391, 466, 547, 875, 916
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 317, 725
MluCI AATT 4 cut(s) 256, 301, 1165, 1253
MlyI GAGTC 5 cut(s) 209, 430, 505, 868, 916
MmeI TCCRAC 3 cut(s) 728, 890, 1083
MroI TCCGGA 1 cut(s) 499
MseI TTAA 3 cut(s) 375, 450, 786
MspA1I CMGCKG 1 cut(s) 1064
MspI CCGG 3 cut(s) 23, 500, 630
MspR9I CCNGG 2 cut(s) 264, 630
Mva1269I GAATGC 1 cut(s) 1067
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 759
MwoI GCNNNNNNNGC 2 cut(s) 240, 245
NciI CCSGG 1 cut(s) 630
NdeII GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
NlaIII CATG 2 cut(s) 238, 527
NlaIV GGNNCC 3 cut(s) 39, 134, 627
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 3 cut(s) 773, 931, 1022
NruI TCGCGA 1 cut(s) 759
NspV TTCGAA 1 cut(s) 299
PctI GAATGC 1 cut(s) 1067
PfeI GAWTC 3 cut(s) 765, 1042, 1177
PfoI TCCNGGA 1 cut(s) 262
PkrI GCNGC 2 cut(s) 1066, 1201
PleI GAGTC 5 cut(s) 208, 429, 504, 868, 915
PpsI GAGTC 5 cut(s) 208, 429, 504, 868, 915
Psp1406I AACGTT 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 3 cut(s) 39, 134, 627
PsuI RGATCY 1 cut(s) 625
RruI TCGCGA 1 cut(s) 759
RsaI GTAC 6 cut(s) 27, 68, 197, 634, 702, 1266
RsaNI GTAC 6 cut(s) 26, 67, 196, 633, 701, 1265
SalI GTCGAC 1 cut(s) 355
SaqAI TTAA 3 cut(s) 375, 450, 786
SatI GCNGC 2 cut(s) 1065, 1200
Sau3AI GATC 9 cut(s) 183, 322, 618, 625, 666, 976, 994, 1036, 1051
ScaI AGTACT 1 cut(s) 68
SchI GAGTC 5 cut(s) 209, 430, 505, 868, 916
ScrFI CCNGG 2 cut(s) 264, 630
SduI GDGCHC 2 cut(s) 317, 725
SetI ASST 9 cut(s) 138, 374, 408, 517, 522, 562, 702, 792, 941
SfaNI GCATC 1 cut(s) 979
SfuI TTCGAA 1 cut(s) 299
SpeI ACTAGT 1 cut(s) 1076
Sse9I AATT 4 cut(s) 256, 301, 1165, 1253
SsiI CCGC 7 cut(s) 141, 193, 350, 436, 527, 709, 1062
SspMI CTAG 4 cut(s) 561, 645, 714, 1077
StyD4I CCNGG 2 cut(s) 262, 628
StyI CCWWGG 4 cut(s) 144, 644, 696, 1084
TaaI ACNGT 8 cut(s) 34, 448, 591, 652, 731, 779, 931, 1018
TaiI ACGT 1 cut(s) 374
TasI AATT 4 cut(s) 256, 301, 1165, 1253
TatI WGTACW 1 cut(s) 66
TfiI GAWTC 3 cut(s) 765, 1042, 1177
Tru1I TTAA 3 cut(s) 375, 450, 786
Tru9I TTAA 3 cut(s) 375, 450, 786
TscAI CASTG 4 cut(s) 214, 730, 808, 1021
TseFI GTSAC 3 cut(s) 773, 931, 1022
TseI GCWGC 2 cut(s) 1064, 1199
Tsp45I GTSAC 3 cut(s) 773, 931, 1022
TspDTI ATGAA 6 cut(s) 17, 512, 548, 917, 960, 1166
TspGWI ACGGA 2 cut(s) 295, 638
TspRI CASTG 4 cut(s) 214, 730, 808, 1021
XapI RAATTY 3 cut(s) 256, 301, 1253
XmaJI CCTAGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 356
XspI CTAG 4 cut(s) 561, 645, 714, 1077
ZrmI AGTACT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.