Rroxscaffold_7G00159310

repressing transcription factor binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003679
Physical Location & Seq
Reverse (-)
2531767 .. 2556580
24814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159310.1

Sequence Viewer

Length: 3018 bp
ATGCCGAGAAAAAGAAAAGTACCAATGATTCCGGTCGCCGTGGAACCCCAGATCTGCCCGGAGGCAGTCCGGTGGTGGATGAACGGCGTCGCCAAGTATGAGAAGAAATCTGTGAAGGTGCGTGAGAAGGCCAGAAAACACATGTCGGCCATGGGCTGGAAATTTTGGCACGCAAGGCTTCAGGGAGATAAACCGGTCGAGTTTAGGTACCAATCTCCGGCCGGCAAATTATACTACTCGCTTAAAACGGCTTGCAAAGCCTGCGTTGACCTAGACCTCACTGGTTTCGAAAGTTTCGGTTCAAATAGTTCTGATCCTAATTCTCCTATGGAAGAATCTAAGGAACCAAAATCTTGGATTAATTCCCCCGAAACAAAGGCAAAAAAGAGAGGAAGGAAGAAGAAGATTGATTTATTGATGGCAAAAAAGAGAGGAAGGAAGAGGAAAACTGATTTATTAACGTGTGATCCTAGTTATGATCCTATGGAAGATTGTAAGGAATCAAAATCCTGTACTAATTTCCCCCAATCAAAGCCAAAAAAGAGAGGAAGAAAGAGGAAGATTGATTTAGTAACGCCGGAGGAGGAACAAAAGAGTCTTGAAATTAGTCCTAAGCATAAACCAGGGAAGGTTCTTGCGGAGTTGAAGAGGCTTAGACTTGAAGACGAGCCAAAGTTACAGATTCGAAGTCCGAGAACAATTCTTTCTTCTTTGATAGACAACAAGGTTGTTATGCTACATGCTAAAGTGTACTACCGTGGGAGAAGCGGTGGCAGTCCACTGGCCACGGGCCAAGTTATTCGTGAGGGGATCATGTGTGATTGTTGCGCTAAGGTGTTTGCTCTCACTGCTTTTGAAGCACATGCTGGTAGCACAAATCACAGGCCGGGTGCCAATATCGTTCTCGAGGAAGATGGGAGGACGATTTCTGATTGCCAACGACAGATGAAGAGTTTTAAGGATGAGAAGAAGGTGGTGAAGAGTAGTAATAAGATTGACAAGACTGATGATTTATGCAGTGTGTGTCATAATGAGGGTGAGCTGATTCTGTGTGATAGATGTCCTTCTGCATTCCATACTAGCTGTTTGCGTTTGAGTGAAGTGGCAGATGGCGATTGGTTCTGCCCGGCGTGTTGTTGTGGAATCTGTGGCAGTGGAAAACTTGAACAAGGAACTATTCCTGGTGAGATTAGGAAATGTGATCAGTGTGAGCGTAAGTACCATGTTGGGTGCTTGAGAAACAATGAAGTGGTTTGTGAACAGAATTGGTTTTGTAGTAGTAAATGTCAAAATATTCTGTTTGGGCTGAAAGGGCTTTTGGGACGGCCAATTTTAGTGGGTGACAACAATCTCACTTGGACACTATTGAAGTCTTCCTCCACTAATAGGGCAGACTCCAGAGAAACTCAGAAAAAGCTCAGGTCGGCTCTTAATGTGTTGCATAAGTGTTTTGAGCCTTCTATTGATTCATACACACAAAGGGACCTCGTCGAAGACATTGTTTTAAATAGAGAGTCGAATCTGAGGCGCTTGAATTTCCGAGGGTTTTACACTGTAGTTTTAGAGAGGAATAAGGAAGTGATTTGTGTGGCTACTGTGAGGATCTATGGGGAAGTGGCAGAAATACCTCTTGTGGCCACTATGTTCCGACACCGGAGACTTGGGATGTGTCGGATTTTGATGAATGAGCTGGAAAAACAGCTCAAGAAGTTGGGAGTTGAGAGACTGGTTTTGCCTTCTGCTCCTGCTGCACTGGATACATGGACTAACACTTCGATTGGGTTTTCGAAGATGCCTGAAGCTGAAAAACAACAGTTTATTAATTCTTTTTTCCTGAAGCTTGATTCAATGGGGGAGCCGGTTCGGAATCCGAAACCGAAAAGAGGCAAGGTTCTTGCGGATTTGAAGAGAGTTAGAGATGAAAAGGAGAGGAATAGGGATGTCAAGATGAGGTCAAGTGATGATGAGAACCATGATGAGTGCAGTGTGTGTTGCTATGGAGGGGAATTGGTTCTCTGTGATGGATGTCCCTCTGCTTTTCATGCTGGCTGCGTTGGTTTGGGTGAGGTGGGAGATGGTGATTGGTTCTGCCCATCTTGCTGCTGTGGGTTTTGCAACCTAGGAATATTGGAGGATGATGGTGTTGCCTCATGTGACCAATGCAGGCAGAGGTACCACATTGGGTGTGCAAGAAATTCAGGGGAGGCTAAATTGGGTGTGGATGGGAAATTGTTTTGCAGTAGGAAATGTGAAAATGTGTGTTTGGGAATCAACAAGATTTTGGGGAAGGCTATTGTGGTTGGTCCTGATAATCTTACTTGGACTTTGCTCAAGTCCTCCTCAACTTGTGATGTGGACAACTTGACCAGGAATTACAGACAGCACAAATTGGCTGTGGAGGTGATGCATGAGTGTTTTGAGCCGACTCAGGACCCCTACACGAGAAGTGATGTGGTTGATGATGTGATTTTTAATAGGCAGAAGAAGTTTCGAGGGTTTTATACTGCGGTTTTGGAGAGGAATGAGGAAGTGATTAGCGCAGCTACGGTGAGGGTTTACAGTGAGCTGGGGGAAGTGCCCCTAGTGGCGACTAAATTTAAGCACCGGAGACTTGGGATGTGTCGGATTTTGATGAGTGAGCTTGAGAAACAGCTTGTGAAGTTGGGAATTGGGAAGCTGGTTTTGCCTTCTGCTAAAGATGCACTGAAGGCATGGACTAGTACCTCACTTGGGTTTTCGAAGATGACTGCTGACGAAACATTACCATTGTTGAAGTATAGATTCTGGAATTTCCAAGACACTACCATGTGTCACAAGGTATTAAAGAAGCAGACGACTAGTACTGAATCTGATGGGTCTGCTGGTGCCATTTCTACAGTAACGCAAGTAGGGGACATTGATGCAAGTGAAGAGGCTTGCATTATGGATATTGATTATGATTACGATTTCATGTTGGAGGATGACTCTAAATTCTTGTCATGGTACAAATCTGGATCTAAAAAGATTGTGGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004407 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006476 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0008270 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0015630 GO:0016043 GO:0016462 GO:0016569 GO:0016570 GO:0016575 GO:0016581 GO:0016787 GO:0016810 GO:0016811 GO:0016817 GO:0016818 GO:0016887 GO:0017053 GO:0017111 GO:0019213 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0033558 GO:0035601 GO:0036211 GO:0042623 GO:0042826 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070491 GO:0070603 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090545 GO:0090568 GO:0097159 GO:0098732 GO:0140096 GO:0140097 GO:1901363 GO:1901564 GO:1902494 GO:1903506 GO:1904949 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 Agenet Jas PHD

Protein Analysis

1005

Amino Acids

113.31

Weight (kDa)

8.88

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7028 PF22970 12 - 90 6.1e-24 Domain of unknown function (DUF7028)
TDBD PF16135 264 - 316 1.5e-19 Tify domain binding domain
PHD-1st_NSD PF23011 334 - 378 6.8e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 339 - 378 5e-08 PHD-finger
IDM1_C PF23209 484 - 612 4.9e-45 Increased DNA methylation 1, C-terminal domain
PHD PF00628 661 - 700 5.1e-06 PHD-finger
IDM1_C PF23209 806 - 939 7.3e-40 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 207, 2172
AccB1I GGYRCC 4 cut(s) 207, 890, 2172, 2864
AccB7I CCANNNNNTGG 1 cut(s) 156
AciI CCGC 4 cut(s) 638, 768, 1898, 2507
AclWI GGATC 6 cut(s) 308, 461, 473, 818, 1610, 3001
AcoI YGGCCR 5 cut(s) 147, 219, 783, 1325, 1635
AcsI RAATTY 6 cut(s) 161, 1534, 2194, 2594, 2788, 2969
AcuI CTGAAG 4 cut(s) 164, 1818, 1856, 2726
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 9 cut(s) 21, 209, 514, 752, 1220, 2174, 2722, 2842, 2984
AfiI CCNNNNNNNGG 5 cut(s) 156, 217, 1386, 1883, 2730
AflIII ACRYGT 2 cut(s) 141, 461
AgeI ACCGGT 1 cut(s) 193
AhlI ACTAGT 2 cut(s) 2717, 2837
AjnI CCWGG 3 cut(s) 622, 1180, 2366
AjuI GAANNNNNNNTTGG 2 cut(s) 1301, 1333
Alw26I GTCTC 3 cut(s) 1651, 1717, 2602
AlwI GGATC 6 cut(s) 308, 461, 473, 818, 1610, 3001
Ama87I CYCGRG 1 cut(s) 905
AoxI GGCC 8 cut(s) 129, 147, 219, 783, 790, 884, 1325, 1635
ApeKI GCWGC 4 cut(s) 1748, 2049, 2100, 2540
ApoI RAATTY 6 cut(s) 161, 1534, 2194, 2594, 2788, 2969
ArsI GACNNNNNNTTYG 2 cut(s) 1756, 1788
AseI ATTAAT 2 cut(s) 360, 1821
AsiGI ACCGGT 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 2010
Asp718I GGTACC 2 cut(s) 207, 2172
AspA2I CCTAGG 1 cut(s) 2119
AspLEI GCGC 3 cut(s) 830, 1530, 2540
AspS9I GGNCC 4 cut(s) 790, 1483, 2303, 2431
AsuC2I CCSGG 3 cut(s) 59, 888, 1127
AsuHPI GGTGA 8 cut(s) 988, 1049, 1196, 1352, 2075, 2090, 2413, 2560
AsuII TTCGAA 4 cut(s) 288, 685, 1787, 2738
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 3 cut(s) 1483, 2303, 2431
AvrII CCTAGG 1 cut(s) 2119
BaeGI GKGCMC 1 cut(s) 2580
BalI TGGCCA 2 cut(s) 785, 1637
BanI GGYRCC 4 cut(s) 207, 890, 2172, 2864
BauI CACGAG 1 cut(s) 2440
BbsI GAAGAC 3 cut(s) 669, 1365, 1500
BbvI GCAGC 4 cut(s) 1735, 2036, 2087, 2552
BccI CCATC 9 cut(s) 412, 908, 1103, 2015, 2069, 2101, 2132, 2216, 2846
BceAI ACGGC 4 cut(s) 23, 100, 264, 1340
BciT130I CCWGG 3 cut(s) 624, 1182, 2368
BciVI GTATCC 1 cut(s) 1750
BclI TGATCA 1 cut(s) 1201
BcnI CCSGG 3 cut(s) 59, 888, 1127
BcoDI GTCTC 3 cut(s) 1651, 1717, 2602
BcuI ACTAGT 2 cut(s) 2717, 2837
BfaI CTAG 7 cut(s) 272, 471, 1080, 2120, 2582, 2718, 2838
BfmI CTRYAG 2 cut(s) 1554, 2874
BfoI RGCGCY 1 cut(s) 1531
BfuI GTATCC 1 cut(s) 1750
BglII AGATCT 1 cut(s) 51
BisI GCNGC 4 cut(s) 1749, 2050, 2101, 2541
BlnI CCTAGG 1 cut(s) 2119
BlsI GCNGC 4 cut(s) 1750, 2051, 2102, 2542
BmcAI AGTACT 1 cut(s) 2842
Bme1390I CCNGG 6 cut(s) 59, 624, 888, 1127, 1182, 2368
Bme18I GGWCC 3 cut(s) 1483, 2303, 2431
BmeT110I CYCGRG 1 cut(s) 905
BmgT120I GGNCC 4 cut(s) 790, 1483, 2303, 2431
BmiI GGNNCC 9 cut(s) 45, 209, 345, 892, 1484, 1857, 2174, 2433, 2866
BmrFI CCNGG 6 cut(s) 59, 624, 888, 1127, 1182, 2368
BmsI GCATC 4 cut(s) 1782, 2394, 2689, 2890
BpiI GAAGAC 3 cut(s) 669, 1365, 1500
BplI GAGNNNNNCTC 2 cut(s) 2948, 2980
BpmI CTGGAG 1 cut(s) 1381
Bpu10I CCTNAGC 3 cut(s) 612, 831, 1418
Bpu14I TTCGAA 4 cut(s) 288, 685, 1787, 2738
BpuEI CTTGAG 4 cut(s) 1255, 1688, 2315, 2663
BpuMI CCSGG 3 cut(s) 59, 888, 1127
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 7 cut(s) 39, 150, 623, 757, 786, 1540, 2119
BsaWI WCCGGW 5 cut(s) 31, 69, 193, 1653, 2604
BsaXI ACNNNNNCTCC 2 cut(s) 754, 784
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 217, 1386, 1883, 2730
Bse118I RCCGGY 3 cut(s) 193, 221, 1858
Bse1I ACTGG 4 cut(s) 286, 786, 1731, 1758
BseBI CCWGG 3 cut(s) 624, 1182, 2368
BseDI CCNNGG 7 cut(s) 39, 150, 623, 757, 786, 1540, 2119
BseGI GGATG 9 cut(s) 84, 967, 1671, 1945, 2030, 2140, 2227, 2622, 2965
BseLI CCNNNNNNNGG 5 cut(s) 156, 217, 1386, 1883, 2730
BseMII CTCAG 4 cut(s) 1421, 1432, 1514, 2441
BseNI ACTGG 4 cut(s) 286, 786, 1731, 1758
BseRI GAGGAG 2 cut(s) 596, 2329
BseSI GKGCMC 1 cut(s) 2580
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 4 cut(s) 1735, 2036, 2087, 2552
BseYI CCCAGC 1 cut(s) 2566
BsgI GTGCAG 2 cut(s) 1734, 2002
Bsh1285I CGRYCG 3 cut(s) 36, 198, 222
BshFI GGCC 8 cut(s) 131, 149, 221, 785, 792, 886, 1327, 1637
BshNI GGYRCC 4 cut(s) 207, 890, 2172, 2864
BshTI ACCGGT 1 cut(s) 193
BsiEI CGRYCG 3 cut(s) 36, 198, 222
BsiHKCI CYCGRG 1 cut(s) 905
BslFI GGGAC 4 cut(s) 1335, 1496, 2013, 2906
BslI CCNNNNNNNGG 5 cut(s) 156, 217, 1386, 1883, 2730
BsmAI GTCTC 3 cut(s) 1651, 1717, 2602
BsmFI GGGAC 4 cut(s) 1335, 1496, 2013, 2906
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 8 cut(s) 131, 149, 221, 785, 792, 886, 1327, 1637
BsoBI CYCGRG 1 cut(s) 905
Bsp119I TTCGAA 4 cut(s) 288, 685, 1787, 2738
Bsp1286I GDGCHC 1 cut(s) 2580
Bsp143I GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 4 cut(s) 638, 768, 1898, 2507
BspANI GGCC 8 cut(s) 131, 149, 221, 785, 792, 886, 1327, 1637
BspCNI CTCAG 4 cut(s) 1420, 1431, 1515, 2440
BspLI GGNNCC 9 cut(s) 45, 209, 345, 892, 1484, 1857, 2174, 2433, 2866
BspPI GGATC 6 cut(s) 308, 461, 473, 818, 1610, 3001
BspT104I TTCGAA 4 cut(s) 288, 685, 1787, 2738
BspT107I GGYRCC 4 cut(s) 207, 890, 2172, 2864
BsrFI RCCGGY 3 cut(s) 193, 221, 1858
BsrI ACTGG 4 cut(s) 286, 786, 1731, 1758
BssAI RCCGGY 3 cut(s) 193, 221, 1858
BssECI CCNNGG 7 cut(s) 39, 150, 623, 757, 786, 1540, 2119
BssMI GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 2440
BssT1I CCWWGG 2 cut(s) 150, 2119
Bst2BI CACGAG 1 cut(s) 2440
Bst2UI CCWGG 3 cut(s) 624, 1182, 2368
Bst4CI ACNGT 7 cut(s) 758, 1555, 1597, 1815, 2548, 2561, 2878
Bst6I CTCTTC 6 cut(s) 434, 641, 944, 974, 1901, 2904
BstACI GRCGYC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 261
BstBI TTCGAA 4 cut(s) 288, 685, 1787, 2738
BstC8I GCNNGC 7 cut(s) 171, 223, 253, 262, 2047, 2165, 2917
BstDEI CTNAG 8 cut(s) 339, 612, 653, 831, 1407, 1418, 1523, 2427
BstDSI CCRYGG 4 cut(s) 39, 150, 757, 786
BstF5I GGATG 9 cut(s) 84, 967, 1671, 1945, 2030, 2140, 2227, 2622, 2965
BstH2I RGCGCY 1 cut(s) 1531
BstHHI GCGC 3 cut(s) 830, 1530, 2540
BstKTI GATC 8 cut(s) 54, 316, 469, 481, 813, 1204, 1605, 2996
BstMAI GTCTC 3 cut(s) 1651, 1717, 2602
BstMBI GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
BstMCI CGRYCG 3 cut(s) 36, 198, 222
BstNI CCWGG 3 cut(s) 624, 1182, 2368
BstNSI RCATGY 3 cut(s) 145, 743, 866
BstSCI CCNGG 6 cut(s) 57, 622, 886, 1125, 1180, 2366
BstSFI CTRYAG 2 cut(s) 1554, 2874
BstSLI GKGCMC 1 cut(s) 2580
BstV1I GCAGC 4 cut(s) 1735, 2036, 2087, 2552
BstV2I GAAGAC 3 cut(s) 669, 1365, 1500
BstX2I RGATCY 3 cut(s) 51, 1602, 2993
BstXI CCANNNNNNTGG 1 cut(s) 354
BstYI RGATCY 3 cut(s) 51, 1602, 2993
BstZI CGGCCG 1 cut(s) 219
BsuI GTATCC 1 cut(s) 1750
BsuRI GGCC 8 cut(s) 131, 149, 221, 785, 792, 886, 1327, 1637
BtgI CCRYGG 4 cut(s) 39, 150, 757, 786
BtsCI GGATG 9 cut(s) 84, 967, 1671, 1945, 2030, 2140, 2227, 2622, 2965
BtsI GCAGTG 4 cut(s) 846, 1024, 1159, 1990
Cac8I GCNNGC 7 cut(s) 171, 223, 253, 262, 2047, 2165, 2917
CfoI GCGC 3 cut(s) 830, 1530, 2540
Cfr10I RCCGGY 3 cut(s) 193, 221, 1858
Cfr13I GGNCC 4 cut(s) 790, 1483, 2303, 2431
CseI GACGC 1 cut(s) 76
Csp6I GTAC 9 cut(s) 20, 208, 513, 751, 1219, 2173, 2721, 2841, 2983
CspAI ACCGGT 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 1317, 1352
CviQI GTAC 9 cut(s) 20, 208, 513, 751, 1219, 2173, 2721, 2841, 2983
DdeI CTNAG 8 cut(s) 339, 612, 653, 831, 1407, 1418, 1523, 2427
DpnI GATC 8 cut(s) 53, 315, 468, 480, 812, 1203, 1604, 2995
DpnII GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
DraI TTTAAA 1 cut(s) 1506
EaeI YGGCCR 5 cut(s) 147, 219, 783, 1325, 1635
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 6 cut(s) 434, 641, 944, 974, 1901, 2904
EarI CTCTTC 6 cut(s) 434, 641, 944, 974, 1901, 2904
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 150, 2119
Eco47I GGWCC 3 cut(s) 1483, 2303, 2431
Eco52I CGGCCG 1 cut(s) 219
Eco57I CTGAAG 4 cut(s) 164, 1818, 1856, 2726
Eco88I CYCGRG 1 cut(s) 905
EcoO109I RGGNCCY 2 cut(s) 1483, 2431
EcoRII CCWGG 3 cut(s) 622, 1180, 2366
EcoT14I CCWWGG 2 cut(s) 150, 2119
EcoT22I ATGCAT 1 cut(s) 2409
ErhI CCWWGG 2 cut(s) 150, 2119
FaqI GGGAC 4 cut(s) 1335, 1496, 2013, 2906
FbaI TGATCA 1 cut(s) 1201
Fnu4HI GCNGC 4 cut(s) 1749, 2050, 2101, 2541
FokI GGATG 9 cut(s) 91, 974, 1678, 1952, 2037, 2147, 2234, 2629, 2972
Fsp4HI GCNGC 4 cut(s) 1749, 2050, 2101, 2541
FspBI CTAG 7 cut(s) 272, 471, 1080, 2120, 2582, 2718, 2838
GlaI GCGC 3 cut(s) 829, 1529, 2539
GluI GCNGC 4 cut(s) 1749, 2050, 2101, 2541
GsaI CCCAGC 1 cut(s) 2570
GsuI CTGGAG 1 cut(s) 1381
HaeII RGCGCY 1 cut(s) 1531
HaeIII GGCC 8 cut(s) 131, 149, 221, 785, 792, 886, 1327, 1637
HgaI GACGC 1 cut(s) 76
HhaI GCGC 3 cut(s) 830, 1530, 2540
Hin1I GRCGYC 1 cut(s) 87
Hin6I GCGC 3 cut(s) 828, 1528, 2538
HinP1I GCGC 3 cut(s) 828, 1528, 2538
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 1838
HphI GGTGA 8 cut(s) 988, 1049, 1196, 1352, 2075, 2090, 2413, 2560
Hpy166II GTNNAC 6 cut(s) 268, 751, 779, 1259, 2356, 2557
Hpy8I GTNNAC 6 cut(s) 268, 751, 779, 1259, 2356, 2557
Hpy99I CGWCG 2 cut(s) 92, 1493
HpyCH4III ACNGT 7 cut(s) 758, 1555, 1597, 1815, 2548, 2561, 2878
HpyCH4IV ACGT 1 cut(s) 461
HpyF3I CTNAG 8 cut(s) 339, 612, 653, 831, 1407, 1418, 1523, 2427
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 1 cut(s) 87
HspAI GCGC 3 cut(s) 828, 1528, 2538
KpnI GGTACC 2 cut(s) 211, 2176
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 1201
Kzo9I GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
LmnI GCTCC 2 cut(s) 1747, 1855
Lsp1109I GCAGC 4 cut(s) 1735, 2036, 2087, 2552
LweI GCATC 4 cut(s) 1782, 2394, 2689, 2890
MaeI CTAG 7 cut(s) 272, 471, 1080, 2120, 2582, 2718, 2838
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 6 cut(s) 571, 675, 1340, 2153, 2810, 2878
MalI GATC 8 cut(s) 53, 315, 468, 480, 812, 1203, 1604, 2995
MboI GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
MflI RGATCY 3 cut(s) 51, 1602, 2993
MhlI GDGCHC 1 cut(s) 2580
MlsI TGGCCA 2 cut(s) 785, 1637
MluNI TGGCCA 2 cut(s) 785, 1637
MlyI GAGTC 5 cut(s) 604, 1388, 1523, 2419, 2957
MmeI TCCRAC 4 cut(s) 1652, 1672, 2603, 2934
Mox20I TGGCCA 2 cut(s) 785, 1637
Mph1103I ATGCAT 1 cut(s) 2409
MroNI GCCGGC 1 cut(s) 221
MroXI GAANNNNTTC 1 cut(s) 2010
MscI TGGCCA 2 cut(s) 785, 1637
MslI CAYNNNNRTG 1 cut(s) 2804
Msp20I TGGCCA 2 cut(s) 785, 1637
MspR9I CCNGG 6 cut(s) 59, 624, 888, 1127, 1182, 2368
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 3 cut(s) 624, 1182, 2368
NaeI GCCGGC 1 cut(s) 223
NciI CCSGG 3 cut(s) 59, 888, 1127
NcoI CCATGG 1 cut(s) 150
NdeII GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
NgoMIV GCCGGC 1 cut(s) 221
NlaIV GGNNCC 9 cut(s) 45, 209, 345, 892, 1484, 1857, 2174, 2433, 2866
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 1340, 2153, 2810
NsiI ATGCAT 1 cut(s) 2409
NspI RCATGY 3 cut(s) 145, 743, 866
NspV TTCGAA 4 cut(s) 288, 685, 1787, 2738
PaeR7I CTCGAG 1 cut(s) 905
PciI ACATGT 1 cut(s) 141
PctI GAATGC 1 cut(s) 1070
PdiI GCCGGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 2010
PflFI GACNNNGTC 1 cut(s) 1487
PflMI CCANNNNNTGG 1 cut(s) 156
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 4 cut(s) 1750, 2051, 2102, 2542
PleI GAGTC 5 cut(s) 603, 1388, 1522, 2419, 2957
PpsI GAGTC 5 cut(s) 603, 1388, 1522, 2419, 2957
PpuMI RGGWCCY 2 cut(s) 1483, 2431
PscI ACATGT 1 cut(s) 141
PshBI ATTAAT 2 cut(s) 360, 1821
Psp5II RGGWCCY 2 cut(s) 1483, 2431
Psp6I CCWGG 3 cut(s) 622, 1180, 2366
PspFI CCCAGC 1 cut(s) 2566
PspGI CCWGG 3 cut(s) 622, 1180, 2366
PspN4I GGNNCC 9 cut(s) 45, 209, 345, 892, 1484, 1857, 2174, 2433, 2866
PspPI GGNCC 4 cut(s) 790, 1483, 2303, 2431
PspPPI RGGWCCY 2 cut(s) 1483, 2431
PsuI RGATCY 3 cut(s) 51, 1602, 2993
PsyI GACNNNGTC 1 cut(s) 1487
RsaI GTAC 9 cut(s) 21, 209, 514, 752, 1220, 2174, 2722, 2842, 2984
RsaNI GTAC 9 cut(s) 20, 208, 513, 751, 1219, 2173, 2721, 2841, 2983
RseI CAYNNNNRTG 1 cut(s) 2804
SatI GCNGC 4 cut(s) 1749, 2050, 2101, 2541
Sau3AI GATC 8 cut(s) 51, 313, 466, 478, 810, 1201, 1602, 2993
Sau96I GGNCC 4 cut(s) 790, 1483, 2303, 2431
ScaI AGTACT 1 cut(s) 2842
SchI GAGTC 5 cut(s) 604, 1388, 1523, 2419, 2957
ScrFI CCNGG 6 cut(s) 59, 624, 888, 1127, 1182, 2368
SduI GDGCHC 1 cut(s) 2580
SfaNI GCATC 4 cut(s) 1782, 2394, 2689, 2890
SfcI CTRYAG 2 cut(s) 1554, 2874
Sfr274I CTCGAG 1 cut(s) 905
SfuI TTCGAA 4 cut(s) 288, 685, 1787, 2738
SinI GGWCC 3 cut(s) 1483, 2303, 2431
SlaI CTCGAG 1 cut(s) 905
SmiMI CAYNNNNRTG 1 cut(s) 2804
SmlI CTYRAG 5 cut(s) 905, 1234, 1703, 2330, 2642
SmoI CTYRAG 5 cut(s) 905, 1234, 1703, 2330, 2642
SpeI ACTAGT 2 cut(s) 2717, 2837
SsiI CCGC 4 cut(s) 638, 768, 1898, 2507
SspI AATATT 2 cut(s) 1294, 2127
SspMI CTAG 7 cut(s) 272, 471, 1080, 2120, 2582, 2718, 2838
StyD4I CCNGG 6 cut(s) 57, 622, 886, 1125, 1180, 2366
StyI CCWWGG 2 cut(s) 150, 2119
TaaI ACNGT 7 cut(s) 758, 1555, 1597, 1815, 2548, 2561, 2878
TaiI ACGT 1 cut(s) 464
TatI WGTACW 3 cut(s) 512, 750, 2840
TseFI GTSAC 3 cut(s) 1340, 2153, 2810
TseI GCWGC 4 cut(s) 1748, 2049, 2100, 2540
Tsp45I GTSAC 3 cut(s) 1340, 2153, 2810
TspDTI ATGAA 8 cut(s) 95, 962, 1260, 1458, 1697, 1935, 2030, 2938
Tth111I GACNNNGTC 1 cut(s) 1487
Van91I CCANNNNNTGG 1 cut(s) 156
VpaK11BI GGWCC 3 cut(s) 1483, 2303, 2431
VspI ATTAAT 2 cut(s) 360, 1821
XapI RAATTY 6 cut(s) 161, 1534, 2194, 2594, 2788, 2969
XceI RCATGY 3 cut(s) 145, 743, 866
XhoI CTCGAG 1 cut(s) 905
XmaJI CCTAGG 1 cut(s) 2119
XmnI GAANNNNTTC 1 cut(s) 2010
XspI CTAG 7 cut(s) 272, 471, 1080, 2120, 2582, 2718, 2838
ZrmI AGTACT 1 cut(s) 2842
Zsp2I ATGCAT 1 cut(s) 2409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.