Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002673

Physical Location & Seq

Genomic Coordinates Forward (+)

12477467 .. 12480669

Gene Structure 3203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1132300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGGAAAAGCTGTCACCTTCTTCTTCGTCTGTCGACCACAAAGCTTGGACAGAGGCCCAAGATTTGCTGAAGCAAAAATTGATTGCAGAAGATGATTTCTCATGGAGATTAGATTCAAAGTCAAACTCAAGGTCAATGGAGAGAGAGGAGGAGAAAGAGGAGGAGAGAGAGGAGCTGTTGAAGTATGTGGGAGGGGTTGATATAAGCTATTCAAAAGAAAACCCATCAATGGCATGTGGAATTCTTGTGGTTTTGGAGCTCAAAACTCTCCAAGTTGTGTATCAAGACTTCTGTGTTGTCATTCCCAATGTGCCTTATGTTCCTGGCTTCCTTGCCTTCAGAGAGGCCCCAGTGTTGCTTGAGCTTTTGGAGAAAATGAAAAACAACAATAGTCCTTTCTACCCACAGCTTCTAATGGTTGATGGAAACGGAATACTTCATCCTCGAGGTTTTGGCTTGGCTTGTCATCTTGGCATTCTGGCAAATCTACCTACTATTGGGATCGGAAAAAATCTTCATCATGTGGATGGTCTAACACTATCCGGAGTGAAGCAGCTTCTTGAAGCCCAAGAAGACAGTGCTGAAGGTTTTATTACTTTGAAAGGATGTTCAGGCCGTACATGGGGAGCGGCAGCAAGATCTACAGGTGGCTCATTGAAACCTATATTTATTTCAACTGGTCACCGTATATCACTTGATACTGCCACCAGAATTGTGAAAATGACTTGCAAATTTCGTGTCCCTGAGCCTATCCGGCAGGCTGATATAAGATCTAGAGACTATCTTCGGAAGCATCAAACGAGAACATCCAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004521 GO:0004536 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0016888 GO:0016891 GO:0016893 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046872 GO:0050896 GO:0051716 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Endonuclease_5

Protein Analysis

273

Amino Acids

30.37

Weight (kDa)

7.05

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Endonuclease_5	PF04493	50 - 255	7.5e-63	Endonuclease V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002673)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31150 AT4G31150 AT4G31150 AT4G31150
fragaria_vesca	FvH4_2g30570 FvH4_2g30570 FvH4_2g30570 FvH4_2g30570
malus_domestica	MD08G1132300.v1.1
prunus_persica	Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1 Prupe.1G463600_v2.0.a1
pyrus_communis	pycom08g11070
rosa_chinensis	RchiOBHm_Chr6g0312441 RchiOBHm_Chr6g0312501
rosa_laevigata	RLG00000010323 RLG00000010328
rosa_multiflora	Rmu_sc0008363.1_g000018
rosa_roxburghii	Rroxscaffold_1G00063820 Rroxscaffold_2G00098010 Rroxscaffold_7G00156790
rosa_rugosa	Rorug06G0395800 Rorug06G0396000
rosa_samantha	Rh4DG302300 Rh5CG565400 Rh6AG513200 Rh6BG524000 Rh6CG529500 Rh6DG514200 Rh6DG515300
rosa_wichuraiana	Rw6G044610 Rw6G044630 Rw6G044680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	444
AccBSI	CCGCTC	1 cut(s)	629
AccI	GTMKAC	1 cut(s)	33
AccIII	TCCGGA	1 cut(s)	542
AciI	CCGC	1 cut(s)	629
AclWI	GGATC	1 cut(s)	509
AcsI	RAATTY	2 cut(s)	240, 731
AcuI	CTGAAG	3 cut(s)	89, 322, 603
AfaI	GTAC	1 cut(s)	619
AfiI	CCNNNNNNNGG	3 cut(s)	229, 497, 622
AgsI	TTSAA	7 cut(s)	117, 181, 213, 563, 601, 658, 675
AjnI	CCWGG	1 cut(s)	322
AluBI	AGCT	8 cut(s)	10, 44, 175, 207, 259, 364, 409, 556
AluI	AGCT	8 cut(s)	10, 44, 175, 207, 259, 364, 409, 556
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	1 cut(s)	771
AlwI	GGATC	1 cut(s)	509
Ama87I	CYCGRG	1 cut(s)	444
Aor13HI	TCCGGA	1 cut(s)	542
AoxI	GGCC	3 cut(s)	54, 345, 613
ApeKI	GCWGC	2 cut(s)	553, 632
ApoI	RAATTY	2 cut(s)	240, 731
ArsI	GACNNNNNNTTYG	4 cut(s)	116, 148, 723, 755
AspS9I	GGNCC	2 cut(s)	55, 346
AsuHPI	GGTGA	2 cut(s)	6, 674
AvaI	CYCGRG	1 cut(s)	444
BanII	GRGCYC	1 cut(s)	261
BarI	GAAGNNNNNNTAC	2 cut(s)	272, 304
BbsI	GAAGAC	1 cut(s)	579
Bbv12I	GWGCWC	1 cut(s)	261
BbvI	GCAGC	2 cut(s)	565, 644
BccI	CCATC	3 cut(s)	232, 416, 521
BceAI	ACGGC	1 cut(s)	600
BciT130I	CCWGG	1 cut(s)	324
BcoDI	GTCTC	1 cut(s)	771
BfaI	CTAG	1 cut(s)	774
BfmI	CTRYAG	1 cut(s)	642
BglI	GCCNNNNNGGC	1 cut(s)	754
BglII	AGATCT	2 cut(s)	638, 770
BisI	GCNGC	3 cut(s)	554, 630, 633
BlsI	GCNGC	3 cut(s)	555, 631, 634
Bme1390I	CCNGG	1 cut(s)	324
BmeT110I	CYCGRG	1 cut(s)	444
BmgT120I	GGNCC	2 cut(s)	55, 346
BmiI	GGNNCC	1 cut(s)	348
BmrFI	CCNGG	1 cut(s)	324
BmrI	ACTGGG	1 cut(s)	344
BmsI	GCATC	1 cut(s)	802
BmuI	ACTGGG	1 cut(s)	344
BpiI	GAAGAC	1 cut(s)	579
Bpu10I	CCTNAGC	1 cut(s)	744
BpuEI	CTTGAG	2 cut(s)	112, 380
BsaWI	WCCGGW	1 cut(s)	542
Bsc4I	CCNNNNNNNGG	3 cut(s)	229, 497, 622
Bse1I	ACTGG	2 cut(s)	350, 682
BseAI	TCCGGA	1 cut(s)	542
BseBI	CCWGG	1 cut(s)	324
BseGI	GGATG	4 cut(s)	439, 532, 611, 806
BseLI	CCNNNNNNNGG	3 cut(s)	229, 497, 622
BseMII	CTCAG	1 cut(s)	735
BseNI	ACTGG	2 cut(s)	350, 682
BseRI	GAGGAG	5 cut(s)	161, 164, 173, 176, 185
BseXI	GCAGC	2 cut(s)	565, 644
BshFI	GGCC	3 cut(s)	56, 347, 615
BsiHKAI	GWGCWC	1 cut(s)	261
BsiHKCI	CYCGRG	1 cut(s)	444
BsiSI	CCGG	2 cut(s)	543, 754
BslFI	GGGAC	1 cut(s)	725
BslI	CCNNNNNNNGG	3 cut(s)	229, 497, 622
BsmAI	GTCTC	1 cut(s)	771
BsmFI	GGGAC	1 cut(s)	725
BsmI	GAATGC	1 cut(s)	474
BsnI	GGCC	3 cut(s)	56, 347, 615
BsoBI	CYCGRG	1 cut(s)	444
Bsp1286I	GDGCHC	1 cut(s)	261
Bsp13I	TCCGGA	1 cut(s)	542
Bsp143I	GATC	3 cut(s)	501, 638, 770
BspACI	CCGC	1 cut(s)	629
BspANI	GGCC	3 cut(s)	56, 347, 615
BspCNI	CTCAG	1 cut(s)	736
BspEI	TCCGGA	1 cut(s)	542
BspLI	GGNNCC	1 cut(s)	348
BspPI	GGATC	1 cut(s)	509
BsrBI	CCGCTC	1 cut(s)	629
BsrI	ACTGG	2 cut(s)	350, 682
BssMI	GATC	3 cut(s)	501, 638, 770
Bst2UI	CCWGG	1 cut(s)	324
Bst4CI	ACNGT	2 cut(s)	578, 686
BstC8I	GCNNGC	1 cut(s)	759
BstDEI	CTNAG	1 cut(s)	744
BstEII	GGTNACC	1 cut(s)	680
BstF5I	GGATG	4 cut(s)	439, 532, 611, 806
BstKTI	GATC	3 cut(s)	504, 641, 773
BstMAI	GTCTC	1 cut(s)	771
BstMBI	GATC	3 cut(s)	501, 638, 770
BstMWI	GCNNNNNNNGC	1 cut(s)	754
BstNI	CCWGG	1 cut(s)	324
BstNSI	RCATGY	1 cut(s)	237
BstPI	GGTNACC	1 cut(s)	680
BstSCI	CCNGG	1 cut(s)	322
BstSFI	CTRYAG	1 cut(s)	642
BstV1I	GCAGC	2 cut(s)	565, 644
BstV2I	GAAGAC	1 cut(s)	579
BstX2I	RGATCY	2 cut(s)	638, 770
BstYI	RGATCY	2 cut(s)	638, 770
BsuRI	GGCC	3 cut(s)	56, 347, 615
BtsCI	GGATG	4 cut(s)	439, 532, 611, 806
BtsIMutI	CAGTG	2 cut(s)	357, 583
Cac8I	GCNNGC	1 cut(s)	759
Cfr13I	GGNCC	2 cut(s)	55, 346
Csp6I	GTAC	1 cut(s)	618
CspCI	CAANNNNNGTGG	4 cut(s)	26, 61, 694, 729
CviAII	CATG	4 cut(s)	102, 234, 521, 621
CviQI	GTAC	1 cut(s)	618
DdeI	CTNAG	1 cut(s)	744
DpnI	GATC	3 cut(s)	503, 640, 772
DpnII	GATC	3 cut(s)	501, 638, 770
Ecl136II	GAGCTC	1 cut(s)	259
Eco24I	GRGCYC	1 cut(s)	261
Eco53kI	GAGCTC	1 cut(s)	259
Eco57I	CTGAAG	3 cut(s)	89, 322, 603
Eco88I	CYCGRG	1 cut(s)	444
Eco91I	GGTNACC	1 cut(s)	680
EcoICRI	GAGCTC	1 cut(s)	259
EcoO109I	RGGNCCY	1 cut(s)	346
EcoO65I	GGTNACC	1 cut(s)	680
EcoRI	GAATTC	1 cut(s)	240
EcoRII	CCWGG	1 cut(s)	322
EcoT38I	GRGCYC	1 cut(s)	261
FaeI	CATG	4 cut(s)	105, 237, 524, 624
FaqI	GGGAC	1 cut(s)	725
FatI	CATG	4 cut(s)	101, 233, 520, 620
FblI	GTMKAC	1 cut(s)	33
Fnu4HI	GCNGC	3 cut(s)	554, 630, 633
FokI	GGATG	4 cut(s)	426, 539, 618, 793
FriOI	GRGCYC	1 cut(s)	261
Fsp4HI	GCNGC	3 cut(s)	554, 630, 633
FspBI	CTAG	1 cut(s)	774
GluI	GCNGC	3 cut(s)	554, 630, 633
HaeIII	GGCC	3 cut(s)	56, 347, 615
HapII	CCGG	2 cut(s)	543, 754
Hin1II	CATG	4 cut(s)	105, 237, 524, 624
HincII	GTYRAC	1 cut(s)	34
HindII	GTYRAC	1 cut(s)	34
HindIII	AAGCTT	1 cut(s)	42
HinfI	GANTC	1 cut(s)	113
HpaII	CCGG	2 cut(s)	543, 754
HphI	GGTGA	2 cut(s)	6, 674
Hpy166II	GTNNAC	1 cut(s)	34
Hpy188I	TCNGA	3 cut(s)	341, 506, 789
Hpy188III	TCNNGA	4 cut(s)	284, 543, 560, 774
Hpy8I	GTNNAC	1 cut(s)	34
HpyAV	CCTTC	3 cut(s)	27, 346, 578
HpyCH4III	ACNGT	2 cut(s)	578, 686
HpyCH4V	TGCA	2 cut(s)	86, 729
HpyF10VI	GCNNNNNNNGC	1 cut(s)	754
HpyF3I	CTNAG	1 cut(s)	744
Hsp92II	CATG	4 cut(s)	105, 237, 524, 624
Kpn2I	TCCGGA	1 cut(s)	542
Kzo9I	GATC	3 cut(s)	501, 638, 770
LmnI	GCTCC	3 cut(s)	172, 256, 626
Lsp1109I	GCAGC	2 cut(s)	565, 644
LweI	GCATC	1 cut(s)	802
MaeI	CTAG	1 cut(s)	774
MaeIII	GTNAC	2 cut(s)	12, 680
MalI	GATC	3 cut(s)	503, 640, 772
MbiI	CCGCTC	1 cut(s)	629
MboI	GATC	3 cut(s)	501, 638, 770
MboII	GAAGA	6 cut(s)	12, 15, 101, 506, 584, 776
MflI	RGATCY	2 cut(s)	638, 770
MhlI	GDGCHC	1 cut(s)	261
MluCI	AATT	4 cut(s)	77, 240, 711, 731
MroI	TCCGGA	1 cut(s)	542
MslI	CAYNNNNRTG	1 cut(s)	525
MspI	CCGG	2 cut(s)	543, 754
MspR9I	CCNGG	1 cut(s)	324
Mva1269I	GAATGC	1 cut(s)	474
MvaI	CCWGG	1 cut(s)	324
MwoI	GCNNNNNNNGC	1 cut(s)	754
NdeII	GATC	3 cut(s)	501, 638, 770
NlaIII	CATG	4 cut(s)	105, 237, 524, 624
NlaIV	GGNNCC	1 cut(s)	348
NmuCI	GTSAC	2 cut(s)	12, 680
NspI	RCATGY	1 cut(s)	237
PaeR7I	CTCGAG	1 cut(s)	444
PctI	GAATGC	1 cut(s)	474
PfeI	GAWTC	1 cut(s)	113
PkrI	GCNGC	3 cut(s)	555, 631, 634
Psp124BI	GAGCTC	1 cut(s)	261
Psp6I	CCWGG	1 cut(s)	322
PspEI	GGTNACC	1 cut(s)	680
PspGI	CCWGG	1 cut(s)	322
PspN4I	GGNNCC	1 cut(s)	348
PspPI	GGNCC	2 cut(s)	55, 346
PspXI	VCTCGAGB	1 cut(s)	444
PsuI	RGATCY	2 cut(s)	638, 770
RsaI	GTAC	1 cut(s)	619
RsaNI	GTAC	1 cut(s)	618
RseI	CAYNNNNRTG	1 cut(s)	525
SacI	GAGCTC	1 cut(s)	261
SalI	GTCGAC	1 cut(s)	32
SatI	GCNGC	3 cut(s)	554, 630, 633
Sau3AI	GATC	3 cut(s)	501, 638, 770
Sau96I	GGNCC	2 cut(s)	55, 346
ScrFI	CCNGG	1 cut(s)	324
SduI	GDGCHC	1 cut(s)	261
SfaNI	GCATC	1 cut(s)	802
SfcI	CTRYAG	1 cut(s)	642
Sfr274I	CTCGAG	1 cut(s)	444
SlaI	CTCGAG	1 cut(s)	444
SmiMI	CAYNNNNRTG	1 cut(s)	525
SmlI	CTYRAG	3 cut(s)	127, 359, 444
SmoI	CTYRAG	3 cut(s)	127, 359, 444
Sse9I	AATT	4 cut(s)	77, 240, 711, 731
SsiI	CCGC	1 cut(s)	629
SspMI	CTAG	1 cut(s)	774
SstI	GAGCTC	1 cut(s)	261
StyD4I	CCNGG	1 cut(s)	322
TaaI	ACNGT	2 cut(s)	578, 686
TaqI	TCGA	2 cut(s)	33, 445
TasI	AATT	4 cut(s)	77, 240, 711, 731
TauI	GCSGC	1 cut(s)	632
TfiI	GAWTC	1 cut(s)	113
TscAI	CASTG	2 cut(s)	357, 583
TseFI	GTSAC	2 cut(s)	12, 680
TseI	GCWGC	2 cut(s)	553, 632
Tsp45I	GTSAC	2 cut(s)	12, 680
TspDTI	ATGAA	3 cut(s)	392, 428, 506
TspGWI	ACGGA	1 cut(s)	444
TspRI	CASTG	2 cut(s)	357, 583
XapI	RAATTY	2 cut(s)	240, 731
XbaI	TCTAGA	1 cut(s)	773
XceI	RCATGY	1 cut(s)	237
XhoI	CTCGAG	1 cut(s)	444
XmiI	GTMKAC	1 cut(s)	33
XspI	CTAG	1 cut(s)	774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.