MD08G1150300.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004715
Physical Location & Seq
Reverse (-)
15231553 .. 15236125
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1150300.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGAATGGGACCTTCAGCGAGAATGCTATGACGGAGACTGCAGCATCAAGTGGGAAAATTGGAAATGCTGATAAGGTGGATGACGGTGGGACTAATAGCTCGTCTGTTTGGGAAGCTATAGAGGCCAATGTCAACCCTGAAAGTAATGGTTTGACTGTTGCTTTGACTATTGCTAAGGAAGATGAAGTGAAGGGTTCTACTCGTTCAAAGCCCGCCAAAGTGCATAAAGGTCCAGGTAAGAGCAAGAATGAGAAGGCCCCTGGTCCCAAGAGCGTCTCCCCAACTTTGATGAAGAAAAGCAAAGATGGAAATGGCACTGAGGCAAAAGCTTCTGTTTCAAATGGTTCAGCTGCTCCAAATTCATGCCCGAAACAGCCTAACAAGAATAGATTATTTAATGGGAGACAGGTTCAGTCATCCAATGTAAACCAACCTGAAAAACTTGATGCAGAATTGTCTGAAGGCAGCGTGGAGAAGGCGAAGCTGAAGCCTTTGAAAAAAGGCTCCCAGAATCAGGCTGAAGGAGAATCACAATCCTCTTTTCCAACAGAAGGAGATAAACAGCCTAGGGTTGGGACACTTCCGAAATATGGTTTCAGTTTTAGGTGCGATGAACGAGCTGAGAAAAGGAGAGAGTTCTATACTAAACTGGAGGAAAAGATACATGCAAAGGAGATGGAAAAGAATAATTTGCAAGCCAAGTCCGAGGTGGTATTCAATCAATGGTGGTCAACCGAAACCTGTGAAACTCTGGAAGCTGAGATTAGGATGCTCAGGAAAAAACTGACTTTTAAAGCAACTCCAATGCCAAGCTTCTATCAGGAGCCTCCACCTCCTAAAGTGGAGCTGAAGAAGATACCAACTACAAGAGCCAAGTCTCCCAAGCTCGGACGTAGGAAGAGCTTACCTCCTGCAGTTTCTGAAGCACACGGTAATACCAATGGTCGATCTAGCAGGCTTAGTCTGGATGAGAAAGTACCTCAGAACACTGCTAAAGGGCCCTCTCCTGTGCATCCAAAGAAGCCACAACGGAAGTCTCTTCCTAGACTCCCGTCTGAGAAGACCACTTTACCAAATGTGGTGAATGAGCCAAAAATGACTTCTAAGGCAGTAAATGAGGAAAAGACCAACTTAATTGCTGCAACGATTGAGGAGAATGCCACCATGCCCACTGCAGAAAGTGAAGCTGGATCTCACACCCAAGAGGAGGCAGCAGTTCCCAAGGCCGAGGTAAGCAAAGAGCAGTCCCATACAGATGATGAGACAGCGGATGAAGACCAACACCAACCCGTCTATGTTCAAGAACCTATAACCTCGGAGCATTGA

Protein Analysis

442

Amino Acids

48.14

Weight (kDa)

8.96

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 199 - 286 3.5e-20 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 213, 1268
AclWI GGATC 1 cut(s) 1198
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 5 cut(s) 480, 506, 540, 869, 942
AfaI GTAC 1 cut(s) 978
AfiI CCNNNNNNNGG 6 cut(s) 514, 551, 572, 590, 887, 1207
AgsI TTSAA 5 cut(s) 207, 339, 496, 718, 1301
AjnI CCWGG 2 cut(s) 232, 259
AjuI GAANNNNNNNTTGG 2 cut(s) 1084, 1116
Alw26I GTCTC 6 cut(s) 29, 280, 397, 882, 1041, 1256
AlwI GGATC 1 cut(s) 1198
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 4 cut(s) 123, 255, 998, 1224
ApaI GGGCCC 1 cut(s) 1002
ApeKI GCWGC 5 cut(s) 41, 350, 465, 1139, 1211
ApoI RAATTY 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspA2I CCTAGG 1 cut(s) 566
AspS9I GGNCC 6 cut(s) 9, 230, 256, 263, 998, 999
AsuHPI GGTGA 1 cut(s) 1093
AvaII GGWCC 3 cut(s) 9, 230, 263
AvrII CCTAGG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 1002
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 2 cut(s) 1067, 1281
BbvI GCAGC 5 cut(s) 53, 337, 477, 1126, 1223
BccI CCATC 2 cut(s) 299, 670
BciT130I CCWGG 2 cut(s) 234, 261
BcoDI GTCTC 6 cut(s) 29, 280, 397, 882, 1041, 1256
BfaI CTAG 3 cut(s) 567, 951, 1044
BfmI CTRYAG 4 cut(s) 39, 117, 912, 1173
BisI GCNGC 5 cut(s) 42, 351, 466, 1140, 1212
BlnI CCTAGG 1 cut(s) 566
BlsI GCNGC 5 cut(s) 43, 352, 467, 1141, 1213
Bme1390I CCNGG 2 cut(s) 234, 261
Bme18I GGWCC 3 cut(s) 9, 230, 263
BmgT120I GGNCC 6 cut(s) 9, 230, 256, 263, 998, 999
BmiI GGNNCC 6 cut(s) 10, 258, 265, 505, 825, 1000
BmrFI CCNGG 2 cut(s) 234, 261
BmsI GCATC 4 cut(s) 53, 436, 759, 1021
BoxI GACNNNNGTC 1 cut(s) 1051
BpiI GAAGAC 2 cut(s) 1067, 1281
BplI GAGNNNNNCTC 2 cut(s) 892, 924
BpmI CTGGAG 1 cut(s) 671
Bpu10I CCTNAGC 2 cut(s) 174, 773
BsaJI CCNNGG 6 cut(s) 259, 566, 705, 1221, 1227, 1314
Bsc4I CCNNNNNNNGG 6 cut(s) 514, 551, 572, 590, 887, 1207
Bse1I ACTGG 1 cut(s) 654
BseBI CCWGG 2 cut(s) 234, 261
BseDI CCNNGG 6 cut(s) 259, 566, 705, 1221, 1227, 1314
BseGI GGATG 6 cut(s) 85, 416, 774, 973, 1012, 1276
BseLI CCNNNNNNNGG 6 cut(s) 514, 551, 572, 590, 887, 1207
BseMII CTCAG 6 cut(s) 309, 612, 750, 787, 995, 1047
BseNI ACTGG 1 cut(s) 654
BseRI GAGGAG 2 cut(s) 1166, 1220
BseSI GKGCMC 1 cut(s) 1002
BseXI GCAGC 5 cut(s) 53, 337, 477, 1126, 1223
BshFI GGCC 4 cut(s) 125, 257, 1000, 1226
BslFI GGGAC 5 cut(s) 22, 103, 249, 589, 1231
BslI CCNNNNNNNGG 6 cut(s) 514, 551, 572, 590, 887, 1207
BsmAI GTCTC 6 cut(s) 29, 280, 397, 882, 1041, 1256
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 5 cut(s) 22, 103, 249, 589, 1231
BsmI GAATGC 2 cut(s) 28, 1162
BsnI GGCC 4 cut(s) 125, 257, 1000, 1226
Bsp120I GGGCCC 1 cut(s) 998
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp143I GATC 2 cut(s) 947, 1190
BspACI CCGC 2 cut(s) 213, 1268
BspANI GGCC 4 cut(s) 125, 257, 1000, 1226
BspCNI CTCAG 6 cut(s) 310, 613, 751, 786, 994, 1048
BspLI GGNNCC 6 cut(s) 10, 258, 265, 505, 825, 1000
BspMAI CTGCAG 3 cut(s) 43, 916, 1177
BspPI GGATC 1 cut(s) 1198
BspQI GCTCTTC 1 cut(s) 893
BsrI ACTGG 1 cut(s) 654
BssECI CCNNGG 6 cut(s) 259, 566, 705, 1221, 1227, 1314
BssMI GATC 2 cut(s) 947, 1190
BssT1I CCWWGG 2 cut(s) 566, 1221
Bst2UI CCWGG 2 cut(s) 234, 261
Bst4CI ACNGT 3 cut(s) 86, 157, 932
Bst6I CTCTTC 2 cut(s) 893, 1044
BstC8I GCNNGC 3 cut(s) 213, 696, 956
BstDEI CTNAG 9 cut(s) 174, 318, 621, 759, 773, 959, 981, 1056, 1104
BstF5I GGATG 6 cut(s) 85, 416, 774, 973, 1012, 1276
BstKTI GATC 2 cut(s) 950, 1193
BstMAI GTCTC 6 cut(s) 29, 280, 397, 882, 1041, 1256
BstMBI GATC 2 cut(s) 947, 1190
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 2 cut(s) 234, 261
BstNSI RCATGY 1 cut(s) 668
BstPAI GACNNNNGTC 1 cut(s) 1051
BstSCI CCNGG 2 cut(s) 232, 259
BstSFI CTRYAG 4 cut(s) 39, 117, 912, 1173
BstSLI GKGCMC 1 cut(s) 1002
BstV1I GCAGC 5 cut(s) 53, 337, 477, 1126, 1223
BstV2I GAAGAC 2 cut(s) 1067, 1281
BstX2I RGATCY 1 cut(s) 1190
BstYI RGATCY 1 cut(s) 1190
BsuRI GGCC 4 cut(s) 125, 257, 1000, 1226
BtgZI GCGATG 1 cut(s) 624
BtsCI GGATG 6 cut(s) 85, 416, 774, 973, 1012, 1276
BtsI GCAGTG 2 cut(s) 987, 1170
BtsIMutI CAGTG 3 cut(s) 315, 987, 1170
Cac8I GCNNGC 3 cut(s) 213, 696, 956
CaiI CAGNNNCTG 1 cut(s) 920
Cfr13I GGNCC 6 cut(s) 9, 230, 256, 263, 998, 999
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 977
CviAII CATG 3 cut(s) 363, 665, 1165
CviQI GTAC 1 cut(s) 977
DdeI CTNAG 9 cut(s) 174, 318, 621, 759, 773, 959, 981, 1056, 1104
DpnI GATC 2 cut(s) 949, 1192
DpnII GATC 2 cut(s) 947, 1190
DraI TTTAAA 1 cut(s) 793
Eam1104I CTCTTC 2 cut(s) 893, 1044
EarI CTCTTC 2 cut(s) 893, 1044
Eco130I CCWWGG 2 cut(s) 566, 1221
Eco24I GRGCYC 1 cut(s) 1002
Eco47I GGWCC 3 cut(s) 9, 230, 263
Eco57I CTGAAG 5 cut(s) 480, 506, 540, 869, 942
EcoO109I RGGNCCY 4 cut(s) 9, 256, 998, 999
EcoRII CCWGG 2 cut(s) 232, 259
EcoT14I CCWWGG 2 cut(s) 566, 1221
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 2 cut(s) 566, 1221
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 3 cut(s) 366, 668, 1168
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FaqI GGGAC 5 cut(s) 22, 103, 249, 589, 1231
FatI CATG 3 cut(s) 362, 664, 1164
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 5 cut(s) 42, 351, 466, 1140, 1212
FokI GGATG 6 cut(s) 92, 403, 781, 980, 999, 1283
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 5 cut(s) 42, 351, 466, 1140, 1212
FspBI CTAG 3 cut(s) 567, 951, 1044
GluI GCNGC 5 cut(s) 42, 351, 466, 1140, 1212
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 4 cut(s) 125, 257, 1000, 1226
HgaI GACGC 1 cut(s) 262
Hin1II CATG 3 cut(s) 366, 668, 1168
HincII GTYRAC 2 cut(s) 133, 732
HindII GTYRAC 2 cut(s) 133, 732
HindIII AAGCTT 2 cut(s) 327, 811
HinfI GANTC 3 cut(s) 511, 527, 1047
HphI GGTGA 1 cut(s) 1093
Hpy166II GTNNAC 3 cut(s) 133, 427, 732
Hpy188I TCNGA 8 cut(s) 460, 585, 706, 890, 922, 984, 1057, 1318
Hpy188III TCNNGA 5 cut(s) 752, 775, 821, 965, 1301
Hpy8I GTNNAC 3 cut(s) 133, 427, 732
HpyAV CCTTC 7 cut(s) 22, 184, 247, 455, 469, 515, 545
HpyCH4III ACNGT 3 cut(s) 86, 157, 932
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 9 cut(s) 41, 223, 449, 668, 694, 914, 1012, 1142, 1175
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 9 cut(s) 174, 318, 621, 759, 773, 959, 981, 1056, 1104
HpySE526I ACGT 1 cut(s) 892
Hsp92II CATG 3 cut(s) 366, 668, 1168
Kzo9I GATC 2 cut(s) 947, 1190
LguI GCTCTTC 1 cut(s) 893
LmnI GCTCC 5 cut(s) 358, 509, 823, 844, 1318
Lsp1109I GCAGC 5 cut(s) 53, 337, 477, 1126, 1223
LweI GCATC 4 cut(s) 53, 436, 759, 1021
MaeI CTAG 3 cut(s) 567, 951, 1044
MaeII ACGT 1 cut(s) 892
MalI GATC 2 cut(s) 949, 1192
MboI GATC 2 cut(s) 947, 1190
MboII GAAGA 8 cut(s) 191, 304, 862, 865, 910, 1031, 1072, 1286
MflI RGATCY 1 cut(s) 1190
MhlI GDGCHC 1 cut(s) 1002
MluCI AATT 5 cut(s) 57, 358, 452, 688, 1134
MlyI GAGTC 1 cut(s) 1041
MmeI TCCRAC 1 cut(s) 569
MseI TTAA 3 cut(s) 396, 792, 1133
MslI CAYNNNNRTG 1 cut(s) 1254
MspA1I CMGCKG 2 cut(s) 350, 1268
MspR9I CCNGG 2 cut(s) 234, 261
Mva1269I GAATGC 2 cut(s) 28, 1162
MvaI CCWGG 2 cut(s) 234, 261
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeII GATC 2 cut(s) 947, 1190
NlaIII CATG 3 cut(s) 366, 668, 1168
NlaIV GGNNCC 6 cut(s) 10, 258, 265, 505, 825, 1000
NmeAIII GCCGAG 1 cut(s) 1252
NspI RCATGY 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 893
PctI GAATGC 2 cut(s) 28, 1162
PfeI GAWTC 2 cut(s) 511, 527
PkrI GCNGC 5 cut(s) 43, 352, 467, 1141, 1213
PleI GAGTC 1 cut(s) 1041
PpsI GAGTC 1 cut(s) 1041
PpuMI RGGWCCY 1 cut(s) 9
PshAI GACNNNNGTC 1 cut(s) 1051
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 232, 259
PspGI CCWGG 2 cut(s) 232, 259
PspN4I GGNNCC 6 cut(s) 10, 258, 265, 505, 825, 1000
PspOMI GGGCCC 1 cut(s) 998
PspPI GGNCC 6 cut(s) 9, 230, 256, 263, 998, 999
PspPPI RGGWCCY 1 cut(s) 9
PstI CTGCAG 3 cut(s) 43, 916, 1177
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 1190
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 978
RsaNI GTAC 1 cut(s) 977
RseI CAYNNNNRTG 1 cut(s) 1254
SapI GCTCTTC 1 cut(s) 893
SaqAI TTAA 3 cut(s) 396, 792, 1133
SatI GCNGC 5 cut(s) 42, 351, 466, 1140, 1212
Sau3AI GATC 2 cut(s) 947, 1190
Sau96I GGNCC 6 cut(s) 9, 230, 256, 263, 998, 999
SchI GAGTC 1 cut(s) 1041
ScrFI CCNGG 2 cut(s) 234, 261
SduI GDGCHC 1 cut(s) 1002
SfaNI GCATC 4 cut(s) 53, 436, 759, 1021
SfcI CTRYAG 4 cut(s) 39, 117, 912, 1173
SinI GGWCC 3 cut(s) 9, 230, 263
SmiMI CAYNNNNRTG 1 cut(s) 1254
Sse9I AATT 5 cut(s) 57, 358, 452, 688, 1134
SsiI CCGC 2 cut(s) 213, 1268
SspMI CTAG 3 cut(s) 567, 951, 1044
StyD4I CCNGG 2 cut(s) 232, 259
StyI CCWWGG 2 cut(s) 566, 1221
TaaI ACNGT 3 cut(s) 86, 157, 932
TaiI ACGT 1 cut(s) 895
TaqI TCGA 1 cut(s) 946
TasI AATT 5 cut(s) 57, 358, 452, 688, 1134
TfiI GAWTC 2 cut(s) 511, 527
Tru1I TTAA 3 cut(s) 396, 792, 1133
Tru9I TTAA 3 cut(s) 396, 792, 1133
TscAI CASTG 3 cut(s) 322, 994, 1177
TseI GCWGC 5 cut(s) 41, 350, 465, 1139, 1211
TspDTI ATGAA 6 cut(s) 17, 198, 305, 351, 627, 1287
TspGWI ACGGA 2 cut(s) 47, 1045
TspRI CASTG 3 cut(s) 322, 994, 1177
VpaK11BI GGWCC 3 cut(s) 9, 230, 263
XapI RAATTY 1 cut(s) 358
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XmaJI CCTAGG 1 cut(s) 566
XspI CTAG 3 cut(s) 567, 951, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.