RchiOBHm_Chr6g0297971

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004715
Physical Location & Seq
Forward (+)
59556740 .. 59561108
4369 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26746

Sequence Viewer

Length: 1374 bp
ATGGATTCGGATAATTTGGAAGCCGCCTATGGGCTTCAGGTTGCTCTTGAAAATGGTGGTCATGGGCAACTTAGTGTTGGTCCAGACGGCATTAATGGGAGTGCCGGTGAAACTACTCTTACCGAGACTGCAGCACCGAATGGGAGAATTGAAAATGTGGTGAACTCGGATGATGGTGTGTCTTATAATTCATCTGCTGGAGAAGTCAAAGAGGAATCCAGTGTCAACTCTGCAAGCAATGGTTTGACTATTGCTAAGGAAAGGGGACCGAAAGTTTCTGTTCAGTCAAAGCAATCCAGAGTGCAGAAGGGTCAAGGTAAGAGTAAGATTGAGAAGCCCCCTAGTCCCAAGAGCGCCTTGCCAATTCGGATGAAGAAAAGCAAAGATGGAAATGATGCAGAGGCTACAGCTACTGTTTCCAATGGTTCAGCAGGTCCAAATTCACTCGCCAAGCAGCCTAACAAGAGTAGATCATTGAATGGCCAACAGCAACCTAAACAATCTGAGGCACCATCGACTGAAGGCCTCGTGGAGGAGACAAATCTGAAGCCGCTAAACAAAGGCTCCTATAATAAGGCTGATGCAGACTCCCAATCTTCTTTAAGTCCCACGGAAGGAGATATGAAAGCACCTAGGGTGGGTACACTGCCAAAGTATGGTTTCAGCTTTAAGTGTGATGAAAGAGCTGAGAAAAGAAGAGAGTTCTACACCAAACTTGAGGAAAAGATACATGCAAAGGAGATGGAGAAGAATAATTTGCAAGCCAAGTCTAAGGAAACTCTAGAAGCTGAGATTAAGATGCTCAGGAAGAAGCTGACTTTTAAAGCAACTCCAATGCCAAGCTTTTATCAGGAGCCTCCGCCTCCTAAGGTGGAGCTAAAGAAGTTACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGCCGCAGGAAGAGCTTACCTCAAGCAGATTCTGGAGGAAACAGTAATACCAAGAGTCAATCTGGCAGGCTTAGTCTGGGTGAGAAACTTCCTCAAAACTCTGCCAAAGGACCTTCTCCTGTGCATCCAAAGAAGCCACAAAGAAAGTCTCTTCCTAGACTTCCATCTGAGAAGACCACTTTATCTAATGTGAAGAGTGTGCGGAAGGTTGCTTCTAAGGCAACAAACGAGGAAAAGGCTGCCGCGTTGCCCAATGCAACAAGTGAAGGTGGACCTCACACCCAAGAGCAGGAGGCAGTTCCCAGAGCTGAGATAACTGAACCCTACAGCCAAGAGCCGGAAGCAGTTCCCAGGGCTGCAATAAGTGAGGAGCAGTCCCATTCAGATGATGAGACAGTTAATGAAGAGGAACATCATCCCACCTTGGTTCAAGAGCCTATAGCGCTGGAGAATTGA

Protein Analysis

457

Amino Acids

49.18

Weight (kDa)

9.03

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 221 - 291 4.4e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 508
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 1 cut(s) 1163
AciI CCGC 6 cut(s) 24, 551, 860, 922, 1120, 1161
AcoI YGGCCR 2 cut(s) 481, 919
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 3 cut(s) 20, 540, 566
AfaI GTAC 1 cut(s) 643
AfeI AGCGCT 1 cut(s) 1362
AfiI CCNNNNNNNGG 8 cut(s) 30, 532, 614, 638, 656, 917, 1207, 1255
AgsI TTSAA 4 cut(s) 50, 152, 478, 1349
AjnI CCWGG 1 cut(s) 1268
Alw26I GTCTC 5 cut(s) 119, 530, 912, 1071, 1304
AlwNI CAGNNNCTG 2 cut(s) 413, 950
Aor51HI AGCGCT 1 cut(s) 1362
AoxI GGCC 3 cut(s) 481, 523, 919
ApeKI GCWGC 4 cut(s) 131, 454, 1157, 1274
ApoI RAATTY 1 cut(s) 439
AseI ATTAAT 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 1263
AspA2I CCTAGG 1 cut(s) 632
AspLEI GCGC 2 cut(s) 356, 1363
AspS9I GGNCC 5 cut(s) 80, 266, 434, 1028, 1190
AsuHPI GGTGA 3 cut(s) 119, 172, 1010
AvaII GGWCC 5 cut(s) 80, 266, 434, 1028, 1190
AvrII CCTAGG 1 cut(s) 632
AxyI CCTNAGG 1 cut(s) 867
BalI TGGCCA 1 cut(s) 483
BanI GGYRCC 1 cut(s) 508
BauI CACGAG 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 1097
BbvI GCAGC 4 cut(s) 143, 466, 1144, 1261
BccI CCATC 5 cut(s) 167, 380, 520, 736, 1090
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 1270
BcoDI GTCTC 5 cut(s) 119, 530, 912, 1071, 1304
BfaI CTAG 4 cut(s) 342, 633, 782, 1074
BfmI CTRYAG 4 cut(s) 129, 405, 1243, 1356
BfoI RGCGCY 2 cut(s) 357, 1364
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 8 cut(s) 24, 132, 455, 551, 922, 1158, 1161, 1275
BlnI CCTAGG 1 cut(s) 632
BlsI GCNGC 8 cut(s) 25, 133, 456, 552, 923, 1159, 1162, 1276
Bme1390I CCNGG 1 cut(s) 1270
Bme18I GGWCC 5 cut(s) 80, 266, 434, 1028, 1190
BmgT120I GGNCC 5 cut(s) 80, 266, 434, 1028, 1190
BmiI GGNNCC 4 cut(s) 267, 510, 565, 855
BmrFI CCNGG 1 cut(s) 1270
BmsI GCATC 4 cut(s) 385, 571, 789, 1051
BpiI GAAGAC 1 cut(s) 1097
BplI GAGNNNNNCTC 2 cut(s) 922, 954
BpmI CTGGAG 2 cut(s) 219, 972
Bpu10I CCTNAGC 2 cut(s) 255, 803
BpuEI CTTGAG 2 cut(s) 737, 924
BsaJI CCNNGG 5 cut(s) 609, 632, 1268, 1269, 1341
BsaXI ACNNNNNCTCC 4 cut(s) 91, 121, 548, 578
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 532, 614, 638, 656, 917, 1207, 1255
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 219
Bse21I CCTNAGG 1 cut(s) 867
Bse3DI GCAATG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 1270
BseDI CCNNGG 5 cut(s) 609, 632, 1268, 1269, 1341
BseGI GGATG 4 cut(s) 175, 375, 1042, 1333
BseLI CCNNNNNNNGG 8 cut(s) 30, 532, 614, 638, 656, 917, 1207, 1255
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 6 cut(s) 495, 678, 780, 817, 1077, 1218
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 2 cut(s) 548, 1301
BseX3I CGGCCG 1 cut(s) 919
BseXI GCAGC 4 cut(s) 143, 466, 1144, 1261
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 1 cut(s) 1163
Bsh1285I CGRYCG 1 cut(s) 922
BshFI GGCC 3 cut(s) 483, 525, 921
BshNI GGYRCC 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 922
BsiSI CCGG 2 cut(s) 105, 1256
BslFI GGGAC 4 cut(s) 279, 330, 591, 1279
BslI CCNNNNNNNGG 8 cut(s) 30, 532, 614, 638, 656, 917, 1207, 1255
BsmAI GTCTC 5 cut(s) 119, 530, 912, 1071, 1304
BsmFI GGGAC 4 cut(s) 279, 330, 591, 1279
BsnI GGCC 3 cut(s) 483, 525, 921
Bsp143I GATC 1 cut(s) 470
BspACI CCGC 6 cut(s) 24, 551, 860, 922, 1120, 1161
BspANI GGCC 3 cut(s) 483, 525, 921
BspCNI CTCAG 6 cut(s) 496, 679, 781, 816, 1078, 1219
BspFNI CGCG 1 cut(s) 1163
BspLI GGNNCC 4 cut(s) 267, 510, 565, 855
BspMAI CTGCAG 1 cut(s) 133
BspMI ACCTGC 1 cut(s) 422
BspQI GCTCTTC 1 cut(s) 923
BspT107I GGYRCC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 1 cut(s) 219
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 609, 632, 1268, 1269, 1341
BssMI GATC 1 cut(s) 470
BssSI CACGAG 1 cut(s) 527
BssT1I CCWWGG 2 cut(s) 632, 1341
Bst2BI CACGAG 1 cut(s) 527
Bst2UI CCWGG 1 cut(s) 1270
Bst4CI ACNGT 3 cut(s) 415, 962, 1315
Bst6I CTCTTC 5 cut(s) 691, 923, 1074, 1106, 1317
BstC8I GCNNGC 3 cut(s) 235, 762, 986
BstDSI CCRYGG 1 cut(s) 609
BstENI CCTNNNNNAGG 1 cut(s) 530
BstF5I GGATG 4 cut(s) 175, 375, 1042, 1333
BstFNI CGCG 1 cut(s) 1163
BstH2I RGCGCY 2 cut(s) 357, 1364
BstHHI GCGC 2 cut(s) 356, 1363
BstKTI GATC 1 cut(s) 473
BstMAI GTCTC 5 cut(s) 119, 530, 912, 1071, 1304
BstMBI GATC 1 cut(s) 470
BstMCI CGRYCG 1 cut(s) 922
BstMWI GCNNNNNNNGC 3 cut(s) 930, 1136, 1360
BstNI CCWGG 1 cut(s) 1270
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 1268
BstSFI CTRYAG 4 cut(s) 129, 405, 1243, 1356
BstUI CGCG 1 cut(s) 1163
BstV1I GCAGC 4 cut(s) 143, 466, 1144, 1261
BstV2I GAAGAC 1 cut(s) 1097
BstZI CGGCCG 1 cut(s) 919
Bsu36I CCTNAGG 1 cut(s) 867
BsuRI GGCC 3 cut(s) 483, 525, 921
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 4 cut(s) 175, 375, 1042, 1333
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 2 cut(s) 226, 644
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 3 cut(s) 235, 762, 986
CaiI CAGNNNCTG 2 cut(s) 413, 950
CfoI GCGC 2 cut(s) 356, 1363
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 80, 266, 434, 1028, 1190
Csp6I GTAC 1 cut(s) 642
CviAII CATG 2 cut(s) 62, 731
CviQI GTAC 1 cut(s) 642
DpnI GATC 1 cut(s) 472
DpnII GATC 1 cut(s) 470
DraI TTTAAA 1 cut(s) 823
EaeI YGGCCR 2 cut(s) 481, 919
EagI CGGCCG 1 cut(s) 919
Eam1104I CTCTTC 5 cut(s) 691, 923, 1074, 1106, 1317
EarI CTCTTC 5 cut(s) 691, 923, 1074, 1106, 1317
EciI GGCGGA 1 cut(s) 849
EclXI CGGCCG 1 cut(s) 919
Eco130I CCWWGG 2 cut(s) 632, 1341
Eco147I AGGCCT 1 cut(s) 525
Eco47I GGWCC 5 cut(s) 80, 266, 434, 1028, 1190
Eco47III AGCGCT 1 cut(s) 1362
Eco52I CGGCCG 1 cut(s) 919
Eco57I CTGAAG 3 cut(s) 20, 540, 566
Eco81I CCTNAGG 1 cut(s) 867
EcoNI CCTNNNNNAGG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 1268
EcoT14I CCWWGG 2 cut(s) 632, 1341
ErhI CCWWGG 2 cut(s) 632, 1341
FaeI CATG 2 cut(s) 65, 734
FaiI YATR 8 cut(s) 30, 63, 186, 570, 623, 657, 732, 1358
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 4 cut(s) 279, 330, 591, 1279
FatI CATG 2 cut(s) 61, 730
Fnu4HI GCNGC 8 cut(s) 24, 132, 455, 551, 922, 1158, 1161, 1275
FokI GGATG 4 cut(s) 182, 382, 1029, 1320
Fsp4HI GCNGC 8 cut(s) 24, 132, 455, 551, 922, 1158, 1161, 1275
FspBI CTAG 4 cut(s) 342, 633, 782, 1074
GlaI GCGC 2 cut(s) 355, 1362
GluI GCNGC 8 cut(s) 24, 132, 455, 551, 922, 1158, 1161, 1275
GsuI CTGGAG 2 cut(s) 219, 972
HaeII RGCGCY 2 cut(s) 357, 1364
HaeIII GGCC 3 cut(s) 483, 525, 921
HapII CCGG 2 cut(s) 105, 1256
HhaI GCGC 2 cut(s) 356, 1363
Hin1II CATG 2 cut(s) 65, 734
Hin6I GCGC 2 cut(s) 354, 1361
HinP1I GCGC 2 cut(s) 354, 1361
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 841
HinfI GANTC 5 cut(s) 5, 215, 587, 947, 973
HpaII CCGG 2 cut(s) 105, 1256
HphI GGTGA 3 cut(s) 119, 172, 1010
Hpy166II GTNNAC 4 cut(s) 163, 226, 644, 1190
Hpy188I TCNGA 7 cut(s) 10, 169, 369, 505, 546, 1087, 1303
Hpy188III TCNNGA 8 cut(s) 47, 83, 297, 782, 805, 851, 951, 1349
Hpy8I GTNNAC 4 cut(s) 163, 226, 644, 1190
HpyAV CCTTC 6 cut(s) 301, 515, 608, 1041, 1117, 1178
HpyCH4III ACNGT 3 cut(s) 415, 962, 1315
HpyF10VI GCNNNNNNNGC 3 cut(s) 930, 1136, 1360
Hsp92II CATG 2 cut(s) 65, 734
HspAI GCGC 2 cut(s) 354, 1361
Kzo9I GATC 1 cut(s) 470
LguI GCTCTTC 1 cut(s) 923
LmnI GCTCC 4 cut(s) 569, 853, 874, 1288
Lsp1109I GCAGC 4 cut(s) 143, 466, 1144, 1261
LweI GCATC 4 cut(s) 385, 571, 789, 1051
MaeI CTAG 4 cut(s) 342, 633, 782, 1074
MaeIII GTNAC 1 cut(s) 885
MalI GATC 1 cut(s) 472
MboI GATC 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 7 cut(s) 13, 147, 187, 363, 439, 754, 1369
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 2 cut(s) 581, 982
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 1263
MscI TGGCCA 1 cut(s) 483
MseI TTAA 6 cut(s) 93, 602, 669, 795, 822, 1317
MslI CAYNNNNRTG 1 cut(s) 1302
Msp20I TGGCCA 1 cut(s) 483
MspI CCGG 2 cut(s) 105, 1256
MspR9I CCNGG 1 cut(s) 1270
MvaI CCWGG 1 cut(s) 1270
MvnI CGCG 1 cut(s) 1163
MwoI GCNNNNNNNGC 3 cut(s) 930, 1136, 1360
NdeII GATC 1 cut(s) 470
NlaIII CATG 2 cut(s) 65, 734
NlaIV GGNNCC 4 cut(s) 267, 510, 565, 855
NmeAIII GCCGAG 1 cut(s) 897
NspI RCATGY 1 cut(s) 734
PasI CCCWGGG 1 cut(s) 1269
PceI AGGCCT 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 923
PdmI GAANNNNTTC 1 cut(s) 1263
PfeI GAWTC 3 cut(s) 5, 215, 947
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 8 cut(s) 25, 133, 456, 552, 923, 1159, 1162, 1276
PleI GAGTC 2 cut(s) 581, 981
PpsI GAGTC 2 cut(s) 581, 981
PpuMI RGGWCCY 1 cut(s) 1028
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 1028
Psp6I CCWGG 1 cut(s) 1268
PspGI CCWGG 1 cut(s) 1268
PspN4I GGNNCC 4 cut(s) 267, 510, 565, 855
PspPI GGNCC 5 cut(s) 80, 266, 434, 1028, 1190
PspPPI RGGWCCY 1 cut(s) 1028
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 2 cut(s) 413, 950
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
RseI CAYNNNNRTG 1 cut(s) 1302
SapI GCTCTTC 1 cut(s) 923
SaqAI TTAA 6 cut(s) 93, 602, 669, 795, 822, 1317
SatI GCNGC 8 cut(s) 24, 132, 455, 551, 922, 1158, 1161, 1275
Sau3AI GATC 1 cut(s) 470
Sau96I GGNCC 5 cut(s) 80, 266, 434, 1028, 1190
SchI GAGTC 2 cut(s) 581, 982
ScrFI CCNGG 1 cut(s) 1270
SfaNI GCATC 4 cut(s) 385, 571, 789, 1051
SfcI CTRYAG 4 cut(s) 129, 405, 1243, 1356
SinI GGWCC 5 cut(s) 80, 266, 434, 1028, 1190
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 2 cut(s) 716, 939
SmoI CTYRAG 2 cut(s) 716, 939
Sse9I AATT 7 cut(s) 13, 147, 187, 363, 439, 754, 1369
SseBI AGGCCT 1 cut(s) 525
SsiI CCGC 6 cut(s) 24, 551, 860, 922, 1120, 1161
SspMI CTAG 4 cut(s) 342, 633, 782, 1074
StuI AGGCCT 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 1268
StyI CCWWGG 2 cut(s) 632, 1341
TaaI ACNGT 3 cut(s) 415, 962, 1315
TaqI TCGA 1 cut(s) 515
TaqII GACCGA 1 cut(s) 283
TasI AATT 7 cut(s) 13, 147, 187, 363, 439, 754, 1369
TauI GCSGC 4 cut(s) 26, 553, 924, 1163
TfiI GAWTC 3 cut(s) 5, 215, 947
Tru1I TTAA 6 cut(s) 93, 602, 669, 795, 822, 1317
Tru9I TTAA 6 cut(s) 93, 602, 669, 795, 822, 1317
TscAI CASTG 2 cut(s) 226, 651
TseI GCWGC 4 cut(s) 131, 454, 1157, 1274
TspDTI ATGAA 5 cut(s) 180, 386, 638, 693, 1335
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 2 cut(s) 226, 651
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 5 cut(s) 80, 266, 434, 1028, 1190
VspI ATTAAT 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 530
XapI RAATTY 1 cut(s) 439
XbaI TCTAGA 1 cut(s) 781
XceI RCATGY 1 cut(s) 734
XmaJI CCTAGG 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 1263
XspI CTAG 4 cut(s) 342, 633, 782, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.