RH6DG388300

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0004715
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG388300.1

Sequence Viewer

Length: 1371 bp
ATGGATTCGGATAATTTGGAAGCCGCCTATGGGCTTCAGGTTGCTCTTGAAAATGGTGGTCATGGGCAACTTAGTGTTGGTCCAGACGGTGAAACTACTCTCACCGAGACTGCAGCACCGAATGGGAGAATTGAAAATGTGGTGAACTCAGATGATGGTGTGTCGTATAATTCATCTGCTGGAGAAGTCAAAGAGGAATCCAGTGTCAACTCTGCAAGCAATGGTTTGACTATTGCTAAGGAAAGGGGACCGAAAGTTTCTGTTCAGTCAAAGCAATCCAAAGTGCAGAAGGGTCAAGGTAAGAGTAAGATTGAGAAGCCCCCTAGTCCCAAGAGCGCCTTGCCAATTCGGATGAAGAAAAGCAAAGATGGAAATGATGCAGAGGCTACAGCTACTGTTTCCAATGGTTCAGCAGGTCCAAATTCACTCGCCAAGCAGCCTAACAAGAGTAGATCATTGAATGGCCAACAGGTTCAGTTATCCAATCAACCTAAACAATCTGAGGCACCATCGACTGAAGGCCTTGTGGAGGAGACAAATCTGAAGCCACTAAACAAAGGCTCCTATAATAAGGCTGATGGAGACTCCCAATCTTCTTTAAGTCCCACGGAAGGAGATATGAAAGCACCTAGGGTGGGTACACTGCCAAAGTATGGTTTCAGCTTTAAGTGTGATGAAAGAGCTGAGAAAAGAAGAGAGTTCTACACCAAACTTGAGGAAAAGATACATGCAAAGGAGATGGAGAAGAATAATTTGCAAGCCAAGTCTAAGGAAACTCTAGAAGCTGAGATTAAGATGCTCAGGAAGAAGCTGACTTTTAAAGCAACTCCAATGCCAAGCTTTTATCAGGAGCCTCCGCCTCCTAAGGTGGAGCTAAAGAAGTTACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGCCGCAGGAAGAGCTTACCTCCAGCAGATTCTGAAGGAAACAGTAATACCAAGAGTCAATCTGGCAGGCTTAGTCTGGGTGAGAAACTTCCTCAAAACTCTGCCAAAGGACCTTCTCCTGTGCATCCAAAGAAGCCACAAAGAAAGTCTCTTCCTAGACTTCCATCTGAGAAGACCACTTTATCTAATGTGAAGAGTGTGCGGAAGGTTACTTCTAAGGCAACAAACGAGGAAAAGGCTGCCGTGTTGCCCAATGCAACAAGTGAAGCTGGACCTCACACCCGAGAGCAGGAGGCAGTTCCCAGAGCTGAGATAACTGAACCCTACACCCAAGAGCCGGAAGCAGTTCCCAGGGCTGCAATAAGTGAGGAGCAGTCCCATTCAGATGATGAGACAGTTAATGAAGAGGAACATCATCCCACCTTGGTTCAAGAGCCTATAGCGCTGGAGAATTGA

Protein Analysis

456

Amino Acids

49.33

Weight (kDa)

9.03

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 220 - 290 4.4e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 653
AciI CCGC 4 cut(s) 24, 857, 919, 1117
AcoI YGGCCR 2 cut(s) 463, 916
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 4 cut(s) 20, 537, 563, 969
AfaI GTAC 1 cut(s) 640
AfeI AGCGCT 1 cut(s) 1359
AfiI CCNNNNNNNGG 8 cut(s) 30, 529, 611, 635, 653, 914, 1204, 1252
AgsI TTSAA 4 cut(s) 50, 134, 460, 1346
AjnI CCWGG 1 cut(s) 1265
Alw26I GTCTC 6 cut(s) 101, 527, 576, 909, 1068, 1301
AlwNI CAGNNNCTG 2 cut(s) 395, 947
Ama87I CYCGRG 1 cut(s) 1197
Aor51HI AGCGCT 1 cut(s) 1359
AoxI GGCC 3 cut(s) 463, 520, 916
ApeKI GCWGC 4 cut(s) 113, 436, 1154, 1271
ApoI RAATTY 1 cut(s) 421
Asp700I GAANNNNTTC 1 cut(s) 1260
AspA2I CCTAGG 1 cut(s) 629
AspLEI GCGC 2 cut(s) 338, 1360
AspS9I GGNCC 5 cut(s) 80, 248, 416, 1025, 1187
AsuHPI GGTGA 4 cut(s) 94, 101, 154, 1007
AvaI CYCGRG 1 cut(s) 1197
AvaII GGWCC 5 cut(s) 80, 248, 416, 1025, 1187
AvrII CCTAGG 1 cut(s) 629
AxyI CCTNAGG 1 cut(s) 864
BalI TGGCCA 1 cut(s) 465
BanI GGYRCC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 1094
BbvI GCAGC 4 cut(s) 125, 448, 1141, 1258
BccI CCATC 6 cut(s) 149, 362, 517, 572, 733, 1087
BceAI ACGGC 1 cut(s) 1142
BciT130I CCWGG 1 cut(s) 1267
BcoDI GTCTC 6 cut(s) 101, 527, 576, 909, 1068, 1301
BfaI CTAG 4 cut(s) 324, 630, 779, 1071
BfmI CTRYAG 3 cut(s) 111, 387, 1353
BfoI RGCGCY 2 cut(s) 339, 1361
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 6 cut(s) 24, 114, 437, 919, 1155, 1272
BlnI CCTAGG 1 cut(s) 629
BlsI GCNGC 6 cut(s) 25, 115, 438, 920, 1156, 1273
Bme1390I CCNGG 1 cut(s) 1267
Bme18I GGWCC 5 cut(s) 80, 248, 416, 1025, 1187
BmeT110I CYCGRG 1 cut(s) 1197
BmgT120I GGNCC 5 cut(s) 80, 248, 416, 1025, 1187
BmiI GGNNCC 4 cut(s) 249, 507, 562, 852
BmrFI CCNGG 1 cut(s) 1267
BmsI GCATC 3 cut(s) 367, 786, 1048
BpiI GAAGAC 1 cut(s) 1094
BplI GAGNNNNNCTC 2 cut(s) 919, 951
BpmI CTGGAG 2 cut(s) 201, 921
Bpu10I CCTNAGC 2 cut(s) 237, 800
BpuEI CTTGAG 1 cut(s) 734
BsaJI CCNNGG 5 cut(s) 606, 629, 1265, 1266, 1338
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 529, 611, 635, 653, 914, 1204, 1252
Bse1I ACTGG 1 cut(s) 201
Bse21I CCTNAGG 1 cut(s) 864
Bse3DI GCAATG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 1267
BseDI CCNNGG 5 cut(s) 606, 629, 1265, 1266, 1338
BseGI GGATG 3 cut(s) 357, 1039, 1330
BseLI CCNNNNNNNGG 8 cut(s) 30, 529, 611, 635, 653, 914, 1204, 1252
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 7 cut(s) 162, 492, 675, 777, 814, 1074, 1215
BseNI ACTGG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 545, 1298
BseX3I CGGCCG 1 cut(s) 916
BseXI GCAGC 4 cut(s) 125, 448, 1141, 1258
BsgI GTGCAG 1 cut(s) 305
Bsh1285I CGRYCG 1 cut(s) 919
BshFI GGCC 3 cut(s) 465, 522, 918
BshNI GGYRCC 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 919
BsiHKCI CYCGRG 1 cut(s) 1197
BsiSI CCGG 1 cut(s) 1253
BslFI GGGAC 4 cut(s) 261, 312, 588, 1276
BslI CCNNNNNNNGG 8 cut(s) 30, 529, 611, 635, 653, 914, 1204, 1252
BsmAI GTCTC 6 cut(s) 101, 527, 576, 909, 1068, 1301
BsmFI GGGAC 4 cut(s) 261, 312, 588, 1276
BsnI GGCC 3 cut(s) 465, 522, 918
BsoBI CYCGRG 1 cut(s) 1197
Bsp143I GATC 1 cut(s) 452
BspACI CCGC 4 cut(s) 24, 857, 919, 1117
BspANI GGCC 3 cut(s) 465, 522, 918
BspCNI CTCAG 7 cut(s) 161, 493, 676, 778, 813, 1075, 1216
BspLI GGNNCC 4 cut(s) 249, 507, 562, 852
BspMAI CTGCAG 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 404
BspQI GCTCTTC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 5 cut(s) 606, 629, 1265, 1266, 1338
BssMI GATC 1 cut(s) 452
BssT1I CCWWGG 2 cut(s) 629, 1338
Bst2UI CCWGG 1 cut(s) 1267
Bst4CI ACNGT 4 cut(s) 89, 397, 959, 1312
Bst6I CTCTTC 5 cut(s) 688, 920, 1071, 1103, 1314
BstC8I GCNNGC 3 cut(s) 217, 759, 983
BstDSI CCRYGG 1 cut(s) 606
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 3 cut(s) 357, 1039, 1330
BstH2I RGCGCY 2 cut(s) 339, 1361
BstHHI GCGC 2 cut(s) 338, 1360
BstKTI GATC 1 cut(s) 455
BstMAI GTCTC 6 cut(s) 101, 527, 576, 909, 1068, 1301
BstMBI GATC 1 cut(s) 452
BstMCI CGRYCG 1 cut(s) 919
BstMWI GCNNNNNNNGC 2 cut(s) 927, 1357
BstNI CCWGG 1 cut(s) 1267
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 1 cut(s) 1265
BstSFI CTRYAG 3 cut(s) 111, 387, 1353
BstV1I GCAGC 4 cut(s) 125, 448, 1141, 1258
BstV2I GAAGAC 1 cut(s) 1094
BstZI CGGCCG 1 cut(s) 916
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 3 cut(s) 465, 522, 918
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 3 cut(s) 357, 1039, 1330
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 2 cut(s) 208, 641
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 217, 759, 983
CaiI CAGNNNCTG 2 cut(s) 395, 947
CfoI GCGC 2 cut(s) 338, 1360
Cfr13I GGNCC 5 cut(s) 80, 248, 416, 1025, 1187
Csp6I GTAC 1 cut(s) 639
CviAII CATG 2 cut(s) 62, 728
CviQI GTAC 1 cut(s) 639
DpnI GATC 1 cut(s) 454
DpnII GATC 1 cut(s) 452
DraI TTTAAA 1 cut(s) 820
EaeI YGGCCR 2 cut(s) 463, 916
EagI CGGCCG 1 cut(s) 916
Eam1104I CTCTTC 5 cut(s) 688, 920, 1071, 1103, 1314
EarI CTCTTC 5 cut(s) 688, 920, 1071, 1103, 1314
EciI GGCGGA 1 cut(s) 846
EclXI CGGCCG 1 cut(s) 916
Eco130I CCWWGG 2 cut(s) 629, 1338
Eco147I AGGCCT 1 cut(s) 522
Eco47I GGWCC 5 cut(s) 80, 248, 416, 1025, 1187
Eco47III AGCGCT 1 cut(s) 1359
Eco52I CGGCCG 1 cut(s) 916
Eco57I CTGAAG 4 cut(s) 20, 537, 563, 969
Eco81I CCTNAGG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 1197
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 1 cut(s) 1025
EcoRII CCWGG 1 cut(s) 1265
EcoT14I CCWWGG 2 cut(s) 629, 1338
ErhI CCWWGG 2 cut(s) 629, 1338
FaeI CATG 2 cut(s) 65, 731
FaiI YATR 8 cut(s) 30, 63, 168, 567, 620, 654, 729, 1355
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 4 cut(s) 261, 312, 588, 1276
FatI CATG 2 cut(s) 61, 727
Fnu4HI GCNGC 6 cut(s) 24, 114, 437, 919, 1155, 1272
FokI GGATG 3 cut(s) 364, 1026, 1317
Fsp4HI GCNGC 6 cut(s) 24, 114, 437, 919, 1155, 1272
FspBI CTAG 4 cut(s) 324, 630, 779, 1071
GlaI GCGC 2 cut(s) 337, 1359
GluI GCNGC 6 cut(s) 24, 114, 437, 919, 1155, 1272
GsuI CTGGAG 2 cut(s) 201, 921
HaeII RGCGCY 2 cut(s) 339, 1361
HaeIII GGCC 3 cut(s) 465, 522, 918
HapII CCGG 1 cut(s) 1253
HhaI GCGC 2 cut(s) 338, 1360
Hin1II CATG 2 cut(s) 65, 731
Hin6I GCGC 2 cut(s) 336, 1358
HinP1I GCGC 2 cut(s) 336, 1358
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 838
HinfI GANTC 5 cut(s) 5, 197, 584, 944, 970
HpaII CCGG 1 cut(s) 1253
HphI GGTGA 4 cut(s) 94, 101, 154, 1007
Hpy166II GTNNAC 3 cut(s) 145, 208, 641
Hpy188I TCNGA 8 cut(s) 10, 151, 351, 502, 543, 949, 1084, 1300
Hpy188III TCNNGA 6 cut(s) 47, 83, 779, 802, 848, 1346
Hpy8I GTNNAC 3 cut(s) 145, 208, 641
HpyAV CCTTC 6 cut(s) 283, 512, 605, 944, 1038, 1114
HpyCH4III ACNGT 4 cut(s) 89, 397, 959, 1312
HpyCH4V TGCA 9 cut(s) 113, 215, 286, 380, 731, 757, 1039, 1172, 1274
HpyF10VI GCNNNNNNNGC 2 cut(s) 927, 1357
Hsp92II CATG 2 cut(s) 65, 731
HspAI GCGC 2 cut(s) 336, 1358
Kzo9I GATC 1 cut(s) 452
LguI GCTCTTC 1 cut(s) 920
LmnI GCTCC 4 cut(s) 566, 850, 871, 1285
Lsp1109I GCAGC 4 cut(s) 125, 448, 1141, 1258
LweI GCATC 3 cut(s) 367, 786, 1048
MaeI CTAG 4 cut(s) 324, 630, 779, 1071
MaeIII GTNAC 2 cut(s) 882, 1123
MalI GATC 1 cut(s) 454
MboI GATC 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 465
MluCI AATT 7 cut(s) 13, 129, 169, 345, 421, 751, 1366
MluNI TGGCCA 1 cut(s) 465
MlyI GAGTC 2 cut(s) 578, 979
Mox20I TGGCCA 1 cut(s) 465
MroXI GAANNNNTTC 1 cut(s) 1260
MscI TGGCCA 1 cut(s) 465
MseI TTAA 5 cut(s) 599, 666, 792, 819, 1314
MslI CAYNNNNRTG 1 cut(s) 1299
Msp20I TGGCCA 1 cut(s) 465
MspI CCGG 1 cut(s) 1253
MspR9I CCNGG 1 cut(s) 1267
MvaI CCWGG 1 cut(s) 1267
MwoI GCNNNNNNNGC 2 cut(s) 927, 1357
NdeII GATC 1 cut(s) 452
NlaIII CATG 2 cut(s) 65, 731
NlaIV GGNNCC 4 cut(s) 249, 507, 562, 852
NmeAIII GCCGAG 1 cut(s) 894
NspI RCATGY 1 cut(s) 731
PasI CCCWGGG 1 cut(s) 1266
PceI AGGCCT 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 920
PdmI GAANNNNTTC 1 cut(s) 1260
PfeI GAWTC 3 cut(s) 5, 197, 944
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 6 cut(s) 25, 115, 438, 920, 1156, 1273
PleI GAGTC 2 cut(s) 578, 978
PpsI GAGTC 2 cut(s) 578, 978
PpuMI RGGWCCY 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 1 cut(s) 1265
PspGI CCWGG 1 cut(s) 1265
PspN4I GGNNCC 4 cut(s) 249, 507, 562, 852
PspPI GGNCC 5 cut(s) 80, 248, 416, 1025, 1187
PspPPI RGGWCCY 1 cut(s) 1025
PstI CTGCAG 1 cut(s) 115
PstNI CAGNNNCTG 2 cut(s) 395, 947
RsaI GTAC 1 cut(s) 640
RsaNI GTAC 1 cut(s) 639
RseI CAYNNNNRTG 1 cut(s) 1299
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 5 cut(s) 599, 666, 792, 819, 1314
SatI GCNGC 6 cut(s) 24, 114, 437, 919, 1155, 1272
Sau3AI GATC 1 cut(s) 452
Sau96I GGNCC 5 cut(s) 80, 248, 416, 1025, 1187
SchI GAGTC 2 cut(s) 578, 979
ScrFI CCNGG 1 cut(s) 1267
SfaNI GCATC 3 cut(s) 367, 786, 1048
SfcI CTRYAG 3 cut(s) 111, 387, 1353
SinI GGWCC 5 cut(s) 80, 248, 416, 1025, 1187
SmiMI CAYNNNNRTG 1 cut(s) 1299
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 7 cut(s) 13, 129, 169, 345, 421, 751, 1366
SseBI AGGCCT 1 cut(s) 522
SsiI CCGC 4 cut(s) 24, 857, 919, 1117
SspMI CTAG 4 cut(s) 324, 630, 779, 1071
StuI AGGCCT 1 cut(s) 522
StyD4I CCNGG 1 cut(s) 1265
StyI CCWWGG 2 cut(s) 629, 1338
TaaI ACNGT 4 cut(s) 89, 397, 959, 1312
TaqI TCGA 1 cut(s) 512
TaqII GACCGA 1 cut(s) 265
TasI AATT 7 cut(s) 13, 129, 169, 345, 421, 751, 1366
TauI GCSGC 2 cut(s) 26, 921
TfiI GAWTC 3 cut(s) 5, 197, 944
Tru1I TTAA 5 cut(s) 599, 666, 792, 819, 1314
Tru9I TTAA 5 cut(s) 599, 666, 792, 819, 1314
TscAI CASTG 2 cut(s) 208, 648
TseI GCWGC 4 cut(s) 113, 436, 1154, 1271
TspDTI ATGAA 5 cut(s) 162, 368, 635, 690, 1332
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 208, 648
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 5 cut(s) 80, 248, 416, 1025, 1187
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 1 cut(s) 421
XbaI TCTAGA 1 cut(s) 778
XceI RCATGY 1 cut(s) 731
XmaJI CCTAGG 1 cut(s) 629
XmnI GAANNNNTTC 1 cut(s) 1260
XspI CTAG 4 cut(s) 324, 630, 779, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.