MD15G1125300.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004715
Physical Location & Seq
Reverse (-)
9098693 .. 9103108
4416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1125300.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGAATGGGACCTTCAGCGAGAATACTATGACCGATACTGCAGCATCAAACGGGAAAATTGAAAATGTTGATAAGTTGGATGATGGTGTTACTAATAGCTCATCTATTTGGAAAGCCAAAGAGGCCAATGTCAACCCTGAAAGCAATGGTTTGACTGTTGCTTTGACTATTGCTAAGGAAGACGAAGTGAAAGGTTCTACTCACCCAAAGCCCGCCAAAGTGCATAAAGGTCAAGGGAAGAGCAAGAATGAGAAGGCCCCTGGTGCCAAGAGCATCTCCCCAATTTCGATGAAGAAAAGCAAAGATGGAAATGGCGCAGAGGCAAAAGCTTCTGTTTCAAATGGTTCAGCTGCTCCAAATTCACGCCCCAAACAGCCTAACAAGAGTAGATCATTTAATGGGCGACAGGTTCAGTCATCCAATCAACCTGAAAAACCTGATGCAGAATTGTCTGAAAGCAGCGTGGAGAAGGCAAAGCTGAAGCCTTTGAAAAAGGGATCCCAGAATCAAGCTGAAGGAGAATCTCAATCCTCTTTAAGTCCTACAGAAGGAGATAAACATCCTAGGGTTGGTACACTTCCGAACTATGGTTTCAGTTTTAGGTGCGATGAACGAGCTGAGAAAAGGAGAGAGTTCTATACGAAACTGGAGGAAAAGATACATGCAAAGGAGATGGAAAAGAATAATTTGCAAGCCAAGTCTAAGGTGGTATTCAATCAATGGTGGTCAAGGGAAACCTCTGAAACTCTAGAAGCTGAGATTAGGATGCTCAGGAAGAAACTGACTTTCAAAGCAACTCCAATGCCAAGCTTCTATCAGGAGCCTCCACCTCCTAAGGTGGAGCTGAAGAAGATACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGACGTAGGAACAGCTTACCTCCTGCAGTTTCTGAAGCAAAGGGTAATACAAATGGTCAATCTAGCAGGCTTAGTCTGGATCAGAAAGTCCCTCAGAACACTTCTAAAGGGCCCTCCCCTGTGCATCCAAAGAAGCCACAACGGAAATCTCTTCCTAGACTCCCATCTGAGAAGACCACTTTACCTAATGCAGTGAATGAACGAAAAATGACTTTGAAGGCAGTAAACGAGGAAAAGACCAACTTAATTGCTGCAATGAATGAGAATGCCACCGTGCCCACTGCAGAAAGTGAAACTGGATCTCACACCCAAGATCAGGAGGCAGCAGTTCCCAAGGCCAAGGTAAGCGAAGAGCAGTCCCATACAGATGACGAAACAGCCGATGAAGAGCAACATCAGCCCACCTACATGCAAGAACCTATAGCCTCGGAGCATTGA

Protein Analysis

441

Amino Acids

48.27

Weight (kDa)

9.39

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 198 - 285 4.5e-20 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AciI CCGC 1 cut(s) 213
AclWI GGATC 4 cut(s) 492, 505, 972, 1192
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 4 cut(s) 500, 534, 866, 939
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 5 cut(s) 548, 569, 587, 884, 1201
AgsI TTSAA 6 cut(s) 62, 339, 490, 715, 790, 1102
AjnI CCWGG 1 cut(s) 259
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 4 cut(s) 492, 505, 972, 1192
AlwNI CAGNNNCTG 1 cut(s) 917
AoxI GGCC 4 cut(s) 123, 255, 995, 1221
ApaI GGGCCC 1 cut(s) 999
ApeKI GCWGC 5 cut(s) 41, 350, 459, 1136, 1208
ApoI RAATTY 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 145, 177
AspA2I CCTAGG 1 cut(s) 563
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 4 cut(s) 9, 256, 995, 996
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 9
AvrII CCTAGG 1 cut(s) 563
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 2 cut(s) 999, 1164
BamHI GGATCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 2 cut(s) 186, 1064
BbvI GCAGC 5 cut(s) 53, 337, 471, 1123, 1220
BccI CCATC 4 cut(s) 77, 299, 667, 1057
BciT130I CCWGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 879
BfaI CTAG 4 cut(s) 564, 749, 948, 1041
BfmI CTRYAG 5 cut(s) 39, 543, 909, 1167, 1305
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 5 cut(s) 42, 351, 460, 1137, 1209
BlnI CCTAGG 1 cut(s) 563
BlsI GCNGC 5 cut(s) 43, 352, 461, 1138, 1210
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 9
BmgT120I GGNCC 4 cut(s) 9, 256, 995, 996
BmiI GGNNCC 6 cut(s) 10, 258, 265, 499, 822, 997
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 5 cut(s) 53, 282, 430, 756, 1018
BpiI GAAGAC 2 cut(s) 186, 1064
BpmI CTGGAG 1 cut(s) 668
Bpu10I CCTNAGC 2 cut(s) 174, 770
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 5 cut(s) 259, 563, 1218, 1224, 1311
Bsc4I CCNNNNNNNGG 5 cut(s) 548, 569, 587, 884, 1201
Bse1I ACTGG 2 cut(s) 651, 1186
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 2 cut(s) 151, 1146
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 5 cut(s) 259, 563, 1218, 1224, 1311
BseGI GGATG 5 cut(s) 85, 416, 559, 771, 1009
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 5 cut(s) 548, 569, 587, 884, 1201
BseMI GCAATG 2 cut(s) 151, 1146
BseMII CTCAG 5 cut(s) 609, 747, 784, 992, 1044
BseNI ACTGG 2 cut(s) 651, 1186
BseSI GKGCMC 2 cut(s) 999, 1164
BseXI GCAGC 5 cut(s) 53, 337, 471, 1123, 1220
BshFI GGCC 4 cut(s) 125, 257, 997, 1223
BshNI GGYRCC 1 cut(s) 263
BslFI GGGAC 3 cut(s) 22, 959, 1228
BslI CCNNNNNNNGG 5 cut(s) 548, 569, 587, 884, 1201
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 3 cut(s) 22, 959, 1228
BsmI GAATGC 1 cut(s) 1156
BsnI GGCC 4 cut(s) 125, 257, 997, 1223
Bsp120I GGGCCC 1 cut(s) 995
Bsp1286I GDGCHC 2 cut(s) 999, 1164
Bsp143I GATC 5 cut(s) 389, 497, 964, 1184, 1198
BspACI CCGC 1 cut(s) 213
BspANI GGCC 4 cut(s) 125, 257, 997, 1223
BspCNI CTCAG 5 cut(s) 610, 748, 783, 991, 1045
BspLI GGNNCC 6 cut(s) 10, 258, 265, 499, 822, 997
BspMAI CTGCAG 3 cut(s) 43, 913, 1171
BspPI GGATC 4 cut(s) 492, 505, 972, 1192
BspQI GCTCTTC 3 cut(s) 233, 1230, 1266
BspT107I GGYRCC 1 cut(s) 263
BsrDI GCAATG 2 cut(s) 151, 1146
BsrI ACTGG 2 cut(s) 651, 1186
BssECI CCNNGG 5 cut(s) 259, 563, 1218, 1224, 1311
BssMI GATC 5 cut(s) 389, 497, 964, 1184, 1198
BssT1I CCWWGG 3 cut(s) 563, 1218, 1224
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 157, 1159
Bst6I CTCTTC 4 cut(s) 233, 1041, 1230, 1266
BstC8I GCNNGC 3 cut(s) 213, 693, 953
BstDEI CTNAG 9 cut(s) 174, 618, 702, 756, 770, 834, 956, 978, 1053
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 5 cut(s) 85, 416, 559, 771, 1009
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 5 cut(s) 392, 500, 967, 1187, 1201
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 5 cut(s) 389, 497, 964, 1184, 1198
BstMWI GCNNNNNNNGC 3 cut(s) 122, 263, 1282
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 2 cut(s) 665, 1297
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 5 cut(s) 39, 543, 909, 1167, 1305
BstSLI GKGCMC 2 cut(s) 999, 1164
BstV1I GCAGC 5 cut(s) 53, 337, 471, 1123, 1220
BstV2I GAAGAC 2 cut(s) 186, 1064
BstX2I RGATCY 2 cut(s) 497, 1184
BstYI RGATCY 2 cut(s) 497, 1184
Bsu36I CCTNAGG 1 cut(s) 834
BsuRI GGCC 4 cut(s) 125, 257, 997, 1223
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 5 cut(s) 85, 416, 559, 771, 1009
BtsI GCAGTG 2 cut(s) 1083, 1164
BtsIMutI CAGTG 2 cut(s) 1083, 1164
Cac8I GCNNGC 3 cut(s) 213, 693, 953
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 4 cut(s) 9, 256, 995, 996
Csp6I GTAC 1 cut(s) 573
CviAII CATG 2 cut(s) 662, 1294
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 9 cut(s) 174, 618, 702, 756, 770, 834, 956, 978, 1053
DpnI GATC 5 cut(s) 391, 499, 966, 1186, 1200
DpnII GATC 5 cut(s) 389, 497, 964, 1184, 1198
Eam1104I CTCTTC 4 cut(s) 233, 1041, 1230, 1266
EarI CTCTTC 4 cut(s) 233, 1041, 1230, 1266
Eco130I CCWWGG 3 cut(s) 563, 1218, 1224
Eco24I GRGCYC 1 cut(s) 999
Eco47I GGWCC 1 cut(s) 9
Eco57I CTGAAG 4 cut(s) 500, 534, 866, 939
Eco81I CCTNAGG 1 cut(s) 834
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoO109I RGGNCCY 4 cut(s) 9, 256, 995, 996
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 3 cut(s) 563, 1218, 1224
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 3 cut(s) 563, 1218, 1224
FaeI CATG 2 cut(s) 665, 1297
FaiI YATR 8 cut(s) 29, 225, 588, 639, 663, 1248, 1295, 1307
FalI AAGNNNNNCTT 2 cut(s) 1112, 1144
FaqI GGGAC 3 cut(s) 22, 959, 1228
FatI CATG 2 cut(s) 661, 1293
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 5 cut(s) 42, 351, 460, 1137, 1209
FokI GGATG 5 cut(s) 92, 403, 546, 778, 996
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 5 cut(s) 42, 351, 460, 1137, 1209
FspBI CTAG 4 cut(s) 564, 749, 948, 1041
GlaI GCGC 1 cut(s) 316
GluI GCNGC 5 cut(s) 42, 351, 460, 1137, 1209
GsuI CTGGAG 1 cut(s) 668
HaeIII GGCC 4 cut(s) 125, 257, 997, 1223
HhaI GCGC 1 cut(s) 317
Hin1II CATG 2 cut(s) 665, 1297
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 327, 808
HinfI GANTC 3 cut(s) 505, 521, 1044
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 133, 575, 1111
Hpy188I TCNGA 9 cut(s) 454, 582, 742, 887, 919, 969, 981, 1054, 1315
Hpy188III TCNNGA 5 cut(s) 749, 772, 818, 962, 1202
Hpy8I GTNNAC 3 cut(s) 133, 575, 1111
HpyAV CCTTC 6 cut(s) 22, 247, 463, 509, 542, 1096
HpyCH4III ACNGT 2 cut(s) 157, 1159
HpyCH4IV ACGT 1 cut(s) 889
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 263, 1282
HpyF3I CTNAG 9 cut(s) 174, 618, 702, 756, 770, 834, 956, 978, 1053
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 2 cut(s) 665, 1297
HspAI GCGC 1 cut(s) 315
Kzo9I GATC 5 cut(s) 389, 497, 964, 1184, 1198
LguI GCTCTTC 3 cut(s) 233, 1230, 1266
LmnI GCTCC 4 cut(s) 358, 820, 841, 1315
Lsp1109I GCAGC 5 cut(s) 53, 337, 471, 1123, 1220
LweI GCATC 5 cut(s) 53, 282, 430, 756, 1018
MaeI CTAG 4 cut(s) 564, 749, 948, 1041
MaeII ACGT 1 cut(s) 889
MaeIII GTNAC 1 cut(s) 88
MalI GATC 5 cut(s) 391, 499, 966, 1186, 1200
MboI GATC 5 cut(s) 389, 497, 964, 1184, 1198
MflI RGATCY 2 cut(s) 497, 1184
MhlI GDGCHC 2 cut(s) 999, 1164
MluCI AATT 6 cut(s) 57, 282, 358, 446, 685, 1131
MlyI GAGTC 1 cut(s) 1038
MmeI TCCRAC 1 cut(s) 57
MseI TTAA 3 cut(s) 396, 536, 1130
MslI CAYNNNNRTG 2 cut(s) 1251, 1292
MspA1I CMGCKG 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 122, 263, 1282
NdeII GATC 5 cut(s) 389, 497, 964, 1184, 1198
NlaIII CATG 2 cut(s) 665, 1297
NlaIV GGNNCC 6 cut(s) 10, 258, 265, 499, 822, 997
NspI RCATGY 2 cut(s) 665, 1297
PciSI GCTCTTC 3 cut(s) 233, 1230, 1266
PcsI WCGNNNNNNNCGW 1 cut(s) 1263
PctI GAATGC 1 cut(s) 1156
PfeI GAWTC 2 cut(s) 505, 521
PkrI GCNGC 5 cut(s) 43, 352, 461, 1138, 1210
PleI GAGTC 1 cut(s) 1038
PpsI GAGTC 1 cut(s) 1038
PpuMI RGGWCCY 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 6 cut(s) 10, 258, 265, 499, 822, 997
PspOMI GGGCCC 1 cut(s) 995
PspPI GGNCC 4 cut(s) 9, 256, 995, 996
PspPPI RGGWCCY 1 cut(s) 9
PstI CTGCAG 3 cut(s) 43, 913, 1171
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 2 cut(s) 497, 1184
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
RseI CAYNNNNRTG 2 cut(s) 1251, 1292
SapI GCTCTTC 3 cut(s) 233, 1230, 1266
SaqAI TTAA 3 cut(s) 396, 536, 1130
SatI GCNGC 5 cut(s) 42, 351, 460, 1137, 1209
Sau3AI GATC 5 cut(s) 389, 497, 964, 1184, 1198
Sau96I GGNCC 4 cut(s) 9, 256, 995, 996
SchI GAGTC 1 cut(s) 1038
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 2 cut(s) 999, 1164
SfaNI GCATC 5 cut(s) 53, 282, 430, 756, 1018
SfcI CTRYAG 5 cut(s) 39, 543, 909, 1167, 1305
SinI GGWCC 1 cut(s) 9
SmiMI CAYNNNNRTG 2 cut(s) 1251, 1292
Sse9I AATT 6 cut(s) 57, 282, 358, 446, 685, 1131
SsiI CCGC 1 cut(s) 213
SspMI CTAG 4 cut(s) 564, 749, 948, 1041
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 3 cut(s) 563, 1218, 1224
TaaI ACNGT 2 cut(s) 157, 1159
TaiI ACGT 1 cut(s) 892
TaqI TCGA 1 cut(s) 287
TaqII GACCGA 1 cut(s) 47
TasI AATT 6 cut(s) 57, 282, 358, 446, 685, 1131
TfiI GAWTC 2 cut(s) 505, 521
Tru1I TTAA 3 cut(s) 396, 536, 1130
Tru9I TTAA 3 cut(s) 396, 536, 1130
TscAI CASTG 2 cut(s) 1083, 1171
TseI GCWGC 5 cut(s) 41, 350, 459, 1136, 1208
TspDTI ATGAA 6 cut(s) 17, 305, 624, 1098, 1157, 1284
TspGWI ACGGA 1 cut(s) 1042
TspRI CASTG 2 cut(s) 1083, 1171
VpaK11BI GGWCC 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 748
XceI RCATGY 2 cut(s) 665, 1297
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmaJI CCTAGG 1 cut(s) 563
XspI CTAG 4 cut(s) 564, 749, 948, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.