RLG00000011547

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004715
Physical Location & Seq
Reverse (-)
10437093 .. 10441175
4083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011547

Sequence Viewer

Length: 1440 bp
ATGGCAAATGGCAAATATACGGTGAGGAGTGATCCTCCCTTTTTTCGGGTTCGGATCCTCCGATCCGCCTCCGCCGTCGCCACTGTCTCGCCGCGACTCTCAGATCCTTCAAGGCATGGACTTCTGACTGCCGCACGCTTAACGAGTTTCGTGTGTGTCATGGATTCGGATAATTTGGAAGCCGCCTATGGGCTTCAGGTTGCTCTTGAAAATGGTGGTCATGGGCAACTTAGTGTTGGTCCAGACGGCATTAATGGGAGTGCCGGTGAAACTACTCTTACCGAGACTGCAGCACCGAATGGGAGAATTGAAAATGTGGTGAACTCGGATGATGGTGTGTCTTATAATTCATCTGCTGGAGAAGTCAAAGAGGAATCCAGAGTCAACTCTGCAAGCAATGGTTTGACTATTGCTAAGGAAAGGGGACCGAAAGTTTCTGTTCAGTCAAAGCAATCCAAAGTGCTGAAGGGTCAAGGTAAGAGTAAGATTGAGAAGCCCCCTAGTCCCAAGAGCGCCTTGCCAATTCGGATGAAGAAAAGCAAAGATGGAAATGATGCAGAGGCTACAGCTACTGTTTCCAATGGTACAGCAGGTCCAAATTCACTCGCCAAGCAGCCTAACAAGAGTAGATCATTGAATGGCCAACAGGTTCAGTTATCCAATCAGCAACCTAAACAATCTGAGGCACCATCGACCGAAGGCCTTGTGGAGGAGACAAATCTGAAGCCACTAAACAAAGGCTCCTATAATAAGGCTGATGGAGACTCCCAATCTTCTTTAAGTCCCACAGAAGGAGATATGAAAGCACCTAGGGTGGGTACACTGCCAAAGTATGGTTTCAGCTTTAAGTGTGATGAAAGAGCTGAGAAAAGAAGAGAGTTCTACACCAAACTCGAGGAAAAGATACATGCAAAGGAGATGGAGAAGAATAATTTGCAAGCCAAGTCTAAGTTACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGCCGCAGGAAGAGCTTACCTCCAGCAGATTCTGAAGGAAACAGTAATTCCAAGAGTCAATCTGGCAGGCTTAGTCTGGGTGAGAAACTTCCTCAAAACTCTGCCAAAGGACCTTCTCCTGTGCATCCGAAGAAGCCACAAAGAAAGTCTCTTCCTAGACTTCCATCTGAGAAGACCACTTTATCTAATGTGAAGAGTGTGCGGAAGGTTACTTCTAAGGCAACAAATGAGGAAAAGGCTGCCGGGTTGCCCAATGCAGCAAGTGAAGCTGGACCTCACACCCAAGAGCAGGAGGCAGTTCCCAGAGCTGAGATAACTGAACCCTACACCCAAGAGCCGGAAGCAGTTCCCAGGGCTGCAATAAGTGAGGAGCAGTCCCATTCAGATGATGAGACAGTTAATGAAGAGGAACATCATCCCACCTTGGTTCAAGAGCCTATAGCGCTGGAGAATTGA

Protein Analysis

480

Amino Acids

51.35

Weight (kDa)

9.25

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 280 - 318 8.7e-11 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 685
AccB7I CCANNNNNTGG 1 cut(s) 833
AccII CGCG 1 cut(s) 94
AciI CCGC 7 cut(s) 66, 72, 92, 132, 183, 988, 1186
AclWI GGATC 5 cut(s) 26, 49, 57, 62, 98
AcoI YGGCCR 2 cut(s) 640, 985
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 4 cut(s) 179, 485, 743, 1038
AfaI GTAC 2 cut(s) 586, 820
AfeI AGCGCT 1 cut(s) 1428
AfiI CCNNNNNNNGG 9 cut(s) 45, 189, 709, 791, 815, 833, 983, 1273, 1321
AgsI TTSAA 5 cut(s) 111, 209, 311, 637, 1415
AjnI CCWGG 1 cut(s) 1334
AluBI AGCT 7 cut(s) 569, 843, 863, 982, 999, 1253, 1292
AluI AGCT 7 cut(s) 569, 843, 863, 982, 999, 1253, 1292
Alw26I GTCTC 7 cut(s) 91, 278, 707, 756, 978, 1137, 1370
AlwI GGATC 5 cut(s) 26, 49, 57, 62, 98
AlwNI CAGNNNCTG 2 cut(s) 572, 1016
Ama87I CYCGRG 1 cut(s) 893
Aor51HI AGCGCT 1 cut(s) 1428
AoxI GGCC 3 cut(s) 640, 700, 985
ApeKI GCWGC 5 cut(s) 290, 613, 1223, 1241, 1340
ApoI RAATTY 1 cut(s) 598
AseI ATTAAT 1 cut(s) 252
Asp700I GAANNNNTTC 1 cut(s) 1329
AspA2I CCTAGG 1 cut(s) 809
AspLEI GCGC 2 cut(s) 515, 1429
AspS9I GGNCC 5 cut(s) 239, 425, 593, 1094, 1256
AsuC2I CCSGG 1 cut(s) 1228
AsuHPI GGTGA 4 cut(s) 34, 278, 331, 1076
AvaI CYCGRG 1 cut(s) 893
AvaII GGWCC 5 cut(s) 239, 425, 593, 1094, 1256
AvrII CCTAGG 1 cut(s) 809
BalI TGGCCA 1 cut(s) 642
BamHI GGATCC 1 cut(s) 54
BanI GGYRCC 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 1163
BbvI GCAGC 5 cut(s) 302, 625, 1210, 1253, 1327
BccI CCATC 6 cut(s) 326, 539, 697, 752, 913, 1156
BceAI ACGGC 2 cut(s) 59, 262
BciT130I CCWGG 1 cut(s) 1336
BcnI CCSGG 1 cut(s) 1228
BcoDI GTCTC 7 cut(s) 91, 278, 707, 756, 978, 1137, 1370
BfaI CTAG 3 cut(s) 501, 810, 1140
BfmI CTRYAG 3 cut(s) 288, 564, 1422
BfoI RGCGCY 2 cut(s) 516, 1430
BfuAI ACCTGC 1 cut(s) 581
BisI GCNGC 9 cut(s) 92, 132, 183, 291, 614, 988, 1224, 1242, 1341
BlnI CCTAGG 1 cut(s) 809
BlsI GCNGC 9 cut(s) 93, 133, 184, 292, 615, 989, 1225, 1243, 1342
Bme1390I CCNGG 2 cut(s) 1228, 1336
Bme18I GGWCC 5 cut(s) 239, 425, 593, 1094, 1256
BmeT110I CYCGRG 1 cut(s) 893
BmgT120I GGNCC 5 cut(s) 239, 425, 593, 1094, 1256
BmiI GGNNCC 4 cut(s) 56, 426, 687, 742
BmrFI CCNGG 2 cut(s) 1228, 1336
BmsI GCATC 2 cut(s) 544, 1117
BpiI GAAGAC 1 cut(s) 1163
BplI GAGNNNNNCTC 4 cut(s) 19, 51, 988, 1020
BpmI CTGGAG 2 cut(s) 378, 990
Bpu10I CCTNAGC 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 1228
BsaJI CCNNGG 4 cut(s) 809, 1334, 1335, 1407
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 725, 755
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 189, 709, 791, 815, 833, 983, 1273, 1321
Bse118I RCCGGY 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 403
BseBI CCWGG 1 cut(s) 1336
BseDI CCNNGG 4 cut(s) 809, 1334, 1335, 1407
BseGI GGATG 4 cut(s) 334, 534, 1108, 1399
BseLI CCNNNNNNNGG 9 cut(s) 45, 189, 709, 791, 815, 833, 983, 1273, 1321
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 5 cut(s) 114, 672, 855, 1143, 1284
BseRI GAGGAG 3 cut(s) 40, 725, 1367
BseX3I CGGCCG 1 cut(s) 985
BseXI GCAGC 5 cut(s) 302, 625, 1210, 1253, 1327
Bsh1236I CGCG 1 cut(s) 94
Bsh1285I CGRYCG 2 cut(s) 696, 988
BshFI GGCC 3 cut(s) 642, 702, 987
BshNI GGYRCC 1 cut(s) 685
BsiEI CGRYCG 2 cut(s) 696, 988
BsiHKCI CYCGRG 1 cut(s) 893
BsiSI CCGG 3 cut(s) 264, 1227, 1322
BslFI GGGAC 4 cut(s) 438, 489, 768, 1345
BslI CCNNNNNNNGG 9 cut(s) 45, 189, 709, 791, 815, 833, 983, 1273, 1321
BsmAI GTCTC 7 cut(s) 91, 278, 707, 756, 978, 1137, 1370
BsmFI GGGAC 4 cut(s) 438, 489, 768, 1345
BsnI GGCC 3 cut(s) 642, 702, 987
BsoBI CYCGRG 1 cut(s) 893
Bsp143I GATC 5 cut(s) 31, 54, 62, 103, 629
BspACI CCGC 7 cut(s) 66, 72, 92, 132, 183, 988, 1186
BspANI GGCC 3 cut(s) 642, 702, 987
BspCNI CTCAG 5 cut(s) 113, 673, 856, 1144, 1285
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 4 cut(s) 56, 426, 687, 742
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 5 cut(s) 26, 49, 57, 62, 98
BspQI GCTCTTC 1 cut(s) 989
BspT107I GGYRCC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 403
BsrFI RCCGGY 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 4 cut(s) 809, 1334, 1335, 1407
BssMI GATC 5 cut(s) 31, 54, 62, 103, 629
BssT1I CCWWGG 2 cut(s) 809, 1407
Bst2UI CCWGG 1 cut(s) 1336
Bst4CI ACNGT 5 cut(s) 22, 85, 574, 1028, 1381
Bst6I CTCTTC 5 cut(s) 868, 989, 1140, 1172, 1383
BstC8I GCNNGC 4 cut(s) 136, 394, 939, 1052
BstENI CCTNNNNNAGG 1 cut(s) 707
BstF5I GGATG 4 cut(s) 334, 534, 1108, 1399
BstFNI CGCG 1 cut(s) 94
BstH2I RGCGCY 2 cut(s) 516, 1430
BstHHI GCGC 2 cut(s) 515, 1429
BstKTI GATC 5 cut(s) 34, 57, 65, 106, 632
BstMAI GTCTC 7 cut(s) 91, 278, 707, 756, 978, 1137, 1370
BstMBI GATC 5 cut(s) 31, 54, 62, 103, 629
BstMCI CGRYCG 2 cut(s) 696, 988
BstMWI GCNNNNNNNGC 3 cut(s) 996, 1250, 1426
BstNI CCWGG 1 cut(s) 1336
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 2 cut(s) 1226, 1334
BstSFI CTRYAG 3 cut(s) 288, 564, 1422
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 5 cut(s) 302, 625, 1210, 1253, 1327
BstV2I GAAGAC 1 cut(s) 1163
BstX2I RGATCY 2 cut(s) 54, 103
BstYI RGATCY 2 cut(s) 54, 103
BstZI CGGCCG 1 cut(s) 985
BsuRI GGCC 3 cut(s) 642, 702, 987
BtsCI GGATG 4 cut(s) 334, 534, 1108, 1399
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 2 cut(s) 81, 821
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 4 cut(s) 136, 394, 939, 1052
CaiI CAGNNNCTG 2 cut(s) 572, 1016
CfoI GCGC 2 cut(s) 515, 1429
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 5 cut(s) 239, 425, 593, 1094, 1256
Csp6I GTAC 2 cut(s) 585, 819
CviAII CATG 4 cut(s) 116, 160, 221, 908
CviQI GTAC 2 cut(s) 585, 819
DpnI GATC 5 cut(s) 33, 56, 64, 105, 631
DpnII GATC 5 cut(s) 31, 54, 62, 103, 629
EaeI YGGCCR 2 cut(s) 640, 985
EagI CGGCCG 1 cut(s) 985
Eam1104I CTCTTC 5 cut(s) 868, 989, 1140, 1172, 1383
EarI CTCTTC 5 cut(s) 868, 989, 1140, 1172, 1383
EciI GGCGGA 2 cut(s) 55, 61
EclXI CGGCCG 1 cut(s) 985
Eco130I CCWWGG 2 cut(s) 809, 1407
Eco147I AGGCCT 1 cut(s) 702
Eco47I GGWCC 5 cut(s) 239, 425, 593, 1094, 1256
Eco47III AGCGCT 1 cut(s) 1428
Eco52I CGGCCG 1 cut(s) 985
Eco57I CTGAAG 4 cut(s) 179, 485, 743, 1038
Eco88I CYCGRG 1 cut(s) 893
EcoNI CCTNNNNNAGG 1 cut(s) 707
EcoO109I RGGNCCY 1 cut(s) 1094
EcoRII CCWGG 1 cut(s) 1334
EcoT14I CCWWGG 2 cut(s) 809, 1407
ErhI CCWWGG 2 cut(s) 809, 1407
FaeI CATG 4 cut(s) 119, 163, 224, 911
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 4 cut(s) 438, 489, 768, 1345
FatI CATG 4 cut(s) 115, 159, 220, 907
Fnu4HI GCNGC 9 cut(s) 92, 132, 183, 291, 614, 988, 1224, 1242, 1341
FokI GGATG 4 cut(s) 341, 541, 1095, 1386
Fsp4HI GCNGC 9 cut(s) 92, 132, 183, 291, 614, 988, 1224, 1242, 1341
FspBI CTAG 3 cut(s) 501, 810, 1140
GlaI GCGC 2 cut(s) 514, 1428
GluI GCNGC 9 cut(s) 92, 132, 183, 291, 614, 988, 1224, 1242, 1341
GsuI CTGGAG 2 cut(s) 378, 990
HaeII RGCGCY 2 cut(s) 516, 1430
HaeIII GGCC 3 cut(s) 642, 702, 987
HapII CCGG 3 cut(s) 264, 1227, 1322
HhaI GCGC 2 cut(s) 515, 1429
Hin1II CATG 4 cut(s) 119, 163, 224, 911
Hin6I GCGC 2 cut(s) 513, 1427
HinP1I GCGC 2 cut(s) 513, 1427
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 7 cut(s) 96, 164, 374, 381, 764, 1013, 1039
HpaII CCGG 3 cut(s) 264, 1227, 1322
HphI GGTGA 4 cut(s) 34, 278, 331, 1076
Hpy166II GTNNAC 3 cut(s) 322, 385, 821
Hpy188III TCNNGA 4 cut(s) 206, 242, 378, 1415
Hpy8I GTNNAC 3 cut(s) 322, 385, 821
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 7 cut(s) 117, 460, 692, 785, 1013, 1107, 1183
HpyCH4III ACNGT 5 cut(s) 22, 85, 574, 1028, 1381
HpyCH4V TGCA 8 cut(s) 290, 392, 557, 911, 937, 1108, 1241, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 996, 1250, 1426
Hsp92II CATG 4 cut(s) 119, 163, 224, 911
HspAI GCGC 2 cut(s) 513, 1427
Kzo9I GATC 5 cut(s) 31, 54, 62, 103, 629
LguI GCTCTTC 1 cut(s) 989
LmnI GCTCC 2 cut(s) 746, 1354
Lsp1109I GCAGC 5 cut(s) 302, 625, 1210, 1253, 1327
LweI GCATC 2 cut(s) 544, 1117
MaeI CTAG 3 cut(s) 501, 810, 1140
MaeIII GTNAC 2 cut(s) 951, 1192
MalI GATC 5 cut(s) 33, 56, 64, 105, 631
MboI GATC 5 cut(s) 31, 54, 62, 103, 629
MflI RGATCY 2 cut(s) 54, 103
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 8 cut(s) 172, 306, 346, 522, 598, 931, 1030, 1435
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 4 cut(s) 90, 390, 758, 1048
Mox20I TGGCCA 1 cut(s) 642
MroXI GAANNNNTTC 1 cut(s) 1329
MscI TGGCCA 1 cut(s) 642
MseI TTAA 5 cut(s) 140, 252, 779, 846, 1383
MslI CAYNNNNRTG 1 cut(s) 1368
Msp20I TGGCCA 1 cut(s) 642
MspI CCGG 3 cut(s) 264, 1227, 1322
MspR9I CCNGG 2 cut(s) 1228, 1336
MvaI CCWGG 1 cut(s) 1336
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 3 cut(s) 996, 1250, 1426
NciI CCSGG 1 cut(s) 1228
NdeII GATC 5 cut(s) 31, 54, 62, 103, 629
NlaIII CATG 4 cut(s) 119, 163, 224, 911
NlaIV GGNNCC 4 cut(s) 56, 426, 687, 742
NmeAIII GCCGAG 1 cut(s) 963
NspI RCATGY 1 cut(s) 911
PaeR7I CTCGAG 1 cut(s) 893
PasI CCCWGGG 1 cut(s) 1335
PceI AGGCCT 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 989
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 1329
PfeI GAWTC 3 cut(s) 164, 374, 1013
PflMI CCANNNNNTGG 1 cut(s) 833
PkrI GCNGC 9 cut(s) 93, 133, 184, 292, 615, 989, 1225, 1243, 1342
PleI GAGTC 4 cut(s) 90, 389, 758, 1047
PpsI GAGTC 4 cut(s) 90, 389, 758, 1047
PpuMI RGGWCCY 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 252
PsiI TTATAA 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 1094
Psp6I CCWGG 1 cut(s) 1334
PspGI CCWGG 1 cut(s) 1334
PspN4I GGNNCC 4 cut(s) 56, 426, 687, 742
PspPI GGNCC 5 cut(s) 239, 425, 593, 1094, 1256
PspPPI RGGWCCY 1 cut(s) 1094
PspXI VCTCGAGB 1 cut(s) 893
PstI CTGCAG 1 cut(s) 292
PstNI CAGNNNCTG 2 cut(s) 572, 1016
PsuI RGATCY 2 cut(s) 54, 103
RsaI GTAC 2 cut(s) 586, 820
RsaNI GTAC 2 cut(s) 585, 819
RseI CAYNNNNRTG 1 cut(s) 1368
SapI GCTCTTC 1 cut(s) 989
SaqAI TTAA 5 cut(s) 140, 252, 779, 846, 1383
SatI GCNGC 9 cut(s) 92, 132, 183, 291, 614, 988, 1224, 1242, 1341
Sau3AI GATC 5 cut(s) 31, 54, 62, 103, 629
Sau96I GGNCC 5 cut(s) 239, 425, 593, 1094, 1256
SchI GAGTC 4 cut(s) 90, 390, 758, 1048
ScrFI CCNGG 2 cut(s) 1228, 1336
SfaNI GCATC 2 cut(s) 544, 1117
SfcI CTRYAG 3 cut(s) 288, 564, 1422
Sfr274I CTCGAG 1 cut(s) 893
SinI GGWCC 5 cut(s) 239, 425, 593, 1094, 1256
SlaI CTCGAG 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 1368
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
Sse9I AATT 8 cut(s) 172, 306, 346, 522, 598, 931, 1030, 1435
SseBI AGGCCT 1 cut(s) 702
SsiI CCGC 7 cut(s) 66, 72, 92, 132, 183, 988, 1186
SspMI CTAG 3 cut(s) 501, 810, 1140
StuI AGGCCT 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 1226, 1334
StyI CCWWGG 2 cut(s) 809, 1407
TaaI ACNGT 5 cut(s) 22, 85, 574, 1028, 1381
TaqI TCGA 2 cut(s) 692, 894
TaqII GACCGA 2 cut(s) 442, 710
TasI AATT 8 cut(s) 172, 306, 346, 522, 598, 931, 1030, 1435
TauI GCSGC 4 cut(s) 94, 134, 185, 990
TfiI GAWTC 3 cut(s) 164, 374, 1013
Tru1I TTAA 5 cut(s) 140, 252, 779, 846, 1383
Tru9I TTAA 5 cut(s) 140, 252, 779, 846, 1383
TscAI CASTG 2 cut(s) 88, 828
TseI GCWGC 5 cut(s) 290, 613, 1223, 1241, 1340
TspDTI ATGAA 5 cut(s) 339, 545, 815, 870, 1401
TspRI CASTG 2 cut(s) 88, 828
Van91I CCANNNNNTGG 1 cut(s) 833
VpaK11BI GGWCC 5 cut(s) 239, 425, 593, 1094, 1256
VspI ATTAAT 1 cut(s) 252
XagI CCTNNNNNAGG 1 cut(s) 707
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 893
XmaJI CCTAGG 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 1329
XspI CTAG 3 cut(s) 501, 810, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.