Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006745

Physical Location & Seq

Genomic Coordinates Forward (+)

25697605 .. 25699921

Gene Structure 2317 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1198800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCGTATATTTCTCTCTCTAAACCCTATCGATGGCGGCCTCGACCCTCCAATCTTCAGGCCCTCACGCACCTCCGCCTCTTCAGCTCAACCGAATCCGCCACCGATACCTCTGCTGCCGCAACCGAAGCCGTAGCCGCCACCGAAGCTGCAGCACCAACCGAAGCCGCATCCCCAACTCAAATCCATGTCCCAAAACCTAAGCAGTACCGTCCTCGAAACCCAGAGAAGACAGAGGACATAATCTGCAGAATGATGGCGAATCGGGCCTGGACGACCCGGTTACAGAACTCGATCCGGAACTTGGTACCCGAATTCGACCACAACCTAGTGTGGAACGTCTTGCACGGCGCTCGGAACTGGGAGCACGCCCTCCAATTCTTCAGGTGGGTCGAGCGGTCCGGGTTTTTCAAGCACGACCGGGAGACCCATTTGAAGATTATTGAGATTCTGGGCCGGAATTTGAAGCTCAACCATGCCCGGTGTATCCTTCTGGACATGCCGAAAAAAGGCGAGCAGCTCGATGAGGACCTCTTCCTTGCGCTCATTGATGGCTACGGCAAGGCCGGATACGGTAAGGCTGGCATTATTCAAGAGTCTGTTAAATTGTTTGATAGTATGAAGGAATTGGGTGTGCAGAGAAGTCTCAAATCATATGAGGCTCTCTTTAAGGCCATTATGCGCCGTGGCCGGTATATGATGGCCAAGAGGTACTTTAATGCAATGCTGAGTGAGGGAATAGAGCCAAATAGGCATACTTATAATGTTATGATTTGGGGTTTCTTCATGAGCAAGAGATTAGAGACCGCCAAGCGGTTTTACGAGGATATGAAAAGTAGAGGAATTTTGCCTGATCTCGTTACTTATAACACTATGATTCATGGGTACAATCGGTTTAAGATGATGGATGAGGCAGAGCAGTTGTTTGTGGAGTTGAAGGGGAGGAATTTAGAACCTAATGTGATATGCTATACTACCATGATTAAAGGGTATGTTGATGTTGGGAGAGTTGATGATGCATTGAGGTTGTTTCAAGAGATGAAGTCTTTTGGTATTAAACCGAATGCTGTCACATTTTCGACTCTATTGCCGGGGCTTTGTGAGGCAGAGAAAAAGAATGAAGCTGTGAACATGTTGAAGGAGATGGTTCAGAGGTATATTGCTCCCAAGGATAATTCAATCTTTGAGAAATTGCTATATTTGATGTGCAAGTCTGGGGATTTGGATGCAGCTGCTGATGTCCTCAAGGCAATGATTCGGCTGAGCATTCCCACTGAGCCTGGTCATTATGGTATCCTGATTGAGAACTTTTGCAAGGCCGGGGTTTATGATCGAGCAATCAAGCTGCTGGATAAGCTTATTGAGAAAGAAATTATTTTGAGGCCCCAGAGTTCTATCGAGTTGGAGGCTAGTGCTTATAACCCAATGATTGAGTATTTGTGTAACCATGGGCAGACTGAGAAAGCTGAAGTTTTTTTCCGGCAGTTGATGAAAAAGGGTGTTCAAGATTCAGTTGCCTTCAATAATTTGATGTGCGGACATGCGAAAGAAGGAAATTCTGATTCTGCTTTTGAGATTTTAAGGATCATGGGTAGGAGAGGGGTCCCTGGAGAAGCAGATTCTTACAGGTTACTCATTAACAGCTACTTGAGTAAAGGTGAACCGGCTGATGCTAAAACAGCTTTGGACAGTATGCTTGAAGGCGGGCATATTCCAGAGGCATCACTGTTCGGGTCAGTGATGGAGAGTCTATTTGAAGACGGGAGGGTTCAAACTGCAAGCCGAGTAATGAAAAGTATGGTAGAGAAGGGGGTGAAGGAGAACATGGATTTGGTTGCCAAGATTTTGGAAACCCTCTTTATGAGAGGTCATGTTGAGGAAGCACTGGGACGAATCGATCTACTTATGCAAAGCGGGTGTACGCCACAGTTTGATAGCCTTTTATCTGTTCTTGCTGAGAAAGGGAAAACAATTGCAGCCCTCAAGCTGTTAGATTTTTGCCTTGAGAGAGATTGCAATGTGGATTTCTCAAGCTATGACAAGGTGTTGGATGCTCTCTTAGAAGCAGGGAAGACTCTTAATGCATACTCAATATTGTGTAAAATAATGGAGAAAGGAGGGCTCTCCGACTGGAGTAGCACCAAGGATTTGATTAAGAGCCTAAATCAAGAGGGTAACACGAAACAAGCAGATATCCTCTCCAGAATGATAAAGGGAGGGGATAAAAGTGGTCAGAGCAAAAAAGGAAACAAAGAAGCTGTTCTTGCGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0055035 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

758

Amino Acids

85.49

Weight (kDa)

8.55

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	198 - 336	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	202 - 258	1.2e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	218 - 261	5.6e-09	PPR repeat family
PPR_2	PF13041	253 - 296	2.8e-14	PPR repeat family
PPR_3	PF13812	274 - 332	1.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	280 - 311	7.5e-09	PPR repeat
PPR_2	PF13041	284 - 332	9.4e-15	PPR repeat family
PPR	PF01535	287 - 316	2.7e-07	PPR repeat
PPR_3	PF13812	308 - 363	1.5e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	315 - 348	2.6e-10	PPR repeat
PPR_2	PF13041	319 - 367	1.9e-18	PPR repeat family
PPR	PF01535	323 - 352	2.2e-08	PPR repeat
PPR_long	PF17177	337 - 456	6.1e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	351 - 383	3.6e-08	PPR repeat
PPR_2	PF13041	503 - 549	2.1e-07	PPR repeat family
TPR_24	PF23276	515 - 630	1.5e-06	Fungal tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37230
fragaria_vesca	FvH4_5g34800
malus_domestica	MD08G1198800.v1.1 MD15G1385700.v1.1
prunus_persica	Prupe.1G532500_v2.0.a1
pyrus_communis	pycom08g17270 pycom15g34600
rosa_chinensis	RchiOBHm_Chr6g0274921
rosa_laevigata	RLG00000013509
rosa_multiflora	Rmu_co8266267.1_g000001 Rmu_sc0002311.1_g000019
rosa_roxburghii	Rroxscaffold_1G00028490 Rroxscaffold_7G00193820
rosa_rugosa	Rorug05G0270600 Rorug06G0086200
rosa_samantha	Rh5AG343600 Rh5BG354200 Rh5CG380200 Rh5DG369700 Rh6AG201000 Rh6BG204600 Rh6CG206000 Rh6DG196200
rosa_wichuraiana	Rw5G032350 Rw5G032460 Rw6G017490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	762, 867, 1419
Acc65I	GGTACC	1 cut(s)	307
AccB1I	GGYRCC	1 cut(s)	307
AccBSI	CCGCTC	1 cut(s)	397
AccIII	TCCGGA	1 cut(s)	297
AclWI	GGATC	2 cut(s)	289, 1592
AcoI	YGGCCR	2 cut(s)	688, 702
AcsI	RAATTY	5 cut(s)	314, 460, 843, 946, 1555
AcuI	CTGAAG	4 cut(s)	41, 67, 367, 1488
AfaI	GTAC	5 cut(s)	209, 309, 713, 887, 1921
AfiI	CCNNNNNNNGG	4 cut(s)	33, 304, 509, 813
AflIII	ACRYGT	1 cut(s)	1131
AjnI	CCWGG	3 cut(s)	269, 1279, 1606
AjuI	GAANNNNNNNTTGG	2 cut(s)	1814, 1846
Alw21I	GWGCWC	1 cut(s)	369
Alw26I	GTCTC	3 cut(s)	419, 650, 797
AlwI	GGATC	2 cut(s)	289, 1592
AlwNI	CAGNNNCTG	1 cut(s)	1235
Aor13HI	TCCGGA	1 cut(s)	297
ApeKI	GCWGC	8 cut(s)	116, 149, 152, 517, 1229, 1232, 1345, 1976
ApoI	RAATTY	5 cut(s)	314, 460, 843, 946, 1555
Asp700I	GAANNNNTTC	1 cut(s)	2259
Asp718I	GGTACC	1 cut(s)	307
AspLEI	GCGC	3 cut(s)	353, 544, 684
AspS9I	GGNCC	7 cut(s)	61, 267, 399, 454, 529, 1383, 1603
AsuC2I	CCSGG	6 cut(s)	280, 403, 422, 481, 1092, 1321
AsuHPI	GGTGA	2 cut(s)	1670, 1825
AvaII	GGWCC	3 cut(s)	399, 529, 1603
BalI	TGGCCA	1 cut(s)	704
BanI	GGYRCC	1 cut(s)	307
BanII	GRGCYC	1 cut(s)	2124
BbsI	GAAGAC	3 cut(s)	236, 1764, 2078
Bbv12I	GWGCWC	1 cut(s)	369
BbvI	GCAGC	8 cut(s)	103, 136, 164, 529, 1219, 1241, 1332, 1988
BccI	CCATC	7 cut(s)	27, 250, 545, 694, 898, 1138, 1735
BceAI	ACGGC	4 cut(s)	116, 364, 574, 669
BcgI	CGANNNNNNTGC	6 cut(s)	95, 129, 335, 369, 1069, 1103
BciT130I	CCWGG	3 cut(s)	271, 1281, 1608
BciVI	GTATCC	3 cut(s)	497, 563, 1304
BcnI	CCSGG	6 cut(s)	280, 403, 422, 481, 1092, 1321
BcoDI	GTCTC	3 cut(s)	419, 650, 797
BfaI	CTAG	2 cut(s)	329, 1410
BfmI	CTRYAG	2 cut(s)	150, 247
BfoI	RGCGCY	1 cut(s)	354
BfuI	GTATCC	3 cut(s)	497, 563, 1304
BglI	GCCNNNNNGGC	1 cut(s)	751
BlpI	GCTNAGC	1 cut(s)	1262
Bme1390I	CCNGG	9 cut(s)	271, 280, 403, 422, 481, 1092, 1281, 1321, 1608
Bme18I	GGWCC	3 cut(s)	399, 529, 1603
BmgT120I	GGNCC	7 cut(s)	61, 267, 399, 454, 529, 1383, 1603
BmiI	GGNNCC	4 cut(s)	309, 1385, 1604, 1605
BmrFI	CCNGG	9 cut(s)	271, 280, 403, 422, 481, 1092, 1281, 1321, 1608
BmrI	ACTGGG	2 cut(s)	370, 1895
BmsI	GCATC	6 cut(s)	179, 1006, 1216, 1660, 1730, 2041
BmuI	ACTGGG	2 cut(s)	370, 1895
BpiI	GAAGAC	3 cut(s)	236, 1764, 2078
BpmI	CTGGAG	3 cut(s)	1629, 2152, 2185
Bpu10I	CCTNAGC	1 cut(s)	201
Bpu1102I	GCTNAGC	1 cut(s)	1262
BpuEI	CTTGAG	5 cut(s)	1229, 1669, 1967, 2014, 2024
BpuMI	CCSGG	6 cut(s)	280, 403, 422, 481, 1092, 1321
Bsa29I	ATCGAT	2 cut(s)	31, 1896
BsaI	GGTCTC	2 cut(s)	419, 797
BsaJI	CCNNGG	7 cut(s)	685, 1091, 1167, 1320, 1447, 1606, 2142
BsaWI	WCCGGW	1 cut(s)	297
Bsc4I	CCNNNNNNNGG	4 cut(s)	33, 304, 509, 813
Bse118I	RCCGGY	2 cut(s)	690, 1663
Bse1I	ACTGG	3 cut(s)	365, 1890, 2135
Bse3DI	GCAATG	3 cut(s)	729, 1257, 2023
BseAI	TCCGGA	1 cut(s)	297
BseBI	CCWGG	3 cut(s)	271, 1281, 1608
BseCI	ATCGAT	2 cut(s)	31, 1896
BseDI	CCNNGG	7 cut(s)	685, 1091, 1167, 1320, 1447, 1606, 2142
BseGI	GGATG	4 cut(s)	170, 913, 1231, 2056
BseLI	CCNNNNNNNGG	4 cut(s)	33, 304, 509, 813
BseMI	GCAATG	3 cut(s)	729, 1257, 2023
BseMII	CTCAG	5 cut(s)	719, 1253, 1266, 1449, 1947
BseNI	ACTGG	3 cut(s)	365, 1890, 2135
BseXI	GCAGC	8 cut(s)	103, 136, 164, 529, 1219, 1241, 1332, 1988
BsgI	GTGCAG	1 cut(s)	656
Bsh1285I	CGRYCG	1 cut(s)	421
BshNI	GGYRCC	1 cut(s)	307
BshVI	ATCGAT	2 cut(s)	31, 1896
BsiEI	CGRYCG	1 cut(s)	421
BsiHKAI	GWGCWC	1 cut(s)	369
BslFI	GGGAC	3 cut(s)	176, 1589, 1902
BslI	CCNNNNNNNGG	4 cut(s)	33, 304, 509, 813
BsmAI	GTCTC	3 cut(s)	419, 650, 797
BsmFI	GGGAC	3 cut(s)	176, 1589, 1902
BsmI	GAATGC	2 cut(s)	1069, 1266
Bso31I	GGTCTC	2 cut(s)	419, 797
Bsp1286I	GDGCHC	2 cut(s)	369, 2124
Bsp13I	TCCGGA	1 cut(s)	297
Bsp143I	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
Bsp1720I	GCTNAGC	1 cut(s)	1262
Bsp19I	CCATGG	1 cut(s)	1447
BspCNI	CTCAG	5 cut(s)	720, 1254, 1267, 1450, 1948
BspDI	ATCGAT	2 cut(s)	31, 1896
BspEI	TCCGGA	1 cut(s)	297
BspHI	TCATGA	1 cut(s)	786
BspLI	GGNNCC	4 cut(s)	309, 1385, 1604, 1605
BspMAI	CTGCAG	2 cut(s)	154, 251
BspPI	GGATC	2 cut(s)	289, 1592
BspT107I	GGYRCC	1 cut(s)	307
BspTNI	GGTCTC	2 cut(s)	419, 797
BsrBI	CCGCTC	1 cut(s)	397
BsrDI	GCAATG	3 cut(s)	729, 1257, 2023
BsrFI	RCCGGY	2 cut(s)	690, 1663
BsrI	ACTGG	3 cut(s)	365, 1890, 2135
BssAI	RCCGGY	2 cut(s)	690, 1663
BssECI	CCNNGG	7 cut(s)	685, 1091, 1167, 1320, 1447, 1606, 2142
BssMI	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
BssT1I	CCWWGG	3 cut(s)	1167, 1447, 2142
Bst2UI	CCWGG	3 cut(s)	271, 1281, 1608
Bst4CI	ACNGT	5 cut(s)	212, 575, 1691, 1728, 1929
Bst6I	CTCTTC	2 cut(s)	86, 539
BstC8I	GCNNGC	5 cut(s)	369, 515, 583, 1706, 1780
BstDEI	CTNAG	7 cut(s)	201, 728, 1262, 1275, 1458, 1956, 2059
BstDSI	CCRYGG	2 cut(s)	685, 1447
BstF5I	GGATG	4 cut(s)	170, 913, 1231, 2056
BstH2I	RGCGCY	1 cut(s)	354
BstHHI	GCGC	3 cut(s)	353, 544, 684
BstKTI	GATC	5 cut(s)	297, 856, 1333, 1587, 1900
BstMAI	GTCTC	3 cut(s)	419, 650, 797
BstMBI	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
BstMCI	CGRYCG	1 cut(s)	421
BstMWI	GCNNNNNNNGC	9 cut(s)	84, 128, 137, 146, 266, 751, 1354, 1679, 2264
BstNI	CCWGG	3 cut(s)	271, 1281, 1608
BstNSI	RCATGY	3 cut(s)	502, 1135, 1544
BstSCI	CCNGG	9 cut(s)	269, 278, 401, 420, 479, 1090, 1279, 1319, 1606
BstSFI	CTRYAG	2 cut(s)	150, 247
BstV1I	GCAGC	8 cut(s)	103, 136, 164, 529, 1219, 1241, 1332, 1988
BstV2I	GAAGAC	3 cut(s)	236, 1764, 2078
BstXI	CCANNNNNNTGG	1 cut(s)	1846
Bsu15I	ATCGAT	2 cut(s)	31, 1896
BsuI	GTATCC	3 cut(s)	497, 563, 1304
BsuTUI	ATCGAT	2 cut(s)	31, 1896
BtgI	CCRYGG	2 cut(s)	685, 1447
BtsCI	GGATG	4 cut(s)	170, 913, 1231, 2056
BtsIMutI	CAGTG	4 cut(s)	1272, 1724, 1743, 1883
Cac8I	GCNNGC	5 cut(s)	369, 515, 583, 1706, 1780
CaiI	CAGNNNCTG	1 cut(s)	1235
CciI	TCATGA	1 cut(s)	786
CfoI	GCGC	3 cut(s)	353, 544, 684
Cfr10I	RCCGGY	2 cut(s)	690, 1663
Cfr13I	GGNCC	7 cut(s)	61, 267, 399, 454, 529, 1383, 1603
ClaI	ATCGAT	2 cut(s)	31, 1896
CpoI	CGGWCCG	1 cut(s)	399
Csp6I	GTAC	5 cut(s)	208, 308, 712, 886, 1920
CspI	CGGWCCG	1 cut(s)	399
CviQI	GTAC	5 cut(s)	208, 308, 712, 886, 1920
DdeI	CTNAG	7 cut(s)	201, 728, 1262, 1275, 1458, 1956, 2059
DpnI	GATC	5 cut(s)	296, 855, 1332, 1586, 1899
DpnII	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
EaeI	YGGCCR	2 cut(s)	688, 702
Eam1104I	CTCTTC	2 cut(s)	86, 539
EarI	CTCTTC	2 cut(s)	86, 539
EciI	GGCGGA	2 cut(s)	65, 88
Eco130I	CCWWGG	3 cut(s)	1167, 1447, 2142
Eco24I	GRGCYC	1 cut(s)	2124
Eco31I	GGTCTC	2 cut(s)	419, 797
Eco32I	GATATC	1 cut(s)	2194
Eco47I	GGWCC	3 cut(s)	399, 529, 1603
Eco57I	CTGAAG	4 cut(s)	41, 67, 367, 1488
EcoO109I	RGGNCCY	4 cut(s)	61, 529, 1383, 1603
EcoRI	GAATTC	1 cut(s)	314
EcoRII	CCWGG	3 cut(s)	269, 1279, 1606
EcoRV	GATATC	1 cut(s)	2194
EcoT14I	CCWWGG	3 cut(s)	1167, 1447, 2142
EcoT22I	ATGCAT	2 cut(s)	1021, 2086
EcoT38I	GRGCYC	1 cut(s)	2124
ErhI	CCWWGG	3 cut(s)	1167, 1447, 2142
FalI	AAGNNNNNCTT	3 cut(s)	698, 730, 2247
FaqI	GGGAC	3 cut(s)	176, 1589, 1902
FauI	CCCGC	2 cut(s)	1697, 1907
FauNDI	CATATG	1 cut(s)	655
FokI	GGATG	4 cut(s)	157, 920, 1238, 2063
FriOI	GRGCYC	1 cut(s)	2124
FspBI	CTAG	2 cut(s)	329, 1410
GlaI	GCGC	3 cut(s)	352, 543, 683
GsuI	CTGGAG	3 cut(s)	1629, 2152, 2185
HaeII	RGCGCY	1 cut(s)	354
HhaI	GCGC	3 cut(s)	353, 544, 684
Hin6I	GCGC	3 cut(s)	351, 542, 682
HinP1I	GCGC	3 cut(s)	351, 542, 682
HindIII	AAGCTT	1 cut(s)	1355
HphI	GGTGA	2 cut(s)	1670, 1825
Hpy166II	GTNNAC	3 cut(s)	1129, 1661, 1920
Hpy188I	TCNGA	5 cut(s)	357, 1152, 1561, 2128, 2235
Hpy8I	GTNNAC	3 cut(s)	1129, 1661, 1920
HpyAV	CCTTC	9 cut(s)	500, 616, 931, 1132, 1528, 1544, 1694, 1801, 1810
HpyCH4III	ACNGT	5 cut(s)	212, 575, 1691, 1728, 1929
HpyCH4IV	ACGT	1 cut(s)	339
HpyF10VI	GCNNNNNNNGC	9 cut(s)	84, 128, 137, 146, 266, 751, 1354, 1679, 2264
HpyF3I	CTNAG	7 cut(s)	201, 728, 1262, 1275, 1458, 1956, 2059
HpySE526I	ACGT	1 cut(s)	339
HspAI	GCGC	3 cut(s)	351, 542, 682
KflI	GGGWCCC	1 cut(s)	1603
Kpn2I	TCCGGA	1 cut(s)	297
KpnI	GGTACC	1 cut(s)	311
Kzo9I	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
LmnI	GCTCC	2 cut(s)	364, 1168
Lsp1109I	GCAGC	8 cut(s)	103, 136, 164, 529, 1219, 1241, 1332, 1988
LweI	GCATC	6 cut(s)	179, 1006, 1216, 1660, 1730, 2041
MaeI	CTAG	2 cut(s)	329, 1410
MaeII	ACGT	1 cut(s)	339
MaeIII	GTNAC	6 cut(s)	282, 859, 1069, 1442, 1629, 2174
MalI	GATC	5 cut(s)	296, 855, 1332, 1586, 1899
MbiI	CCGCTC	1 cut(s)	397
MboI	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
MboII	GAAGA	9 cut(s)	47, 73, 241, 373, 448, 526, 775, 1769, 2083
MfeI	CAATTG	1 cut(s)	1971
MhlI	GDGCHC	2 cut(s)	369, 2124
MlsI	TGGCCA	1 cut(s)	704
MluNI	TGGCCA	1 cut(s)	704
MlyI	GAGTC	4 cut(s)	605, 1075, 1756, 2068
MmeI	TCCRAC	3 cut(s)	1383, 2028, 2151
Mox20I	TGGCCA	1 cut(s)	704
Mph1103I	ATGCAT	2 cut(s)	1021, 2086
MroI	TCCGGA	1 cut(s)	297
MroXI	GAANNNNTTC	1 cut(s)	2259
MscI	TGGCCA	1 cut(s)	704
Msp20I	TGGCCA	1 cut(s)	704
MspA1I	CMGCKG	1 cut(s)	1232
MspR9I	CCNGG	9 cut(s)	271, 280, 403, 422, 481, 1092, 1281, 1321, 1608
MunI	CAATTG	1 cut(s)	1971
Mva1269I	GAATGC	2 cut(s)	1069, 1266
MvaI	CCWGG	3 cut(s)	271, 1281, 1608
MwoI	GCNNNNNNNGC	9 cut(s)	84, 128, 137, 146, 266, 751, 1354, 1679, 2264
NciI	CCSGG	6 cut(s)	280, 403, 422, 481, 1092, 1321
NcoI	CCATGG	1 cut(s)	1447
NdeI	CATATG	1 cut(s)	655
NdeII	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
NlaIV	GGNNCC	4 cut(s)	309, 1385, 1604, 1605
NmeAIII	GCCGAG	1 cut(s)	1808
NmuCI	GTSAC	1 cut(s)	1069
NsiI	ATGCAT	2 cut(s)	1021, 2086
NspI	RCATGY	3 cut(s)	502, 1135, 1544
PagI	TCATGA	1 cut(s)	786
PciI	ACATGT	1 cut(s)	1131
PctI	GAATGC	2 cut(s)	1069, 1266
PdmI	GAANNNNTTC	1 cut(s)	2259
PfeI	GAWTC	9 cut(s)	95, 262, 448, 877, 1255, 1508, 1562, 1619, 1893
PleI	GAGTC	4 cut(s)	604, 1075, 1755, 2068
PpsI	GAGTC	4 cut(s)	604, 1075, 1755, 2068
PpuMI	RGGWCCY	2 cut(s)	529, 1603
PscI	ACATGT	1 cut(s)	1131
PsiI	TTATAA	3 cut(s)	762, 867, 1419
Psp5II	RGGWCCY	2 cut(s)	529, 1603
Psp6I	CCWGG	3 cut(s)	269, 1279, 1606
PspGI	CCWGG	3 cut(s)	269, 1279, 1606
PspN4I	GGNNCC	4 cut(s)	309, 1385, 1604, 1605
PspPI	GGNCC	7 cut(s)	61, 267, 399, 454, 529, 1383, 1603
PspPPI	RGGWCCY	2 cut(s)	529, 1603
PstI	CTGCAG	2 cut(s)	154, 251
PstNI	CAGNNNCTG	1 cut(s)	1235
PvuII	CAGCTG	1 cut(s)	1232
RsaI	GTAC	5 cut(s)	209, 309, 713, 887, 1921
RsaNI	GTAC	5 cut(s)	208, 308, 712, 886, 1920
Rsr2I	CGGWCCG	1 cut(s)	399
RsrII	CGGWCCG	1 cut(s)	399
Sau3AI	GATC	5 cut(s)	294, 853, 1330, 1584, 1897
Sau96I	GGNCC	7 cut(s)	61, 267, 399, 454, 529, 1383, 1603
SchI	GAGTC	4 cut(s)	605, 1075, 1756, 2068
ScrFI	CCNGG	9 cut(s)	271, 280, 403, 422, 481, 1092, 1281, 1321, 1608
SduI	GDGCHC	2 cut(s)	369, 2124
SfaNI	GCATC	6 cut(s)	179, 1006, 1216, 1660, 1730, 2041
SfcI	CTRYAG	2 cut(s)	150, 247
SinI	GGWCC	3 cut(s)	399, 529, 1603
SmlI	CTYRAG	5 cut(s)	1244, 1648, 1982, 2003, 2029
SmoI	CTYRAG	5 cut(s)	1244, 1648, 1982, 2003, 2029
SspI	AATATT	1 cut(s)	2094
SspMI	CTAG	2 cut(s)	329, 1410
StyD4I	CCNGG	9 cut(s)	269, 278, 401, 420, 479, 1090, 1279, 1319, 1606
StyI	CCWWGG	3 cut(s)	1167, 1447, 2142
TaaI	ACNGT	5 cut(s)	212, 575, 1691, 1728, 1929
TaiI	ACGT	1 cut(s)	342
TauI	GCSGC	4 cut(s)	40, 122, 140, 170
TfiI	GAWTC	9 cut(s)	95, 262, 448, 877, 1255, 1508, 1562, 1619, 1893
TscAI	CASTG	4 cut(s)	1279, 1731, 1743, 1890
TseFI	GTSAC	1 cut(s)	1069
TseI	GCWGC	8 cut(s)	116, 149, 152, 517, 1229, 1232, 1345, 1976
Tsp45I	GTSAC	1 cut(s)	1069
TspDTI	ATGAA	8 cut(s)	635, 775, 845, 869, 1055, 1134, 1505, 1805
TspRI	CASTG	4 cut(s)	1279, 1731, 1743, 1890
VpaK11BI	GGWCC	3 cut(s)	399, 529, 1603
XapI	RAATTY	5 cut(s)	314, 460, 843, 946, 1555
XceI	RCATGY	3 cut(s)	502, 1135, 1544
XmnI	GAANNNNTTC	1 cut(s)	2259
XspI	CTAG	2 cut(s)	329, 1410
Zsp2I	ATGCAT	2 cut(s)	1021, 2086

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.