Rroxscaffold_1G00028490

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006745
Physical Location & Seq
Reverse (-)
36283258 .. 36284899
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028490.1

Sequence Viewer

Length: 1218 bp
ATGCCCGAGGACATCATCTGTAGAATGATGGCCAATCGGGCCTGGACGACCCGGTTGCAGAACTCCATCCGCGATTTGGTACCCGAATTCGATCACAATCTCGTCTGGAATGTCCTTCACGCAGCTAACACCTCCGACCAAGCCCTCCAATTCTTCCGATGGGTCGAGAGTTCCAGGTTGTTTCAGCATGATAGGGGCACCCATTTGAAGCTTATTGAGATTCTGGGCCGAGCTTCGAAGCTCAACCATGCCCGGTGTATCCTACTGGATATGCCCAAGAAGGGTGTGCAATGGGATGAGGACCTCTTCATTCATCTTATTGATAGTTATGCAAAGGCCGGCATTGTTCAGGAGTCGGTCAAGCTTTTCAATCAGATGAAGGAATTGGACTGCAAAACGGTTAATCCTGATGTTGTCACCTATAACACGATGAGTAATGGGTACAATTGGTTTAAGCTGATGGATGAGGCCGAGCAGTTGTTTGTGGAGTTGAAGGGAAAGAATATACAGCCCAATGTTATTAGATATACTACCATGATTAAAGGGTATGTTTCTGTTGGGAAAGTGGATGATGGATTCAGGCTGTTTCAAGAGATGAAGTCTTTTGGTATTAAGCCTAATGATGTCACTGTAAGTCTGGGGACTTGGGATGCAGCTGCCAATGTGCTCAAGGCAATGATTAGGCTGCACATTCCGACAGAAGCTGGTCATTATGGTATCCTTATTGAGAACTTTTGCAAGGCTGGGGTGTGTGATCGAGCAGTTCACTTGCTTGATAAGCTCATTGAGAAAGAAATTATTATGAGGTCCCAGAGTTCTATGGAATTGGAGGCTAGTGCTTACAACCCAATGATTGAGTATCTGTGCAACCATGGGCAGACAGGTAAAGCTGAAGTCCTTTTCAGGCAGTTGATGAAAAAGGGAGTTCAGGATTCAGTTGCCTTCAATAATTTGATCCGTGGGCATGCTAAGGAAGGAAATTCTGATTCTGCTTTTGAGATTTTAAAGATTATGGGTAGAAGAGGGGTTCCTAGAGAAGCAGATTCCTACAAGTTGCTCATTAAGAGCTACCTAAGTAAAGGTGAACCAGCTGATGCTAAAACAGCTTTGGACAGTATGATTGAAAATGGGCATGTTCCAGAATCATCACTATTCAGGTCAGTAATGGAGAGTCTATTTGAAGATGGTAGGGTTCAAACGGCAAGCCGCGTAATGTAG

Protein Analysis

405

Amino Acids

46.03

Weight (kDa)

6.87

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 105 - 148 3.3e-08 PPR repeat family
PPR_2 PF13041 136 - 183 8.6e-11 PPR repeat family
PPR_3 PF13812 160 - 210 1.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 167 - 200 4.5e-08 PPR repeat
PPR_2 PF13041 171 - 212 3.7e-12 PPR repeat family
PPR PF01535 176 - 204 1.8e-07 PPR repeat
PPR_2 PF13041 311 - 355 1.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 79, 197
AccII CGCG 2 cut(s) 72, 1209
AciI CCGC 2 cut(s) 70, 1207
AclWI GGATC 1 cut(s) 949
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 2 cut(s) 86, 979
AcuI CTGAAG 1 cut(s) 912
AfaI GTAC 2 cut(s) 81, 443
AfiI CCNNNNNNNGG 2 cut(s) 76, 281
AgsI TTSAA 8 cut(s) 208, 370, 493, 590, 946, 1124, 1181, 1196
AjnI CCWGG 2 cut(s) 41, 173
Alw21I GWGCWC 1 cut(s) 669
AlwI GGATC 1 cut(s) 949
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 5
AoxI GGCC 5 cut(s) 30, 39, 226, 336, 468
ApeKI GCWGC 4 cut(s) 122, 653, 656, 685
ApoI RAATTY 2 cut(s) 86, 979
Asp718I GGTACC 1 cut(s) 79
AspS9I GGNCC 4 cut(s) 39, 226, 301, 807
AsuC2I CCSGG 2 cut(s) 52, 253
AsuHPI GGTGA 2 cut(s) 409, 1094
AsuII TTCGAA 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 5
AvaII GGWCC 2 cut(s) 301, 807
BaeGI GKGCMC 1 cut(s) 200
BalI TGGCCA 1 cut(s) 32
BanI GGYRCC 2 cut(s) 79, 197
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 4 cut(s) 134, 643, 665, 672
BccI CCATC 6 cut(s) 22, 74, 153, 454, 566, 1178
BceAI ACGGC 1 cut(s) 1215
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BciT130I CCWGG 2 cut(s) 43, 175
BciVI GTATCC 2 cut(s) 269, 728
BcnI CCSGG 2 cut(s) 52, 253
BfaI CTAG 2 cut(s) 834, 1032
BfmI CTRYAG 1 cut(s) 19
BfuI GTATCC 2 cut(s) 269, 728
BglI GCCNNNNNGGC 1 cut(s) 38
BisI GCNGC 5 cut(s) 123, 654, 657, 686, 1207
BlsI GCNGC 5 cut(s) 124, 655, 658, 687, 1208
Bme1390I CCNGG 4 cut(s) 43, 52, 175, 253
Bme18I GGWCC 2 cut(s) 301, 807
BmeT110I CYCGRG 1 cut(s) 5
BmgT120I GGNCC 4 cut(s) 39, 226, 301, 807
BmiI GGNNCC 4 cut(s) 81, 199, 809, 1029
BmrFI CCNGG 4 cut(s) 43, 52, 175, 253
BmsI GCATC 2 cut(s) 640, 1084
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 52, 253
BsaBI GATNNNNATC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 6, 871, 958
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 281
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 2 cut(s) 296, 681
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 2 cut(s) 43, 175
BseDI CCNNGG 3 cut(s) 6, 871, 958
BseGI GGATG 5 cut(s) 66, 301, 469, 574, 655
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 2 cut(s) 76, 281
BseMI GCAATG 2 cut(s) 296, 681
BseNI ACTGG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 4 cut(s) 134, 643, 665, 672
BseYI CCCAGC 1 cut(s) 743
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 2 cut(s) 72, 1209
BshFI GGCC 5 cut(s) 32, 41, 228, 338, 470
BshNI GGYRCC 2 cut(s) 79, 197
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 5
BsiSI CCGG 3 cut(s) 52, 253, 339
BslFI GGGAC 2 cut(s) 655, 793
BslI CCNNNNNNNGG 2 cut(s) 76, 281
BsmFI GGGAC 2 cut(s) 655, 793
BsnI GGCC 5 cut(s) 32, 41, 228, 338, 470
BsoBI CYCGRG 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 200, 669
Bsp143I GATC 3 cut(s) 91, 754, 954
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 2 cut(s) 70, 1207
BspANI GGCC 5 cut(s) 32, 41, 228, 338, 470
BspFNI CGCG 2 cut(s) 72, 1209
BspLI GGNNCC 4 cut(s) 81, 199, 809, 1029
BspPI GGATC 1 cut(s) 949
BspT104I TTCGAA 1 cut(s) 236
BspT107I GGYRCC 2 cut(s) 79, 197
BsrDI GCAATG 2 cut(s) 296, 681
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 1 cut(s) 270
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 6, 871, 958
BssMI GATC 3 cut(s) 91, 754, 954
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 43, 175
Bst4CI ACNGT 3 cut(s) 400, 631, 1115
Bst6I CTCTTC 2 cut(s) 311, 1015
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 340, 966, 1204
BstDEI CTNAG 2 cut(s) 969, 1073
BstDSI CCRYGG 2 cut(s) 871, 958
BstF5I GGATG 5 cut(s) 66, 301, 469, 574, 655
BstFNI CGCG 2 cut(s) 72, 1209
BstKTI GATC 3 cut(s) 94, 757, 957
BstMBI GATC 3 cut(s) 91, 754, 954
BstMWI GCNNNNNNNGC 3 cut(s) 38, 778, 1103
BstNI CCWGG 2 cut(s) 43, 175
BstNSI RCATGY 2 cut(s) 968, 1136
BstSCI CCNGG 4 cut(s) 41, 50, 173, 251
BstSFI CTRYAG 1 cut(s) 19
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 2 cut(s) 72, 1209
BstV1I GCAGC 4 cut(s) 134, 643, 665, 672
BsuI GTATCC 2 cut(s) 269, 728
BsuRI GGCC 5 cut(s) 32, 41, 228, 338, 470
BtgI CCRYGG 2 cut(s) 871, 958
BtsCI GGATG 5 cut(s) 66, 301, 469, 574, 655
BtsIMutI CAGTG 1 cut(s) 627
Cac8I GCNNGC 3 cut(s) 340, 966, 1204
CaiI CAGNNNCTG 1 cut(s) 704
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 4 cut(s) 39, 226, 301, 807
Csp6I GTAC 2 cut(s) 80, 442
CviAII CATG 6 cut(s) 188, 248, 535, 872, 965, 1133
CviQI GTAC 2 cut(s) 80, 442
DdeI CTNAG 2 cut(s) 969, 1073
DpnI GATC 3 cut(s) 93, 756, 956
DpnII GATC 3 cut(s) 91, 754, 954
DraI TTTAAA 1 cut(s) 1005
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 311, 1015
EarI CTCTTC 2 cut(s) 311, 1015
Eco130I CCWWGG 1 cut(s) 871
Eco47I GGWCC 2 cut(s) 301, 807
Eco57I CTGAAG 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 5
EcoO109I RGGNCCY 2 cut(s) 301, 807
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 41, 173
EcoT14I CCWWGG 1 cut(s) 871
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 6 cut(s) 191, 251, 538, 875, 968, 1136
FaqI GGGAC 2 cut(s) 655, 793
FatI CATG 6 cut(s) 187, 247, 534, 871, 964, 1132
Fnu4HI GCNGC 5 cut(s) 123, 654, 657, 686, 1207
FokI GGATG 5 cut(s) 53, 308, 476, 581, 662
Fsp4HI GCNGC 5 cut(s) 123, 654, 657, 686, 1207
FspBI CTAG 2 cut(s) 834, 1032
GluI GCNGC 5 cut(s) 123, 654, 657, 686, 1207
GsaI CCCAGC 1 cut(s) 747
HaeIII GGCC 5 cut(s) 32, 41, 228, 338, 470
HapII CCGG 3 cut(s) 52, 253, 339
Hin1II CATG 6 cut(s) 191, 251, 538, 875, 968, 1136
HindIII AAGCTT 2 cut(s) 209, 362
HinfI GANTC 8 cut(s) 220, 353, 576, 932, 986, 1043, 1142, 1171
HpaII CCGG 3 cut(s) 52, 253, 339
HphI GGTGA 2 cut(s) 409, 1094
Hpy166II GTNNAC 2 cut(s) 766, 1085
Hpy188I TCNGA 5 cut(s) 136, 158, 375, 696, 985
Hpy188III TCNNGA 7 cut(s) 106, 166, 350, 407, 590, 929, 1139
Hpy8I GTNNAC 2 cut(s) 766, 1085
HpyAV CCTTC 6 cut(s) 125, 274, 373, 487, 952, 968
HpyCH4III ACNGT 3 cut(s) 400, 631, 1115
HpyCH4V TGCA 8 cut(s) 58, 289, 332, 393, 653, 688, 738, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 778, 1103
HpyF3I CTNAG 2 cut(s) 969, 1073
Hsp92II CATG 6 cut(s) 191, 251, 538, 875, 968, 1136
KpnI GGTACC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
Kzo9I GATC 3 cut(s) 91, 754, 954
Lsp1109I GCAGC 4 cut(s) 134, 643, 665, 672
LweI GCATC 2 cut(s) 640, 1084
MaeI CTAG 2 cut(s) 834, 1032
MaeIII GTNAC 2 cut(s) 415, 625
MalI GATC 3 cut(s) 93, 756, 956
MboI GATC 3 cut(s) 91, 754, 954
MboII GAAGA 4 cut(s) 145, 298, 1032, 1193
MfeI CAATTG 1 cut(s) 445
MhlI GDGCHC 2 cut(s) 200, 669
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 8 cut(s) 86, 149, 383, 445, 795, 824, 949, 979
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 2 cut(s) 362, 1180
MmeI TCCRAC 2 cut(s) 159, 719
MnlI CCTC 8 cut(s) 142, 155, 292, 314, 460, 798, 823, 1016
Mox20I TGGCCA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 338
MscI TGGCCA 1 cut(s) 32
MseI TTAA 6 cut(s) 402, 453, 540, 612, 1004, 1062
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 2 cut(s) 656, 1091
MspI CCGG 3 cut(s) 52, 253, 339
MspR9I CCNGG 4 cut(s) 43, 52, 175, 253
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 2 cut(s) 43, 175
MvnI CGCG 2 cut(s) 72, 1209
MwoI GCNNNNNNNGC 3 cut(s) 38, 778, 1103
NaeI GCCGGC 1 cut(s) 340
NciI CCSGG 2 cut(s) 52, 253
NcoI CCATGG 1 cut(s) 871
NdeII GATC 3 cut(s) 91, 754, 954
NgoMIV GCCGGC 1 cut(s) 338
NlaIII CATG 6 cut(s) 191, 251, 538, 875, 968, 1136
NlaIV GGNNCC 4 cut(s) 81, 199, 809, 1029
NmeAIII GCCGAG 2 cut(s) 254, 496
NmuCI GTSAC 2 cut(s) 415, 625
NspI RCATGY 2 cut(s) 968, 1136
NspV TTCGAA 1 cut(s) 236
PaeI GCATGC 1 cut(s) 968
PdiI GCCGGC 1 cut(s) 340
PfeI GAWTC 6 cut(s) 220, 576, 932, 986, 1043, 1142
PkrI GCNGC 5 cut(s) 124, 655, 658, 687, 1208
PleI GAGTC 2 cut(s) 361, 1179
PpsI GAGTC 2 cut(s) 361, 1179
PpuMI RGGWCCY 2 cut(s) 301, 807
Psp5II RGGWCCY 2 cut(s) 301, 807
Psp6I CCWGG 2 cut(s) 41, 173
PspFI CCCAGC 1 cut(s) 743
PspGI CCWGG 2 cut(s) 41, 173
PspN4I GGNNCC 4 cut(s) 81, 199, 809, 1029
PspPI GGNCC 4 cut(s) 39, 226, 301, 807
PspPPI RGGWCCY 2 cut(s) 301, 807
PstNI CAGNNNCTG 1 cut(s) 704
PvuII CAGCTG 2 cut(s) 656, 1091
RsaI GTAC 2 cut(s) 81, 443
RsaNI GTAC 2 cut(s) 80, 442
SaqAI TTAA 6 cut(s) 402, 453, 540, 612, 1004, 1062
SatI GCNGC 5 cut(s) 123, 654, 657, 686, 1207
Sau3AI GATC 3 cut(s) 91, 754, 954
Sau96I GGNCC 4 cut(s) 39, 226, 301, 807
SchI GAGTC 2 cut(s) 362, 1180
ScrFI CCNGG 4 cut(s) 43, 52, 175, 253
SduI GDGCHC 2 cut(s) 200, 669
SfaNI GCATC 2 cut(s) 640, 1084
SfcI CTRYAG 1 cut(s) 19
SfiI GGCCNNNNNGGCC 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 236
SinI GGWCC 2 cut(s) 301, 807
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SphI GCATGC 1 cut(s) 968
Sse9I AATT 8 cut(s) 86, 149, 383, 445, 795, 824, 949, 979
SsiI CCGC 2 cut(s) 70, 1207
SspMI CTAG 2 cut(s) 834, 1032
StyD4I CCNGG 4 cut(s) 41, 50, 173, 251
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 400, 631, 1115
TaqI TCGA 4 cut(s) 90, 165, 236, 757
TaqII GACCGA 1 cut(s) 346
TasI AATT 8 cut(s) 86, 149, 383, 445, 795, 824, 949, 979
TauI GCSGC 1 cut(s) 1209
TfiI GAWTC 6 cut(s) 220, 576, 932, 986, 1043, 1142
Tru1I TTAA 6 cut(s) 402, 453, 540, 612, 1004, 1062
Tru9I TTAA 6 cut(s) 402, 453, 540, 612, 1004, 1062
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 2 cut(s) 415, 625
TseI GCWGC 4 cut(s) 122, 653, 656, 685
Tsp45I GTSAC 2 cut(s) 415, 625
TspDTI ATGAA 5 cut(s) 298, 302, 392, 611, 929
TspGWI ACGGA 1 cut(s) 947
TspRI CASTG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 301, 807
XapI RAATTY 2 cut(s) 86, 979
XceI RCATGY 2 cut(s) 968, 1136
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 2 cut(s) 834, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.