Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0006745

Physical Location & Seq

Genomic Coordinates Forward (+)

34394713 .. 34397004

Gene Structure 2292 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g34600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGCCATCAATGGCGCACATTTCTCTCTCAAAACCCTACCGATGGCGACCTCGACCCTACAATCCTCAGTCCCTCACGCTCCTCCGCCTCTTCAGCTCAACCGAATCCCCCACCGGAGCCACAGCGGCAACCGAGTCCGCCACCGAAGCCCCAGCGGCAACCGAAGCTGTAGCCGCCGCCGAAGCCGCAGCCCCAGCCCAGACCCACGTCCCAAAACCTAAGCAGTACCGTCATCGAAACCCAGAGAAGACAGAGGACATAATCTGCAGAATGATGGCGAATCGGGCCTGGACGACCCGGTTGCAGAACTCGATCCGGAACTTGGTACCCGAATTCGACCACAACCTTGTGTGGAATGTCTTGCACGGCGCTCGGAACTGGGAGCACGCCCTCCAATTCTTCAGGTGGGTTGAGCGGTCCGGATTATTCAAACACGACCGCGAGACCCATTTGAAGATTATTGAGATTCTGGGCCGGAATTTGAAGCTCAACCATGCCCGGTGCATCCTTCTGGACATGCCGAAAAAGGGCGTGCAGCTCGACGAGGATCTCTTCCTTGCACTCATTGATGGCTACGGCAAGGCGGGCTACGGTAAGGCTGGCATTATTCAGGAGTCTGTTAAGTTGTTTGATAGTATGAAGGAATTGGGTGTGCAGAGAAGTCTCAAGTCCTATGAGGCTCTCTTTAAGGCCATTATGCGCCGCGGCCGGTATATGATGGCCAAGAGGTATTTTAATGCAATGTTGAGTGAGGGAATAGAGCCCGATAGGCATACTTATAATGTTATGATTTGGGGTTTCTTCATGAGCAAGAGGTTAGAGACCGCCAAGCGGTTTTATGAGGATATGAAGAGTAGAGGAATTTCCCCGGATCTGGTTACTTATAACACTATGATTCATGGGTACAATCGGTTTAAGATGATGGATGAGGCAGAGCAGTTGTTTGTGGAGTTGAAGGGGAGAAATATAGAACCTAATGTGATATGCTATACTACCATGATTAAAGGGTATGTTGATGTTGGGAGAGTTGATGATGCTTTGAGGTTGTTTCAAGAGATGAAGTCTTTTGGTATTAAGCCGAATGCTGTCACGTTTTCAACTCTGTTGCCGGGGCTTTGTGAGGCTGAGAAAAAGGATGAAGCTGTGAACCTGTTGAAGGAGATGGTTCAGAGGTATATTGCTCCCAAGGATAATGCAATCTTTGAGAAATTGCTATCTTTGATGTGCAAATCTGGGGATTTGGATGCAGCTGCTGATGTCCTCAAGGCAATGATTCGGCTGAGCATTCCCACAGAGCCTGGTCATTATGGTATCCTGATTGAGAACTTTTGCAAGGCTGGAGTTTATGATCGAGCAATCAAGCTGCTTGATAAGCTTATTGAGAAAGAAATTATTTTGAGGCCCCAGAGTTCTATCGAGTTGGAGGCTAGTGCTTATAACCCAATGATTGAGCATCTGTGCAACCATGGGCAGACTGAGAAAGCTGAAGTCTTTTTCCGGCAGTTGATGAAAAAGGGTGTTCAGGATTCAGTTGCCTTCAATAATTTGGTGTGTGGGCATGCGAAAGAAGGAAAGTCTGATTCTGCTTTTGAGATTTTAAGGATCATGGGTAGGAGAGGGGTCCCTGGAGAAGCAGATTCTTACAGGTTACTCATTAACAGCTACTTGAGTAAAGGTGAACCGGCTGATGCCAAAACAGCTTTGGACAGTATGCTTGAAAGCGGGCATATTCCAGAGTCACCACTGTTCAGGTCAGTGATAGAGAGTCTATTTGAAGATGGGAGGGTTCAAACTGCAAGCCGAGTAATGAAGAGTATGGTAGAGAAGGGGGTGAAGGAGAACATGGATTTGGTTGCCAAGATTTTGGAAGCCCTCTTTATGAGAGGTCATGTTGAGGAAGCAGTGGGACGAATCGATCTACTTATGCAAAGTGGATGTACACCGCAGTTCGATAGCCTTTTATCTGTTCTTGCTGAGAAAGGGAAAACAATTGCCGCCCTCAAGCTGTTGGATTTTTGCCTCGAGAGGGATTGCAGTGTAGATTTCTCAAGCTATGATAAGGTGTTGGATGCTCTCTTGGAAGCAGGGAAGACTCTTAATGCATACTCAATATTGTGTAAAATAATGGAGAAAGGAGAGGCCTCCGATTGGAGTAGCACCAAAGATTTGATTAAGAGCCTAAATCAAGAGGGTAACACGAAACAAGCAGATATCCTCTCCAGAATGATAAAGGGAGGGGAGAAAAGTGGTCAGAGCAAAAAAGGAAAGAATGAAGCTGTTGTTGCTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0055035 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

764

Amino Acids

85.88

Weight (kDa)

8.56

Isoelectric Point (pI)

41.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	208 - 264	1.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	224 - 267	6.5e-09	PPR repeat family
PPR_2	PF13041	259 - 302	7.8e-15	PPR repeat family
PPR_long	PF17177	273 - 369	8e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	280 - 338	1.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	286 - 317	3.4e-09	PPR repeat
PPR_2	PF13041	290 - 338	3.9e-15	PPR repeat family
PPR	PF01535	293 - 323	1.9e-07	PPR repeat
PPR_long	PF17177	308 - 390	7.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	314 - 369	4.3e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	321 - 354	3e-10	PPR repeat
PPR_2	PF13041	325 - 373	1.9e-18	PPR repeat family
PPR	PF01535	329 - 358	2.2e-08	PPR repeat
PPR_long	PF17177	344 - 462	6.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	357 - 389	5.3e-09	PPR repeat
PPR_2	PF13041	509 - 554	9.9e-07	PPR repeat family
TPR_24	PF23276	521 - 636	7.8e-08	Fungal tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37230
fragaria_vesca	FvH4_5g34800
malus_domestica	MD08G1198800.v1.1 MD15G1385700.v1.1
prunus_persica	Prupe.1G532500_v2.0.a1
pyrus_communis	pycom08g17270 pycom15g34600
rosa_chinensis	RchiOBHm_Chr6g0274921
rosa_laevigata	RLG00000013509
rosa_multiflora	Rmu_co8266267.1_g000001 Rmu_sc0002311.1_g000019
rosa_roxburghii	Rroxscaffold_1G00028490 Rroxscaffold_7G00193820
rosa_rugosa	Rorug05G0270600 Rorug06G0086200
rosa_samantha	Rh5AG343600 Rh5BG354200 Rh5CG380200 Rh5DG369700 Rh6AG201000 Rh6BG204600 Rh6CG206000 Rh6DG196200
rosa_wichuraiana	Rw5G032350 Rw5G032460 Rw6G017490

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	780, 885, 1437
Acc65I	GGTACC	1 cut(s)	325
AccB1I	GGYRCC	1 cut(s)	325
AccBSI	CCGCTC	1 cut(s)	415
AccII	CGCG	2 cut(s)	441, 705
AccIII	TCCGGA	2 cut(s)	315, 419
AclWI	GGATC	4 cut(s)	307, 555, 879, 1610
AcoI	YGGCCR	2 cut(s)	706, 720
AcsI	RAATTY	3 cut(s)	332, 478, 861
AcuI	CTGAAG	3 cut(s)	76, 385, 1506
AfaI	GTAC	4 cut(s)	227, 327, 905, 1939
AfiI	CCNNNNNNNGG	8 cut(s)	42, 322, 527, 831, 874, 1156, 2026, 2148
AjiI	CACGTC	1 cut(s)	208
AjnI	CCWGG	3 cut(s)	287, 1297, 1624
AjuI	GAANNNNNNNTTGG	2 cut(s)	1832, 1864
Alw21I	GWGCWC	1 cut(s)	387
Alw26I	GTCTC	3 cut(s)	437, 668, 815
AlwI	GGATC	4 cut(s)	307, 555, 879, 1610
AlwNI	CAGNNNCTG	2 cut(s)	1253, 1298
Ama87I	CYCGRG	1 cut(s)	2021
Aor13HI	TCCGGA	2 cut(s)	315, 419
AoxI	GGCC	7 cut(s)	285, 472, 690, 706, 720, 1400, 2139
ApeKI	GCWGC	5 cut(s)	188, 535, 1247, 1250, 1363
ApoI	RAATTY	3 cut(s)	332, 478, 861
Asp718I	GGTACC	1 cut(s)	325
AspLEI	GCGC	3 cut(s)	16, 371, 702
AspS9I	GGNCC	5 cut(s)	285, 417, 472, 1401, 1621
AsuC2I	CCSGG	4 cut(s)	298, 499, 869, 1110
AsuHPI	GGTGA	3 cut(s)	1688, 1731, 1843
AvaI	CYCGRG	1 cut(s)	2021
AvaII	GGWCC	2 cut(s)	417, 1621
BalI	TGGCCA	1 cut(s)	722
BanI	GGYRCC	1 cut(s)	325
BanII	GRGCYC	1 cut(s)	765
BbsI	GAAGAC	2 cut(s)	254, 2096
Bbv12I	GWGCWC	1 cut(s)	387
BbvI	GCAGC	5 cut(s)	200, 547, 1237, 1259, 1350
BccI	CCATC	8 cut(s)	13, 36, 268, 563, 712, 916, 1156, 1772
BceAI	ACGGC	2 cut(s)	382, 592
BcgI	CGANNNNNNTGC	4 cut(s)	283, 317, 353, 387
BciT130I	CCWGG	3 cut(s)	289, 1299, 1626
BciVI	GTATCC	1 cut(s)	1322
BcnI	CCSGG	4 cut(s)	298, 499, 869, 1110
BcoDI	GTCTC	3 cut(s)	437, 668, 815
BfaI	CTAG	1 cut(s)	1428
BfmI	CTRYAG	2 cut(s)	168, 265
BfoI	RGCGCY	1 cut(s)	372
BfuI	GTATCC	1 cut(s)	1322
BglI	GCCNNNNNGGC	3 cut(s)	125, 155, 769
BlpI	GCTNAGC	1 cut(s)	1280
Bme1390I	CCNGG	7 cut(s)	289, 298, 499, 869, 1110, 1299, 1626
Bme18I	GGWCC	2 cut(s)	417, 1621
BmeT110I	CYCGRG	1 cut(s)	2021
BmgBI	CACGTC	1 cut(s)	208
BmgT120I	GGNCC	5 cut(s)	285, 417, 472, 1401, 1621
BmiI	GGNNCC	5 cut(s)	118, 327, 1403, 1622, 1623
BmrFI	CCNGG	7 cut(s)	289, 298, 499, 869, 1110, 1299, 1626
BmrI	ACTGGG	1 cut(s)	388
BmsI	GCATC	6 cut(s)	513, 1024, 1234, 1462, 1678, 2059
BmuI	ACTGGG	1 cut(s)	388
BoxI	GACNNNNGTC	1 cut(s)	206
BpiI	GAAGAC	2 cut(s)	254, 2096
BpmI	CTGGAG	3 cut(s)	1359, 1647, 2203
Bpu10I	CCTNAGC	1 cut(s)	219
Bpu1102I	GCTNAGC	1 cut(s)	1280
BpuEI	CTTGAG	5 cut(s)	650, 1247, 1687, 1985, 2032
BpuMI	CCSGG	4 cut(s)	298, 499, 869, 1110
Bsa29I	ATCGAT	1 cut(s)	1914
BsaI	GGTCTC	2 cut(s)	437, 815
BsaJI	CCNNGG	6 cut(s)	703, 867, 1109, 1185, 1465, 1624
BsaWI	WCCGGW	3 cut(s)	113, 315, 419
Bsc4I	CCNNNNNNNGG	8 cut(s)	42, 322, 527, 831, 874, 1156, 2026, 2148
Bse118I	RCCGGY	2 cut(s)	708, 1681
Bse1I	ACTGG	1 cut(s)	383
Bse3DI	GCAATG	2 cut(s)	747, 1275
BseAI	TCCGGA	2 cut(s)	315, 419
BseBI	CCWGG	3 cut(s)	289, 1299, 1626
BseCI	ATCGAT	1 cut(s)	1914
BseDI	CCNNGG	6 cut(s)	703, 867, 1109, 1185, 1465, 1624
BseGI	GGATG	6 cut(s)	504, 931, 1141, 1249, 1940, 2074
BseLI	CCNNNNNNNGG	8 cut(s)	42, 322, 527, 831, 874, 1156, 2026, 2148
BseMI	GCAATG	2 cut(s)	747, 1275
BseMII	CTCAG	5 cut(s)	80, 1116, 1271, 1467, 1965
BseNI	ACTGG	1 cut(s)	383
BseRI	GAGGAG	1 cut(s)	71
BseX3I	CGGCCG	1 cut(s)	706
BseXI	GCAGC	5 cut(s)	200, 547, 1237, 1259, 1350
BseYI	CCCAGC	2 cut(s)	151, 193
BsgI	GTGCAG	2 cut(s)	554, 674
Bsh1236I	CGCG	2 cut(s)	441, 705
Bsh1285I	CGRYCG	2 cut(s)	439, 709
BshFI	GGCC	7 cut(s)	287, 474, 692, 708, 722, 1402, 2141
BshNI	GGYRCC	1 cut(s)	325
BshVI	ATCGAT	1 cut(s)	1914
BsiEI	CGRYCG	2 cut(s)	439, 709
BsiHKAI	GWGCWC	1 cut(s)	387
BsiHKCI	CYCGRG	1 cut(s)	2021
BslFI	GGGAC	4 cut(s)	55, 194, 1607, 1920
BslI	CCNNNNNNNGG	8 cut(s)	42, 322, 527, 831, 874, 1156, 2026, 2148
BsmAI	GTCTC	3 cut(s)	437, 668, 815
BsmFI	GGGAC	4 cut(s)	55, 194, 1607, 1920
BsmI	GAATGC	2 cut(s)	1087, 1284
BsnI	GGCC	7 cut(s)	287, 474, 692, 708, 722, 1402, 2141
Bso31I	GGTCTC	2 cut(s)	437, 815
BsoBI	CYCGRG	1 cut(s)	2021
Bsp1286I	GDGCHC	2 cut(s)	387, 765
Bsp13I	TCCGGA	2 cut(s)	315, 419
Bsp1407I	TGTACA	1 cut(s)	1937
Bsp143I	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
Bsp1720I	GCTNAGC	1 cut(s)	1280
Bsp19I	CCATGG	1 cut(s)	1465
BspANI	GGCC	7 cut(s)	287, 474, 692, 708, 722, 1402, 2141
BspCNI	CTCAG	5 cut(s)	79, 1117, 1272, 1468, 1966
BspDI	ATCGAT	1 cut(s)	1914
BspEI	TCCGGA	2 cut(s)	315, 419
BspFNI	CGCG	2 cut(s)	441, 705
BspHI	TCATGA	1 cut(s)	804
BspLI	GGNNCC	5 cut(s)	118, 327, 1403, 1622, 1623
BspMAI	CTGCAG	1 cut(s)	269
BspPI	GGATC	4 cut(s)	307, 555, 879, 1610
BspT107I	GGYRCC	1 cut(s)	325
BspTNI	GGTCTC	2 cut(s)	437, 815
BsrBI	CCGCTC	1 cut(s)	415
BsrDI	GCAATG	2 cut(s)	747, 1275
BsrFI	RCCGGY	2 cut(s)	708, 1681
BsrGI	TGTACA	1 cut(s)	1937
BsrI	ACTGG	1 cut(s)	383
BssAI	RCCGGY	2 cut(s)	708, 1681
BssECI	CCNNGG	6 cut(s)	703, 867, 1109, 1185, 1465, 1624
BssMI	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
BssT1I	CCWWGG	2 cut(s)	1185, 1465
Bst2UI	CCWGG	3 cut(s)	289, 1299, 1626
Bst4CI	ACNGT	4 cut(s)	230, 593, 1709, 1746
Bst6I	CTCTTC	4 cut(s)	95, 557, 845, 1805
BstAUI	TGTACA	1 cut(s)	1937
BstC8I	GCNNGC	7 cut(s)	387, 533, 586, 601, 1560, 1724, 1798
BstDEI	CTNAG	6 cut(s)	66, 219, 1125, 1280, 1476, 1974
BstDSI	CCRYGG	2 cut(s)	703, 1465
BstENI	CCTNNNNNAGG	1 cut(s)	1154
BstF5I	GGATG	6 cut(s)	504, 931, 1141, 1249, 1940, 2074
BstFNI	CGCG	2 cut(s)	441, 705
BstH2I	RGCGCY	1 cut(s)	372
BstHHI	GCGC	3 cut(s)	16, 371, 702
BstKTI	GATC	6 cut(s)	315, 550, 874, 1351, 1605, 1918
BstMAI	GTCTC	3 cut(s)	437, 668, 815
BstMBI	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
BstMCI	CGRYCG	2 cut(s)	439, 709
BstNI	CCWGG	3 cut(s)	289, 1299, 1626
BstNSI	RCATGY	2 cut(s)	520, 1562
BstPAI	GACNNNNGTC	1 cut(s)	206
BstSCI	CCNGG	7 cut(s)	287, 296, 497, 867, 1108, 1297, 1624
BstSFI	CTRYAG	2 cut(s)	168, 265
BstUI	CGCG	2 cut(s)	441, 705
BstV1I	GCAGC	5 cut(s)	200, 547, 1237, 1259, 1350
BstV2I	GAAGAC	2 cut(s)	254, 2096
BstX2I	RGATCY	2 cut(s)	547, 871
BstXI	CCANNNNNNTGG	1 cut(s)	1864
BstYI	RGATCY	2 cut(s)	547, 871
BstZI	CGGCCG	1 cut(s)	706
Bsu15I	ATCGAT	1 cut(s)	1914
BsuI	GTATCC	1 cut(s)	1322
BsuRI	GGCC	7 cut(s)	287, 474, 692, 708, 722, 1402, 2141
BsuTUI	ATCGAT	1 cut(s)	1914
BtgI	CCRYGG	2 cut(s)	703, 1465
BtrI	CACGTC	1 cut(s)	208
BtsCI	GGATG	6 cut(s)	504, 931, 1141, 1249, 1940, 2074
BtsI	GCAGTG	2 cut(s)	1908, 2041
BtsIMutI	CAGTG	4 cut(s)	1742, 1761, 1908, 2041
Cac8I	GCNNGC	7 cut(s)	387, 533, 586, 601, 1560, 1724, 1798
CaiI	CAGNNNCTG	2 cut(s)	1253, 1298
CciI	TCATGA	1 cut(s)	804
CfoI	GCGC	3 cut(s)	16, 371, 702
Cfr10I	RCCGGY	2 cut(s)	708, 1681
Cfr13I	GGNCC	5 cut(s)	285, 417, 472, 1401, 1621
Cfr42I	CCGCGG	1 cut(s)	706
ClaI	ATCGAT	1 cut(s)	1914
CpoI	CGGWCCG	1 cut(s)	417
Csp6I	GTAC	4 cut(s)	226, 326, 904, 1938
CspI	CGGWCCG	1 cut(s)	417
CviQI	GTAC	4 cut(s)	226, 326, 904, 1938
DdeI	CTNAG	6 cut(s)	66, 219, 1125, 1280, 1476, 1974
DpnI	GATC	6 cut(s)	314, 549, 873, 1350, 1604, 1917
DpnII	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
EaeI	YGGCCR	2 cut(s)	706, 720
EagI	CGGCCG	1 cut(s)	706
Eam1104I	CTCTTC	4 cut(s)	95, 557, 845, 1805
EarI	CTCTTC	4 cut(s)	95, 557, 845, 1805
EciI	GGCGGA	2 cut(s)	74, 127
EclXI	CGGCCG	1 cut(s)	706
Eco130I	CCWWGG	2 cut(s)	1185, 1465
Eco147I	AGGCCT	1 cut(s)	2141
Eco24I	GRGCYC	1 cut(s)	765
Eco31I	GGTCTC	2 cut(s)	437, 815
Eco32I	GATATC	1 cut(s)	2212
Eco47I	GGWCC	2 cut(s)	417, 1621
Eco52I	CGGCCG	1 cut(s)	706
Eco57I	CTGAAG	3 cut(s)	76, 385, 1506
Eco88I	CYCGRG	1 cut(s)	2021
EcoNI	CCTNNNNNAGG	1 cut(s)	1154
EcoO109I	RGGNCCY	2 cut(s)	1401, 1621
EcoRI	GAATTC	1 cut(s)	332
EcoRII	CCWGG	3 cut(s)	287, 1297, 1624
EcoRV	GATATC	1 cut(s)	2212
EcoT14I	CCWWGG	2 cut(s)	1185, 1465
EcoT22I	ATGCAT	1 cut(s)	2104
EcoT38I	GRGCYC	1 cut(s)	765
ErhI	CCWWGG	2 cut(s)	1185, 1465
FaqI	GGGAC	4 cut(s)	55, 194, 1607, 1920
FauI	CCCGC	2 cut(s)	577, 1715
FokI	GGATG	6 cut(s)	491, 938, 1148, 1256, 1947, 2081
FriOI	GRGCYC	1 cut(s)	765
FspBI	CTAG	1 cut(s)	1428
GlaI	GCGC	3 cut(s)	15, 370, 701
GsaI	CCCAGC	2 cut(s)	155, 197
GsuI	CTGGAG	3 cut(s)	1359, 1647, 2203
HaeII	RGCGCY	1 cut(s)	372
HaeIII	GGCC	7 cut(s)	287, 474, 692, 708, 722, 1402, 2141
HhaI	GCGC	3 cut(s)	16, 371, 702
Hin6I	GCGC	3 cut(s)	14, 369, 700
HinP1I	GCGC	3 cut(s)	14, 369, 700
HindIII	AAGCTT	1 cut(s)	1373
HphI	GGTGA	3 cut(s)	1688, 1731, 1843
Hpy166II	GTNNAC	3 cut(s)	1147, 1679, 1940
Hpy188I	TCNGA	5 cut(s)	375, 1170, 1579, 2146, 2253
Hpy8I	GTNNAC	3 cut(s)	1147, 1679, 1940
Hpy99I	CGWCG	1 cut(s)	545
HpyAV	CCTTC	8 cut(s)	518, 634, 949, 1150, 1546, 1562, 1819, 1828
HpyCH4III	ACNGT	4 cut(s)	230, 593, 1709, 1746
HpyCH4IV	ACGT	2 cut(s)	207, 1091
HpyF3I	CTNAG	6 cut(s)	66, 219, 1125, 1280, 1476, 1974
HpySE526I	ACGT	2 cut(s)	207, 1091
HspAI	GCGC	3 cut(s)	14, 369, 700
KflI	GGGWCCC	1 cut(s)	1621
Kpn2I	TCCGGA	2 cut(s)	315, 419
KpnI	GGTACC	1 cut(s)	329
KspI	CCGCGG	1 cut(s)	706
Kzo9I	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
LmnI	GCTCC	4 cut(s)	84, 116, 382, 1186
Lsp1109I	GCAGC	5 cut(s)	200, 547, 1237, 1259, 1350
LweI	GCATC	6 cut(s)	513, 1024, 1234, 1462, 1678, 2059
MaeI	CTAG	1 cut(s)	1428
MaeII	ACGT	2 cut(s)	207, 1091
MaeIII	GTNAC	5 cut(s)	877, 1087, 1647, 1737, 2192
MalI	GATC	6 cut(s)	314, 549, 873, 1350, 1604, 1917
MbiI	CCGCTC	1 cut(s)	415
MboI	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
MfeI	CAATTG	1 cut(s)	1989
MflI	RGATCY	2 cut(s)	547, 871
MhlI	GDGCHC	2 cut(s)	387, 765
MlsI	TGGCCA	1 cut(s)	722
MluCI	AATT	9 cut(s)	332, 395, 478, 644, 861, 1208, 1389, 1543, 1989
MluNI	TGGCCA	1 cut(s)	722
MlyI	GAGTC	5 cut(s)	143, 623, 1745, 1774, 2086
MmeI	TCCRAC	3 cut(s)	1401, 1989, 2046
Mox20I	TGGCCA	1 cut(s)	722
Mph1103I	ATGCAT	1 cut(s)	2104
MroI	TCCGGA	2 cut(s)	315, 419
MscI	TGGCCA	1 cut(s)	722
Msp20I	TGGCCA	1 cut(s)	722
MspA1I	CMGCKG	4 cut(s)	125, 155, 705, 1250
MspR9I	CCNGG	7 cut(s)	289, 298, 499, 869, 1110, 1299, 1626
MunI	CAATTG	1 cut(s)	1989
Mva1269I	GAATGC	2 cut(s)	1087, 1284
MvaI	CCWGG	3 cut(s)	289, 1299, 1626
MvnI	CGCG	2 cut(s)	441, 705
NciI	CCSGG	4 cut(s)	298, 499, 869, 1110
NcoI	CCATGG	1 cut(s)	1465
NdeII	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
NlaIV	GGNNCC	5 cut(s)	118, 327, 1403, 1622, 1623
NmeAIII	GCCGAG	1 cut(s)	1826
NmuCI	GTSAC	2 cut(s)	1087, 1737
NsiI	ATGCAT	1 cut(s)	2104
NspI	RCATGY	2 cut(s)	520, 1562
PaeI	GCATGC	1 cut(s)	1562
PaeR7I	CTCGAG	1 cut(s)	2021
PagI	TCATGA	1 cut(s)	804
PceI	AGGCCT	1 cut(s)	2141
PctI	GAATGC	2 cut(s)	1087, 1284
PfeI	GAWTC	9 cut(s)	104, 280, 466, 895, 1273, 1526, 1580, 1637, 1911
PleI	GAGTC	5 cut(s)	142, 622, 1744, 1773, 2086
PpsI	GAGTC	5 cut(s)	142, 622, 1744, 1773, 2086
PpuMI	RGGWCCY	1 cut(s)	1621
PshAI	GACNNNNGTC	1 cut(s)	206
PsiI	TTATAA	3 cut(s)	780, 885, 1437
Psp5II	RGGWCCY	1 cut(s)	1621
Psp6I	CCWGG	3 cut(s)	287, 1297, 1624
PspFI	CCCAGC	2 cut(s)	151, 193
PspGI	CCWGG	3 cut(s)	287, 1297, 1624
PspN4I	GGNNCC	5 cut(s)	118, 327, 1403, 1622, 1623
PspPI	GGNCC	5 cut(s)	285, 417, 472, 1401, 1621
PspPPI	RGGWCCY	1 cut(s)	1621
PstI	CTGCAG	1 cut(s)	269
PstNI	CAGNNNCTG	2 cut(s)	1253, 1298
PsuI	RGATCY	2 cut(s)	547, 871
PvuII	CAGCTG	1 cut(s)	1250
RsaI	GTAC	4 cut(s)	227, 327, 905, 1939
RsaNI	GTAC	4 cut(s)	226, 326, 904, 1938
Rsr2I	CGGWCCG	1 cut(s)	417
RsrII	CGGWCCG	1 cut(s)	417
SacII	CCGCGG	1 cut(s)	706
Sau3AI	GATC	6 cut(s)	312, 547, 871, 1348, 1602, 1915
Sau96I	GGNCC	5 cut(s)	285, 417, 472, 1401, 1621
SchI	GAGTC	5 cut(s)	143, 623, 1745, 1774, 2086
ScrFI	CCNGG	7 cut(s)	289, 298, 499, 869, 1110, 1299, 1626
SduI	GDGCHC	2 cut(s)	387, 765
SfaNI	GCATC	6 cut(s)	513, 1024, 1234, 1462, 1678, 2059
SfcI	CTRYAG	2 cut(s)	168, 265
Sfr274I	CTCGAG	1 cut(s)	2021
Sfr303I	CCGCGG	1 cut(s)	706
SgrBI	CCGCGG	1 cut(s)	706
SinI	GGWCC	2 cut(s)	417, 1621
SlaI	CTCGAG	1 cut(s)	2021
SmlI	CTYRAG	6 cut(s)	665, 1262, 1666, 2000, 2021, 2047
SmoI	CTYRAG	6 cut(s)	665, 1262, 1666, 2000, 2021, 2047
SphI	GCATGC	1 cut(s)	1562
Sse9I	AATT	9 cut(s)	332, 395, 478, 644, 861, 1208, 1389, 1543, 1989
SseBI	AGGCCT	1 cut(s)	2141
SspI	AATATT	1 cut(s)	2112
SspMI	CTAG	1 cut(s)	1428
StuI	AGGCCT	1 cut(s)	2141
StyD4I	CCNGG	7 cut(s)	287, 296, 497, 867, 1108, 1297, 1624
StyI	CCWWGG	2 cut(s)	1185, 1465
TaaI	ACNGT	4 cut(s)	230, 593, 1709, 1746
TaiI	ACGT	2 cut(s)	210, 1094
TasI	AATT	9 cut(s)	332, 395, 478, 644, 861, 1208, 1389, 1543, 1989
TatI	WGTACW	1 cut(s)	1937
TauI	GCSGC	8 cut(s)	128, 158, 176, 179, 188, 705, 708, 1997
TfiI	GAWTC	9 cut(s)	104, 280, 466, 895, 1273, 1526, 1580, 1637, 1911
TscAI	CASTG	4 cut(s)	1749, 1761, 1908, 2041
TseFI	GTSAC	2 cut(s)	1087, 1737
TseI	GCWGC	5 cut(s)	188, 535, 1247, 1250, 1363
Tsp45I	GTSAC	2 cut(s)	1087, 1737
TspDTI	ATGAA	9 cut(s)	653, 793, 863, 887, 1073, 1152, 1523, 1823, 2286
TspRI	CASTG	4 cut(s)	1749, 1761, 1908, 2041
VpaK11BI	GGWCC	2 cut(s)	417, 1621
XagI	CCTNNNNNAGG	1 cut(s)	1154
XapI	RAATTY	3 cut(s)	332, 478, 861
XceI	RCATGY	2 cut(s)	520, 1562
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1699
XhoI	CTCGAG	1 cut(s)	2021
XspI	CTAG	1 cut(s)	1428
Zsp2I	ATGCAT	1 cut(s)	2104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.