Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0006745

Physical Location & Seq

Genomic Coordinates Forward (+)

49705109 .. 49706560

Gene Structure 1452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG380200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGCCCAAGAAGGGTTTGCAATGGGATGAGGACCTCTTCATTCATCTTATTGATAGTTATGGAAAGGCCGGCATTGTTCAGGAGTCGGTTAAGCTTTTCAATCAGATGAAGGAATTGGGTGTGGAGCGAAGTCTCAAGTCGTACGAGGCTCTGTTTAAGAGCCTTTTGCGGAGGGGGCGGTATATGATGGGGAAGAGGTACTTTAATCACATGTTGGCTGAGGGAATAGAGCCCACTAGGCATACTTATAACATTATGATTTGGGGTTTCTTCTTGTCCTTGAGGTTGGAGACTGCAAAACGGTTTTTCGAGGACATGAAAAGTAGAGGAATTAATCCTGATGTTGTCACCTATAACACGATGATTAATGGGTACAATCGGTTTAAGCTGATGGATGAGGCCGAGCAGTTGTTTGTGGAGTTGAAGGGAAAGAATATACAGCCCAATGTTATAAGTTATACTACCATGATTAAAGGGTATGTTTCTGTTGGGAGAGTGGATGATGGATTCAGGCTGTTTCAAGAGATGAAGTCTTTTGGTATTAAGCCTAATGATGTCACATTTTCGAATTTGTTGCCAGGGCTTTGCGATGCGGAGAAAAAGGATGAAGCTCAGAATATATTGAATGAGATGGTTGAGAGGCATATTGCTCCCAAGGATAATTCAATCTTCGAGAAATTGTTATATTGTCAGTGTAAGTCTGGGGATTTGGATGCGGCTGCCAATGTGCTCAAGGCAATGATTAGGCTGCACGTTCCGACAGAGGCTGGTCATTACGGTATCCTTATTGAGAACTTTTGCAAGGCTGGGGTGTATGATCGAGCAGTTCACTTGCTTGATAAGCTCATTGAGAAAGAAATTATTGTGAGGCCCCAAAGTTCTATGGAATTGGAGGCTAGTGCTTACAACCCAATGATTGAGTATCTGTGCAACCATGGGCAGACAGGTAAAGCTGAAGTCCTTTTCAGGCAGTTGATGAAAAAGGGAGTTCAGGATTCAGTTGCCTTCAATAATTTGATCCGTGGGCATGCTAAGGAAGGAAATTCTGATTCTGCTTTTGAGATTTTAAAGATTATGGGTAGAAGAGGGGTTCCTAGAGAAGCAGATTCCTACAAGTTGCTCATTAAGAGCTACCTAAGTAAAGGTGAACCAGCTGATGCTAAAACAGCTTTGGACAGTATGATTGAAAATGGGCATGATCCAGAATCATCACTATTCAGGTCAGTAATGGAGAGTCTATTTGAAGATGGTAGGGTTCAAACGGCAAGCCGCGTAATGAAGAGTATGGTAGAGAAGGGAGTGAATGAGAACATGGATTTGGTCGCCAAGGTTTTGGAAGCCCTCTTTATCAGAGTTCATGTTGAGGAAGCACTTGGACGGGTTGATCTACTTATGCAAAGTGGTTGTGCGCCTGAGTTTGATAGCCTCTTATCTCTGCTTGCTGAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0055035 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

483

Amino Acids

54.85

Weight (kDa)

6.46

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	14 - 56	6.1e-06	PPR repeat family
PPR_2	PF13041	47 - 90	1.7e-07	PPR repeat family
PPR_2	PF13041	82 - 125	1.6e-14	PPR repeat family
PPR_long	PF17177	96 - 192	4.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	102 - 161	1.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	109 - 140	2.7e-10	PPR repeat
PPR_2	PF13041	113 - 161	2.2e-16	PPR repeat family
PPR	PF01535	116 - 146	1.3e-07	PPR repeat
PPR_3	PF13812	137 - 192	6.9e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	144 - 177	2e-09	PPR repeat
PPR_2	PF13041	148 - 197	4.9e-17	PPR repeat family
PPR	PF01535	151 - 181	2.7e-08	PPR repeat
PPR_long	PF17177	166 - 288	1.4e-12	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	171 - 218	3.5e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	180 - 212	2.5e-06	PPR repeat
PPR_2	PF13041	332 - 376	2.7e-07	PPR repeat family
TPR_24	PF23276	343 - 459	8.1e-08	Fungal tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37230
fragaria_vesca	FvH4_5g34800
malus_domestica	MD08G1198800.v1.1 MD15G1385700.v1.1
prunus_persica	Prupe.1G532500_v2.0.a1
pyrus_communis	pycom08g17270 pycom15g34600
rosa_chinensis	RchiOBHm_Chr6g0274921
rosa_laevigata	RLG00000013509
rosa_multiflora	Rmu_co8266267.1_g000001 Rmu_sc0002311.1_g000019
rosa_roxburghii	Rroxscaffold_1G00028490 Rroxscaffold_7G00193820
rosa_rugosa	Rorug05G0270600 Rorug06G0086200
rosa_samantha	Rh5AG343600 Rh5BG354200 Rh5CG380200 Rh5DG369700 Rh6AG201000 Rh6BG204600 Rh6CG206000 Rh6DG196200
rosa_wichuraiana	Rw5G032350 Rw5G032460 Rw6G017490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	249, 452
AccII	CGCG	1 cut(s)	1272
AciI	CCGC	5 cut(s)	169, 178, 593, 716, 1270
AclWI	GGATC	2 cut(s)	1012, 1193
AcsI	RAATTY	2 cut(s)	568, 1042
AcuI	CTGAAG	1 cut(s)	975
AfaI	GTAC	3 cut(s)	143, 200, 374
AfiI	CCNNNNNNNGG	1 cut(s)	11
AflIII	ACRYGT	1 cut(s)	210
AgsI	TTSAA	9 cut(s)	100, 424, 521, 625, 666, 1009, 1187, 1244, 1259
AjnI	CCWGG	1 cut(s)	577
AjuI	GAANNNNNNNTTGG	2 cut(s)	1301, 1333
AluBI	AGCT	8 cut(s)	94, 388, 611, 844, 953, 1131, 1154, 1169
AluI	AGCT	8 cut(s)	94, 388, 611, 844, 953, 1131, 1154, 1169
Alw21I	GWGCWC	1 cut(s)	732
Alw26I	GTCTC	2 cut(s)	137, 284
AlwI	GGATC	2 cut(s)	1012, 1193
AlwNI	CAGNNNCTG	1 cut(s)	767
AoxI	GGCC	3 cut(s)	66, 399, 869
ApeKI	GCWGC	2 cut(s)	719, 748
ApoI	RAATTY	2 cut(s)	568, 1042
AseI	ATTAAT	2 cut(s)	333, 366
AspLEI	GCGC	1 cut(s)	1411
AspS9I	GGNCC	2 cut(s)	31, 870
AsuHPI	GGTGA	2 cut(s)	340, 1157
AsuII	TTCGAA	1 cut(s)	566
AvaII	GGWCC	1 cut(s)	31
BanII	GRGCYC	1 cut(s)	234
Bbv12I	GWGCWC	1 cut(s)	732
BbvCI	CCTCAGC	1 cut(s)	219
BbvI	GCAGC	2 cut(s)	706, 735
BccI	CCATC	5 cut(s)	181, 385, 497, 625, 1241
BceAI	ACGGC	1 cut(s)	1278
BcgI	CGANNNNNNTGC	2 cut(s)	556, 590
BciT130I	CCWGG	1 cut(s)	579
BciVI	GTATCC	1 cut(s)	791
BcoDI	GTCTC	2 cut(s)	137, 284
BfaI	CTAG	3 cut(s)	237, 897, 1095
BfuI	GTATCC	1 cut(s)	791
BglI	GCCNNNNNGGC	1 cut(s)	238
BisI	GCNGC	4 cut(s)	717, 720, 749, 1270
BlsI	GCNGC	4 cut(s)	718, 721, 750, 1271
Bme1390I	CCNGG	1 cut(s)	579
Bme18I	GGWCC	1 cut(s)	31
BmgT120I	GGNCC	2 cut(s)	31, 870
BmiI	GGNNCC	2 cut(s)	872, 1092
BmrFI	CCNGG	1 cut(s)	579
BmsI	GCATC	3 cut(s)	580, 703, 1147
Bpu10I	CCTNAGC	2 cut(s)	219, 1032
Bpu14I	TTCGAA	1 cut(s)	566
BpuEI	CTTGAG	3 cut(s)	119, 301, 716
BsaJI	CCNNGG	5 cut(s)	578, 654, 934, 1021, 1326
Bsc4I	CCNNNNNNNGG	1 cut(s)	11
Bse118I	RCCGGY	1 cut(s)	68
Bse3DI	GCAATG	2 cut(s)	26, 744
BseBI	CCWGG	1 cut(s)	579
BseDI	CCNNGG	5 cut(s)	578, 654, 934, 1021, 1326
BseGI	GGATG	5 cut(s)	31, 400, 505, 610, 718
BseLI	CCNNNNNNNGG	1 cut(s)	11
BseMI	GCAATG	2 cut(s)	26, 744
BseMII	CTCAG	3 cut(s)	210, 626, 1404
BseXI	GCAGC	2 cut(s)	706, 735
BseYI	CCCAGC	1 cut(s)	806
BsgI	GTGCAG	1 cut(s)	734
Bsh1236I	CGCG	1 cut(s)	1272
BshFI	GGCC	3 cut(s)	68, 401, 871
BsiHKAI	GWGCWC	1 cut(s)	732
BsiSI	CCGG	1 cut(s)	69
BsiWI	CGTACG	1 cut(s)	141
BslI	CCNNNNNNNGG	1 cut(s)	11
BsmAI	GTCTC	2 cut(s)	137, 284
BsnI	GGCC	3 cut(s)	68, 401, 871
Bsp119I	TTCGAA	1 cut(s)	566
Bsp1286I	GDGCHC	2 cut(s)	234, 732
Bsp143I	GATC	4 cut(s)	817, 1017, 1198, 1384
Bsp19I	CCATGG	1 cut(s)	934
BspACI	CCGC	5 cut(s)	169, 178, 593, 716, 1270
BspANI	GGCC	3 cut(s)	68, 401, 871
BspCNI	CTCAG	3 cut(s)	211, 625, 1405
BspFNI	CGCG	1 cut(s)	1272
BspLI	GGNNCC	2 cut(s)	872, 1092
BspPI	GGATC	2 cut(s)	1012, 1193
BspT104I	TTCGAA	1 cut(s)	566
BsrDI	GCAATG	2 cut(s)	26, 744
BsrFI	RCCGGY	1 cut(s)	68
BssAI	RCCGGY	1 cut(s)	68
BssECI	CCNNGG	5 cut(s)	578, 654, 934, 1021, 1326
BssMI	GATC	4 cut(s)	817, 1017, 1198, 1384
BssT1I	CCWWGG	3 cut(s)	654, 934, 1326
Bst2UI	CCWGG	1 cut(s)	579
Bst4CI	ACNGT	3 cut(s)	303, 779, 1178
Bst6I	CTCTTC	4 cut(s)	41, 188, 1078, 1274
BstBI	TTCGAA	1 cut(s)	566
BstC8I	GCNNGC	4 cut(s)	70, 1029, 1267, 1440
BstDEI	CTNAG	5 cut(s)	219, 612, 1032, 1136, 1413
BstDSI	CCRYGG	2 cut(s)	934, 1021
BstF5I	GGATG	5 cut(s)	31, 400, 505, 610, 718
BstFNI	CGCG	1 cut(s)	1272
BstHHI	GCGC	1 cut(s)	1411
BstKTI	GATC	4 cut(s)	820, 1020, 1201, 1387
BstMAI	GTCTC	2 cut(s)	137, 284
BstMBI	GATC	4 cut(s)	817, 1017, 1198, 1384
BstMWI	GCNNNNNNNGC	4 cut(s)	175, 238, 841, 1166
BstNI	CCWGG	1 cut(s)	579
BstNSI	RCATGY	2 cut(s)	214, 1031
BstSCI	CCNGG	1 cut(s)	577
BstUI	CGCG	1 cut(s)	1272
BstV1I	GCAGC	2 cut(s)	706, 735
BstXI	CCANNNNNNTGG	1 cut(s)	1333
BsuI	GTATCC	1 cut(s)	791
BsuRI	GGCC	3 cut(s)	68, 401, 871
BtgI	CCRYGG	2 cut(s)	934, 1021
BtgZI	GCGATG	1 cut(s)	603
BtsCI	GGATG	5 cut(s)	31, 400, 505, 610, 718
BtsIMutI	CAGTG	1 cut(s)	698
Cac8I	GCNNGC	4 cut(s)	70, 1029, 1267, 1440
CaiI	CAGNNNCTG	1 cut(s)	767
CfoI	GCGC	1 cut(s)	1411
Cfr10I	RCCGGY	1 cut(s)	68
Cfr13I	GGNCC	2 cut(s)	31, 870
Csp6I	GTAC	3 cut(s)	142, 199, 373
CviAII	CATG	8 cut(s)	211, 316, 466, 935, 1028, 1196, 1312, 1358
CviQI	GTAC	3 cut(s)	142, 199, 373
DdeI	CTNAG	5 cut(s)	219, 612, 1032, 1136, 1413
DpnI	GATC	4 cut(s)	819, 1019, 1200, 1386
DpnII	GATC	4 cut(s)	817, 1017, 1198, 1384
DraI	TTTAAA	1 cut(s)	1068
Eam1104I	CTCTTC	4 cut(s)	41, 188, 1078, 1274
EarI	CTCTTC	4 cut(s)	41, 188, 1078, 1274
Eco130I	CCWWGG	3 cut(s)	654, 934, 1326
Eco24I	GRGCYC	1 cut(s)	234
Eco47I	GGWCC	1 cut(s)	31
Eco57I	CTGAAG	1 cut(s)	975
EcoO109I	RGGNCCY	2 cut(s)	31, 870
EcoRII	CCWGG	1 cut(s)	577
EcoT14I	CCWWGG	3 cut(s)	654, 934, 1326
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	3 cut(s)	654, 934, 1326
FaeI	CATG	8 cut(s)	214, 319, 469, 938, 1031, 1199, 1315, 1361
FalI	AAGNNNNNCTT	2 cut(s)	185, 217
FatI	CATG	8 cut(s)	210, 315, 465, 934, 1027, 1195, 1311, 1357
Fnu4HI	GCNGC	4 cut(s)	717, 720, 749, 1270
FokI	GGATG	5 cut(s)	38, 407, 512, 617, 725
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	4 cut(s)	717, 720, 749, 1270
FspBI	CTAG	3 cut(s)	237, 897, 1095
GlaI	GCGC	1 cut(s)	1410
GluI	GCNGC	4 cut(s)	717, 720, 749, 1270
GsaI	CCCAGC	1 cut(s)	810
HaeIII	GGCC	3 cut(s)	68, 401, 871
HapII	CCGG	1 cut(s)	69
HhaI	GCGC	1 cut(s)	1411
Hin1II	CATG	8 cut(s)	214, 319, 469, 938, 1031, 1199, 1315, 1361
Hin6I	GCGC	1 cut(s)	1409
HinP1I	GCGC	1 cut(s)	1409
HindIII	AAGCTT	1 cut(s)	92
HinfI	GANTC	7 cut(s)	83, 507, 995, 1049, 1106, 1205, 1234
HpaII	CCGG	1 cut(s)	69
HphI	GGTGA	2 cut(s)	340, 1157
Hpy166II	GTNNAC	2 cut(s)	829, 1148
Hpy188I	TCNGA	5 cut(s)	105, 615, 759, 1048, 1352
Hpy188III	TCNNGA	6 cut(s)	80, 338, 521, 673, 992, 1202
Hpy8I	GTNNAC	2 cut(s)	829, 1148
HpyAV	CCTTC	6 cut(s)	4, 103, 418, 1015, 1031, 1288
HpyCH4III	ACNGT	3 cut(s)	303, 779, 1178
HpyCH4IV	ACGT	1 cut(s)	753
HpyCH4V	TGCA	6 cut(s)	19, 296, 751, 801, 930, 1396
HpyF10VI	GCNNNNNNNGC	4 cut(s)	175, 238, 841, 1166
HpyF3I	CTNAG	5 cut(s)	219, 612, 1032, 1136, 1413
HpySE526I	ACGT	1 cut(s)	753
Hsp92II	CATG	8 cut(s)	214, 319, 469, 938, 1031, 1199, 1315, 1361
HspAI	GCGC	1 cut(s)	1409
KroI	GCCGGC	1 cut(s)	68
KroNI	GCCGGC	1 cut(s)	70
Kzo9I	GATC	4 cut(s)	817, 1017, 1198, 1384
LmnI	GCTCC	2 cut(s)	124, 655
Lsp1109I	GCAGC	2 cut(s)	706, 735
LweI	GCATC	3 cut(s)	580, 703, 1147
MaeI	CTAG	3 cut(s)	237, 897, 1095
MaeII	ACGT	1 cut(s)	753
MaeIII	GTNAC	2 cut(s)	346, 556
MalI	GATC	4 cut(s)	819, 1019, 1200, 1386
MboI	GATC	4 cut(s)	817, 1017, 1198, 1384
MboII	GAAGA	7 cut(s)	28, 205, 262, 661, 1095, 1256, 1291
MhlI	GDGCHC	2 cut(s)	234, 732
MluCI	AATT	9 cut(s)	113, 330, 568, 661, 677, 858, 887, 1012, 1042
MlyI	GAGTC	2 cut(s)	92, 1243
MmeI	TCCRAC	2 cut(s)	267, 782
MroNI	GCCGGC	1 cut(s)	68
MspA1I	CMGCKG	1 cut(s)	1154
MspI	CCGG	1 cut(s)	69
MspR9I	CCNGG	1 cut(s)	579
MvaI	CCWGG	1 cut(s)	579
MvnI	CGCG	1 cut(s)	1272
MwoI	GCNNNNNNNGC	4 cut(s)	175, 238, 841, 1166
NaeI	GCCGGC	1 cut(s)	70
NcoI	CCATGG	1 cut(s)	934
NdeII	GATC	4 cut(s)	817, 1017, 1198, 1384
NgoMIV	GCCGGC	1 cut(s)	68
NlaIII	CATG	8 cut(s)	214, 319, 469, 938, 1031, 1199, 1315, 1361
NlaIV	GGNNCC	2 cut(s)	872, 1092
NmeAIII	GCCGAG	1 cut(s)	427
NmuCI	GTSAC	2 cut(s)	346, 556
NspI	RCATGY	2 cut(s)	214, 1031
NspV	TTCGAA	1 cut(s)	566
PaeI	GCATGC	1 cut(s)	1031
PciI	ACATGT	1 cut(s)	210
PdiI	GCCGGC	1 cut(s)	70
PfeI	GAWTC	5 cut(s)	507, 995, 1049, 1106, 1205
Pfl23II	CGTACG	1 cut(s)	141
PkrI	GCNGC	4 cut(s)	718, 721, 750, 1271
PleI	GAGTC	2 cut(s)	91, 1242
PpsI	GAGTC	2 cut(s)	91, 1242
PpuMI	RGGWCCY	1 cut(s)	31
PscI	ACATGT	1 cut(s)	210
PshBI	ATTAAT	2 cut(s)	333, 366
PsiI	TTATAA	2 cut(s)	249, 452
Psp5II	RGGWCCY	1 cut(s)	31
Psp6I	CCWGG	1 cut(s)	577
PspFI	CCCAGC	1 cut(s)	806
PspGI	CCWGG	1 cut(s)	577
PspLI	CGTACG	1 cut(s)	141
PspN4I	GGNNCC	2 cut(s)	872, 1092
PspPI	GGNCC	2 cut(s)	31, 870
PspPPI	RGGWCCY	1 cut(s)	31
PstNI	CAGNNNCTG	1 cut(s)	767
PvuII	CAGCTG	1 cut(s)	1154
RsaI	GTAC	3 cut(s)	143, 200, 374
RsaNI	GTAC	3 cut(s)	142, 199, 373
SatI	GCNGC	4 cut(s)	717, 720, 749, 1270
Sau3AI	GATC	4 cut(s)	817, 1017, 1198, 1384
Sau96I	GGNCC	2 cut(s)	31, 870
SchI	GAGTC	2 cut(s)	92, 1243
ScrFI	CCNGG	1 cut(s)	579
SduI	GDGCHC	2 cut(s)	234, 732
SfaNI	GCATC	3 cut(s)	580, 703, 1147
SfuI	TTCGAA	1 cut(s)	566
SinI	GGWCC	1 cut(s)	31
SmlI	CTYRAG	3 cut(s)	134, 280, 731
SmoI	CTYRAG	3 cut(s)	134, 280, 731
SphI	GCATGC	1 cut(s)	1031
Sse9I	AATT	9 cut(s)	113, 330, 568, 661, 677, 858, 887, 1012, 1042
SsiI	CCGC	5 cut(s)	169, 178, 593, 716, 1270
SspMI	CTAG	3 cut(s)	237, 897, 1095
StyD4I	CCNGG	1 cut(s)	577
StyI	CCWWGG	3 cut(s)	654, 934, 1326
TaaI	ACNGT	3 cut(s)	303, 779, 1178
TaiI	ACGT	1 cut(s)	756
TaqI	TCGA	4 cut(s)	309, 566, 672, 820
TasI	AATT	9 cut(s)	113, 330, 568, 661, 677, 858, 887, 1012, 1042
TauI	GCSGC	2 cut(s)	719, 1272
TfiI	GAWTC	5 cut(s)	507, 995, 1049, 1106, 1205
TscAI	CASTG	1 cut(s)	698
TseFI	GTSAC	2 cut(s)	346, 556
TseI	GCWGC	2 cut(s)	719, 748
Tsp45I	GTSAC	2 cut(s)	346, 556
TspDTI	ATGAA	9 cut(s)	28, 32, 122, 332, 542, 621, 992, 1292, 1346
TspGWI	ACGGA	1 cut(s)	1010
TspRI	CASTG	1 cut(s)	698
VpaK11BI	GGWCC	1 cut(s)	31
VspI	ATTAAT	2 cut(s)	333, 366
XapI	RAATTY	2 cut(s)	568, 1042
XceI	RCATGY	2 cut(s)	214, 1031
XspI	CTAG	3 cut(s)	237, 897, 1095

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.