Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0006745

Physical Location & Seq

Genomic Coordinates Forward (+)

17417372 .. 17419654

Gene Structure 2283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g17270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGCCATCCATGGCGTACATTTCTCTCTCTAAACCCCATCGATGGCGGCCTCGACCCTCCAATCCTCAGGCCTTCGCGCACCTCCGCCTCTTCAGCTCAGGGGAATCCGCCACCGGTGCCTCTGCTGCCGCAACCAAAGGCGTAGCCGCCACCGAAGCCGCAGCACCAACCGAAGCCGCATCCCCAACTCAAACCCATGTCCCAAAACCTAAGCAGCACCGTCCTCGAAACCCAGAGAAGACAGAGGACATAATCTGCAGAATGATGGCGAATCGGGCTTGGACGACCCGGTTACAGAACTCGATCCGGAACTTGGTACCCGAATTCGAGCACAACCTAGTGTGGAACGTCTTGCACGGCACTGGGAACTGGGAGCACGCCCTCCTATTCTTCAGGTGGGTCGAGCGGTCCGGATTGTTCAAGCACGATCGGGAGACCCATTTGAAGATTATTGAGATTCTGGGCCGGAATTTGAAGCTCAATCATGCCCGGTGCATCCTTCTGGACATGCCGAAAAAAGGTGAGCAGCTCGACGAGGACCTCTTCCTTGCGCTCATTGATGGCTACGGCAAGGCCGGATACGGTAAGGCTGGCATTATTCAAGAATCTGTCAAATTGTTTGACAGTATGAAGGAATTGGGTGTGCAGAGAAGTCTCAAGTCCTACGAGGCTCTCTTTAAGGCCATTATGCGCCGTGGCCGGTATACGATGGCCAAGAGGTATTTTAATGCAATGCTGAGTGAGGGAATAGAGCCCAATAGGCATACTTATAATGTTATGATTTGGGGTTTCTTTTTGAGCAAGAGGTTAGAGACCGCCAAGCGGTTTTATGAGGATATGAAAAGTAGAGGAATTTTGCCTGACCTTGTTACGTATAACACTATGATTCATGGGTACAATCGGTTTAAGATGATGGATGAGGCAGAGCAGTTGTTTGTAGAGTTGAAGGGGAGGAATTTAGAACCTAATGTGATATGCTATACTACCATGATTAAAGGGTATGTTGATGTTGGGAAAGTTGATGATGCATTGAGGTTGTTTCAAGAGATGAAATCTTTTGGTATTAAACCGAATGCTGTCACATTTTCGACTCTATTGCCGGGGCTTTGTGAGGCAGAGAAAAAGGATGAAGCTGTGAACATGTTGAAGGAGATGGTTCAGAGGTATGTTGCTCCCAAGGATAATTCAATCTTTGAGAAATTGTTATCATTGATGTGCAAGTCTGGGGATTTGGATGCAGCTGCTGATGTCCTCAAGGCAATGATTCGGCTGACCATTCCCACCGAGCCTGGTCATTATGGTATCCTGATTGAGAACTTTTGCAAGGCCGGGGTTTATGATCGAGCAATCAAGCTGCTGGATAAGCTTATTGAGAAAGAAATTATTATCAGGCCCCAGAGTTCTATCAAGTTGGAGGCTAGTGCTTATAATCCAATGATTGAGTATTTGTGTAACCATGGGCAGACTGAGAAAGCTGAAGTTTTTTTCCGGCAGTTGATGAAAAAGGGTGTTCAGGATTCAGTTGCCTTCAATAATTTGATGTGCGGACATGCGAAAGAAGGAAATTCTGATTCTACTTTTGAGATTTTAAGGATCATGGGTAGGAGAGGGGTCCCTGGAGAAGCAGATTCTTACAGGTTACTCATTAGCAGCTACTTGAGTAAAGGTGAACCAGCTGATGCTAAAACAGCTTTGGACAGTATGCTTGAAGGCGGGCATATTCCAGAGGCATCACTGTTCAGGTCAGTGATGGAGAGTCTATTTGAAGACGGGAGGGTTCAAACTGCAAGCCGAGTAATGAAAAGTATGGTAGAGAAGGGGGTGAAGGAGAACATGGATTTGGTTGCCAAGATTTTGGAAACCCTCTTTATGAGAGGTCATGTTGAGGAAGCACTGGGACGGATCGATCTACTTATGCAAAGCGGGTGTACGCCTCAGTTTGATAGCCTTTTATCTGTTCTTGCTGAGAAAGGGAAAACAATTGCAGCCCTCAAGCTGTTAGATTTTTGCCTCGAGAGAGATTGCAATGTGGATTTCTCAAGCTATGACAAGGTGTTGGATGCTCTCTTAGAAGCAGGGAAGACTCTTAATGCATACTCAATATTGCGTAAAATAATGGAGAAAGGAGGGGTCTCCGACTGGTGTAGCTCCAAGGATTTGATTAAGAGCCTAAATCAAGAGGGTAACACGAAACAAGCAGATATCCTCTCCAGAATGATAAAGGGAAGGGATAAAAGTGGTCAGAGCAAAAAAGGAAATAAAGAAGCTGTTCTTGCGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031976 GO:0031984 GO:0034357 GO:0034641 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0055035 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

761

Amino Acids

85.57

Weight (kDa)

8.92

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	205 - 261	7.3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	221 - 264	3.2e-09	PPR repeat family
PPR_2	PF13041	256 - 299	2e-13	PPR repeat family
PPR_3	PF13812	277 - 333	5.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	283 - 314	7.5e-09	PPR repeat
PPR_2	PF13041	287 - 335	9.4e-15	PPR repeat family
PPR	PF01535	290 - 319	2.7e-07	PPR repeat
PPR_long	PF17177	305 - 394	2.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	311 - 366	2.4e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	318 - 351	5.1e-10	PPR repeat
PPR_2	PF13041	322 - 370	1.4e-18	PPR repeat family
PPR	PF01535	326 - 355	2e-08	PPR repeat
PPR_long	PF17177	340 - 459	2.7e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	354 - 386	8.9e-09	PPR repeat
TPR_24	PF23276	518 - 633	2.4e-06	Fungal tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37230
fragaria_vesca	FvH4_5g34800
malus_domestica	MD08G1198800.v1.1 MD15G1385700.v1.1
prunus_persica	Prupe.1G532500_v2.0.a1
pyrus_communis	pycom08g17270 pycom15g34600
rosa_chinensis	RchiOBHm_Chr6g0274921
rosa_laevigata	RLG00000013509
rosa_multiflora	Rmu_co8266267.1_g000001 Rmu_sc0002311.1_g000019
rosa_roxburghii	Rroxscaffold_1G00028490 Rroxscaffold_7G00193820
rosa_rugosa	Rorug05G0270600 Rorug06G0086200
rosa_samantha	Rh5AG343600 Rh5BG354200 Rh5CG380200 Rh5DG369700 Rh6AG201000 Rh6BG204600 Rh6CG206000 Rh6DG196200
rosa_wichuraiana	Rw5G032350 Rw5G032460 Rw6G017490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	771, 1428
Acc65I	GGTACC	1 cut(s)	316
AccB1I	GGYRCC	2 cut(s)	116, 316
AccBSI	CCGCTC	1 cut(s)	406
AccI	GTMKAC	1 cut(s)	704
AccII	CGCG	1 cut(s)	77
AccIII	TCCGGA	2 cut(s)	306, 410
AclWI	GGATC	3 cut(s)	298, 1601, 1910
AcoI	YGGCCR	2 cut(s)	697, 711
AcsI	RAATTY	5 cut(s)	323, 469, 852, 955, 1564
AcuI	CTGAAG	3 cut(s)	76, 376, 1497
AfaI	GTAC	4 cut(s)	17, 318, 896, 1930
AfiI	CCNNNNNNNGG	4 cut(s)	42, 313, 518, 822
AflIII	ACRYGT	1 cut(s)	1140
AgeI	ACCGGT	1 cut(s)	113
AjnI	CCWGG	2 cut(s)	1288, 1615
AjuI	GAANNNNNNNTTGG	2 cut(s)	1823, 1855
Alw21I	GWGCWC	2 cut(s)	333, 378
Alw26I	GTCTC	4 cut(s)	428, 659, 806, 2137
AlwI	GGATC	3 cut(s)	298, 1601, 1910
AlwNI	CAGNNNCTG	1 cut(s)	1244
Ama87I	CYCGRG	1 cut(s)	2012
Aor13HI	TCCGGA	2 cut(s)	306, 410
AoxI	GGCC	9 cut(s)	47, 69, 463, 573, 681, 697, 711, 1326, 1391
ApeKI	GCWGC	9 cut(s)	125, 161, 214, 526, 1238, 1241, 1354, 1650, 1985
ApoI	RAATTY	5 cut(s)	323, 469, 852, 955, 1564
AsiGI	ACCGGT	1 cut(s)	113
Asp700I	GAANNNNTTC	1 cut(s)	2268
Asp718I	GGTACC	1 cut(s)	316
AspLEI	GCGC	3 cut(s)	79, 553, 693
AspS9I	GGNCC	5 cut(s)	408, 463, 538, 1392, 1612
AsuC2I	CCSGG	4 cut(s)	289, 490, 1101, 1330
AsuHPI	GGTGA	3 cut(s)	533, 1679, 1834
AvaI	CYCGRG	1 cut(s)	2012
AvaII	GGWCC	3 cut(s)	408, 538, 1612
AxyI	CCTNAGG	1 cut(s)	66
BalI	TGGCCA	1 cut(s)	713
BanI	GGYRCC	2 cut(s)	116, 316
BanII	GRGCYC	1 cut(s)	756
BbsI	GAAGAC	3 cut(s)	245, 1773, 2087
Bbv12I	GWGCWC	2 cut(s)	333, 378
BbvI	GCAGC	9 cut(s)	112, 173, 226, 538, 1228, 1250, 1341, 1662, 1997
BccI	CCATC	9 cut(s)	13, 36, 45, 259, 554, 703, 907, 1147, 1744
BceAI	ACGGC	3 cut(s)	373, 583, 678
BcgI	CGANNNNNNTGC	4 cut(s)	206, 240, 1078, 1112
BciT130I	CCWGG	2 cut(s)	1290, 1617
BciVI	GTATCC	2 cut(s)	572, 1313
BcnI	CCSGG	4 cut(s)	289, 490, 1101, 1330
BcoDI	GTCTC	4 cut(s)	428, 659, 806, 2137
BfaI	CTAG	2 cut(s)	338, 1419
BfmI	CTRYAG	1 cut(s)	256
BfuI	GTATCC	2 cut(s)	572, 1313
BglI	GCCNNNNNGGC	1 cut(s)	760
Bme1390I	CCNGG	6 cut(s)	289, 490, 1101, 1290, 1330, 1617
Bme18I	GGWCC	3 cut(s)	408, 538, 1612
BmeT110I	CYCGRG	1 cut(s)	2012
BmgT120I	GGNCC	5 cut(s)	408, 463, 538, 1392, 1612
BmiI	GGNNCC	5 cut(s)	118, 318, 1394, 1613, 1614
BmrFI	CCNGG	6 cut(s)	289, 490, 1101, 1290, 1330, 1617
BmrI	ACTGGG	3 cut(s)	372, 379, 1904
BmsI	GCATC	7 cut(s)	188, 504, 1015, 1225, 1669, 1739, 2050
BmuI	ACTGGG	3 cut(s)	372, 379, 1904
BpiI	GAAGAC	3 cut(s)	245, 1773, 2087
BpmI	CTGGAG	2 cut(s)	1638, 2194
Bpu10I	CCTNAGC	2 cut(s)	97, 210
BpuEI	CTTGAG	5 cut(s)	641, 1238, 1678, 1976, 2023
BpuMI	CCSGG	4 cut(s)	289, 490, 1101, 1330
Bsa29I	ATCGAT	2 cut(s)	40, 1905
BsaAI	YACGTR	1 cut(s)	873
BsaI	GGTCTC	3 cut(s)	428, 806, 2137
BsaJI	CCNNGG	8 cut(s)	9, 694, 1100, 1176, 1329, 1456, 1615, 2151
BsaWI	WCCGGW	3 cut(s)	113, 306, 410
Bsc4I	CCNNNNNNNGG	4 cut(s)	42, 313, 518, 822
Bse118I	RCCGGY	2 cut(s)	113, 699
Bse1I	ACTGG	4 cut(s)	367, 374, 1899, 2144
Bse21I	CCTNAGG	1 cut(s)	66
Bse3DI	GCAATG	3 cut(s)	738, 1266, 2032
BseAI	TCCGGA	2 cut(s)	306, 410
BseBI	CCWGG	2 cut(s)	1290, 1617
BseCI	ATCGAT	2 cut(s)	40, 1905
BseDI	CCNNGG	8 cut(s)	9, 694, 1100, 1176, 1329, 1456, 1615, 2151
BseGI	GGATG	7 cut(s)	5, 179, 495, 922, 1132, 1240, 2065
BseLI	CCNNNNNNNGG	4 cut(s)	42, 313, 518, 822
BseMI	GCAATG	3 cut(s)	738, 1266, 2032
BseMII	CTCAG	6 cut(s)	80, 111, 728, 1458, 1949, 1956
BseNI	ACTGG	4 cut(s)	367, 374, 1899, 2144
BseXI	GCAGC	9 cut(s)	112, 173, 226, 538, 1228, 1250, 1341, 1662, 1997
BsgI	GTGCAG	1 cut(s)	665
Bsh1236I	CGCG	1 cut(s)	77
Bsh1285I	CGRYCG	1 cut(s)	430
BshFI	GGCC	9 cut(s)	49, 71, 465, 575, 683, 699, 713, 1328, 1393
BshNI	GGYRCC	2 cut(s)	116, 316
BshTI	ACCGGT	1 cut(s)	113
BshVI	ATCGAT	2 cut(s)	40, 1905
BsiEI	CGRYCG	1 cut(s)	430
BsiHKAI	GWGCWC	2 cut(s)	333, 378
BsiHKCI	CYCGRG	1 cut(s)	2012
BslFI	GGGAC	3 cut(s)	185, 1598, 1911
BslI	CCNNNNNNNGG	4 cut(s)	42, 313, 518, 822
BsmAI	GTCTC	4 cut(s)	428, 659, 806, 2137
BsmFI	GGGAC	3 cut(s)	185, 1598, 1911
BsmI	GAATGC	1 cut(s)	1078
BsnI	GGCC	9 cut(s)	49, 71, 465, 575, 683, 699, 713, 1328, 1393
Bso31I	GGTCTC	3 cut(s)	428, 806, 2137
BsoBI	CYCGRG	1 cut(s)	2012
Bsp1286I	GDGCHC	3 cut(s)	333, 378, 756
Bsp13I	TCCGGA	2 cut(s)	306, 410
Bsp143I	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
Bsp19I	CCATGG	2 cut(s)	9, 1456
BspANI	GGCC	9 cut(s)	49, 71, 465, 575, 683, 699, 713, 1328, 1393
BspCNI	CTCAG	6 cut(s)	79, 110, 729, 1459, 1948, 1957
BspDI	ATCGAT	2 cut(s)	40, 1905
BspEI	TCCGGA	2 cut(s)	306, 410
BspFNI	CGCG	1 cut(s)	77
BspLI	GGNNCC	5 cut(s)	118, 318, 1394, 1613, 1614
BspMAI	CTGCAG	1 cut(s)	260
BspPI	GGATC	3 cut(s)	298, 1601, 1910
BspT107I	GGYRCC	2 cut(s)	116, 316
BspTNI	GGTCTC	3 cut(s)	428, 806, 2137
BsrBI	CCGCTC	1 cut(s)	406
BsrDI	GCAATG	3 cut(s)	738, 1266, 2032
BsrFI	RCCGGY	2 cut(s)	113, 699
BsrI	ACTGG	4 cut(s)	367, 374, 1899, 2144
BssAI	RCCGGY	2 cut(s)	113, 699
BssECI	CCNNGG	8 cut(s)	9, 694, 1100, 1176, 1329, 1456, 1615, 2151
BssMI	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
BssNAI	GTATAC	1 cut(s)	705
BssT1I	CCWWGG	4 cut(s)	9, 1176, 1456, 2151
Bst1107I	GTATAC	1 cut(s)	705
Bst2UI	CCWGG	2 cut(s)	1290, 1617
Bst4CI	ACNGT	5 cut(s)	221, 584, 626, 1700, 1737
Bst6I	CTCTTC	2 cut(s)	95, 548
BstBAI	YACGTR	1 cut(s)	873
BstC8I	GCNNGC	4 cut(s)	378, 592, 1715, 1789
BstDEI	CTNAG	8 cut(s)	66, 97, 210, 737, 1467, 1935, 1965, 2068
BstDSI	CCRYGG	3 cut(s)	9, 694, 1456
BstF5I	GGATG	7 cut(s)	5, 179, 495, 922, 1132, 1240, 2065
BstFNI	CGCG	1 cut(s)	77
BstHHI	GCGC	3 cut(s)	79, 553, 693
BstKTI	GATC	6 cut(s)	306, 430, 1342, 1596, 1905, 1909
BstMAI	GTCTC	4 cut(s)	428, 659, 806, 2137
BstMBI	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
BstMCI	CGRYCG	1 cut(s)	430
BstMWI	GCNNNNNNNGC	9 cut(s)	93, 116, 125, 155, 275, 760, 1363, 1688, 2273
BstNI	CCWGG	2 cut(s)	1290, 1617
BstNSI	RCATGY	3 cut(s)	511, 1144, 1553
BstSCI	CCNGG	6 cut(s)	287, 488, 1099, 1288, 1328, 1615
BstSFI	CTRYAG	1 cut(s)	256
BstSNI	TACGTA	1 cut(s)	873
BstUI	CGCG	1 cut(s)	77
BstV1I	GCAGC	9 cut(s)	112, 173, 226, 538, 1228, 1250, 1341, 1662, 1997
BstV2I	GAAGAC	3 cut(s)	245, 1773, 2087
BstXI	CCANNNNNNTGG	1 cut(s)	1855
BstZ17I	GTATAC	1 cut(s)	705
Bsu15I	ATCGAT	2 cut(s)	40, 1905
Bsu36I	CCTNAGG	1 cut(s)	66
BsuI	GTATCC	2 cut(s)	572, 1313
BsuRI	GGCC	9 cut(s)	49, 71, 465, 575, 683, 699, 713, 1328, 1393
BsuTUI	ATCGAT	2 cut(s)	40, 1905
BtgI	CCRYGG	3 cut(s)	9, 694, 1456
BtsCI	GGATG	7 cut(s)	5, 179, 495, 922, 1132, 1240, 2065
BtsIMutI	CAGTG	4 cut(s)	360, 1733, 1752, 1892
Cac8I	GCNNGC	4 cut(s)	378, 592, 1715, 1789
CaiI	CAGNNNCTG	1 cut(s)	1244
CfoI	GCGC	3 cut(s)	79, 553, 693
Cfr10I	RCCGGY	2 cut(s)	113, 699
Cfr13I	GGNCC	5 cut(s)	408, 463, 538, 1392, 1612
ClaI	ATCGAT	2 cut(s)	40, 1905
CpoI	CGGWCCG	1 cut(s)	408
Csp6I	GTAC	4 cut(s)	16, 317, 895, 1929
CspAI	ACCGGT	1 cut(s)	113
CspI	CGGWCCG	1 cut(s)	408
CviQI	GTAC	4 cut(s)	16, 317, 895, 1929
DdeI	CTNAG	8 cut(s)	66, 97, 210, 737, 1467, 1935, 1965, 2068
DpnI	GATC	6 cut(s)	305, 429, 1341, 1595, 1904, 1908
DpnII	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
EaeI	YGGCCR	2 cut(s)	697, 711
Eam1104I	CTCTTC	2 cut(s)	95, 548
EarI	CTCTTC	2 cut(s)	95, 548
EciI	GGCGGA	2 cut(s)	74, 97
Eco105I	TACGTA	1 cut(s)	873
Eco130I	CCWWGG	4 cut(s)	9, 1176, 1456, 2151
Eco147I	AGGCCT	1 cut(s)	71
Eco24I	GRGCYC	1 cut(s)	756
Eco31I	GGTCTC	3 cut(s)	428, 806, 2137
Eco32I	GATATC	1 cut(s)	2203
Eco47I	GGWCC	3 cut(s)	408, 538, 1612
Eco57I	CTGAAG	3 cut(s)	76, 376, 1497
Eco81I	CCTNAGG	1 cut(s)	66
Eco88I	CYCGRG	1 cut(s)	2012
EcoO109I	RGGNCCY	3 cut(s)	538, 1392, 1612
EcoRI	GAATTC	1 cut(s)	323
EcoRII	CCWGG	2 cut(s)	1288, 1615
EcoRV	GATATC	1 cut(s)	2203
EcoT14I	CCWWGG	4 cut(s)	9, 1176, 1456, 2151
EcoT22I	ATGCAT	2 cut(s)	1030, 2095
EcoT38I	GRGCYC	1 cut(s)	756
ErhI	CCWWGG	4 cut(s)	9, 1176, 1456, 2151
FalI	AAGNNNNNCTT	1 cut(s)	2256
FaqI	GGGAC	3 cut(s)	185, 1598, 1911
FauI	CCCGC	2 cut(s)	1706, 1916
FblI	GTMKAC	1 cut(s)	704
FokI	GGATG	6 cut(s)	166, 482, 929, 1139, 1247, 2072
FriOI	GRGCYC	1 cut(s)	756
FspBI	CTAG	2 cut(s)	338, 1419
GlaI	GCGC	3 cut(s)	78, 552, 692
GsuI	CTGGAG	2 cut(s)	1638, 2194
HaeIII	GGCC	9 cut(s)	49, 71, 465, 575, 683, 699, 713, 1328, 1393
HhaI	GCGC	3 cut(s)	79, 553, 693
Hin6I	GCGC	3 cut(s)	77, 551, 691
HinP1I	GCGC	3 cut(s)	77, 551, 691
HindIII	AAGCTT	1 cut(s)	1364
HphI	GGTGA	3 cut(s)	533, 1679, 1834
Hpy166II	GTNNAC	4 cut(s)	705, 1138, 1670, 1929
Hpy188I	TCNGA	4 cut(s)	1161, 1570, 2137, 2244
Hpy8I	GTNNAC	4 cut(s)	705, 1138, 1670, 1929
Hpy99I	CGWCG	1 cut(s)	536
HpyCH4III	ACNGT	5 cut(s)	221, 584, 626, 1700, 1737
HpyCH4IV	ACGT	2 cut(s)	348, 872
HpyF10VI	GCNNNNNNNGC	9 cut(s)	93, 116, 125, 155, 275, 760, 1363, 1688, 2273
HpyF3I	CTNAG	8 cut(s)	66, 97, 210, 737, 1467, 1935, 1965, 2068
HpySE526I	ACGT	2 cut(s)	348, 872
HspAI	GCGC	3 cut(s)	77, 551, 691
KflI	GGGWCCC	1 cut(s)	1612
Kpn2I	TCCGGA	2 cut(s)	306, 410
KpnI	GGTACC	1 cut(s)	320
Kzo9I	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
LmnI	GCTCC	3 cut(s)	373, 1177, 2153
Lsp1109I	GCAGC	9 cut(s)	112, 173, 226, 538, 1228, 1250, 1341, 1662, 1997
LweI	GCATC	7 cut(s)	188, 504, 1015, 1225, 1669, 1739, 2050
MaeI	CTAG	2 cut(s)	338, 1419
MaeII	ACGT	2 cut(s)	348, 872
MaeIII	GTNAC	6 cut(s)	291, 868, 1078, 1451, 1638, 2183
MalI	GATC	6 cut(s)	305, 429, 1341, 1595, 1904, 1908
MbiI	CCGCTC	1 cut(s)	406
MboI	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
MboII	GAAGA	7 cut(s)	82, 250, 382, 457, 535, 1778, 2092
MfeI	CAATTG	1 cut(s)	1980
MhlI	GDGCHC	3 cut(s)	333, 378, 756
MlsI	TGGCCA	1 cut(s)	713
MluNI	TGGCCA	1 cut(s)	713
MlyI	GAGTC	3 cut(s)	1084, 1765, 2077
MmeI	TCCRAC	3 cut(s)	1392, 2037, 2160
Mox20I	TGGCCA	1 cut(s)	713
Mph1103I	ATGCAT	2 cut(s)	1030, 2095
MroI	TCCGGA	2 cut(s)	306, 410
MroXI	GAANNNNTTC	1 cut(s)	2268
MscI	TGGCCA	1 cut(s)	713
MseI	TTAA	8 cut(s)	678, 726, 906, 993, 1065, 1589, 2088, 2163
MslI	CAYNNNNRTG	1 cut(s)	1213
Msp20I	TGGCCA	1 cut(s)	713
MspA1I	CMGCKG	2 cut(s)	1241, 1676
MspR9I	CCNGG	6 cut(s)	289, 490, 1101, 1290, 1330, 1617
MunI	CAATTG	1 cut(s)	1980
Mva1269I	GAATGC	1 cut(s)	1078
MvaI	CCWGG	2 cut(s)	1290, 1617
MvnI	CGCG	1 cut(s)	77
MwoI	GCNNNNNNNGC	9 cut(s)	93, 116, 125, 155, 275, 760, 1363, 1688, 2273
NciI	CCSGG	4 cut(s)	289, 490, 1101, 1330
NcoI	CCATGG	2 cut(s)	9, 1456
NdeII	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
NlaIV	GGNNCC	5 cut(s)	118, 318, 1394, 1613, 1614
NmeAIII	GCCGAG	1 cut(s)	1817
NmuCI	GTSAC	1 cut(s)	1078
NsiI	ATGCAT	2 cut(s)	1030, 2095
NspI	RCATGY	3 cut(s)	511, 1144, 1553
PaeR7I	CTCGAG	1 cut(s)	2012
PceI	AGGCCT	1 cut(s)	71
PciI	ACATGT	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	1078
PdmI	GAANNNNTTC	1 cut(s)	2268
PfeI	GAWTC	9 cut(s)	104, 271, 457, 605, 886, 1264, 1517, 1571, 1628
PinAI	ACCGGT	1 cut(s)	113
Ple19I	CGATCG	1 cut(s)	430
PleI	GAGTC	3 cut(s)	1084, 1764, 2077
PpsI	GAGTC	3 cut(s)	1084, 1764, 2077
Ppu21I	YACGTR	1 cut(s)	873
PpuMI	RGGWCCY	2 cut(s)	538, 1612
PscI	ACATGT	1 cut(s)	1140
PsiI	TTATAA	2 cut(s)	771, 1428
Psp5II	RGGWCCY	2 cut(s)	538, 1612
Psp6I	CCWGG	2 cut(s)	1288, 1615
PspGI	CCWGG	2 cut(s)	1288, 1615
PspN4I	GGNNCC	5 cut(s)	118, 318, 1394, 1613, 1614
PspPI	GGNCC	5 cut(s)	408, 463, 538, 1392, 1612
PspPPI	RGGWCCY	2 cut(s)	538, 1612
PstI	CTGCAG	1 cut(s)	260
PstNI	CAGNNNCTG	1 cut(s)	1244
PvuI	CGATCG	1 cut(s)	430
PvuII	CAGCTG	2 cut(s)	1241, 1676
RsaI	GTAC	4 cut(s)	17, 318, 896, 1930
RsaNI	GTAC	4 cut(s)	16, 317, 895, 1929
RseI	CAYNNNNRTG	1 cut(s)	1213
Rsr2I	CGGWCCG	1 cut(s)	408
RsrII	CGGWCCG	1 cut(s)	408
SaqAI	TTAA	8 cut(s)	678, 726, 906, 993, 1065, 1589, 2088, 2163
Sau3AI	GATC	6 cut(s)	303, 427, 1339, 1593, 1902, 1906
Sau96I	GGNCC	5 cut(s)	408, 463, 538, 1392, 1612
SchI	GAGTC	3 cut(s)	1084, 1765, 2077
ScrFI	CCNGG	6 cut(s)	289, 490, 1101, 1290, 1330, 1617
SduI	GDGCHC	3 cut(s)	333, 378, 756
SfaNI	GCATC	7 cut(s)	188, 504, 1015, 1225, 1669, 1739, 2050
SfcI	CTRYAG	1 cut(s)	256
Sfr274I	CTCGAG	1 cut(s)	2012
SgrAI	CRCCGGYG	1 cut(s)	113
SinI	GGWCC	3 cut(s)	408, 538, 1612
SlaI	CTCGAG	1 cut(s)	2012
SmiMI	CAYNNNNRTG	1 cut(s)	1213
SmlI	CTYRAG	6 cut(s)	656, 1253, 1657, 1991, 2012, 2038
SmoI	CTYRAG	6 cut(s)	656, 1253, 1657, 1991, 2012, 2038
SnaBI	TACGTA	1 cut(s)	873
SseBI	AGGCCT	1 cut(s)	71
SspI	AATATT	1 cut(s)	2103
SspMI	CTAG	2 cut(s)	338, 1419
StuI	AGGCCT	1 cut(s)	71
StyD4I	CCNGG	6 cut(s)	287, 488, 1099, 1288, 1328, 1615
StyI	CCWWGG	4 cut(s)	9, 1176, 1456, 2151
TaaI	ACNGT	5 cut(s)	221, 584, 626, 1700, 1737
TaiI	ACGT	2 cut(s)	351, 875
TauI	GCSGC	5 cut(s)	49, 131, 149, 161, 179
TfiI	GAWTC	9 cut(s)	104, 271, 457, 605, 886, 1264, 1517, 1571, 1628
Tru1I	TTAA	8 cut(s)	678, 726, 906, 993, 1065, 1589, 2088, 2163
Tru9I	TTAA	8 cut(s)	678, 726, 906, 993, 1065, 1589, 2088, 2163
TscAI	CASTG	4 cut(s)	367, 1740, 1752, 1899
TseFI	GTSAC	1 cut(s)	1078
TseI	GCWGC	9 cut(s)	125, 161, 214, 526, 1238, 1241, 1354, 1650, 1985
Tsp45I	GTSAC	1 cut(s)	1078
TspDTI	ATGAA	7 cut(s)	644, 854, 878, 1064, 1143, 1514, 1814
TspGWI	ACGGA	1 cut(s)	1915
TspRI	CASTG	4 cut(s)	367, 1740, 1752, 1899
VpaK11BI	GGWCC	3 cut(s)	408, 538, 1612
XapI	RAATTY	5 cut(s)	323, 469, 852, 955, 1564
XceI	RCATGY	3 cut(s)	511, 1144, 1553
XhoI	CTCGAG	1 cut(s)	2012
XmiI	GTMKAC	1 cut(s)	704
XmnI	GAANNNNTTC	1 cut(s)	2268
XspI	CTAG	2 cut(s)	338, 1419
Zsp2I	ATGCAT	2 cut(s)	1030, 2095

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.