Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001535

Physical Location & Seq

Genomic Coordinates Reverse (-)

27838635 .. 27843311

Gene Structure 4677 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1215500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGATGATAAAGCAACTTCTTAAACTGCACCCTAGCTGTAACTTCCCCTCCCATGGCTTCTGCGTTTACTCAAAGCACTTCTCTTCACTCCCATCTATCACTTCTACCTCTGATATCGAAACCCAATTGAGGTTTCTCTGTGACAAGTCCGACCCTCAGGTTTCGGAGGCGGTTTCGGGTTTCCTCCGCGCCGTCGATACGAACCGCTTGCCTTCTGGGGCAGCCTGTAATTTGCTCGTGCACACTCTCACAAAGTCGAAAAATTATGAATTGGCGTTTTCGGTTTATAGTAAGATGACCAATGTTGGTCTTCGTCCGAGTTTTATATCATTGAGTTGTTTAGTAGCTTGTTTTGTTAACTCCCATCACCCCGAATTCGCTCCCGGGGTGTTGGGATTGTTGTTAAGGCGTGGATTTCAGTTAAATGCGTTTGTTCTGAATCTTACATTGAAGGGTTTGTGCGCTAACGATGAGGTTGACAAGGCGATTGAGTTGTTTCGTGTAACGGGAAGAAATTATGTAACCCCGGATATTGTTAGTTACAACATTCTTATAAACGGACTTTGCAGGGTGAAAAAATTGAAAGAAGCAACTGAGTTGTTGGTTGATATGGAGTTGTTTGGTTCTGAGCCGAATGTGACGACATACAGCACTTTGATCAACGGATTCTGCAAGGATGGTCGAGTGGATGAGGCGATGGGGTTGTTGGAGGAATTGAGGCAGAAGGGTTTGGAAGCGGATGTTGTAGTGTACAGTACCATTATAAGTGCTTTTTGTGATAAAGGGCGTTTCGATAGAGCCAAAGAAATTTTTGATGAGATGTTAAAGAAGGGTATTCCTCCGAATGTGGTTACATATAGTTGTTTCATAAATAATCTCTCCAAGATAGGGAAATGGAAAGAAGCCACCGCGATGTTAAATGATATGATAGAGTGTGGAGTTCTCCCTGATGTTATTACTTATACAGGCTTACTTGACGGGCTTTTCAAAAGTGGAAGGGCTACAAAAGCCATGGAGCTATTCAGTCTAATACTATCGAGGGGTGAAGAGCCTAACACTGTAACATACAATGTTATGATTGATGGACTGTGCAAGCAAGGCTTGGTGGATGAGGCTTTTAAGATTTTTGAAATGATGAAGGGTAAGGGTAAACAGCCTGATGTAATTACATACAACGTGTTACTGATGGGGCTTTCTTGTCGCGGGAAGGTAGATGAGGCAATGGAACTTTTCAGTATGATATCAAAAGATGAGAACTTTCTTGAGCCAGATGTTATCACATATAACATGCTAATTATTGGACTCTGCAAGGAAGGCAACCTTGATAAAGCTATGGATATTTATAATACTATGGTTGAAAGGGGCATTGCAGGTAACTTAGTTACTTATAATGCTTTGATAGGTGCATGTCTAGAGGAAGGATTTGTGGGCGAGGCCATCAAATTCTGGAGACGTGCACTTGACTTGAGATTGGTTCCGAATGAAATTACTTATGGTGTCTTGATCAGTGGATTTTGTAAGACTCATATGCTTAGGTTTGCAAAAGGACTCTTTAGTCAAATGAGAGCTTCTGGGGTTAGTCCTACTTTGATTGACTACAACATTTTGATGCTATCCTTGTGCAAGGAGAGTAGTGTGGAGCAAGCTAGGATGCTATTTGATGAGATGAGGCGTACAAATTGTGTGCCAAATCTGGTCTCGTTTAATACAATAATTGATGGAACTCTAAAAGCAGGAGATGTTGAATCTGCTAAAGGCTTGCTGCAGGACATGTGTAAAATGGATTTAACTCCTGATGCGTTTACCTTTTCCATAGTAGTAAGCCGGTTCTCGAAACTTGGGCTTTTGGATGAGGCTAAAATGGTTCTCAAGAAGATGATTGATTGTGGTGTTGAACCTGATGCCTTTGTTTTCGATTCTTTACTAAAGGGCTATTGTTTAAAGGGTGAAACAGAAGAAATCGTTAATTTGCTTCATCAGATGGCAGACAAGGGTGTTGTTCTCGACTCAGAAATAACTTCTACCATCTTGGAATGCCTTTGTGAAATCTCAGACGATGTTGATGTGATGAAGATTCTCCCAACTTTTTCCCAAGAAACATCAAAAGGAGCAAGCATTACCTGCAATGAGCTATTGGTGAAACTTAGTAACTCTCATCCAGAGCTTAAATTGTCCCACGAGAGACCTCCAGTGCCCGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

734

Amino Acids

81.28

Weight (kDa)

5.44

Isoelectric Point (pI)

30.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	155 - 270	3.3e-06	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	164 - 273	1.1e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	171 - 223	5.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	174 - 205	1.3e-10	PPR repeat
PPR_2	PF13041	176 - 225	1.1e-17	PPR repeat family
PPR	PF01535	179 - 208	5.9e-06	PPR repeat
PPR_1	PF12854	208 - 239	1.7e-14	PPR repeat
PPR_2	PF13041	211 - 259	1.7e-14	PPR repeat family
PPR	PF01535	215 - 244	6.2e-09	PPR repeat
PPR_3	PF13812	235 - 291	1.7e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	242 - 274	2.1e-11	PPR repeat
PPR_2	PF13041	247 - 295	9.4e-17	PPR repeat family
PPR	PF01535	249 - 279	3.2e-08	PPR repeat
PPR_long	PF17177	265 - 350	8e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	277 - 309	4.6e-07	PPR repeat
PPR_2	PF13041	283 - 330	2.4e-12	PPR repeat family
PPR_3	PF13812	306 - 361	2.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	313 - 342	7.2e-07	PPR repeat
PPR_2	PF13041	317 - 365	2e-14	PPR repeat family
TPR_24	PF23276	332 - 450	3e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	348 - 380	2e-14	PPR repeat
PPR_3	PF13812	350 - 395	1.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	351 - 398	1.3e-18	PPR repeat family
PPR	PF01535	354 - 383	1.1e-10	PPR repeat
PPR_long	PF17177	368 - 478	5.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	382 - 412	4.2e-08	PPR repeat
PPR_1	PF12854	421 - 452	2.5e-11	PPR repeat
PPR_3	PF13812	421 - 470	1.3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	423 - 470	1.7e-15	PPR repeat family
PPR	PF01535	426 - 456	2.2e-08	PPR repeat
PPR_2	PF13041	459 - 507	1.7e-10	PPR repeat family
PPR_2	PF13041	493 - 537	4.2e-07	PPR repeat family
PPR_long	PF17177	514 - 678	1.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	525 - 557	4.4e-06	PPR repeat
PPR_2	PF13041	533 - 574	8.1e-11	PPR repeat family
PPR_1	PF12854	560 - 591	3.2e-06	PPR repeat
PPR_2	PF13041	563 - 611	7.8e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28010
fragaria_vesca	FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_5g33200 FvH4_5g33200 FvH4_5g33200
malus_domestica	MD08G1215500.v1.1 MD15G1399800.v1.1 MD15G1400000.v1.1
prunus_persica	Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1
pyrus_communis	pycom08g18660 pycom15g35860
rosa_chinensis	RchiOBHm_Chr5g0048941 RchiOBHm_Chr7g0231581
rosa_laevigata	RLG00000001411 RLG00000001412 RLG00000034570
rosa_multiflora	Rmu_sc0007946.1_g000003
rosa_roxburghii	Rroxscaffold_1G00032970 Rroxscaffold_3G00229080
rosa_rugosa	Rorug05G0241400 Rorug07G0267200 Rorug07G0267300
rosa_samantha	Rh5AG321400 Rh5CG357100 Rh5DG343500 Rh7AG421600 Rh7AG421700 Rh7AG421900 Rh7BG396300 Rh7CG441900 Rh7CG442200 Rh7DG414300
rosa_wichuraiana	Rw5G030330 Rw7G034790 Rw7G034880 Rw7G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	554, 764, 1344, 1389
AasI	GACNNNNNNGTC	1 cut(s)	1554
Acc36I	ACCTGC	2 cut(s)	1361, 2129
AccII	CGCG	3 cut(s)	189, 911, 1203
AciI	CCGC	6 cut(s)	170, 187, 205, 737, 909, 1203
AcsI	RAATTY	3 cut(s)	374, 807, 1442
AfaI	GTAC	3 cut(s)	752, 757, 1675
AfiI	CCNNNNNNNGG	4 cut(s)	53, 129, 888, 2195
AflIII	ACRYGT	2 cut(s)	1176, 1770
AgsI	TTSAA	7 cut(s)	451, 583, 988, 1130, 1358, 1745, 1895
AjiI	CACGTC	1 cut(s)	1454
AjuI	GAANNNNNNNTTGG	2 cut(s)	117, 149
AluBI	AGCT	8 cut(s)	36, 347, 1018, 1331, 1568, 1646, 2131, 2164
AluI	AGCT	8 cut(s)	36, 347, 1018, 1331, 1568, 1646, 2131, 2164
Alw21I	GWGCWC	2 cut(s)	243, 1459
Alw26I	GTCTC	3 cut(s)	1444, 1702, 2176
Alw44I	GTGCAC	2 cut(s)	239, 1455
Ama87I	CYCGRG	1 cut(s)	383
AoxI	GGCC	1 cut(s)	1434
ApaLI	GTGCAC	2 cut(s)	239, 1455
ApeKI	GCWGC	2 cut(s)	221, 1762
ApoI	RAATTY	3 cut(s)	374, 807, 1442
AspLEI	GCGC	2 cut(s)	191, 464
AsuC2I	CCSGG	4 cut(s)	384, 385, 527, 2196
AsuHPI	GGTGA	5 cut(s)	359, 583, 1055, 1958, 2149
AvaI	CYCGRG	1 cut(s)	383
AxyI	CCTNAGG	1 cut(s)	156
BaeGI	GKGCMC	3 cut(s)	243, 1459, 2196
BauI	CACGAG	2 cut(s)	236, 2177
BbsI	GAAGAC	1 cut(s)	302
Bbv12I	GWGCWC	2 cut(s)	243, 1459
BbvI	GCAGC	2 cut(s)	233, 1749
BceAI	ACGGC	1 cut(s)	176
BcgI	CGANNNNNNTGC	2 cut(s)	190, 224
BclI	TGATCA	2 cut(s)	657, 1503
BcnI	CCSGG	4 cut(s)	384, 385, 527, 2196
BcoDI	GTCTC	3 cut(s)	1444, 1702, 2176
BfaI	CTAG	3 cut(s)	33, 1412, 1647
BfmI	CTRYAG	1 cut(s)	1763
BfuAI	ACCTGC	2 cut(s)	1361, 2129
BisI	GCNGC	2 cut(s)	222, 1763
BlsI	GCNGC	2 cut(s)	223, 1764
Bme1390I	CCNGG	4 cut(s)	384, 385, 527, 2196
BmeT110I	CYCGRG	1 cut(s)	383
BmgBI	CACGTC	1 cut(s)	1454
BmiI	GGNNCC	1 cut(s)	1476
BmrFI	CCNGG	4 cut(s)	384, 385, 527, 2196
BmsI	GCATC	4 cut(s)	1599, 1641, 1786, 1891
BpiI	GAAGAC	1 cut(s)	302
BpmI	CTGGAG	2 cut(s)	1468, 2172
Bpu10I	CCTNAGC	1 cut(s)	1532
BpuEI	CTTGAG	3 cut(s)	1283, 1486, 1853
BpuMI	CCSGG	4 cut(s)	384, 385, 527, 2196
BsaI	GGTCTC	2 cut(s)	1702, 2176
BsaJI	CCNNGG	5 cut(s)	52, 383, 384, 525, 1011
BsaXI	ACNNNNNCTCC	2 cut(s)	32, 62
Bsc4I	CCNNNNNNNGG	4 cut(s)	53, 129, 888, 2195
Bse118I	RCCGGY	1 cut(s)	1824
Bse1I	ACTGG	1 cut(s)	2189
Bse21I	CCTNAGG	1 cut(s)	156
Bse3DI	GCAATG	3 cut(s)	1227, 1365, 2131
BseDI	CCNNGG	5 cut(s)	52, 383, 384, 525, 1011
BseGI	GGATG	7 cut(s)	682, 694, 745, 1114, 1656, 1855, 2155
BseLI	CCNNNNNNNGG	4 cut(s)	53, 129, 888, 2195
BseMI	GCAATG	3 cut(s)	1227, 1365, 2131
BseMII	CTCAG	5 cut(s)	170, 585, 618, 2022, 2064
BseNI	ACTGG	1 cut(s)	2189
BseSI	GKGCMC	3 cut(s)	243, 1459, 2196
BseXI	GCAGC	2 cut(s)	233, 1749
BsgI	GTGCAG	1 cut(s)	11
Bsh1236I	CGCG	3 cut(s)	189, 911, 1203
BshFI	GGCC	1 cut(s)	1436
BsiHKAI	GWGCWC	2 cut(s)	243, 1459
BsiHKCI	CYCGRG	1 cut(s)	383
BsiSI	CCGG	4 cut(s)	384, 527, 1825, 2196
BslFI	GGGAC	1 cut(s)	2158
BslI	CCNNNNNNNGG	4 cut(s)	53, 129, 888, 2195
BsmAI	GTCTC	3 cut(s)	1444, 1702, 2176
BsmBI	CGTCTC	1 cut(s)	1444
BsmFI	GGGAC	1 cut(s)	2158
BsmI	GAATGC	1 cut(s)	2039
BsnI	GGCC	1 cut(s)	1436
Bso31I	GGTCTC	2 cut(s)	1702, 2176
BsoBI	CYCGRG	1 cut(s)	383
Bsp1286I	GDGCHC	3 cut(s)	243, 1459, 2196
Bsp1407I	TGTACA	1 cut(s)	750
Bsp143I	GATC	2 cut(s)	657, 1503
Bsp19I	CCATGG	2 cut(s)	52, 1011
BspACI	CCGC	6 cut(s)	170, 187, 205, 737, 909, 1203
BspANI	GGCC	1 cut(s)	1436
BspCNI	CTCAG	5 cut(s)	169, 586, 619, 2021, 2063
BspFNI	CGCG	3 cut(s)	189, 911, 1203
BspLI	GGNNCC	1 cut(s)	1476
BspMAI	CTGCAG	1 cut(s)	1767
BspMI	ACCTGC	2 cut(s)	1361, 2129
BspQI	GCTCTTC	1 cut(s)	1041
BspTNI	GGTCTC	2 cut(s)	1702, 2176
BsrDI	GCAATG	3 cut(s)	1227, 1365, 2131
BsrFI	RCCGGY	1 cut(s)	1824
BsrGI	TGTACA	1 cut(s)	750
BsrI	ACTGG	1 cut(s)	2189
BssAI	RCCGGY	1 cut(s)	1824
BssECI	CCNNGG	5 cut(s)	52, 383, 384, 525, 1011
BssMI	GATC	2 cut(s)	657, 1503
BssSI	CACGAG	2 cut(s)	236, 2177
BssT1I	CCWWGG	2 cut(s)	52, 1011
Bst2BI	CACGAG	2 cut(s)	236, 2177
Bst4CI	ACNGT	3 cut(s)	755, 1060, 1089
Bst6I	CTCTTC	2 cut(s)	88, 1041
BstAPI	GCANNNNNTGC	1 cut(s)	2121
BstAUI	TGTACA	1 cut(s)	750
BstC8I	GCNNGC	5 cut(s)	209, 1094, 1644, 1760, 2113
BstDEI	CTNAG	8 cut(s)	156, 594, 627, 1378, 1532, 2008, 2050, 2144
BstDSI	CCRYGG	2 cut(s)	52, 1011
BstF5I	GGATG	7 cut(s)	682, 694, 745, 1114, 1656, 1855, 2155
BstFNI	CGCG	3 cut(s)	189, 911, 1203
BstHHI	GCGC	2 cut(s)	191, 464
BstKTI	GATC	2 cut(s)	660, 1506
BstMAI	GTCTC	3 cut(s)	1444, 1702, 2176
BstMBI	GATC	2 cut(s)	657, 1503
BstMWI	GCNNNNNNNGC	4 cut(s)	1007, 1098, 1314, 2121
BstNSI	RCATGY	3 cut(s)	1291, 1410, 1774
BstSCI	CCNGG	4 cut(s)	382, 383, 525, 2194
BstSFI	CTRYAG	1 cut(s)	1763
BstSLI	GKGCMC	3 cut(s)	243, 1459, 2196
BstUI	CGCG	3 cut(s)	189, 911, 1203
BstV1I	GCAGC	2 cut(s)	233, 1749
BstV2I	GAAGAC	1 cut(s)	302
Bsu36I	CCTNAGG	1 cut(s)	156
BsuRI	GGCC	1 cut(s)	1436
BtgI	CCRYGG	2 cut(s)	52, 1011
BtgZI	GCGATG	2 cut(s)	710, 926
BtrI	CACGTC	1 cut(s)	1454
BtsCI	GGATG	7 cut(s)	682, 694, 745, 1114, 1656, 1855, 2155
BtsIMutI	CAGTG	3 cut(s)	1056, 1513, 2196
BveI	ACCTGC	2 cut(s)	1361, 2129
Cac8I	GCNNGC	5 cut(s)	209, 1094, 1644, 1760, 2113
CfoI	GCGC	2 cut(s)	191, 464
Cfr10I	RCCGGY	1 cut(s)	1824
Cfr9I	CCCGGG	1 cut(s)	383
Csp6I	GTAC	3 cut(s)	751, 756, 1674
CviAII	CATG	5 cut(s)	53, 1012, 1288, 1407, 1771
CviQI	GTAC	3 cut(s)	751, 756, 1674
DdeI	CTNAG	8 cut(s)	156, 594, 627, 1378, 1532, 2008, 2050, 2144
DpnI	GATC	2 cut(s)	659, 1505
DpnII	GATC	2 cut(s)	657, 1503
DraI	TTTAAA	1 cut(s)	1941
DrdI	GACNNNNNNGTC	1 cut(s)	1554
DseDI	GACNNNNNNGTC	1 cut(s)	1554
Eam1104I	CTCTTC	2 cut(s)	88, 1041
EarI	CTCTTC	2 cut(s)	88, 1041
Eco130I	CCWWGG	2 cut(s)	52, 1011
Eco31I	GGTCTC	2 cut(s)	1702, 2176
Eco32I	GATATC	2 cut(s)	115, 1242
Eco81I	CCTNAGG	1 cut(s)	156
Eco88I	CYCGRG	1 cut(s)	383
EcoRI	GAATTC	1 cut(s)	374
EcoRV	GATATC	2 cut(s)	115, 1242
EcoT14I	CCWWGG	2 cut(s)	52, 1011
ErhI	CCWWGG	2 cut(s)	52, 1011
Esp3I	CGTCTC	1 cut(s)	1444
FaeI	CATG	5 cut(s)	56, 1015, 1291, 1410, 1774
FalI	AAGNNNNNCTT	4 cut(s)	1085, 1117, 1305, 1337
FaqI	GGGAC	1 cut(s)	2158
FatI	CATG	5 cut(s)	52, 1011, 1287, 1406, 1770
FauI	CCCGC	1 cut(s)	1196
FauNDI	CATATG	1 cut(s)	1527
FbaI	TGATCA	2 cut(s)	657, 1503
Fnu4HI	GCNGC	2 cut(s)	222, 1763
FokI	GGATG	7 cut(s)	689, 701, 752, 1121, 1663, 1862, 2142
Fsp4HI	GCNGC	2 cut(s)	222, 1763
FspBI	CTAG	3 cut(s)	33, 1412, 1647
GlaI	GCGC	2 cut(s)	190, 463
GluI	GCNGC	2 cut(s)	222, 1763
GsuI	CTGGAG	2 cut(s)	1468, 2172
HaeIII	GGCC	1 cut(s)	1436
HapII	CCGG	4 cut(s)	384, 527, 1825, 2196
HhaI	GCGC	2 cut(s)	191, 464
Hin1II	CATG	5 cut(s)	56, 1015, 1291, 1410, 1774
Hin6I	GCGC	2 cut(s)	189, 462
HinP1I	GCGC	2 cut(s)	189, 462
HincII	GTYRAC	2 cut(s)	358, 478
HindII	GTYRAC	2 cut(s)	358, 478
HinfI	GANTC	9 cut(s)	439, 666, 1302, 1522, 1548, 1745, 1916, 2006, 2074
HpaI	GTTAAC	1 cut(s)	358
HpaII	CCGG	4 cut(s)	384, 527, 1825, 2196
HphI	GGTGA	5 cut(s)	359, 583, 1055, 1958, 2149
Hpy166II	GTNNAC	8 cut(s)	67, 241, 358, 478, 751, 1151, 1457, 1803
Hpy188III	TCNNGA	9 cut(s)	1262, 1412, 1447, 1501, 1793, 1831, 1870, 2003, 2159
Hpy8I	GTNNAC	8 cut(s)	67, 241, 358, 478, 751, 1151, 1457, 1803
Hpy99I	CGWCG	1 cut(s)	197
HpyAV	CCTTC	9 cut(s)	222, 445, 718, 823, 990, 1132, 1201, 1307, 1412
HpyCH4III	ACNGT	3 cut(s)	755, 1060, 1089
HpyCH4IV	ACGT	2 cut(s)	1176, 1453
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1007, 1098, 1314, 2121
HpyF3I	CTNAG	8 cut(s)	156, 594, 627, 1378, 1532, 2008, 2050, 2144
HpySE526I	ACGT	2 cut(s)	1176, 1453
Hsp92II	CATG	5 cut(s)	56, 1015, 1291, 1410, 1774
HspAI	GCGC	2 cut(s)	189, 462
Ksp22I	TGATCA	2 cut(s)	657, 1503
KspAI	GTTAAC	1 cut(s)	358
Kzo9I	GATC	2 cut(s)	657, 1503
LguI	GCTCTTC	1 cut(s)	1041
LmnI	GCTCC	4 cut(s)	385, 1015, 1639, 2108
Lsp1109I	GCAGC	2 cut(s)	233, 1749
LweI	GCATC	4 cut(s)	1599, 1641, 1786, 1891
MaeI	CTAG	3 cut(s)	33, 1412, 1647
MaeII	ACGT	2 cut(s)	1176, 1453
MalI	GATC	2 cut(s)	659, 1505
MboI	GATC	2 cut(s)	657, 1503
MboII	GAAGA	7 cut(s)	75, 302, 522, 1058, 1885, 1967, 2083
MfeI	CAATTG	1 cut(s)	125
MhlI	GDGCHC	3 cut(s)	243, 1459, 2196
MlyI	GAGTC	4 cut(s)	1296, 1516, 1542, 2000
MmeI	TCCRAC	2 cut(s)	174, 687
MslI	CAYNNNNRTG	2 cut(s)	911, 1607
MspI	CCGG	4 cut(s)	384, 527, 1825, 2196
MspR9I	CCNGG	4 cut(s)	384, 385, 527, 2196
MunI	CAATTG	1 cut(s)	125
Mva1269I	GAATGC	1 cut(s)	2039
MvnI	CGCG	3 cut(s)	189, 911, 1203
MwoI	GCNNNNNNNGC	4 cut(s)	1007, 1098, 1314, 2121
NciI	CCSGG	4 cut(s)	384, 385, 527, 2196
NcoI	CCATGG	2 cut(s)	52, 1011
NdeI	CATATG	1 cut(s)	1527
NdeII	GATC	2 cut(s)	657, 1503
NlaIII	CATG	5 cut(s)	56, 1015, 1291, 1410, 1774
NlaIV	GGNNCC	1 cut(s)	1476
NmuCI	GTSAC	2 cut(s)	140, 637
NspI	RCATGY	3 cut(s)	1291, 1410, 1774
PciI	ACATGT	1 cut(s)	1770
PciSI	GCTCTTC	1 cut(s)	1041
PctI	GAATGC	1 cut(s)	2039
PfeI	GAWTC	5 cut(s)	439, 666, 1745, 1916, 2074
PkrI	GCNGC	2 cut(s)	223, 1764
PleI	GAGTC	4 cut(s)	1296, 1516, 1542, 2000
PpsI	GAGTC	4 cut(s)	1296, 1516, 1542, 2000
PscI	ACATGT	1 cut(s)	1770
PsiI	TTATAA	4 cut(s)	554, 764, 1344, 1389
PspN4I	GGNNCC	1 cut(s)	1476
PstI	CTGCAG	1 cut(s)	1767
RsaI	GTAC	3 cut(s)	752, 757, 1675
RsaNI	GTAC	3 cut(s)	751, 756, 1674
RseI	CAYNNNNRTG	2 cut(s)	911, 1607
SapI	GCTCTTC	1 cut(s)	1041
SatI	GCNGC	2 cut(s)	222, 1763
Sau3AI	GATC	2 cut(s)	657, 1503
SchI	GAGTC	4 cut(s)	1296, 1516, 1542, 2000
ScrFI	CCNGG	4 cut(s)	384, 385, 527, 2196
SduI	GDGCHC	3 cut(s)	243, 1459, 2196
SfaNI	GCATC	4 cut(s)	1599, 1641, 1786, 1891
SfcI	CTRYAG	1 cut(s)	1763
SmaI	CCCGGG	1 cut(s)	385
SmiMI	CAYNNNNRTG	2 cut(s)	911, 1607
SmlI	CTYRAG	3 cut(s)	1262, 1465, 1868
SmoI	CTYRAG	3 cut(s)	1262, 1465, 1868
SsiI	CCGC	6 cut(s)	170, 187, 205, 737, 909, 1203
SspMI	CTAG	3 cut(s)	33, 1412, 1647
StyD4I	CCNGG	4 cut(s)	382, 383, 525, 2194
StyI	CCWWGG	2 cut(s)	52, 1011
TaaI	ACNGT	3 cut(s)	755, 1060, 1089
TaiI	ACGT	2 cut(s)	1179, 1456
TaqI	TCGA	9 cut(s)	117, 195, 257, 682, 792, 1037, 1832, 1914, 2004
TatI	WGTACW	1 cut(s)	750
TfiI	GAWTC	5 cut(s)	439, 666, 1745, 1916, 2074
TscAI	CASTG	3 cut(s)	1063, 1513, 2196
TseFI	GTSAC	2 cut(s)	140, 637
TseI	GCWGC	2 cut(s)	221, 1762
Tsp45I	GTSAC	2 cut(s)	140, 637
TspDTI	ATGAA	6 cut(s)	282, 856, 1151, 1497, 1964, 2084
TspGWI	ACGGA	2 cut(s)	573, 678
TspMI	CCCGGG	1 cut(s)	383
TspRI	CASTG	3 cut(s)	1063, 1513, 2196
VneI	GTGCAC	2 cut(s)	239, 1455
XapI	RAATTY	3 cut(s)	374, 807, 1442
XbaI	TCTAGA	1 cut(s)	1411
XceI	RCATGY	3 cut(s)	1291, 1410, 1774
XmaI	CCCGGG	1 cut(s)	383
XspI	CTAG	3 cut(s)	33, 1412, 1647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.