Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001535

Physical Location & Seq

Genomic Coordinates Reverse (-)

13605741 .. 13608944

Gene Structure 3204 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00229080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGTGGTCATTACAGGCTTCCAAGACACGGCTCTTCTCCTCACTGCCATCTATTACTTCTCTCTCTAATGTAGAAACCCAATTGAGAGCTCTTTTGGATGACTCAGATCCTAAAATTTCAGAGGCAATTTCGATTTTTCATCATGCCATACATTCCAACCGCTTGCCTTCAGGTTCAGCCACAGCCTGCAATTTTCTCGTAGACACTCTCTCAAGGTCGAAAAATTATGAACTAGCATTCTCAGTTTATACTAAGATGACCAAGGTTGGTATTTTTACAAGCTTTGTATCATTGAGTTATTTGGTTTCTTATTTTGTCAATATCTGTAAGCCCGAATTTGCACATGGGGTTTTCAGCTTGGTATTAAAGCGCGGATTTCAGTTAAATGAGTGTGTTATGAATCTTGCATTAAAGGGTTTTTGTTCTAATGATGAAGTTGACAAGGCAATCGAGTTGTTTAGAGTAATGGGAAGACATTTTGTAACGCCTGGCATTCGTAGTTATAACATTCTTATAGATGGGCTTTGCAAAGCAAAAAAGTTGAAAGAAGCAGTGGAATTGTTGGTTGATATGGAGGTGTCAGATTCTGAACCGAATGTGGTGACATACAGCACATTGATTAATGGATTTTGTAAACAGGGTAGAATGGATGAGGCCATGGGTTTCTTGGAGGAAATGAGGCAAAAGGGTTTGGAAAAAGATGTTTTCGTATATAGTGCCCTTATAAGTGGTTTTTGTGCTAAAGGGAGTTTTGATAGAGGAAAACAACTGTTCGATGAGATGTTAAACAAGGGTATTTCTCCTAATGTGGTTACATATAGTTGTTTGATTAATTTTCTTTCAAAGACAGGGAAGTGGAAAGAAGTCACTGTGATGGTAAATGATATGACACAAAGTGGAGTTCCCCCTGATGCTGTTACTTACACAGGTATCCTTGATGGGCTTTTCAAAAATGGAATGGCTACAAAAGCCATGGGGATATTCACTTTAATGCTCATGAAGGGTAAGGAGCCTAATAATGTAACATACAACGTTATGATTGATGGACTATGCAAGGAGGGGTTGATGGATGATGCTTTAAAGATCTTAGAAATGATGAAAGGGAAAGGTGTAAAGCCTGATGTAATTACTTACAACACATTACTAATGGGACTTTTCCGTGATGGGAAGGTTGATGAGGCAATGAAACTTTTCAGTAAGATATCAAACGATGAGAACTTTGTTGAGCCAGATGTTATCACATATAACATGGTCATTTTGGGACTGTGCAAGGAAGGTAATCTTGATGAGGCTATGGAGATTTATCATACAATGATTGAAAGGGGCATTTCTGGTAGCTTATGTACTTATAATACTTTGATAGATCGATGCCTACAGGAAGGATTAGCTGACAAGGCCATAGAATTCTGGAGACATGCAGTGGACTTGGGAGTTATTCCTAATTCAATCACTTACGGTGTCATGATCAATGGATTTTGCAAAAATCAAATGTTCAGGGTTGCAAAAGGACTTTTTACTAAAATGAGAGCTTCTGGGATTAGTCCTACTGTAAATGACTACAACGCTTTGATGTCATCGTTGTGCAAGGAGGGTAGTTTGGGGCAAGCTAGGATGTTGTTTCAAGAAATGAGAACTGCAAATTATTTGCCTAGTGTTGTCTCATTTAATACTATAATTGACGGAATTCTAAAAGCTGGAGATGTTAAATCTGCTAAAGAGCTGCTAGAAGACATGTTCAAATTGGGTTTAACTCCCGATATAATCACATTCTCTACATTAGTAAACCGGTTTTCAAAACTTGGGCTCTTGGATGAGGCTAGAATGTGTGTTGAGAAGATGATTGCTCGTGGTATTGAACCTGATGTCTTTGTATTTGATTCTTTATTAAGGGGCTATAGTTCAAAAGGTGAAACAGAAGAAATTGTTAACTTGCTTCATCAGATGGCAGAGAAGGGTGTTATTCTTGACAAAGAAATAACTTCTACCATCTTGGCGTGCCTCTGTCAAATCTCTGACGATGTTGATGTGATGGAGGTTCTCCCAACTTTTTCTAAAGAGACATCAAAAGGAATAAGCATTACCTGCAATGAGCTATTGATGAAAGTCAATAAATCTTACCCGGAACTTAAGTTACGTGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

714

Amino Acids

79.39

Weight (kDa)

5.84

Isoelectric Point (pI)

24.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	129 - 177	2.4e-10	PPR repeat family
PPR_1	PF12854	162 - 192	1.5e-09	PPR repeat
PPR_2	PF13041	163 - 212	6.6e-17	PPR repeat family
PPR	PF01535	167 - 193	1.6e-06	PPR repeat
PPR_3	PF13812	187 - 242	5.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	197 - 227	7e-16	PPR repeat
PPR_2	PF13041	198 - 246	5.8e-17	PPR repeat family
PPR	PF01535	201 - 231	2e-10	PPR repeat
PPR_3	PF13812	223 - 280	2e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	229 - 261	2.6e-08	PPR repeat
PPR_2	PF13041	233 - 281	1.7e-14	PPR repeat family
PPR	PF01535	237 - 266	2.6e-06	PPR repeat
PPR_long	PF17177	248 - 326	5.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	268 - 317	7.3e-14	PPR repeat family
PPR_3	PF13812	292 - 348	2.1e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	311 - 352	1.2e-10	PPR repeat family
TPR_24	PF23276	318 - 437	1.2e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	334 - 367	1e-12	PPR repeat
PPR_2	PF13041	338 - 386	5.7e-18	PPR repeat family
PPR	PF01535	341 - 371	2.7e-10	PPR repeat
PPR_3	PF13812	361 - 423	1.1e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	369 - 400	7.6e-13	PPR repeat
PPR_2	PF13041	373 - 401	2.4e-07	PPR repeat family
PPR	PF01535	376 - 401	1e-05	PPR repeat
PPR_1	PF12854	408 - 438	6.7e-11	PPR repeat
PPR_3	PF13812	408 - 457	1.2e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	410 - 457	3.3e-14	PPR repeat family
PPR	PF01535	413 - 443	5.1e-08	PPR repeat
PPR_long	PF17177	423 - 536	3.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	448 - 494	2.9e-12	PPR repeat family
PPR_1	PF12854	477 - 509	5.6e-07	PPR repeat
PPR	PF01535	483 - 513	1e-05	PPR repeat
PPR_1	PF12854	512 - 544	5.3e-07	PPR repeat
PPR_2	PF13041	520 - 555	7e-06	PPR repeat family
PPR_long	PF17177	535 - 651	3.4e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	539 - 597	2.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	548 - 578	1.9e-06	PPR repeat
PPR_2	PF13041	550 - 599	1.3e-13	PPR repeat family
PPR_1	PF12854	582 - 613	1e-05	PPR repeat
PPR_2	PF13041	585 - 632	6.5e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28010
fragaria_vesca	FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_5g33200 FvH4_5g33200 FvH4_5g33200
malus_domestica	MD08G1215500.v1.1 MD15G1399800.v1.1 MD15G1400000.v1.1
prunus_persica	Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1
pyrus_communis	pycom08g18660 pycom15g35860
rosa_chinensis	RchiOBHm_Chr5g0048941 RchiOBHm_Chr7g0231581
rosa_laevigata	RLG00000001411 RLG00000001412 RLG00000034570
rosa_multiflora	Rmu_sc0007946.1_g000003
rosa_roxburghii	Rroxscaffold_1G00032970 Rroxscaffold_3G00229080
rosa_rugosa	Rorug05G0241400 Rorug07G0267200 Rorug07G0267300
rosa_samantha	Rh5AG321400 Rh5CG357100 Rh5DG343500 Rh7AG421600 Rh7AG421700 Rh7AG421900 Rh7BG396300 Rh7CG441900 Rh7CG442200 Rh7DG414300
rosa_wichuraiana	Rw5G030330 Rw7G034790 Rw7G034880 Rw7G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	504, 725, 1350
Acc36I	ACCTGC	1 cut(s)	2090
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	1 cut(s)	372
AciI	CCGC	2 cut(s)	160, 372
AclI	AACGTT	1 cut(s)	1032
AclWI	GGATC	1 cut(s)	101
AcsI	RAATTY	4 cut(s)	114, 335, 1403, 1683
AcuI	CTGAAG	1 cut(s)	153
AdeI	CACNNNGTG	1 cut(s)	896
AfaI	GTAC	1 cut(s)	1345
AfiI	CCNNNNNNNGG	1 cut(s)	27
AflII	CTTAAG	1 cut(s)	2126
AflIII	ACRYGT	1 cut(s)	1731
AgeI	ACCGGT	1 cut(s)	1785
AjnI	CCWGG	1 cut(s)	487
Alw21I	GWGCWC	1 cut(s)	91
Alw26I	GTCTC	3 cut(s)	1405, 1663, 2051
AlwI	GGATC	1 cut(s)	101
AlwNI	CAGNNNCTG	1 cut(s)	587
AoxI	GGCC	2 cut(s)	654, 1395
ApeKI	GCWGC	2 cut(s)	1720, 2138
ApoI	RAATTY	4 cut(s)	114, 335, 1403, 1683
AseI	ATTAAT	2 cut(s)	621, 831
AsiGI	ACCGGT	1 cut(s)	1785
Asp700I	GAANNNNTTC	1 cut(s)	1190
AspLEI	GCGC	1 cut(s)	372
AsuC2I	CCSGG	1 cut(s)	2120
AsuHPI	GGTGA	2 cut(s)	613, 1919
BaeGI	GKGCMC	1 cut(s)	721
BaeI	ACNNNNGTAYC	2 cut(s)	913, 946
BanII	GRGCYC	2 cut(s)	91, 1806
BauI	CACGAG	1 cut(s)	1845
BbsI	GAAGAC	2 cut(s)	478, 1734
Bbv12I	GWGCWC	1 cut(s)	91
BbvI	GCAGC	2 cut(s)	1707, 2125
BceAI	ACGGC	1 cut(s)	44
BcgI	CGANNNNNNTGC	2 cut(s)	178, 212
BciT130I	CCWGG	1 cut(s)	489
BciVI	GTATCC	1 cut(s)	941
BclI	TGATCA	1 cut(s)	1464
BcnI	CCSGG	1 cut(s)	2120
BcoDI	GTCTC	3 cut(s)	1405, 1663, 2051
BfaI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
BfmI	CTRYAG	2 cut(s)	1373, 1894
BfrI	CTTAAG	1 cut(s)	2126
BfuAI	ACCTGC	1 cut(s)	2090
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	1083
BisI	GCNGC	2 cut(s)	1721, 2139
BlsI	GCNGC	2 cut(s)	1722, 2140
Bme1390I	CCNGG	2 cut(s)	489, 2120
BmiI	GGNNCC	1 cut(s)	1011
BmrFI	CCNGG	2 cut(s)	489, 2120
BmsI	GCATC	3 cut(s)	901, 1063, 1358
BpiI	GAAGAC	2 cut(s)	478, 1734
BpmI	CTGGAG	2 cut(s)	1429, 1716
BpuEI	CTTGAG	1 cut(s)	196
BpuMI	CCSGG	1 cut(s)	2120
Bsa29I	ATCGAT	1 cut(s)	1366
BsaAI	YACGTR	1 cut(s)	2135
BsaJI	CCNNGG	3 cut(s)	261, 657, 972
BsaWI	WCCGGW	1 cut(s)	1785
Bsc4I	CCNNNNNNNGG	1 cut(s)	27
Bse118I	RCCGGY	1 cut(s)	1785
Bse3DI	GCAATG	2 cut(s)	1188, 2092
BseBI	CCWGG	1 cut(s)	489
BseCI	ATCGAT	1 cut(s)	1366
BseDI	CCNNGG	3 cut(s)	261, 657, 972
BseGI	GGATG	5 cut(s)	103, 655, 1075, 1617, 1816
BseLI	CCNNNNNNNGG	1 cut(s)	27
BseMI	GCAATG	2 cut(s)	1188, 2092
BseMII	CTCAG	2 cut(s)	117, 255
BseRI	GAGGAG	1 cut(s)	28
BseSI	GKGCMC	1 cut(s)	721
BseXI	GCAGC	2 cut(s)	1707, 2125
Bsh1236I	CGCG	1 cut(s)	372
BshFI	GGCC	2 cut(s)	656, 1397
BshTI	ACCGGT	1 cut(s)	1785
BshVI	ATCGAT	1 cut(s)	1366
BsiHKAI	GWGCWC	1 cut(s)	91
BsiSI	CCGG	2 cut(s)	1786, 2120
BslFI	GGGAC	2 cut(s)	1164, 1275
BslI	CCNNNNNNNGG	1 cut(s)	27
BsmAI	GTCTC	3 cut(s)	1405, 1663, 2051
BsmFI	GGGAC	2 cut(s)	1164, 1275
BsmI	GAATGC	2 cut(s)	236, 492
BsnI	GGCC	2 cut(s)	656, 1397
Bsp1286I	GDGCHC	3 cut(s)	91, 721, 1806
Bsp143I	GATC	4 cut(s)	106, 1083, 1363, 1464
Bsp19I	CCATGG	2 cut(s)	657, 972
BspACI	CCGC	2 cut(s)	160, 372
BspANI	GGCC	2 cut(s)	656, 1397
BspCNI	CTCAG	2 cut(s)	116, 254
BspDI	ATCGAT	1 cut(s)	1366
BspFNI	CGCG	1 cut(s)	372
BspHI	TCATGA	2 cut(s)	996, 1461
BspLI	GGNNCC	1 cut(s)	1011
BspMI	ACCTGC	1 cut(s)	2090
BspPI	GGATC	1 cut(s)	101
BspQI	GCTCTTC	1 cut(s)	38
BspTI	CTTAAG	1 cut(s)	2126
BsrDI	GCAATG	2 cut(s)	1188, 2092
BsrFI	RCCGGY	1 cut(s)	1785
BssAI	RCCGGY	1 cut(s)	1785
BssECI	CCNNGG	3 cut(s)	261, 657, 972
BssMI	GATC	4 cut(s)	106, 1083, 1363, 1464
BssSI	CACGAG	1 cut(s)	1845
BssT1I	CCWWGG	3 cut(s)	261, 657, 972
Bst2BI	CACGAG	1 cut(s)	1845
Bst2UI	CCWGG	1 cut(s)	489
Bst4CI	ACNGT	5 cut(s)	771, 871, 1266, 1457, 1549
Bst6I	CTCTTC	1 cut(s)	38
BstAFI	CTTAAG	1 cut(s)	2126
BstAPI	GCANNNNNTGC	1 cut(s)	2082
BstBAI	YACGTR	1 cut(s)	2135
BstC8I	GCNNGC	4 cut(s)	164, 187, 1605, 1996
BstDEI	CTNAG	4 cut(s)	103, 241, 252, 1087
BstDSI	CCRYGG	2 cut(s)	657, 972
BstF5I	GGATG	5 cut(s)	103, 655, 1075, 1617, 1816
BstFNI	CGCG	1 cut(s)	372
BstHHI	GCGC	1 cut(s)	372
BstKTI	GATC	4 cut(s)	109, 1086, 1366, 1467
BstMAI	GTCTC	3 cut(s)	1405, 1663, 2051
BstMBI	GATC	4 cut(s)	106, 1083, 1363, 1464
BstMWI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
BstNI	CCWGG	1 cut(s)	489
BstNSI	RCATGY	2 cut(s)	1418, 1735
BstSCI	CCNGG	2 cut(s)	487, 2118
BstSFI	CTRYAG	2 cut(s)	1373, 1894
BstSLI	GKGCMC	1 cut(s)	721
BstUI	CGCG	1 cut(s)	372
BstV1I	GCAGC	2 cut(s)	1707, 2125
BstV2I	GAAGAC	2 cut(s)	478, 1734
BstX2I	RGATCY	2 cut(s)	106, 1083
BstYI	RGATCY	2 cut(s)	106, 1083
Bsu15I	ATCGAT	1 cut(s)	1366
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	2 cut(s)	656, 1397
BsuTUI	ATCGAT	1 cut(s)	1366
BtgI	CCRYGG	2 cut(s)	657, 972
BtsCI	GGATG	5 cut(s)	103, 655, 1075, 1617, 1816
BtsI	GCAGTG	3 cut(s)	41, 558, 1425
BtsIMutI	CAGTG	4 cut(s)	41, 558, 867, 1425
BveI	ACCTGC	1 cut(s)	2090
Cac8I	GCNNGC	4 cut(s)	164, 187, 1605, 1996
CaiI	CAGNNNCTG	1 cut(s)	587
CciI	TCATGA	2 cut(s)	996, 1461
CfoI	GCGC	1 cut(s)	372
Cfr10I	RCCGGY	1 cut(s)	1785
ClaI	ATCGAT	1 cut(s)	1366
Csp6I	GTAC	1 cut(s)	1344
CspAI	ACCGGT	1 cut(s)	1785
CviAII	CATG	9 cut(s)	143, 344, 658, 973, 997, 1249, 1415, 1462, 1732
CviQI	GTAC	1 cut(s)	1344
DdeI	CTNAG	4 cut(s)	103, 241, 252, 1087
DpnI	GATC	4 cut(s)	108, 1085, 1365, 1466
DpnII	GATC	4 cut(s)	106, 1083, 1363, 1464
DraI	TTTAAA	1 cut(s)	1080
DraIII	CACNNNGTG	1 cut(s)	896
Eam1104I	CTCTTC	1 cut(s)	38
EarI	CTCTTC	1 cut(s)	38
Ecl136II	GAGCTC	1 cut(s)	89
Eco130I	CCWWGG	3 cut(s)	261, 657, 972
Eco24I	GRGCYC	2 cut(s)	91, 1806
Eco32I	GATATC	1 cut(s)	1203
Eco53kI	GAGCTC	1 cut(s)	89
Eco57I	CTGAAG	1 cut(s)	153
EcoICRI	GAGCTC	1 cut(s)	89
EcoRI	GAATTC	2 cut(s)	1403, 1683
EcoRII	CCWGG	1 cut(s)	487
EcoRV	GATATC	1 cut(s)	1203
EcoT14I	CCWWGG	3 cut(s)	261, 657, 972
EcoT38I	GRGCYC	2 cut(s)	91, 1806
ErhI	CCWWGG	3 cut(s)	261, 657, 972
FaeI	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	2 cut(s)	1164, 1275
FatI	CATG	9 cut(s)	142, 343, 657, 972, 996, 1248, 1414, 1461, 1731
FbaI	TGATCA	1 cut(s)	1464
FblI	GTMKAC	1 cut(s)	201
Fnu4HI	GCNGC	2 cut(s)	1721, 2139
FokI	GGATG	5 cut(s)	110, 662, 1082, 1624, 1823
FriOI	GRGCYC	2 cut(s)	91, 1806
Fsp4HI	GCNGC	2 cut(s)	1721, 2139
FspBI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
GlaI	GCGC	1 cut(s)	371
GluI	GCNGC	2 cut(s)	1721, 2139
GsuI	CTGGAG	2 cut(s)	1429, 1716
HaeIII	GGCC	2 cut(s)	656, 1397
HapII	CCGG	2 cut(s)	1786, 2120
HhaI	GCGC	1 cut(s)	372
Hin1II	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
Hin6I	GCGC	1 cut(s)	370
HinP1I	GCGC	1 cut(s)	370
HincII	GTYRAC	2 cut(s)	439, 1927
HindII	GTYRAC	2 cut(s)	439, 1927
HindIII	AAGCTT	1 cut(s)	280
HinfI	GANTC	4 cut(s)	101, 400, 584, 1877
HpaI	GTTAAC	1 cut(s)	1927
HpaII	CCGG	2 cut(s)	1786, 2120
HphI	GGTGA	2 cut(s)	613, 1919
Hpy166II	GTNNAC	6 cut(s)	202, 439, 635, 1423, 1783, 1927
Hpy188I	TCNGA	6 cut(s)	106, 121, 583, 589, 1941, 2014
Hpy188III	TCNNGA	7 cut(s)	997, 1283, 1408, 1462, 1622, 1754, 1964
Hpy8I	GTNNAC	6 cut(s)	202, 439, 635, 1423, 1783, 1927
HpyAV	CCTTC	6 cut(s)	177, 994, 1162, 1268, 1373, 1945
HpyCH4III	ACNGT	5 cut(s)	771, 871, 1266, 1457, 1549
HpyCH4IV	ACGT	2 cut(s)	1032, 2134
HpyF10VI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
HpyF3I	CTNAG	4 cut(s)	103, 241, 252, 1087
HpySE526I	ACGT	2 cut(s)	1032, 2134
Hsp92II	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
HspAI	GCGC	1 cut(s)	370
Ksp22I	TGATCA	1 cut(s)	1464
KspAI	GTTAAC	1 cut(s)	1927
Kzo9I	GATC	4 cut(s)	106, 1083, 1363, 1464
LguI	GCTCTTC	1 cut(s)	38
LmnI	GCTCC	1 cut(s)	1009
Lsp1109I	GCAGC	2 cut(s)	1707, 2125
LweI	GCATC	3 cut(s)	901, 1063, 1358
MaeI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
MaeII	ACGT	2 cut(s)	1032, 2134
MaeIII	GTNAC	7 cut(s)	481, 601, 811, 865, 916, 1021, 2130
MalI	GATC	4 cut(s)	108, 1085, 1365, 1466
MboI	GATC	4 cut(s)	106, 1083, 1363, 1464
MboII	GAAGA	5 cut(s)	25, 483, 1739, 1846, 1928
MfeI	CAATTG	1 cut(s)	80
MflI	RGATCY	2 cut(s)	106, 1083
MhlI	GDGCHC	3 cut(s)	91, 721, 1806
MlyI	GAGTC	1 cut(s)	95
MmeI	TCCRAC	1 cut(s)	180
MroXI	GAANNNNTTC	1 cut(s)	1190
MslI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
MspCI	CTTAAG	1 cut(s)	2126
MspI	CCGG	2 cut(s)	1786, 2120
MspR9I	CCNGG	2 cut(s)	489, 2120
MunI	CAATTG	1 cut(s)	80
Mva1269I	GAATGC	2 cut(s)	236, 492
MvaI	CCWGG	1 cut(s)	489
MvnI	CGCG	1 cut(s)	372
MwoI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
NciI	CCSGG	1 cut(s)	2120
NcoI	CCATGG	2 cut(s)	657, 972
NdeII	GATC	4 cut(s)	106, 1083, 1363, 1464
NlaIII	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
NlaIV	GGNNCC	1 cut(s)	1011
NmuCI	GTSAC	2 cut(s)	601, 865
NspI	RCATGY	2 cut(s)	1418, 1735
PagI	TCATGA	2 cut(s)	996, 1461
PciI	ACATGT	1 cut(s)	1731
PciSI	GCTCTTC	1 cut(s)	38
PctI	GAATGC	2 cut(s)	236, 492
PdmI	GAANNNNTTC	1 cut(s)	1190
PfeI	GAWTC	3 cut(s)	400, 584, 1877
PinAI	ACCGGT	1 cut(s)	1785
PkrI	GCNGC	2 cut(s)	1722, 2140
PleI	GAGTC	1 cut(s)	95
PpsI	GAGTC	1 cut(s)	95
Ppu21I	YACGTR	1 cut(s)	2135
PscI	ACATGT	1 cut(s)	1731
PshBI	ATTAAT	2 cut(s)	621, 831
PsiI	TTATAA	3 cut(s)	504, 725, 1350
Psp124BI	GAGCTC	1 cut(s)	91
Psp1406I	AACGTT	1 cut(s)	1032
Psp6I	CCWGG	1 cut(s)	487
PspGI	CCWGG	1 cut(s)	487
PspN4I	GGNNCC	1 cut(s)	1011
PsrI	GAACNNNNNNTAC	1 cut(s)	2115
PstNI	CAGNNNCTG	1 cut(s)	587
PsuI	RGATCY	2 cut(s)	106, 1083
RsaI	GTAC	1 cut(s)	1345
RsaNI	GTAC	1 cut(s)	1344
RseI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
SacI	GAGCTC	1 cut(s)	91
SapI	GCTCTTC	1 cut(s)	38
SatI	GCNGC	2 cut(s)	1721, 2139
Sau3AI	GATC	4 cut(s)	106, 1083, 1363, 1464
SchI	GAGTC	1 cut(s)	95
ScrFI	CCNGG	2 cut(s)	489, 2120
SduI	GDGCHC	3 cut(s)	91, 721, 1806
SfaNI	GCATC	3 cut(s)	901, 1063, 1358
SfcI	CTRYAG	2 cut(s)	1373, 1894
SmiMI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
SmlI	CTYRAG	2 cut(s)	211, 2126
SmoI	CTYRAG	2 cut(s)	211, 2126
SsiI	CCGC	2 cut(s)	160, 372
SspMI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
SstI	GAGCTC	1 cut(s)	91
StyD4I	CCNGG	2 cut(s)	487, 2118
StyI	CCWWGG	3 cut(s)	261, 657, 972
TaaI	ACNGT	5 cut(s)	771, 871, 1266, 1457, 1549
TaiI	ACGT	2 cut(s)	1035, 2137
TaqI	TCGA	5 cut(s)	131, 218, 450, 774, 1366
TatI	WGTACW	1 cut(s)	1343
TfiI	GAWTC	3 cut(s)	400, 584, 1877
TscAI	CASTG	4 cut(s)	48, 558, 874, 1425
TseFI	GTSAC	2 cut(s)	601, 865
TseI	GCWGC	2 cut(s)	1720, 2138
Tsp45I	GTSAC	2 cut(s)	601, 865
TspDTI	ATGAA	9 cut(s)	128, 243, 413, 447, 1013, 1112, 1199, 1925, 2114
TspGWI	ACGGA	2 cut(s)	1148, 1695
TspRI	CASTG	4 cut(s)	48, 558, 874, 1425
Vha464I	CTTAAG	1 cut(s)	2126
VspI	ATTAAT	2 cut(s)	621, 831
XapI	RAATTY	4 cut(s)	114, 335, 1403, 1683
XceI	RCATGY	2 cut(s)	1418, 1735
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XmiI	GTMKAC	1 cut(s)	201
XmnI	GAANNNNTTC	1 cut(s)	1190
XspI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.