Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0001535

Physical Location & Seq

Genomic Coordinates Forward (+)

57955919 .. 57959638

Gene Structure 3720 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG442200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGTGGTCATTACAGGCTTCCAAGACACGGCTCTTCTCCTCACTGCCATCTATTACTTCTCTCTCGAATGTAGAAACCCAATTGAGAGCTCTTTTGGAGGACCCAGATCCTAAAATTTCACAGGCAATTTCGATTTTTCATCATGCCATACATTCCAACCGCTTGCCTTCAGGTTCAGCCGCGGCCTGCAATTTTCTCATAGACACTCTCTCAAGGTCGAAAAATTATGAACTAGCATTCTCAGTTTATACTAAGATGACCAAGGTTGGTATTTTTACAAGCTTTGTATCATTGAGTTGTTTGGTTTCATATTTTGTCAATATCCGTAAGCCCGAATTTGCACATGGGGTTTTCTGCTTGGTATTAAAGCGTGGATTTCAGTTAAATGAGTGTGTTATGAATCTTGCATTAAAGGGTTTTTGTTCGAATGATGAAGTTGACAAGGCAATCGATTTGTTTAGAGTAATGGGAAGACATTTTGTAACGCCTGGCATTCGTAGTTATAACATTCTTATAGATGGACTTTGCAAAGCAAAAAGGTTGAAAGAAGCAGTGGAATTGTTGGTTGATATGGAGGTGTCAGATTCTGAACCGAATGTGGTAACGTACAGCACATTGATTAATGGATTTTGTAAACAGGGTAGAATGGACGAGGCCATGGGTTTCTTGGAGGAAATGAGGCAAAAGGGTTTGGAAATAGATGTTTTCGTATATAGTGCCCTTATAAGTGGTTTTTGTGCTAAAGGGAGTTTTGATAGAGGAAAACAACTGTTCGATGAGATGTTAAACAACGGTATTTCTCCTAATGTGGTTACATATAGTTGTTTGATTAATTTTCTTTCAAAGGCAGGGAAGTGGAAAGAAGTCACTGTGATGATAAATGATATGACACAAAGTGGAGTTCCCCCTGATGCTGTTACTTACACAGGTATCCTTGATGGGCTTTTCAAAAATGGAATGGCTACAAAAGCCATGGGGATATTCACTTTAATGCTCATGAAGGGTAAAGAGCCTAATAATGTAACATACAACGTTATGATTGATGGACTATGCAAGGAGGGGTTGATGGATGATGCTTTAAAGATCTTAGAAATGATGAAAGGGAAAGGTGTAAAGCCTGATGTAATTACTTACAACACATTACTAATGGGACTATTCTGTGATGGGAAGGTTGATGAGGCAATGAAACTTTTCAGTAAGATAACAAACGATGAGAACTTTGTCAAGCCAGATGTTATCACATATAACATGGTCATTTTGGGACTGTGCAAGGAAGGTAATCTTGATGAGGCTATGGAGATTTATCATACAATGATTGAAAGGGGCATTTCTGGTAGCTTATGTACTTATAATACTTTGATAGATAGATGCCTACAGGAAGGATTAGCTGACAAGGCCATAGAATTCTGGAGACATGCAGTGGACTTGGGAGTTATTCCTAACTCAATCACTTATGGTGTCATGATTAATGGATTTTGCAAAAATCAAATGTTCAGGGTTGCAAAAGGACTTTTTACTAAAATGAGAGCTTCTGGGATTAGTCCGACTGTAAATGACTACAATGCTTTGATGTCATCGTTGTGCAAGGAGGGTAGTTTGGGGCAAGCTAGGATATTGTTTCAAGAAATGAGAATGGTAAATTATTTGCCTAGTCTTGTCTCATTTAATACTATAATTGACGGAATTCTAAAAGCTGGAGATGTTAAATCTGCTAAAGAGTTGCTAGAAGACATGTTCAAATTGGGTTTAACTCCCGATATAATTACATTCTCTACATTAGTAAACCGGTTTTCAAAACTTGGGCTCTTGGATGAGGCTAGAATGTGTGTTGAGAAGATGATTGCTCGTGGTTTTGAACCTGATGTCTTTGTATTTGATTCTTTATTAAAGGGCTATAGTTCAAAAGGTGAAACAGAAGAAATTGTTAACTTGCTTCATCAGATGGCAGACAAGGGTGTTATTCTTGACAAAGAAATAACTTCTACCATCTTGGCGTGCCTCTGTCAAATCTCTGACGATGTTGATGTGATGGAGGTTCTCCCAACTTTTTCCAAAGAGACATCAAAAGGAATAAGCATTACCTGCAATGAGCTATTGATGAAAGTCAATCAATCTTACCCGGAACTTAAGTTACGTGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

714

Amino Acids

79.38

Weight (kDa)

5.83

Isoelectric Point (pI)

24.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	61 - 228	2.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	129 - 177	2.3e-10	PPR repeat family
PPR_1	PF12854	162 - 192	7.8e-10	PPR repeat
PPR_2	PF13041	163 - 212	8.7e-17	PPR repeat family
PPR	PF01535	167 - 193	1.7e-06	PPR repeat
PPR_1	PF12854	197 - 227	7e-16	PPR repeat
PPR_2	PF13041	198 - 246	5.6e-18	PPR repeat family
PPR	PF01535	201 - 231	2e-10	PPR repeat
PPR_3	PF13812	223 - 280	6.8e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	229 - 261	1.1e-08	PPR repeat
PPR_2	PF13041	234 - 281	5e-14	PPR repeat family
PPR	PF01535	237 - 266	3.5e-06	PPR repeat
PPR_long	PF17177	250 - 327	5.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	269 - 317	3.6e-13	PPR repeat family
PPR_3	PF13812	293 - 348	3.3e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	306 - 352	7.1e-13	PPR repeat family
TPR_24	PF23276	318 - 437	6.6e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	334 - 367	1e-12	PPR repeat
PPR_2	PF13041	338 - 385	8.3e-18	PPR repeat family
PPR	PF01535	341 - 371	2.7e-10	PPR repeat
PPR_3	PF13812	361 - 423	2.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	369 - 400	4.8e-12	PPR repeat
PPR_2	PF13041	373 - 401	6.1e-06	PPR repeat family
PPR_1	PF12854	408 - 438	5.1e-11	PPR repeat
PPR_3	PF13812	408 - 457	8.4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	410 - 457	3.3e-14	PPR repeat family
PPR	PF01535	413 - 443	5.1e-08	PPR repeat
PPR_long	PF17177	423 - 539	1.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	448 - 494	2.9e-12	PPR repeat family
PPR_1	PF12854	477 - 509	5.6e-07	PPR repeat
PPR	PF01535	483 - 513	1e-05	PPR repeat
PPR_1	PF12854	512 - 544	4.7e-06	PPR repeat
PPR_long	PF17177	537 - 651	7.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	539 - 597	2.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	550 - 599	1e-12	PPR repeat family
PPR_1	PF12854	582 - 613	1e-05	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28010
fragaria_vesca	FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_5g33200 FvH4_5g33200 FvH4_5g33200
malus_domestica	MD08G1215500.v1.1 MD15G1399800.v1.1 MD15G1400000.v1.1
prunus_persica	Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1
pyrus_communis	pycom08g18660 pycom15g35860
rosa_chinensis	RchiOBHm_Chr5g0048941 RchiOBHm_Chr7g0231581
rosa_laevigata	RLG00000001411 RLG00000001412 RLG00000034570
rosa_multiflora	Rmu_sc0007946.1_g000003
rosa_roxburghii	Rroxscaffold_1G00032970 Rroxscaffold_3G00229080
rosa_rugosa	Rorug05G0241400 Rorug07G0267200 Rorug07G0267300
rosa_samantha	Rh5AG321400 Rh5CG357100 Rh5DG343500 Rh7AG421600 Rh7AG421700 Rh7AG421900 Rh7BG396300 Rh7CG441900 Rh7CG442200 Rh7DG414300
rosa_wichuraiana	Rw5G030330 Rw7G034790 Rw7G034880 Rw7G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	504, 725, 1350
Acc36I	ACCTGC	1 cut(s)	2090
AccII	CGCG	1 cut(s)	182
AciI	CCGC	3 cut(s)	160, 180, 182
AclI	AACGTT	1 cut(s)	1032
AclWI	GGATC	1 cut(s)	101
AcsI	RAATTY	4 cut(s)	114, 335, 1403, 1683
AcuI	CTGAAG	1 cut(s)	153
AdeI	CACNNNGTG	1 cut(s)	896
AfaI	GTAC	2 cut(s)	608, 1345
AfiI	CCNNNNNNNGG	1 cut(s)	27
AflII	CTTAAG	1 cut(s)	2126
AflIII	ACRYGT	1 cut(s)	1731
AgeI	ACCGGT	1 cut(s)	1785
AgsI	TTSAA	9 cut(s)	544, 843, 949, 1319, 1622, 1738, 1794, 1856, 1902
AjnI	CCWGG	1 cut(s)	487
AluBI	AGCT	8 cut(s)	89, 282, 1338, 1388, 1529, 1607, 1694, 2092
AluI	AGCT	8 cut(s)	89, 282, 1338, 1388, 1529, 1607, 1694, 2092
Alw21I	GWGCWC	1 cut(s)	91
Alw26I	GTCTC	3 cut(s)	1405, 1663, 2051
AlwI	GGATC	1 cut(s)	101
AlwNI	CAGNNNCTG	1 cut(s)	587
AoxI	GGCC	3 cut(s)	183, 654, 1395
ApeKI	GCWGC	1 cut(s)	2138
ApoI	RAATTY	4 cut(s)	114, 335, 1403, 1683
AseI	ATTAAT	3 cut(s)	621, 831, 1467
AsiGI	ACCGGT	1 cut(s)	1785
Asp700I	GAANNNNTTC	1 cut(s)	1190
AspS9I	GGNCC	1 cut(s)	100
AsuC2I	CCSGG	1 cut(s)	2120
AsuHPI	GGTGA	1 cut(s)	1919
AsuII	TTCGAA	1 cut(s)	425
AvaII	GGWCC	1 cut(s)	100
BaeGI	GKGCMC	1 cut(s)	721
BaeI	ACNNNNGTAYC	2 cut(s)	913, 946
BanII	GRGCYC	2 cut(s)	91, 1806
BauI	CACGAG	1 cut(s)	1845
BbsI	GAAGAC	2 cut(s)	478, 1734
Bbv12I	GWGCWC	1 cut(s)	91
BbvI	GCAGC	1 cut(s)	2125
BccI	CCATC	9 cut(s)	55, 512, 932, 1037, 1060, 1157, 1936, 1994, 2023
BceAI	ACGGC	1 cut(s)	44
BciT130I	CCWGG	1 cut(s)	489
BciVI	GTATCC	1 cut(s)	941
BcnI	CCSGG	1 cut(s)	2120
BcoDI	GTCTC	3 cut(s)	1405, 1663, 2051
BfaI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
BfmI	CTRYAG	2 cut(s)	1373, 1894
BfrI	CTTAAG	1 cut(s)	2126
BfuAI	ACCTGC	1 cut(s)	2090
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	1083
BisI	GCNGC	3 cut(s)	180, 183, 2139
BlsI	GCNGC	3 cut(s)	181, 184, 2140
Bme1390I	CCNGG	2 cut(s)	489, 2120
Bme18I	GGWCC	1 cut(s)	100
BmgT120I	GGNCC	1 cut(s)	100
BmiI	GGNNCC	1 cut(s)	102
BmrFI	CCNGG	2 cut(s)	489, 2120
BmsI	GCATC	3 cut(s)	901, 1063, 1358
BpiI	GAAGAC	2 cut(s)	478, 1734
BpmI	CTGGAG	2 cut(s)	1429, 1716
Bpu14I	TTCGAA	1 cut(s)	425
BpuEI	CTTGAG	1 cut(s)	196
BpuMI	CCSGG	1 cut(s)	2120
Bsa29I	ATCGAT	1 cut(s)	450
BsaAI	YACGTR	1 cut(s)	2135
BsaJI	CCNNGG	4 cut(s)	180, 261, 657, 972
BsaWI	WCCGGW	1 cut(s)	1785
Bsc4I	CCNNNNNNNGG	1 cut(s)	27
Bse118I	RCCGGY	1 cut(s)	1785
Bse3DI	GCAATG	2 cut(s)	1188, 2092
BseBI	CCWGG	1 cut(s)	489
BseCI	ATCGAT	1 cut(s)	450
BseDI	CCNNGG	4 cut(s)	180, 261, 657, 972
BseGI	GGATG	2 cut(s)	1075, 1816
BseLI	CCNNNNNNNGG	1 cut(s)	27
BseMI	GCAATG	2 cut(s)	1188, 2092
BseMII	CTCAG	1 cut(s)	255
BseRI	GAGGAG	1 cut(s)	28
BseSI	GKGCMC	1 cut(s)	721
BseXI	GCAGC	1 cut(s)	2125
Bsh1236I	CGCG	1 cut(s)	182
BshFI	GGCC	3 cut(s)	185, 656, 1397
BshTI	ACCGGT	1 cut(s)	1785
BshVI	ATCGAT	1 cut(s)	450
BsiHKAI	GWGCWC	1 cut(s)	91
BsiSI	CCGG	2 cut(s)	1786, 2120
BslFI	GGGAC	2 cut(s)	1164, 1275
BslI	CCNNNNNNNGG	1 cut(s)	27
BsmAI	GTCTC	3 cut(s)	1405, 1663, 2051
BsmFI	GGGAC	2 cut(s)	1164, 1275
BsmI	GAATGC	2 cut(s)	236, 492
BsnI	GGCC	3 cut(s)	185, 656, 1397
Bsp119I	TTCGAA	1 cut(s)	425
Bsp1286I	GDGCHC	3 cut(s)	91, 721, 1806
Bsp143I	GATC	2 cut(s)	106, 1083
Bsp19I	CCATGG	2 cut(s)	657, 972
BspACI	CCGC	3 cut(s)	160, 180, 182
BspANI	GGCC	3 cut(s)	185, 656, 1397
BspCNI	CTCAG	1 cut(s)	254
BspDI	ATCGAT	1 cut(s)	450
BspFNI	CGCG	1 cut(s)	182
BspHI	TCATGA	2 cut(s)	996, 1461
BspLI	GGNNCC	1 cut(s)	102
BspMI	ACCTGC	1 cut(s)	2090
BspPI	GGATC	1 cut(s)	101
BspQI	GCTCTTC	1 cut(s)	38
BspT104I	TTCGAA	1 cut(s)	425
BspTI	CTTAAG	1 cut(s)	2126
BsrDI	GCAATG	2 cut(s)	1188, 2092
BsrFI	RCCGGY	1 cut(s)	1785
BssAI	RCCGGY	1 cut(s)	1785
BssECI	CCNNGG	4 cut(s)	180, 261, 657, 972
BssMI	GATC	2 cut(s)	106, 1083
BssSI	CACGAG	1 cut(s)	1845
BssT1I	CCWWGG	3 cut(s)	261, 657, 972
Bst2BI	CACGAG	1 cut(s)	1845
Bst2UI	CCWGG	1 cut(s)	489
Bst4CI	ACNGT	5 cut(s)	771, 794, 871, 1266, 1549
Bst6I	CTCTTC	1 cut(s)	38
BstAFI	CTTAAG	1 cut(s)	2126
BstAPI	GCANNNNNTGC	1 cut(s)	2082
BstBAI	YACGTR	1 cut(s)	2135
BstBI	TTCGAA	1 cut(s)	425
BstC8I	GCNNGC	4 cut(s)	164, 187, 1605, 1996
BstDEI	CTNAG	3 cut(s)	241, 252, 1087
BstDSI	CCRYGG	3 cut(s)	180, 657, 972
BstF5I	GGATG	2 cut(s)	1075, 1816
BstFNI	CGCG	1 cut(s)	182
BstKTI	GATC	2 cut(s)	109, 1086
BstMAI	GTCTC	3 cut(s)	1405, 1663, 2051
BstMBI	GATC	2 cut(s)	106, 1083
BstMWI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
BstNI	CCWGG	1 cut(s)	489
BstNSI	RCATGY	2 cut(s)	1418, 1735
BstSCI	CCNGG	2 cut(s)	487, 2118
BstSFI	CTRYAG	2 cut(s)	1373, 1894
BstSLI	GKGCMC	1 cut(s)	721
BstUI	CGCG	1 cut(s)	182
BstV1I	GCAGC	1 cut(s)	2125
BstV2I	GAAGAC	2 cut(s)	478, 1734
BstX2I	RGATCY	2 cut(s)	106, 1083
BstYI	RGATCY	2 cut(s)	106, 1083
Bsu15I	ATCGAT	1 cut(s)	450
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	3 cut(s)	185, 656, 1397
BsuTUI	ATCGAT	1 cut(s)	450
BtgI	CCRYGG	3 cut(s)	180, 657, 972
BtsCI	GGATG	2 cut(s)	1075, 1816
BtsI	GCAGTG	3 cut(s)	41, 558, 1425
BtsIMutI	CAGTG	4 cut(s)	41, 558, 867, 1425
BveI	ACCTGC	1 cut(s)	2090
Cac8I	GCNNGC	4 cut(s)	164, 187, 1605, 1996
CaiI	CAGNNNCTG	1 cut(s)	587
CciI	TCATGA	2 cut(s)	996, 1461
Cfr10I	RCCGGY	1 cut(s)	1785
Cfr13I	GGNCC	1 cut(s)	100
Cfr42I	CCGCGG	1 cut(s)	183
ClaI	ATCGAT	1 cut(s)	450
Csp6I	GTAC	2 cut(s)	607, 1344
CspAI	ACCGGT	1 cut(s)	1785
CviAII	CATG	9 cut(s)	143, 344, 658, 973, 997, 1249, 1415, 1462, 1732
CviQI	GTAC	2 cut(s)	607, 1344
DdeI	CTNAG	3 cut(s)	241, 252, 1087
DpnI	GATC	2 cut(s)	108, 1085
DpnII	GATC	2 cut(s)	106, 1083
DraI	TTTAAA	1 cut(s)	1080
DraIII	CACNNNGTG	1 cut(s)	896
Eam1104I	CTCTTC	1 cut(s)	38
EarI	CTCTTC	1 cut(s)	38
Ecl136II	GAGCTC	1 cut(s)	89
Eco130I	CCWWGG	3 cut(s)	261, 657, 972
Eco24I	GRGCYC	2 cut(s)	91, 1806
Eco47I	GGWCC	1 cut(s)	100
Eco53kI	GAGCTC	1 cut(s)	89
Eco57I	CTGAAG	1 cut(s)	153
EcoICRI	GAGCTC	1 cut(s)	89
EcoO109I	RGGNCCY	1 cut(s)	100
EcoRI	GAATTC	2 cut(s)	1403, 1683
EcoRII	CCWGG	1 cut(s)	487
EcoT14I	CCWWGG	3 cut(s)	261, 657, 972
EcoT38I	GRGCYC	2 cut(s)	91, 1806
ErhI	CCWWGG	3 cut(s)	261, 657, 972
FaeI	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	2 cut(s)	1164, 1275
FatI	CATG	9 cut(s)	142, 343, 657, 972, 996, 1248, 1414, 1461, 1731
Fnu4HI	GCNGC	3 cut(s)	180, 183, 2139
FokI	GGATG	2 cut(s)	1082, 1823
FriOI	GRGCYC	2 cut(s)	91, 1806
Fsp4HI	GCNGC	3 cut(s)	180, 183, 2139
FspBI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
GluI	GCNGC	3 cut(s)	180, 183, 2139
GsuI	CTGGAG	2 cut(s)	1429, 1716
HaeIII	GGCC	3 cut(s)	185, 656, 1397
HapII	CCGG	2 cut(s)	1786, 2120
Hin1II	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
HincII	GTYRAC	2 cut(s)	439, 1927
HindII	GTYRAC	2 cut(s)	439, 1927
HindIII	AAGCTT	1 cut(s)	280
HinfI	GANTC	3 cut(s)	400, 584, 1877
HpaI	GTTAAC	1 cut(s)	1927
HpaII	CCGG	2 cut(s)	1786, 2120
HphI	GGTGA	1 cut(s)	1919
Hpy166II	GTNNAC	5 cut(s)	439, 635, 1423, 1783, 1927
Hpy188I	TCNGA	5 cut(s)	583, 589, 1545, 1941, 2014
Hpy188III	TCNNGA	8 cut(s)	64, 997, 1283, 1408, 1462, 1622, 1754, 1964
Hpy8I	GTNNAC	5 cut(s)	439, 635, 1423, 1783, 1927
HpyAV	CCTTC	5 cut(s)	177, 994, 1162, 1268, 1373
HpyCH4III	ACNGT	5 cut(s)	771, 794, 871, 1266, 1549
HpyCH4IV	ACGT	3 cut(s)	605, 1032, 2134
HpyF10VI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
HpyF3I	CTNAG	3 cut(s)	241, 252, 1087
HpySE526I	ACGT	3 cut(s)	605, 1032, 2134
Hsp92II	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
KspAI	GTTAAC	1 cut(s)	1927
KspI	CCGCGG	1 cut(s)	183
Kzo9I	GATC	2 cut(s)	106, 1083
LguI	GCTCTTC	1 cut(s)	38
Lsp1109I	GCAGC	1 cut(s)	2125
LweI	GCATC	3 cut(s)	901, 1063, 1358
MaeI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
MaeII	ACGT	3 cut(s)	605, 1032, 2134
MaeIII	GTNAC	7 cut(s)	481, 601, 811, 865, 916, 1021, 2130
MalI	GATC	2 cut(s)	108, 1085
MboI	GATC	2 cut(s)	106, 1083
MboII	GAAGA	5 cut(s)	25, 483, 1739, 1846, 1928
MfeI	CAATTG	1 cut(s)	80
MflI	RGATCY	2 cut(s)	106, 1083
MhlI	GDGCHC	3 cut(s)	91, 721, 1806
MmeI	TCCRAC	2 cut(s)	180, 1568
MroXI	GAANNNNTTC	1 cut(s)	1190
MslI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
MspA1I	CMGCKG	1 cut(s)	182
MspCI	CTTAAG	1 cut(s)	2126
MspI	CCGG	2 cut(s)	1786, 2120
MspR9I	CCNGG	2 cut(s)	489, 2120
MunI	CAATTG	1 cut(s)	80
Mva1269I	GAATGC	2 cut(s)	236, 492
MvaI	CCWGG	1 cut(s)	489
MvnI	CGCG	1 cut(s)	182
MwoI	GCNNNNNNNGC	3 cut(s)	968, 1394, 2082
NciI	CCSGG	1 cut(s)	2120
NcoI	CCATGG	2 cut(s)	657, 972
NdeII	GATC	2 cut(s)	106, 1083
NlaIII	CATG	9 cut(s)	146, 347, 661, 976, 1000, 1252, 1418, 1465, 1735
NlaIV	GGNNCC	1 cut(s)	102
NmuCI	GTSAC	1 cut(s)	865
NspI	RCATGY	2 cut(s)	1418, 1735
NspV	TTCGAA	1 cut(s)	425
PagI	TCATGA	2 cut(s)	996, 1461
PciI	ACATGT	1 cut(s)	1731
PciSI	GCTCTTC	1 cut(s)	38
PctI	GAATGC	2 cut(s)	236, 492
PdmI	GAANNNNTTC	1 cut(s)	1190
PfeI	GAWTC	3 cut(s)	400, 584, 1877
PinAI	ACCGGT	1 cut(s)	1785
PkrI	GCNGC	3 cut(s)	181, 184, 2140
Ppu21I	YACGTR	1 cut(s)	2135
PpuMI	RGGWCCY	1 cut(s)	100
PscI	ACATGT	1 cut(s)	1731
PshBI	ATTAAT	3 cut(s)	621, 831, 1467
PsiI	TTATAA	3 cut(s)	504, 725, 1350
Psp124BI	GAGCTC	1 cut(s)	91
Psp1406I	AACGTT	1 cut(s)	1032
Psp5II	RGGWCCY	1 cut(s)	100
Psp6I	CCWGG	1 cut(s)	487
PspGI	CCWGG	1 cut(s)	487
PspN4I	GGNNCC	1 cut(s)	102
PspPI	GGNCC	1 cut(s)	100
PspPPI	RGGWCCY	1 cut(s)	100
PsrI	GAACNNNNNNTAC	1 cut(s)	2115
PstNI	CAGNNNCTG	1 cut(s)	587
PsuI	RGATCY	2 cut(s)	106, 1083
RsaI	GTAC	2 cut(s)	608, 1345
RsaNI	GTAC	2 cut(s)	607, 1344
RseI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
SacI	GAGCTC	1 cut(s)	91
SacII	CCGCGG	1 cut(s)	183
SapI	GCTCTTC	1 cut(s)	38
SatI	GCNGC	3 cut(s)	180, 183, 2139
Sau3AI	GATC	2 cut(s)	106, 1083
Sau96I	GGNCC	1 cut(s)	100
ScrFI	CCNGG	2 cut(s)	489, 2120
SduI	GDGCHC	3 cut(s)	91, 721, 1806
SfaNI	GCATC	3 cut(s)	901, 1063, 1358
SfcI	CTRYAG	2 cut(s)	1373, 1894
Sfr303I	CCGCGG	1 cut(s)	183
SfuI	TTCGAA	1 cut(s)	425
SgrBI	CCGCGG	1 cut(s)	183
SinI	GGWCC	1 cut(s)	100
SmiMI	CAYNNNNRTG	3 cut(s)	872, 989, 1579
SmlI	CTYRAG	2 cut(s)	211, 2126
SmoI	CTYRAG	2 cut(s)	211, 2126
SsiI	CCGC	3 cut(s)	160, 180, 182
SspMI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818
SstI	GAGCTC	1 cut(s)	91
StyD4I	CCNGG	2 cut(s)	487, 2118
StyI	CCWWGG	3 cut(s)	261, 657, 972
TaaI	ACNGT	5 cut(s)	771, 794, 871, 1266, 1549
TaiI	ACGT	3 cut(s)	608, 1035, 2137
TaqI	TCGA	6 cut(s)	65, 131, 218, 425, 450, 774
TatI	WGTACW	1 cut(s)	1343
TauI	GCSGC	2 cut(s)	182, 185
TfiI	GAWTC	3 cut(s)	400, 584, 1877
TscAI	CASTG	4 cut(s)	48, 558, 874, 1425
TseFI	GTSAC	1 cut(s)	865
TseI	GCWGC	1 cut(s)	2138
Tsp45I	GTSAC	1 cut(s)	865
TspGWI	ACGGA	2 cut(s)	314, 1695
TspRI	CASTG	4 cut(s)	48, 558, 874, 1425
Vha464I	CTTAAG	1 cut(s)	2126
VpaK11BI	GGWCC	1 cut(s)	100
VspI	ATTAAT	3 cut(s)	621, 831, 1467
XapI	RAATTY	4 cut(s)	114, 335, 1403, 1683
XceI	RCATGY	2 cut(s)	1418, 1735
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XmnI	GAANNNNTTC	1 cut(s)	1190
XspI	CTAG	5 cut(s)	233, 1608, 1650, 1724, 1818

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.