Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001535

Physical Location & Seq

Genomic Coordinates Forward (+)

46268927 .. 46272013

Gene Structure 3087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ32662

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGCGGTCATTACAGGTTTCCAAGACACGGCTCTTCTCCTCACTGCCATCTATTACTCCCCTATCTGATGTAGAAACCCAATTGAGAGCTTTTTGGAAGGACCTGGATCCTAAAATTTCGGAGGCGCTTTCGCTTTTTCATCATGCCATACATTCCAACCGCCTGCCTCCGGGGTCAGCCTCAGCCTGCAACTTCCTTGTAGACACTCTCGCAAGGTCAAAAAATTATGAACTATCATTCTCAGTTTATACCAAGATGACCAAGGTTGGTATTATTCCAAGCTTTATATCATTGAGTTGTTTAGTTTCATATTTTGTCAATACGCGTAAGCTAGAATTCGCACCCGGGGTTTTCGGCTTGGTATTAAAGCGTGGATTTCAGTTAAATGAGTATGTTATGAATCTTGCATTAAATGGTTTTTGTTCTAATGATGAGGTTGACAAGGCAATTGAGTTGTTTAGTGTAATGAGAAGACATTTTGTAAAGCCTGGTGTTCGTAGTTATAACATTCTTATAAATGGACTTTGCAAAGCGAAAAAGTTGAAAGAAGCAGTGGAATTGTTGGTTGATATGGAGGTGTCGGATTCTGAACCGAATGTGGTAACATACAGCACATTGATTAATGGATTTTGTAAACAGGGTAGAGTGGATGAGGCCATGGGTTTCTTGGAGGAGATGAGCCAAAAGGGTTTGGAAATGGATGTTTTTGTATATGGGGCCCTTATAAGTGGTTTTTGTGCTAAAGGGAGTTTTGATAGAGGAAAACAACTTTTTGATGAGATGTTAAACAAGGGCATTTCTCCTAATGTGGTTACGTATAGTTGTTTGATTAATTATCTTTCAAAGACAGGTAAGTGGAAAGAAGTCACTGTGATGGTAAATGATATGATACAATGTGGAGTTCCCCCTGATGCTATTACTTACACAGGTATCCTTGATGGGCTTTTCAAAAATGGAATGGCTACAAAAGCCATGGCGATATTCAATTTAATGCTATTGAAGGGTACAGAGCCTAATAATGTAACATACAACGTTATGATTGATGGATTGTGCAAGGAGGGGTTGGTGGATGATGCTTTAAAGATCTTAGAAATGATGAAAGGGAAAGGTGTAAAGCCTGATGTAATCACTTACAACACATTACTAATGGGACTATCCTGTGATGGGAAGGTTGATGAGATGATGCAACTTTTCAGTAAGATAACAAATGATGAGAACTTTGTTGAGCCAGATGTTATGACGTATAACATGCTCATTTTGGGACTGTGCAAGGAAGGTAATCTTGATCAGGCTATGGAGATGTATCATACAATGGTTGAAAGGGGCATTTCTGGTAGCTTATGTACTTATAACACTTTGATCGACAGATGCATACAGGAAGGATTAGTTGACAAGGCCATAGAATTCTGGAGACATGCAGTTGACTTGGGAGTTATACCTAACTCAATCACTTATGGTGTCTTGATCAATGGATTTTGCAAAAATCAAATGGTCAGGCTTGCAAAAGGACTTTTTAGTAAAATGAGAGCTTCTGGGATTAGTCCTACTGTAATTGACTACAACACTTTGATGTCATCGTTGTGCAAGGAGGGTAGTTTGGAGCAAGCTAGGATGTTGTTTCAAGAAATGAGAACTGTAAATCATTCACCTTGTGTTGTCTCATTTAATACCATAATTGACGGACTTCTTAAAGCTGGAGATATTAAATCTACTAAAGAGCTGCTAGAGGATATGTTCAAATTGGGTTTAATTCCCGATAGAATTACTTTCTCTACATTAGTGAACCGGTTTTCAAAACTTGGGCTATTGGCTGAGGCTAGAATGTGCATTGAGAAGATGATTGCTCGTGGTATTGAACCTGATGTCTTTGTATTCGATTCTTTATTAAAAGGCTACAGTTCTAAAGGTGAAACAGAAGAAATTGTTAATTTGCTTCATCAGATGGCAGAAAAGGGTGTTGTTCTTGACAAAGATATCACTTCTACCATCTTGGCGTGCCTCTGTCAAATCTCTGATGATGTTGATGTGATGGAGGTTCTCCCTACTTTTTCCAAAGACATATCGAAAGGAATAAGCATTACCTGCAATGAGCTATTGATGAAAGTCAATAAATTTTATCCGGAACTAAAGTTATGTGCTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

714

Amino Acids

79.48

Weight (kDa)

5.83

Isoelectric Point (pI)

24.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	112 - 223	2.1e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	129 - 177	2.6e-12	PPR repeat family
PPR_1	PF12854	161 - 192	1.2e-10	PPR repeat
PPR_2	PF13041	163 - 212	2.6e-17	PPR repeat family
PPR	PF01535	167 - 193	1.3e-06	PPR repeat
PPR_1	PF12854	197 - 227	3.9e-16	PPR repeat
PPR_2	PF13041	198 - 246	1.1e-16	PPR repeat family
PPR	PF01535	201 - 231	1.2e-09	PPR repeat
PPR_3	PF13812	223 - 280	9.7e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	229 - 261	3.4e-08	PPR repeat
PPR_2	PF13041	234 - 281	4.4e-14	PPR repeat family
PPR	PF01535	237 - 266	7.8e-06	PPR repeat
PPR_long	PF17177	250 - 327	5.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	268 - 317	6.2e-14	PPR repeat family
PPR_3	PF13812	293 - 348	5.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	300 - 331	7.5e-06	PPR repeat
PPR_2	PF13041	306 - 352	3.2e-14	PPR repeat family
TPR_24	PF23276	317 - 437	3.4e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	334 - 367	3.9e-13	PPR repeat
PPR_2	PF13041	341 - 385	1.6e-16	PPR repeat family
PPR	PF01535	341 - 371	3.2e-10	PPR repeat
PPR_3	PF13812	361 - 419	2.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	369 - 400	2.6e-08	PPR repeat
PPR_3	PF13812	408 - 457	8.4e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	408 - 439	4.3e-11	PPR repeat
PPR_2	PF13041	410 - 457	2.2e-14	PPR repeat family
PPR	PF01535	414 - 443	4.2e-07	PPR repeat
PPR_long	PF17177	424 - 546	2.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	448 - 494	1.1e-12	PPR repeat family
PPR_3	PF13812	472 - 526	2.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	477 - 509	3e-08	PPR repeat
PPR_2	PF13041	480 - 517	4.9e-08	PPR repeat family
PPR	PF01535	483 - 513	3.8e-06	PPR repeat
PPR_3	PF13812	504 - 560	4.3e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	512 - 544	1.2e-09	PPR repeat
PPR_2	PF13041	515 - 550	1.4e-06	PPR repeat family
PPR	PF01535	520 - 545	3.6e-06	PPR repeat
PPR_2	PF13041	550 - 599	4.6e-12	PPR repeat family
PPR_2	PF13041	620 - 668	7.7e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28010
fragaria_vesca	FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_3g35120 FvH4_5g33200 FvH4_5g33200 FvH4_5g33200
malus_domestica	MD08G1215500.v1.1 MD15G1399800.v1.1 MD15G1400000.v1.1
prunus_persica	Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1 Prupe.1G547200_v2.0.a1
pyrus_communis	pycom08g18660 pycom15g35860
rosa_chinensis	RchiOBHm_Chr5g0048941 RchiOBHm_Chr7g0231581
rosa_laevigata	RLG00000001411 RLG00000001412 RLG00000034570
rosa_multiflora	Rmu_sc0007946.1_g000003
rosa_roxburghii	Rroxscaffold_1G00032970 Rroxscaffold_3G00229080
rosa_rugosa	Rorug05G0241400 Rorug07G0267200 Rorug07G0267300
rosa_samantha	Rh5AG321400 Rh5CG357100 Rh5DG343500 Rh7AG421600 Rh7AG421700 Rh7AG421900 Rh7BG396300 Rh7CG441900 Rh7CG442200 Rh7DG414300
rosa_wichuraiana	Rw5G030330 Rw7G034790 Rw7G034880 Rw7G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	504, 515, 725, 1350
Acc36I	ACCTGC	1 cut(s)	2090
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	1 cut(s)	325
AccIII	TCCGGA	1 cut(s)	2119
AciI	CCGC	2 cut(s)	4, 160
AclI	AACGTT	1 cut(s)	1032
AclWI	GGATC	2 cut(s)	101, 114
AcsI	RAATTY	4 cut(s)	114, 335, 1403, 2111
AdeI	CACNNNGTG	1 cut(s)	1652
AfaI	GTAC	2 cut(s)	1006, 1345
AfiI	CCNNNNNNNGG	3 cut(s)	27, 169, 1164
AflIII	ACRYGT	1 cut(s)	323
AgeI	ACCGGT	1 cut(s)	1785
AjnI	CCWGG	2 cut(s)	102, 487
AluBI	AGCT	9 cut(s)	89, 282, 331, 1338, 1529, 1607, 1694, 1720, 2092
AluI	AGCT	9 cut(s)	89, 282, 331, 1338, 1529, 1607, 1694, 1720, 2092
Alw26I	GTCTC	2 cut(s)	1405, 1663
AlwI	GGATC	2 cut(s)	101, 114
Ama87I	CYCGRG	1 cut(s)	344
Aor13HI	TCCGGA	1 cut(s)	2119
AoxI	GGCC	3 cut(s)	654, 717, 1395
ApaI	GGGCCC	1 cut(s)	721
ApeKI	GCWGC	2 cut(s)	1720, 2138
ApoI	RAATTY	4 cut(s)	114, 335, 1403, 2111
AseI	ATTAAT	2 cut(s)	621, 831
AsiGI	ACCGGT	1 cut(s)	1785
AspLEI	GCGC	1 cut(s)	127
AspS9I	GGNCC	3 cut(s)	100, 717, 718
AsuC2I	CCSGG	3 cut(s)	171, 345, 346
AsuHPI	GGTGA	2 cut(s)	1638, 1919
AvaI	CYCGRG	1 cut(s)	344
AvaII	GGWCC	1 cut(s)	100
BaeGI	GKGCMC	1 cut(s)	721
BaeI	ACNNNNGTAYC	2 cut(s)	913, 946
BamHI	GGATCC	1 cut(s)	106
BanII	GRGCYC	1 cut(s)	721
BauI	CACGAG	1 cut(s)	1845
BbsI	GAAGAC	1 cut(s)	478
BbvCI	CCTCAGC	2 cut(s)	181, 1812
BbvI	GCAGC	2 cut(s)	1707, 2125
BccI	CCATC	8 cut(s)	55, 868, 932, 1037, 1157, 1936, 1994, 2023
BceAI	ACGGC	1 cut(s)	44
BciT130I	CCWGG	2 cut(s)	104, 489
BciVI	GTATCC	1 cut(s)	941
BclI	TGATCA	2 cut(s)	1285, 1464
BcnI	CCSGG	3 cut(s)	171, 345, 346
BcoDI	GTCTC	2 cut(s)	1405, 1663
BfaI	CTAG	4 cut(s)	332, 1608, 1724, 1818
BfmI	CTRYAG	1 cut(s)	1894
BfoI	RGCGCY	1 cut(s)	128
BfuAI	ACCTGC	1 cut(s)	2090
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	1 cut(s)	1083
BisI	GCNGC	2 cut(s)	1721, 2139
BlsI	GCNGC	2 cut(s)	1722, 2140
Bme1390I	CCNGG	5 cut(s)	104, 171, 345, 346, 489
Bme18I	GGWCC	1 cut(s)	100
BmeT110I	CYCGRG	1 cut(s)	344
BmgT120I	GGNCC	3 cut(s)	100, 717, 718
BmiI	GGNNCC	3 cut(s)	108, 718, 719
BmrFI	CCNGG	5 cut(s)	104, 171, 345, 346, 489
BmsI	GCATC	4 cut(s)	901, 1063, 1173, 1358
BpiI	GAAGAC	1 cut(s)	478
BpmI	CTGGAG	2 cut(s)	1429, 1716
Bpu10I	CCTNAGC	2 cut(s)	181, 1812
BpuMI	CCSGG	3 cut(s)	171, 345, 346
BsaAI	YACGTR	1 cut(s)	816
BsaJI	CCNNGG	6 cut(s)	170, 261, 344, 345, 657, 972
BsaWI	WCCGGW	2 cut(s)	1785, 2119
Bsc4I	CCNNNNNNNGG	3 cut(s)	27, 169, 1164
Bse118I	RCCGGY	1 cut(s)	1785
Bse3DI	GCAATG	1 cut(s)	2092
BseAI	TCCGGA	1 cut(s)	2119
BseBI	CCWGG	2 cut(s)	104, 489
BseDI	CCNNGG	6 cut(s)	170, 261, 344, 345, 657, 972
BseGI	GGATG	4 cut(s)	655, 706, 1075, 1617
BseLI	CCNNNNNNNGG	3 cut(s)	27, 169, 1164
BseMI	GCAATG	1 cut(s)	2092
BseMII	CTCAG	3 cut(s)	195, 255, 1803
BseRI	GAGGAG	2 cut(s)	28, 686
BseSI	GKGCMC	1 cut(s)	721
BseXI	GCAGC	2 cut(s)	1707, 2125
Bsh1236I	CGCG	1 cut(s)	325
BshFI	GGCC	3 cut(s)	656, 719, 1397
BshTI	ACCGGT	1 cut(s)	1785
BsiHKCI	CYCGRG	1 cut(s)	344
BsiSI	CCGG	4 cut(s)	170, 345, 1786, 2120
BslFI	GGGAC	2 cut(s)	1164, 1275
BslI	CCNNNNNNNGG	3 cut(s)	27, 169, 1164
BsmAI	GTCTC	2 cut(s)	1405, 1663
BsmFI	GGGAC	2 cut(s)	1164, 1275
BsnI	GGCC	3 cut(s)	656, 719, 1397
BsoBI	CYCGRG	1 cut(s)	344
Bsp120I	GGGCCC	1 cut(s)	717
Bsp1286I	GDGCHC	1 cut(s)	721
Bsp13I	TCCGGA	1 cut(s)	2119
Bsp143I	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
Bsp19I	CCATGG	2 cut(s)	657, 972
BspACI	CCGC	2 cut(s)	4, 160
BspANI	GGCC	3 cut(s)	656, 719, 1397
BspCNI	CTCAG	3 cut(s)	194, 254, 1804
BspEI	TCCGGA	1 cut(s)	2119
BspFNI	CGCG	1 cut(s)	325
BspLI	GGNNCC	3 cut(s)	108, 718, 719
BspMI	ACCTGC	1 cut(s)	2090
BspPI	GGATC	2 cut(s)	101, 114
BspQI	GCTCTTC	1 cut(s)	38
BsrDI	GCAATG	1 cut(s)	2092
BsrFI	RCCGGY	1 cut(s)	1785
BssAI	RCCGGY	1 cut(s)	1785
BssECI	CCNNGG	6 cut(s)	170, 261, 344, 345, 657, 972
BssMI	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
BssSI	CACGAG	1 cut(s)	1845
BssT1I	CCWWGG	3 cut(s)	261, 657, 972
Bst2BI	CACGAG	1 cut(s)	1845
Bst2UI	CCWGG	2 cut(s)	104, 489
Bst4CI	ACNGT	5 cut(s)	871, 1266, 1549, 1636, 1898
Bst6I	CTCTTC	1 cut(s)	38
BstAPI	GCANNNNNTGC	1 cut(s)	2082
BstBAI	YACGTR	1 cut(s)	816
BstC8I	GCNNGC	5 cut(s)	164, 187, 1500, 1605, 1996
BstDEI	CTNAG	4 cut(s)	181, 241, 1087, 1812
BstDSI	CCRYGG	2 cut(s)	657, 972
BstF5I	GGATG	4 cut(s)	655, 706, 1075, 1617
BstFNI	CGCG	1 cut(s)	325
BstH2I	RGCGCY	1 cut(s)	128
BstHHI	GCGC	1 cut(s)	127
BstKTI	GATC	5 cut(s)	109, 1086, 1288, 1362, 1467
BstMAI	GTCTC	2 cut(s)	1405, 1663
BstMBI	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
BstMWI	GCNNNNNNNGC	2 cut(s)	968, 2082
BstNI	CCWGG	2 cut(s)	104, 489
BstNSI	RCATGY	2 cut(s)	1252, 1418
BstSCI	CCNGG	5 cut(s)	102, 169, 343, 344, 487
BstSFI	CTRYAG	1 cut(s)	1894
BstSLI	GKGCMC	1 cut(s)	721
BstSNI	TACGTA	1 cut(s)	816
BstUI	CGCG	1 cut(s)	325
BstV1I	GCAGC	2 cut(s)	1707, 2125
BstV2I	GAAGAC	1 cut(s)	478
BstX2I	RGATCY	2 cut(s)	106, 1083
BstYI	RGATCY	2 cut(s)	106, 1083
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	3 cut(s)	656, 719, 1397
BtgI	CCRYGG	2 cut(s)	657, 972
BtsCI	GGATG	4 cut(s)	655, 706, 1075, 1617
BtsI	GCAGTG	2 cut(s)	41, 558
BtsIMutI	CAGTG	3 cut(s)	41, 558, 867
BveI	ACCTGC	1 cut(s)	2090
Cac8I	GCNNGC	5 cut(s)	164, 187, 1500, 1605, 1996
CfoI	GCGC	1 cut(s)	127
Cfr10I	RCCGGY	1 cut(s)	1785
Cfr13I	GGNCC	3 cut(s)	100, 717, 718
Cfr9I	CCCGGG	1 cut(s)	344
Csp6I	GTAC	2 cut(s)	1005, 1344
CspAI	ACCGGT	1 cut(s)	1785
CviAII	CATG	5 cut(s)	143, 658, 973, 1249, 1415
CviQI	GTAC	2 cut(s)	1005, 1344
DdeI	CTNAG	4 cut(s)	181, 241, 1087, 1812
DpnI	GATC	5 cut(s)	108, 1085, 1287, 1361, 1466
DpnII	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
DraI	TTTAAA	1 cut(s)	1080
DraIII	CACNNNGTG	1 cut(s)	1652
Eam1104I	CTCTTC	1 cut(s)	38
EarI	CTCTTC	1 cut(s)	38
Eco105I	TACGTA	1 cut(s)	816
Eco130I	CCWWGG	3 cut(s)	261, 657, 972
Eco24I	GRGCYC	1 cut(s)	721
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	1 cut(s)	100
Eco88I	CYCGRG	1 cut(s)	344
EcoO109I	RGGNCCY	3 cut(s)	100, 717, 718
EcoRI	GAATTC	2 cut(s)	335, 1403
EcoRII	CCWGG	2 cut(s)	102, 487
EcoRV	GATATC	1 cut(s)	1975
EcoT14I	CCWWGG	3 cut(s)	261, 657, 972
EcoT22I	ATGCAT	1 cut(s)	1373
EcoT38I	GRGCYC	1 cut(s)	721
ErhI	CCWWGG	3 cut(s)	261, 657, 972
FaeI	CATG	5 cut(s)	146, 661, 976, 1252, 1418
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	2 cut(s)	1164, 1275
FatI	CATG	5 cut(s)	142, 657, 972, 1248, 1414
FbaI	TGATCA	2 cut(s)	1285, 1464
FblI	GTMKAC	1 cut(s)	201
Fnu4HI	GCNGC	2 cut(s)	1721, 2139
FokI	GGATG	4 cut(s)	662, 713, 1082, 1624
FriOI	GRGCYC	1 cut(s)	721
Fsp4HI	GCNGC	2 cut(s)	1721, 2139
FspBI	CTAG	4 cut(s)	332, 1608, 1724, 1818
GlaI	GCGC	1 cut(s)	126
GluI	GCNGC	2 cut(s)	1721, 2139
GsuI	CTGGAG	2 cut(s)	1429, 1716
HaeII	RGCGCY	1 cut(s)	128
HaeIII	GGCC	3 cut(s)	656, 719, 1397
HapII	CCGG	4 cut(s)	170, 345, 1786, 2120
HhaI	GCGC	1 cut(s)	127
Hin1II	CATG	5 cut(s)	146, 661, 976, 1252, 1418
Hin6I	GCGC	1 cut(s)	125
HinP1I	GCGC	1 cut(s)	125
HincII	GTYRAC	3 cut(s)	439, 1390, 1423
HindII	GTYRAC	3 cut(s)	439, 1390, 1423
HindIII	AAGCTT	1 cut(s)	280
HinfI	GANTC	3 cut(s)	400, 584, 1877
HpaII	CCGG	4 cut(s)	170, 345, 1786, 2120
HphI	GGTGA	2 cut(s)	1638, 1919
Hpy166II	GTNNAC	6 cut(s)	202, 439, 635, 1390, 1423, 1783
Hpy188I	TCNGA	6 cut(s)	67, 121, 583, 589, 1941, 2014
Hpy188III	TCNNGA	7 cut(s)	1283, 1408, 1462, 1622, 1754, 1964, 2120
Hpy8I	GTNNAC	6 cut(s)	202, 439, 635, 1390, 1423, 1783
HpyAV	CCTTC	5 cut(s)	91, 994, 1162, 1268, 1373
HpyCH4III	ACNGT	5 cut(s)	871, 1266, 1549, 1636, 1898
HpyCH4IV	ACGT	3 cut(s)	815, 1032, 1241
HpyF10VI	GCNNNNNNNGC	2 cut(s)	968, 2082
HpyF3I	CTNAG	4 cut(s)	181, 241, 1087, 1812
HpySE526I	ACGT	3 cut(s)	815, 1032, 1241
Hsp92II	CATG	5 cut(s)	146, 661, 976, 1252, 1418
HspAI	GCGC	1 cut(s)	125
Kpn2I	TCCGGA	1 cut(s)	2119
Ksp22I	TGATCA	2 cut(s)	1285, 1464
Kzo9I	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
LguI	GCTCTTC	1 cut(s)	38
LmnI	GCTCC	1 cut(s)	1600
Lsp1109I	GCAGC	2 cut(s)	1707, 2125
LweI	GCATC	4 cut(s)	901, 1063, 1173, 1358
MaeI	CTAG	4 cut(s)	332, 1608, 1724, 1818
MaeII	ACGT	3 cut(s)	815, 1032, 1241
MaeIII	GTNAC	4 cut(s)	601, 811, 865, 1021
MalI	GATC	5 cut(s)	108, 1085, 1287, 1361, 1466
MboI	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
MboII	GAAGA	4 cut(s)	25, 483, 1846, 1928
MfeI	CAATTG	2 cut(s)	80, 447
MflI	RGATCY	2 cut(s)	106, 1083
MhlI	GDGCHC	1 cut(s)	721
MluI	ACGCGT	1 cut(s)	323
MmeI	TCCRAC	2 cut(s)	180, 561
Mph1103I	ATGCAT	1 cut(s)	1373
MroI	TCCGGA	1 cut(s)	2119
MslI	CAYNNNNRTG	3 cut(s)	872, 1568, 1579
MspI	CCGG	4 cut(s)	170, 345, 1786, 2120
MspR9I	CCNGG	5 cut(s)	104, 171, 345, 346, 489
MunI	CAATTG	2 cut(s)	80, 447
MvaI	CCWGG	2 cut(s)	104, 489
MvnI	CGCG	1 cut(s)	325
MwoI	GCNNNNNNNGC	2 cut(s)	968, 2082
NciI	CCSGG	3 cut(s)	171, 345, 346
NcoI	CCATGG	2 cut(s)	657, 972
NdeII	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
NlaIII	CATG	5 cut(s)	146, 661, 976, 1252, 1418
NlaIV	GGNNCC	3 cut(s)	108, 718, 719
NmuCI	GTSAC	1 cut(s)	865
NsiI	ATGCAT	1 cut(s)	1373
NspI	RCATGY	2 cut(s)	1252, 1418
PciSI	GCTCTTC	1 cut(s)	38
PfeI	GAWTC	3 cut(s)	400, 584, 1877
PinAI	ACCGGT	1 cut(s)	1785
PkrI	GCNGC	2 cut(s)	1722, 2140
Ppu21I	YACGTR	1 cut(s)	816
PpuMI	RGGWCCY	1 cut(s)	100
PshBI	ATTAAT	2 cut(s)	621, 831
PsiI	TTATAA	4 cut(s)	504, 515, 725, 1350
Psp1406I	AACGTT	1 cut(s)	1032
Psp5II	RGGWCCY	1 cut(s)	100
Psp6I	CCWGG	2 cut(s)	102, 487
PspGI	CCWGG	2 cut(s)	102, 487
PspN4I	GGNNCC	3 cut(s)	108, 718, 719
PspOMI	GGGCCC	1 cut(s)	717
PspPI	GGNCC	3 cut(s)	100, 717, 718
PspPPI	RGGWCCY	1 cut(s)	100
PsuI	RGATCY	2 cut(s)	106, 1083
RsaI	GTAC	2 cut(s)	1006, 1345
RsaNI	GTAC	2 cut(s)	1005, 1344
RseI	CAYNNNNRTG	3 cut(s)	872, 1568, 1579
SapI	GCTCTTC	1 cut(s)	38
SatI	GCNGC	2 cut(s)	1721, 2139
Sau3AI	GATC	5 cut(s)	106, 1083, 1285, 1359, 1464
Sau96I	GGNCC	3 cut(s)	100, 717, 718
ScrFI	CCNGG	5 cut(s)	104, 171, 345, 346, 489
SduI	GDGCHC	1 cut(s)	721
SfaNI	GCATC	4 cut(s)	901, 1063, 1173, 1358
SfcI	CTRYAG	1 cut(s)	1894
SinI	GGWCC	1 cut(s)	100
SmaI	CCCGGG	1 cut(s)	346
SmiMI	CAYNNNNRTG	3 cut(s)	872, 1568, 1579
SnaBI	TACGTA	1 cut(s)	816
SsiI	CCGC	2 cut(s)	4, 160
SspMI	CTAG	4 cut(s)	332, 1608, 1724, 1818
StyD4I	CCNGG	5 cut(s)	102, 169, 343, 344, 487
StyI	CCWWGG	3 cut(s)	261, 657, 972
TaaI	ACNGT	5 cut(s)	871, 1266, 1549, 1636, 1898
TaiI	ACGT	3 cut(s)	818, 1035, 1244
TaqI	TCGA	3 cut(s)	1362, 1875, 2063
TatI	WGTACW	1 cut(s)	1343
TfiI	GAWTC	3 cut(s)	400, 584, 1877
TscAI	CASTG	3 cut(s)	48, 558, 874
TseFI	GTSAC	1 cut(s)	865
TseI	GCWGC	2 cut(s)	1720, 2138
Tsp45I	GTSAC	1 cut(s)	865
TspDTI	ATGAA	7 cut(s)	128, 243, 297, 413, 1112, 1925, 2114
TspGWI	ACGGA	1 cut(s)	1695
TspMI	CCCGGG	1 cut(s)	344
TspRI	CASTG	3 cut(s)	48, 558, 874
VpaK11BI	GGWCC	1 cut(s)	100
VspI	ATTAAT	2 cut(s)	621, 831
XapI	RAATTY	4 cut(s)	114, 335, 1403, 2111
XceI	RCATGY	2 cut(s)	1252, 1418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XmaI	CCCGGG	1 cut(s)	344
XmiI	GTMKAC	1 cut(s)	201
XspI	CTAG	4 cut(s)	332, 1608, 1724, 1818
Zsp2I	ATGCAT	1 cut(s)	1373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.