MD08G1216900.v1.1

Belongs to the SNF7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003138
Physical Location & Seq
Reverse (-)
27955903 .. 27959748
3846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1216900.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAAGAGGGTCTTTGGTGTCAAGAAAGACAAAGAGCCCCCTCCTTCTGTTGGCGATGCCTCCGATAGGATCAATAAAAGAGGCGAAACAGTCGATGAAAAGATCAAAAAGCTTGATATTGAACTGAATAGATACAAAGAGCAAATCAAGAAGACAAGGCCCGGTCCTGCACAAGAAGCTGTTAAGTCTCGAGCCATGAGGGTTCTCAAGCAGAAAAGAATGTATGAAGGACAACGGGACATGCTGTATAATCAGACATTTAACCTTGATCAAGTTTCCTTTGCTTCAGAGGGTATAAAAGATGCTCAGCAAACGATGACAGCTCTGAAGTCTGCAAACAAGGAGCTCAAGGGAATGATGAAAACCGTGAAGATTCAAGATATTGATAATTTGCAAGATGAAATGATGGACCTGATGGATGTGAGCAATGAAATTCAAGAGACCCTTGGCAGAAGCTATAGTGTTCCTGATGACATTGATGAGGAAGATCTTATGGGTGAGCTGGATGCTCTAGAAGCAGACATGGGTACGGAAACTGAAGCTGATGGGATTCCCTCCTATCTCCAACCTGATGCTGAACCGGACTTGGATGCGGAGCTCCGCTTGCCTTCAGCACCCACAGGACAGGCAACAGCTCCGGCTGGGAGATCCAATGCCCAGGCTGAGGACGAATTGGGCTTACCAGCTGTCCCTCGGGCAACACTTCGCGGTTAG

Protein Analysis

238

Amino Acids

26.36

Weight (kDa)

4.72

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 13 - 208 3.6e-47 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 708
AciI CCGC 3 cut(s) 593, 601, 708
AclWI GGATC 2 cut(s) 77, 642
AcsI RAATTY 1 cut(s) 432
AcuI CTGAAG 4 cut(s) 270, 347, 558, 594
AfaI GTAC 1 cut(s) 529
AfiI CCNNNNNNNGG 3 cut(s) 50, 66, 664
AgsI TTSAA 3 cut(s) 122, 377, 437
AjnI CCWGG 1 cut(s) 657
Alw21I GWGCWC 2 cut(s) 348, 600
Alw26I GTCTC 2 cut(s) 192, 434
AlwI GGATC 2 cut(s) 77, 642
Ama87I CYCGRG 2 cut(s) 189, 693
AoxI GGCC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 432
AspS9I GGNCC 3 cut(s) 159, 164, 409
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 509
AvaI CYCGRG 2 cut(s) 189, 693
AvaII GGWCC 2 cut(s) 164, 409
BanII GRGCYC 3 cut(s) 39, 348, 600
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 2 cut(s) 348, 600
BbvCI CCTCAGC 1 cut(s) 663
BccI CCATC 3 cut(s) 400, 409, 539
BciT130I CCWGG 1 cut(s) 659
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 192, 434
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 1 cut(s) 457
BglII AGATCT 1 cut(s) 487
BlpI GCTNAGC 1 cut(s) 306
Bme1390I CCNGG 2 cut(s) 162, 659
Bme18I GGWCC 2 cut(s) 164, 409
BmeT110I CYCGRG 2 cut(s) 189, 693
BmgT120I GGNCC 3 cut(s) 159, 164, 409
BmrFI CCNGG 2 cut(s) 162, 659
BmsI GCATC 5 cut(s) 46, 292, 496, 562, 580
BpiI GAAGAC 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 663
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 2 cut(s) 191, 332
BpuMI CCSGG 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 445, 657, 692
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 66, 664
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 659
BseDI CCNNGG 3 cut(s) 445, 657, 692
BseGI GGATG 3 cut(s) 424, 511, 595
BseLI CCNNNNNNNGG 3 cut(s) 50, 66, 664
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 2 cut(s) 320, 654
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 1 cut(s) 160
BsiHKAI GWGCWC 2 cut(s) 348, 600
BsiHKCI CYCGRG 2 cut(s) 189, 693
BsiSI CCGG 3 cut(s) 162, 581, 638
BslFI GGGAC 2 cut(s) 251, 674
BslI CCNNNNNNNGG 3 cut(s) 50, 66, 664
BsmAI GTCTC 2 cut(s) 192, 434
BsmFI GGGAC 2 cut(s) 251, 674
BsnI GGCC 1 cut(s) 160
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 2 cut(s) 189, 693
Bsp1286I GDGCHC 3 cut(s) 39, 348, 600
Bsp143I GATC 5 cut(s) 69, 102, 268, 487, 647
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 3 cut(s) 593, 601, 708
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 2 cut(s) 319, 655
BspFNI CGCG 1 cut(s) 708
BspPI GGATC 2 cut(s) 77, 642
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 445, 657, 692
BssMI GATC 5 cut(s) 69, 102, 268, 487, 647
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 2 cut(s) 91, 367
BstC8I GCNNGC 1 cut(s) 605
BstDEI CTNAG 2 cut(s) 306, 663
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 424, 511, 595
BstFNI CGCG 1 cut(s) 708
BstKTI GATC 5 cut(s) 72, 105, 271, 490, 650
BstMAI GTCTC 2 cut(s) 192, 434
BstMBI GATC 5 cut(s) 69, 102, 268, 487, 647
BstMWI GCNNNNNNNGC 3 cut(s) 176, 515, 604
BstNI CCWGG 1 cut(s) 659
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 160, 657
BstSFI CTRYAG 1 cut(s) 457
BstUI CGCG 1 cut(s) 708
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 2 cut(s) 487, 647
BstYI RGATCY 2 cut(s) 487, 647
BsuRI GGCC 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 3 cut(s) 424, 511, 595
Cac8I GCNNGC 1 cut(s) 605
Cfr13I GGNCC 3 cut(s) 159, 164, 409
Csp6I GTAC 1 cut(s) 528
CviAII CATG 3 cut(s) 196, 241, 523
CviQI GTAC 1 cut(s) 528
DdeI CTNAG 2 cut(s) 306, 663
DpnI GATC 5 cut(s) 71, 104, 270, 489, 649
DpnII GATC 5 cut(s) 69, 102, 268, 487, 647
Ecl136II GAGCTC 2 cut(s) 346, 598
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 3 cut(s) 39, 348, 600
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 164, 409
Eco53kI GAGCTC 2 cut(s) 346, 598
Eco57I CTGAAG 4 cut(s) 270, 347, 558, 594
Eco88I CYCGRG 2 cut(s) 189, 693
EcoICRI GAGCTC 2 cut(s) 346, 598
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 3 cut(s) 39, 348, 600
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 3 cut(s) 199, 244, 526
FaiI YATR 8 cut(s) 197, 225, 242, 249, 296, 459, 494, 524
FalI AAGNNNNNCTT 3 cut(s) 28, 429, 461
FaqI GGGAC 2 cut(s) 251, 674
FatI CATG 3 cut(s) 195, 240, 522
FbaI TGATCA 1 cut(s) 268
FokI GGATG 3 cut(s) 431, 518, 602
FriOI GRGCYC 3 cut(s) 39, 348, 600
FspBI CTAG 1 cut(s) 512
GsaI CCCAGC 1 cut(s) 645
HaeIII GGCC 1 cut(s) 160
HapII CCGG 3 cut(s) 162, 581, 638
Hin1II CATG 3 cut(s) 199, 244, 526
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 373, 550
HpaII CCGG 3 cut(s) 162, 581, 638
HphI GGTGA 1 cut(s) 509
Hpy188I TCNGA 4 cut(s) 64, 255, 289, 327
Hpy188III TCNNGA 7 cut(s) 22, 148, 189, 377, 437, 467, 512
HpyAV CCTTC 3 cut(s) 54, 221, 618
HpyCH4III ACNGT 2 cut(s) 91, 367
HpyCH4V TGCA 3 cut(s) 170, 335, 394
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 515, 604
HpyF3I CTNAG 2 cut(s) 306, 663
Hsp92II CATG 3 cut(s) 199, 244, 526
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 5 cut(s) 69, 102, 268, 487, 647
LmnI GCTCC 4 cut(s) 343, 595, 603, 640
LweI GCATC 5 cut(s) 46, 292, 496, 562, 580
MaeI CTAG 1 cut(s) 512
MalI GATC 5 cut(s) 71, 104, 270, 489, 649
MboI GATC 5 cut(s) 69, 102, 268, 487, 647
MboII GAAGA 4 cut(s) 16, 163, 382, 497
MflI RGATCY 2 cut(s) 487, 647
MhlI GDGCHC 3 cut(s) 39, 348, 600
MluCI AATT 3 cut(s) 388, 432, 671
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 9 cut(s) 51, 70, 74, 192, 283, 475, 565, 658, 702
MseI TTAA 2 cut(s) 183, 261
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 3 cut(s) 162, 581, 638
MspR9I CCNGG 2 cut(s) 162, 659
MvaI CCWGG 1 cut(s) 659
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 3 cut(s) 176, 515, 604
NciI CCSGG 1 cut(s) 162
NdeII GATC 5 cut(s) 69, 102, 268, 487, 647
NlaIII CATG 3 cut(s) 199, 244, 526
NspI RCATGY 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 189
PfeI GAWTC 2 cut(s) 373, 550
Psp124BI GAGCTC 2 cut(s) 348, 600
Psp6I CCWGG 1 cut(s) 657
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 657
PspPI GGNCC 3 cut(s) 159, 164, 409
PsuI RGATCY 2 cut(s) 487, 647
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 1 cut(s) 529
RsaNI GTAC 1 cut(s) 528
SacI GAGCTC 2 cut(s) 348, 600
SaqAI TTAA 2 cut(s) 183, 261
Sau3AI GATC 5 cut(s) 69, 102, 268, 487, 647
Sau96I GGNCC 3 cut(s) 159, 164, 409
ScrFI CCNGG 2 cut(s) 162, 659
SduI GDGCHC 3 cut(s) 39, 348, 600
SfaNI GCATC 5 cut(s) 46, 292, 496, 562, 580
SfcI CTRYAG 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 189
SinI GGWCC 2 cut(s) 164, 409
SlaI CTCGAG 1 cut(s) 189
SmlI CTYRAG 3 cut(s) 189, 206, 347
SmoI CTYRAG 3 cut(s) 189, 206, 347
Sse9I AATT 3 cut(s) 388, 432, 671
SsiI CCGC 3 cut(s) 593, 601, 708
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 2 cut(s) 348, 600
StyD4I CCNGG 2 cut(s) 160, 657
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 2 cut(s) 91, 367
TaqI TCGA 2 cut(s) 93, 190
TasI AATT 3 cut(s) 388, 432, 671
TfiI GAWTC 2 cut(s) 373, 550
Tru1I TTAA 2 cut(s) 183, 261
Tru9I TTAA 2 cut(s) 183, 261
TspDTI ATGAA 6 cut(s) 17, 111, 240, 374, 414, 444
TspGWI ACGGA 1 cut(s) 545
VpaK11BI GGWCC 2 cut(s) 164, 409
XagI CCTNNNNNAGG 1 cut(s) 64
XapI RAATTY 1 cut(s) 432
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 189
XspI CTAG 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.