Rorug03G0295000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003138
Physical Location & Seq
Reverse (-)
30367064 .. 30371161
4098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0295000.1

Sequence Viewer

Length: 3627 bp
ATGGAGACTCATCAGAAAGAAAACATAATCTCGTCCTGGCAACGTCAGCTTCAGTTTGATCAGTATTTTGTTGTCAATCCTGTAAATACTAGTAGTGGGGGTCTTGCCGTTTTTTGGAAATCTTCTGTTCAGGTGTCAATAGTCGAATATAACTCTAATGTTATTGACATGGATGTGTATTTTACTATTGAAGATTTCAGATGTAAAATATCCTGGATGTATGGCAATCCTGTTGAAAATAAAAAGAACTCCTTTTGGTGTTATATGTGTCATTATTTCTCAATTCAATCTATTCCTTGGATTTGTCTTGGAGATTTTAATGATGTCTTATACACTACTGAAAAGTGGGGTGGCTGTTCTCCCTCTCTGTGGAGAATGAATCTGTTTCGCAATTTTCTTAATCATTGTGCCCTGAGAGACCTTCATGCTCAGGGTCCTCTTCATACTTGGTACCGTTATCAGCATGGGCAAGTAAATATGAAGGAGCGCCTTGATCGCTGTTTAGCTAACTCAGAATGGTGCTCTTCTTGTCCTAATTCCCAGGTGATCAATCTTCCGATTATTGGTTCGGACCATCGTCCACTTCTTATGGATTCTAATCCAAAGGTGTTACCTTTCCGGAAATGCTTTCGGTTTGAGCACATATGGACTTCTGTAGATTCCTGTGAACAGGTTATTGCACAAGCTTGGCTGAATGCCTCCTCCTCAATAGCAATGCCTACTTGGATAGCCAATTTGAAGTCCTGCAAACAATCTTTGTTGGTCTGGAGCAAGCAAACTTTCCCGAATTCTCATAAACAGGTGGAGGAATATCTAACTCAACTAAATCATCTTCATAATACCTCTGGGTTGCACTCCGCTGACCAGATTCGAGTCCTTACCCTAAAAATTGAGGAGCTTTGGATTCTGGAGGAAAATTTTTGGCATCAGAGATCCAGGGTTGCTTGGTTGAAATTGGGAGATCAGAACTCCAAGTTTTTTCATCAGACTGCCACTCAACGAAATCAGAAAAACAAGATTCATCGACTTCGTAATGCTCAGGGTCAGTGGTTGGATTCCGAAGTAGACATTGCCAATACTTTTCTTCAGTTTTATCAGAAGTTGTTTACTTCCTCTGGACCAAGAGATTGGGCAGATATAATTCAGTTGATTGATCCGGTAATTACTACTGATATGAACAAAAGCCTTCTCGCTGCGGTCACTCTTGATGAGGTTCATTCTGCAATTTTTCAGCTAGGAGCATTGAAATCTCCTGGACCAGATGGGTTCCCAGGCCTTTTTTACCAAAAATATTGGGCTATAGTCAACTCATTGATTCATCAAGCTACCTCTTCTTTCTTTTTCAATGGAACTCTCTTGCCTGAGCTGAACCGCACTCATGTAGTCTTAATTCCGAAGGTACATCACCCAGAACATGCACACCAATTTCGTCCTATTGGTTTGTGCAACTTCTCTTACAAGGTTTTATCCAAAGTTCTAACAAATCGGCTTAAACCTTTAATGCCTGATATTATTTCAGAAAATCAATCTGCCTTTGTCTTAGGAAGGCAAATTCAAGACAATGTGATAGTGGCCCATGAGATGTTTCATCATCTCAAACTTCTTCGTCATGCTGGAATGGGTGCTTTTGGTCTAAAGTTAGATGTGGTGAAAGCATATGATTCGGTTGAATGGGATTTTCTCCAAGCTGTCCTTCTCAAACTAGTTGCTAATCCAGGAAAGTACTTGGGTGTTCCAATGATTTGGGGACGTTCTAAGTGCTCAGCTTTGGCTTACATTCGAGATTCTGTTGCTAGAAAATTGACAGGATGGAAACAACAATTCTTAACACAAGCTGGGAAGGAAGTTTTGATCAAGTCAGTAGCAACGGCCATTCCAGCTTATCCAATGAATTGTTTTCGTTTTCCAAAGACATTGTGCTCTCGGATTAACTCGGACATTGCTAACTTCTGGTGGGGAAAGAAAGGTGCAAATGGAATTCACTGGAAAAACTGGAAATTTCTTGGTCTTCCAAAATCTCAAGGTGGAATGGGTTTTCGAGACCTGGAGGATTTTAATCAGGCCTTATTAGCCAAGCAAGGGTGGAGGATTCTAACGAATCCTCAGTCTCTTTGGGTCAGAATGTTGAAAGCCAAGTACTTTCCTGATGTGCCTTTCTTGGCAGCTCGGATTGGATCTTCTCCTTCTTGGTTATGGTCAAGTTTATTACATGGGCAGCAGCTTCTCAAATCTAATCTTCTTTGGAGGGTGGGAAATGGTTTACATGTTACAATATGGAATACTAATTGGATTCCCGGTCTGCAGGGGTCTTCTTTAACCCCATCATCATCTTCGGATCCTTTACTACCTGTCTCTTCATTGATTAATTGGCCTACCTTATCCTGGGATTTAACCCGGCTTGTAGAACATACTTCTGAAGCTCAACGAGCAGCCATCCGGGTTATTCCTTTAATCAGCTCATCCATTCCGGATACTCTTGTCTGGCCTCTCAACAAATCCGGGATTTACACGGTTAAGTCAGGGTATTTTGCAGTTCACATGGCCTCTTCTCAACCAACTGTGCCTCATTCACATACTTCACATATTCCTGATTCATCAATTTGGAAGCGCCTTTGGTCCATCCAAACTCTTCCGAAAATTAGGAATTTCATGTGGAGAGGTTTGTCAAGTACTCTTGCAACAAAGGCTGCTTTATTTGCTCGTAAGTTATCTTTGGATCCAATCTGTTCTCTATGCCAATGTTTCCCTGAAACTTTGGAACACATTCTTTTTGCTTGTCCATGGGCGAGAAAGACTTGGATGTTTCATCCAATTGGTTACCGTCCCCAGCTTCAACAACTTACAACTTTTGATACTTGGTTCTATCAGCTCTCTTTCCCAGTTTCCACAGAAAAATCTCAGCAACAAGAGGTTATGTCTCATATTAGTTTCTTACTTTGGTACATATGGAAACATAGGTGTCATTGTGTATTTAATTATAGTACTCCAGATTCTAGATTGGTTGCATCTACAGCTTTTGATGCCTCTCTAGAATTCCTGTCCTCAAAGAAGAAGATGGTTACTCAATCAGTCAAATCCTCTCTACGATCCAGACCACCACCAAAACCAAAATGGATTCCTCCTCTGCCTCAACAACTGAAGATAAATAGTGATGCTTCTTGGACTTCTCAGTCTGTAGGTGTGGCTGCTATCGCTAGAGATAGTACAGGACACGTGGTGGGGGGTGTTCACTCATTCAGTTCAGCCCCAACTCCTCTCTATGCTGAAGCTCTAGCCCTTTTGGCGGCTACTAATCTTGCTCATAGCCTTATTGATCAGCATATTCAAGTAGATTCGTTTCTGTTTGAATCTGATTCCTTTCAACTAGTTGATCTCCTTAATAATTCAGGTCCATCTTCAGATTGGGCTGTGATGCCCCTGATAGATTATATTCGAGATCGTACAGTTGCTCTCCCTTCTTATAGCTGGACTTGGTCTAGCAGGTTGGCTAATCAAGCGGCAGATCACTTAGCTTGTTTGGCTCGTAGGAGAATGTGTCCGGATGATTGGTGGATTCATCCTCCTTCCTCCTTATCTTACTTCCTTCTCTTAGATGCTGTTTCTACTCCAGATTTCATTTCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1208

Amino Acids

137.96

Weight (kDa)

9.19

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 25 - 192 1.2e-06 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 473 - 568 2.9e-12 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 837 - 933 2.4e-14 zinc-binding in reverse transcriptase
RVT_3 PF13456 1049 - 1171 1.3e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3169
Acc36I ACCTGC 1 cut(s) 3471
Acc65I GGTACC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 1065
AccIII TCCGGA 3 cut(s) 618, 2467, 3538
AciI CCGC 5 cut(s) 858, 1196, 1372, 3284, 3497
AclWI GGATC 8 cut(s) 927, 1148, 2182, 2330, 2343, 2711, 2724, 3081
AcoI YGGCCR 1 cut(s) 1869
AcsI RAATTY 7 cut(s) 787, 916, 1551, 1977, 1997, 2644, 3032
AcuI CTGAAG 6 cut(s) 35, 1070, 2436, 3158, 3285, 3381
AcvI CACGTG 1 cut(s) 3214
AdeI CACNNNGTG 1 cut(s) 3217
AfaI GTAC 9 cut(s) 452, 1401, 1724, 2138, 2671, 2942, 2983, 3205, 3442
AfiI CCNNNNNNNGG 6 cut(s) 114, 369, 563, 2079, 2382, 3283
AflIII ACRYGT 2 cut(s) 2263, 3211
AhlI ACTAGT 3 cut(s) 89, 1702, 3364
AjnI CCWGG 9 cut(s) 35, 212, 540, 935, 1252, 1270, 1714, 2043, 2381
AjuI GAANNNNNNNTTGG 2 cut(s) 1677, 1709
Alw21I GWGCWC 4 cut(s) 524, 642, 1763, 1922
Alw26I GTCTC 5 cut(s) 411, 2034, 2112, 2356, 2922
AlwI GGATC 8 cut(s) 927, 1148, 2182, 2330, 2343, 2711, 2724, 3081
Aor13HI TCCGGA 3 cut(s) 618, 2467, 3538
AoxI GGCC 7 cut(s) 1273, 1572, 1869, 2061, 2369, 2483, 2541
ApeKI GCWGC 7 cut(s) 1193, 2162, 2215, 2218, 2429, 2687, 3185
ApoI RAATTY 7 cut(s) 787, 916, 1551, 1977, 1997, 2644, 3032
ArsI GACNNNNNNTTYG 2 cut(s) 2090, 2122
AseI ATTAAT 1 cut(s) 2364
Asp700I GAANNNNTTC 1 cut(s) 2763
Asp718I GGTACC 1 cut(s) 450
AspLEI GCGC 2 cut(s) 489, 2610
AspS9I GGNCC 7 cut(s) 434, 571, 1118, 1256, 1573, 2616, 3389
AsuC2I CCSGG 4 cut(s) 2295, 2395, 2438, 2500
AsuHPI GGTGA 3 cut(s) 556, 1397, 1660
AvaII GGWCC 6 cut(s) 434, 571, 1118, 1256, 2616, 3389
BaeGI GKGCMC 1 cut(s) 412
BaeI ACNNNNGTAYC 2 cut(s) 2463, 2496
BamHI GGATCC 2 cut(s) 2335, 2716
BanI GGYRCC 1 cut(s) 450
BbrPI CACGTG 1 cut(s) 3214
BbsI GAAGAC 2 cut(s) 2000, 2301
Bbv12I GWGCWC 4 cut(s) 524, 642, 1763, 1922
BbvI GCAGC 7 cut(s) 1180, 2174, 2227, 2230, 2441, 2674, 3172
BccI CCATC 8 cut(s) 582, 1256, 1803, 2329, 2441, 2627, 3049, 3400
BceAI ACGGC 2 cut(s) 92, 1884
BcgI CGANNNNNNTGC 2 cut(s) 1644, 1678
BciT130I CCWGG 9 cut(s) 37, 214, 542, 937, 1254, 1272, 1716, 2045, 2383
BciVI GTATCC 1 cut(s) 2464
BclI TGATCA 4 cut(s) 58, 546, 1851, 3313
BcnI CCSGG 4 cut(s) 2295, 2395, 2438, 2500
BcoDI GTCTC 5 cut(s) 411, 2034, 2112, 2356, 2922
BcuI ACTAGT 3 cut(s) 89, 1702, 3364
BfmI CTRYAG 5 cut(s) 654, 1299, 2300, 3009, 3174
BfoI RGCGCY 2 cut(s) 490, 2611
BfuAI ACCTGC 1 cut(s) 3471
BfuI GTATCC 1 cut(s) 2464
BglI GCCNNNNNGGC 1 cut(s) 3281
BisI GCNGC 9 cut(s) 1194, 2163, 2216, 2219, 2430, 2688, 3186, 3285, 3498
BlpI GCTNAGC 1 cut(s) 1762
BlsI GCNGC 9 cut(s) 1195, 2164, 2217, 2220, 2431, 2689, 3187, 3286, 3499
BmcAI AGTACT 4 cut(s) 1724, 2138, 2671, 2983
Bme18I GGWCC 6 cut(s) 434, 571, 1118, 1256, 2616, 3389
BmgT120I GGNCC 7 cut(s) 434, 571, 1118, 1256, 1573, 2616, 3389
BmiI GGNNCC 5 cut(s) 435, 452, 1268, 2337, 2718
BmrI ACTGGG 1 cut(s) 2873
BmsI GCATC 6 cut(s) 934, 3010, 3014, 3142, 3402, 3583
BmuI ACTGGG 1 cut(s) 2873
BoxI GACNNNNGTC 1 cut(s) 576
BpiI GAAGAC 2 cut(s) 2000, 2301
BpmI CTGGAG 5 cut(s) 787, 929, 2066, 2970, 3591
Bpu10I CCTNAGC 3 cut(s) 429, 1038, 1362
Bpu1102I GCTNAGC 1 cut(s) 1762
BpuEI CTTGAG 1 cut(s) 2004
BpuMI CCSGG 4 cut(s) 2295, 2395, 2438, 2500
BsaAI YACGTR 1 cut(s) 3214
BsaBI GATNNNNATC 2 cut(s) 597, 2055
BsaI GGTCTC 2 cut(s) 411, 2034
BsaJI CCNNGG 6 cut(s) 296, 540, 936, 1270, 2382, 2780
BsaWI WCCGGW 4 cut(s) 618, 1156, 2467, 3538
BsaXI ACNNNNNCTCC 2 cut(s) 3520, 3550
Bsc4I CCNNNNNNNGG 6 cut(s) 114, 369, 563, 2079, 2382, 3283
Bse1I ACTGG 3 cut(s) 1988, 1997, 2879
Bse3DI GCAATG 3 cut(s) 720, 1068, 1938
Bse8I GATNNNNATC 2 cut(s) 597, 2055
BseAI TCCGGA 3 cut(s) 618, 2467, 3538
BseBI CCWGG 9 cut(s) 37, 214, 542, 937, 1254, 1272, 1716, 2045, 2383
BseDI CCNNGG 6 cut(s) 296, 540, 936, 1270, 2382, 2780
BseJI GATNNNNATC 2 cut(s) 597, 2055
BseLI CCNNNNNNNGG 6 cut(s) 114, 369, 563, 2079, 2382, 3283
BseMI GCAATG 3 cut(s) 720, 1068, 1938
BseMII CTCAG 9 cut(s) 404, 443, 525, 1052, 1353, 1776, 2117, 2912, 3182
BseNI ACTGG 3 cut(s) 1988, 1997, 2879
BseRI GAGGAG 5 cut(s) 691, 694, 908, 3111, 3243
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 7 cut(s) 1180, 2174, 2227, 2230, 2441, 2674, 3172
BseYI CCCAGC 2 cut(s) 1835, 2826
BshFI GGCC 7 cut(s) 1275, 1574, 1871, 2063, 2371, 2485, 2543
BshNI GGYRCC 1 cut(s) 450
BsiHKAI GWGCWC 4 cut(s) 524, 642, 1763, 1922
BsiSI CCGG 8 cut(s) 619, 1157, 2295, 2395, 2437, 2468, 2499, 3539
BslFI GGGAC 2 cut(s) 1761, 2808
BslI CCNNNNNNNGG 6 cut(s) 114, 369, 563, 2079, 2382, 3283
BsmAI GTCTC 5 cut(s) 411, 2034, 2112, 2356, 2922
BsmFI GGGAC 2 cut(s) 1761, 2808
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 7 cut(s) 1275, 1574, 1871, 2063, 2371, 2485, 2543
Bso31I GGTCTC 2 cut(s) 411, 2034
Bsp1286I GDGCHC 5 cut(s) 412, 524, 642, 1763, 1922
Bsp13I TCCGGA 3 cut(s) 618, 2467, 3538
Bsp1720I GCTNAGC 1 cut(s) 1762
Bsp19I CCATGG 1 cut(s) 2780
BspACI CCGC 5 cut(s) 858, 1196, 1372, 3284, 3497
BspANI GGCC 7 cut(s) 1275, 1574, 1871, 2063, 2371, 2485, 2543
BspCNI CTCAG 9 cut(s) 405, 442, 524, 1051, 1354, 1775, 2116, 2911, 3181
BspEI TCCGGA 3 cut(s) 618, 2467, 3538
BspLI GGNNCC 5 cut(s) 435, 452, 1268, 2337, 2718
BspMAI CTGCAG 1 cut(s) 2304
BspMI ACCTGC 1 cut(s) 3471
BspPI GGATC 8 cut(s) 927, 1148, 2182, 2330, 2343, 2711, 2724, 3081
BspQI GCTCTTC 1 cut(s) 529
BspT107I GGYRCC 1 cut(s) 450
BspTNI GGTCTC 2 cut(s) 411, 2034
BsrDI GCAATG 3 cut(s) 720, 1068, 1938
BsrI ACTGG 3 cut(s) 1988, 1997, 2879
BssECI CCNNGG 6 cut(s) 296, 540, 936, 1270, 2382, 2780
BssT1I CCWWGG 2 cut(s) 296, 2780
Bst2UI CCWGG 9 cut(s) 37, 214, 542, 937, 1254, 1272, 1716, 2045, 2383
Bst4CI ACNGT 5 cut(s) 455, 2512, 2560, 2822, 3445
Bst6I CTCTTC 6 cut(s) 444, 529, 1336, 2359, 2551, 2634
BstBAI YACGTR 1 cut(s) 3214
BstC8I GCNNGC 1 cut(s) 773
BstDSI CCRYGG 1 cut(s) 2780
BstEII GGTNACC 1 cut(s) 2816
BstH2I RGCGCY 2 cut(s) 490, 2611
BstHHI GCGC 2 cut(s) 489, 2610
BstMAI GTCTC 5 cut(s) 411, 2034, 2112, 2356, 2922
BstNI CCWGG 9 cut(s) 37, 214, 542, 937, 1254, 1272, 1716, 2045, 2383
BstNSI RCATGY 2 cut(s) 1418, 2267
BstPAI GACNNNNGTC 1 cut(s) 576
BstPI GGTNACC 1 cut(s) 2816
BstSFI CTRYAG 5 cut(s) 654, 1299, 2300, 3009, 3174
BstSLI GKGCMC 1 cut(s) 412
BstV1I GCAGC 7 cut(s) 1180, 2174, 2227, 2230, 2441, 2674, 3172
BstV2I GAAGAC 2 cut(s) 2000, 2301
BstX2I RGATCY 4 cut(s) 932, 2174, 2335, 2716
BstXI CCANNNNNNTGG 2 cut(s) 1128, 1743
BstYI RGATCY 4 cut(s) 932, 2174, 2335, 2716
BsuI GTATCC 1 cut(s) 2464
BsuRI GGCC 7 cut(s) 1275, 1574, 1871, 2063, 2371, 2485, 2543
BtgI CCRYGG 1 cut(s) 2780
BtsIMutI CAGTG 2 cut(s) 1052, 1981
BveI ACCTGC 1 cut(s) 3471
Cac8I GCNNGC 1 cut(s) 773
CfoI GCGC 2 cut(s) 489, 2610
Cfr13I GGNCC 7 cut(s) 434, 571, 1118, 1256, 1573, 2616, 3389
Csp6I GTAC 9 cut(s) 451, 1400, 1723, 2137, 2670, 2941, 2982, 3204, 3441
CviQI GTAC 9 cut(s) 451, 1400, 1723, 2137, 2670, 2941, 2982, 3204, 3441
DraIII CACNNNGTG 1 cut(s) 3217
DrdI GACNNNNNNGTC 1 cut(s) 3169
DseDI GACNNNNNNGTC 1 cut(s) 3169
EaeI YGGCCR 1 cut(s) 1869
Eam1104I CTCTTC 6 cut(s) 444, 529, 1336, 2359, 2551, 2634
EarI CTCTTC 6 cut(s) 444, 529, 1336, 2359, 2551, 2634
Eco130I CCWWGG 2 cut(s) 296, 2780
Eco147I AGGCCT 2 cut(s) 1275, 2063
Eco31I GGTCTC 2 cut(s) 411, 2034
Eco47I GGWCC 6 cut(s) 434, 571, 1118, 1256, 2616, 3389
Eco57I CTGAAG 6 cut(s) 35, 1070, 2436, 3158, 3285, 3381
Eco72I CACGTG 1 cut(s) 3214
Eco91I GGTNACC 1 cut(s) 2816
EcoO109I RGGNCCY 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 2816
EcoRI GAATTC 3 cut(s) 787, 1977, 3032
EcoRII CCWGG 9 cut(s) 35, 212, 540, 935, 1252, 1270, 1714, 2043, 2381
EcoT14I CCWWGG 2 cut(s) 296, 2780
ErhI CCWWGG 2 cut(s) 296, 2780
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 1677, 1709
FaqI GGGAC 2 cut(s) 1761, 2808
FauNDI CATATG 3 cut(s) 644, 1657, 2945
FbaI TGATCA 4 cut(s) 58, 546, 1851, 3313
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 9 cut(s) 1194, 2163, 2216, 2219, 2430, 2688, 3186, 3285, 3498
Fsp4HI GCNGC 9 cut(s) 1194, 2163, 2216, 2219, 2430, 2688, 3186, 3285, 3498
GlaI GCGC 2 cut(s) 488, 2609
GluI GCNGC 9 cut(s) 1194, 2163, 2216, 2219, 2430, 2688, 3186, 3285, 3498
GsaI CCCAGC 2 cut(s) 1839, 2830
GsuI CTGGAG 5 cut(s) 787, 929, 2066, 2970, 3591
HaeII RGCGCY 2 cut(s) 490, 2611
HaeIII GGCC 7 cut(s) 1275, 1574, 1871, 2063, 2371, 2485, 2543
HapII CCGG 8 cut(s) 619, 1157, 2295, 2395, 2437, 2468, 2499, 3539
HhaI GCGC 2 cut(s) 489, 2610
Hin6I GCGC 2 cut(s) 487, 2608
HinP1I GCGC 2 cut(s) 487, 2608
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HindIII AAGCTT 1 cut(s) 684
HpaII CCGG 8 cut(s) 619, 1157, 2295, 2395, 2437, 2468, 2499, 3539
HphI GGTGA 3 cut(s) 556, 1397, 1660
Hpy166II GTNNAC 8 cut(s) 581, 668, 1066, 1107, 1306, 2261, 2536, 3229
Hpy8I GTNNAC 8 cut(s) 581, 668, 1066, 1107, 1306, 2261, 2536, 3229
HpyCH4III ACNGT 5 cut(s) 455, 2512, 2560, 2822, 3445
HpyCH4IV ACGT 3 cut(s) 43, 1750, 3213
HpySE526I ACGT 3 cut(s) 43, 1750, 3213
HspAI GCGC 2 cut(s) 487, 2608
Kpn2I TCCGGA 3 cut(s) 618, 2467, 3538
KpnI GGTACC 1 cut(s) 454
Ksp22I TGATCA 4 cut(s) 58, 546, 1851, 3313
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 4 cut(s) 484, 768, 895, 1238
Lsp1109I GCAGC 7 cut(s) 1180, 2174, 2227, 2230, 2441, 2674, 3172
LweI GCATC 6 cut(s) 934, 3010, 3014, 3142, 3402, 3583
MaeII ACGT 3 cut(s) 43, 1750, 3213
MaeIII GTNAC 5 cut(s) 609, 1198, 2266, 2816, 3058
MfeI CAATTG 1 cut(s) 2811
MflI RGATCY 4 cut(s) 932, 2174, 2335, 2716
MhlI GDGCHC 5 cut(s) 412, 524, 642, 1763, 1922
MlyI GAGTC 1 cut(s) 882
MmeI TCCRAC 1 cut(s) 1032
MroI TCCGGA 3 cut(s) 618, 2467, 3538
MroXI GAANNNNTTC 1 cut(s) 2763
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 8 cut(s) 619, 1157, 2295, 2395, 2437, 2468, 2499, 3539
MunI CAATTG 1 cut(s) 2811
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 9 cut(s) 37, 214, 542, 937, 1254, 1272, 1716, 2045, 2383
NciI CCSGG 4 cut(s) 2295, 2395, 2438, 2500
NcoI CCATGG 1 cut(s) 2780
NdeI CATATG 3 cut(s) 644, 1657, 2945
NlaIV GGNNCC 5 cut(s) 435, 452, 1268, 2337, 2718
NmuCI GTSAC 1 cut(s) 1198
NspI RCATGY 2 cut(s) 1418, 2267
PceI AGGCCT 2 cut(s) 1275, 2063
PciI ACATGT 1 cut(s) 2263
PciSI GCTCTTC 1 cut(s) 529
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 2763
PflFI GACNNNGTC 1 cut(s) 3472
PfoI TCCNGGA 4 cut(s) 212, 1252, 1714, 2498
PkrI GCNGC 9 cut(s) 1195, 2164, 2217, 2220, 2431, 2689, 3187, 3286, 3499
PleI GAGTC 1 cut(s) 881
PmaCI CACGTG 1 cut(s) 3214
PmlI CACGTG 1 cut(s) 3214
PpsI GAGTC 1 cut(s) 881
Ppu21I YACGTR 1 cut(s) 3214
PpuMI RGGWCCY 1 cut(s) 434
PscI ACATGT 1 cut(s) 2263
PshAI GACNNNNGTC 1 cut(s) 576
PshBI ATTAAT 1 cut(s) 2364
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 9 cut(s) 35, 212, 540, 935, 1252, 1270, 1714, 2043, 2381
PspCI CACGTG 1 cut(s) 3214
PspEI GGTNACC 1 cut(s) 2816
PspFI CCCAGC 2 cut(s) 1835, 2826
PspGI CCWGG 9 cut(s) 35, 212, 540, 935, 1252, 1270, 1714, 2043, 2381
PspN4I GGNNCC 5 cut(s) 435, 452, 1268, 2337, 2718
PspPI GGNCC 7 cut(s) 434, 571, 1118, 1256, 1573, 2616, 3389
PspPPI RGGWCCY 1 cut(s) 434
PstI CTGCAG 1 cut(s) 2304
PsuI RGATCY 4 cut(s) 932, 2174, 2335, 2716
PsyI GACNNNGTC 1 cut(s) 3472
RsaI GTAC 9 cut(s) 452, 1401, 1724, 2138, 2671, 2942, 2983, 3205, 3442
RsaNI GTAC 9 cut(s) 451, 1400, 1723, 2137, 2670, 2941, 2982, 3204, 3441
RseI CAYNNNNRTG 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 529
SatI GCNGC 9 cut(s) 1194, 2163, 2216, 2219, 2430, 2688, 3186, 3285, 3498
Sau96I GGNCC 7 cut(s) 434, 571, 1118, 1256, 1573, 2616, 3389
ScaI AGTACT 4 cut(s) 1724, 2138, 2671, 2983
SchI GAGTC 1 cut(s) 882
SduI GDGCHC 5 cut(s) 412, 524, 642, 1763, 1922
SfaNI GCATC 6 cut(s) 934, 3010, 3014, 3142, 3402, 3583
SfcI CTRYAG 5 cut(s) 654, 1299, 2300, 3009, 3174
SinI GGWCC 6 cut(s) 434, 571, 1118, 1256, 2616, 3389
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 2019
SmoI CTYRAG 1 cut(s) 2019
SpeI ACTAGT 3 cut(s) 89, 1702, 3364
SseBI AGGCCT 2 cut(s) 1275, 2063
SsiI CCGC 5 cut(s) 858, 1196, 1372, 3284, 3497
SspI AATATT 1 cut(s) 1292
StuI AGGCCT 2 cut(s) 1275, 2063
StyI CCWWGG 2 cut(s) 296, 2780
TaaI ACNGT 5 cut(s) 455, 2512, 2560, 2822, 3445
TaiI ACGT 3 cut(s) 46, 1753, 3216
TaqI TCGA 6 cut(s) 144, 871, 1024, 1780, 2038, 3433
TatI WGTACW 5 cut(s) 1722, 2136, 2669, 2981, 3203
TauI GCSGC 2 cut(s) 3287, 3500
TscAI CASTG 2 cut(s) 1052, 1988
TseFI GTSAC 1 cut(s) 1198
TseI GCWGC 7 cut(s) 1193, 2162, 2215, 2218, 2429, 2687, 3185
Tsp45I GTSAC 1 cut(s) 1198
TspRI CASTG 2 cut(s) 1052, 1988
Tth111I GACNNNGTC 1 cut(s) 3472
VpaK11BI GGWCC 6 cut(s) 434, 571, 1118, 1256, 2616, 3389
VspI ATTAAT 1 cut(s) 2364
XapI RAATTY 7 cut(s) 787, 916, 1551, 1977, 1997, 2644, 3032
XbaI TCTAGA 2 cut(s) 2993, 3028
XceI RCATGY 2 cut(s) 1418, 2267
XcmI CCANNNNNNNNNTGG 2 cut(s) 3108, 3399
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 1 cut(s) 2763
ZrmI AGTACT 4 cut(s) 1724, 2138, 2671, 2983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.