Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003333

Physical Location & Seq

Genomic Coordinates Forward (+)

28436628 .. 28441709

Gene Structure 5082 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1221800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGGCCTCCTCTATCATTTGGTTCTCTGTTTTCCCCTGCTCCTTTTTGTTTCAATACTTGTTATTCTTTTCGCCAAATTTTAAGGGCGTTTTAGGCCAATCGTCGCCGGTTTTCGCTTGCGATGTCGCTAGCAACGCAACCCTATCGAGCTTCGCCTTCTGCGATACGTCCTTGGGGGTCGATGCGAGAGTGGCGGACTTGGTGAAGCGGCTGACATTGCCGGAGAAAATCGGGTTTTTGGTGAACAGTGCAGGGAGTGTGAGCAGGCTTGGAATTCCCAAATACGAGTGGTGGTCAGAAGCATTACACGGCGTCTCATACGTCGGCCCCGGCACCAAGTTTTCGAGCCTCGTTCCCGGAGCTACCAGCTTTCCTCAGGTCATCCTCACTGCCGCTTCTTTCAATACTTCTCTCTTTGAAGCCATTGGCAGGGTGGTTTCGACGGAGGCCAGAGCAATGTACAATGTGGGATTGGCAGGACTGACATTTTGGTCACCAAATATTAACATATTTCGAGACCCCAGATGGGGAAGAGGCCAGGAGACTCCGGGTGAAGATCCGTTGCTCTCGAGTAAATACGGATCCGGATATGTTCGAGGCCTTCAACAAACCGACGATGGTGACAAGAATAAGCTTAAGGTTGCTGCATGCTGCAAACATTATACAGCCTACGATGTAGACAATTGGAAAGGGGTTGACAGATACCATTTTAATGCAGTGGTAACGAAACAAGATCTGGACGACACATTTCAACCGCCATTCAAAAGTTGTGTTATCGATGGCAATGTTGCAAGTGTCATGTGTTCTTACAACCAGGTTAACGGGATACCAACCTGTGCAGACCCCGACCTTCTTGCTGGAGTTATCCGAGGCGAATGGAAACTAAATGGATACATTGTTACGGATTGTGATTCAATAGATGTGTACTACAAGCAGCAACACTACACCAAAACACCAGAGGAAGCTGCAGCTAAGGCTATATTGGCAGGGTTGGATCTCAACTGCGGATCTTTCCTCGGACAATACACGCCAGGTGCAGTCCAAGCAGGACTTGTGACGGAGGCCGACATTGACAGGGCCATCTCCAACAATTTTGCTACTTTGATGCGTCTAGGCTTCTTTGATGGTGATCCCAGCGACAAACCCTATGGAAAGCTTGGTCCAAAAGATGTGTGTACCTCAGAGAACCAGGAGCTAGCCCGTGAAACCGCCAGGCAAGGGATAGTACTGCTAAAAAACAGTCCGGGATCACTGCCTCTGTCTCCCACGGCCATCAAATCCTTGGCGGTCATTGGTCCCAATGCCAATGTCCAAAAGACAATGATCGGAAACTACGAAGGTACGCCGTGCAAGTATACAACTATTCTGCAAGGCCTAAGTGCCTCAGTTGCAACGAGTTATGTTGCCGGCTGCGCCAATGTGGCTTGTGGTACTGCACAGGTAGATGATGCTACCAAGATAGCAGCCTCAGCAGATGCAACTATACTTGTCGTGGGTGCAGATCAATCGATTGAGAGAGAAAGTCTTGACAGGATTGACCTGCTTCTTCCGGGACAGCAAACACTCCTCGTAACAGAAGTTGCAAAGGCAGCAAAAGGCCCAGTAATCCTTGTCATAATGTCTGGAGGAGGGTTCGACATTACATTTGCCAAAAACGATACTAAAATTACAAGCATACTTTGGGTTGGTTACCCCGGCGAGGCTGGTGGAGCTGCGGTAGCTGATGTAGTTTTTGGTCAATATAATCCAAGCGGGAGACTACCTATGACATGGTATCCACAATCTTATGTAGACAAGGTTCCAATGACAAACATGAACATGAGACCGGATCCTTCGAACGGTTACCCTGGCCGGACTTACAGATTTTTCACTGGTGAAACCGTTTACTCCTTTGGAGATGGACTAGGCTACTCCACCTTCAATCACAAACTAGTTCGAGCGCCTAAGCTGGTATCGATTCCCTTAGAAGAGGGTCATGTATGTCACTCATCAAGCTGCAAGTCACTTGACGTTGTGGAAGAAAGATGCGAAAACCTAGCTTTTGATATACATTTGGGGGTGAAGAACATGGGAAGTATGAGTGGAAGTCATACGGTGATGCTGTTTTCGAGTCCTCCGGCAGTGCACAAGTCGCCGCAGAAGCACTTGCTGGGTTTTGAGAAGGTGTTCGTGAGTGCTCAGAGAGTAGCATTGGTTAAGTTCAACGTGGATGTTTGCAAGCACCTGAGCGTGGTCGATGAGCTTGGAAACAGAAAGGTAGCTTTGGGAGAGCATGTGCTTCATGTGGGAAGCTTGAAACACTCTTTCAGCGTTAGGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0031221 GO:0031222 GO:0043170 GO:0043207 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044464 GO:0045087 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:0098542 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

774

Amino Acids

83.33

Weight (kDa)

6.33

Isoelectric Point (pI)

29.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	120 - 361	2.6e-33	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	408 - 637	1e-50	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	697 - 764	4e-09	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09730 AT5G64570 AT5G64570 AT5G64570
fragaria_vesca	FvH4_5g30690 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710
malus_domestica	MD08G1221800.v1.1 MD15G1414100.v1.1
prunus_persica	Prupe.1G559400_v2.0.a1 Prupe.1G559400_v2.0.a1
pyrus_communis	pycom08g19240 pycom15g36840
rosa_chinensis	RchiOBHm_Chr7g0225551 RchiOBHm_Chr7g0225581
rosa_laevigata	RLG00000001804 RLG00000001806
rosa_multiflora	Rmu_co8377913.1_g000001 Rmu_sc0003876.1_g000021 Rmu_sc0006321.1_g000006
rosa_roxburghii	Rroxscaffold_3G00233670 Rroxscaffold_3G00233690
rosa_rugosa	Rorug07G0231600 Rorug07G0231800
rosa_samantha	Rh7AG372300 Rh7AG372500 Rh7BG365400 Rh7BG365500 Rh7CG390600 Rh7CG390800 Rh7DG376200 Rh7DG376300
rosa_wichuraiana	Rw7G031750 Rw7G031760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	490
Acc36I	ACCTGC	1 cut(s)	1548
AccB1I	GGYRCC	1 cut(s)	332
AccI	GTMKAC	3 cut(s)	678, 1355, 1791
AccIII	TCCGGA	1 cut(s)	584
AclWI	GGATC	9 cut(s)	551, 576, 589, 1002, 1015, 1124, 1255, 1823, 1836
AcoI	YGGCCR	2 cut(s)	1269, 1849
AcsI	RAATTY	2 cut(s)	76, 273
AcyI	GRCGYC	1 cut(s)	312
AfaI	GTAC	6 cut(s)	461, 926, 1177, 1227, 1342, 1432
AfiI	CCNNNNNNNGG	7 cut(s)	429, 526, 527, 1218, 1699, 1838, 2230
AflII	CTTAAG	1 cut(s)	635
AhlI	ACTAGT	1 cut(s)	1930
AjnI	CCWGG	6 cut(s)	537, 813, 1030, 1188, 1211, 1846
Alw21I	GWGCWC	2 cut(s)	2127, 2179
Alw26I	GTCTC	6 cut(s)	319, 510, 536, 1266, 1750, 1816
Alw44I	GTGCAC	1 cut(s)	2123
AlwI	GGATC	9 cut(s)	551, 576, 589, 1002, 1015, 1124, 1255, 1823, 1836
Ama87I	CYCGRG	1 cut(s)	568
Aor13HI	TCCGGA	1 cut(s)	584
ApaLI	GTGCAC	1 cut(s)	2123
ApoI	RAATTY	2 cut(s)	76, 273
ArsI	GACNNNNNNTTYG	4 cut(s)	471, 503, 1628, 1660
Asp700I	GAANNNNTTC	1 cut(s)	2165
AspLEI	GCGC	2 cut(s)	1415, 1942
AspS9I	GGNCC	5 cut(s)	326, 1077, 1160, 1295, 1598
AsuC2I	CCSGG	6 cut(s)	330, 357, 549, 1245, 1551, 1695
AsuHPI	GGTGA	9 cut(s)	214, 253, 486, 563, 632, 1139, 1886, 2071, 2107
AsuII	TTCGAA	1 cut(s)	1835
AsuNHI	GCTAGC	2 cut(s)	128, 1195
AvaI	CYCGRG	1 cut(s)	568
AvaII	GGWCC	2 cut(s)	1160, 1295
AxyI	CCTNAGG	1 cut(s)	375
BaeGI	GKGCMC	1 cut(s)	2127
BamHI	GGATCC	2 cut(s)	581, 1828
BanI	GGYRCC	1 cut(s)	332
Bbv12I	GWGCWC	2 cut(s)	2127, 2179
BbvCI	CCTCAGC	1 cut(s)	1468
BccI	CCATC	7 cut(s)	519, 611, 773, 1088, 1118, 1280, 1892
BceAI	ACGGC	3 cut(s)	325, 1284, 1330
BcgI	CGANNNNNNTGC	6 cut(s)	126, 160, 836, 870, 1488, 1522
BciT130I	CCWGG	6 cut(s)	539, 815, 1032, 1190, 1213, 1848
BciVI	GTATCC	3 cut(s)	819, 884, 1785
BcnI	CCSGG	6 cut(s)	330, 357, 549, 1245, 1551, 1695
BcoDI	GTCTC	6 cut(s)	319, 510, 536, 1266, 1750, 1816
BcuI	ACTAGT	1 cut(s)	1930
BfaI	CTAG	6 cut(s)	129, 1112, 1196, 1904, 1931, 2036
BfmI	CTRYAG	1 cut(s)	966
BfoI	RGCGCY	1 cut(s)	1943
BfrI	CTTAAG	1 cut(s)	635
BfuAI	ACCTGC	1 cut(s)	1548
BfuI	GTATCC	3 cut(s)	819, 884, 1785
BglI	GCCNNNNNGGC	1 cut(s)	1421
BglII	AGATCT	1 cut(s)	733
BmcAI	AGTACT	1 cut(s)	1227
Bme18I	GGWCC	2 cut(s)	1160, 1295
BmeT110I	CYCGRG	1 cut(s)	568
BmgT120I	GGNCC	5 cut(s)	326, 1077, 1160, 1295, 1598
BmiI	GGNNCC	6 cut(s)	328, 334, 583, 1297, 1800, 1830
BmrI	ACTGGG	1 cut(s)	1595
BmsI	GCATC	6 cut(s)	172, 1095, 1438, 1465, 2015, 2088
BmtI	GCTAGC	2 cut(s)	132, 1199
BmuI	ACTGGG	1 cut(s)	1595
BpmI	CTGGAG	2 cut(s)	879, 1644
Bpu10I	CCTNAGC	4 cut(s)	972, 1468, 1944, 2225
Bpu14I	TTCGAA	1 cut(s)	1835
BpuMI	CCSGG	6 cut(s)	330, 357, 549, 1245, 1551, 1695
Bsa29I	ATCGAT	3 cut(s)	777, 1508, 1955
BsaBI	GATNNNNATC	1 cut(s)	1128
BsaHI	GRCGYC	1 cut(s)	312
BsaI	GGTCTC	2 cut(s)	510, 1816
BsaJI	CCNNGG	8 cut(s)	171, 328, 868, 1015, 1266, 1281, 1693, 1846
BsaWI	WCCGGW	2 cut(s)	584, 1825
BsaXI	ACNNNNNCTCC	4 cut(s)	1701, 1731, 2259, 2289
Bsc4I	CCNNNNNNNGG	7 cut(s)	429, 526, 527, 1218, 1699, 1838, 2230
Bse118I	RCCGGY	2 cut(s)	106, 1406
Bse1I	ACTGG	2 cut(s)	1601, 1876
Bse21I	CCTNAGG	1 cut(s)	375
Bse3DI	GCAATG	3 cut(s)	215, 462, 790
Bse8I	GATNNNNATC	1 cut(s)	1128
BseAI	TCCGGA	1 cut(s)	584
BseBI	CCWGG	6 cut(s)	539, 815, 1032, 1190, 1213, 1848
BseCI	ATCGAT	3 cut(s)	777, 1508, 1955
BseDI	CCNNGG	8 cut(s)	171, 328, 868, 1015, 1266, 1281, 1693, 1846
BseGI	GGATG	2 cut(s)	381, 2215
BseJI	GATNNNNATC	1 cut(s)	1128
BseLI	CCNNNNNNNGG	7 cut(s)	429, 526, 527, 1218, 1699, 1838, 2230
BseMI	GCAATG	3 cut(s)	215, 462, 790
BseMII	CTCAG	6 cut(s)	389, 1194, 1398, 1482, 2192, 2216
BseNI	ACTGG	2 cut(s)	1601, 1876
BseRI	GAGGAG	2 cut(s)	1556, 1641
BseSI	GKGCMC	1 cut(s)	2127
BseYI	CCCAGC	2 cut(s)	1133, 2149
BsgI	GTGCAG	5 cut(s)	270, 858, 1056, 1419, 1518
BshNI	GGYRCC	1 cut(s)	332
BshVI	ATCGAT	3 cut(s)	777, 1508, 1955
BsiHKAI	GWGCWC	2 cut(s)	2127, 2179
BsiHKCI	CYCGRG	1 cut(s)	568
BslFI	GGGAC	2 cut(s)	1281, 1566
BslI	CCNNNNNNNGG	7 cut(s)	429, 526, 527, 1218, 1699, 1838, 2230
BsmAI	GTCTC	6 cut(s)	319, 510, 536, 1266, 1750, 1816
BsmBI	CGTCTC	1 cut(s)	319
BsmFI	GGGAC	2 cut(s)	1281, 1566
Bso31I	GGTCTC	2 cut(s)	510, 1816
BsoBI	CYCGRG	1 cut(s)	568
Bsp119I	TTCGAA	1 cut(s)	1835
Bsp1286I	GDGCHC	2 cut(s)	2127, 2179
Bsp13I	TCCGGA	1 cut(s)	584
Bsp1407I	TGTACA	1 cut(s)	459
BspCNI	CTCAG	6 cut(s)	388, 1193, 1397, 1481, 2191, 2217
BspDI	ATCGAT	3 cut(s)	777, 1508, 1955
BspEI	TCCGGA	1 cut(s)	584
BspLI	GGNNCC	6 cut(s)	328, 334, 583, 1297, 1800, 1830
BspMAI	CTGCAG	1 cut(s)	970
BspMI	ACCTGC	1 cut(s)	1548
BspOI	GCTAGC	2 cut(s)	132, 1199
BspPI	GGATC	9 cut(s)	551, 576, 589, 1002, 1015, 1124, 1255, 1823, 1836
BspT104I	TTCGAA	1 cut(s)	1835
BspT107I	GGYRCC	1 cut(s)	332
BspTI	CTTAAG	1 cut(s)	635
BspTNI	GGTCTC	2 cut(s)	510, 1816
BsrDI	GCAATG	3 cut(s)	215, 462, 790
BsrFI	RCCGGY	2 cut(s)	106, 1406
BsrGI	TGTACA	1 cut(s)	459
BsrI	ACTGG	2 cut(s)	1601, 1876
BssAI	RCCGGY	2 cut(s)	106, 1406
BssECI	CCNNGG	8 cut(s)	171, 328, 868, 1015, 1266, 1281, 1693, 1846
BssNAI	GTATAC	1 cut(s)	1356
BssNI	GRCGYC	1 cut(s)	312
BssT1I	CCWWGG	2 cut(s)	171, 1281
Bst1107I	GTATAC	1 cut(s)	1356
Bst2UI	CCWGG	6 cut(s)	539, 815, 1032, 1190, 1213, 1848
Bst4CI	ACNGT	5 cut(s)	248, 1241, 1841, 1882, 2095
Bst6I	CTCTTC	2 cut(s)	526, 1962
BstACI	GRCGYC	1 cut(s)	312
BstAFI	CTTAAG	1 cut(s)	635
BstAUI	TGTACA	1 cut(s)	459
BstBI	TTCGAA	1 cut(s)	1835
BstC8I	GCNNGC	7 cut(s)	118, 130, 266, 649, 1197, 1408, 2219
BstDSI	CCRYGG	1 cut(s)	1266
BstEII	GGTNACC	3 cut(s)	492, 1688, 1841
BstF5I	GGATG	2 cut(s)	381, 2215
BstH2I	RGCGCY	1 cut(s)	1943
BstHHI	GCGC	2 cut(s)	1415, 1942
BstMAI	GTCTC	6 cut(s)	319, 510, 536, 1266, 1750, 1816
BstNI	CCWGG	6 cut(s)	539, 815, 1032, 1190, 1213, 1848
BstNSI	RCATGY	2 cut(s)	651, 2276
BstPI	GGTNACC	3 cut(s)	492, 1688, 1841
BstSFI	CTRYAG	1 cut(s)	966
BstSLI	GKGCMC	1 cut(s)	2127
BstX2I	RGATCY	6 cut(s)	556, 581, 733, 994, 1007, 1828
BstYI	RGATCY	6 cut(s)	556, 581, 733, 994, 1007, 1828
BstZ17I	GTATAC	1 cut(s)	1356
Bsu15I	ATCGAT	3 cut(s)	777, 1508, 1955
Bsu36I	CCTNAGG	1 cut(s)	375
BsuI	GTATCC	3 cut(s)	819, 884, 1785
BsuTUI	ATCGAT	3 cut(s)	777, 1508, 1955
BtgI	CCRYGG	1 cut(s)	1266
BtgZI	GCGATG	1 cut(s)	135
BtsCI	GGATG	2 cut(s)	381, 2215
BtsI	GCAGTG	4 cut(s)	387, 723, 1250, 2127
BtsIMutI	CAGTG	6 cut(s)	253, 387, 723, 1250, 1869, 2127
BveI	ACCTGC	1 cut(s)	1548
Cac8I	GCNNGC	7 cut(s)	118, 130, 266, 649, 1197, 1408, 2219
CfoI	GCGC	2 cut(s)	1415, 1942
Cfr10I	RCCGGY	2 cut(s)	106, 1406
Cfr13I	GGNCC	5 cut(s)	326, 1077, 1160, 1295, 1598
ClaI	ATCGAT	3 cut(s)	777, 1508, 1955
CseI	GACGC	2 cut(s)	301, 1097
CsiI	ACCWGGT	1 cut(s)	813
Csp6I	GTAC	6 cut(s)	460, 925, 1176, 1226, 1341, 1431
CviAII	CATG	9 cut(s)	648, 799, 1770, 1813, 1819, 1976, 2068, 2273, 2282
CviQI	GTAC	6 cut(s)	460, 925, 1176, 1226, 1341, 1431
DrdI	GACNNNNNNGTC	1 cut(s)	490
DseDI	GACNNNNNNGTC	1 cut(s)	490
EaeI	YGGCCR	2 cut(s)	1269, 1849
Eam1104I	CTCTTC	2 cut(s)	526, 1962
EarI	CTCTTC	2 cut(s)	526, 1962
EciI	GGCGGA	1 cut(s)	209
Eco130I	CCWWGG	2 cut(s)	171, 1281
Eco147I	AGGCCT	2 cut(s)	600, 1374
Eco31I	GGTCTC	2 cut(s)	510, 1816
Eco47I	GGWCC	2 cut(s)	1160, 1295
Eco81I	CCTNAGG	1 cut(s)	375
Eco88I	CYCGRG	1 cut(s)	568
Eco91I	GGTNACC	3 cut(s)	492, 1688, 1841
EcoO65I	GGTNACC	3 cut(s)	492, 1688, 1841
EcoRI	GAATTC	1 cut(s)	273
EcoRII	CCWGG	6 cut(s)	537, 813, 1030, 1188, 1211, 1846
EcoT14I	CCWWGG	2 cut(s)	171, 1281
ErhI	CCWWGG	2 cut(s)	171, 1281
Esp3I	CGTCTC	1 cut(s)	319
FaeI	CATG	9 cut(s)	651, 802, 1773, 1816, 1822, 1979, 2071, 2276, 2285
FalI	AAGNNNNNCTT	2 cut(s)	1035, 1067
FaqI	GGGAC	2 cut(s)	1281, 1566
FatI	CATG	9 cut(s)	647, 798, 1769, 1812, 1818, 1975, 2067, 2272, 2281
FauI	CCCGC	1 cut(s)	1745
FblI	GTMKAC	3 cut(s)	678, 1355, 1791
FokI	GGATG	2 cut(s)	368, 2222
FspBI	CTAG	6 cut(s)	129, 1112, 1196, 1904, 1931, 2036
GlaI	GCGC	2 cut(s)	1414, 1941
GsaI	CCCAGC	2 cut(s)	1137, 2153
GsuI	CTGGAG	2 cut(s)	879, 1644
HaeII	RGCGCY	1 cut(s)	1943
HgaI	GACGC	2 cut(s)	301, 1097
HhaI	GCGC	2 cut(s)	1415, 1942
Hin1I	GRCGYC	1 cut(s)	312
Hin1II	CATG	9 cut(s)	651, 802, 1773, 1816, 1822, 1979, 2071, 2276, 2285
Hin6I	GCGC	2 cut(s)	1413, 1940
HinP1I	GCGC	2 cut(s)	1413, 1940
HincII	GTYRAC	2 cut(s)	697, 820
HindII	GTYRAC	2 cut(s)	697, 820
HindIII	AAGCTT	3 cut(s)	632, 1154, 2290
HinfI	GANTC	4 cut(s)	544, 911, 1957, 2110
HpaI	GTTAAC	1 cut(s)	820
HphI	GGTGA	9 cut(s)	214, 253, 486, 563, 632, 1139, 1886, 2071, 2107
Hpy188I	TCNGA	6 cut(s)	298, 869, 1019, 1183, 1328, 2181
Hpy188III	TCNNGA	7 cut(s)	515, 568, 585, 737, 1526, 1623, 2170
Hpy99I	CGWCG	4 cut(s)	106, 326, 445, 617
HpyAV	CCTTC	7 cut(s)	166, 611, 860, 1331, 1842, 1927, 2155
HpyCH4III	ACNGT	5 cut(s)	248, 1241, 1841, 1882, 2095
HpyCH4IV	ACGT	4 cut(s)	167, 321, 2010, 2205
HpySE526I	ACGT	4 cut(s)	167, 321, 2010, 2205
Hsp92I	GRCGYC	1 cut(s)	312
Hsp92II	CATG	9 cut(s)	651, 802, 1773, 1816, 1822, 1979, 2071, 2276, 2285
HspAI	GCGC	2 cut(s)	1413, 1940
Kpn2I	TCCGGA	1 cut(s)	584
KroI	GCCGGC	1 cut(s)	1406
KroNI	GCCGGC	1 cut(s)	1408
KspAI	GTTAAC	1 cut(s)	820
LmnI	GCTCC	4 cut(s)	44, 359, 1192, 1709
LweI	GCATC	6 cut(s)	172, 1095, 1438, 1465, 2015, 2088
MabI	ACCWGGT	1 cut(s)	813
MaeI	CTAG	6 cut(s)	129, 1112, 1196, 1904, 1931, 2036
MaeII	ACGT	4 cut(s)	167, 321, 2010, 2205
MboII	GAAGA	6 cut(s)	543, 566, 1538, 1979, 2030, 2074
MfeI	CAATTG	1 cut(s)	682
MflI	RGATCY	6 cut(s)	556, 581, 733, 994, 1007, 1828
MhlI	GDGCHC	2 cut(s)	2127, 2179
MluCI	AATT	5 cut(s)	76, 273, 682, 1090, 1665
MlyI	GAGTC	2 cut(s)	538, 2119
MmeI	TCCRAC	2 cut(s)	972, 1110
MroI	TCCGGA	1 cut(s)	584
MroNI	GCCGGC	1 cut(s)	1406
MroXI	GAANNNNTTC	1 cut(s)	2165
MseI	TTAA	6 cut(s)	81, 504, 636, 711, 819, 2196
MslI	CAYNNNNRTG	2 cut(s)	711, 1817
MspCI	CTTAAG	1 cut(s)	635
MunI	CAATTG	1 cut(s)	682
MvaI	CCWGG	6 cut(s)	539, 815, 1032, 1190, 1213, 1848
NaeI	GCCGGC	1 cut(s)	1408
NciI	CCSGG	6 cut(s)	330, 357, 549, 1245, 1551, 1695
NgoMIV	GCCGGC	1 cut(s)	1406
NheI	GCTAGC	2 cut(s)	128, 1195
NlaIII	CATG	9 cut(s)	651, 802, 1773, 1816, 1822, 1979, 2071, 2276, 2285
NlaIV	GGNNCC	6 cut(s)	328, 334, 583, 1297, 1800, 1830
NmuCI	GTSAC	5 cut(s)	492, 620, 1054, 1982, 2001
NspI	RCATGY	2 cut(s)	651, 2276
NspV	TTCGAA	1 cut(s)	1835
PaeI	GCATGC	1 cut(s)	651
PaeR7I	CTCGAG	1 cut(s)	568
PceI	AGGCCT	2 cut(s)	600, 1374
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 1332
PdiI	GCCGGC	1 cut(s)	1408
PdmI	GAANNNNTTC	1 cut(s)	2165
PfeI	GAWTC	2 cut(s)	911, 1957
PfoI	TCCNGGA	3 cut(s)	355, 1243, 1549
PleI	GAGTC	2 cut(s)	538, 2118
PpsI	GAGTC	2 cut(s)	538, 2118
Psp6I	CCWGG	6 cut(s)	537, 813, 1030, 1188, 1211, 1846
PspEI	GGTNACC	3 cut(s)	492, 1688, 1841
PspFI	CCCAGC	2 cut(s)	1133, 2149
PspGI	CCWGG	6 cut(s)	537, 813, 1030, 1188, 1211, 1846
PspN4I	GGNNCC	6 cut(s)	328, 334, 583, 1297, 1800, 1830
PspPI	GGNCC	5 cut(s)	326, 1077, 1160, 1295, 1598
PsrI	GAACNNNNNNTAC	2 cut(s)	1782, 1814
PstI	CTGCAG	1 cut(s)	970
PsuI	RGATCY	6 cut(s)	556, 581, 733, 994, 1007, 1828
RsaI	GTAC	6 cut(s)	461, 926, 1177, 1227, 1342, 1432
RsaNI	GTAC	6 cut(s)	460, 925, 1176, 1226, 1341, 1431
RseI	CAYNNNNRTG	2 cut(s)	711, 1817
SaqAI	TTAA	6 cut(s)	81, 504, 636, 711, 819, 2196
Sau96I	GGNCC	5 cut(s)	326, 1077, 1160, 1295, 1598
ScaI	AGTACT	1 cut(s)	1227
SchI	GAGTC	2 cut(s)	538, 2119
SduI	GDGCHC	2 cut(s)	2127, 2179
SexAI	ACCWGGT	1 cut(s)	813
SfaNI	GCATC	6 cut(s)	172, 1095, 1438, 1465, 2015, 2088
SfcI	CTRYAG	1 cut(s)	966
Sfr274I	CTCGAG	1 cut(s)	568
SfuI	TTCGAA	1 cut(s)	1835
SinI	GGWCC	2 cut(s)	1160, 1295
SlaI	CTCGAG	1 cut(s)	568
SmiMI	CAYNNNNRTG	2 cut(s)	711, 1817
SmlI	CTYRAG	2 cut(s)	568, 635
SmoI	CTYRAG	2 cut(s)	568, 635
SpeI	ACTAGT	1 cut(s)	1930
SphI	GCATGC	1 cut(s)	651
Sse9I	AATT	5 cut(s)	76, 273, 682, 1090, 1665
SseBI	AGGCCT	2 cut(s)	600, 1374
SspI	AATATT	1 cut(s)	502
SspMI	CTAG	6 cut(s)	129, 1112, 1196, 1904, 1931, 2036
StuI	AGGCCT	2 cut(s)	600, 1374
StyI	CCWWGG	2 cut(s)	171, 1281
TaaI	ACNGT	5 cut(s)	248, 1241, 1841, 1882, 2095
TaiI	ACGT	4 cut(s)	170, 324, 2013, 2208
TasI	AATT	5 cut(s)	76, 273, 682, 1090, 1665
TatI	WGTACW	3 cut(s)	459, 924, 1225
TauI	GCSGC	3 cut(s)	211, 395, 2137
TfiI	GAWTC	2 cut(s)	911, 1957
Tru1I	TTAA	6 cut(s)	81, 504, 636, 711, 819, 2196
Tru9I	TTAA	6 cut(s)	81, 504, 636, 711, 819, 2196
TscAI	CASTG	6 cut(s)	253, 394, 723, 1257, 1876, 2127
TseFI	GTSAC	5 cut(s)	492, 620, 1054, 1982, 2001
Tsp45I	GTSAC	5 cut(s)	492, 620, 1054, 1982, 2001
TspDTI	ATGAA	2 cut(s)	1829, 2270
TspGWI	ACGGA	5 cut(s)	458, 549, 594, 917, 1073
TspRI	CASTG	6 cut(s)	253, 394, 723, 1257, 1876, 2127
Vha464I	CTTAAG	1 cut(s)	635
VneI	GTGCAC	1 cut(s)	2123
VpaK11BI	GGWCC	2 cut(s)	1160, 1295
XapI	RAATTY	2 cut(s)	76, 273
XceI	RCATGY	2 cut(s)	651, 2276
XcmI	CCANNNNNNNNNTGG	2 cut(s)	430, 1279
XhoI	CTCGAG	1 cut(s)	568
XmiI	GTMKAC	3 cut(s)	678, 1355, 1791
XmnI	GAANNNNTTC	1 cut(s)	2165
XspI	CTAG	6 cut(s)	129, 1112, 1196, 1904, 1931, 2036
ZrmI	AGTACT	1 cut(s)	1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.