Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003333

Physical Location & Seq

Genomic Coordinates Forward (+)

21024167 .. 21027772

Gene Structure 3606 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001804

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGTTATCTTTCTCTATGAGCTCAGTTTCCGGCCAGTCGTTTGCCTGCGGTAACGGTGCCAACAGCTTCCCGTTCTGCGACAAGTCATTGGAAGCCGATGCGCGGGTGGCGGACCTCGTGCAGAGGCTGACATTGCAGGAGAAGATTACGTTTCTGGTGAACAGTGCAGGCAGTGTGAGCAGGCTTGGAATACCCAAGTACGAGTGGTGGTCGGAGGCTTTACACGGTGTGTCGTACGTCGGCCCCGGGACCAAGTTTTCGAATGTCGTCCCCGGAGCTACGAGCTTCCCTCAGCCCATTTTGACCGCAGCGTCGTTCAACAACTCTCTCTACGAAGCCATCGGAAGGGTGGTGTCAACTGAGGCCAGAGCAATGTACAATGTGGGATTGGCAGGATTGACATTCTGGTCCCCAAACATTAACATTTTCCGAGACCCGAGATGGGGAAGAGGCCAAGAGACTCCGGGAGAAGACCCATTGCTCACGAGTAACTACGGATCAGGATACGTTAGAGGTCTTCAGCAAACCGACAGCGGAGACAAAAATAGGCTTAAAGTTGCAGCATGTTGTAAACATTATACAGCCTATGATTTGGACAATTGGAAAGGAACTGACAGATACCATTTCAATGCAGTGGTAACAAAACAAGACATGGATGATACATTTCAGCCACCATTCAAAAGTTGTGTTCTTGATGGGAATGTTGCTAGTGTCATGTGTTCTTACAACCAGGTTAATGGAATACCAACCTGCGCAGACCCAAACCTTCTTTCTGGGGTTATCCGAGGAGAATGGAAGTTGAATGGATACATAGTCACTGATTGTGATTCAATAGATGTGTACTACAAGAACCAACACTACACCAAGACACCAGAAGAGGCTGCAGCTAAGGCTATATTAGCAGGTTTGGATCTAAACTGTGGATCATTCTTAGGACAGTACACAGAAGGAGCAGTGAAAGGAGGACTTGTAACTGAGGCAGCCATTGACAAGGCCATCACAAACAATTTTGGCACATTGATGCGTTTAGGATTCTTCGATGGTGATCCAAGCAAGCAACCATATGGAGATCTCGGTCCAAAGGATGTGTGCACTCCACAGAACCAGGAGCTGGCACGTGAAACAGCAAGACAAGGGATAGTGTTGCTCAAGAACACTCCAGGATCACTGCCTCTATCTCCCACAGCCATCAAATCCTTGGCAGTCATAGGTCCCAATGGCAATGTCACAAAGACTATGATTGGCAACTACGAAGGCACCCCATGCAAATATACAACTCCTTTGCAAGGCCTAACAGCTGTAGTTCCCACAACATTTGTGGCCGGGTGCGTTGATGTGGCTTGTGGCACTGCCCAGGTAGATGATGCTACCAAGTTAGCAGCCGCAGCAGATGCAACTGTACTTATAGTGGGTGAAAATCAATCAATTGAGACTGAAAGTCATGATAGAATCGACCTCCATCTTCCAGGACAGCAAACTCTTCTAATAACTGAAGTAGCGAAGGCTTCTAAAGGACCTGTAATCCTTGTCATAATGTCTGGAGGAGGGTTCGATATTACCTTTGCAAAAAATGATGATAAAATTACAAGCATTCTATGGGTTGGTTACCCTGGCGAAGCTGGTGGAGCTGCCATTGCTGACGTGATTTTTGGCTTTTATAATCCAAGTGGAAGACTACCTATGACGTGGTATCCTCAAGCTTATGTAGACAAGGTCCCTATGACAAACATGAATATGAGACCAGATCCTGCAAGTGGTTACCCGGGCAGGACTTACCGGTTTTACACTGGTGACACGGTTTATGAATTTGGATACGGAATAAGCTACTCCACCTTCAATCACCGCCTGGTTCGAGCACCGAAGCAAGTTTCCATTCCCTTAGAGGAGGATCATGTATGCCACACATCAAGTTGCCAATCACTTGATGTTGTAGAAGAAAGGTGTCAAAATCTAGCTTTCGATATCCATTTGGGGGTGAAAAACATGGGCAAAATGAGTGGAAGTCATACAGTCTTATTGTATTCGAGTCCTCCATCTGTGCACAATTCTCCTAAAAAGCACTTGTTGGGATTTGAGAAGGTGTTCCTTAGCTCTCAGGGAGAAGCATTGGTCAAGTTCAATGTGGATGTTTGCAAGCACTTGAGTGTGGTTGATGAGCTAGGAAGTAGGAAAGTTGCTTTGGGAGATCATGTACTACATGTGGGAGACTTGAAACAGACATTGAGTGTGAGGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0031221 GO:0031222 GO:0043170 GO:0043207 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044464 GO:0045087 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:0098542 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

746

Amino Acids

80.54

Weight (kDa)

5.92

Isoelectric Point (pI)

30.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	90 - 333	7.9e-34	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	380 - 609	7.1e-51	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	669 - 736	7.1e-10	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09730 AT5G64570 AT5G64570 AT5G64570
fragaria_vesca	FvH4_5g30690 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710
malus_domestica	MD08G1221800.v1.1 MD15G1414100.v1.1
prunus_persica	Prupe.1G559400_v2.0.a1 Prupe.1G559400_v2.0.a1
pyrus_communis	pycom08g19240 pycom15g36840
rosa_chinensis	RchiOBHm_Chr7g0225551 RchiOBHm_Chr7g0225581
rosa_laevigata	RLG00000001804 RLG00000001806
rosa_multiflora	Rmu_co8377913.1_g000001 Rmu_sc0003876.1_g000021 Rmu_sc0006321.1_g000006
rosa_roxburghii	Rroxscaffold_3G00233670 Rroxscaffold_3G00233690
rosa_rugosa	Rorug07G0231600 Rorug07G0231800
rosa_samantha	Rh7AG372300 Rh7AG372500 Rh7BG365400 Rh7BG365500 Rh7CG390600 Rh7CG390800 Rh7DG376200 Rh7DG376300
rosa_wichuraiana	Rw7G031750 Rw7G031760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1659
AasI	GACNNNNNNGTC	2 cut(s)	310, 406
Acc16I	TGCGCA	1 cut(s)	754
Acc36I	ACCTGC	2 cut(s)	758, 893
AccB1I	GGYRCC	2 cut(s)	56, 1256
AccB7I	CCANNNNNTGG	1 cut(s)	1111
AccI	GTMKAC	1 cut(s)	1707
AccII	CGCG	1 cut(s)	103
AciI	CCGC	7 cut(s)	48, 103, 110, 306, 534, 1383, 1843
AclWI	GGATC	7 cut(s)	505, 918, 931, 1040, 1171, 1739, 1896
AcoI	YGGCCR	2 cut(s)	31, 1320
AcsI	RAATTY	1 cut(s)	1805
AcuI	CTGAAG	2 cut(s)	503, 1512
AcvI	CACGTG	1 cut(s)	1118
AdeI	CACNNNGTG	1 cut(s)	229
AfaI	GTAC	7 cut(s)	200, 236, 377, 842, 941, 1401, 2193
AfiI	CCNNNNNNNGG	8 cut(s)	102, 345, 442, 443, 1111, 1286, 1754, 1972
AflIII	ACRYGT	1 cut(s)	2197
AgeI	ACCGGT	1 cut(s)	1776
AgsI	TTSAA	8 cut(s)	319, 628, 679, 802, 831, 1837, 2119, 2212
AhdI	GACNNNNNGTC	1 cut(s)	1437
AjiI	CACGTC	2 cut(s)	1642, 1686
AjnI	CCWGG	7 cut(s)	729, 1104, 1159, 1353, 1465, 1609, 1845
AjuI	GAANNNNNNNTTGG	4 cut(s)	754, 786, 2162, 2194
AleI	CACNNNNGTG	1 cut(s)	2142
Alw21I	GWGCWC	4 cut(s)	23, 1094, 1858, 2043
Alw26I	GTCTC	6 cut(s)	426, 452, 531, 1424, 1732, 2199
Alw44I	GTGCAC	2 cut(s)	1090, 2039
AlwI	GGATC	7 cut(s)	505, 918, 931, 1040, 1171, 1739, 1896
AlwNI	CAGNNNCTG	3 cut(s)	127, 1111, 1748
Ama87I	CYCGRG	3 cut(s)	245, 436, 1762
AoxI	GGCC	7 cut(s)	31, 241, 363, 451, 993, 1288, 1320
ApaLI	GTGCAC	2 cut(s)	1090, 2039
ApeKI	GCWGC	8 cut(s)	308, 560, 881, 884, 980, 1379, 1385, 1628
ApoI	RAATTY	1 cut(s)	1805
ArsI	GACNNNNNNTTYG	4 cut(s)	241, 273, 1631, 1663
AsiGI	ACCGGT	1 cut(s)	1776
Asp700I	GAANNNNTTC	1 cut(s)	2081
AspLEI	GCGC	2 cut(s)	103, 755
AspS9I	GGNCC	8 cut(s)	112, 242, 249, 408, 1076, 1211, 1514, 1714
AsuC2I	CCSGG	7 cut(s)	246, 247, 273, 465, 1324, 1763, 1764
AsuHPI	GGTGA	6 cut(s)	169, 1055, 1424, 1802, 1832, 1987
AsuII	TTCGAA	1 cut(s)	260
AvaI	CYCGRG	3 cut(s)	245, 436, 1762
AvaII	GGWCC	7 cut(s)	112, 249, 408, 1076, 1211, 1514, 1714
BaeGI	GKGCMC	2 cut(s)	1094, 2043
BanI	GGYRCC	2 cut(s)	56, 1256
BanII	GRGCYC	1 cut(s)	23
BauI	CACGAG	2 cut(s)	116, 484
BbrPI	CACGTG	1 cut(s)	1118
BbsI	GAAGAC	3 cut(s)	477, 509, 1678
Bbv12I	GWGCWC	4 cut(s)	23, 1094, 1858, 2043
BbvCI	CCTCAGC	1 cut(s)	291
BbvI	GCAGC	8 cut(s)	320, 572, 868, 896, 992, 1391, 1397, 1615
BccI	CCATC	8 cut(s)	347, 435, 689, 1004, 1034, 1196, 1467, 2041
BciT130I	CCWGG	7 cut(s)	731, 1106, 1161, 1355, 1467, 1611, 1847
BciVI	GTATCC	4 cut(s)	497, 800, 1701, 1805
BcnI	CCSGG	7 cut(s)	246, 247, 273, 465, 1324, 1763, 1764
BcoDI	GTCTC	6 cut(s)	426, 452, 531, 1424, 1732, 2199
BfaI	CTAG	3 cut(s)	708, 1952, 2159
BfmI	CTRYAG	2 cut(s)	882, 1299
BfuAI	ACCTGC	2 cut(s)	758, 893
BfuI	GTATCC	4 cut(s)	497, 800, 1701, 1805
BglII	AGATCT	1 cut(s)	1069
BisI	GCNGC	9 cut(s)	309, 561, 882, 885, 981, 1380, 1383, 1386, 1629
BlsI	GCNGC	9 cut(s)	310, 562, 883, 886, 982, 1381, 1384, 1387, 1630
Bme18I	GGWCC	7 cut(s)	112, 249, 408, 1076, 1211, 1514, 1714
BmeRI	GACNNNNNGTC	1 cut(s)	1437
BmeT110I	CYCGRG	3 cut(s)	245, 436, 1762
BmgBI	CACGTC	2 cut(s)	1642, 1686
BmgT120I	GGNCC	8 cut(s)	112, 242, 249, 408, 1076, 1211, 1514, 1714
BmiI	GGNNCC	7 cut(s)	58, 244, 250, 410, 1213, 1258, 1716
BmsI	GCATC	4 cut(s)	88, 1011, 1354, 1381
BpiI	GAAGAC	3 cut(s)	477, 509, 1678
BplI	GAGNNNNNCTC	2 cut(s)	274, 306
BpmI	CTGGAG	2 cut(s)	1143, 1560
Bpu10I	CCTNAGC	3 cut(s)	291, 888, 2087
Bpu14I	TTCGAA	1 cut(s)	260
BpuEI	CTTGAG	3 cut(s)	1133, 1680, 2161
BpuMI	CCSGG	7 cut(s)	246, 247, 273, 465, 1324, 1763, 1764
BsaAI	YACGTR	1 cut(s)	1118
BsaBI	GATNNNNATC	1 cut(s)	1044
BsaI	GGTCTC	2 cut(s)	426, 1732
BsaJI	CCNNGG	8 cut(s)	244, 245, 271, 784, 1197, 1353, 1609, 1762
BsaWI	WCCGGW	1 cut(s)	1776
BsaXI	ACNNNNNCTCC	4 cut(s)	954, 984, 2175, 2205
Bsc4I	CCNNNNNNNGG	8 cut(s)	102, 345, 442, 443, 1111, 1286, 1754, 1972
Bse118I	RCCGGY	1 cut(s)	1776
Bse1I	ACTGG	2 cut(s)	34, 1792
Bse3DI	GCAATG	5 cut(s)	131, 378, 476, 1228, 1632
Bse8I	GATNNNNATC	1 cut(s)	1044
BseBI	CCWGG	7 cut(s)	731, 1106, 1161, 1355, 1467, 1611, 1847
BseDI	CCNNGG	8 cut(s)	244, 245, 271, 784, 1197, 1353, 1609, 1762
BseGI	GGATG	3 cut(s)	661, 1090, 2131
BseJI	GATNNNNATC	1 cut(s)	1044
BseLI	CCNNNNNNNGG	8 cut(s)	102, 345, 442, 443, 1111, 1286, 1754, 1972
BseMI	GCAATG	5 cut(s)	131, 378, 476, 1228, 1632
BseMII	CTCAG	5 cut(s)	36, 305, 351, 966, 2108
BseNI	ACTGG	2 cut(s)	34, 1792
BseRI	GAGGAG	3 cut(s)	801, 1557, 1898
BseSI	GKGCMC	2 cut(s)	1094, 2043
BseXI	GCAGC	8 cut(s)	320, 572, 868, 896, 992, 1391, 1397, 1615
BsgI	GTGCAG	2 cut(s)	140, 186
Bsh1236I	CGCG	1 cut(s)	103
BshFI	GGCC	7 cut(s)	33, 243, 365, 453, 995, 1290, 1322
BshNI	GGYRCC	2 cut(s)	56, 1256
BshTI	ACCGGT	1 cut(s)	1776
BsiHKAI	GWGCWC	4 cut(s)	23, 1094, 1858, 2043
BsiHKCI	CYCGRG	3 cut(s)	245, 436, 1762
BsiSI	CCGG	7 cut(s)	30, 246, 273, 464, 1323, 1763, 1777
BsiWI	CGTACG	1 cut(s)	234
BslFI	GGGAC	5 cut(s)	254, 262, 394, 1197, 1700
BslI	CCNNNNNNNGG	8 cut(s)	102, 345, 442, 443, 1111, 1286, 1754, 1972
BsmAI	GTCTC	6 cut(s)	426, 452, 531, 1424, 1732, 2199
BsmFI	GGGAC	5 cut(s)	254, 262, 394, 1197, 1700
BsmI	GAATGC	1 cut(s)	1590
BsnI	GGCC	7 cut(s)	33, 243, 365, 453, 995, 1290, 1322
Bso31I	GGTCTC	2 cut(s)	426, 1732
BsoBI	CYCGRG	3 cut(s)	245, 436, 1762
Bsp119I	TTCGAA	1 cut(s)	260
Bsp1286I	GDGCHC	4 cut(s)	23, 1094, 1858, 2043
Bsp1407I	TGTACA	1 cut(s)	375
Bsp143I	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
BspACI	CCGC	7 cut(s)	48, 103, 110, 306, 534, 1383, 1843
BspANI	GGCC	7 cut(s)	33, 243, 365, 453, 995, 1290, 1322
BspCNI	CTCAG	5 cut(s)	35, 304, 352, 967, 2107
BspFNI	CGCG	1 cut(s)	103
BspHI	TCATGA	1 cut(s)	1441
BspLI	GGNNCC	7 cut(s)	58, 244, 250, 410, 1213, 1258, 1716
BspMAI	CTGCAG	1 cut(s)	886
BspMI	ACCTGC	2 cut(s)	758, 893
BspPI	GGATC	7 cut(s)	505, 918, 931, 1040, 1171, 1739, 1896
BspT104I	TTCGAA	1 cut(s)	260
BspT107I	GGYRCC	2 cut(s)	56, 1256
BspTNI	GGTCTC	2 cut(s)	426, 1732
BsrDI	GCAATG	5 cut(s)	131, 378, 476, 1228, 1632
BsrFI	RCCGGY	1 cut(s)	1776
BsrGI	TGTACA	1 cut(s)	375
BsrI	ACTGG	2 cut(s)	34, 1792
BssAI	RCCGGY	1 cut(s)	1776
BssECI	CCNNGG	8 cut(s)	244, 245, 271, 784, 1197, 1353, 1609, 1762
BssMI	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
BssSI	CACGAG	2 cut(s)	116, 484
BssT1I	CCWWGG	1 cut(s)	1197
Bst2BI	CACGAG	2 cut(s)	116, 484
Bst2UI	CCWGG	7 cut(s)	731, 1106, 1161, 1355, 1467, 1611, 1847
Bst4CI	ACNGT	8 cut(s)	56, 164, 227, 920, 939, 1399, 1798, 2011
Bst6I	CTCTTC	3 cut(s)	442, 870, 1485
BstAPI	GCANNNNNTGC	2 cut(s)	1263, 1391
BstAUI	TGTACA	1 cut(s)	375
BstBAI	YACGTR	1 cut(s)	1118
BstBI	TTCGAA	1 cut(s)	260
BstC8I	GCNNGC	6 cut(s)	46, 169, 182, 1055, 1113, 2135
BstDEI	CTNAG	9 cut(s)	22, 291, 360, 888, 931, 975, 1879, 2087, 2094
BstEII	GGTNACC	2 cut(s)	1604, 1757
BstENI	CCTNNNNNAGG	1 cut(s)	1284
BstF5I	GGATG	3 cut(s)	661, 1090, 2131
BstFNI	CGCG	1 cut(s)	103
BstHHI	GCGC	2 cut(s)	103, 755
BstKTI	GATC	9 cut(s)	500, 913, 926, 1048, 1072, 1166, 1747, 1891, 2188
BstMAI	GTCTC	6 cut(s)	426, 452, 531, 1424, 1732, 2199
BstMBI	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
BstNI	CCWGG	7 cut(s)	731, 1106, 1161, 1355, 1467, 1611, 1847
BstNSI	RCATGY	2 cut(s)	567, 2201
BstPI	GGTNACC	2 cut(s)	1604, 1757
BstSFI	CTRYAG	2 cut(s)	882, 1299
BstSLI	GKGCMC	2 cut(s)	1094, 2043
BstUI	CGCG	1 cut(s)	103
BstV1I	GCAGC	8 cut(s)	320, 572, 868, 896, 992, 1391, 1397, 1615
BstV2I	GAAGAC	3 cut(s)	477, 509, 1678
BstX2I	RGATCY	3 cut(s)	910, 1069, 1744
BstXI	CCANNNNNNTGG	1 cut(s)	737
BstYI	RGATCY	3 cut(s)	910, 1069, 1744
BsuI	GTATCC	4 cut(s)	497, 800, 1701, 1805
BsuRI	GGCC	7 cut(s)	33, 243, 365, 453, 995, 1290, 1322
BtrI	CACGTC	2 cut(s)	1642, 1686
BtsCI	GGATG	3 cut(s)	661, 1090, 2131
BtsI	GCAGTG	5 cut(s)	178, 639, 960, 1166, 1347
BtsIMutI	CAGTG	8 cut(s)	169, 178, 639, 816, 960, 1166, 1347, 1785
BveI	ACCTGC	2 cut(s)	758, 893
Cac8I	GCNNGC	6 cut(s)	46, 169, 182, 1055, 1113, 2135
CaiI	CAGNNNCTG	3 cut(s)	127, 1111, 1748
CciI	TCATGA	1 cut(s)	1441
CfoI	GCGC	2 cut(s)	103, 755
Cfr10I	RCCGGY	1 cut(s)	1776
Cfr13I	GGNCC	8 cut(s)	112, 242, 249, 408, 1076, 1211, 1514, 1714
Cfr9I	CCCGGG	2 cut(s)	245, 1762
CseI	GACGC	1 cut(s)	300
CsiI	ACCWGGT	1 cut(s)	729
Csp6I	GTAC	7 cut(s)	199, 235, 376, 841, 940, 1400, 2192
CspAI	ACCGGT	1 cut(s)	1776
CspCI	CAANNNNNGTGG	6 cut(s)	1297, 1299, 1332, 1334, 1978, 2013
CviQI	GTAC	7 cut(s)	199, 235, 376, 841, 940, 1400, 2192
DdeI	CTNAG	9 cut(s)	22, 291, 360, 888, 931, 975, 1879, 2087, 2094
DpnI	GATC	9 cut(s)	499, 912, 925, 1047, 1071, 1165, 1746, 1890, 2187
DpnII	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
DraIII	CACNNNGTG	1 cut(s)	229
DrdI	GACNNNNNNGTC	2 cut(s)	310, 406
DriI	GACNNNNNGTC	1 cut(s)	1437
DseDI	GACNNNNNNGTC	2 cut(s)	310, 406
EaeI	YGGCCR	2 cut(s)	31, 1320
Eam1104I	CTCTTC	3 cut(s)	442, 870, 1485
Eam1105I	GACNNNNNGTC	1 cut(s)	1437
EarI	CTCTTC	3 cut(s)	442, 870, 1485
EciI	GGCGGA	1 cut(s)	125
Ecl136II	GAGCTC	1 cut(s)	21
Eco130I	CCWWGG	1 cut(s)	1197
Eco147I	AGGCCT	1 cut(s)	1290
Eco24I	GRGCYC	1 cut(s)	23
Eco31I	GGTCTC	2 cut(s)	426, 1732
Eco32I	GATATC	1 cut(s)	1963
Eco47I	GGWCC	7 cut(s)	112, 249, 408, 1076, 1211, 1514, 1714
Eco53kI	GAGCTC	1 cut(s)	21
Eco57I	CTGAAG	2 cut(s)	503, 1512
Eco72I	CACGTG	1 cut(s)	1118
Eco88I	CYCGRG	3 cut(s)	245, 436, 1762
Eco91I	GGTNACC	2 cut(s)	1604, 1757
EcoICRI	GAGCTC	1 cut(s)	21
EcoNI	CCTNNNNNAGG	1 cut(s)	1284
EcoO109I	RGGNCCY	3 cut(s)	1211, 1514, 1714
EcoO65I	GGTNACC	2 cut(s)	1604, 1757
EcoRII	CCWGG	7 cut(s)	729, 1104, 1159, 1353, 1465, 1609, 1845
EcoRV	GATATC	1 cut(s)	1963
EcoT14I	CCWWGG	1 cut(s)	1197
EcoT38I	GRGCYC	1 cut(s)	23
ErhI	CCWWGG	1 cut(s)	1197
FalI	AAGNNNNNCTT	2 cut(s)	951, 983
FaqI	GGGAC	5 cut(s)	254, 262, 394, 1197, 1700
FauI	CCCGC	1 cut(s)	96
FauNDI	CATATG	1 cut(s)	1063
FblI	GTMKAC	1 cut(s)	1707
Fnu4HI	GCNGC	9 cut(s)	309, 561, 882, 885, 981, 1380, 1383, 1386, 1629
FokI	GGATG	3 cut(s)	668, 1097, 2138
FriOI	GRGCYC	1 cut(s)	23
Fsp4HI	GCNGC	9 cut(s)	309, 561, 882, 885, 981, 1380, 1383, 1386, 1629
FspBI	CTAG	3 cut(s)	708, 1952, 2159
FspI	TGCGCA	1 cut(s)	754
GlaI	GCGC	2 cut(s)	102, 754
GluI	GCNGC	9 cut(s)	309, 561, 882, 885, 981, 1380, 1383, 1386, 1629
GsuI	CTGGAG	2 cut(s)	1143, 1560
HaeIII	GGCC	7 cut(s)	33, 243, 365, 453, 995, 1290, 1322
HapII	CCGG	7 cut(s)	30, 246, 273, 464, 1323, 1763, 1777
HgaI	GACGC	1 cut(s)	300
HhaI	GCGC	2 cut(s)	103, 755
Hin6I	GCGC	2 cut(s)	101, 753
HinP1I	GCGC	2 cut(s)	101, 753
HincII	GTYRAC	1 cut(s)	357
HindII	GTYRAC	1 cut(s)	357
HindIII	AAGCTT	1 cut(s)	1698
HinfI	GANTC	5 cut(s)	460, 827, 1032, 1449, 2026
HpaII	CCGG	7 cut(s)	30, 246, 273, 464, 1323, 1763, 1777
HphI	GGTGA	6 cut(s)	169, 1055, 1424, 1802, 1832, 1987
Hpy166II	GTNNAC	8 cut(s)	160, 357, 572, 841, 942, 1092, 1708, 2041
Hpy188I	TCNGA	4 cut(s)	214, 344, 431, 785
Hpy188III	TCNNGA	6 cut(s)	484, 501, 692, 1150, 1442, 1539
Hpy8I	GTNNAC	8 cut(s)	160, 357, 572, 841, 942, 1092, 1708, 2041
Hpy99I	CGWCG	2 cut(s)	242, 316
HpyAV	CCTTC	7 cut(s)	339, 776, 941, 1247, 1495, 1843, 2071
HpyCH4III	ACNGT	8 cut(s)	56, 164, 227, 920, 939, 1399, 1798, 2011
HpyCH4IV	ACGT	6 cut(s)	149, 237, 507, 1117, 1641, 1685
HpyF3I	CTNAG	9 cut(s)	22, 291, 360, 888, 931, 975, 1879, 2087, 2094
HpySE526I	ACGT	6 cut(s)	149, 237, 507, 1117, 1641, 1685
HspAI	GCGC	2 cut(s)	101, 753
Kzo9I	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
LmnI	GCTCC	4 cut(s)	275, 950, 1108, 1625
Lsp1109I	GCAGC	8 cut(s)	320, 572, 868, 896, 992, 1391, 1397, 1615
LweI	GCATC	4 cut(s)	88, 1011, 1354, 1381
MabI	ACCWGGT	1 cut(s)	729
MaeI	CTAG	3 cut(s)	708, 1952, 2159
MaeII	ACGT	6 cut(s)	149, 237, 507, 1117, 1641, 1685
MaeIII	GTNAC	9 cut(s)	50, 488, 637, 814, 970, 1225, 1604, 1757, 1790
MalI	GATC	9 cut(s)	499, 912, 925, 1047, 1071, 1165, 1746, 1890, 2187
MboI	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
MfeI	CAATTG	2 cut(s)	598, 1425
MflI	RGATCY	3 cut(s)	910, 1069, 1744
MhlI	GDGCHC	4 cut(s)	23, 1094, 1858, 2043
MluCI	AATT	6 cut(s)	598, 1006, 1425, 1581, 1805, 2044
MlyI	GAGTC	2 cut(s)	454, 2035
MmeI	TCCRAC	1 cut(s)	192
MroXI	GAANNNNTTC	1 cut(s)	2081
MseI	TTAA	3 cut(s)	420, 552, 735
MslI	CAYNNNNRTG	4 cut(s)	627, 1019, 1733, 2142
MspA1I	CMGCKG	2 cut(s)	534, 1298
MspI	CCGG	7 cut(s)	30, 246, 273, 464, 1323, 1763, 1777
MunI	CAATTG	2 cut(s)	598, 1425
Mva1269I	GAATGC	1 cut(s)	1590
MvaI	CCWGG	7 cut(s)	731, 1106, 1161, 1355, 1467, 1611, 1847
MvnI	CGCG	1 cut(s)	103
NciI	CCSGG	7 cut(s)	246, 247, 273, 465, 1324, 1763, 1764
NdeI	CATATG	1 cut(s)	1063
NdeII	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
NlaIV	GGNNCC	7 cut(s)	58, 244, 250, 410, 1213, 1258, 1716
NmuCI	GTSAC	3 cut(s)	814, 1225, 1790
NsbI	TGCGCA	1 cut(s)	754
NspI	RCATGY	2 cut(s)	567, 2201
NspV	TTCGAA	1 cut(s)	260
OliI	CACNNNNGTG	1 cut(s)	2142
PagI	TCATGA	1 cut(s)	1441
PceI	AGGCCT	1 cut(s)	1290
PciI	ACATGT	1 cut(s)	2197
PctI	GAATGC	1 cut(s)	1590
PdmI	GAANNNNTTC	1 cut(s)	2081
PfeI	GAWTC	3 cut(s)	827, 1032, 1449
Pfl23II	CGTACG	1 cut(s)	234
PflFI	GACNNNGTC	1 cut(s)	1712
PflMI	CCANNNNNTGG	1 cut(s)	1111
PfoI	TCCNGGA	3 cut(s)	463, 1159, 1465
PinAI	ACCGGT	1 cut(s)	1776
PkrI	GCNGC	9 cut(s)	310, 562, 883, 886, 982, 1381, 1384, 1387, 1630
PleI	GAGTC	2 cut(s)	454, 2034
PmaCI	CACGTG	1 cut(s)	1118
PmlI	CACGTG	1 cut(s)	1118
PpsI	GAGTC	2 cut(s)	454, 2034
Ppu21I	YACGTR	1 cut(s)	1118
PpuMI	RGGWCCY	3 cut(s)	1211, 1514, 1714
PscI	ACATGT	1 cut(s)	2197
PsiI	TTATAA	1 cut(s)	1659
Psp124BI	GAGCTC	1 cut(s)	23
Psp5II	RGGWCCY	3 cut(s)	1211, 1514, 1714
Psp6I	CCWGG	7 cut(s)	729, 1104, 1159, 1353, 1465, 1609, 1845
PspCI	CACGTG	1 cut(s)	1118
PspEI	GGTNACC	2 cut(s)	1604, 1757
PspGI	CCWGG	7 cut(s)	729, 1104, 1159, 1353, 1465, 1609, 1845
PspLI	CGTACG	1 cut(s)	234
PspN4I	GGNNCC	7 cut(s)	58, 244, 250, 410, 1213, 1258, 1716
PspPI	GGNCC	8 cut(s)	112, 242, 249, 408, 1076, 1211, 1514, 1714
PspPPI	RGGWCCY	3 cut(s)	1211, 1514, 1714
PsrI	GAACNNNNNNTAC	2 cut(s)	842, 874
PstI	CTGCAG	1 cut(s)	886
PstNI	CAGNNNCTG	3 cut(s)	127, 1111, 1748
PsuI	RGATCY	3 cut(s)	910, 1069, 1744
PsyI	GACNNNGTC	1 cut(s)	1712
PvuII	CAGCTG	1 cut(s)	1298
RsaI	GTAC	7 cut(s)	200, 236, 377, 842, 941, 1401, 2193
RsaNI	GTAC	7 cut(s)	199, 235, 376, 841, 940, 1400, 2192
RseI	CAYNNNNRTG	4 cut(s)	627, 1019, 1733, 2142
SacI	GAGCTC	1 cut(s)	23
SaqAI	TTAA	3 cut(s)	420, 552, 735
SatI	GCNGC	9 cut(s)	309, 561, 882, 885, 981, 1380, 1383, 1386, 1629
Sau3AI	GATC	9 cut(s)	497, 910, 923, 1045, 1069, 1163, 1744, 1888, 2185
Sau96I	GGNCC	8 cut(s)	112, 242, 249, 408, 1076, 1211, 1514, 1714
SchI	GAGTC	2 cut(s)	454, 2035
SduI	GDGCHC	4 cut(s)	23, 1094, 1858, 2043
SexAI	ACCWGGT	1 cut(s)	729
SfaNI	GCATC	4 cut(s)	88, 1011, 1354, 1381
SfcI	CTRYAG	2 cut(s)	882, 1299
SfuI	TTCGAA	1 cut(s)	260
SinI	GGWCC	7 cut(s)	112, 249, 408, 1076, 1211, 1514, 1714
SmaI	CCCGGG	2 cut(s)	247, 1764
SmiMI	CAYNNNNRTG	4 cut(s)	627, 1019, 1733, 2142
SmlI	CTYRAG	3 cut(s)	1148, 1695, 2140
SmoI	CTYRAG	3 cut(s)	1148, 1695, 2140
Sse9I	AATT	6 cut(s)	598, 1006, 1425, 1581, 1805, 2044
SseBI	AGGCCT	1 cut(s)	1290
SsiI	CCGC	7 cut(s)	48, 103, 110, 306, 534, 1383, 1843
SspMI	CTAG	3 cut(s)	708, 1952, 2159
SstI	GAGCTC	1 cut(s)	23
StuI	AGGCCT	1 cut(s)	1290
StyI	CCWWGG	1 cut(s)	1197
TaaI	ACNGT	8 cut(s)	56, 164, 227, 920, 939, 1399, 1798, 2011
TaiI	ACGT	6 cut(s)	152, 240, 510, 1120, 1644, 1688
TaqI	TCGA	7 cut(s)	260, 1038, 1452, 1551, 1852, 1959, 2024
TaqII	GACCGA	1 cut(s)	1064
TasI	AATT	6 cut(s)	598, 1006, 1425, 1581, 1805, 2044
TatI	WGTACW	5 cut(s)	375, 840, 939, 1399, 2191
TauI	GCSGC	1 cut(s)	1385
TfiI	GAWTC	3 cut(s)	827, 1032, 1449
Tru1I	TTAA	3 cut(s)	420, 552, 735
Tru9I	TTAA	3 cut(s)	420, 552, 735
TscAI	CASTG	8 cut(s)	169, 178, 639, 823, 960, 1173, 1354, 1792
TseFI	GTSAC	3 cut(s)	814, 1225, 1790
TseI	GCWGC	8 cut(s)	308, 560, 881, 884, 980, 1379, 1385, 1628
Tsp45I	GTSAC	3 cut(s)	814, 1225, 1790
TspDTI	ATGAA	2 cut(s)	1745, 1818
TspGWI	ACGGA	2 cut(s)	510, 1830
TspMI	CCCGGG	2 cut(s)	245, 1762
TspRI	CASTG	8 cut(s)	169, 178, 639, 823, 960, 1173, 1354, 1792
Tth111I	GACNNNGTC	1 cut(s)	1712
Van91I	CCANNNNNTGG	1 cut(s)	1111
VneI	GTGCAC	2 cut(s)	1090, 2039
VpaK11BI	GGWCC	7 cut(s)	112, 249, 408, 1076, 1211, 1514, 1714
XagI	CCTNNNNNAGG	1 cut(s)	1284
XapI	RAATTY	1 cut(s)	1805
XceI	RCATGY	2 cut(s)	567, 2201
XcmI	CCANNNNNNNNNTGG	3 cut(s)	346, 1195, 1315
XmaI	CCCGGG	2 cut(s)	245, 1762
XmiI	GTMKAC	1 cut(s)	1707
XmnI	GAANNNNTTC	1 cut(s)	2081
XspI	CTAG	3 cut(s)	708, 1952, 2159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.