Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003333

Physical Location & Seq

Genomic Coordinates Forward (+)

51376753 .. 51380967

Gene Structure 4215 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1414100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGGCTTCCTCTATCATTCGGTTCTCTGTTTTCCTTTGCTCCTTTTTGTTTCAATACTTGTTATTCTTTTCGCAAAATTTGAAGGGCGTTTTAGGCCAATCGTCACCGGTTTTCGCTTGCAATGTCGCTAGCAACGCCACCCTATCGAGCTTCGCCTTCTGCGACACATCGCTGGGGGTCGATGCGAGAGTGGCGGACTTGGTGAAGCGGCTGACATTACCAGAGAAAATCGGGTTTTTGGTGAACAGTGCAGGGAGTGTGAGCAGGCTTGGAATTCCCAAATATGAGTGGTGGTCGGAGGCATTACACGGCGTGTCATACGTCGGCCCTGGCACCAAGTTTTCGAGTCTCGTTCCCGGAGCTACCAGCTTCCCTCAGGTCATCCTCACTGCCGCTTCTTTCAATACTTCTCTTTTTGAAGCCATTGGCAGGGTGGTTTCGACGGAGGCCAGAGCAATGTACAATGTGGGATTGGCAGGACTGACATATTGGTCACCAAATATTAACATATTTCGAGACCCCAGATGGGGAAGAGGCCAGGAGACTCCGGGTGAAGACCCGTTGCTCTCGAGTAAATACGGATCCGGATATGTTCGAGGCCTTCAACAAACCGACGATGGTGATAAGAATAAGCTTAAGGTTGCTGCATGCTGCAAACATTATACAGCCTATGATATAGACAATTGGAAAGGGGTTGACAGATACCATTTTAATGCAGTGGTGACGAAACAAGATATGGACGACACATTTCAACCGCCGTTCAAAAGTTGTGTTATCGATGGCAATGTTGCGAGTGTCATGTGTTCTTACAACCAGGTTAATGGGATACCAACCTGTGCAGATCCCGACCTTCTTGCTGGAGTTATCCGAGGCGAATGGAAACTAAATGGATACATTGTTACGGATTGTGATTCAATAGATGTGTACTACAACAACCAACACTACACCAAAACACCAGAGGAAGCTGCAGCTAAGGCTATATTGGCAGGGTTGGATCTCAACTGCGGATCTTTCCTGGGAAAATACACCCAAGGTGCAGTCCAAGCAGGACTCGTTAACGAGGCCGCCATTGACAGGGCCATCTCCAACAATTTTGCCACTTTGATGCGTCTAGGCTTCTTTGATGGTGATCCCAGCAACAAACCCTACGGAAAGCTTGGTCCAAAAGATGTGTGTACCTCAGAGAACCAGGAGCTAGCCCGTGAAACCGCGAGGCAAGGGATAGTACTGCTAAAAAACAGTCCAGGATCACTGCCTCTGTCTCCCACGGCCATCAAATCTTTGGCGGTCATTGGTCCCAATAGCAATGTCACAAAGACAATGATCGGAAACTACGAAGGCACGCCGTGCAAGTATACAACTATTCTGCAAGGTCTAAGTGCTTCAGCTGCAACAAGTTATGTTCCTGCCTGCGCCAATGTGGCTTGTGGTACTGCACAGGTAGATGATGCTACCAAGATAGCAGCCTCAGCAGATGCAACTATACTTGTCGTGGGTGCAGATCAATCGATTGAGGCAGAGAGTCGTGACCGGATTGACCTATATCTTCCGGGCCAGCAAACACTCCTAGTAACAGAAGTTGCAAAGGCATCCAAGGGCCCAGTAATCCTTGTAATAATGTCTGGAGGAGGATTCGACATTACATTCGCCAAAAACAATACTAAAATTACAAGCATACTTTGGGTTGGTTACCCCGGCGAAGCTGGTGGAGCCGCCGTAGCTGATGTAGTTTTTGGTCATTATAATCCATATATACATATCATTATCCTAATAGTGGCTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0031221 GO:0031222 GO:0043170 GO:0043207 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044464 GO:0045087 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:0098542 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

595

Amino Acids

63.56

Weight (kDa)

5.95

Isoelectric Point (pI)

31.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	120 - 361	1.3e-32	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	408 - 583	2.9e-39	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09730 AT5G64570 AT5G64570 AT5G64570
fragaria_vesca	FvH4_5g30690 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710
malus_domestica	MD08G1221800.v1.1 MD15G1414100.v1.1
prunus_persica	Prupe.1G559400_v2.0.a1 Prupe.1G559400_v2.0.a1
pyrus_communis	pycom08g19240 pycom15g36840
rosa_chinensis	RchiOBHm_Chr7g0225551 RchiOBHm_Chr7g0225581
rosa_laevigata	RLG00000001804 RLG00000001806
rosa_multiflora	Rmu_co8377913.1_g000001 Rmu_sc0003876.1_g000021 Rmu_sc0006321.1_g000006
rosa_roxburghii	Rroxscaffold_3G00233670 Rroxscaffold_3G00233690
rosa_rugosa	Rorug07G0231600 Rorug07G0231800
rosa_samantha	Rh7AG372300 Rh7AG372500 Rh7BG365400 Rh7BG365500 Rh7CG390600 Rh7CG390800 Rh7DG376200 Rh7DG376300
rosa_wichuraiana	Rw7G031750 Rw7G031760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1743
AasI	GACNNNNNNGTC	1 cut(s)	490
AccB1I	GGYRCC	1 cut(s)	332
AccI	GTMKAC	1 cut(s)	1355
AccII	CGCG	1 cut(s)	1211
AccIII	TCCGGA	1 cut(s)	584
AciI	CCGC	9 cut(s)	194, 208, 393, 755, 1005, 1065, 1209, 1286, 1713
AclWI	GGATC	7 cut(s)	576, 589, 836, 1002, 1015, 1124, 1255
AcoI	YGGCCR	1 cut(s)	1269
AcsI	RAATTY	2 cut(s)	76, 273
AcuI	CTGAAG	1 cut(s)	1368
AdeI	CACNNNGTG	2 cut(s)	313, 1347
AfaI	GTAC	5 cut(s)	461, 926, 1177, 1227, 1432
AfiI	CCNNNNNNNGG	5 cut(s)	429, 526, 527, 1074, 1774
AflII	CTTAAG	1 cut(s)	635
AgeI	ACCGGT	1 cut(s)	106
AgsI	TTSAA	8 cut(s)	53, 82, 403, 419, 605, 752, 763, 915
AjnI	CCWGG	6 cut(s)	328, 537, 813, 1014, 1188, 1243
Alw26I	GTCTC	4 cut(s)	353, 510, 536, 1266
AlwI	GGATC	7 cut(s)	576, 589, 836, 1002, 1015, 1124, 1255
Ama87I	CYCGRG	1 cut(s)	568
Aor13HI	TCCGGA	1 cut(s)	584
ApaI	GGGCCC	1 cut(s)	1601
ApeKI	GCWGC	6 cut(s)	644, 651, 965, 968, 1388, 1463
ApoI	RAATTY	2 cut(s)	76, 273
ArsI	GACNNNNNNTTYG	2 cut(s)	1628, 1660
AsiGI	ACCGGT	1 cut(s)	106
AspLEI	GCGC	1 cut(s)	1415
AspS9I	GGNCC	7 cut(s)	326, 1077, 1160, 1295, 1552, 1597, 1598
AsuC2I	CCSGG	4 cut(s)	357, 549, 1551, 1695
AsuHPI	GGTGA	8 cut(s)	96, 214, 253, 486, 563, 632, 733, 1139
AsuNHI	GCTAGC	2 cut(s)	128, 1195
AvaI	CYCGRG	1 cut(s)	568
AvaII	GGWCC	2 cut(s)	1160, 1295
AxyI	CCTNAGG	1 cut(s)	375
BaeGI	GKGCMC	1 cut(s)	1601
BamHI	GGATCC	1 cut(s)	581
BanI	GGYRCC	1 cut(s)	332
BanII	GRGCYC	1 cut(s)	1601
BbsI	GAAGAC	1 cut(s)	561
BbvCI	CCTCAGC	1 cut(s)	1468
BbvI	GCAGC	6 cut(s)	631, 638, 952, 980, 1375, 1475
BccI	CCATC	6 cut(s)	519, 611, 773, 1088, 1118, 1280
BceAI	ACGGC	5 cut(s)	325, 742, 1284, 1330, 1700
BcgI	CGANNNNNNTGC	4 cut(s)	836, 870, 1488, 1522
BciT130I	CCWGG	6 cut(s)	330, 539, 815, 1016, 1190, 1245
BciVI	GTATCC	2 cut(s)	819, 884
BcnI	CCSGG	4 cut(s)	357, 549, 1551, 1695
BcoDI	GTCTC	4 cut(s)	353, 510, 536, 1266
BfaI	CTAG	5 cut(s)	129, 1112, 1196, 1568, 1779
BfmI	CTRYAG	1 cut(s)	966
BfrI	CTTAAG	1 cut(s)	635
BfuI	GTATCC	2 cut(s)	819, 884
BglI	GCCNNNNNGGC	1 cut(s)	1421
BmcAI	AGTACT	1 cut(s)	1227
Bme18I	GGWCC	2 cut(s)	1160, 1295
BmeT110I	CYCGRG	1 cut(s)	568
BmgT120I	GGNCC	7 cut(s)	326, 1077, 1160, 1295, 1552, 1597, 1598
BmiI	GGNNCC	5 cut(s)	334, 583, 1297, 1599, 1711
BmrI	ACTGGG	1 cut(s)	1595
BmsI	GCATC	5 cut(s)	172, 1095, 1438, 1465, 1598
BmtI	GCTAGC	2 cut(s)	132, 1199
BmuI	ACTGGG	1 cut(s)	1595
BpiI	GAAGAC	1 cut(s)	561
BpmI	CTGGAG	2 cut(s)	879, 1644
Bpu10I	CCTNAGC	2 cut(s)	972, 1468
BpuMI	CCSGG	4 cut(s)	357, 549, 1551, 1695
Bsa29I	ATCGAT	2 cut(s)	777, 1508
BsaBI	GATNNNNATC	1 cut(s)	1128
BsaI	GGTCTC	1 cut(s)	510
BsaJI	CCNNGG	7 cut(s)	328, 868, 1015, 1030, 1266, 1593, 1693
BsaWI	WCCGGW	3 cut(s)	106, 584, 1530
BsaXI	ACNNNNNCTCC	2 cut(s)	1701, 1731
Bsc4I	CCNNNNNNNGG	5 cut(s)	429, 526, 527, 1074, 1774
Bse118I	RCCGGY	1 cut(s)	106
Bse1I	ACTGG	1 cut(s)	1601
Bse21I	CCTNAGG	1 cut(s)	375
Bse3DI	GCAATG	4 cut(s)	127, 462, 790, 1312
Bse8I	GATNNNNATC	1 cut(s)	1128
BseAI	TCCGGA	1 cut(s)	584
BseBI	CCWGG	6 cut(s)	330, 539, 815, 1016, 1190, 1245
BseCI	ATCGAT	2 cut(s)	777, 1508
BseDI	CCNNGG	7 cut(s)	328, 868, 1015, 1030, 1266, 1593, 1693
BseGI	GGATG	2 cut(s)	381, 1589
BseJI	GATNNNNATC	1 cut(s)	1128
BseLI	CCNNNNNNNGG	5 cut(s)	429, 526, 527, 1074, 1774
BseMI	GCAATG	4 cut(s)	127, 462, 790, 1312
BseMII	CTCAG	3 cut(s)	389, 1194, 1482
BseNI	ACTGG	1 cut(s)	1601
BseRI	GAGGAG	1 cut(s)	1641
BseSI	GKGCMC	1 cut(s)	1601
BseXI	GCAGC	6 cut(s)	631, 638, 952, 980, 1375, 1475
BseYI	CCCAGC	2 cut(s)	172, 1133
BsgI	GTGCAG	5 cut(s)	270, 858, 1056, 1419, 1518
Bsh1236I	CGCG	1 cut(s)	1211
BshNI	GGYRCC	1 cut(s)	332
BshTI	ACCGGT	1 cut(s)	106
BshVI	ATCGAT	2 cut(s)	777, 1508
BsiHKCI	CYCGRG	1 cut(s)	568
BsiSI	CCGG	7 cut(s)	107, 357, 548, 585, 1531, 1550, 1695
BslFI	GGGAC	1 cut(s)	1281
BslI	CCNNNNNNNGG	5 cut(s)	429, 526, 527, 1074, 1774
BsmAI	GTCTC	4 cut(s)	353, 510, 536, 1266
BsmFI	GGGAC	1 cut(s)	1281
Bso31I	GGTCTC	1 cut(s)	510
BsoBI	CYCGRG	1 cut(s)	568
Bsp120I	GGGCCC	1 cut(s)	1597
Bsp1286I	GDGCHC	1 cut(s)	1601
Bsp13I	TCCGGA	1 cut(s)	584
Bsp1407I	TGTACA	1 cut(s)	459
Bsp143I	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
BspACI	CCGC	9 cut(s)	194, 208, 393, 755, 1005, 1065, 1209, 1286, 1713
BspCNI	CTCAG	3 cut(s)	388, 1193, 1481
BspDI	ATCGAT	2 cut(s)	777, 1508
BspEI	TCCGGA	1 cut(s)	584
BspFNI	CGCG	1 cut(s)	1211
BspLI	GGNNCC	5 cut(s)	334, 583, 1297, 1599, 1711
BspMAI	CTGCAG	1 cut(s)	970
BspOI	GCTAGC	2 cut(s)	132, 1199
BspPI	GGATC	7 cut(s)	576, 589, 836, 1002, 1015, 1124, 1255
BspT107I	GGYRCC	1 cut(s)	332
BspTI	CTTAAG	1 cut(s)	635
BspTNI	GGTCTC	1 cut(s)	510
BsrDI	GCAATG	4 cut(s)	127, 462, 790, 1312
BsrFI	RCCGGY	1 cut(s)	106
BsrGI	TGTACA	1 cut(s)	459
BsrI	ACTGG	1 cut(s)	1601
BssAI	RCCGGY	1 cut(s)	106
BssECI	CCNNGG	7 cut(s)	328, 868, 1015, 1030, 1266, 1593, 1693
BssMI	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
BssNAI	GTATAC	1 cut(s)	1356
BssT1I	CCWWGG	2 cut(s)	1030, 1593
Bst1107I	GTATAC	1 cut(s)	1356
Bst2UI	CCWGG	6 cut(s)	330, 539, 815, 1016, 1190, 1245
Bst4CI	ACNGT	2 cut(s)	248, 1241
Bst6I	CTCTTC	1 cut(s)	526
BstAFI	CTTAAG	1 cut(s)	635
BstAPI	GCANNNNNTGC	1 cut(s)	1347
BstAUI	TGTACA	1 cut(s)	459
BstC8I	GCNNGC	8 cut(s)	118, 130, 266, 649, 1197, 1343, 1411, 1556
BstDEI	CTNAG	5 cut(s)	375, 972, 1180, 1376, 1468
BstDSI	CCRYGG	1 cut(s)	1266
BstEII	GGTNACC	2 cut(s)	492, 1688
BstF5I	GGATG	2 cut(s)	381, 1589
BstFNI	CGCG	1 cut(s)	1211
BstHHI	GCGC	1 cut(s)	1415
BstKTI	GATC	8 cut(s)	584, 844, 997, 1010, 1132, 1250, 1326, 1504
BstMAI	GTCTC	4 cut(s)	353, 510, 536, 1266
BstMBI	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
BstNI	CCWGG	6 cut(s)	330, 539, 815, 1016, 1190, 1245
BstNSI	RCATGY	1 cut(s)	651
BstPI	GGTNACC	2 cut(s)	492, 1688
BstSFI	CTRYAG	1 cut(s)	966
BstSLI	GKGCMC	1 cut(s)	1601
BstUI	CGCG	1 cut(s)	1211
BstV1I	GCAGC	6 cut(s)	631, 638, 952, 980, 1375, 1475
BstV2I	GAAGAC	1 cut(s)	561
BstX2I	RGATCY	4 cut(s)	581, 841, 994, 1007
BstXI	CCANNNNNNTGG	1 cut(s)	821
BstYI	RGATCY	4 cut(s)	581, 841, 994, 1007
BstZ17I	GTATAC	1 cut(s)	1356
Bsu15I	ATCGAT	2 cut(s)	777, 1508
Bsu36I	CCTNAGG	1 cut(s)	375
BsuI	GTATCC	2 cut(s)	819, 884
BsuTUI	ATCGAT	2 cut(s)	777, 1508
BtgI	CCRYGG	1 cut(s)	1266
BtgZI	GCGATG	1 cut(s)	153
BtsCI	GGATG	2 cut(s)	381, 1589
BtsI	GCAGTG	3 cut(s)	387, 723, 1250
BtsIMutI	CAGTG	4 cut(s)	253, 387, 723, 1250
Cac8I	GCNNGC	8 cut(s)	118, 130, 266, 649, 1197, 1343, 1411, 1556
CfoI	GCGC	1 cut(s)	1415
Cfr10I	RCCGGY	1 cut(s)	106
Cfr13I	GGNCC	7 cut(s)	326, 1077, 1160, 1295, 1552, 1597, 1598
ClaI	ATCGAT	2 cut(s)	777, 1508
CseI	GACGC	1 cut(s)	1097
CsiI	ACCWGGT	1 cut(s)	813
Csp6I	GTAC	5 cut(s)	460, 925, 1176, 1226, 1431
CspAI	ACCGGT	1 cut(s)	106
CviAII	CATG	2 cut(s)	648, 799
CviQI	GTAC	5 cut(s)	460, 925, 1176, 1226, 1431
DdeI	CTNAG	5 cut(s)	375, 972, 1180, 1376, 1468
DpnI	GATC	8 cut(s)	583, 843, 996, 1009, 1131, 1249, 1325, 1503
DpnII	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
DraIII	CACNNNGTG	2 cut(s)	313, 1347
DrdI	GACNNNNNNGTC	1 cut(s)	490
DseDI	GACNNNNNNGTC	1 cut(s)	490
EaeI	YGGCCR	1 cut(s)	1269
Eam1104I	CTCTTC	1 cut(s)	526
EarI	CTCTTC	1 cut(s)	526
EciI	GGCGGA	1 cut(s)	209
Eco130I	CCWWGG	2 cut(s)	1030, 1593
Eco147I	AGGCCT	1 cut(s)	600
Eco24I	GRGCYC	1 cut(s)	1601
Eco31I	GGTCTC	1 cut(s)	510
Eco47I	GGWCC	2 cut(s)	1160, 1295
Eco57I	CTGAAG	1 cut(s)	1368
Eco81I	CCTNAGG	1 cut(s)	375
Eco88I	CYCGRG	1 cut(s)	568
Eco91I	GGTNACC	2 cut(s)	492, 1688
EcoO109I	RGGNCCY	1 cut(s)	1597
EcoO65I	GGTNACC	2 cut(s)	492, 1688
EcoRI	GAATTC	1 cut(s)	273
EcoRII	CCWGG	6 cut(s)	328, 537, 813, 1014, 1188, 1243
EcoT14I	CCWWGG	2 cut(s)	1030, 1593
EcoT38I	GRGCYC	1 cut(s)	1601
ErhI	CCWWGG	2 cut(s)	1030, 1593
FaeI	CATG	2 cut(s)	651, 802
FaqI	GGGAC	1 cut(s)	1281
FatI	CATG	2 cut(s)	647, 798
FblI	GTMKAC	1 cut(s)	1355
FokI	GGATG	2 cut(s)	368, 1576
FriOI	GRGCYC	1 cut(s)	1601
FspBI	CTAG	5 cut(s)	129, 1112, 1196, 1568, 1779
GlaI	GCGC	1 cut(s)	1414
GsaI	CCCAGC	2 cut(s)	176, 1137
GsuI	CTGGAG	2 cut(s)	879, 1644
HapII	CCGG	7 cut(s)	107, 357, 548, 585, 1531, 1550, 1695
HgaI	GACGC	1 cut(s)	1097
HhaI	GCGC	1 cut(s)	1415
Hin1II	CATG	2 cut(s)	651, 802
Hin6I	GCGC	1 cut(s)	1413
HinP1I	GCGC	1 cut(s)	1413
HincII	GTYRAC	2 cut(s)	697, 1057
HindII	GTYRAC	2 cut(s)	697, 1057
HindIII	AAGCTT	2 cut(s)	632, 1154
HinfI	GANTC	6 cut(s)	346, 544, 911, 1050, 1522, 1632
HpaI	GTTAAC	1 cut(s)	1057
HpaII	CCGG	7 cut(s)	107, 357, 548, 585, 1531, 1550, 1695
HphI	GGTGA	8 cut(s)	96, 214, 253, 486, 563, 632, 733, 1139
Hpy166II	GTNNAC	6 cut(s)	244, 697, 925, 1057, 1176, 1356
Hpy188I	TCNGA	4 cut(s)	298, 869, 1183, 1328
Hpy188III	TCNNGA	6 cut(s)	515, 568, 585, 845, 1526, 1623
Hpy8I	GTNNAC	6 cut(s)	244, 697, 925, 1057, 1176, 1356
Hpy99I	CGWCG	3 cut(s)	326, 445, 617
HpyAV	CCTTC	5 cut(s)	76, 166, 611, 860, 1331
HpyCH4III	ACNGT	2 cut(s)	248, 1241
HpyCH4IV	ACGT	1 cut(s)	321
HpyF3I	CTNAG	5 cut(s)	375, 972, 1180, 1376, 1468
HpySE526I	ACGT	1 cut(s)	321
Hsp92II	CATG	2 cut(s)	651, 802
HspAI	GCGC	1 cut(s)	1413
Kpn2I	TCCGGA	1 cut(s)	584
KspAI	GTTAAC	1 cut(s)	1057
Kzo9I	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
LmnI	GCTCC	4 cut(s)	44, 359, 1192, 1709
Lsp1109I	GCAGC	6 cut(s)	631, 638, 952, 980, 1375, 1475
LweI	GCATC	5 cut(s)	172, 1095, 1438, 1465, 1598
MabI	ACCWGGT	1 cut(s)	813
MaeI	CTAG	5 cut(s)	129, 1112, 1196, 1568, 1779
MaeII	ACGT	1 cut(s)	321
MaeIII	GTNAC	8 cut(s)	102, 492, 721, 898, 1309, 1526, 1570, 1688
MalI	GATC	8 cut(s)	583, 843, 996, 1009, 1131, 1249, 1325, 1503
MboI	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
MboII	GAAGA	3 cut(s)	543, 566, 1538
MfeI	CAATTG	1 cut(s)	682
MflI	RGATCY	4 cut(s)	581, 841, 994, 1007
MhlI	GDGCHC	1 cut(s)	1601
MluCI	AATT	5 cut(s)	76, 273, 682, 1090, 1665
MlyI	GAGTC	4 cut(s)	355, 538, 1044, 1531
MmeI	TCCRAC	3 cut(s)	276, 972, 1110
MroI	TCCGGA	1 cut(s)	584
MseI	TTAA	5 cut(s)	504, 636, 711, 819, 1056
MslI	CAYNNNNRTG	2 cut(s)	711, 1103
MspA1I	CMGCKG	1 cut(s)	1388
MspCI	CTTAAG	1 cut(s)	635
MspI	CCGG	7 cut(s)	107, 357, 548, 585, 1531, 1550, 1695
MunI	CAATTG	1 cut(s)	682
MvaI	CCWGG	6 cut(s)	330, 539, 815, 1016, 1190, 1245
MvnI	CGCG	1 cut(s)	1211
NciI	CCSGG	4 cut(s)	357, 549, 1551, 1695
NdeII	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
NheI	GCTAGC	2 cut(s)	128, 1195
NlaIII	CATG	2 cut(s)	651, 802
NlaIV	GGNNCC	5 cut(s)	334, 583, 1297, 1599, 1711
NmuCI	GTSAC	5 cut(s)	102, 492, 721, 1309, 1526
NspI	RCATGY	1 cut(s)	651
PaeI	GCATGC	1 cut(s)	651
PaeR7I	CTCGAG	1 cut(s)	568
PceI	AGGCCT	1 cut(s)	600
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 1332
PfeI	GAWTC	2 cut(s)	911, 1632
PfoI	TCCNGGA	2 cut(s)	355, 1243
PinAI	ACCGGT	1 cut(s)	106
PleI	GAGTC	4 cut(s)	354, 538, 1044, 1530
PpsI	GAGTC	4 cut(s)	354, 538, 1044, 1530
PsiI	TTATAA	1 cut(s)	1743
Psp6I	CCWGG	6 cut(s)	328, 537, 813, 1014, 1188, 1243
PspEI	GGTNACC	2 cut(s)	492, 1688
PspFI	CCCAGC	2 cut(s)	172, 1133
PspGI	CCWGG	6 cut(s)	328, 537, 813, 1014, 1188, 1243
PspN4I	GGNNCC	5 cut(s)	334, 583, 1297, 1599, 1711
PspOMI	GGGCCC	1 cut(s)	1597
PspPI	GGNCC	7 cut(s)	326, 1077, 1160, 1295, 1552, 1597, 1598
PstI	CTGCAG	1 cut(s)	970
PsuI	RGATCY	4 cut(s)	581, 841, 994, 1007
PvuII	CAGCTG	1 cut(s)	1388
RsaI	GTAC	5 cut(s)	461, 926, 1177, 1227, 1432
RsaNI	GTAC	5 cut(s)	460, 925, 1176, 1226, 1431
RseI	CAYNNNNRTG	2 cut(s)	711, 1103
SaqAI	TTAA	5 cut(s)	504, 636, 711, 819, 1056
Sau3AI	GATC	8 cut(s)	581, 841, 994, 1007, 1129, 1247, 1323, 1501
Sau96I	GGNCC	7 cut(s)	326, 1077, 1160, 1295, 1552, 1597, 1598
ScaI	AGTACT	1 cut(s)	1227
SchI	GAGTC	4 cut(s)	355, 538, 1044, 1531
SduI	GDGCHC	1 cut(s)	1601
SexAI	ACCWGGT	1 cut(s)	813
SfaNI	GCATC	5 cut(s)	172, 1095, 1438, 1465, 1598
SfcI	CTRYAG	1 cut(s)	966
Sfr274I	CTCGAG	1 cut(s)	568
SinI	GGWCC	2 cut(s)	1160, 1295
SlaI	CTCGAG	1 cut(s)	568
SmiMI	CAYNNNNRTG	2 cut(s)	711, 1103
SmlI	CTYRAG	2 cut(s)	568, 635
SmoI	CTYRAG	2 cut(s)	568, 635
SphI	GCATGC	1 cut(s)	651
Sse9I	AATT	5 cut(s)	76, 273, 682, 1090, 1665
SseBI	AGGCCT	1 cut(s)	600
SsiI	CCGC	9 cut(s)	194, 208, 393, 755, 1005, 1065, 1209, 1286, 1713
SspI	AATATT	1 cut(s)	502
SspMI	CTAG	5 cut(s)	129, 1112, 1196, 1568, 1779
StuI	AGGCCT	1 cut(s)	600
StyI	CCWWGG	2 cut(s)	1030, 1593
TaaI	ACNGT	2 cut(s)	248, 1241
TaiI	ACGT	1 cut(s)	324
TasI	AATT	5 cut(s)	76, 273, 682, 1090, 1665
TatI	WGTACW	3 cut(s)	459, 924, 1225
TauI	GCSGC	4 cut(s)	211, 395, 1067, 1715
TfiI	GAWTC	2 cut(s)	911, 1632
Tru1I	TTAA	5 cut(s)	504, 636, 711, 819, 1056
Tru9I	TTAA	5 cut(s)	504, 636, 711, 819, 1056
TscAI	CASTG	4 cut(s)	253, 394, 723, 1257
TseFI	GTSAC	5 cut(s)	102, 492, 721, 1309, 1526
TseI	GCWGC	6 cut(s)	644, 651, 965, 968, 1388, 1463
Tsp45I	GTSAC	5 cut(s)	102, 492, 721, 1309, 1526
TspGWI	ACGGA	4 cut(s)	458, 594, 917, 1164
TspRI	CASTG	4 cut(s)	253, 394, 723, 1257
Vha464I	CTTAAG	1 cut(s)	635
VpaK11BI	GGWCC	2 cut(s)	1160, 1295
XapI	RAATTY	2 cut(s)	76, 273
XceI	RCATGY	1 cut(s)	651
XcmI	CCANNNNNNNNNTGG	2 cut(s)	430, 1279
XhoI	CTCGAG	1 cut(s)	568
XmiI	GTMKAC	1 cut(s)	1355
XspI	CTAG	5 cut(s)	129, 1112, 1196, 1568, 1779
ZrmI	AGTACT	1 cut(s)	1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.