Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003333

Physical Location & Seq

Genomic Coordinates Reverse (-)

48427249 .. 48431493

Gene Structure 4245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ20204

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGCCTCCTTTTTTTTCTTTTCCTCTGTTCAGAACAGAGTACTTCAAAACAGAGCAGTGCCGAAGGCCTTGCTCTGTTTTACTCTCTGTTACATACTGATGTTATCTTTCTGTATGAGCTCAGTTTCCGGCCAGTCATTTGCCTGCGGTGGAGGTTCCAGCAGCTTCCCGTTCTGCGACAAGTCGTTGGCGGCCGATGCGAGGGTGGCGGACCTCGTGAAGAGGCTGACATTGCAGGAGAAGATTACGTTTCTGGTGAACAGTGCAGGCAGTGTGAGCAGGCTTGGAATTCCCAAGTACGAGTGGTGGTCGGAGGCTTTACACGGTGTGTCGTACGTCGGCCCCGGGACCAAGTTTTCGAATGTCGTCCCCGGAGCTACCAGCTTCCCTCAGCCCATTTTGACCGCAGCGTCGTTCAACAACTCTCTCTACGAAGCCATCGGAAGGGTGGTGTCAACTGAGGCCAGAGCAATGTACAATGTGGGATTGGCAGGATTGACATTCTGGTCCCCAAACATTAACATTTTCCGAGACCCGAGATGGGGAAGAGGCCAAGAGACTCCGGGAGAAGACCCATTGCTCACGAGTAACTACGGATCAGGATACGTTAGAGGTCTTCAACAAACCGATAGCGGAGACAAAAATAGGCTTAAAGTTGCAGCATGTTGTAAACATTATACAGCCTATGATTTGGACAATTGGAAAGGAACTGACAGATACCATTTCAATGCAGTGGTAACAAAACAAGACATGGATGATACATTTCAGCCACCATTCAAAAGTTGTGTTCTTGATGGGAATGTTGCTAGTGTCATGTGTTCTTACAACCAGGTTAATGGAATACCTACCTGCGCAGACCCAAACCTTCTTTCTGGGGTTATCCGAGGCGAATGGAAGTTGAATGGATACATAGTGACTGATTGTGATTCAATAGATGTGTACTACAAGAACCAACACTACACCAAGACACCAGAAGAGGCTGCAGCTAAGGCTATATTAGCAGGTTTGGATCTAAACTGTGGATCATTCTTAGGACAGTACACAGAAGGAGCAGTGAAAGGAGGACTTGTGACTGAGGCAGCCATTGACAAGGCCATCACAAACAATTTTGGCACATTGATGCGTCTAGGCTTCTTCGATGGTGATCCAAGCAAGCAACCATATGGACATCTTGGTCCAAAGGATGTGTGCACTCCACAGAACCAGGAGCTGGCACGTGAAACAGCGAGACAAGGGATAGTGTTGCTCAAGAACACTCCAGGATCACTGCCTCTGTCTCCCACAGCCATCAAATCCTTGGCAGTTATAGGTCCCAATGGCAATGTCACAAAGACTATGATTGGAAACTACGAAGGCACCCCTTGCAAATATACAACTCCTTTGCAAGGCCTAACAGCCGTAGTTCCCACAACATTTGTGGCCGGGTGCGCTGATGTGGCTTGTGGCACTGCCCAGGTAGATGATGCTACCAAGTTAGCAGCCGCAGCAGATGCAACTGTACTTATAGTGGGTGAAAATCAATCAATTGAGACTGAAAGTCATGATAGAATCGACCTCCATCTTCCAGGACAGCAAACTCTCCTAATAACTGAAGTAGCGAAGGCTTCTAAAGGACCTGTTATCCTTGTCATAATGTCTGGAGGAGGGTTCGATATTACCTTTGCGAAAAATGATGCTAAAATTACAAGCATTCTATGGGTTGGTTACCCTGGCGAAGCTGGTGGAGCTGCCATTGCTGACGTGATTTTTGGCTTTTATAATCCAAGTGGAAGACTACCTATGACGTGGTATCCTCAAGCATATGTAGACAAGGTCCCTATGACAAACATGAATATGAGACCAGATCCTGCGAGTGGTTACCCGGGCAGGACTTACCGGTTTTACACTGGTGACACGGTTTATGAATTTGGATACGGAATAAGCTACTCCACCTTCAATCACCGCCTAGTTCGAGCACCGAAGCAAGTTTCCATTCCCTTAGAGGAGGATCATGTATGCCACTCATCAAGTTGCCAATCACTTGATGTTGTAGAAGAAAGGTGTCAAAATCTAGATTTCGATATCCATTTGGGGGTGAAAAACATGGGCAAAATGAGTGGAAGTCATACAGTCTTATTGTATTCGAGTCCTCCATCTGTGCACAATTCTCCTAAAAAGCACTTGTTGGGATTTGAGAAGGTGTTCCTTAGCTCTCAGGGAGAAGCATTGGTCAAGTTCAATGTGGATGTTTGCAAGCACTTGAGTGTGGTTGATGAGCTAGGAAGTAGGAAAGTTGCTTTGGGAGATCATGTACTACATGTGGGAGACTTGAAACAGACATTGAGTGTGAGGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009627 GO:0009814 GO:0009987 GO:0010383 GO:0010410 GO:0016052 GO:0016787 GO:0016798 GO:0030312 GO:0031221 GO:0031222 GO:0043170 GO:0043207 GO:0044036 GO:0044237 GO:0044238 GO:0044260 GO:0044464 GO:0045087 GO:0045491 GO:0045493 GO:0046556 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0071554 GO:0071704 GO:0071944 GO:0097599 GO:0098542 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

778

Amino Acids

84.22

Weight (kDa)

6.5

Isoelectric Point (pI)

33.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	123 - 366	8.4e-34	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	413 - 642	1e-50	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	702 - 769	7.5e-10	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09730 AT5G64570 AT5G64570 AT5G64570
fragaria_vesca	FvH4_5g30690 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710 FvH4_5g30710
malus_domestica	MD08G1221800.v1.1 MD15G1414100.v1.1
prunus_persica	Prupe.1G559400_v2.0.a1 Prupe.1G559400_v2.0.a1
pyrus_communis	pycom08g19240 pycom15g36840
rosa_chinensis	RchiOBHm_Chr7g0225551 RchiOBHm_Chr7g0225581
rosa_laevigata	RLG00000001804 RLG00000001806
rosa_multiflora	Rmu_co8377913.1_g000001 Rmu_sc0003876.1_g000021 Rmu_sc0006321.1_g000006
rosa_roxburghii	Rroxscaffold_3G00233670 Rroxscaffold_3G00233690
rosa_rugosa	Rorug07G0231600 Rorug07G0231800
rosa_samantha	Rh7AG372300 Rh7AG372500 Rh7BG365400 Rh7BG365500 Rh7CG390600 Rh7CG390800 Rh7DG376200 Rh7DG376300
rosa_wichuraiana	Rw7G031750 Rw7G031760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1758
AasI	GACNNNNNNGTC	3 cut(s)	409, 505, 1173
Acc16I	TGCGCA	1 cut(s)	853
Acc36I	ACCTGC	2 cut(s)	857, 992
AccB1I	GGYRCC	1 cut(s)	1355
AccB7I	CCANNNNNTGG	1 cut(s)	1210
AccI	GTMKAC	1 cut(s)	1806
AciI	CCGC	7 cut(s)	147, 191, 209, 405, 633, 1482, 1942
AclWI	GGATC	7 cut(s)	604, 1017, 1030, 1139, 1270, 1838, 1995
AcoI	YGGCCR	3 cut(s)	130, 192, 1419
AcsI	RAATTY	2 cut(s)	288, 1904
AcuI	CTGAAG	1 cut(s)	1611
AcvI	CACGTG	1 cut(s)	1217
AdeI	CACNNNGTG	1 cut(s)	328
AfaI	GTAC	8 cut(s)	42, 299, 335, 476, 941, 1040, 1500, 2292
AfiI	CCNNNNNNNGG	8 cut(s)	201, 444, 541, 542, 1210, 1385, 1853, 2071
AflIII	ACRYGT	1 cut(s)	2296
AgeI	ACCGGT	1 cut(s)	1875
AhdI	GACNNNNNGTC	1 cut(s)	1536
AjiI	CACGTC	2 cut(s)	1741, 1785
AjnI	CCWGG	6 cut(s)	828, 1203, 1258, 1452, 1564, 1708
AjuI	GAANNNNNNNTTGG	4 cut(s)	853, 885, 2261, 2293
AleI	CACNNNNGTG	1 cut(s)	2241
Alw21I	GWGCWC	4 cut(s)	122, 1193, 1957, 2142
Alw26I	GTCTC	8 cut(s)	525, 551, 630, 1222, 1281, 1523, 1831, 2298
Alw44I	GTGCAC	2 cut(s)	1189, 2138
AlwI	GGATC	7 cut(s)	604, 1017, 1030, 1139, 1270, 1838, 1995
AlwNI	CAGNNNCTG	2 cut(s)	1210, 1847
Ama87I	CYCGRG	3 cut(s)	344, 535, 1861
ApaLI	GTGCAC	2 cut(s)	1189, 2138
ApeKI	GCWGC	9 cut(s)	162, 407, 659, 980, 983, 1079, 1478, 1484, 1727
ApoI	RAATTY	2 cut(s)	288, 1904
ArsI	GACNNNNNNTTYG	4 cut(s)	340, 372, 1730, 1762
AsiGI	ACCGGT	1 cut(s)	1875
Asp700I	GAANNNNTTC	1 cut(s)	2180
AspLEI	GCGC	2 cut(s)	854, 1430
AspS9I	GGNCC	8 cut(s)	211, 341, 348, 507, 1175, 1310, 1613, 1813
AsuC2I	CCSGG	7 cut(s)	345, 346, 372, 564, 1423, 1862, 1863
AsuHPI	GGTGA	6 cut(s)	268, 1154, 1523, 1901, 1931, 2086
AsuII	TTCGAA	1 cut(s)	359
AvaI	CYCGRG	3 cut(s)	344, 535, 1861
AvaII	GGWCC	7 cut(s)	211, 348, 507, 1175, 1310, 1613, 1813
BaeGI	GKGCMC	2 cut(s)	1193, 2142
BanI	GGYRCC	1 cut(s)	1355
BanII	GRGCYC	1 cut(s)	122
BauI	CACGAG	2 cut(s)	215, 583
BbrPI	CACGTG	1 cut(s)	1217
BbsI	GAAGAC	3 cut(s)	576, 608, 1777
Bbv12I	GWGCWC	4 cut(s)	122, 1193, 1957, 2142
BbvCI	CCTCAGC	1 cut(s)	390
BbvI	GCAGC	9 cut(s)	174, 419, 671, 967, 995, 1091, 1490, 1496, 1714
BccI	CCATC	8 cut(s)	446, 534, 788, 1103, 1133, 1295, 1566, 2140
BceAI	ACGGC	1 cut(s)	1382
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	6 cut(s)	830, 1205, 1260, 1454, 1566, 1710
BciVI	GTATCC	4 cut(s)	596, 899, 1800, 1904
BcnI	CCSGG	7 cut(s)	345, 346, 372, 564, 1423, 1862, 1863
BcoDI	GTCTC	8 cut(s)	525, 551, 630, 1222, 1281, 1523, 1831, 2298
BfaI	CTAG	5 cut(s)	807, 1127, 1946, 2051, 2258
BfmI	CTRYAG	1 cut(s)	981
BfuAI	ACCTGC	2 cut(s)	857, 992
BfuI	GTATCC	4 cut(s)	596, 899, 1800, 1904
BmcAI	AGTACT	1 cut(s)	42
Bme18I	GGWCC	7 cut(s)	211, 348, 507, 1175, 1310, 1613, 1813
BmeRI	GACNNNNNGTC	1 cut(s)	1536
BmeT110I	CYCGRG	3 cut(s)	344, 535, 1861
BmgBI	CACGTC	2 cut(s)	1741, 1785
BmgT120I	GGNCC	8 cut(s)	211, 341, 348, 507, 1175, 1310, 1613, 1813
BmiI	GGNNCC	7 cut(s)	157, 343, 349, 509, 1312, 1357, 1815
BmsI	GCATC	5 cut(s)	187, 1110, 1453, 1480, 1663
BpiI	GAAGAC	3 cut(s)	576, 608, 1777
BpmI	CTGGAG	2 cut(s)	1242, 1659
Bpu10I	CCTNAGC	3 cut(s)	390, 987, 2186
Bpu14I	TTCGAA	1 cut(s)	359
BpuEI	CTTGAG	3 cut(s)	1232, 1779, 2260
BpuMI	CCSGG	7 cut(s)	345, 346, 372, 564, 1423, 1862, 1863
BsaAI	YACGTR	1 cut(s)	1217
BsaBI	GATNNNNATC	1 cut(s)	1143
BsaI	GGTCTC	2 cut(s)	525, 1831
BsaJI	CCNNGG	8 cut(s)	343, 344, 370, 883, 1296, 1452, 1708, 1861
BsaWI	WCCGGW	1 cut(s)	1875
BsaXI	ACNNNNNCTCC	4 cut(s)	1053, 1083, 2274, 2304
Bsc4I	CCNNNNNNNGG	8 cut(s)	201, 444, 541, 542, 1210, 1385, 1853, 2071
Bse118I	RCCGGY	1 cut(s)	1875
Bse1I	ACTGG	2 cut(s)	133, 1891
Bse3DI	GCAATG	5 cut(s)	230, 477, 575, 1327, 1731
Bse8I	GATNNNNATC	1 cut(s)	1143
BseBI	CCWGG	6 cut(s)	830, 1205, 1260, 1454, 1566, 1710
BseDI	CCNNGG	8 cut(s)	343, 344, 370, 883, 1296, 1452, 1708, 1861
BseGI	GGATG	3 cut(s)	760, 1189, 2230
BseJI	GATNNNNATC	1 cut(s)	1143
BseLI	CCNNNNNNNGG	8 cut(s)	201, 444, 541, 542, 1210, 1385, 1853, 2071
BseMI	GCAATG	5 cut(s)	230, 477, 575, 1327, 1731
BseMII	CTCAG	5 cut(s)	135, 404, 450, 1065, 2207
BseNI	ACTGG	2 cut(s)	133, 1891
BseRI	GAGGAG	2 cut(s)	1656, 1997
BseSI	GKGCMC	2 cut(s)	1193, 2142
BseX3I	CGGCCG	1 cut(s)	192
BseXI	GCAGC	9 cut(s)	174, 419, 671, 967, 995, 1091, 1490, 1496, 1714
BsgI	GTGCAG	1 cut(s)	285
Bsh1285I	CGRYCG	1 cut(s)	195
BshNI	GGYRCC	1 cut(s)	1355
BshTI	ACCGGT	1 cut(s)	1875
BsiEI	CGRYCG	1 cut(s)	195
BsiHKAI	GWGCWC	4 cut(s)	122, 1193, 1957, 2142
BsiHKCI	CYCGRG	3 cut(s)	344, 535, 1861
BsiSI	CCGG	7 cut(s)	129, 345, 372, 563, 1422, 1862, 1876
BsiWI	CGTACG	1 cut(s)	333
BslFI	GGGAC	5 cut(s)	353, 361, 493, 1296, 1799
BslI	CCNNNNNNNGG	8 cut(s)	201, 444, 541, 542, 1210, 1385, 1853, 2071
BsmAI	GTCTC	8 cut(s)	525, 551, 630, 1222, 1281, 1523, 1831, 2298
BsmFI	GGGAC	5 cut(s)	353, 361, 493, 1296, 1799
BsmI	GAATGC	1 cut(s)	1689
Bso31I	GGTCTC	2 cut(s)	525, 1831
BsoBI	CYCGRG	3 cut(s)	344, 535, 1861
Bsp119I	TTCGAA	1 cut(s)	359
Bsp1286I	GDGCHC	4 cut(s)	122, 1193, 1957, 2142
Bsp1407I	TGTACA	1 cut(s)	474
Bsp143I	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
BspACI	CCGC	7 cut(s)	147, 191, 209, 405, 633, 1482, 1942
BspCNI	CTCAG	5 cut(s)	134, 403, 451, 1066, 2206
BspHI	TCATGA	1 cut(s)	1540
BspLI	GGNNCC	7 cut(s)	157, 343, 349, 509, 1312, 1357, 1815
BspMAI	CTGCAG	1 cut(s)	985
BspMI	ACCTGC	2 cut(s)	857, 992
BspPI	GGATC	7 cut(s)	604, 1017, 1030, 1139, 1270, 1838, 1995
BspT104I	TTCGAA	1 cut(s)	359
BspT107I	GGYRCC	1 cut(s)	1355
BspTNI	GGTCTC	2 cut(s)	525, 1831
BsrDI	GCAATG	5 cut(s)	230, 477, 575, 1327, 1731
BsrFI	RCCGGY	1 cut(s)	1875
BsrGI	TGTACA	1 cut(s)	474
BsrI	ACTGG	2 cut(s)	133, 1891
BssAI	RCCGGY	1 cut(s)	1875
BssECI	CCNNGG	8 cut(s)	343, 344, 370, 883, 1296, 1452, 1708, 1861
BssMI	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
BssSI	CACGAG	2 cut(s)	215, 583
BssT1I	CCWWGG	1 cut(s)	1296
Bst2BI	CACGAG	2 cut(s)	215, 583
Bst2UI	CCWGG	6 cut(s)	830, 1205, 1260, 1454, 1566, 1710
Bst4CI	ACNGT	7 cut(s)	263, 326, 1019, 1038, 1498, 1897, 2110
Bst6I	CTCTTC	3 cut(s)	215, 541, 969
BstAPI	GCANNNNNTGC	2 cut(s)	1362, 1490
BstAUI	TGTACA	1 cut(s)	474
BstBAI	YACGTR	1 cut(s)	1217
BstBI	TTCGAA	1 cut(s)	359
BstC8I	GCNNGC	6 cut(s)	145, 268, 281, 1154, 1212, 2234
BstDEI	CTNAG	9 cut(s)	121, 390, 459, 987, 1030, 1074, 1978, 2186, 2193
BstEII	GGTNACC	2 cut(s)	1703, 1856
BstENI	CCTNNNNNAGG	1 cut(s)	1383
BstF5I	GGATG	3 cut(s)	760, 1189, 2230
BstHHI	GCGC	2 cut(s)	854, 1430
BstKTI	GATC	8 cut(s)	599, 1012, 1025, 1147, 1265, 1846, 1990, 2287
BstMAI	GTCTC	8 cut(s)	525, 551, 630, 1222, 1281, 1523, 1831, 2298
BstMBI	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
BstMCI	CGRYCG	1 cut(s)	195
BstNI	CCWGG	6 cut(s)	830, 1205, 1260, 1454, 1566, 1710
BstNSI	RCATGY	2 cut(s)	666, 2300
BstPI	GGTNACC	2 cut(s)	1703, 1856
BstSFI	CTRYAG	1 cut(s)	981
BstSLI	GKGCMC	2 cut(s)	1193, 2142
BstV1I	GCAGC	9 cut(s)	174, 419, 671, 967, 995, 1091, 1490, 1496, 1714
BstV2I	GAAGAC	3 cut(s)	576, 608, 1777
BstX2I	RGATCY	2 cut(s)	1009, 1843
BstXI	CCANNNNNNTGG	1 cut(s)	836
BstYI	RGATCY	2 cut(s)	1009, 1843
BstZI	CGGCCG	1 cut(s)	192
BsuI	GTATCC	4 cut(s)	596, 899, 1800, 1904
BtrI	CACGTC	2 cut(s)	1741, 1785
BtsCI	GGATG	3 cut(s)	760, 1189, 2230
BtsI	GCAGTG	6 cut(s)	63, 277, 738, 1059, 1265, 1446
BtsIMutI	CAGTG	8 cut(s)	63, 268, 277, 738, 1059, 1265, 1446, 1884
BveI	ACCTGC	2 cut(s)	857, 992
Cac8I	GCNNGC	6 cut(s)	145, 268, 281, 1154, 1212, 2234
CaiI	CAGNNNCTG	2 cut(s)	1210, 1847
CciI	TCATGA	1 cut(s)	1540
CfoI	GCGC	2 cut(s)	854, 1430
Cfr10I	RCCGGY	1 cut(s)	1875
Cfr13I	GGNCC	8 cut(s)	211, 341, 348, 507, 1175, 1310, 1613, 1813
Cfr9I	CCCGGG	2 cut(s)	344, 1861
CseI	GACGC	2 cut(s)	399, 1112
CsiI	ACCWGGT	1 cut(s)	828
Csp6I	GTAC	8 cut(s)	41, 298, 334, 475, 940, 1039, 1499, 2291
CspAI	ACCGGT	1 cut(s)	1875
CspCI	CAANNNNNGTGG	6 cut(s)	1396, 1398, 1431, 1433, 2077, 2112
CviAII	CATG	9 cut(s)	663, 751, 814, 1541, 1828, 1991, 2083, 2288, 2297
CviQI	GTAC	8 cut(s)	41, 298, 334, 475, 940, 1039, 1499, 2291
DdeI	CTNAG	9 cut(s)	121, 390, 459, 987, 1030, 1074, 1978, 2186, 2193
DpnI	GATC	8 cut(s)	598, 1011, 1024, 1146, 1264, 1845, 1989, 2286
DpnII	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
DraIII	CACNNNGTG	1 cut(s)	328
DrdI	GACNNNNNNGTC	3 cut(s)	409, 505, 1173
DriI	GACNNNNNGTC	1 cut(s)	1536
DseDI	GACNNNNNNGTC	3 cut(s)	409, 505, 1173
EaeI	YGGCCR	3 cut(s)	130, 192, 1419
EagI	CGGCCG	1 cut(s)	192
Eam1104I	CTCTTC	3 cut(s)	215, 541, 969
Eam1105I	GACNNNNNGTC	1 cut(s)	1536
EarI	CTCTTC	3 cut(s)	215, 541, 969
EciI	GGCGGA	1 cut(s)	224
Ecl136II	GAGCTC	1 cut(s)	120
EclXI	CGGCCG	1 cut(s)	192
Eco130I	CCWWGG	1 cut(s)	1296
Eco147I	AGGCCT	2 cut(s)	68, 1389
Eco24I	GRGCYC	1 cut(s)	122
Eco31I	GGTCTC	2 cut(s)	525, 1831
Eco32I	GATATC	1 cut(s)	2062
Eco47I	GGWCC	7 cut(s)	211, 348, 507, 1175, 1310, 1613, 1813
Eco52I	CGGCCG	1 cut(s)	192
Eco53kI	GAGCTC	1 cut(s)	120
Eco57I	CTGAAG	1 cut(s)	1611
Eco72I	CACGTG	1 cut(s)	1217
Eco88I	CYCGRG	3 cut(s)	344, 535, 1861
Eco91I	GGTNACC	2 cut(s)	1703, 1856
EcoICRI	GAGCTC	1 cut(s)	120
EcoNI	CCTNNNNNAGG	1 cut(s)	1383
EcoO109I	RGGNCCY	3 cut(s)	1310, 1613, 1813
EcoO65I	GGTNACC	2 cut(s)	1703, 1856
EcoRI	GAATTC	1 cut(s)	288
EcoRII	CCWGG	6 cut(s)	828, 1203, 1258, 1452, 1564, 1708
EcoRV	GATATC	1 cut(s)	2062
EcoT14I	CCWWGG	1 cut(s)	1296
EcoT38I	GRGCYC	1 cut(s)	122
ErhI	CCWWGG	1 cut(s)	1296
FaeI	CATG	9 cut(s)	666, 754, 817, 1544, 1831, 1994, 2086, 2291, 2300
FalI	AAGNNNNNCTT	2 cut(s)	1050, 1082
FaqI	GGGAC	5 cut(s)	353, 361, 493, 1296, 1799
FatI	CATG	9 cut(s)	662, 750, 813, 1540, 1827, 1990, 2082, 2287, 2296
FauNDI	CATATG	2 cut(s)	1162, 1801
FblI	GTMKAC	1 cut(s)	1806
FokI	GGATG	3 cut(s)	767, 1196, 2237
FriOI	GRGCYC	1 cut(s)	122
FspBI	CTAG	5 cut(s)	807, 1127, 1946, 2051, 2258
FspI	TGCGCA	1 cut(s)	853
GlaI	GCGC	2 cut(s)	853, 1429
GsuI	CTGGAG	2 cut(s)	1242, 1659
HapII	CCGG	7 cut(s)	129, 345, 372, 563, 1422, 1862, 1876
HgaI	GACGC	2 cut(s)	399, 1112
HhaI	GCGC	2 cut(s)	854, 1430
Hin1II	CATG	9 cut(s)	666, 754, 817, 1544, 1831, 1994, 2086, 2291, 2300
Hin6I	GCGC	2 cut(s)	852, 1428
HinP1I	GCGC	2 cut(s)	852, 1428
HincII	GTYRAC	1 cut(s)	456
HindII	GTYRAC	1 cut(s)	456
HinfI	GANTC	4 cut(s)	559, 926, 1548, 2125
HpaII	CCGG	7 cut(s)	129, 345, 372, 563, 1422, 1862, 1876
HphI	GGTGA	6 cut(s)	268, 1154, 1523, 1901, 1931, 2086
Hpy166II	GTNNAC	8 cut(s)	259, 456, 671, 940, 1041, 1191, 1807, 2140
Hpy188I	TCNGA	5 cut(s)	33, 313, 443, 530, 884
Hpy188III	TCNNGA	8 cut(s)	217, 583, 600, 791, 1249, 1541, 1638, 2051
Hpy8I	GTNNAC	8 cut(s)	259, 456, 671, 940, 1041, 1191, 1807, 2140
Hpy99I	CGWCG	2 cut(s)	341, 415
HpyAV	CCTTC	8 cut(s)	58, 438, 875, 1040, 1346, 1594, 1942, 2170
HpyCH4III	ACNGT	7 cut(s)	263, 326, 1019, 1038, 1498, 1897, 2110
HpyCH4IV	ACGT	6 cut(s)	248, 336, 606, 1216, 1740, 1784
HpyF3I	CTNAG	9 cut(s)	121, 390, 459, 987, 1030, 1074, 1978, 2186, 2193
HpySE526I	ACGT	6 cut(s)	248, 336, 606, 1216, 1740, 1784
Hsp92II	CATG	9 cut(s)	666, 754, 817, 1544, 1831, 1994, 2086, 2291, 2300
HspAI	GCGC	2 cut(s)	852, 1428
Kzo9I	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
LmnI	GCTCC	4 cut(s)	374, 1049, 1207, 1724
Lsp1109I	GCAGC	9 cut(s)	174, 419, 671, 967, 995, 1091, 1490, 1496, 1714
LweI	GCATC	5 cut(s)	187, 1110, 1453, 1480, 1663
MabI	ACCWGGT	1 cut(s)	828
MaeI	CTAG	5 cut(s)	807, 1127, 1946, 2051, 2258
MaeII	ACGT	6 cut(s)	248, 336, 606, 1216, 1740, 1784
MaeIII	GTNAC	9 cut(s)	89, 587, 736, 913, 1069, 1324, 1703, 1856, 1889
MalI	GATC	8 cut(s)	598, 1011, 1024, 1146, 1264, 1845, 1989, 2286
MboI	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
MfeI	CAATTG	2 cut(s)	697, 1524
MflI	RGATCY	2 cut(s)	1009, 1843
MhlI	GDGCHC	4 cut(s)	122, 1193, 1957, 2142
MluCI	AATT	7 cut(s)	288, 697, 1105, 1524, 1680, 1904, 2143
MlyI	GAGTC	2 cut(s)	553, 2134
MmeI	TCCRAC	1 cut(s)	291
MroXI	GAANNNNTTC	1 cut(s)	2180
MseI	TTAA	3 cut(s)	519, 651, 834
MslI	CAYNNNNRTG	5 cut(s)	98, 726, 1118, 1832, 2241
MspI	CCGG	7 cut(s)	129, 345, 372, 563, 1422, 1862, 1876
MunI	CAATTG	2 cut(s)	697, 1524
Mva1269I	GAATGC	1 cut(s)	1689
MvaI	CCWGG	6 cut(s)	830, 1205, 1260, 1454, 1566, 1710
NciI	CCSGG	7 cut(s)	345, 346, 372, 564, 1423, 1862, 1863
NdeI	CATATG	2 cut(s)	1162, 1801
NdeII	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
NlaIII	CATG	9 cut(s)	666, 754, 817, 1544, 1831, 1994, 2086, 2291, 2300
NlaIV	GGNNCC	7 cut(s)	157, 343, 349, 509, 1312, 1357, 1815
NmuCI	GTSAC	4 cut(s)	913, 1069, 1324, 1889
NsbI	TGCGCA	1 cut(s)	853
NspI	RCATGY	2 cut(s)	666, 2300
NspV	TTCGAA	1 cut(s)	359
OliI	CACNNNNGTG	1 cut(s)	2241
PagI	TCATGA	1 cut(s)	1540
PceI	AGGCCT	2 cut(s)	68, 1389
PciI	ACATGT	1 cut(s)	2296
PctI	GAATGC	1 cut(s)	1689
PdmI	GAANNNNTTC	1 cut(s)	2180
PfeI	GAWTC	2 cut(s)	926, 1548
Pfl23II	CGTACG	1 cut(s)	333
PflFI	GACNNNGTC	1 cut(s)	1811
PflMI	CCANNNNNTGG	1 cut(s)	1210
PfoI	TCCNGGA	3 cut(s)	562, 1258, 1564
PinAI	ACCGGT	1 cut(s)	1875
PleI	GAGTC	2 cut(s)	553, 2133
PmaCI	CACGTG	1 cut(s)	1217
PmlI	CACGTG	1 cut(s)	1217
PpsI	GAGTC	2 cut(s)	553, 2133
Ppu21I	YACGTR	1 cut(s)	1217
PpuMI	RGGWCCY	3 cut(s)	1310, 1613, 1813
PscI	ACATGT	1 cut(s)	2296
PsiI	TTATAA	1 cut(s)	1758
Psp124BI	GAGCTC	1 cut(s)	122
Psp5II	RGGWCCY	3 cut(s)	1310, 1613, 1813
Psp6I	CCWGG	6 cut(s)	828, 1203, 1258, 1452, 1564, 1708
PspCI	CACGTG	1 cut(s)	1217
PspEI	GGTNACC	2 cut(s)	1703, 1856
PspGI	CCWGG	6 cut(s)	828, 1203, 1258, 1452, 1564, 1708
PspLI	CGTACG	1 cut(s)	333
PspN4I	GGNNCC	7 cut(s)	157, 343, 349, 509, 1312, 1357, 1815
PspPI	GGNCC	8 cut(s)	211, 341, 348, 507, 1175, 1310, 1613, 1813
PspPPI	RGGWCCY	3 cut(s)	1310, 1613, 1813
PsrI	GAACNNNNNNTAC	2 cut(s)	941, 973
PstI	CTGCAG	1 cut(s)	985
PstNI	CAGNNNCTG	2 cut(s)	1210, 1847
PsuI	RGATCY	2 cut(s)	1009, 1843
PsyI	GACNNNGTC	1 cut(s)	1811
RsaI	GTAC	8 cut(s)	42, 299, 335, 476, 941, 1040, 1500, 2292
RsaNI	GTAC	8 cut(s)	41, 298, 334, 475, 940, 1039, 1499, 2291
RseI	CAYNNNNRTG	5 cut(s)	98, 726, 1118, 1832, 2241
SacI	GAGCTC	1 cut(s)	122
SaqAI	TTAA	3 cut(s)	519, 651, 834
Sau3AI	GATC	8 cut(s)	596, 1009, 1022, 1144, 1262, 1843, 1987, 2284
Sau96I	GGNCC	8 cut(s)	211, 341, 348, 507, 1175, 1310, 1613, 1813
ScaI	AGTACT	1 cut(s)	42
SchI	GAGTC	2 cut(s)	553, 2134
SduI	GDGCHC	4 cut(s)	122, 1193, 1957, 2142
SexAI	ACCWGGT	1 cut(s)	828
SfaNI	GCATC	5 cut(s)	187, 1110, 1453, 1480, 1663
SfcI	CTRYAG	1 cut(s)	981
SfuI	TTCGAA	1 cut(s)	359
SinI	GGWCC	7 cut(s)	211, 348, 507, 1175, 1310, 1613, 1813
SmaI	CCCGGG	2 cut(s)	346, 1863
SmiMI	CAYNNNNRTG	5 cut(s)	98, 726, 1118, 1832, 2241
SmlI	CTYRAG	3 cut(s)	1247, 1794, 2239
SmoI	CTYRAG	3 cut(s)	1247, 1794, 2239
Sse9I	AATT	7 cut(s)	288, 697, 1105, 1524, 1680, 1904, 2143
SseBI	AGGCCT	2 cut(s)	68, 1389
SsiI	CCGC	7 cut(s)	147, 191, 209, 405, 633, 1482, 1942
SspMI	CTAG	5 cut(s)	807, 1127, 1946, 2051, 2258
SstI	GAGCTC	1 cut(s)	122
StuI	AGGCCT	2 cut(s)	68, 1389
StyI	CCWWGG	1 cut(s)	1296
TaaI	ACNGT	7 cut(s)	263, 326, 1019, 1038, 1498, 1897, 2110
TaiI	ACGT	6 cut(s)	251, 339, 609, 1219, 1743, 1787
TaqI	TCGA	7 cut(s)	359, 1137, 1551, 1650, 1951, 2058, 2123
TasI	AATT	7 cut(s)	288, 697, 1105, 1524, 1680, 1904, 2143
TatI	WGTACW	6 cut(s)	40, 474, 939, 1038, 1498, 2290
TauI	GCSGC	2 cut(s)	194, 1484
TfiI	GAWTC	2 cut(s)	926, 1548
Tru1I	TTAA	3 cut(s)	519, 651, 834
Tru9I	TTAA	3 cut(s)	519, 651, 834
TscAI	CASTG	8 cut(s)	63, 268, 277, 738, 1059, 1272, 1453, 1891
TseFI	GTSAC	4 cut(s)	913, 1069, 1324, 1889
TseI	GCWGC	9 cut(s)	162, 407, 659, 980, 983, 1079, 1478, 1484, 1727
Tsp45I	GTSAC	4 cut(s)	913, 1069, 1324, 1889
TspDTI	ATGAA	2 cut(s)	1844, 1917
TspGWI	ACGGA	2 cut(s)	609, 1929
TspMI	CCCGGG	2 cut(s)	344, 1861
TspRI	CASTG	8 cut(s)	63, 268, 277, 738, 1059, 1272, 1453, 1891
Tth111I	GACNNNGTC	1 cut(s)	1811
Van91I	CCANNNNNTGG	1 cut(s)	1210
VneI	GTGCAC	2 cut(s)	1189, 2138
VpaK11BI	GGWCC	7 cut(s)	211, 348, 507, 1175, 1310, 1613, 1813
XagI	CCTNNNNNAGG	1 cut(s)	1383
XapI	RAATTY	2 cut(s)	288, 1904
XbaI	TCTAGA	1 cut(s)	2050
XceI	RCATGY	2 cut(s)	666, 2300
XcmI	CCANNNNNNNNNTGG	3 cut(s)	445, 1294, 1414
XmaI	CCCGGG	2 cut(s)	344, 1861
XmiI	GTMKAC	1 cut(s)	1806
XmnI	GAANNNNTTC	1 cut(s)	2180
XspI	CTAG	5 cut(s)	807, 1127, 1946, 2051, 2258
ZrmI	AGTACT	1 cut(s)	42

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.