Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004171

Physical Location & Seq

Genomic Coordinates Reverse (-)

4148887 .. 4155043

Gene Structure 6157 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1060800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGGCGACCCCGAAACGCCAGCGGTCCCGGGAGGAGTTGGAGGACATAGTCCTCCGCAAAATCTTTCTCGTATCGCTGACCGGTCCATCGGACTCCGATTCCCGAGTCGTTTACTTGGAGATGACTGCGGCAGAGATTCTCAGCGAGGGGAAGGAGTTGAGGCTCAGTTGCGACCTCATGGAATCGATTCTAATCGATCGGCTATCCGGCAGCTTCCCCGCTGCTGAACTGCCGTTCCAGTACCTGATCGGGTGTTACAAACGCGCCTACGACGAGGGTAAGAAAATTGCTTCCATGAAAGATAGGAACTTGAAGTCGGAATTGGAATCCGTGGTTAGGCAAGCGAAGAAGCTTTCGGTTTCGTATTGTAGGATTCATTTGGGGAATCCCGAATCGTTCCCCAACCCGAATTTCGATTCGAACAAGCCGAATGCCTCGCCTTTATTGCCATTGATCTTCTCAGAGGGTGGCGGTTCAGCTGATGGGTTTGGAGGAAGTGGGAGTAGTGGGGGAATTCAGTGCCCACCTGGGTTTTTGGAGGAGTTCTTCACCGACTCCGATTTCGATAGCTTGGACCCAATTTTGAAGGGATTGTATGAGGAGCTTAGAGAGATTGTGCTTAAGGTTTCGGCCCTCGGAAACTTCCAGCAGCCACTGAGGGCTCTGTATTTTTTGGTTAAGTTGCCGGTTGGTGCAAGGTCACTTGTGAATCACCCGTGGTGGATTCCAAAAGGGGTGTACTTGAATGGGCGTGTGATTGAGAGGACTAGCATTTTGGGTCCTTTTTTTCATGTCAGTGCTCTGCCTGATCATCCTATTTTCAAGAGCCAGCCTGATGTTGGGTTGCGATTATTTGCATTTGTTGTTGACATGGTCTTCTTGTTATGTTATTTCCTTTGGCAGCAGTGCTTTTCAGAAGCTTCAACTCGTAGACCAGCTGATCTGCTATCTTCATTTGCCACAATTAAGACAGTCATGAATAACTTATATGATGGCCTTACTGAAGTTCTTCTCTTACTACTGAAAAATGCTGACACCCGTGAGAATGTTCTTGAGTATCTTGCAGAGGTGATCAACAAAAACTCATCAAGGGCTCAGATTCAGGTAGATCCTCTTTCATGTGCAAGTTCAGGCATGTTTGTCAATCTCAGTGCTGTCATGCTTCGGCTTTGTGAGCCGTTTTTAGATGCAAATTTAACAAAAAGAGACAAAATTGATCCAAAATATGTATTCTACAGTAACCGTTTGGAGTTAAGAGGTTTGACTGCTTTACATGCATCGTCTGAAGAAGTTACTGAATGGATTAATAAAGCTAGTATGGGGAATACTGATGGTGAAACTCGCCTTTTACAATCTCAAGAAGCCACCAGTTCCGGAAATAGTGTCAACGTGAAGCCAAATAGTGAAAAAGCTAAATATTCATTTATTTGCGAATGCTTCTTCATGACTGCAAGGGTGCTCAATCTGGGATTGTTGAAAGCATTTTCTGACTTTAAACATTTAGTACAGGACATTTCAAGGTCTGAAGATACTCTCACCACACTTAAAGCCATGCAAGGCCAGACATCTTCTCCACAATTGGAGATAGATATAGCTCGCCTTGAGAAGGAAATAGAGTCATATTCGCAGGAAAAGCTTTCTTATGAAGCTCAGATATTAAGGGATCCGACAGTTATACAGAGTGCTCTTACTTTCTATCGTTTGATGGTGGTGTGGCTAGTTCGCTTGGTTGGTGGGTTTAAAATGCCCCTGCCATCAACTAGCCCTACGGAGTTTGCTTCTATGCCAGAGCATTTTGTGGAAGATGCCATGGAGTTGCTTATATTTGCTTCCCGGATTCCAAAAGCCTTGGATGGGGTTTTGTTGGATGATTTTATGAACTTCATTATAATGTTTATGGCCAGCCCAGAATATATCAGGAACCCTTACCTAAGAGCAAAGATGGTTGAAGTCCTAAACTGCTGGATGCCCCGTGGAAGCGGATCATCTATTACAGCGACCCTATTTGAAGGGCACCAACTATCTCTTGAGTATCTTGTGAGGAATCTCTTGAAGCTATATGTTGACATCGAGTTCACTGGTTCTCACACACAGTTCTATGACAAGTTTAATATTCGCCACAATATTGCTGAACTTCTTGAATATCTGTGGCATGTCCCTAGTCATCGAAATGCTTGGAAACAGATTGCAAAGGAAGAGGAGAAGGGTGTTTACTTGAATTTCTTAAACTTCTTGATCAATGATAGCATCTATCTTCTGGATGAAAGTCTTAACAAAATTCTTGAACACAAAGAACTGGAAGCTGAGATGTCAAACACTGCAGAGTGGGAGCGCAGACCTGCTCAAGAAAGGCAGGAGAGAACACGCCTCTTCCAGTCTCAAGAGAATATTATCCGAATAGATATGAAGTTAGCAAATGAAGACGTTAGTATGATGGCATTTACTACAGAACAAATTACAGCTCCTTTCCTACTTCCTGAGATGGTTGAAAGGGTGGCCAGCATGCTCAATTACTTTTTGTTGCAATTAGTAGGCCCTCAAAGAAAATCTCTTAGCTTGAAAGACCCTGAGAAGTATGAATTCCGTCCAAAGCAATTGCTTAAGCAGATTGTATACATATATGTTCATTTGGCAAAGGGTGATTCAGAAAATATATTTCCAGCTGCCATCTCAAAGGATGGTCGTTCGTACAATGAGCAGTTATTTAGTGCTGCAGCAGATGTCCTAAGGAAAATTGGTGAAGATGGGAGGATCATACAAGAGTTTATTGAGCTAGGTGCGAAAGCCAAGGTTGCTGCTTCGGAGGCCATGGACATTGAAGCTATTCTTGGGGACATACCAGATGAATTCCTCGATCCAATTCAGTACACTTTAATGAAGGATCCAGTTATCTTACCTTCTTCAAGAATCACAGTAGACCGACCCGTCATTCAAAGGCATCTTCTTAGTGATAGTTCTGATCCGTTCAACCGATCCCATCTTACTGCGGACATGCTGATACCGGACAATGAGTTAAAGGCAAGGATACAAGAGTTCATTAGGTCCCAAGAATCGAAGAAGCGCGGGGAAGACTTGAGCACGCAGAGCTCCAAGGCAACGATACAAACTACAACTAGTGAAATGCTAATTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0030433 GO:0032446 GO:0033554 GO:0034450 GO:0034976 GO:0036211 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04120 ko04141 map04120 map04141

Pfam Domains

Protein Families

U-box Ufd2P_core

Protein Analysis

1045

Amino Acids

118.44

Weight (kDa)

5.35

Isoelectric Point (pI)

50.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	259 - 929	3.9e-201	Ubiquitin elongating factor core
U-box	PF04564	946 - 1017	4.9e-30	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15400
fragaria_vesca	FvH4_3g25920 FvH4_3g25930 FvH4_6g47550 FvH4_6g47550 FvH4_6g47550
malus_domestica	MD09G1060800.v1.1 MD17G1056200.v1.1
prunus_persica	Prupe.3G259400_v2.0.a1
pyrus_communis	pycom111g05110 pycom17g05530
rosa_chinensis	RchiOBHm_Chr2g0166651 RchiOBHm_Chr5g0047221
rosa_laevigata	RLG00000021638 RLG00000034480
rosa_multiflora	Rmu_sc0002310.1_g000029 Rmu_sc0005232.1_g000002
rosa_roxburghii	Rroxscaffold_1G00034160 Rroxscaffold_2G00084330 Rroxscaffold_2G00084640
rosa_rugosa	Rorug02G0524700 Rorug05G0235100
rosa_samantha	Rh2AG590900 Rh2BG602800 Rh2CG573700 Rh2DG613600 Rh5AG314000 Rh5BG322500 Rh5CG349700 Rh5CG349800 Rh5DG333500
rosa_wichuraiana	Rw2G049220 Rw5G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1889
AasI	GACNNNNNNGTC	1 cut(s)	2924
Acc36I	ACCTGC	1 cut(s)	2347
AccB1I	GGYRCC	1 cut(s)	2013
AccI	GTMKAC	3 cut(s)	931, 2613, 2916
AccII	CGCG	2 cut(s)	264, 3063
AccIII	TCCGGA	1 cut(s)	1373
AciI	CCGC	8 cut(s)	22, 55, 128, 219, 471, 1980, 2987, 3063
AcoI	YGGCCR	2 cut(s)	1899, 2496
AcsI	RAATTY	7 cut(s)	409, 513, 1192, 2218, 2277, 2579, 2846
AcuI	CTGAAG	3 cut(s)	1023, 1305, 1545
AfaI	GTAC	5 cut(s)	242, 740, 1506, 2690, 2867
AfiI	CCNNNNNNNGG	4 cut(s)	336, 839, 1606, 1854
AflII	CTTAAG	2 cut(s)	620, 2600
AgeI	ACCGGT	1 cut(s)	80
AhlI	ACTAGT	1 cut(s)	3113
AjnI	CCWGG	1 cut(s)	526
AjuI	GAANNNNNNNTTGG	6 cut(s)	305, 337, 395, 427, 2811, 2843
AloI	GAACNNNNNNTCC	2 cut(s)	219, 251
Alw21I	GWGCWC	5 cut(s)	802, 1461, 1687, 3080, 3089
Alw26I	GTCTC	2 cut(s)	1200, 2382
AlwNI	CAGNNNCTG	2 cut(s)	244, 655
Ama87I	CYCGRG	2 cut(s)	27, 102
Aor13HI	TCCGGA	1 cut(s)	1373
AoxI	GGCC	7 cut(s)	630, 994, 1558, 1899, 2496, 2533, 2805
ApeKI	GCWGC	8 cut(s)	210, 221, 649, 901, 2663, 2711, 2714, 2795
ApoI	RAATTY	7 cut(s)	409, 513, 1192, 2218, 2277, 2579, 2846
ArsI	GACNNNNNNTTYG	4 cut(s)	545, 577, 757, 789
AseI	ATTAAT	1 cut(s)	1305
AsiGI	ACCGGT	1 cut(s)	80
Asp700I	GAANNNNTTC	3 cut(s)	186, 1008, 2823
AspLEI	GCGC	3 cut(s)	266, 2334, 3063
AspS9I	GGNCC	7 cut(s)	24, 83, 574, 631, 779, 2534, 3042
AsuC2I	CCSGG	3 cut(s)	28, 29, 1834
AsuHPI	GGTGA	7 cut(s)	541, 704, 1081, 1346, 1528, 2651, 2750
AsuII	TTCGAA	1 cut(s)	419
AvaI	CYCGRG	2 cut(s)	27, 102
AvaII	GGWCC	5 cut(s)	24, 83, 574, 779, 3042
AxyI	CCTNAGG	1 cut(s)	2726
BaeGI	GKGCMC	2 cut(s)	524, 2016
BaeI	ACNNNNGTAYC	2 cut(s)	3017, 3050
BalI	TGGCCA	2 cut(s)	1901, 2498
BamHI	GGATCC	2 cut(s)	1663, 2881
BanI	GGYRCC	1 cut(s)	2013
BanII	GRGCYC	3 cut(s)	664, 1096, 3089
BarI	GAAGNNNNNNTAC	2 cut(s)	2195, 2227
BbsI	GAAGAC	3 cut(s)	868, 2427, 3075
Bbv12I	GWGCWC	5 cut(s)	802, 1461, 1687, 3080, 3089
BbvI	GCAGC	8 cut(s)	208, 222, 661, 913, 2650, 2698, 2726, 2782
BceAI	ACGGC	2 cut(s)	217, 1162
BciT130I	CCWGG	1 cut(s)	528
BciVI	GTATCC	1 cut(s)	3018
BclI	TGATCA	3 cut(s)	808, 1071, 2235
BcnI	CCSGG	3 cut(s)	28, 29, 1834
BcoDI	GTCTC	2 cut(s)	1200, 2382
BcuI	ACTAGT	1 cut(s)	3113
BfaI	CTAG	7 cut(s)	768, 1314, 1718, 1761, 2160, 2774, 3114
BfmI	CTRYAG	4 cut(s)	1234, 2319, 2445, 2712
BfrI	CTTAAG	2 cut(s)	620, 2600
BfuAI	ACCTGC	1 cut(s)	2347
BfuI	GTATCC	1 cut(s)	3018
BisI	GCNGC	9 cut(s)	129, 211, 222, 650, 902, 2664, 2712, 2715, 2796
BlsI	GCNGC	9 cut(s)	130, 212, 223, 651, 903, 2665, 2713, 2716, 2797
Bme1390I	CCNGG	4 cut(s)	28, 29, 528, 1834
Bme18I	GGWCC	5 cut(s)	24, 83, 574, 779, 3042
BmeT110I	CYCGRG	2 cut(s)	27, 102
BmgT120I	GGNCC	7 cut(s)	24, 83, 574, 631, 779, 2534, 3042
BmiI	GGNNCC	8 cut(s)	26, 576, 780, 1665, 1922, 2015, 2883, 3044
BmrFI	CCNGG	4 cut(s)	28, 29, 528, 1834
BmsI	GCATC	6 cut(s)	1177, 1286, 1795, 1956, 2256, 2947
BpiI	GAAGAC	3 cut(s)	868, 2427, 3075
Bpu14I	TTCGAA	1 cut(s)	419
BpuEI	CTTGAG	7 cut(s)	1073, 1341, 1622, 2048, 2328, 2364, 3094
BpuMI	CCSGG	3 cut(s)	28, 29, 1834
Bsa29I	ATCGAT	2 cut(s)	185, 195
BsaBI	GATNNNNATC	1 cut(s)	191
BsaWI	WCCGGW	3 cut(s)	80, 1373, 3001
BsaXI	ACNNNNNCTCC	2 cut(s)	1555, 1585
Bsc4I	CCNNNNNNNGG	4 cut(s)	336, 839, 1606, 1854
Bse118I	RCCGGY	2 cut(s)	80, 685
Bse1I	ACTGG	6 cut(s)	238, 1368, 2083, 2301, 2374, 2885
Bse21I	CCTNAGG	1 cut(s)	2726
Bse8I	GATNNNNATC	1 cut(s)	191
BseAI	TCCGGA	1 cut(s)	1373
BseBI	CCWGG	1 cut(s)	528
BseCI	ATCGAT	2 cut(s)	185, 195
BseGI	GGATG	6 cut(s)	811, 1858, 1873, 1971, 2266, 2683
BseJI	GATNNNNATC	1 cut(s)	191
BseLI	CCNNNNNNNGG	4 cut(s)	336, 839, 1606, 1854
BseNI	ACTGG	6 cut(s)	238, 1368, 2083, 2301, 2374, 2885
BseRI	GAGGAG	4 cut(s)	47, 554, 614, 2213
BseSI	GKGCMC	2 cut(s)	524, 2016
BseXI	GCAGC	8 cut(s)	208, 222, 661, 913, 2650, 2698, 2726, 2782
Bsh1236I	CGCG	2 cut(s)	264, 3063
Bsh1285I	CGRYCG	1 cut(s)	199
BshFI	GGCC	7 cut(s)	632, 996, 1560, 1901, 2498, 2535, 2807
BshNI	GGYRCC	1 cut(s)	2013
BshTI	ACCGGT	1 cut(s)	80
BshVI	ATCGAT	2 cut(s)	185, 195
BsiEI	CGRYCG	1 cut(s)	199
BsiHKAI	GWGCWC	5 cut(s)	802, 1461, 1687, 3080, 3089
BsiHKCI	CYCGRG	2 cut(s)	27, 102
BsiSI	CCGG	7 cut(s)	28, 81, 207, 686, 1374, 1834, 3002
BslFI	GGGAC	4 cut(s)	10, 2141, 2846, 3028
BslI	CCNNNNNNNGG	4 cut(s)	336, 839, 1606, 1854
BsmAI	GTCTC	2 cut(s)	1200, 2382
BsmFI	GGGAC	4 cut(s)	10, 2141, 2846, 3028
BsmI	GAATGC	2 cut(s)	436, 1439
BsnI	GGCC	7 cut(s)	632, 996, 1560, 1901, 2498, 2535, 2807
BsoBI	CYCGRG	2 cut(s)	27, 102
Bsp119I	TTCGAA	1 cut(s)	419
Bsp1286I	GDGCHC	9 cut(s)	524, 664, 802, 1096, 1461, 1687, 2016, 3080, 3089
Bsp13I	TCCGGA	1 cut(s)	1373
Bsp19I	CCATGG	2 cut(s)	1809, 2808
BspACI	CCGC	8 cut(s)	22, 55, 128, 219, 471, 1980, 2987, 3063
BspANI	GGCC	7 cut(s)	632, 996, 1560, 1901, 2498, 2535, 2807
BspDI	ATCGAT	2 cut(s)	185, 195
BspEI	TCCGGA	1 cut(s)	1373
BspFNI	CGCG	2 cut(s)	264, 3063
BspHI	TCATGA	2 cut(s)	975, 1443
BspLI	GGNNCC	8 cut(s)	26, 576, 780, 1665, 1922, 2015, 2883, 3044
BspMAI	CTGCAG	2 cut(s)	2323, 2716
BspMI	ACCTGC	1 cut(s)	2347
BspT104I	TTCGAA	1 cut(s)	419
BspT107I	GGYRCC	1 cut(s)	2013
BspTI	CTTAAG	2 cut(s)	620, 2600
BsrFI	RCCGGY	2 cut(s)	80, 685
BsrI	ACTGG	6 cut(s)	238, 1368, 2083, 2301, 2374, 2885
BssAI	RCCGGY	2 cut(s)	80, 685
BssNAI	GTATAC	1 cut(s)	2614
BssT1I	CCWWGG	5 cut(s)	1809, 1848, 2787, 2808, 3090
Bst1107I	GTATAC	1 cut(s)	2614
Bst2UI	CCWGG	1 cut(s)	528
Bst4CI	ACNGT	6 cut(s)	973, 1238, 1244, 1672, 2094, 2914
Bst6I	CTCTTC	2 cut(s)	2190, 2375
BstAFI	CTTAAG	2 cut(s)	620, 2600
BstBI	TTCGAA	1 cut(s)	419
BstC8I	GCNNGC	8 cut(s)	20, 342, 830, 1597, 1903, 2500, 2504, 3080
BstDSI	CCRYGG	5 cut(s)	330, 716, 1809, 1972, 2808
BstENI	CCTNNNNNAGG	1 cut(s)	1604
BstF5I	GGATG	6 cut(s)	811, 1858, 1873, 1971, 2266, 2683
BstFNI	CGCG	2 cut(s)	264, 3063
BstHHI	GCGC	3 cut(s)	266, 2334, 3063
BstMAI	GTCTC	2 cut(s)	1200, 2382
BstMCI	CGRYCG	1 cut(s)	199
BstMWI	GCNNNNNNNGC	7 cut(s)	445, 1174, 1274, 1633, 2792, 2804, 3084
BstNI	CCWGG	1 cut(s)	528
BstNSI	RCATGY	5 cut(s)	1138, 1277, 2156, 2506, 2995
BstSCI	CCNGG	4 cut(s)	26, 27, 526, 1832
BstSFI	CTRYAG	4 cut(s)	1234, 2319, 2445, 2712
BstSLI	GKGCMC	2 cut(s)	524, 2016
BstUI	CGCG	2 cut(s)	264, 3063
BstV1I	GCAGC	8 cut(s)	208, 222, 661, 913, 2650, 2698, 2726, 2782
BstV2I	GAAGAC	3 cut(s)	868, 2427, 3075
BstX2I	RGATCY	3 cut(s)	1108, 1663, 2881
BstYI	RGATCY	3 cut(s)	1108, 1663, 2881
BstZ17I	GTATAC	1 cut(s)	2614
Bsu15I	ATCGAT	2 cut(s)	185, 195
Bsu36I	CCTNAGG	1 cut(s)	2726
BsuI	GTATCC	1 cut(s)	3018
BsuRI	GGCC	7 cut(s)	632, 996, 1560, 1901, 2498, 2535, 2807
BsuTUI	ATCGAT	2 cut(s)	185, 195
BtgI	CCRYGG	5 cut(s)	330, 716, 1809, 1972, 2808
BtsCI	GGATG	6 cut(s)	811, 1858, 1873, 1971, 2266, 2683
BtsI	GCAGTG	2 cut(s)	911, 2316
BtsIMutI	CAGTG	7 cut(s)	524, 653, 802, 911, 1156, 2076, 2316
BveI	ACCTGC	1 cut(s)	2347
Cac8I	GCNNGC	8 cut(s)	20, 342, 830, 1597, 1903, 2500, 2504, 3080
CaiI	CAGNNNCTG	2 cut(s)	244, 655
CciI	TCATGA	2 cut(s)	975, 1443
CfoI	GCGC	3 cut(s)	266, 2334, 3063
Cfr10I	RCCGGY	2 cut(s)	80, 685
Cfr13I	GGNCC	7 cut(s)	24, 83, 574, 631, 779, 2534, 3042
Cfr9I	CCCGGG	1 cut(s)	27
ClaI	ATCGAT	2 cut(s)	185, 195
Csp6I	GTAC	5 cut(s)	241, 739, 1505, 2689, 2866
CspAI	ACCGGT	1 cut(s)	80
CviQI	GTAC	5 cut(s)	241, 739, 1505, 2689, 2866
DraI	TTTAAA	2 cut(s)	1495, 1741
DrdI	GACNNNNNNGTC	1 cut(s)	2924
DseDI	GACNNNNNNGTC	1 cut(s)	2924
EaeI	YGGCCR	2 cut(s)	1899, 2496
Eam1104I	CTCTTC	2 cut(s)	2190, 2375
EarI	CTCTTC	2 cut(s)	2190, 2375
Ecl136II	GAGCTC	1 cut(s)	3087
Eco130I	CCWWGG	5 cut(s)	1809, 1848, 2787, 2808, 3090
Eco24I	GRGCYC	3 cut(s)	664, 1096, 3089
Eco47I	GGWCC	5 cut(s)	24, 83, 574, 779, 3042
Eco53kI	GAGCTC	1 cut(s)	3087
Eco57I	CTGAAG	3 cut(s)	1023, 1305, 1545
Eco81I	CCTNAGG	1 cut(s)	2726
Eco88I	CYCGRG	2 cut(s)	27, 102
EcoICRI	GAGCTC	1 cut(s)	3087
EcoNI	CCTNNNNNAGG	1 cut(s)	1604
EcoO109I	RGGNCCY	3 cut(s)	779, 2534, 3042
EcoRI	GAATTC	3 cut(s)	513, 2579, 2846
EcoRII	CCWGG	1 cut(s)	526
EcoT14I	CCWWGG	5 cut(s)	1809, 1848, 2787, 2808, 3090
EcoT22I	ATGCAT	1 cut(s)	1279
EcoT38I	GRGCYC	3 cut(s)	664, 1096, 3089
ErhI	CCWWGG	5 cut(s)	1809, 1848, 2787, 2808, 3090
FalI	AAGNNNNNCTT	2 cut(s)	2811, 2843
FaqI	GGGAC	4 cut(s)	10, 2141, 2846, 3028
FauI	CCCGC	2 cut(s)	226, 3056
FbaI	TGATCA	3 cut(s)	808, 1071, 2235
FblI	GTMKAC	3 cut(s)	931, 2613, 2916
Fnu4HI	GCNGC	9 cut(s)	129, 211, 222, 650, 902, 2664, 2712, 2715, 2796
FokI	GGATG	6 cut(s)	798, 1865, 1880, 1978, 2273, 2690
FriOI	GRGCYC	3 cut(s)	664, 1096, 3089
Fsp4HI	GCNGC	9 cut(s)	129, 211, 222, 650, 902, 2664, 2712, 2715, 2796
FspBI	CTAG	7 cut(s)	768, 1314, 1718, 1761, 2160, 2774, 3114
GlaI	GCGC	3 cut(s)	265, 2333, 3062
GluI	GCNGC	9 cut(s)	129, 211, 222, 650, 902, 2664, 2712, 2715, 2796
HaeIII	GGCC	7 cut(s)	632, 996, 1560, 1901, 2498, 2535, 2807
HapII	CCGG	7 cut(s)	28, 81, 207, 686, 1374, 1834, 3002
HhaI	GCGC	3 cut(s)	266, 2334, 3063
Hin6I	GCGC	3 cut(s)	264, 2332, 3061
HinP1I	GCGC	3 cut(s)	264, 2332, 3061
HincII	GTYRAC	3 cut(s)	868, 1387, 2065
HindII	GTYRAC	3 cut(s)	868, 1387, 2065
HindIII	AAGCTT	3 cut(s)	350, 918, 1634
HpaII	CCGG	7 cut(s)	28, 81, 207, 686, 1374, 1834, 3002
HphI	GGTGA	7 cut(s)	541, 704, 1081, 1346, 1528, 2651, 2750
Hpy99I	CGWCG	1 cut(s)	275
HpyAV	CCTTC	7 cut(s)	145, 580, 1600, 2003, 2197, 2872, 2907
HpyCH4III	ACNGT	6 cut(s)	973, 1238, 1244, 1672, 2094, 2914
HpyCH4IV	ACGT	2 cut(s)	1389, 2424
HpyF10VI	GCNNNNNNNGC	7 cut(s)	445, 1174, 1274, 1633, 2792, 2804, 3084
HpySE526I	ACGT	2 cut(s)	1389, 2424
HspAI	GCGC	3 cut(s)	264, 2332, 3061
Kpn2I	TCCGGA	1 cut(s)	1373
Ksp22I	TGATCA	3 cut(s)	808, 1071, 2235
LmnI	GCTCC	4 cut(s)	601, 2329, 2467, 3092
Lsp1109I	GCAGC	8 cut(s)	208, 222, 661, 913, 2650, 2698, 2726, 2782
LweI	GCATC	6 cut(s)	1177, 1286, 1795, 1956, 2256, 2947
MaeI	CTAG	7 cut(s)	768, 1314, 1718, 1761, 2160, 2774, 3114
MaeII	ACGT	2 cut(s)	1389, 2424
MaeIII	GTNAC	4 cut(s)	254, 699, 1238, 1291
MfeI	CAATTG	2 cut(s)	1577, 2594
MflI	RGATCY	3 cut(s)	1108, 1663, 2881
MhlI	GDGCHC	9 cut(s)	524, 664, 802, 1096, 1461, 1687, 2016, 3080, 3089
MlsI	TGGCCA	2 cut(s)	1901, 2498
MluNI	TGGCCA	2 cut(s)	1901, 2498
MlyI	GAGTC	4 cut(s)	86, 114, 548, 1625
MmeI	TCCRAC	4 cut(s)	18, 297, 1691, 1845
Mox20I	TGGCCA	2 cut(s)	1901, 2498
Mph1103I	ATGCAT	1 cut(s)	1279
MroI	TCCGGA	1 cut(s)	1373
MroXI	GAANNNNTTC	3 cut(s)	186, 1008, 2823
MscI	TGGCCA	2 cut(s)	1901, 2498
MslI	CAYNNNNRTG	4 cut(s)	795, 1889, 2169, 2873
Msp20I	TGGCCA	2 cut(s)	1901, 2498
MspA1I	CMGCKG	5 cut(s)	22, 221, 479, 938, 2663
MspCI	CTTAAG	2 cut(s)	620, 2600
MspI	CCGG	7 cut(s)	28, 81, 207, 686, 1374, 1834, 3002
MspR9I	CCNGG	4 cut(s)	28, 29, 528, 1834
MunI	CAATTG	2 cut(s)	1577, 2594
Mva1269I	GAATGC	2 cut(s)	436, 1439
MvaI	CCWGG	1 cut(s)	528
MvnI	CGCG	2 cut(s)	264, 3063
MwoI	GCNNNNNNNGC	7 cut(s)	445, 1174, 1274, 1633, 2792, 2804, 3084
NciI	CCSGG	3 cut(s)	28, 29, 1834
NcoI	CCATGG	2 cut(s)	1809, 2808
NlaIV	GGNNCC	8 cut(s)	26, 576, 780, 1665, 1922, 2015, 2883, 3044
NmuCI	GTSAC	1 cut(s)	699
NsiI	ATGCAT	1 cut(s)	1279
NspI	RCATGY	5 cut(s)	1138, 1277, 2156, 2506, 2995
NspV	TTCGAA	1 cut(s)	419
PaeI	GCATGC	1 cut(s)	2506
PagI	TCATGA	2 cut(s)	975, 1443
PcsI	WCGNNNNNNNCGW	1 cut(s)	425
PctI	GAATGC	2 cut(s)	436, 1439
PdmI	GAANNNNTTC	3 cut(s)	186, 1008, 2823
PflFI	GACNNNGTC	2 cut(s)	47, 872
PfoI	TCCNGGA	1 cut(s)	1832
PinAI	ACCGGT	1 cut(s)	80
PkrI	GCNGC	9 cut(s)	130, 212, 223, 651, 903, 2665, 2713, 2716, 2797
Ple19I	CGATCG	1 cut(s)	199
PleI	GAGTC	4 cut(s)	86, 113, 548, 1624
PpsI	GAGTC	4 cut(s)	86, 113, 548, 1624
PpuMI	RGGWCCY	2 cut(s)	779, 3042
PshBI	ATTAAT	1 cut(s)	1305
PsiI	TTATAA	1 cut(s)	1889
Psp124BI	GAGCTC	1 cut(s)	3089
Psp5II	RGGWCCY	2 cut(s)	779, 3042
Psp6I	CCWGG	1 cut(s)	526
PspGI	CCWGG	1 cut(s)	526
PspN4I	GGNNCC	8 cut(s)	26, 576, 780, 1665, 1922, 2015, 2883, 3044
PspPI	GGNCC	7 cut(s)	24, 83, 574, 631, 779, 2534, 3042
PspPPI	RGGWCCY	2 cut(s)	779, 3042
PstI	CTGCAG	2 cut(s)	2323, 2716
PstNI	CAGNNNCTG	2 cut(s)	244, 655
PsuI	RGATCY	3 cut(s)	1108, 1663, 2881
PsyI	GACNNNGTC	2 cut(s)	47, 872
PvuI	CGATCG	1 cut(s)	199
PvuII	CAGCTG	3 cut(s)	479, 938, 2663
RsaI	GTAC	5 cut(s)	242, 740, 1506, 2690, 2867
RsaNI	GTAC	5 cut(s)	241, 739, 1505, 2689, 2866
RseI	CAYNNNNRTG	4 cut(s)	795, 1889, 2169, 2873
SacI	GAGCTC	1 cut(s)	3089
SatI	GCNGC	9 cut(s)	129, 211, 222, 650, 902, 2664, 2712, 2715, 2796
Sau96I	GGNCC	7 cut(s)	24, 83, 574, 631, 779, 2534, 3042
SchI	GAGTC	4 cut(s)	86, 114, 548, 1625
ScrFI	CCNGG	4 cut(s)	28, 29, 528, 1834
SduI	GDGCHC	9 cut(s)	524, 664, 802, 1096, 1461, 1687, 2016, 3080, 3089
SfaNI	GCATC	6 cut(s)	1177, 1286, 1795, 1956, 2256, 2947
SfcI	CTRYAG	4 cut(s)	1234, 2319, 2445, 2712
SfuI	TTCGAA	1 cut(s)	419
SinI	GGWCC	5 cut(s)	24, 83, 574, 779, 3042
SmaI	CCCGGG	1 cut(s)	29
SmiMI	CAYNNNNRTG	4 cut(s)	795, 1889, 2169, 2873
SmlI	CTYRAG	9 cut(s)	620, 1052, 1356, 1601, 2027, 2343, 2379, 2600, 3073
SmoI	CTYRAG	9 cut(s)	620, 1052, 1356, 1601, 2027, 2343, 2379, 2600, 3073
SpeI	ACTAGT	1 cut(s)	3113
SphI	GCATGC	1 cut(s)	2506
SsiI	CCGC	8 cut(s)	22, 55, 128, 219, 471, 1980, 2987, 3063
SspI	AATATT	4 cut(s)	1418, 2113, 2125, 2389
SspMI	CTAG	7 cut(s)	768, 1314, 1718, 1761, 2160, 2774, 3114
SstI	GAGCTC	1 cut(s)	3089
StyD4I	CCNGG	4 cut(s)	26, 27, 526, 1832
StyI	CCWWGG	5 cut(s)	1809, 1848, 2787, 2808, 3090
TaaI	ACNGT	6 cut(s)	973, 1238, 1244, 1672, 2094, 2914
TaiI	ACGT	2 cut(s)	1392, 2427
TaqI	TCGA	9 cut(s)	185, 195, 414, 419, 564, 2070, 2167, 2853, 3053
TaqII	GACCGA	1 cut(s)	2934
TatI	WGTACW	3 cut(s)	738, 1504, 2865
TauI	GCSGC	1 cut(s)	131
TscAI	CASTG	7 cut(s)	524, 660, 802, 911, 1156, 2083, 2323
TseFI	GTSAC	1 cut(s)	699
TseI	GCWGC	8 cut(s)	210, 221, 649, 901, 2663, 2711, 2714, 2795
Tsp45I	GTSAC	1 cut(s)	699
TspGWI	ACGGA	4 cut(s)	319, 1784, 2573, 2952
TspMI	CCCGGG	1 cut(s)	27
TspRI	CASTG	7 cut(s)	524, 660, 802, 911, 1156, 2083, 2323
Tth111I	GACNNNGTC	2 cut(s)	47, 872
Vha464I	CTTAAG	2 cut(s)	620, 2600
VpaK11BI	GGWCC	5 cut(s)	24, 83, 574, 779, 3042
VspI	ATTAAT	1 cut(s)	1305
XagI	CCTNNNNNAGG	1 cut(s)	1604
XapI	RAATTY	7 cut(s)	409, 513, 1192, 2218, 2277, 2579, 2846
XceI	RCATGY	5 cut(s)	1138, 1277, 2156, 2506, 2995
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XmaI	CCCGGG	1 cut(s)	27
XmiI	GTMKAC	3 cut(s)	931, 2613, 2916
XmnI	GAANNNNTTC	3 cut(s)	186, 1008, 2823
XspI	CTAG	7 cut(s)	768, 1314, 1718, 1761, 2160, 2774, 3114
Zsp2I	ATGCAT	1 cut(s)	1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.