Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0004171

Physical Location & Seq

Genomic Coordinates Forward (+)

82343293 .. 82349680

Gene Structure 6388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG602800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGTCGACCCCAAAACCGCAGAGGTCTAAAGAAGAGCTGGAGGACATAATCCTCCGGAGAATCTTCCAGGTCTCGCTGACGGAGCCGGCCGAGCCCAATTCGCGAGTCGTCTACTTGGAGATGACGGCGGCGGAGATTCTCAGCGAGGGCAAGCCGATGAGGCTCACGCGCGACCTCATGGAGTCGATCCTGATCGACCGTTTAATGGGTAGCTTCGCCGCCGTGGAGCCGCCGTTTCAGTACCTTGTCGGGTGTTACCGACGCGCCTACGACGAGGGTCGGAAAATCACCGTCATGAAAGATAAGAACTTGAGGGCGGATATGGAGTCGGTGATTAAGCAGGCGAAGAAGCTTTCGGTTTCGTATTGTAGGATCCATTTGGGGAACCCGGAGGCGTTTCCGGGTTCGGGTTCGGATCCGGCGACGGCGTCAAATGCGTCGCCGTTGCTGCCGTTGGTTTTTTCCGAGGTGGGGAGCGGCTCGGTGGATGAGTTTGGAGGGAGTTCTAGTACTAGTAGTGGAGTTCAGTGCCCACCTGGGTTTTTGGATGACTTCTTTAGGGACTCGGATTTCGATAGCTTGGACCCAATTTTGAAGGGTTTGTATGAGGAGCTTAGGGAGATTGTGCTCAAGGTTTCGGCATTGGGGAATTTCCAGCAGCCATTGAGGGCTCTGTTGTATTTGGTTAGCTTTCCTGCTGGGGCTAAGAGTCTGGTGAATCACCCGTGGTGGATTCCCAAAGGTGTTTACTTGAATGGGAGGGTTATCGAAAGGACTAGTATTTTGGGTCCTTTTTTTCATGTCAGTGCTCTGCCGGATCATCCTATTTTCAAGAGCCAGCCGGATGTGGGGCAGCAGTGCTTTTCAGAAAATTCTAAGGATAGTGATCTGCGATCTTCGTACGCCACAATAAAGACACTTACGAATAGTCTATATGACGGCCTCGCAGAAGTTCTCCTCAAACTATTAAAAAATGCAGAGACCCGTGAGAGTGTTCTTGAGTATCTTGCTGAGGTTATCAACAAAAACTCATCAAGGGCTCATATTCAGGTCGATCCTCTTTCTTGTGCAAGTTCTGGCATGTTTGTCAATCTCAGTGCTGTCATGCTTCGACTTTGCGAGCCATTTCTGGATCCAAATTTAACAAAAAGGGACAAAATTGATCCAAAATATGTATTCTATGGTGACCGTCTGGAGTTAAGAGGTTTGACTGCTCTACATGCATCATCTGAAGAAGTTACTGAATGGATTAACAAAGATAGTCTCGGGAATGCTGGTGGTGATGGTCAAAATCGCCTTTCACAATCCCAAGAAGCCACCAGCTCTGGCAGTAATATATCTCAACCTTCTAACATGAAGCCAAGCGGCGAAAAATCTAAATCATTTATTTGTGAATGCTTCTTCATGACTGCGAGGGTGCTCAACCTGGGTTTATTGAAGGCACTTTCTGACTATAAGCATCTAGTACAGGACATGCAGAGGTCTGAAGATACACTTAAAGCCTTGAAAAGACAGGCACCTTCTCCTCAATTGAATATTGATATAGCTCGCCTTGAGAAAGAGTTAGAGTTGTATAAACAGGAAAAGCGTTGTCATGAAGCTCAGATACTCACGGATGAACCACTTTTAGAGCGTGCTCTTTCTTTCTACCGGTTGATGGTGGTGTGGCTAGTTCGCTTGGTTGGTGGATTTAAAATGCCTTTGCCGTCAACTTGCCCCGTGGAATTTGCATCTATGCCAGAACATTTTGTGGAAGATGCCATGGAGTTGCTTATATTCGCTTCCCGGATGCCAAAAGCATTTCATGGGATCCTGTTGGTACGTTCTTATCATAAAGCAATTTACCAAATTTTGTTCTTATTTTCGCTAGTCTCATTTTCATATGCTGTTTTATTTGACGTGTTATATATTTTTATTTTGCAGGATGAATTTATGAACTTTATTATAATGTTCATGGCAAGTCCAGAATATATTAGGAACCCTTATCTAAGAGCAAAGATGGTTGAAGTCCTGAATTGCTTGATGCCCCGTCCAAGTCATCAATCACCTGCTGCTACAGCTGCTGCTGCTAGCCTGTTCGAAGGGCACCAACTGTCTCTCGAGTATCTTGTGAGGAATCTCTTGAAACTATATGTAGACATTGAGTTCACTGGTTCTCATACACAGTTCTATGACAAGTTCAACATCCGTCACAATATTGCTGAACTTCTTGAATATCTTTGGCAAGTCCCCAGTCATCAAAATGCTTGGAAACAGATTGCTAGGGAAGAAGAGAAAGGCGTATATTTGAATTTCTTAAACTTCTTGATCAATGATAGTATATATCTTCTTGATGAAAGTCTTAACAAGATTCTTGAACACAAAGAACTGGAGGCTGAGATGTCAAACACCGCAGAATGGGAGCGTAGACCCGCTCAAGAGAGGCAGGAGAGAACCCGCCTGTTCCACTCTCAAGAAAATATTATCCGAATTGATATGAAGTTAGCAAATGAAGATGTGAGTATGTTGGCATTTACTACGGAACAAATTACAGCTCCTTTCCTACTTCCTGAGATGGTTGAAAGGGTGGCAAGTATGCTCAATTATTTTTTGTTGCAATTAGTAGGTCCTCAAAGAAAATCTCTTAGTCTGAAAGACCCGGAGAAGTATGAGTTCCGCCCAAAGCAATTGCTTAAGCAGATTGTGTACATATATGTTAATCTGGCAAGAGGTGATACGGAAAATATCTTTCCAGCTGCTATCTCAAAGGATGGTCGATCTTACAATGAGCAGTTGTTCAGTGCTGCTGCCGATGTCCTAAGGAGAATTGGTGAAGATGGAAGGGTCATACAGGAGTTTGTTGAGCTTGGTTCCAAAGCCAAGGTTGCTGCTTCTGAGGCCATGGACGCTGAAGCTGTTCTTGGGGATATACCAGATGAATTCCTTGACCCAATTCAGTACACTTTAATGAAGGATCCAGTTATCTTGCCTTCTTCAAGAATCACAGTAGACCGGCCTGTCATTCAAAGGCATCTTCTTAGTGATAATAGTGATCCATTCAACCGTTCCCATCTTACTGCGGACATGTTGATACCGGATAATGATTTGAAGGCAAAAATACAAGAGTTTATTAGGTCGCAACAGTCGAAGCGCGGGGAAAGCCTTAGCATGCAGAGTGATAAGGCAACAATACAAACCACATCTAGTGAAATGCTAATTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0030433 GO:0032446 GO:0033554 GO:0034450 GO:0034976 GO:0036211 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04120 ko04141 map04120 map04141

Pfam Domains

Protein Families

U-box Ufd2P_core

Protein Analysis

1067

Amino Acids

120.97

Weight (kDa)

5.53

Isoelectric Point (pI)

51.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	262 - 953	9.7e-196	Ubiquitin elongating factor core
U-box	PF04564	970 - 1041	1.1e-29	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15400
fragaria_vesca	FvH4_3g25920 FvH4_3g25930 FvH4_6g47550 FvH4_6g47550 FvH4_6g47550
malus_domestica	MD09G1060800.v1.1 MD17G1056200.v1.1
prunus_persica	Prupe.3G259400_v2.0.a1
pyrus_communis	pycom111g05110 pycom17g05530
rosa_chinensis	RchiOBHm_Chr2g0166651 RchiOBHm_Chr5g0047221
rosa_laevigata	RLG00000021638 RLG00000034480
rosa_multiflora	Rmu_sc0002310.1_g000029 Rmu_sc0005232.1_g000002
rosa_roxburghii	Rroxscaffold_1G00034160 Rroxscaffold_2G00084330 Rroxscaffold_2G00084640
rosa_rugosa	Rorug02G0524700 Rorug05G0235100
rosa_samantha	Rh2AG590900 Rh2BG602800 Rh2CG573700 Rh2DG613600 Rh5AG314000 Rh5BG322500 Rh5CG349700 Rh5CG349800 Rh5DG333500
rosa_wichuraiana	Rw2G049220 Rw5G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1946
AarI	CACCTGC	1 cut(s)	2056
AasI	GACNNNNNNGTC	1 cut(s)	2996
Acc36I	ACCTGC	1 cut(s)	2056
AccB1I	GGYRCC	2 cut(s)	1516, 2085
AccBSI	CCGCTC	2 cut(s)	477, 2414
AccI	GTMKAC	5 cut(s)	5, 111, 2136, 2407, 2988
AccII	CGCG	5 cut(s)	103, 169, 171, 264, 3132
AccIII	TCCGGA	1 cut(s)	54
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	8 cut(s)	649, 871, 1138, 1724, 1848, 1928, 2290, 2918
AcuI	CTGAAG	3 cut(s)	1251, 1506, 2910
AcyI	GRCGYC	1 cut(s)	428
AfaI	GTAC	7 cut(s)	242, 511, 902, 1467, 1821, 2687, 2939
AfiI	CCNNNNNNNGG	6 cut(s)	205, 407, 471, 848, 1657, 2397
AflII	CTTAAG	1 cut(s)	2672
AflIII	ACRYGT	2 cut(s)	1899, 3063
AgeI	ACCGGT	1 cut(s)	1650
AhlI	ACTAGT	2 cut(s)	512, 776
AjiI	CACGTC	1 cut(s)	1900
AjnI	CCWGG	3 cut(s)	66, 535, 1425
AjuI	GAANNNNNNNTTGG	2 cut(s)	2883, 2915
Alw21I	GWGCWC	4 cut(s)	630, 811, 1422, 1639
Alw26I	GTCTC	5 cut(s)	76, 974, 1268, 1876, 2100
AlwNI	CAGNNNCTG	1 cut(s)	2063
Ama87I	CYCGRG	2 cut(s)	1265, 2099
Aor13HI	TCCGGA	1 cut(s)	54
AoxI	GGCC	4 cut(s)	87, 940, 2877, 2993
ApoI	RAATTY	8 cut(s)	649, 871, 1138, 1724, 1848, 1928, 2290, 2918
ArsI	GACNNNNNNTTYG	4 cut(s)	554, 586, 766, 798
AsiGI	ACCGGT	1 cut(s)	1650
Asp700I	GAANNNNTTC	1 cut(s)	2895
AspLEI	GCGC	3 cut(s)	171, 266, 3132
AspS9I	GGNCC	3 cut(s)	583, 788, 2606
AsuC2I	CCSGG	4 cut(s)	389, 402, 1786, 2639
AsuHPI	GGTGA	9 cut(s)	280, 343, 713, 727, 1196, 1292, 2037, 2723, 2822
AsuII	TTCGAA	1 cut(s)	2079
AsuNHI	GCTAGC	1 cut(s)	2069
AvaI	CYCGRG	2 cut(s)	1265, 2099
AvaII	GGWCC	3 cut(s)	583, 788, 2606
AxyI	CCTNAGG	1 cut(s)	2798
BaeGI	GKGCMC	2 cut(s)	533, 2088
BamHI	GGATCC	5 cut(s)	372, 415, 1132, 1809, 2953
BanI	GGYRCC	2 cut(s)	1516, 2085
BanII	GRGCYC	3 cut(s)	96, 673, 1042
BarI	GAAGNNNNNNTAC	2 cut(s)	1590, 1622
Bbv12I	GWGCWC	4 cut(s)	630, 811, 1422, 1639
BbvCI	CCTCAGC	1 cut(s)	1011
BccI	CCATC	7 cut(s)	1277, 1651, 1993, 2548, 2744, 2810, 3057
BceAI	ACGGC	8 cut(s)	141, 206, 217, 427, 436, 441, 955, 1690
BciT130I	CCWGG	3 cut(s)	68, 537, 1427
BclI	TGATCA	1 cut(s)	2307
BcnI	CCSGG	4 cut(s)	389, 402, 1786, 2639
BcoDI	GTCTC	5 cut(s)	76, 974, 1268, 1876, 2100
BcuI	ACTAGT	2 cut(s)	512, 776
BfaI	CTAG	9 cut(s)	507, 513, 777, 1463, 1670, 1868, 2070, 2262, 3183
BfmI	CTRYAG	1 cut(s)	2055
BfrI	CTTAAG	1 cut(s)	2672
BfuAI	ACCTGC	1 cut(s)	2056
BglI	GCCNNNNNGGC	1 cut(s)	160
BmcAI	AGTACT	1 cut(s)	511
Bme1390I	CCNGG	7 cut(s)	68, 389, 402, 537, 1427, 1786, 2639
Bme18I	GGWCC	3 cut(s)	583, 788, 2606
BmeT110I	CYCGRG	2 cut(s)	1265, 2099
BmgBI	CACGTC	1 cut(s)	1900
BmgT120I	GGNCC	3 cut(s)	583, 788, 2606
BmrFI	CCNGG	7 cut(s)	68, 389, 402, 537, 1427, 1786, 2639
BmrI	ACTGGG	1 cut(s)	2226
BmsI	GCATC	7 cut(s)	1232, 1468, 1739, 1747, 1779, 2013, 3019
BmtI	GCTAGC	1 cut(s)	2073
BmuI	ACTGGG	1 cut(s)	2226
BoxI	GACNNNNGTC	1 cut(s)	276
BpmI	CTGGAG	3 cut(s)	59, 1214, 2390
Bpu10I	CCTNAGC	3 cut(s)	614, 1011, 3143
Bpu14I	TTCGAA	1 cut(s)	2079
BpuEI	CTTGAG	6 cut(s)	331, 614, 1019, 1574, 2400, 2436
BpuMI	CCSGG	4 cut(s)	389, 402, 1786, 2639
BsaBI	GATNNNNATC	2 cut(s)	191, 885
BsaHI	GRCGYC	1 cut(s)	428
BsaI	GGTCTC	2 cut(s)	76, 974
BsaJI	CCNNGG	9 cut(s)	222, 465, 536, 725, 1426, 1719, 1761, 2859, 2880
BsaWI	WCCGGW	3 cut(s)	54, 1650, 3073
BsaXI	ACNNNNNCTCC	6 cut(s)	218, 248, 493, 523, 2794, 2824
Bsc4I	CCNNNNNNNGG	6 cut(s)	205, 407, 471, 848, 1657, 2397
Bse118I	RCCGGY	3 cut(s)	85, 1650, 2991
Bse1I	ACTGG	4 cut(s)	2155, 2232, 2373, 2957
Bse21I	CCTNAGG	1 cut(s)	2798
Bse8I	GATNNNNATC	2 cut(s)	191, 885
BseAI	TCCGGA	1 cut(s)	54
BseBI	CCWGG	3 cut(s)	68, 537, 1427
BseDI	CCNNGG	9 cut(s)	222, 465, 536, 725, 1426, 1719, 1761, 2859, 2880
BseGI	GGATG	9 cut(s)	493, 553, 820, 850, 1621, 1794, 1930, 2184, 2755
BseJI	GATNNNNATC	2 cut(s)	191, 885
BseLI	CCNNNNNNNGG	6 cut(s)	205, 407, 471, 848, 1657, 2397
BseMII	CTCAG	7 cut(s)	154, 1002, 1108, 1616, 2367, 2541, 2865
BseNI	ACTGG	4 cut(s)	2155, 2232, 2373, 2957
BseRI	GAGGAG	3 cut(s)	623, 947, 1515
BseSI	GKGCMC	2 cut(s)	533, 2088
BseX3I	CGGCCG	1 cut(s)	87
BseYI	CCCAGC	1 cut(s)	698
Bsh1236I	CGCG	5 cut(s)	103, 169, 171, 264, 3132
Bsh1285I	CGRYCG	2 cut(s)	90, 199
BshFI	GGCC	4 cut(s)	89, 942, 2879, 2995
BshNI	GGYRCC	2 cut(s)	1516, 2085
BshTI	ACCGGT	1 cut(s)	1650
BsiEI	CGRYCG	2 cut(s)	90, 199
BsiHKAI	GWGCWC	4 cut(s)	630, 811, 1422, 1639
BsiHKCI	CYCGRG	2 cut(s)	1265, 2099
BsiWI	CGTACG	1 cut(s)	900
BslFI	GGGAC	3 cut(s)	575, 1166, 2213
BslI	CCNNNNNNNGG	6 cut(s)	205, 407, 471, 848, 1657, 2397
BsmAI	GTCTC	5 cut(s)	76, 974, 1268, 1876, 2100
BsmFI	GGGAC	3 cut(s)	575, 1166, 2213
BsmI	GAATGC	2 cut(s)	1276, 1400
BsnI	GGCC	4 cut(s)	89, 942, 2879, 2995
Bso31I	GGTCTC	2 cut(s)	76, 974
BsoBI	CYCGRG	2 cut(s)	1265, 2099
Bsp119I	TTCGAA	1 cut(s)	2079
Bsp1286I	GDGCHC	9 cut(s)	96, 533, 630, 673, 811, 1042, 1422, 1639, 2088
Bsp13I	TCCGGA	1 cut(s)	54
Bsp1407I	TGTACA	1 cut(s)	2685
Bsp19I	CCATGG	2 cut(s)	1761, 2880
Bsp68I	TCGCGA	1 cut(s)	103
BspANI	GGCC	4 cut(s)	89, 942, 2879, 2995
BspCNI	CTCAG	7 cut(s)	153, 1003, 1107, 1615, 2368, 2542, 2866
BspEI	TCCGGA	1 cut(s)	54
BspFNI	CGCG	5 cut(s)	103, 169, 171, 264, 3132
BspHI	TCATGA	3 cut(s)	294, 1404, 1594
BspMI	ACCTGC	1 cut(s)	2056
BspOI	GCTAGC	1 cut(s)	2073
BspQI	GCTCTTC	1 cut(s)	27
BspT104I	TTCGAA	1 cut(s)	2079
BspT107I	GGYRCC	2 cut(s)	1516, 2085
BspTI	CTTAAG	1 cut(s)	2672
BspTNI	GGTCTC	2 cut(s)	76, 974
BsrBI	CCGCTC	2 cut(s)	477, 2414
BsrFI	RCCGGY	3 cut(s)	85, 1650, 2991
BsrGI	TGTACA	1 cut(s)	2685
BsrI	ACTGG	4 cut(s)	2155, 2232, 2373, 2957
BssAI	RCCGGY	3 cut(s)	85, 1650, 2991
BssECI	CCNNGG	9 cut(s)	222, 465, 536, 725, 1426, 1719, 1761, 2859, 2880
BssNI	GRCGYC	1 cut(s)	428
BssT1I	CCWWGG	3 cut(s)	1761, 2859, 2880
Bst2UI	CCWGG	3 cut(s)	68, 537, 1427
Bst4CI	ACNGT	8 cut(s)	200, 292, 1190, 2094, 2166, 2986, 3044, 3123
Bst6I	CTCTTC	2 cut(s)	27, 2265
BstACI	GRCGYC	1 cut(s)	428
BstAFI	CTTAAG	1 cut(s)	2672
BstAUI	TGTACA	1 cut(s)	2685
BstBI	TTCGAA	1 cut(s)	2079
BstC8I	GCNNGC	9 cut(s)	87, 152, 342, 839, 1121, 1549, 1635, 2071, 3149
BstDSI	CCRYGG	5 cut(s)	222, 725, 1719, 1761, 2880
BstEII	GGTNACC	1 cut(s)	1184
BstF5I	GGATG	9 cut(s)	493, 553, 820, 850, 1621, 1794, 1930, 2184, 2755
BstFNI	CGCG	5 cut(s)	103, 169, 171, 264, 3132
BstHHI	GCGC	3 cut(s)	171, 266, 3132
BstMAI	GTCTC	5 cut(s)	76, 974, 1268, 1876, 2100
BstMCI	CGRYCG	2 cut(s)	90, 199
BstNI	CCWGG	3 cut(s)	68, 537, 1427
BstNSI	RCATGY	5 cut(s)	1084, 1223, 1477, 3067, 3151
BstPAI	GACNNNNGTC	1 cut(s)	276
BstPI	GGTNACC	1 cut(s)	1184
BstSCI	CCNGG	7 cut(s)	66, 387, 400, 535, 1425, 1784, 2637
BstSFI	CTRYAG	1 cut(s)	2055
BstSLI	GKGCMC	2 cut(s)	533, 2088
BstUI	CGCG	5 cut(s)	103, 169, 171, 264, 3132
BstX2I	RGATCY	5 cut(s)	372, 415, 1132, 1809, 2953
BstYI	RGATCY	5 cut(s)	372, 415, 1132, 1809, 2953
BstZI	CGGCCG	1 cut(s)	87
Bsu36I	CCTNAGG	1 cut(s)	2798
BsuRI	GGCC	4 cut(s)	89, 942, 2879, 2995
BtgI	CCRYGG	5 cut(s)	222, 725, 1719, 1761, 2880
BtrI	CACGTC	1 cut(s)	1900
BtsCI	GGATG	9 cut(s)	493, 553, 820, 850, 1621, 1794, 1930, 2184, 2755
BtsI	GCAGTG	1 cut(s)	863
BtsIMutI	CAGTG	6 cut(s)	533, 811, 863, 1102, 2148, 2785
BtuMI	TCGCGA	1 cut(s)	103
BveI	ACCTGC	1 cut(s)	2056
Cac8I	GCNNGC	9 cut(s)	87, 152, 342, 839, 1121, 1549, 1635, 2071, 3149
CaiI	CAGNNNCTG	1 cut(s)	2063
CciI	TCATGA	3 cut(s)	294, 1404, 1594
CfoI	GCGC	3 cut(s)	171, 266, 3132
Cfr10I	RCCGGY	3 cut(s)	85, 1650, 2991
Cfr13I	GGNCC	3 cut(s)	583, 788, 2606
CseI	GACGC	4 cut(s)	270, 417, 426, 2894
Csp6I	GTAC	7 cut(s)	241, 510, 901, 1466, 1820, 2686, 2938
CspAI	ACCGGT	1 cut(s)	1650
CviQI	GTAC	7 cut(s)	241, 510, 901, 1466, 1820, 2686, 2938
DraI	TTTAAA	1 cut(s)	1693
DrdI	GACNNNNNNGTC	1 cut(s)	2996
DseDI	GACNNNNNNGTC	1 cut(s)	2996
EaeI	YGGCCR	1 cut(s)	87
EagI	CGGCCG	1 cut(s)	87
Eam1104I	CTCTTC	2 cut(s)	27, 2265
EarI	CTCTTC	2 cut(s)	27, 2265
EciI	GGCGGA	3 cut(s)	146, 332, 2645
EclXI	CGGCCG	1 cut(s)	87
Eco130I	CCWWGG	3 cut(s)	1761, 2859, 2880
Eco24I	GRGCYC	3 cut(s)	96, 673, 1042
Eco31I	GGTCTC	2 cut(s)	76, 974
Eco47I	GGWCC	3 cut(s)	583, 788, 2606
Eco52I	CGGCCG	1 cut(s)	87
Eco57I	CTGAAG	3 cut(s)	1251, 1506, 2910
Eco81I	CCTNAGG	1 cut(s)	2798
Eco88I	CYCGRG	2 cut(s)	1265, 2099
Eco91I	GGTNACC	1 cut(s)	1184
EcoO109I	RGGNCCY	2 cut(s)	788, 2606
EcoO65I	GGTNACC	1 cut(s)	1184
EcoRI	GAATTC	1 cut(s)	2918
EcoRII	CCWGG	3 cut(s)	66, 535, 1425
EcoT14I	CCWWGG	3 cut(s)	1761, 2859, 2880
EcoT22I	ATGCAT	1 cut(s)	1225
EcoT38I	GRGCYC	3 cut(s)	96, 673, 1042
ErhI	CCWWGG	3 cut(s)	1761, 2859, 2880
FalI	AAGNNNNNCTT	4 cut(s)	1479, 1511, 2883, 2915
FaqI	GGGAC	3 cut(s)	575, 1166, 2213
FauI	CCCGC	3 cut(s)	2419, 2444, 3125
FauNDI	CATATG	1 cut(s)	1882
FbaI	TGATCA	1 cut(s)	2307
FblI	GTMKAC	5 cut(s)	5, 111, 2136, 2407, 2988
FokI	GGATG	9 cut(s)	500, 560, 807, 857, 1628, 1801, 1937, 2171, 2762
FriOI	GRGCYC	3 cut(s)	96, 673, 1042
FspBI	CTAG	9 cut(s)	507, 513, 777, 1463, 1670, 1868, 2070, 2262, 3183
GlaI	GCGC	3 cut(s)	170, 265, 3131
GsaI	CCCAGC	1 cut(s)	702
GsuI	CTGGAG	3 cut(s)	59, 1214, 2390
HaeIII	GGCC	4 cut(s)	89, 942, 2879, 2995
HgaI	GACGC	4 cut(s)	270, 417, 426, 2894
HhaI	GCGC	3 cut(s)	171, 266, 3132
Hin1I	GRCGYC	1 cut(s)	428
Hin6I	GCGC	3 cut(s)	169, 264, 3130
HinP1I	GCGC	3 cut(s)	169, 264, 3130
HincII	GTYRAC	2 cut(s)	6, 1710
HindII	GTYRAC	2 cut(s)	6, 1710
HindIII	AAGCTT	1 cut(s)	350
HphI	GGTGA	9 cut(s)	280, 343, 713, 727, 1196, 1292, 2037, 2723, 2822
Hpy99I	CGWCG	4 cut(s)	264, 275, 427, 442
HpyAV	CCTTC	9 cut(s)	589, 1356, 1432, 1530, 2075, 2814, 2944, 2979, 3082
HpyCH4III	ACNGT	8 cut(s)	200, 292, 1190, 2094, 2166, 2986, 3044, 3123
HpyCH4IV	ACGT	2 cut(s)	1822, 1899
HpyCH4V	TGCA	8 cut(s)	977, 1070, 1223, 1477, 1730, 1921, 2596, 3151
HpySE526I	ACGT	2 cut(s)	1822, 1899
Hsp92I	GRCGYC	1 cut(s)	428
HspAI	GCGC	3 cut(s)	169, 264, 3130
Kpn2I	TCCGGA	1 cut(s)	54
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	1 cut(s)	2307
LguI	GCTCTTC	1 cut(s)	27
LmnI	GCTCC	6 cut(s)	82, 226, 474, 610, 2401, 2539
LweI	GCATC	7 cut(s)	1232, 1468, 1739, 1747, 1779, 2013, 3019
MaeI	CTAG	9 cut(s)	507, 513, 777, 1463, 1670, 1868, 2070, 2262, 3183
MaeII	ACGT	2 cut(s)	1822, 1899
MaeIII	GTNAC	4 cut(s)	254, 1184, 1237, 2189
MbiI	CCGCTC	2 cut(s)	477, 2414
MfeI	CAATTG	2 cut(s)	1529, 2666
MflI	RGATCY	5 cut(s)	372, 415, 1132, 1809, 2953
MhlI	GDGCHC	9 cut(s)	96, 533, 630, 673, 811, 1042, 1422, 1639, 2088
MlyI	GAGTC	5 cut(s)	114, 191, 335, 557, 718
MmeI	TCCRAC	1 cut(s)	260
Mph1103I	ATGCAT	1 cut(s)	1225
MroI	TCCGGA	1 cut(s)	54
MroNI	GCCGGC	1 cut(s)	85
MroXI	GAANNNNTTC	1 cut(s)	2895
MslI	CAYNNNNRTG	4 cut(s)	293, 804, 2241, 2945
MspA1I	CMGCKG	2 cut(s)	2060, 2735
MspCI	CTTAAG	1 cut(s)	2672
MspR9I	CCNGG	7 cut(s)	68, 389, 402, 537, 1427, 1786, 2639
MunI	CAATTG	2 cut(s)	1529, 2666
Mva1269I	GAATGC	2 cut(s)	1276, 1400
MvaI	CCWGG	3 cut(s)	68, 537, 1427
MvnI	CGCG	5 cut(s)	103, 169, 171, 264, 3132
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	4 cut(s)	389, 402, 1786, 2639
NcoI	CCATGG	2 cut(s)	1761, 2880
NdeI	CATATG	1 cut(s)	1882
NgoMIV	GCCGGC	1 cut(s)	85
NheI	GCTAGC	1 cut(s)	2069
NmeAIII	GCCGAG	1 cut(s)	115
NmuCI	GTSAC	2 cut(s)	1184, 2189
NruI	TCGCGA	1 cut(s)	103
NsiI	ATGCAT	1 cut(s)	1225
NspI	RCATGY	5 cut(s)	1084, 1223, 1477, 3067, 3151
NspV	TTCGAA	1 cut(s)	2079
PaeI	GCATGC	1 cut(s)	3151
PaeR7I	CTCGAG	1 cut(s)	2099
PagI	TCATGA	3 cut(s)	294, 1404, 1594
PaqCI	CACCTGC	1 cut(s)	2056
PciI	ACATGT	1 cut(s)	3063
PciSI	GCTCTTC	1 cut(s)	27
PcsI	WCGNNNNNNNCGW	2 cut(s)	419, 3122
PctI	GAATGC	2 cut(s)	1276, 1400
PdiI	GCCGGC	1 cut(s)	87
PdmI	GAANNNNTTC	1 cut(s)	2895
PfeI	GAWTC	7 cut(s)	60, 136, 718, 733, 2116, 2350, 2979
Pfl23II	CGTACG	1 cut(s)	900
PflFI	GACNNNGTC	1 cut(s)	427
PfoI	TCCNGGA	1 cut(s)	1784
PinAI	ACCGGT	1 cut(s)	1650
PleI	GAGTC	5 cut(s)	113, 190, 334, 557, 717
PpsI	GAGTC	5 cut(s)	113, 190, 334, 557, 717
PpuMI	RGGWCCY	2 cut(s)	788, 2606
PscI	ACATGT	1 cut(s)	3063
PshAI	GACNNNNGTC	1 cut(s)	276
PsiI	TTATAA	1 cut(s)	1946
Psp5II	RGGWCCY	2 cut(s)	788, 2606
Psp6I	CCWGG	3 cut(s)	66, 535, 1425
PspEI	GGTNACC	1 cut(s)	1184
PspFI	CCCAGC	1 cut(s)	698
PspGI	CCWGG	3 cut(s)	66, 535, 1425
PspLI	CGTACG	1 cut(s)	900
PspPI	GGNCC	3 cut(s)	583, 788, 2606
PspPPI	RGGWCCY	2 cut(s)	788, 2606
PstNI	CAGNNNCTG	1 cut(s)	2063
PsuI	RGATCY	5 cut(s)	372, 415, 1132, 1809, 2953
PsyI	GACNNNGTC	1 cut(s)	427
PvuII	CAGCTG	2 cut(s)	2060, 2735
RruI	TCGCGA	1 cut(s)	103
RsaI	GTAC	7 cut(s)	242, 511, 902, 1467, 1821, 2687, 2939
RsaNI	GTAC	7 cut(s)	241, 510, 901, 1466, 1820, 2686, 2938
RseI	CAYNNNNRTG	4 cut(s)	293, 804, 2241, 2945
SalI	GTCGAC	1 cut(s)	4
SapI	GCTCTTC	1 cut(s)	27
Sau96I	GGNCC	3 cut(s)	583, 788, 2606
ScaI	AGTACT	1 cut(s)	511
SchI	GAGTC	5 cut(s)	114, 191, 335, 557, 718
ScrFI	CCNGG	7 cut(s)	68, 389, 402, 537, 1427, 1786, 2639
SduI	GDGCHC	9 cut(s)	96, 533, 630, 673, 811, 1042, 1422, 1639, 2088
SfaNI	GCATC	7 cut(s)	1232, 1468, 1739, 1747, 1779, 2013, 3019
SfcI	CTRYAG	1 cut(s)	2055
Sfr274I	CTCGAG	1 cut(s)	2099
SfuI	TTCGAA	1 cut(s)	2079
SinI	GGWCC	3 cut(s)	583, 788, 2606
SlaI	CTCGAG	1 cut(s)	2099
SmiMI	CAYNNNNRTG	4 cut(s)	293, 804, 2241, 2945
SmlI	CTYRAG	8 cut(s)	310, 629, 998, 1553, 2099, 2415, 2451, 2672
SmoI	CTYRAG	8 cut(s)	310, 629, 998, 1553, 2099, 2415, 2451, 2672
SpeI	ACTAGT	2 cut(s)	512, 776
SphI	GCATGC	1 cut(s)	3151
SspI	AATATT	3 cut(s)	1537, 2197, 2461
SspMI	CTAG	9 cut(s)	507, 513, 777, 1463, 1670, 1868, 2070, 2262, 3183
StyD4I	CCNGG	7 cut(s)	66, 387, 400, 535, 1425, 1784, 2637
StyI	CCWWGG	3 cut(s)	1761, 2859, 2880
TaaI	ACNGT	8 cut(s)	200, 292, 1190, 2094, 2166, 2986, 3044, 3123
TaiI	ACGT	2 cut(s)	1825, 1902
TatI	WGTACW	4 cut(s)	509, 1465, 2685, 2937
TauI	GCSGC	5 cut(s)	131, 221, 232, 480, 1368
TfiI	GAWTC	7 cut(s)	60, 136, 718, 733, 2116, 2350, 2979
TscAI	CASTG	6 cut(s)	533, 811, 863, 1102, 2155, 2785
TseFI	GTSAC	2 cut(s)	1184, 2189
Tsp45I	GTSAC	2 cut(s)	1184, 2189
TspGWI	ACGGA	5 cut(s)	95, 1628, 2177, 2534, 2732
TspRI	CASTG	6 cut(s)	533, 811, 863, 1102, 2155, 2785
Tth111I	GACNNNGTC	1 cut(s)	427
Vha464I	CTTAAG	1 cut(s)	2672
VpaK11BI	GGWCC	3 cut(s)	583, 788, 2606
XapI	RAATTY	8 cut(s)	649, 871, 1138, 1724, 1848, 1928, 2290, 2918
XceI	RCATGY	5 cut(s)	1084, 1223, 1477, 3067, 3151
XhoI	CTCGAG	1 cut(s)	2099
XmiI	GTMKAC	5 cut(s)	5, 111, 2136, 2407, 2988
XmnI	GAANNNNTTC	1 cut(s)	2895
XspI	CTAG	9 cut(s)	507, 513, 777, 1463, 1670, 1868, 2070, 2262, 3183
ZrmI	AGTACT	1 cut(s)	511
Zsp2I	ATGCAT	1 cut(s)	1225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.