Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0004171

Physical Location & Seq

Genomic Coordinates Forward (+)

49474709 .. 49477435

Gene Structure 2727 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00034160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGGTAATTTCGGCCCTGTCGAACCACCTTTTCAGTACCTAGTTTGCTGCTACAAACGTGCCCATGATGAGTGTAAGAAGATTACTTCTGTGAAACATAAGGATTTGAGGTTTTATATGGAATCTATTATTAGGCAAGCCCAGAAGCTGTCGGTTTCGTATGGTAGGATTCACTTGGGGAACCCGGAAGCGTTTCCTAGTCCAGGTGAGAATTCAAATAACTCAATAAGTGCGTCCCCTTTGTTGCCGTTGATTTTCTCAGAGGTTGCAAATTCAGTGGATGGATTTGGAGGAAGCAGTAGTAGCCCACCTGGGTTTTTGGATGAGTTCTTTCGTGACTCGGATTTTGATATGTTGGACCCCATTTTGAAGGGTTTGTATGAAGAACTTACGGAGACTGTGCAAAATGTTTCGGCACTTGGAAACTTTCAGCAGCCATTGAGGGCTCTGTTGTTTTTGGTTAGTTTCCCAGCTGGTGCCAAGTCTCTTGTGAATCACCCAAGGTGGATTCCCAAAGGAGTCTTGAATGGCCGTGGTATTGAAAAGACAAGTATTTTAGGTCCTTTCTTTCATGTCAGTGCCCTGTCTGACCATCCTTTTTCTGTTAGTGATTTCTTTTCAGAAGGTTCTAACACAGCTGATAAATCTTCGTACGTGACGATTAAGAAAGTGATGAACAATTTATATGATGGCCTCGCACAAGTTCTCCTCACGCTACTGAAAAATCCAGACACCCGCGAGAAAGTGCTGGAGTATCTTGCTGTGGTTATCAACAAGAACTCATCAAGAGCTCATATGCAAGTGGATCCTCTTTCCTGCGCAAGTTCGGGGATGTTTGTCAATCTCAGTGCGGTCATGCTTCGGCTTTGCGGGCCGCTTTTAGATGCAAACTTAACCAAAAGAGACAAAATTTATCCGAAATATGTGTTCTCAGGTAACCGTCTGGAGTTGAGAGGTTTGACAGCTCTACATGCAACATCTGAGGAAGTTACTGAATGGATTAGTAAAGATAACATGAGTGCTCAATATTCTATTAATTGTGAGTGCTTCTTCATGACTGCGAGGGTGCTCAATCTGGGCTTATTGAAAGCATTTTCTGTCCATAAACATTTAGAACAGAACATCCGCAGGTCTAAAGAAGCACTTAATGCCATGAAAGACCAGGGTTCTCCACAGTTGAACACTAACATAGCTCAGCTCGAGAAAGAAATAAAGTTGTTAGAACAGGAAAGGCATTGCTACGAAGCTCAGATAGTAAGGGATGAACCACTTGTACAGCAGGCGATTTCTTTCTACCGGTTGATGGTGGTGTGGCTAGGCGGCTTGGTTGGTGGATTTAAAATGCCTCTGCCATCAACGTGCCCCGTAGAATTTGCTTCTATGCCAGAGCATTTTGTGGCAGATGCCATGGAGTTGCTTTTATTTGCTTCCCGGATTCCAAAAGCATTCAGTGGGATCTTGTTGGATGATTTTATGGACTTCATAATAATGTTCATGGCAAGTCCGGAATATATCAGGAACCCTTATCTAAGATCAAAGATGGTTGAAGTCCTCAACTGCTTGATGCCGTGTGGAAGTCAACCATCTGCTGCTCCTCCTACCCTGTTCAAAGGGCACAAACTGTCTCTTGAGTATCTTGTAAGGAATCTCTTGAAACTATATGTGGATGTTGAGTTGACTGGCACACAGTTCTATGACAAGTTTAACATCCGGCACAAAATTGCTATGCTTCTCGAGTATCTGTGGCAAGTACCTAGTCATCGAAATGCTTGGAAAAAGGTTGCTAAGGAAGAGGAGAAGGGCGTATATTTGAATTACTTAAACTTCTTGATCAATGACAGTATATATCTTCTGGATAACAGTTTTAAAACGATTCTTGAACACAAACAACTGGAAGCCGAGATGTCAAACAATGCAGAATGGAAGCGTCAACCTGCTCAAGAGAGGAAGGAGAAAGCTCGCCTCTTCCACTCTCAGGAAAAGATTATCCAAACTCATTTGACGTTGGCAAATGAAGATCTGAATACGCTGGCATTTACTTCAGAACAGATTACAGCTCCTTTCCTACTCCCAGAGATGGTTGAAAGGGTGACAAGCATGCTGAATTATTTTTTATTGCAATTGGTAGGTCCTAAAAGAAAATCTCTTAATTTGAAAGACCCCGAGAAATGTAAGTTTCACCCAGAGCAATTGATCAAGCAGATTGTCTACATATATGTTCATCTGGCAAGAGGCGATACAGACAATACTTTTGCAGCTGCCATCTCAAAAGATGGTAGGTCTTACAATGACCAATTGTTTAGTGCTGCCGCGGATGTCTTAAGGAAAATTGATGAAGATGGGAGGGTCATACAAGAGTTTGTTGAGCTTGGTTCCAAAGCTGAGGTTGCTGCTTCGGAGGCCTTGGACGATGAAGCTGCACTTGGAGATATACCAGATGAGTTCCTAGACCCAATTCAGTACACTTTAATGAAGGATCCAGTTATCCTACCTTCTTCAAGAATCACAATAGACCGGCCAGTCATTCAAAGGCATCTTCTTAGTGATAATACTGATCCATTCAATCGTTCCCATCTTACTGCGGACATGTTGATACCAGACAATGAATTGAAAGCAAAAATACAAGAGTTCATTAGGTCCCAAGAGTCGAACAGTGGGAAAAGCCTAAGCATGCAGAGCAGCAAGACTACATTAATTACCAATTCACCACAGCATATGGTATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0030433 GO:0032446 GO:0033554 GO:0034450 GO:0034976 GO:0036211 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04120 ko04141 map04120 map04141

Pfam Domains

Protein Families

U-box Ufd2P_core

Protein Analysis

908

Amino Acids

102.63

Weight (kDa)

6.19

Isoelectric Point (pI)

42.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	186 - 795	1.5e-181	Ubiquitin elongating factor core
U-box	PF04564	811 - 882	1.3e-30	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15400
fragaria_vesca	FvH4_3g25920 FvH4_3g25930 FvH4_6g47550 FvH4_6g47550 FvH4_6g47550
malus_domestica	MD09G1060800.v1.1 MD17G1056200.v1.1
prunus_persica	Prupe.3G259400_v2.0.a1
pyrus_communis	pycom111g05110 pycom17g05530
rosa_chinensis	RchiOBHm_Chr2g0166651 RchiOBHm_Chr5g0047221
rosa_laevigata	RLG00000021638 RLG00000034480
rosa_multiflora	Rmu_sc0002310.1_g000029 Rmu_sc0005232.1_g000002
rosa_roxburghii	Rroxscaffold_1G00034160 Rroxscaffold_2G00084330 Rroxscaffold_2G00084640
rosa_rugosa	Rorug02G0524700 Rorug05G0235100
rosa_samantha	Rh2AG590900 Rh2BG602800 Rh2CG573700 Rh2DG613600 Rh5AG314000 Rh5BG322500 Rh5CG349700 Rh5CG349800 Rh5DG333500
rosa_wichuraiana	Rw2G049220 Rw5G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2519
Acc16I	TGCGCA	1 cut(s)	820
Acc36I	ACCTGC	2 cut(s)	1119, 1942
AccB1I	GGYRCC	1 cut(s)	476
AccI	GTMKAC	1 cut(s)	2208
AccII	CGCG	2 cut(s)	738, 2312
AccIII	TCCGGA	1 cut(s)	1504
AciI	CCGC	9 cut(s)	736, 851, 870, 875, 1126, 1320, 2310, 2312, 2582
AclWI	GGATC	6 cut(s)	800, 813, 1463, 2471, 2484, 2549
AcoI	YGGCCR	2 cut(s)	529, 2516
AcsI	RAATTY	4 cut(s)	211, 271, 909, 1370
AcuI	CTGAAG	1 cut(s)	2025
AfaI	GTAC	5 cut(s)	38, 653, 1275, 1752, 2462
AfiI	CCNNNNNNNGG	4 cut(s)	203, 1303, 1975, 2077
AflII	CTTAAG	1 cut(s)	2320
AflIII	ACRYGT	1 cut(s)	2586
AgeI	ACCGGT	1 cut(s)	1296
AjnI	CCWGG	3 cut(s)	202, 310, 1161
AjuI	GAANNNNNNNTTGG	2 cut(s)	2406, 2438
AloI	GAACNNNNNNTCC	2 cut(s)	1107, 1139
Alw21I	GWGCWC	3 cut(s)	793, 1024, 1071
Alw26I	GTCTC	4 cut(s)	389, 489, 897, 1629
AlwI	GGATC	6 cut(s)	800, 813, 1463, 2471, 2484, 2549
AlwNI	CAGNNNCTG	1 cut(s)	148
Ama87I	CYCGRG	3 cut(s)	1199, 1733, 2162
Aor13HI	TCCGGA	1 cut(s)	1504
AoxI	GGCC	6 cut(s)	13, 529, 691, 872, 2400, 2516
ApeKI	GCWGC	9 cut(s)	48, 433, 1589, 2255, 2258, 2306, 2390, 2417, 2679
ApoI	RAATTY	4 cut(s)	211, 271, 909, 1370
ArsI	GACNNNNNNTTYG	4 cut(s)	329, 361, 2234, 2266
AseI	ATTAAT	2 cut(s)	1035, 2693
AsiGI	ACCGGT	1 cut(s)	1296
Asp700I	GAANNNNTTC	1 cut(s)	192
AspLEI	GCGC	1 cut(s)	821
AspS9I	GGNCC	6 cut(s)	14, 358, 560, 872, 2129, 2637
AsuC2I	CCSGG	2 cut(s)	185, 1432
AsuHPI	GGTGA	5 cut(s)	218, 488, 2101, 2171, 2697
AvaI	CYCGRG	3 cut(s)	1199, 1733, 2162
AvaII	GGWCC	4 cut(s)	358, 560, 2129, 2637
BaeGI	GKGCMC	4 cut(s)	64, 583, 1364, 1617
BamHI	GGATCC	2 cut(s)	805, 2476
BanI	GGYRCC	1 cut(s)	476
BanII	GRGCYC	2 cut(s)	448, 793
Bbv12I	GWGCWC	3 cut(s)	793, 1024, 1071
BbvCI	CCTCAGC	1 cut(s)	2382
BbvI	GCAGC	9 cut(s)	35, 445, 1576, 2245, 2267, 2293, 2377, 2404, 2691
BceAI	ACGGC	3 cut(s)	232, 516, 1552
BcgI	CGANNNNNNTGC	2 cut(s)	2399, 2433
BciT130I	CCWGG	3 cut(s)	204, 312, 1163
BclI	TGATCA	2 cut(s)	1830, 2193
BcnI	CCSGG	2 cut(s)	185, 1432
BcoDI	GTCTC	4 cut(s)	389, 489, 897, 1629
BfaI	CTAG	6 cut(s)	41, 198, 1316, 1755, 2447, 2725
BfrI	CTTAAG	1 cut(s)	2320
BfuAI	ACCTGC	2 cut(s)	1119, 1942
BglII	AGATCT	1 cut(s)	2017
BlpI	GCTNAGC	1 cut(s)	1194
Bme1390I	CCNGG	5 cut(s)	185, 204, 312, 1163, 1432
Bme18I	GGWCC	4 cut(s)	358, 560, 2129, 2637
BmeT110I	CYCGRG	3 cut(s)	1199, 1733, 2162
BmgT120I	GGNCC	6 cut(s)	14, 358, 560, 872, 2129, 2637
BmiI	GGNNCC	8 cut(s)	182, 360, 478, 807, 1520, 2374, 2478, 2639
BmrFI	CCNGG	5 cut(s)	185, 204, 312, 1163, 1432
BmsI	GCATC	4 cut(s)	874, 1393, 1554, 2542
BpmI	CTGGAG	2 cut(s)	770, 965
Bpu10I	CCTNAGC	3 cut(s)	1785, 2382, 2666
Bpu1102I	GCTNAGC	1 cut(s)	1194
BpuEI	CTTGAG	2 cut(s)	1649, 1923
BpuMI	CCSGG	2 cut(s)	185, 1432
BsaAI	YACGTR	1 cut(s)	655
BsaJI	CCNNGG	7 cut(s)	311, 500, 532, 1162, 1407, 2310, 2403
BsaWI	WCCGGW	2 cut(s)	1296, 1504
BsaXI	ACNNNNNCTCC	2 cut(s)	690, 720
Bsc4I	CCNNNNNNNGG	4 cut(s)	203, 1303, 1975, 2077
Bse118I	RCCGGY	2 cut(s)	1296, 2514
Bse1I	ACTGG	4 cut(s)	1684, 1896, 2480, 2519
Bse3DI	GCAATG	1 cut(s)	1234
BseAI	TCCGGA	1 cut(s)	1504
BseBI	CCWGG	3 cut(s)	204, 312, 1163
BseDI	CCNNGG	7 cut(s)	311, 500, 532, 1162, 1407, 2310, 2403
BseLI	CCNNNNNNNGG	4 cut(s)	203, 1303, 1975, 2077
BseMI	GCAATG	1 cut(s)	1234
BseMII	CTCAG	8 cut(s)	273, 859, 945, 972, 1208, 1262, 1988, 2373
BseNI	ACTGG	4 cut(s)	1684, 1896, 2480, 2519
BseRI	GAGGAG	3 cut(s)	698, 1584, 1808
BseSI	GKGCMC	4 cut(s)	64, 583, 1364, 1617
BseXI	GCAGC	9 cut(s)	35, 445, 1576, 2245, 2267, 2293, 2377, 2404, 2691
BseYI	CCCAGC	1 cut(s)	469
BsgI	GTGCAG	1 cut(s)	2403
Bsh1236I	CGCG	2 cut(s)	738, 2312
BshFI	GGCC	6 cut(s)	15, 531, 693, 874, 2402, 2518
BshNI	GGYRCC	1 cut(s)	476
BshTI	ACCGGT	1 cut(s)	1296
BsiHKAI	GWGCWC	3 cut(s)	793, 1024, 1071
BsiHKCI	CYCGRG	3 cut(s)	1199, 1733, 2162
BsiSI	CCGG	6 cut(s)	185, 1297, 1432, 1505, 1711, 2515
BsiWI	CGTACG	1 cut(s)	651
BslFI	GGGAC	2 cut(s)	220, 2623
BslI	CCNNNNNNNGG	4 cut(s)	203, 1303, 1975, 2077
BsmAI	GTCTC	4 cut(s)	389, 489, 897, 1629
BsmFI	GGGAC	2 cut(s)	220, 2623
BsmI	GAATGC	1 cut(s)	1445
BsnI	GGCC	6 cut(s)	15, 531, 693, 874, 2402, 2518
BsoBI	CYCGRG	3 cut(s)	1199, 1733, 2162
Bsp1286I	GDGCHC	8 cut(s)	64, 448, 583, 793, 1024, 1071, 1364, 1617
Bsp13I	TCCGGA	1 cut(s)	1504
Bsp1407I	TGTACA	1 cut(s)	1273
Bsp143I	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
Bsp1720I	GCTNAGC	1 cut(s)	1194
Bsp19I	CCATGG	1 cut(s)	1407
BspACI	CCGC	9 cut(s)	736, 851, 870, 875, 1126, 1320, 2310, 2312, 2582
BspANI	GGCC	6 cut(s)	15, 531, 693, 874, 2402, 2518
BspCNI	CTCAG	8 cut(s)	272, 858, 944, 973, 1207, 1261, 1987, 2374
BspEI	TCCGGA	1 cut(s)	1504
BspFNI	CGCG	2 cut(s)	738, 2312
BspHI	TCATGA	1 cut(s)	1053
BspLI	GGNNCC	8 cut(s)	182, 360, 478, 807, 1520, 2374, 2478, 2639
BspMI	ACCTGC	2 cut(s)	1119, 1942
BspPI	GGATC	6 cut(s)	800, 813, 1463, 2471, 2484, 2549
BspT107I	GGYRCC	1 cut(s)	476
BspTI	CTTAAG	1 cut(s)	2320
BsrDI	GCAATG	1 cut(s)	1234
BsrFI	RCCGGY	2 cut(s)	1296, 2514
BsrGI	TGTACA	1 cut(s)	1273
BsrI	ACTGG	4 cut(s)	1684, 1896, 2480, 2519
BssAI	RCCGGY	2 cut(s)	1296, 2514
BssECI	CCNNGG	7 cut(s)	311, 500, 532, 1162, 1407, 2310, 2403
BssMI	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
BssT1I	CCWWGG	3 cut(s)	500, 1407, 2403
Bst2UI	CCWGG	3 cut(s)	204, 312, 1163
Bst4CI	ACNGT	8 cut(s)	400, 941, 1176, 1623, 1689, 1841, 1862, 2654
Bst6I	CTCTTC	2 cut(s)	1785, 1970
BstAFI	CTTAAG	1 cut(s)	2320
BstAPI	GCANNNNNTGC	1 cut(s)	1148
BstAUI	TGTACA	1 cut(s)	1273
BstBAI	YACGTR	1 cut(s)	655
BstC8I	GCNNGC	7 cut(s)	138, 872, 1281, 1960, 2031, 2099, 2672
BstDSI	CCRYGG	3 cut(s)	532, 1407, 2310
BstEII	GGTNACC	1 cut(s)	935
BstENI	CCTNNNNNAGG	1 cut(s)	201
BstFNI	CGCG	2 cut(s)	738, 2312
BstHHI	GCGC	1 cut(s)	821
BstKTI	GATC	8 cut(s)	808, 1458, 1535, 1833, 2020, 2196, 2479, 2557
BstMAI	GTCTC	4 cut(s)	389, 489, 897, 1629
BstMBI	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
BstMWI	GCNNNNNNNGC	7 cut(s)	303, 871, 971, 1148, 2387, 2399, 2676
BstNI	CCWGG	3 cut(s)	204, 312, 1163
BstNSI	RCATGY	4 cut(s)	974, 2101, 2590, 2674
BstPI	GGTNACC	1 cut(s)	935
BstSCI	CCNGG	5 cut(s)	183, 202, 310, 1161, 1430
BstSLI	GKGCMC	4 cut(s)	64, 583, 1364, 1617
BstUI	CGCG	2 cut(s)	738, 2312
BstV1I	GCAGC	9 cut(s)	35, 445, 1576, 2245, 2267, 2293, 2377, 2404, 2691
BstX2I	RGATCY	4 cut(s)	805, 1455, 2017, 2476
BstYI	RGATCY	4 cut(s)	805, 1455, 2017, 2476
BsuRI	GGCC	6 cut(s)	15, 531, 693, 874, 2402, 2518
BtgI	CCRYGG	3 cut(s)	532, 1407, 2310
BtsIMutI	CAGTG	5 cut(s)	282, 583, 853, 1456, 2659
BveI	ACCTGC	2 cut(s)	1119, 1942
Cac8I	GCNNGC	7 cut(s)	138, 872, 1281, 1960, 2031, 2099, 2672
CaiI	CAGNNNCTG	1 cut(s)	148
CciI	TCATGA	1 cut(s)	1053
CfoI	GCGC	1 cut(s)	821
Cfr10I	RCCGGY	2 cut(s)	1296, 2514
Cfr13I	GGNCC	6 cut(s)	14, 358, 560, 872, 2129, 2637
Cfr42I	CCGCGG	1 cut(s)	2313
CseI	GACGC	2 cut(s)	222, 1916
Csp6I	GTAC	5 cut(s)	37, 652, 1274, 1751, 2461
CspAI	ACCGGT	1 cut(s)	1296
CspCI	CAANNNNNGTGG	2 cut(s)	258, 293
CviQI	GTAC	5 cut(s)	37, 652, 1274, 1751, 2461
DpnI	GATC	8 cut(s)	807, 1457, 1534, 1832, 2019, 2195, 2478, 2556
DpnII	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
DraI	TTTAAA	2 cut(s)	1339, 1867
DrdI	GACNNNNNNGTC	1 cut(s)	2519
DseDI	GACNNNNNNGTC	1 cut(s)	2519
EaeI	YGGCCR	2 cut(s)	529, 2516
Eam1104I	CTCTTC	2 cut(s)	1785, 1970
EarI	CTCTTC	2 cut(s)	1785, 1970
Ecl136II	GAGCTC	1 cut(s)	791
Eco130I	CCWWGG	3 cut(s)	500, 1407, 2403
Eco147I	AGGCCT	1 cut(s)	2402
Eco24I	GRGCYC	2 cut(s)	448, 793
Eco47I	GGWCC	4 cut(s)	358, 560, 2129, 2637
Eco53kI	GAGCTC	1 cut(s)	791
Eco57I	CTGAAG	1 cut(s)	2025
Eco88I	CYCGRG	3 cut(s)	1199, 1733, 2162
Eco91I	GGTNACC	1 cut(s)	935
EcoICRI	GAGCTC	1 cut(s)	791
EcoNI	CCTNNNNNAGG	1 cut(s)	201
EcoO109I	RGGNCCY	3 cut(s)	560, 2129, 2637
EcoO65I	GGTNACC	1 cut(s)	935
EcoRI	GAATTC	1 cut(s)	211
EcoRII	CCWGG	3 cut(s)	202, 310, 1161
EcoT14I	CCWWGG	3 cut(s)	500, 1407, 2403
EcoT38I	GRGCYC	2 cut(s)	448, 793
ErhI	CCWWGG	3 cut(s)	500, 1407, 2403
FalI	AAGNNNNNCTT	4 cut(s)	1128, 1160, 2406, 2438
FaqI	GGGAC	2 cut(s)	220, 2623
FauI	CCCGC	2 cut(s)	743, 863
FauNDI	CATATG	2 cut(s)	795, 2715
FbaI	TGATCA	2 cut(s)	1830, 2193
FblI	GTMKAC	1 cut(s)	2208
FriOI	GRGCYC	2 cut(s)	448, 793
FspBI	CTAG	6 cut(s)	41, 198, 1316, 1755, 2447, 2725
FspI	TGCGCA	1 cut(s)	820
GlaI	GCGC	1 cut(s)	820
GsaI	CCCAGC	1 cut(s)	473
GsuI	CTGGAG	2 cut(s)	770, 965
HaeIII	GGCC	6 cut(s)	15, 531, 693, 874, 2402, 2518
HapII	CCGG	6 cut(s)	185, 1297, 1432, 1505, 1711, 2515
HgaI	GACGC	2 cut(s)	222, 1916
HhaI	GCGC	1 cut(s)	821
Hin6I	GCGC	1 cut(s)	819
HinP1I	GCGC	1 cut(s)	819
HincII	GTYRAC	3 cut(s)	1580, 1677, 1931
HindII	GTYRAC	3 cut(s)	1580, 1677, 1931
HpaII	CCGG	6 cut(s)	185, 1297, 1432, 1505, 1711, 2515
HphI	GGTGA	5 cut(s)	218, 488, 2101, 2171, 2697
Hpy166II	GTNNAC	5 cut(s)	1580, 1677, 1931, 2209, 2463
Hpy8I	GTNNAC	5 cut(s)	1580, 1677, 1931, 2209, 2463
HpyAV	CCTTC	6 cut(s)	364, 617, 1792, 1942, 2467, 2502
HpyCH4III	ACNGT	8 cut(s)	400, 941, 1176, 1623, 1689, 1841, 1862, 2654
HpyCH4IV	ACGT	4 cut(s)	58, 654, 1358, 2003
HpyF10VI	GCNNNNNNNGC	7 cut(s)	303, 871, 971, 1148, 2387, 2399, 2676
HpySE526I	ACGT	4 cut(s)	58, 654, 1358, 2003
HspAI	GCGC	1 cut(s)	819
Kpn2I	TCCGGA	1 cut(s)	1504
Ksp22I	TGATCA	2 cut(s)	1830, 2193
KspI	CCGCGG	1 cut(s)	2313
Kzo9I	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
LmnI	GCTCC	2 cut(s)	1597, 2062
Lsp1109I	GCAGC	9 cut(s)	35, 445, 1576, 2245, 2267, 2293, 2377, 2404, 2691
LweI	GCATC	4 cut(s)	874, 1393, 1554, 2542
MaeI	CTAG	6 cut(s)	41, 198, 1316, 1755, 2447, 2725
MaeII	ACGT	4 cut(s)	58, 654, 1358, 2003
MaeIII	GTNAC	5 cut(s)	335, 655, 935, 988, 2089
MalI	GATC	8 cut(s)	807, 1457, 1534, 1832, 2019, 2195, 2478, 2556
MboI	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
MfeI	CAATTG	3 cut(s)	2120, 2189, 2294
MflI	RGATCY	4 cut(s)	805, 1455, 2017, 2476
MhlI	GDGCHC	8 cut(s)	64, 448, 583, 793, 1024, 1071, 1364, 1617
MlyI	GAGTC	3 cut(s)	332, 528, 2654
MmeI	TCCRAC	2 cut(s)	336, 1443
MroI	TCCGGA	1 cut(s)	1504
MroXI	GAANNNNTTC	1 cut(s)	192
MslI	CAYNNNNRTG	5 cut(s)	576, 1357, 1487, 1764, 2468
MspA1I	CMGCKG	4 cut(s)	473, 638, 2258, 2312
MspCI	CTTAAG	1 cut(s)	2320
MspI	CCGG	6 cut(s)	185, 1297, 1432, 1505, 1711, 2515
MspR9I	CCNGG	5 cut(s)	185, 204, 312, 1163, 1432
MunI	CAATTG	3 cut(s)	2120, 2189, 2294
Mva1269I	GAATGC	1 cut(s)	1445
MvaI	CCWGG	3 cut(s)	204, 312, 1163
MvnI	CGCG	2 cut(s)	738, 2312
MwoI	GCNNNNNNNGC	7 cut(s)	303, 871, 971, 1148, 2387, 2399, 2676
NciI	CCSGG	2 cut(s)	185, 1432
NcoI	CCATGG	1 cut(s)	1407
NdeI	CATATG	2 cut(s)	795, 2715
NdeII	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
NlaIV	GGNNCC	8 cut(s)	182, 360, 478, 807, 1520, 2374, 2478, 2639
NmeAIII	GCCGAG	1 cut(s)	1924
NmuCI	GTSAC	3 cut(s)	335, 655, 2089
NsbI	TGCGCA	1 cut(s)	820
NspI	RCATGY	4 cut(s)	974, 2101, 2590, 2674
PaeI	GCATGC	2 cut(s)	2101, 2674
PaeR7I	CTCGAG	2 cut(s)	1199, 1733
PagI	TCATGA	1 cut(s)	1053
PceI	AGGCCT	1 cut(s)	2402
PciI	ACATGT	1 cut(s)	2586
PcsI	WCGNNNNNNNCGW	2 cut(s)	18, 656
PctI	GAATGC	1 cut(s)	1445
PdmI	GAANNNNTTC	1 cut(s)	192
PfeI	GAWTC	8 cut(s)	122, 169, 493, 508, 1435, 1645, 1873, 2502
Pfl23II	CGTACG	1 cut(s)	651
PfoI	TCCNGGA	1 cut(s)	1430
PinAI	ACCGGT	1 cut(s)	1296
PleI	GAGTC	3 cut(s)	332, 527, 2653
PpsI	GAGTC	3 cut(s)	332, 527, 2653
Ppu21I	YACGTR	1 cut(s)	655
PpuMI	RGGWCCY	3 cut(s)	560, 2129, 2637
PscI	ACATGT	1 cut(s)	2586
PshBI	ATTAAT	2 cut(s)	1035, 2693
Psp124BI	GAGCTC	1 cut(s)	793
Psp5II	RGGWCCY	3 cut(s)	560, 2129, 2637
Psp6I	CCWGG	3 cut(s)	202, 310, 1161
PspEI	GGTNACC	1 cut(s)	935
PspFI	CCCAGC	1 cut(s)	469
PspGI	CCWGG	3 cut(s)	202, 310, 1161
PspLI	CGTACG	1 cut(s)	651
PspN4I	GGNNCC	8 cut(s)	182, 360, 478, 807, 1520, 2374, 2478, 2639
PspPI	GGNCC	6 cut(s)	14, 358, 560, 872, 2129, 2637
PspPPI	RGGWCCY	3 cut(s)	560, 2129, 2637
PsrI	GAACNNNNNNTAC	2 cut(s)	1257, 1289
PstNI	CAGNNNCTG	1 cut(s)	148
PsuI	RGATCY	4 cut(s)	805, 1455, 2017, 2476
PvuII	CAGCTG	3 cut(s)	473, 638, 2258
RsaI	GTAC	5 cut(s)	38, 653, 1275, 1752, 2462
RsaNI	GTAC	5 cut(s)	37, 652, 1274, 1751, 2461
RseI	CAYNNNNRTG	5 cut(s)	576, 1357, 1487, 1764, 2468
SacI	GAGCTC	1 cut(s)	793
SacII	CCGCGG	1 cut(s)	2313
Sau3AI	GATC	8 cut(s)	805, 1455, 1532, 1830, 2017, 2193, 2476, 2554
Sau96I	GGNCC	6 cut(s)	14, 358, 560, 872, 2129, 2637
SchI	GAGTC	3 cut(s)	332, 528, 2654
ScrFI	CCNGG	5 cut(s)	185, 204, 312, 1163, 1432
SduI	GDGCHC	8 cut(s)	64, 448, 583, 793, 1024, 1071, 1364, 1617
SfaNI	GCATC	4 cut(s)	874, 1393, 1554, 2542
Sfr274I	CTCGAG	2 cut(s)	1199, 1733
Sfr303I	CCGCGG	1 cut(s)	2313
SgrBI	CCGCGG	1 cut(s)	2313
SinI	GGWCC	4 cut(s)	358, 560, 2129, 2637
SlaI	CTCGAG	2 cut(s)	1199, 1733
SmiMI	CAYNNNNRTG	5 cut(s)	576, 1357, 1487, 1764, 2468
SmlI	CTYRAG	5 cut(s)	1199, 1628, 1733, 1938, 2320
SmoI	CTYRAG	5 cut(s)	1199, 1628, 1733, 1938, 2320
SphI	GCATGC	2 cut(s)	2101, 2674
SseBI	AGGCCT	1 cut(s)	2402
SsiI	CCGC	9 cut(s)	736, 851, 870, 875, 1126, 1320, 2310, 2312, 2582
SspI	AATATT	1 cut(s)	1028
SspMI	CTAG	6 cut(s)	41, 198, 1316, 1755, 2447, 2725
SstI	GAGCTC	1 cut(s)	793
StuI	AGGCCT	1 cut(s)	2402
StyD4I	CCNGG	5 cut(s)	183, 202, 310, 1161, 1430
StyI	CCWWGG	3 cut(s)	500, 1407, 2403
TaaI	ACNGT	8 cut(s)	400, 941, 1176, 1623, 1689, 1841, 1862, 2654
TaiI	ACGT	4 cut(s)	61, 657, 1361, 2006
TaqI	TCGA	5 cut(s)	21, 1200, 1734, 1762, 2648
TatI	WGTACW	2 cut(s)	1273, 2460
TauI	GCSGC	3 cut(s)	877, 1323, 2312
TfiI	GAWTC	8 cut(s)	122, 169, 493, 508, 1435, 1645, 1873, 2502
TscAI	CASTG	5 cut(s)	282, 583, 853, 1456, 2659
TseFI	GTSAC	3 cut(s)	335, 655, 2089
TseI	GCWGC	9 cut(s)	48, 433, 1589, 2255, 2258, 2306, 2390, 2417, 2679
Tsp45I	GTSAC	3 cut(s)	335, 655, 2089
TspGWI	ACGGA	1 cut(s)	407
TspRI	CASTG	5 cut(s)	282, 583, 853, 1456, 2659
Vha464I	CTTAAG	1 cut(s)	2320
VpaK11BI	GGWCC	4 cut(s)	358, 560, 2129, 2637
VspI	ATTAAT	2 cut(s)	1035, 2693
XagI	CCTNNNNNAGG	1 cut(s)	201
XapI	RAATTY	4 cut(s)	211, 271, 909, 1370
XceI	RCATGY	4 cut(s)	974, 2101, 2590, 2674
XhoI	CTCGAG	2 cut(s)	1199, 1733
XmiI	GTMKAC	1 cut(s)	2208
XmnI	GAANNNNTTC	1 cut(s)	192
XspI	CTAG	6 cut(s)	41, 198, 1316, 1755, 2447, 2725

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.