Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0004171

Physical Location & Seq

Genomic Coordinates Reverse (-)

40325937 .. 40327871

Gene Structure 1935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG349700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGCAAGTGGATCCTCTTTCCTGCGCAAGTTCTGGCATGTTTGTCAATCTCAGTGCGGTCATGCTTCGGCTTTGCGGGCCGCTTTTAGATGCAAACTTAACCAAAAGAGACAACATTTATCCGAAATATGTGTTCTCGGGTAACCGTCTGGAGTTGAGAGGTTTGACAGCTCTACATGCAACATCTGAGGAAGTTACTGAATGGATTAGTAAAGATAACATGAGCGCTCAATATTCTATTAATTGTGAGTGCTTCTTCATGACTGCGAGGGTGCTCAATCTGGGTTTATTGAAAGCATTTTCTGACCATAAACATTTAGAACAGAACATTCGCAGGTCTAAAGAAGCACTTAATGCCATGAAAGACCAGGGTTTTCCACAGTTGAACACTAACATAGCTCAGCTCGAGAAAGAAATAAAGTGGTTAGAACAGGAAAGGCATTGCTACGAAGCTCAGATAGTAAGGGATGAACCACTTGTACAGCAGGCGATTTCTTTCTACCGGTTGATGGTGGTGTGGCTAGGCGGCTTGGTTGGTGGATTTAAAATGCCTCTGCCATCAACGTGCCCCGTAGAATTTGCATCTATGCCAGAGCATTTTGTGGAAGATGCCATGGAGTTGCTTTTATTTGCTTCCCGGATTCCAAAAGCATTTAGTGGGATCTTGTTGGATGATTTTATGAACTTCATAATAATGTTCATGGCAAGTCCGGAATATATCAGGAACCCTTATCTAAGATCAAAGATGGTTGAAGTCCTCAACTGCTTGATGCCGCGTGGAAGTCGACCATCTGCTGCTCCTCCTACCCTGTTCAAAGGGCACAAACTGTCTCTTGAGTATCTTGTAAGGAATCTCTTGAAACTATATGTGGATGTTGAGTTGACTAGCTCTCACACACAGTTCTATGACAAGTTTAACATCCGGCACAAAATTGCTAAGCTTCTGGAGTATCTGTGGCAAGTACCTAGTCATCGAAATGCTTGGAAAAAGGTTGCTAAGGAAGAGGAGAAGGGCGTATATTTGAATTACTTAAACTTCTTGATCAATGACAGTATATATCTTCTGGATAACAGTTTTAAAACGATTCTTGAACACAAACAACTGGAAGCCGAGATGTCAAACAATGCAGAATGGAAGCGTCGACCTGCTCAAGAGAGGAAGGAGAAAGCTCGCCTCTTCCACTCTCAGGAAAAGATTATCCAAACTCATTTGACATTCGCAAATGAAGATCTGAATACGCTGGCATTTACTTCAGAACAGATTACAGCTCCTTTCCTACTCCCAGAGATGGTTGAAAGGGTGGCAAGCATGCTGAATTATTTTTTGTTGCAATTGGTAGGTCCTAAAAGAAAATCTCTTAATTTGAAAGACCCCGAGAAATGTAAGTTTCACCCAGAGCAATTGATCGAGCAGATTGTCTACATATATGTTCATCTGGCAAGAGGCGATACAGACAATACTTTTGCAGCTGCCATCTCAAAAGATGGTAGGTCTTACAATGACCAATTGTTTAGCGCTGCCGCGGATGTCTTAAGGAAAATTGATGAAGATGGGAGGGTCATACAAGAGTTTGTTGAGCTTGGTTCCAAAGCCGAGGTTGCTGCTTCGGAGGCCTTGGACGCTGAAGCTGCACTTGGAGATATACCAGATGAGTTCCTAGACCCAATTCAGTACACTTTAATGAAGGATCCAGTTATCCTACCTTCTTCAAGAATCACAATAGACCGGCCAGTCATTCAGAGGCATCTTCTTAGTGATAATACTGATCCATTCAATCGTTCCCATCTTACTGCGGACATGTTGATACCAGACAATGAATTGAAAGCAAAAATACAAGAGTTCATTAGGTCCCAAGAGTCGAAGAGTACGGAAAAGCCTAAGCATGCAGAGCAGCAAGACTACAATACCAATTCACCACAACATATGGTATGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0030433 GO:0032446 GO:0033554 GO:0034450 GO:0034976 GO:0036211 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04120 ko04141 map04120 map04141

Pfam Domains

Protein Families

U-box Ufd2P_core

Protein Analysis

644

Amino Acids

73.98

Weight (kDa)

6.03

Isoelectric Point (pI)

46.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	1 - 532	4.7e-162	Ubiquitin elongating factor core
U-box	PF04564	548 - 619	8.3e-31	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15400
fragaria_vesca	FvH4_3g25920 FvH4_3g25930 FvH4_6g47550 FvH4_6g47550 FvH4_6g47550
malus_domestica	MD09G1060800.v1.1 MD17G1056200.v1.1
prunus_persica	Prupe.3G259400_v2.0.a1
pyrus_communis	pycom111g05110 pycom17g05530
rosa_chinensis	RchiOBHm_Chr2g0166651 RchiOBHm_Chr5g0047221
rosa_laevigata	RLG00000021638 RLG00000034480
rosa_multiflora	Rmu_sc0002310.1_g000029 Rmu_sc0005232.1_g000002
rosa_roxburghii	Rroxscaffold_1G00034160 Rroxscaffold_2G00084330 Rroxscaffold_2G00084640
rosa_rugosa	Rorug02G0524700 Rorug05G0235100
rosa_samantha	Rh2AG590900 Rh2BG602800 Rh2CG573700 Rh2DG613600 Rh5AG314000 Rh5BG322500 Rh5CG349700 Rh5CG349800 Rh5DG333500
rosa_wichuraiana	Rw2G049220 Rw5G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1730
Acc16I	TGCGCA	1 cut(s)	25
Acc36I	ACCTGC	2 cut(s)	324, 1153
AccI	GTMKAC	3 cut(s)	784, 1141, 1419
AccII	CGCG	2 cut(s)	775, 1523
AccIII	TCCGGA	1 cut(s)	709
AciI	CCGC	8 cut(s)	56, 75, 80, 525, 773, 1521, 1523, 1793
AclWI	GGATC	6 cut(s)	5, 18, 668, 1682, 1695, 1760
AcoI	YGGCCR	1 cut(s)	1727
AcsI	RAATTY	1 cut(s)	575
AcuI	CTGAAG	2 cut(s)	1236, 1644
AfaI	GTAC	4 cut(s)	480, 963, 1673, 1867
AfeI	AGCGCT	2 cut(s)	226, 1516
AfiI	CCNNNNNNNGG	3 cut(s)	508, 1186, 1288
AflII	CTTAAG	1 cut(s)	1531
AflIII	ACRYGT	1 cut(s)	1797
AgeI	ACCGGT	1 cut(s)	501
AjnI	CCWGG	1 cut(s)	366
AjuI	GAANNNNNNNTTGG	2 cut(s)	1617, 1649
Alw21I	GWGCWC	1 cut(s)	276
Alw26I	GTCTC	2 cut(s)	102, 834
AlwI	GGATC	6 cut(s)	5, 18, 668, 1682, 1695, 1760
Ama87I	CYCGRG	3 cut(s)	136, 404, 1373
Aor13HI	TCCGGA	1 cut(s)	709
Aor51HI	AGCGCT	2 cut(s)	226, 1516
AoxI	GGCC	3 cut(s)	77, 1611, 1727
ApeKI	GCWGC	7 cut(s)	794, 1466, 1469, 1517, 1601, 1628, 1891
ApoI	RAATTY	1 cut(s)	575
ArsI	GACNNNNNNTTYG	2 cut(s)	1445, 1477
AseI	ATTAAT	1 cut(s)	240
AsiGI	ACCGGT	1 cut(s)	501
AspLEI	GCGC	3 cut(s)	26, 227, 1517
AspS9I	GGNCC	3 cut(s)	77, 1340, 1848
AsuC2I	CCSGG	1 cut(s)	637
AsuHPI	GGTGA	2 cut(s)	1382, 1905
AvaI	CYCGRG	3 cut(s)	136, 404, 1373
AvaII	GGWCC	2 cut(s)	1340, 1848
BaeGI	GKGCMC	2 cut(s)	569, 822
BamHI	GGATCC	2 cut(s)	10, 1687
Bbv12I	GWGCWC	1 cut(s)	276
BbvI	GCAGC	7 cut(s)	781, 1456, 1478, 1504, 1588, 1615, 1903
BccI	CCATC	9 cut(s)	502, 565, 739, 796, 1282, 1478, 1481, 1544, 1791
BcgI	CGANNNNNNTGC	2 cut(s)	1583, 1617
BciT130I	CCWGG	1 cut(s)	368
BclI	TGATCA	1 cut(s)	1041
BcnI	CCSGG	1 cut(s)	637
BcoDI	GTCTC	2 cut(s)	102, 834
BfaI	CTAG	5 cut(s)	521, 885, 966, 1658, 1933
BfoI	RGCGCY	2 cut(s)	228, 1518
BfrI	CTTAAG	1 cut(s)	1531
BfuAI	ACCTGC	2 cut(s)	324, 1153
BglII	AGATCT	1 cut(s)	1228
BlpI	GCTNAGC	2 cut(s)	399, 936
Bme1390I	CCNGG	2 cut(s)	368, 637
Bme18I	GGWCC	2 cut(s)	1340, 1848
BmeT110I	CYCGRG	3 cut(s)	136, 404, 1373
BmgT120I	GGNCC	3 cut(s)	77, 1340, 1848
BmiI	GGNNCC	5 cut(s)	12, 725, 1585, 1689, 1850
BmrFI	CCNGG	2 cut(s)	368, 637
BmsI	GCATC	5 cut(s)	79, 590, 598, 759, 1753
BpmI	CTGGAG	2 cut(s)	170, 965
Bpu10I	CCTNAGC	2 cut(s)	996, 1878
Bpu1102I	GCTNAGC	2 cut(s)	399, 936
BpuEI	CTTGAG	2 cut(s)	854, 1134
BpuMI	CCSGG	1 cut(s)	637
BsaJI	CCNNGG	5 cut(s)	367, 612, 1521, 1593, 1614
BsaWI	WCCGGW	2 cut(s)	501, 709
BsaXI	ACNNNNNCTCC	2 cut(s)	938, 968
Bsc4I	CCNNNNNNNGG	3 cut(s)	508, 1186, 1288
Bse118I	RCCGGY	2 cut(s)	501, 1725
Bse1I	ACTGG	3 cut(s)	1107, 1691, 1730
Bse3DI	GCAATG	1 cut(s)	439
BseAI	TCCGGA	1 cut(s)	709
BseBI	CCWGG	1 cut(s)	368
BseDI	CCNNGG	5 cut(s)	367, 612, 1521, 1593, 1614
BseGI	GGATG	5 cut(s)	472, 676, 877, 918, 1531
BseLI	CCNNNNNNNGG	3 cut(s)	508, 1186, 1288
BseMI	GCAATG	1 cut(s)	439
BseMII	CTCAG	5 cut(s)	64, 177, 413, 467, 1199
BseNI	ACTGG	3 cut(s)	1107, 1691, 1730
BseRI	GAGGAG	2 cut(s)	789, 1019
BseSI	GKGCMC	2 cut(s)	569, 822
BseXI	GCAGC	7 cut(s)	781, 1456, 1478, 1504, 1588, 1615, 1903
BsgI	GTGCAG	1 cut(s)	1614
Bsh1236I	CGCG	2 cut(s)	775, 1523
BshFI	GGCC	3 cut(s)	79, 1613, 1729
BshTI	ACCGGT	1 cut(s)	501
BsiHKAI	GWGCWC	1 cut(s)	276
BsiHKCI	CYCGRG	3 cut(s)	136, 404, 1373
BsiSI	CCGG	5 cut(s)	502, 637, 710, 922, 1726
BslFI	GGGAC	1 cut(s)	1834
BslI	CCNNNNNNNGG	3 cut(s)	508, 1186, 1288
BsmAI	GTCTC	2 cut(s)	102, 834
BsmFI	GGGAC	1 cut(s)	1834
BsnI	GGCC	3 cut(s)	79, 1613, 1729
BsoBI	CYCGRG	3 cut(s)	136, 404, 1373
Bsp1286I	GDGCHC	3 cut(s)	276, 569, 822
Bsp13I	TCCGGA	1 cut(s)	709
Bsp1407I	TGTACA	1 cut(s)	478
Bsp143I	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
Bsp1720I	GCTNAGC	2 cut(s)	399, 936
Bsp19I	CCATGG	1 cut(s)	612
BspACI	CCGC	8 cut(s)	56, 75, 80, 525, 773, 1521, 1523, 1793
BspANI	GGCC	3 cut(s)	79, 1613, 1729
BspCNI	CTCAG	5 cut(s)	63, 178, 412, 466, 1198
BspEI	TCCGGA	1 cut(s)	709
BspFNI	CGCG	2 cut(s)	775, 1523
BspHI	TCATGA	1 cut(s)	258
BspLI	GGNNCC	5 cut(s)	12, 725, 1585, 1689, 1850
BspMI	ACCTGC	2 cut(s)	324, 1153
BspPI	GGATC	6 cut(s)	5, 18, 668, 1682, 1695, 1760
BspTI	CTTAAG	1 cut(s)	1531
BsrDI	GCAATG	1 cut(s)	439
BsrFI	RCCGGY	2 cut(s)	501, 1725
BsrGI	TGTACA	1 cut(s)	478
BsrI	ACTGG	3 cut(s)	1107, 1691, 1730
BssAI	RCCGGY	2 cut(s)	501, 1725
BssECI	CCNNGG	5 cut(s)	367, 612, 1521, 1593, 1614
BssMI	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
BssT1I	CCWWGG	2 cut(s)	612, 1614
Bst2UI	CCWGG	1 cut(s)	368
Bst4CI	ACNGT	6 cut(s)	146, 381, 828, 900, 1052, 1073
Bst6I	CTCTTC	3 cut(s)	996, 1181, 1856
BstAFI	CTTAAG	1 cut(s)	1531
BstAPI	GCANNNNNTGC	1 cut(s)	353
BstAUI	TGTACA	1 cut(s)	478
BstC8I	GCNNGC	7 cut(s)	77, 486, 1171, 1242, 1306, 1310, 1884
BstDSI	CCRYGG	2 cut(s)	612, 1521
BstEII	GGTNACC	1 cut(s)	140
BstF5I	GGATG	5 cut(s)	472, 676, 877, 918, 1531
BstFNI	CGCG	2 cut(s)	775, 1523
BstH2I	RGCGCY	2 cut(s)	228, 1518
BstHHI	GCGC	3 cut(s)	26, 227, 1517
BstKTI	GATC	8 cut(s)	13, 663, 740, 1044, 1231, 1407, 1690, 1768
BstMAI	GTCTC	2 cut(s)	102, 834
BstMBI	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
BstMWI	GCNNNNNNNGC	8 cut(s)	76, 176, 353, 1598, 1610, 1619, 1628, 1888
BstNI	CCWGG	1 cut(s)	368
BstNSI	RCATGY	5 cut(s)	40, 179, 1312, 1801, 1886
BstPI	GGTNACC	1 cut(s)	140
BstSCI	CCNGG	2 cut(s)	366, 635
BstSLI	GKGCMC	2 cut(s)	569, 822
BstUI	CGCG	2 cut(s)	775, 1523
BstV1I	GCAGC	7 cut(s)	781, 1456, 1478, 1504, 1588, 1615, 1903
BstX2I	RGATCY	4 cut(s)	10, 660, 1228, 1687
BstYI	RGATCY	4 cut(s)	10, 660, 1228, 1687
BsuRI	GGCC	3 cut(s)	79, 1613, 1729
BtgI	CCRYGG	2 cut(s)	612, 1521
BtsCI	GGATG	5 cut(s)	472, 676, 877, 918, 1531
BtsIMutI	CAGTG	1 cut(s)	58
BveI	ACCTGC	2 cut(s)	324, 1153
Cac8I	GCNNGC	7 cut(s)	77, 486, 1171, 1242, 1306, 1310, 1884
CciI	TCATGA	1 cut(s)	258
CfoI	GCGC	3 cut(s)	26, 227, 1517
Cfr10I	RCCGGY	2 cut(s)	501, 1725
Cfr13I	GGNCC	3 cut(s)	77, 1340, 1848
Cfr42I	CCGCGG	1 cut(s)	1524
CseI	GACGC	2 cut(s)	1127, 1628
Csp6I	GTAC	4 cut(s)	479, 962, 1672, 1866
CspAI	ACCGGT	1 cut(s)	501
CviQI	GTAC	4 cut(s)	479, 962, 1672, 1866
DpnI	GATC	8 cut(s)	12, 662, 739, 1043, 1230, 1406, 1689, 1767
DpnII	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
DraI	TTTAAA	2 cut(s)	544, 1078
DrdI	GACNNNNNNGTC	1 cut(s)	1730
DseDI	GACNNNNNNGTC	1 cut(s)	1730
EaeI	YGGCCR	1 cut(s)	1727
Eam1104I	CTCTTC	3 cut(s)	996, 1181, 1856
EarI	CTCTTC	3 cut(s)	996, 1181, 1856
Eco130I	CCWWGG	2 cut(s)	612, 1614
Eco147I	AGGCCT	1 cut(s)	1613
Eco47I	GGWCC	2 cut(s)	1340, 1848
Eco47III	AGCGCT	2 cut(s)	226, 1516
Eco57I	CTGAAG	2 cut(s)	1236, 1644
Eco88I	CYCGRG	3 cut(s)	136, 404, 1373
Eco91I	GGTNACC	1 cut(s)	140
EcoO109I	RGGNCCY	2 cut(s)	1340, 1848
EcoO65I	GGTNACC	1 cut(s)	140
EcoRII	CCWGG	1 cut(s)	366
EcoT14I	CCWWGG	2 cut(s)	612, 1614
ErhI	CCWWGG	2 cut(s)	612, 1614
FalI	AAGNNNNNCTT	4 cut(s)	333, 365, 1617, 1649
FaqI	GGGAC	1 cut(s)	1834
FauI	CCCGC	1 cut(s)	68
FauNDI	CATATG	1 cut(s)	1923
FbaI	TGATCA	1 cut(s)	1041
FblI	GTMKAC	3 cut(s)	784, 1141, 1419
FokI	GGATG	5 cut(s)	479, 683, 884, 905, 1538
FspBI	CTAG	5 cut(s)	521, 885, 966, 1658, 1933
FspI	TGCGCA	1 cut(s)	25
GlaI	GCGC	3 cut(s)	25, 226, 1516
GsuI	CTGGAG	2 cut(s)	170, 965
HaeII	RGCGCY	2 cut(s)	228, 1518
HaeIII	GGCC	3 cut(s)	79, 1613, 1729
HapII	CCGG	5 cut(s)	502, 637, 710, 922, 1726
HgaI	GACGC	2 cut(s)	1127, 1628
HhaI	GCGC	3 cut(s)	26, 227, 1517
Hin6I	GCGC	3 cut(s)	24, 225, 1515
HinP1I	GCGC	3 cut(s)	24, 225, 1515
HincII	GTYRAC	3 cut(s)	785, 882, 1142
HindII	GTYRAC	3 cut(s)	785, 882, 1142
HindIII	AAGCTT	1 cut(s)	938
HinfI	GANTC	5 cut(s)	640, 850, 1084, 1713, 1856
HpaII	CCGG	5 cut(s)	502, 637, 710, 922, 1726
HphI	GGTGA	2 cut(s)	1382, 1905
Hpy166II	GTNNAC	5 cut(s)	785, 882, 1142, 1420, 1674
Hpy188I	TCNGA	8 cut(s)	123, 187, 304, 456, 1233, 1255, 1609, 1740
Hpy8I	GTNNAC	5 cut(s)	785, 882, 1142, 1420, 1674
Hpy99I	CGWCG	1 cut(s)	1143
HpyAV	CCTTC	4 cut(s)	1003, 1153, 1678, 1713
HpyCH4III	ACNGT	6 cut(s)	146, 381, 828, 900, 1052, 1073
HpyCH4IV	ACGT	1 cut(s)	563
HpyCH4V	TGCA	9 cut(s)	4, 92, 179, 581, 1127, 1330, 1466, 1631, 1886
HpyF10VI	GCNNNNNNNGC	8 cut(s)	76, 176, 353, 1598, 1610, 1619, 1628, 1888
HpySE526I	ACGT	1 cut(s)	563
HspAI	GCGC	3 cut(s)	24, 225, 1515
Kpn2I	TCCGGA	1 cut(s)	709
Ksp22I	TGATCA	1 cut(s)	1041
KspI	CCGCGG	1 cut(s)	1524
Kzo9I	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
LmnI	GCTCC	2 cut(s)	802, 1273
Lsp1109I	GCAGC	7 cut(s)	781, 1456, 1478, 1504, 1588, 1615, 1903
LweI	GCATC	5 cut(s)	79, 590, 598, 759, 1753
MaeI	CTAG	5 cut(s)	521, 885, 966, 1658, 1933
MaeII	ACGT	1 cut(s)	563
MaeIII	GTNAC	2 cut(s)	140, 193
MalI	GATC	8 cut(s)	12, 662, 739, 1043, 1230, 1406, 1689, 1767
MboI	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
MfeI	CAATTG	3 cut(s)	1331, 1400, 1505
MflI	RGATCY	4 cut(s)	10, 660, 1228, 1687
MhlI	GDGCHC	3 cut(s)	276, 569, 822
MlyI	GAGTC	1 cut(s)	1865
MmeI	TCCRAC	1 cut(s)	648
MroI	TCCGGA	1 cut(s)	709
MslI	CAYNNNNRTG	4 cut(s)	562, 692, 975, 1679
MspA1I	CMGCKG	2 cut(s)	1469, 1523
MspCI	CTTAAG	1 cut(s)	1531
MspI	CCGG	5 cut(s)	502, 637, 710, 922, 1726
MspR9I	CCNGG	2 cut(s)	368, 637
MunI	CAATTG	3 cut(s)	1331, 1400, 1505
MvaI	CCWGG	1 cut(s)	368
MvnI	CGCG	2 cut(s)	775, 1523
MwoI	GCNNNNNNNGC	8 cut(s)	76, 176, 353, 1598, 1610, 1619, 1628, 1888
NciI	CCSGG	1 cut(s)	637
NcoI	CCATGG	1 cut(s)	612
NdeI	CATATG	1 cut(s)	1923
NdeII	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
NlaIV	GGNNCC	5 cut(s)	12, 725, 1585, 1689, 1850
NmeAIII	GCCGAG	2 cut(s)	1135, 1618
NsbI	TGCGCA	1 cut(s)	25
NspI	RCATGY	5 cut(s)	40, 179, 1312, 1801, 1886
PaeI	GCATGC	2 cut(s)	1312, 1886
PaeR7I	CTCGAG	1 cut(s)	404
PagI	TCATGA	1 cut(s)	258
PceI	AGGCCT	1 cut(s)	1613
PciI	ACATGT	1 cut(s)	1797
PfeI	GAWTC	4 cut(s)	640, 850, 1084, 1713
PfoI	TCCNGGA	1 cut(s)	635
PinAI	ACCGGT	1 cut(s)	501
PleI	GAGTC	1 cut(s)	1864
PpsI	GAGTC	1 cut(s)	1864
PpuMI	RGGWCCY	2 cut(s)	1340, 1848
PscI	ACATGT	1 cut(s)	1797
PshBI	ATTAAT	1 cut(s)	240
Psp5II	RGGWCCY	2 cut(s)	1340, 1848
Psp6I	CCWGG	1 cut(s)	366
PspEI	GGTNACC	1 cut(s)	140
PspGI	CCWGG	1 cut(s)	366
PspN4I	GGNNCC	5 cut(s)	12, 725, 1585, 1689, 1850
PspPI	GGNCC	3 cut(s)	77, 1340, 1848
PspPPI	RGGWCCY	2 cut(s)	1340, 1848
PsrI	GAACNNNNNNTAC	2 cut(s)	462, 494
PsuI	RGATCY	4 cut(s)	10, 660, 1228, 1687
PvuII	CAGCTG	1 cut(s)	1469
RsaI	GTAC	4 cut(s)	480, 963, 1673, 1867
RsaNI	GTAC	4 cut(s)	479, 962, 1672, 1866
RseI	CAYNNNNRTG	4 cut(s)	562, 692, 975, 1679
SacII	CCGCGG	1 cut(s)	1524
SalI	GTCGAC	2 cut(s)	783, 1140
Sau3AI	GATC	8 cut(s)	10, 660, 737, 1041, 1228, 1404, 1687, 1765
Sau96I	GGNCC	3 cut(s)	77, 1340, 1848
SchI	GAGTC	1 cut(s)	1865
ScrFI	CCNGG	2 cut(s)	368, 637
SduI	GDGCHC	3 cut(s)	276, 569, 822
SfaNI	GCATC	5 cut(s)	79, 590, 598, 759, 1753
Sfr274I	CTCGAG	1 cut(s)	404
Sfr303I	CCGCGG	1 cut(s)	1524
SgrBI	CCGCGG	1 cut(s)	1524
SinI	GGWCC	2 cut(s)	1340, 1848
SlaI	CTCGAG	1 cut(s)	404
SmiMI	CAYNNNNRTG	4 cut(s)	562, 692, 975, 1679
SmlI	CTYRAG	4 cut(s)	404, 833, 1149, 1531
SmoI	CTYRAG	4 cut(s)	404, 833, 1149, 1531
SphI	GCATGC	2 cut(s)	1312, 1886
SseBI	AGGCCT	1 cut(s)	1613
SsiI	CCGC	8 cut(s)	56, 75, 80, 525, 773, 1521, 1523, 1793
SspI	AATATT	1 cut(s)	233
SspMI	CTAG	5 cut(s)	521, 885, 966, 1658, 1933
StuI	AGGCCT	1 cut(s)	1613
StyD4I	CCNGG	2 cut(s)	366, 635
StyI	CCWWGG	2 cut(s)	612, 1614
TaaI	ACNGT	6 cut(s)	146, 381, 828, 900, 1052, 1073
TaiI	ACGT	1 cut(s)	566
TaqI	TCGA	6 cut(s)	405, 784, 973, 1141, 1407, 1859
TatI	WGTACW	2 cut(s)	478, 1671
TauI	GCSGC	4 cut(s)	82, 528, 775, 1523
TfiI	GAWTC	4 cut(s)	640, 850, 1084, 1713
TscAI	CASTG	1 cut(s)	58
TseI	GCWGC	7 cut(s)	794, 1466, 1469, 1517, 1601, 1628, 1891
TspGWI	ACGGA	1 cut(s)	1883
TspRI	CASTG	1 cut(s)	58
Vha464I	CTTAAG	1 cut(s)	1531
VpaK11BI	GGWCC	2 cut(s)	1340, 1848
VspI	ATTAAT	1 cut(s)	240
XapI	RAATTY	1 cut(s)	575
XceI	RCATGY	5 cut(s)	40, 179, 1312, 1801, 1886
XhoI	CTCGAG	1 cut(s)	404
XmiI	GTMKAC	3 cut(s)	784, 1141, 1419
XspI	CTAG	5 cut(s)	521, 885, 966, 1658, 1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.