Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004171

Physical Location & Seq

Genomic Coordinates Forward (+)

76923343 .. 76928973

Gene Structure 5631 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021638

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGTCGACCCCGAAACCGCAGAGGTCTAAAGAAGAGCTGGAGGACATAATCCTCCGGAAAATCTTCCAGGTCTCGCTGACGGAGCCGGCCGAGCCCAATTCCCGACTCGTCTACTTGGAGATGACGGCGGCGGAGATTCTCAGCGAGGGCAAGCCGATGAGGCTCACGCGCGACCTCATGGAGTCGATCCTGATCGACCGTTTAATGGGTAGCTTCGCCGCCGCCGCCGTGGAGCCGCCGTTTCAGTACCTAGTCGGGTGTTACCGACGCGCCTACGACGAGGGTCGGAAAATCACCGTCATGAAAGATAAGAACTTGAGGGCGGATATGGAGTCCGTGATTAAACAGGCGAAGAAGCTTTCGGTTTCGTATTGTAGGATCCATTTGGGGAACCCGGAGGCGTTTCCGGGTTCGGGTTCGGATCCGGCGACTGCGTCAAATGCGTCGCCGTTGCTGCCGTTGATTTTTTCCGAGGTGGGGAGCGGCTCGGTGGATGAGTTTGGAGGGAGTTCTAGTAGTAGTGGAGTTCAGTGCCCACCTGGGTTTTTGGATGAGTTCTTTAGGGACTCGGATTTCGATAGCTTGGACCCAATTTTGAAGGGATTGTATGAGGAGCTTAGGGAGATTGTGCTTAAGGTTTCGGCATTGGGGAATTTCCAGCAGCCATTGAGGGCTCTTTTGTATTTGGTTAGCTTTCCTGCTGGGGCTAAGAGTCTGGTGAATCACCCGTGGTGGATTCCGAAAGGTGTTTACTTGAATGGGAGGGTTATCGAAAGGACTAGTATTTTGGGTCCTTTTTTTCATGTCAGTGCTTTGCCGGATCATCCTATTTTCAAGAGCCAGCCGGATGTGGGGCAGCAGTGCTTTTCAGAAAATTCTAAGGATAGTGATCTGCGATCTTCGTACGTCACAATAAAGACACTTATGAACAGTTTATATGACGGCCTCGCAGAAGTTCTCCTCAAACTATTAAAAAATGCAGAGACCCGTGAGAGTGTTCTTGAGTATCTTGCTGAGGTTATCAACAAAAACTCATCAAGGGCTCATATTCAGGTTGATCCTCTTTCTTGTGCAAGTTCTGGCATGTTTGTCAATCTCAGTGCTGTCATGCTTCGACTTTGCGAGCCATTTCTGGATACAAATTTAACAAAAAGGGACAAAATTGATCCAAAATATGTATTCTATGGTGACCGTCTGGAGTTAAGAGGTTTGACTGCTCTACATGCATCATCTGAAGAAGTTACTGAATGGATTAACAAAGATAGTCTGGGGAATGCTGGTGGTGATGGTCAAAATCGCCTTTCACAATCCCAAGAAGCCACCAGTTCTGGCAGTAATATATCTCAACCTTCTAACGTGAAGCCAAGCGGCGAAAAATCTAAATCATTTATTTGTGAATGCTTCTTCATGACTGCGAGGGTGCTCAATCTGGGTTTATTGAAAGCATTATCTGACTATAAGCATCTAGTACAGGACATGCAGAGGTCTGAAGATACACTTAAAGCCTTGAAAAAACAGGCACCGTCTCCTCAATTGAAGATTGATATAGCTCGCCTTGAGAAAGAGTTAGAGTTCTTTAAGCAGGAGAAGCGTTGTCATGAAGCTCAGATACTCACGGATGAACCACTTTTAGAGCGTGCTCTTTCTTTCTACCGGTTGATGGTGGTGTGGCTAGTTCGCTTGGTTGGTGGATTTAAAATGCCTCTGCCGTCAACTTGCCCTGTGGAATTTGCATCTATGCCAGAACATTTTGTGGAAGATGCCATGGAGTTGCTTATATTCGCTTCCCGGATGCCAAAAGCATTTCATGGAATCTTGTTGGATGAATTTATGAACTTTATTATAATGTTCATGGCAAGTCCAGAATATATCAGGAACCCTTATCTAAGAGCAAAGATGGTTGAAGTCCTAAACTGCTTGATGCCCCGTCCAAGTCATCAATCACCTGCTGGTACAGCTGCTGCTGCCAGCCTCTTCGAAGGGCACCAACTGTCTCTCGAGTATCTTGTGAGGAATCTCTTGAAACTATATGTAGACATTGAGTTCACTGGTTCTCATAATCAGTTCTTTGACAAGTTTAACATCCGCCACAATATTGCTGAACTTCTCGAATATCTTTGGCAAGTCCCCAGTCATCGAAATGCTTGGAAACAGATTGCTAGGGAAGAAGAGAAAGGCGTATATTTGAATTTCTTAAATTTCTTGATCAATGATAGTATATATCTTCTGGATGAAAGTCTTAACAAGATTCTTGAACACAAAGAACTGGAGGCTGAGATGTCAAACACCGCAGAATGGGAGAGTAGACCTCCTCAAGAGAGGCAGGAGAGAACCCGCCTGTTCCACTCTCAAGAAAATATTATCAGAATTGATATGAAGTTGGCAAATGAAGATGTGAGTATGTTGGCATTTACTACAGAACAAATTACAGCTCCTTTCCTACTTCCTGAGATGGTTGAAAGGGTGGCAAGTATGCTCAATTATTTTTTGTTGCAATTAGTAGGTCCTCAAAGAAAATCTCTTAGTCTGAAAGACCCAGAGAAGTATGAGTTCCGCCCAAAGCAATTGCTTAAGCAGATTGTGTACATTTATGTTCATCTGGCAAGAGGTGATATGGAAAATATCTTTCCAGCTGCCATCTCAAAGGATGGTCGATCTTACAATGAGCAGTTGTTTAGTGCTGCTGCAGATGTCCTAAGGAGAATTGGTGAAGACGGGAGGGTCATACAAGAGTTTGTTGAGCTTGGTTCCAAAGCCAAGGTTGCTGCTTCTGAGGCCATGGACGTTGAAGCTGTTCTTGGGGATATACCAGATGAATTCCTTGACCCAATTCAGTACACTTTAATGAAGGATCCAGTTATCTTACCTTCTTCAAGAATCACGGTAGACCGGCCTGTCATTCAAAGGCATCTTCTTAGTGATAATAGTGATCCATTCAACCGTTCCCATCTTACTGCGGACATGTTGATACCGGATAATGATTTGAAGGCAAAAATACAAGAGTTTATTAGGTCGCAACAGTCGAAGCACGGGGAAAGCCTTAGCATGCAGAGTGATAAGGCAACAATACAAACCACATCTAGTGAAATGCTAATTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0030433 GO:0032446 GO:0033554 GO:0034450 GO:0034976 GO:0036211 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04120 ko04141 map04120 map04141

Pfam Domains

Protein Families

U-box Ufd2P_core

Protein Analysis

1034

Amino Acids

116.88

Weight (kDa)

5.52

Isoelectric Point (pI)

48.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ufd2P_core	PF10408	263 - 919	5.8e-201	Ubiquitin elongating factor core
U-box	PF04564	936 - 1007	1.1e-29	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15400
fragaria_vesca	FvH4_3g25920 FvH4_3g25930 FvH4_6g47550 FvH4_6g47550 FvH4_6g47550
malus_domestica	MD09G1060800.v1.1 MD17G1056200.v1.1
prunus_persica	Prupe.3G259400_v2.0.a1
pyrus_communis	pycom111g05110 pycom17g05530
rosa_chinensis	RchiOBHm_Chr2g0166651 RchiOBHm_Chr5g0047221
rosa_laevigata	RLG00000021638 RLG00000034480
rosa_multiflora	Rmu_sc0002310.1_g000029 Rmu_sc0005232.1_g000002
rosa_roxburghii	Rroxscaffold_1G00034160 Rroxscaffold_2G00084330 Rroxscaffold_2G00084640
rosa_rugosa	Rorug02G0524700 Rorug05G0235100
rosa_samantha	Rh2AG590900 Rh2BG602800 Rh2CG573700 Rh2DG613600 Rh5AG314000 Rh5BG322500 Rh5CG349700 Rh5CG349800 Rh5DG333500
rosa_wichuraiana	Rw2G049220 Rw5G029330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1844
AarI	CACCTGC	1 cut(s)	1954
AasI	GACNNNNNNGTC	2 cut(s)	2720, 2894
Acc36I	ACCTGC	1 cut(s)	1954
AccB1I	GGYRCC	2 cut(s)	1519, 1983
AccBSI	CCGCTC	1 cut(s)	483
AccI	GTMKAC	5 cut(s)	5, 111, 2034, 2305, 2886
AccII	CGCG	3 cut(s)	169, 171, 270
AccIII	TCCGGA	1 cut(s)	54
AcoI	YGGCCR	1 cut(s)	87
AcsI	RAATTY	8 cut(s)	652, 874, 1141, 1727, 1826, 2188, 2197, 2816
AcuI	CTGAAG	2 cut(s)	1254, 1509
AfaI	GTAC	6 cut(s)	248, 905, 1470, 1954, 2585, 2837
AfiI	CCNNNNNNNGG	6 cut(s)	205, 413, 477, 851, 1660, 2295
AflII	CTTAAG	2 cut(s)	632, 2570
AflIII	ACRYGT	1 cut(s)	2961
AgeI	ACCGGT	1 cut(s)	1653
AhlI	ACTAGT	1 cut(s)	779
AjnI	CCWGG	2 cut(s)	66, 538
AjuI	GAANNNNNNNTTGG	2 cut(s)	2781, 2813
Alw21I	GWGCWC	2 cut(s)	1425, 1642
Alw26I	GTCTC	4 cut(s)	76, 977, 1530, 1998
AlwNI	CAGNNNCTG	1 cut(s)	1961
Ama87I	CYCGRG	1 cut(s)	1997
Aor13HI	TCCGGA	1 cut(s)	54
AoxI	GGCC	4 cut(s)	87, 943, 2775, 2891
ApoI	RAATTY	8 cut(s)	652, 874, 1141, 1727, 1826, 2188, 2197, 2816
ArsI	GACNNNNNNTTYG	4 cut(s)	557, 589, 769, 801
AsiGI	ACCGGT	1 cut(s)	1653
Asp700I	GAANNNNTTC	2 cut(s)	62, 2793
AspLEI	GCGC	2 cut(s)	171, 272
AspS9I	GGNCC	3 cut(s)	586, 791, 2504
AsuC2I	CCSGG	3 cut(s)	395, 408, 1789
AsuHPI	GGTGA	8 cut(s)	286, 716, 730, 1199, 1295, 1935, 2621, 2720
AsuII	TTCGAA	1 cut(s)	1977
AvaI	CYCGRG	1 cut(s)	1997
AvaII	GGWCC	3 cut(s)	586, 791, 2504
AxyI	CCTNAGG	1 cut(s)	2696
BaeGI	GKGCMC	2 cut(s)	536, 1986
BamHI	GGATCC	3 cut(s)	378, 421, 2851
BanI	GGYRCC	2 cut(s)	1519, 1983
BanII	GRGCYC	3 cut(s)	96, 676, 1045
BarI	GAAGNNNNNNTAC	2 cut(s)	1593, 1625
BbsI	GAAGAC	1 cut(s)	2718
Bbv12I	GWGCWC	2 cut(s)	1425, 1642
BbvCI	CCTCAGC	1 cut(s)	1014
BccI	CCATC	7 cut(s)	1280, 1654, 1891, 2446, 2642, 2645, 2955
BceAI	ACGGC	7 cut(s)	141, 212, 223, 433, 442, 958, 1693
BciT130I	CCWGG	2 cut(s)	68, 540
BciVI	GTATCC	1 cut(s)	1129
BclI	TGATCA	1 cut(s)	2205
BcnI	CCSGG	3 cut(s)	395, 408, 1789
BcoDI	GTCTC	4 cut(s)	76, 977, 1530, 1998
BcuI	ACTAGT	1 cut(s)	779
BfaI	CTAG	7 cut(s)	251, 513, 780, 1466, 1673, 2160, 3081
BfmI	CTRYAG	2 cut(s)	2415, 2685
BfrI	CTTAAG	2 cut(s)	632, 2570
BfuAI	ACCTGC	1 cut(s)	1954
BfuI	GTATCC	1 cut(s)	1129
BglI	GCCNNNNNGGC	1 cut(s)	160
Bme1390I	CCNGG	5 cut(s)	68, 395, 408, 540, 1789
Bme18I	GGWCC	3 cut(s)	586, 791, 2504
BmeT110I	CYCGRG	1 cut(s)	1997
BmgT120I	GGNCC	3 cut(s)	586, 791, 2504
BmrFI	CCNGG	5 cut(s)	68, 395, 408, 540, 1789
BmrI	ACTGGG	1 cut(s)	2124
BmsI	GCATC	7 cut(s)	1235, 1471, 1742, 1750, 1782, 1911, 2917
BmuI	ACTGGG	1 cut(s)	2124
BoxI	GACNNNNGTC	1 cut(s)	282
BpiI	GAAGAC	1 cut(s)	2718
BplI	GAGNNNNNCTC	2 cut(s)	2293, 2325
BpmI	CTGGAG	3 cut(s)	59, 1217, 2288
Bpu10I	CCTNAGC	3 cut(s)	617, 1014, 3041
Bpu14I	TTCGAA	1 cut(s)	1977
BpuEI	CTTGAG	5 cut(s)	337, 1022, 1577, 2298, 2334
BpuMI	CCSGG	3 cut(s)	395, 408, 1789
BsaBI	GATNNNNATC	2 cut(s)	191, 888
BsaI	GGTCTC	2 cut(s)	76, 977
BsaJI	CCNNGG	7 cut(s)	228, 471, 539, 728, 1764, 2757, 2778
BsaWI	WCCGGW	3 cut(s)	54, 1653, 2971
BsaXI	ACNNNNNCTCC	6 cut(s)	224, 254, 499, 529, 2692, 2722
Bsc4I	CCNNNNNNNGG	6 cut(s)	205, 413, 477, 851, 1660, 2295
Bse118I	RCCGGY	3 cut(s)	85, 1653, 2889
Bse1I	ACTGG	5 cut(s)	1323, 2053, 2130, 2271, 2855
Bse21I	CCTNAGG	1 cut(s)	2696
Bse8I	GATNNNNATC	2 cut(s)	191, 888
BseAI	TCCGGA	1 cut(s)	54
BseBI	CCWGG	2 cut(s)	68, 540
BseDI	CCNNGG	7 cut(s)	228, 471, 539, 728, 1764, 2757, 2778
BseJI	GATNNNNATC	2 cut(s)	191, 888
BseLI	CCNNNNNNNGG	6 cut(s)	205, 413, 477, 851, 1660, 2295
BseMII	CTCAG	7 cut(s)	154, 1005, 1111, 1619, 2265, 2439, 2763
BseNI	ACTGG	5 cut(s)	1323, 2053, 2130, 2271, 2855
BseRI	GAGGAG	4 cut(s)	626, 950, 1518, 2301
BseSI	GKGCMC	2 cut(s)	536, 1986
BseX3I	CGGCCG	1 cut(s)	87
BseYI	CCCAGC	1 cut(s)	701
Bsh1236I	CGCG	3 cut(s)	169, 171, 270
Bsh1285I	CGRYCG	2 cut(s)	90, 199
BshFI	GGCC	4 cut(s)	89, 945, 2777, 2893
BshNI	GGYRCC	2 cut(s)	1519, 1983
BshTI	ACCGGT	1 cut(s)	1653
BsiEI	CGRYCG	2 cut(s)	90, 199
BsiHKAI	GWGCWC	2 cut(s)	1425, 1642
BsiHKCI	CYCGRG	1 cut(s)	1997
BsiWI	CGTACG	1 cut(s)	903
BslFI	GGGAC	3 cut(s)	578, 1169, 2111
BslI	CCNNNNNNNGG	6 cut(s)	205, 413, 477, 851, 1660, 2295
BsmAI	GTCTC	4 cut(s)	76, 977, 1530, 1998
BsmBI	CGTCTC	1 cut(s)	1530
BsmFI	GGGAC	3 cut(s)	578, 1169, 2111
BsmI	GAATGC	2 cut(s)	1279, 1403
BsnI	GGCC	4 cut(s)	89, 945, 2777, 2893
Bso31I	GGTCTC	2 cut(s)	76, 977
BsoBI	CYCGRG	1 cut(s)	1997
Bsp119I	TTCGAA	1 cut(s)	1977
Bsp1286I	GDGCHC	7 cut(s)	96, 536, 676, 1045, 1425, 1642, 1986
Bsp13I	TCCGGA	1 cut(s)	54
Bsp1407I	TGTACA	1 cut(s)	2583
Bsp19I	CCATGG	2 cut(s)	1764, 2778
BspANI	GGCC	4 cut(s)	89, 945, 2777, 2893
BspCNI	CTCAG	7 cut(s)	153, 1006, 1110, 1618, 2266, 2440, 2764
BspEI	TCCGGA	1 cut(s)	54
BspFNI	CGCG	3 cut(s)	169, 171, 270
BspHI	TCATGA	3 cut(s)	300, 1407, 1597
BspMAI	CTGCAG	1 cut(s)	2689
BspMI	ACCTGC	1 cut(s)	1954
BspQI	GCTCTTC	1 cut(s)	27
BspT104I	TTCGAA	1 cut(s)	1977
BspT107I	GGYRCC	2 cut(s)	1519, 1983
BspTI	CTTAAG	2 cut(s)	632, 2570
BspTNI	GGTCTC	2 cut(s)	76, 977
BsrBI	CCGCTC	1 cut(s)	483
BsrFI	RCCGGY	3 cut(s)	85, 1653, 2889
BsrGI	TGTACA	1 cut(s)	2583
BsrI	ACTGG	5 cut(s)	1323, 2053, 2130, 2271, 2855
BssAI	RCCGGY	3 cut(s)	85, 1653, 2889
BssECI	CCNNGG	7 cut(s)	228, 471, 539, 728, 1764, 2757, 2778
BssT1I	CCWWGG	3 cut(s)	1764, 2757, 2778
Bst2UI	CCWGG	2 cut(s)	68, 540
Bst4CI	ACNGT	9 cut(s)	200, 298, 932, 1193, 1524, 1992, 2884, 2942, 3021
Bst6I	CTCTTC	3 cut(s)	27, 1979, 2163
BstAFI	CTTAAG	2 cut(s)	632, 2570
BstAUI	TGTACA	1 cut(s)	2583
BstBI	TTCGAA	1 cut(s)	1977
BstC8I	GCNNGC	8 cut(s)	87, 152, 842, 1124, 1552, 1638, 1969, 3047
BstDSI	CCRYGG	4 cut(s)	228, 728, 1764, 2778
BstEII	GGTNACC	1 cut(s)	1187
BstFNI	CGCG	3 cut(s)	169, 171, 270
BstHHI	GCGC	2 cut(s)	171, 272
BstMAI	GTCTC	4 cut(s)	76, 977, 1530, 1998
BstMCI	CGRYCG	2 cut(s)	90, 199
BstNI	CCWGG	2 cut(s)	68, 540
BstNSI	RCATGY	5 cut(s)	1087, 1226, 1480, 2965, 3049
BstPAI	GACNNNNGTC	1 cut(s)	282
BstPI	GGTNACC	1 cut(s)	1187
BstSCI	CCNGG	5 cut(s)	66, 393, 406, 538, 1787
BstSFI	CTRYAG	2 cut(s)	2415, 2685
BstSLI	GKGCMC	2 cut(s)	536, 1986
BstUI	CGCG	3 cut(s)	169, 171, 270
BstV2I	GAAGAC	1 cut(s)	2718
BstX2I	RGATCY	3 cut(s)	378, 421, 2851
BstYI	RGATCY	3 cut(s)	378, 421, 2851
BstZI	CGGCCG	1 cut(s)	87
Bsu36I	CCTNAGG	1 cut(s)	2696
BsuI	GTATCC	1 cut(s)	1129
BsuRI	GGCC	4 cut(s)	89, 945, 2777, 2893
BtgI	CCRYGG	4 cut(s)	228, 728, 1764, 2778
BtsI	GCAGTG	1 cut(s)	866
BtsIMutI	CAGTG	5 cut(s)	536, 814, 866, 1105, 2046
BveI	ACCTGC	1 cut(s)	1954
Cac8I	GCNNGC	8 cut(s)	87, 152, 842, 1124, 1552, 1638, 1969, 3047
CaiI	CAGNNNCTG	1 cut(s)	1961
CciI	TCATGA	3 cut(s)	300, 1407, 1597
CfoI	GCGC	2 cut(s)	171, 272
Cfr10I	RCCGGY	3 cut(s)	85, 1653, 2889
Cfr13I	GGNCC	3 cut(s)	586, 791, 2504
CseI	GACGC	3 cut(s)	276, 423, 432
Csp6I	GTAC	6 cut(s)	247, 904, 1469, 1953, 2584, 2836
CspAI	ACCGGT	1 cut(s)	1653
CviQI	GTAC	6 cut(s)	247, 904, 1469, 1953, 2584, 2836
DraI	TTTAAA	1 cut(s)	1696
DrdI	GACNNNNNNGTC	2 cut(s)	2720, 2894
DseDI	GACNNNNNNGTC	2 cut(s)	2720, 2894
EaeI	YGGCCR	1 cut(s)	87
EagI	CGGCCG	1 cut(s)	87
Eam1104I	CTCTTC	3 cut(s)	27, 1979, 2163
EarI	CTCTTC	3 cut(s)	27, 1979, 2163
EciI	GGCGGA	4 cut(s)	146, 338, 2075, 2543
EclXI	CGGCCG	1 cut(s)	87
Eco130I	CCWWGG	3 cut(s)	1764, 2757, 2778
Eco24I	GRGCYC	3 cut(s)	96, 676, 1045
Eco31I	GGTCTC	2 cut(s)	76, 977
Eco47I	GGWCC	3 cut(s)	586, 791, 2504
Eco52I	CGGCCG	1 cut(s)	87
Eco57I	CTGAAG	2 cut(s)	1254, 1509
Eco81I	CCTNAGG	1 cut(s)	2696
Eco88I	CYCGRG	1 cut(s)	1997
Eco91I	GGTNACC	1 cut(s)	1187
EcoO109I	RGGNCCY	2 cut(s)	791, 2504
EcoO65I	GGTNACC	1 cut(s)	1187
EcoRI	GAATTC	1 cut(s)	2816
EcoRII	CCWGG	2 cut(s)	66, 538
EcoT14I	CCWWGG	3 cut(s)	1764, 2757, 2778
EcoT22I	ATGCAT	1 cut(s)	1228
EcoT38I	GRGCYC	3 cut(s)	96, 676, 1045
ErhI	CCWWGG	3 cut(s)	1764, 2757, 2778
Esp3I	CGTCTC	1 cut(s)	1530
FalI	AAGNNNNNCTT	4 cut(s)	1482, 1514, 2781, 2813
FaqI	GGGAC	3 cut(s)	578, 1169, 2111
FauI	CCCGC	1 cut(s)	2342
FbaI	TGATCA	1 cut(s)	2205
FblI	GTMKAC	5 cut(s)	5, 111, 2034, 2305, 2886
FriOI	GRGCYC	3 cut(s)	96, 676, 1045
FspBI	CTAG	7 cut(s)	251, 513, 780, 1466, 1673, 2160, 3081
GlaI	GCGC	2 cut(s)	170, 271
GsaI	CCCAGC	1 cut(s)	705
GsuI	CTGGAG	3 cut(s)	59, 1217, 2288
HaeIII	GGCC	4 cut(s)	89, 945, 2777, 2893
HgaI	GACGC	3 cut(s)	276, 423, 432
HhaI	GCGC	2 cut(s)	171, 272
Hin6I	GCGC	2 cut(s)	169, 270
HinP1I	GCGC	2 cut(s)	169, 270
HincII	GTYRAC	2 cut(s)	6, 1713
HindII	GTYRAC	2 cut(s)	6, 1713
HindIII	AAGCTT	1 cut(s)	356
HphI	GGTGA	8 cut(s)	286, 716, 730, 1199, 1295, 1935, 2621, 2720
Hpy99I	CGWCG	3 cut(s)	270, 281, 448
HpyAV	CCTTC	6 cut(s)	592, 1359, 1973, 2842, 2877, 2980
HpyCH4III	ACNGT	9 cut(s)	200, 298, 932, 1193, 1524, 1992, 2884, 2942, 3021
HpyCH4IV	ACGT	3 cut(s)	906, 1356, 2784
HpyCH4V	TGCA	8 cut(s)	980, 1073, 1226, 1480, 1733, 2494, 2687, 3049
HpySE526I	ACGT	3 cut(s)	906, 1356, 2784
HspAI	GCGC	2 cut(s)	169, 270
Kpn2I	TCCGGA	1 cut(s)	54
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	1 cut(s)	2205
LguI	GCTCTTC	1 cut(s)	27
LmnI	GCTCC	5 cut(s)	82, 232, 480, 613, 2437
LweI	GCATC	7 cut(s)	1235, 1471, 1742, 1750, 1782, 1911, 2917
MaeI	CTAG	7 cut(s)	251, 513, 780, 1466, 1673, 2160, 3081
MaeII	ACGT	3 cut(s)	906, 1356, 2784
MaeIII	GTNAC	4 cut(s)	260, 907, 1187, 1240
MbiI	CCGCTC	1 cut(s)	483
MfeI	CAATTG	2 cut(s)	1532, 2564
MflI	RGATCY	3 cut(s)	378, 421, 2851
MhlI	GDGCHC	7 cut(s)	96, 536, 676, 1045, 1425, 1642, 1986
MlyI	GAGTC	5 cut(s)	99, 191, 341, 560, 721
MmeI	TCCRAC	2 cut(s)	266, 1800
Mph1103I	ATGCAT	1 cut(s)	1228
MroI	TCCGGA	1 cut(s)	54
MroNI	GCCGGC	1 cut(s)	85
MroXI	GAANNNNTTC	2 cut(s)	62, 2793
MslI	CAYNNNNRTG	4 cut(s)	299, 807, 2139, 2843
MspA1I	CMGCKG	2 cut(s)	1958, 2633
MspCI	CTTAAG	2 cut(s)	632, 2570
MspR9I	CCNGG	5 cut(s)	68, 395, 408, 540, 1789
MunI	CAATTG	2 cut(s)	1532, 2564
Mva1269I	GAATGC	2 cut(s)	1279, 1403
MvaI	CCWGG	2 cut(s)	68, 540
MvnI	CGCG	3 cut(s)	169, 171, 270
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	3 cut(s)	395, 408, 1789
NcoI	CCATGG	2 cut(s)	1764, 2778
NgoMIV	GCCGGC	1 cut(s)	85
NmeAIII	GCCGAG	1 cut(s)	115
NmuCI	GTSAC	2 cut(s)	907, 1187
NsiI	ATGCAT	1 cut(s)	1228
NspI	RCATGY	5 cut(s)	1087, 1226, 1480, 2965, 3049
NspV	TTCGAA	1 cut(s)	1977
PaeI	GCATGC	1 cut(s)	3049
PaeR7I	CTCGAG	1 cut(s)	1997
PagI	TCATGA	3 cut(s)	300, 1407, 1597
PaqCI	CACCTGC	1 cut(s)	1954
PciI	ACATGT	1 cut(s)	2961
PciSI	GCTCTTC	1 cut(s)	27
PcsI	WCGNNNNNNNCGW	2 cut(s)	425, 3020
PctI	GAATGC	2 cut(s)	1279, 1403
PdiI	GCCGGC	1 cut(s)	87
PdmI	GAANNNNTTC	2 cut(s)	62, 2793
PfeI	GAWTC	7 cut(s)	136, 721, 736, 1812, 2014, 2248, 2877
Pfl23II	CGTACG	1 cut(s)	903
PflFI	GACNNNGTC	1 cut(s)	433
PfoI	TCCNGGA	1 cut(s)	1787
PinAI	ACCGGT	1 cut(s)	1653
PleI	GAGTC	5 cut(s)	99, 190, 340, 560, 720
PpsI	GAGTC	5 cut(s)	99, 190, 340, 560, 720
PpuMI	RGGWCCY	2 cut(s)	791, 2504
PscI	ACATGT	1 cut(s)	2961
PshAI	GACNNNNGTC	1 cut(s)	282
PsiI	TTATAA	1 cut(s)	1844
Psp5II	RGGWCCY	2 cut(s)	791, 2504
Psp6I	CCWGG	2 cut(s)	66, 538
PspEI	GGTNACC	1 cut(s)	1187
PspFI	CCCAGC	1 cut(s)	701
PspGI	CCWGG	2 cut(s)	66, 538
PspLI	CGTACG	1 cut(s)	903
PspPI	GGNCC	3 cut(s)	586, 791, 2504
PspPPI	RGGWCCY	2 cut(s)	791, 2504
PstI	CTGCAG	1 cut(s)	2689
PstNI	CAGNNNCTG	1 cut(s)	1961
PsuI	RGATCY	3 cut(s)	378, 421, 2851
PsyI	GACNNNGTC	1 cut(s)	433
PvuII	CAGCTG	2 cut(s)	1958, 2633
RsaI	GTAC	6 cut(s)	248, 905, 1470, 1954, 2585, 2837
RsaNI	GTAC	6 cut(s)	247, 904, 1469, 1953, 2584, 2836
RseI	CAYNNNNRTG	4 cut(s)	299, 807, 2139, 2843
SalI	GTCGAC	1 cut(s)	4
SapI	GCTCTTC	1 cut(s)	27
Sau96I	GGNCC	3 cut(s)	586, 791, 2504
SchI	GAGTC	5 cut(s)	99, 191, 341, 560, 721
ScrFI	CCNGG	5 cut(s)	68, 395, 408, 540, 1789
SduI	GDGCHC	7 cut(s)	96, 536, 676, 1045, 1425, 1642, 1986
SfaNI	GCATC	7 cut(s)	1235, 1471, 1742, 1750, 1782, 1911, 2917
SfcI	CTRYAG	2 cut(s)	2415, 2685
Sfr274I	CTCGAG	1 cut(s)	1997
SfuI	TTCGAA	1 cut(s)	1977
SinI	GGWCC	3 cut(s)	586, 791, 2504
SlaI	CTCGAG	1 cut(s)	1997
SmiMI	CAYNNNNRTG	4 cut(s)	299, 807, 2139, 2843
SmlI	CTYRAG	8 cut(s)	316, 632, 1001, 1556, 1997, 2313, 2349, 2570
SmoI	CTYRAG	8 cut(s)	316, 632, 1001, 1556, 1997, 2313, 2349, 2570
SpeI	ACTAGT	1 cut(s)	779
SphI	GCATGC	1 cut(s)	3049
SspI	AATATT	2 cut(s)	2095, 2359
SspMI	CTAG	7 cut(s)	251, 513, 780, 1466, 1673, 2160, 3081
StyD4I	CCNGG	5 cut(s)	66, 393, 406, 538, 1787
StyI	CCWWGG	3 cut(s)	1764, 2757, 2778
TaaI	ACNGT	9 cut(s)	200, 298, 932, 1193, 1524, 1992, 2884, 2942, 3021
TaiI	ACGT	3 cut(s)	909, 1359, 2787
TatI	WGTACW	3 cut(s)	1468, 2583, 2835
TauI	GCSGC	7 cut(s)	131, 221, 224, 227, 238, 486, 1371
TfiI	GAWTC	7 cut(s)	136, 721, 736, 1812, 2014, 2248, 2877
TscAI	CASTG	5 cut(s)	536, 814, 866, 1105, 2053
TseFI	GTSAC	2 cut(s)	907, 1187
Tsp45I	GTSAC	2 cut(s)	907, 1187
TspGWI	ACGGA	3 cut(s)	95, 325, 1631
TspRI	CASTG	5 cut(s)	536, 814, 866, 1105, 2053
Tth111I	GACNNNGTC	1 cut(s)	433
Vha464I	CTTAAG	2 cut(s)	632, 2570
VpaK11BI	GGWCC	3 cut(s)	586, 791, 2504
XapI	RAATTY	8 cut(s)	652, 874, 1141, 1727, 1826, 2188, 2197, 2816
XceI	RCATGY	5 cut(s)	1087, 1226, 1480, 2965, 3049
XhoI	CTCGAG	1 cut(s)	1997
XmiI	GTMKAC	5 cut(s)	5, 111, 2034, 2305, 2886
XmnI	GAANNNNTTC	2 cut(s)	62, 2793
XspI	CTAG	7 cut(s)	251, 513, 780, 1466, 1673, 2160, 3081
Zsp2I	ATGCAT	1 cut(s)	1228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.