MD09G1112700.v1.1

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004164
Physical Location & Seq
Reverse (-)
8564402 .. 8566243
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1112700.v1.1.491

Sequence Viewer

Length: 411 bp
ATGAGGAACAACACTTTGTGGACGCTCCTTGCTCGGAGAGCATCGACAACCACCACACTCCGAAACCCAACTTCAAGTCGGCGGTTCTTTTGCTCGCCACCGTCTTCCTCCTCCTCTCCACCCCAAAACAACAAGCTTTTCGTTGGAGGATTGTCATGGTCGGTGGATGAGAAGTCCTTAAAAGACGCTTTCTCATCCTTCGGGGAGGTCACTGACGTGAAGATAGTGTATGACAGAGATTCCGGCAGGTCGAGAGGGTTCGGATTTGTTAATTTCTCCAAGGAAGATGAAGCCCAGGTTGCAAAAGATGCCATGGATGGAAAGGCATTGTTGGGTCGGCCATTGAGGGTTACTTCTGCTGTTGAAAAAATTCGGGGCGGACCTGTTGTGGTTCCTCGCCTTCCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

137

Amino Acids

14.91

Weight (kDa)

10.26

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 46 - 116 1.2e-22 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AciI CCGC 2 cut(s) 82, 378
AcoI YGGCCR 1 cut(s) 338
AcsI RAATTY 1 cut(s) 369
AdeI CACNNNGTG 1 cut(s) 18
AgsI TTSAA 2 cut(s) 75, 365
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 294
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
AoxI GGCC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 380
AvaII GGWCC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 96
BccI CCATC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 237
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 380
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 50, 298
BpiI GAAGAC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 279, 294, 312
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 279, 294, 312
BseGI GGATG 3 cut(s) 172, 194, 322
BseRI GAGGAG 2 cut(s) 100, 103
BshFI GGCC 1 cut(s) 340
BsiSI CCGG 1 cut(s) 243
BsnI GGCC 1 cut(s) 340
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 2 cut(s) 82, 378
BspANI GGCC 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 393
BspMI ACCTGC 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 279, 294, 312
BssT1I CCWWGG 2 cut(s) 279, 312
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 172, 194, 322
BstMWI GCNNNNNNNGC 3 cut(s) 38, 299, 308
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 340
BtgI CCRYGG 1 cut(s) 312
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 172, 194, 322
BtsIMutI CAGTG 1 cut(s) 210
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 380
CseI GACGC 2 cut(s) 31, 194
CviAII CATG 3 cut(s) 156, 313, 408
CviJI RGCY 3 cut(s) 136, 293, 340
CviKI_1 RGCY 3 cut(s) 136, 293, 340
DraIII CACNNNGTG 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 338
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 2 cut(s) 279, 312
Eco47I GGWCC 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 279, 312
ErhI CCWWGG 2 cut(s) 279, 312
FaeI CATG 3 cut(s) 159, 316, 411
FaiI YATR 4 cut(s) 157, 231, 314, 409
FatI CATG 3 cut(s) 155, 312, 407
FokI GGATG 3 cut(s) 179, 181, 329
HaeIII GGCC 1 cut(s) 340
HapII CCGG 1 cut(s) 243
HgaI GACGC 2 cut(s) 31, 194
Hin1II CATG 3 cut(s) 159, 316, 411
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 243
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 3 cut(s) 36, 62, 263
Hpy188III TCNNGA 1 cut(s) 252
Hpy8I GTNNAC 1 cut(s) 21
HpyAV CCTTC 2 cut(s) 208, 410
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 299, 308
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 3 cut(s) 159, 316, 411
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 5 cut(s) 232, 256, 281, 308, 396
LweI GCATC 2 cut(s) 50, 298
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 2 cut(s) 208, 349
MboII GAAGA 3 cut(s) 96, 232, 296
MluCI AATT 2 cut(s) 271, 369
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 8 cut(s) 118, 121, 124, 140, 199, 248, 339, 405
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 2 cut(s) 179, 270
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 38, 299, 308
NcoI CCATGG 1 cut(s) 312
NlaIII CATG 3 cut(s) 159, 316, 411
NlaIV GGNNCC 1 cut(s) 393
NmuCI GTSAC 1 cut(s) 208
OliI CACNNNNGTG 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 1 cut(s) 380
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 179, 270
Sau96I GGNCC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 296
SetI ASST 6 cut(s) 138, 210, 219, 251, 300, 385
SfaNI GCATC 2 cut(s) 50, 298
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 2 cut(s) 271, 369
SsiI CCGC 2 cut(s) 82, 378
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 2 cut(s) 279, 312
TaaI ACNGT 1 cut(s) 102
TaiI ACGT 1 cut(s) 219
TaqI TCGA 2 cut(s) 44, 251
TasI AATT 2 cut(s) 271, 369
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 179, 270
Tru9I TTAA 2 cut(s) 179, 270
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 208
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 303
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.