Rw4G018740

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004164
Physical Location & Seq
Forward (+)
41543563 .. 41545477
1915 bp
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UTR
Exon/CDS
Intron
Rw4G018740.1

Sequence Viewer

Length: 426 bp
ATGCTGAACAACACTCTGTTAACGCTCCTTGCTAGAGGAGCATCAACAGGAGTAAATCAAAACCCAATTTTACTTCAGCGATGCTTCTGTTCACCACTGTCTTCTTCTTCAAACAATAAGCTCTTCGTCGGAGGGCCGGCTCTGTCATGGTCCTTGGACGAGAAGTCCCTAAAAGACGCTTTCTCTTCCTATGGGGAGGTCACTGAAGTGACTATAGTGTATGACAAGGACTCAGGCAGGTCTAGAGGCTTTGGATTTGTTAATTTCTCCAAGGACGATGATGCCAAGTCTGCAAAAGATGCTAAGGATGGAAAGGCGTTGATGGGTCGGCCACTGAGGATTAGTTTTGCTCTCGAAAGACTTCGGGGAGGGACTGTAGTTGTTCCTCGCCTTCCAGCCATTGGAGATGGTGTTAATCGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

141

Amino Acids

15.13

Weight (kDa)

9.47

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 48 - 113 4.4e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 329
AcuI CTGAAG 2 cut(s) 59, 225
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 1 cut(s) 111
AhdI GACNNNNNGTC 1 cut(s) 163
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
AoxI GGCC 2 cut(s) 134, 329
Asp700I GAANNNNTTC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 134, 150
AsuHPI GGTGA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 93
BccI CCATC 3 cut(s) 302, 316, 401
BfaI CTAG 2 cut(s) 33, 243
BfmI CTRYAG 2 cut(s) 213, 375
BfuAI ACCTGC 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 150
BmeRI GACNNNNNGTC 1 cut(s) 163
BmgT120I GGNCC 2 cut(s) 134, 150
BmsI GCATC 4 cut(s) 50, 71, 271, 289
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 153, 270
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 396, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 136
BseDI CCNNGG 2 cut(s) 153, 270
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 246, 326
BseRI GAGGAG 1 cut(s) 51
BshFI GGCC 2 cut(s) 136, 331
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 151, 385
BslI CCNNNNNNNGG 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 151, 385
BsnI GGCC 2 cut(s) 136, 331
BspANI GGCC 2 cut(s) 136, 331
BspCNI CTCAG 2 cut(s) 245, 327
BspMI ACCTGC 1 cut(s) 228
BspQI GCTCTTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 153, 270
BssT1I CCWWGG 2 cut(s) 153, 270
Bst4CI ACNGT 2 cut(s) 99, 376
Bst6I CTCTTC 2 cut(s) 128, 190
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 232, 303, 335
BstF5I GGATG 1 cut(s) 313
BstMWI GCNNNNNNNGC 3 cut(s) 38, 290, 299
BstSFI CTRYAG 2 cut(s) 213, 375
BstV2I GAAGAC 1 cut(s) 93
BsuRI GGCC 2 cut(s) 136, 331
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 3 cut(s) 95, 201, 332
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 134, 150
CseI GACGC 1 cut(s) 185
CviAII CATG 1 cut(s) 147
CviJI RGCY 6 cut(s) 121, 136, 140, 249, 331, 398
CviKI_1 RGCY 6 cut(s) 121, 136, 140, 249, 331, 398
DdeI CTNAG 3 cut(s) 232, 303, 335
DriI GACNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 329
Eam1104I CTCTTC 2 cut(s) 128, 190
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 2 cut(s) 128, 190
Eco130I CCWWGG 2 cut(s) 153, 270
Eco47I GGWCC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 59, 225
EcoT14I CCWWGG 2 cut(s) 153, 270
ErhI CCWWGG 2 cut(s) 153, 270
FaeI CATG 1 cut(s) 150
FaiI YATR 5 cut(s) 148, 192, 215, 222, 424
FaqI GGGAC 2 cut(s) 151, 385
FatI CATG 1 cut(s) 146
FokI GGATG 1 cut(s) 320
FspBI CTAG 2 cut(s) 33, 243
HaeIII GGCC 2 cut(s) 136, 331
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 150
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 1 cut(s) 230
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 84
Hpy166II GTNNAC 2 cut(s) 21, 92
Hpy188I TCNGA 1 cut(s) 131
Hpy188III TCNNGA 2 cut(s) 243, 353
Hpy8I GTNNAC 2 cut(s) 21, 92
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 2 cut(s) 99, 376
HpyCH4V TGCA 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 290, 299
HpyF3I CTNAG 3 cut(s) 232, 303, 335
Hsp92II CATG 1 cut(s) 150
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 21
LguI GCTCTTC 1 cut(s) 128
LmnI GCTCC 2 cut(s) 30, 38
LpnPI CCDG 5 cut(s) 33, 150, 219, 223, 408
LweI GCATC 4 cut(s) 50, 71, 271, 289
MaeI CTAG 2 cut(s) 33, 243
MaeIII GTNAC 2 cut(s) 199, 208
MboII GAAGA 5 cut(s) 93, 96, 99, 115, 177
MluCI AATT 2 cut(s) 66, 262
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 7 cut(s) 29, 125, 190, 239, 330, 362, 396
MroNI GCCGGC 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 20, 261, 414
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 38, 290, 299
NaeI GCCGGC 1 cut(s) 138
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 199, 208
OliI CACNNNNGTG 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 128
PdiI GCCGGC 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 360
PflMI CCANNNNNTGG 1 cut(s) 401
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PspPI GGNCC 2 cut(s) 134, 150
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 3 cut(s) 20, 261, 414
Sau96I GGNCC 2 cut(s) 134, 150
SchI GAGTC 1 cut(s) 224
SetI ASST 3 cut(s) 123, 201, 242
SfaNI GCATC 4 cut(s) 50, 71, 271, 289
SfcI CTRYAG 2 cut(s) 213, 375
SinI GGWCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 2 cut(s) 66, 262
SspMI CTAG 2 cut(s) 33, 243
StyI CCWWGG 2 cut(s) 153, 270
TaaI ACNGT 2 cut(s) 99, 376
TaqI TCGA 1 cut(s) 354
TasI AATT 2 cut(s) 66, 262
Tru1I TTAA 3 cut(s) 20, 261, 414
Tru9I TTAA 3 cut(s) 20, 261, 414
TscAI CASTG 3 cut(s) 102, 208, 339
TseFI GTSAC 2 cut(s) 199, 208
Tsp45I GTSAC 2 cut(s) 199, 208
TspRI CASTG 3 cut(s) 102, 208, 339
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 150
XbaI TCTAGA 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 33, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.