Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000968

Physical Location & Seq

Genomic Coordinates Forward (+)

10360604 .. 10364660

Gene Structure 4057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1134900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGATGACCACCATCCTCCGCCGTACGCCTACTATAGCCACGGCGCGGACTTTCGCCGCCATCGAAACCCTCCTGATCAACCACCATTCATTTCTCGGCGCATCGGCCCCCGAGCTCGTACTCCCCGCAAATGGTGCCGTCGCCGGTGAGGCTAGGTTATACACCGCCTCGGCACCGAGGACGAAGTTGGGTTCTTCGTACGGGTATCAAATAAGGGACTTGCACGCGAAGTCTGGGGCGTTGAGTTTTCAAGCGTCGACGGCTCTGGACGCGCAGCTCGCCGTTGATTACTCCTCCGACGAGTCGAGTAGAAGGAGCGGTGACGATGAAGGACTCGAGATCGCGAAGCTAGGGATTGCTAAGGAGATCGTGTCGGCTTTGGCCAATAAGGGTATCAACAAGCTGTTCCCCATACAGAGGGCTGTGTTGGAACCTGCGATGCAAGGGCAGGATATGATTGGTCGTGCTCGAACAGGAACAGGGAAAACTCTTGCGTTTGGGATACCCATTTTGGATAAAATCTTTAAACACAATGCCATAAAGGGGCGTGGAAAGAATCCTTTGGCTTTGGTTTTGGCCCCGACAAGAGAACTTGCAAAGCAAGTTGAGAAGGAATTCCATGATGCAGCACCTGGTTTGGAGACAATCTGTGTCTATGGTGGTACCCCCATTTCAAGTCAAATGAGAGCGCTTGACTATGGAGTTGATGTGGCTGTTGGCACGCCTGGTCGTGTAATTGATCTTATCAATAGGGGAGCCCTGAATTTGGGGGAAGTTCAGTTTATTGTTCTTGATGAAGCTGATCAGATGCTCCAGGTGGGATTCCAGGAGGCTGTAGAGACAATCTTAGAGAGGGTACCAAAGAAACGTCAGACGTTGTTGTTTTCTGCAACAATGCCCAATTGGATTAGAAAGCTTACTCAGAATTATCTAAAAGATCCGGCCGTTATTGATCTAGTTGGGGATTCTGATCAGAAATTGGCAGATGGAATTTCTCTATACTCAATTGTATCGGACTCATATGGAAGAGCATCAATTATCGGACCACTTATAACAGAACATGCAAAAGGAGGAAAGGCTATTGTTTTCACTCAGACAAAGCGTGATGCTGATCGATTGGCATATGCCATGTCAAGAAGCTTTAGATGTGAGGCTTTGCATGGGGATATTTCACAAAGCCAGAGGGAAAGGACCCTTGCTGGTTTTCGAAATGGGAATTTTAATATTTTAGTTGCCACTGACGTTGCTGCTCGTGGGCTTGATGTACCCAATGTTGATCTGGTGATACATTATGAGTTGCCAAATAATCCAGAAATATTTGTTCACCGGTCTGGCCGAACTGGTCGTGCTGGGAAGAAAGGAAGTGCTATTCTTGTTTACACACAGGCTGAATCCAGGGCTGTTAGGACTATTGAGCAAGAAGTAGGATGCAAATTTACTGAGCTCCCTCGGATTGCTGTTGACAGCAAAAGTATAGACATGATGCCTGACATGGGTAGAGATCGATCTGGCACGAAGGGTAGTCGATTTGGTGCTCCTTCAGGACCAGGTTTTGGCCGTCTTGGTGGGTTTGGTGGTTCCAATAGCTATGGTGATTCTTCTGGTCAGATGGGTAGATCTAGTGGGCCTCGTCCCGGTCGTTATGGTGCCTTCGGTGATTCTCAATCTGGAAGTGGGTTTTCCGGATCTAGTTCAAACCGCGGAGGAAACTTTGGTAATTCAGGTTTTGGTCGTTCAAGTGGTTTTGGTAGTTCTGACCGCTCAAGTGGTTTCGGTAGTTCTGACCGATCAAGTGGGTTCGGAGTGTTTGGTAGCTCTGATCGCTCTGGTGGTTCTAGGAACTTTGGTTCGGATCGCCAGGGTGGACTTGACAATAATTCTAGCCGTTTTGGGAGCTTTGGGAATGACAGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010467 GO:0010501 GO:0016032 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018995 GO:0019034 GO:0019058 GO:0019079 GO:0030312 GO:0031974 GO:0031981 GO:0033643 GO:0033646 GO:0033647 GO:0033648 GO:0034641 GO:0039694 GO:0042025 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043656 GO:0043657 GO:0044094 GO:0044215 GO:0044216 GO:0044217 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051704 GO:0070013 GO:0070035 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

639

Amino Acids

67.88

Weight (kDa)

9.44

Isoelectric Point (pI)

35.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	137 - 304	3.8e-46	DEAD/DEAH box helicase
Helicase_C	PF00271	350 - 451	2.1e-33	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22310 AT3G22310 AT3G22330
fragaria_vesca	FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140
malus_domestica	MD09G1134900.v1.1 MD17G1123900.v1.1
prunus_persica	Prupe.3G191800_v2.0.a1 Prupe.3G191800_v2.0.a1
pyrus_communis	pycom09g05600 pycom17g11530
rosa_chinensis	RchiOBHm_Chr2g0154771 RchiOBHm_Chr2g0154851 RchiOBHm_Chr2g0155131
rosa_laevigata	RLG00000020802 RLG00000020805 RLG00000020809 RLG00000020814 RLG00000020820
rosa_multiflora	Rmu_sc0001027.1_g000001 Rmu_sc0002048.1_g000007 Rmu_sc0002048.1_g000019 Rmu_sc0002048.1_g000035 Rmu_sc0004976.1_g000007
rosa_roxburghii	Rroxscaffold_2G00093940 Rroxscaffold_2G00094090
rosa_rugosa	Rorug02G0444900.1 Rorug02G0445000 Rorug02G0445500 Rorug02G0445600
rosa_samantha	Rh2AG510600 Rh2AG511000 Rh2AG511200 Rh2AG511700 Rh2BG521500 Rh2BG521700 Rh2BG522200 Rh2BG522500 Rh2CG495800 Rh2CG496100 Rh2CG496300 Rh2CG496700 Rh2DG531600 Rh2DG531900 Rh2DG532200
rosa_wichuraiana	Rw2G042000 Rw2G042030 Rw2G042040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1052
Acc36I	ACCTGC	1 cut(s)	442
Acc65I	GGTACC	2 cut(s)	662, 856
AccB1I	GGYRCC	5 cut(s)	134, 172, 662, 856, 1646
AccB7I	CCANNNNNTGG	1 cut(s)	1553
AccBSI	CCGCTC	2 cut(s)	318, 1761
AccI	GTMKAC	1 cut(s)	257
AccII	CGCG	5 cut(s)	46, 227, 272, 344, 1701
AccIII	TCCGGA	1 cut(s)	1682
AciI	CCGC	9 cut(s)	19, 46, 57, 126, 165, 318, 1699, 1701, 1759
AclWI	GGATC	3 cut(s)	932, 1693, 1860
AcoI	YGGCCR	4 cut(s)	381, 942, 1333, 1555
AcsI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
AcuI	CTGAAG	1 cut(s)	1524
AfaI	GTAC	6 cut(s)	25, 120, 200, 664, 858, 1266
AfeI	AGCGCT	1 cut(s)	690
AfiI	CCNNNNNNNGG	8 cut(s)	45, 131, 417, 543, 766, 1493, 1553, 1634
AgeI	ACCGGT	1 cut(s)	1326
AgsI	TTSAA	4 cut(s)	251, 675, 1695, 1737
AjnI	CCWGG	7 cut(s)	631, 724, 813, 825, 1394, 1546, 1857
AloI	GAACNNNNNNTCC	2 cut(s)	1831, 1863
Alw21I	GWGCWC	4 cut(s)	117, 469, 1446, 1537
Alw26I	GTCTC	2 cut(s)	635, 833
AlwI	GGATC	3 cut(s)	932, 1693, 1860
AlwNI	CAGNNNCTG	1 cut(s)	632
Ama87I	CYCGRG	2 cut(s)	110, 335
Aor13HI	TCCGGA	1 cut(s)	1682
Aor51HI	AGCGCT	1 cut(s)	690
AoxI	GGCC	7 cut(s)	105, 381, 576, 942, 1333, 1555, 1625
ApeKI	GCWGC	3 cut(s)	274, 626, 1247
ApoI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
AsiGI	ACCGGT	1 cut(s)	1326
Asp700I	GAANNNNTTC	1 cut(s)	614
Asp718I	GGTACC	2 cut(s)	662, 856
AspLEI	GCGC	4 cut(s)	46, 101, 274, 691
AspS9I	GGNCC	6 cut(s)	106, 577, 1043, 1191, 1544, 1625
AsuC2I	CCSGG	1 cut(s)	1635
AsuHPI	GGTGA	6 cut(s)	158, 332, 1294, 1316, 1604, 1667
AsuII	TTCGAA	1 cut(s)	1207
AvaI	CYCGRG	2 cut(s)	110, 335
AvaII	GGWCC	3 cut(s)	1043, 1191, 1544
BalI	TGGCCA	1 cut(s)	383
BanI	GGYRCC	5 cut(s)	134, 172, 662, 856, 1646
BanII	GRGCYC	3 cut(s)	117, 760, 1446
BauI	CACGAG	1 cut(s)	1251
Bbv12I	GWGCWC	4 cut(s)	117, 469, 1446, 1537
BbvI	GCAGC	3 cut(s)	286, 638, 1234
BccI	CCATC	4 cut(s)	20, 68, 980, 1603
BceAI	ACGGC	8 cut(s)	6, 57, 122, 266, 276, 929, 1542, 1869
BciT130I	CCWGG	7 cut(s)	633, 726, 815, 827, 1396, 1548, 1859
BciVI	GTATCC	1 cut(s)	495
BclI	TGATCA	3 cut(s)	75, 802, 970
BcnI	CCSGG	1 cut(s)	1635
BcoDI	GTCTC	2 cut(s)	635, 833
BfaI	CTAG	7 cut(s)	153, 350, 956, 1620, 1689, 1836, 1881
BfmI	CTRYAG	2 cut(s)	33, 834
BfoI	RGCGCY	1 cut(s)	692
BfuAI	ACCTGC	1 cut(s)	442
BfuI	GTATCC	1 cut(s)	495
BglI	GCCNNNNNGGC	1 cut(s)	149
BglII	AGATCT	1 cut(s)	1616
BisI	GCNGC	4 cut(s)	57, 275, 627, 1248
BlsI	GCNGC	4 cut(s)	58, 276, 628, 1249
Bme1390I	CCNGG	8 cut(s)	633, 726, 815, 827, 1396, 1548, 1635, 1859
Bme18I	GGWCC	3 cut(s)	1043, 1191, 1544
BmeT110I	CYCGRG	2 cut(s)	110, 335
BmgT120I	GGNCC	6 cut(s)	106, 577, 1043, 1191, 1544, 1625
BmrFI	CCNGG	8 cut(s)	633, 726, 815, 827, 1396, 1548, 1635, 1859
BmsI	GCATC	8 cut(s)	110, 429, 613, 798, 1040, 1096, 1418, 1473
BpmI	CTGGAG	1 cut(s)	797
Bpu10I	CCTNAGC	1 cut(s)	360
Bpu14I	TTCGAA	1 cut(s)	1207
BpuEI	CTTGAG	1 cut(s)	1747
BpuMI	CCSGG	1 cut(s)	1635
Bsa29I	ATCGAT	2 cut(s)	1114, 1504
BsaBI	GATNNNNATC	2 cut(s)	969, 1110
BsaJI	CCNNGG	7 cut(s)	39, 168, 176, 1395, 1448, 1699, 1858
BsaWI	WCCGGW	2 cut(s)	1326, 1682
BsaXI	ACNNNNNCTCC	4 cut(s)	356, 386, 693, 723
Bsc4I	CCNNNNNNNGG	8 cut(s)	45, 131, 417, 543, 766, 1493, 1553, 1634
Bse118I	RCCGGY	2 cut(s)	143, 1326
Bse1I	ACTGG	1 cut(s)	1345
Bse8I	GATNNNNATC	2 cut(s)	969, 1110
BseAI	TCCGGA	1 cut(s)	1682
BseBI	CCWGG	7 cut(s)	633, 726, 815, 827, 1396, 1548, 1859
BseCI	ATCGAT	2 cut(s)	1114, 1504
BseDI	CCNNGG	7 cut(s)	39, 168, 176, 1395, 1448, 1699, 1858
BseGI	GGATG	2 cut(s)	12, 1433
BseJI	GATNNNNATC	2 cut(s)	969, 1110
BseLI	CCNNNNNNNGG	8 cut(s)	45, 131, 417, 543, 766, 1493, 1553, 1634
BseMII	CTCAG	3 cut(s)	935, 1106, 1431
BseNI	ACTGG	1 cut(s)	1345
BseRI	GAGGAG	1 cut(s)	283
BseX3I	CGGCCG	1 cut(s)	942
BseXI	GCAGC	3 cut(s)	286, 638, 1234
BseYI	CCCAGC	1 cut(s)	1349
Bsh1236I	CGCG	5 cut(s)	46, 227, 272, 344, 1701
Bsh1285I	CGRYCG	2 cut(s)	945, 1639
BshFI	GGCC	7 cut(s)	107, 383, 578, 944, 1335, 1557, 1627
BshNI	GGYRCC	5 cut(s)	134, 172, 662, 856, 1646
BshTI	ACCGGT	1 cut(s)	1326
BshVI	ATCGAT	2 cut(s)	1114, 1504
BsiEI	CGRYCG	2 cut(s)	945, 1639
BsiHKAI	GWGCWC	4 cut(s)	117, 469, 1446, 1537
BsiHKCI	CYCGRG	2 cut(s)	110, 335
BsiSI	CCGG	5 cut(s)	144, 941, 1327, 1635, 1683
BsiWI	CGTACG	2 cut(s)	23, 198
BslFI	GGGAC	2 cut(s)	230, 1617
BslI	CCNNNNNNNGG	8 cut(s)	45, 131, 417, 543, 766, 1493, 1553, 1634
BsmAI	GTCTC	2 cut(s)	635, 833
BsmFI	GGGAC	2 cut(s)	230, 1617
BsnI	GGCC	7 cut(s)	107, 383, 578, 944, 1335, 1557, 1627
BsoBI	CYCGRG	2 cut(s)	110, 335
Bsp119I	TTCGAA	1 cut(s)	1207
Bsp1286I	GDGCHC	5 cut(s)	117, 469, 760, 1446, 1537
Bsp13I	TCCGGA	1 cut(s)	1682
Bsp68I	TCGCGA	1 cut(s)	344
BspACI	CCGC	9 cut(s)	19, 46, 57, 126, 165, 318, 1699, 1701, 1759
BspANI	GGCC	7 cut(s)	107, 383, 578, 944, 1335, 1557, 1627
BspCNI	CTCAG	3 cut(s)	934, 1105, 1432
BspDI	ATCGAT	2 cut(s)	1114, 1504
BspEI	TCCGGA	1 cut(s)	1682
BspFNI	CGCG	5 cut(s)	46, 227, 272, 344, 1701
BspMI	ACCTGC	1 cut(s)	442
BspPI	GGATC	3 cut(s)	932, 1693, 1860
BspQI	GCTCTTC	1 cut(s)	1021
BspT104I	TTCGAA	1 cut(s)	1207
BspT107I	GGYRCC	5 cut(s)	134, 172, 662, 856, 1646
BsrBI	CCGCTC	2 cut(s)	318, 1761
BsrFI	RCCGGY	2 cut(s)	143, 1326
BsrI	ACTGG	1 cut(s)	1345
BssAI	RCCGGY	2 cut(s)	143, 1326
BssECI	CCNNGG	7 cut(s)	39, 168, 176, 1395, 1448, 1699, 1858
BssSI	CACGAG	1 cut(s)	1251
Bst2BI	CACGAG	1 cut(s)	1251
Bst2UI	CCWGG	7 cut(s)	633, 726, 815, 827, 1396, 1548, 1859
Bst6I	CTCTTC	1 cut(s)	1021
BstAPI	GCANNNNNTGC	1 cut(s)	134
BstBI	TTCGAA	1 cut(s)	1207
BstC8I	GCNNGC	3 cut(s)	225, 279, 722
BstDEI	CTNAG	5 cut(s)	360, 847, 921, 1092, 1440
BstDSI	CCRYGG	2 cut(s)	39, 1699
BstF5I	GGATG	2 cut(s)	12, 1433
BstFNI	CGCG	5 cut(s)	46, 227, 272, 344, 1701
BstH2I	RGCGCY	1 cut(s)	692
BstHHI	GCGC	4 cut(s)	46, 101, 274, 691
BstMAI	GTCTC	2 cut(s)	635, 833
BstMCI	CGRYCG	2 cut(s)	945, 1639
BstMWI	GCNNNNNNNGC	6 cut(s)	134, 149, 260, 269, 278, 1821
BstNI	CCWGG	7 cut(s)	633, 726, 815, 827, 1396, 1548, 1859
BstNSI	RCATGY	1 cut(s)	1064
BstSCI	CCNGG	8 cut(s)	631, 724, 813, 825, 1394, 1546, 1633, 1857
BstSFI	CTRYAG	2 cut(s)	33, 834
BstUI	CGCG	5 cut(s)	46, 227, 272, 344, 1701
BstV1I	GCAGC	3 cut(s)	286, 638, 1234
BstX2I	RGATCY	3 cut(s)	937, 1616, 1685
BstYI	RGATCY	3 cut(s)	937, 1616, 1685
BstZI	CGGCCG	1 cut(s)	942
Bsu15I	ATCGAT	2 cut(s)	1114, 1504
BsuI	GTATCC	1 cut(s)	495
BsuRI	GGCC	7 cut(s)	107, 383, 578, 944, 1335, 1557, 1627
BsuTUI	ATCGAT	2 cut(s)	1114, 1504
BtgI	CCRYGG	2 cut(s)	39, 1699
BtgZI	GCGATG	1 cut(s)	452
BtsCI	GGATG	2 cut(s)	12, 1433
BtsIMutI	CAGTG	1 cut(s)	1236
BtuMI	TCGCGA	1 cut(s)	344
BveI	ACCTGC	1 cut(s)	442
Cac8I	GCNNGC	3 cut(s)	225, 279, 722
CaiI	CAGNNNCTG	1 cut(s)	632
CfoI	GCGC	4 cut(s)	46, 101, 274, 691
Cfr10I	RCCGGY	2 cut(s)	143, 1326
Cfr13I	GGNCC	6 cut(s)	106, 577, 1043, 1191, 1544, 1625
Cfr42I	CCGCGG	1 cut(s)	1702
ClaI	ATCGAT	2 cut(s)	1114, 1504
CseI	GACGC	2 cut(s)	243, 278
CsiI	ACCWGGT	2 cut(s)	631, 1546
Csp6I	GTAC	6 cut(s)	24, 119, 199, 663, 857, 1265
CspAI	ACCGGT	1 cut(s)	1326
CspCI	CAANNNNNGTGG	2 cut(s)	1225, 1260
CviAII	CATG	6 cut(s)	620, 1061, 1129, 1160, 1480, 1492
CviQI	GTAC	6 cut(s)	24, 119, 199, 663, 857, 1265
DdeI	CTNAG	5 cut(s)	360, 847, 921, 1092, 1440
DraI	TTTAAA	1 cut(s)	526
EaeI	YGGCCR	4 cut(s)	381, 942, 1333, 1555
EagI	CGGCCG	1 cut(s)	942
Eam1104I	CTCTTC	1 cut(s)	1021
EarI	CTCTTC	1 cut(s)	1021
EciI	GGCGGA	1 cut(s)	8
Ecl136II	GAGCTC	2 cut(s)	115, 1444
EclXI	CGGCCG	1 cut(s)	942
Eco24I	GRGCYC	3 cut(s)	117, 760, 1446
Eco47I	GGWCC	3 cut(s)	1043, 1191, 1544
Eco47III	AGCGCT	1 cut(s)	690
Eco52I	CGGCCG	1 cut(s)	942
Eco53kI	GAGCTC	2 cut(s)	115, 1444
Eco57I	CTGAAG	1 cut(s)	1524
Eco88I	CYCGRG	2 cut(s)	110, 335
EcoICRI	GAGCTC	2 cut(s)	115, 1444
EcoO109I	RGGNCCY	1 cut(s)	1191
EcoRI	GAATTC	1 cut(s)	614
EcoRII	CCWGG	7 cut(s)	631, 724, 813, 825, 1394, 1546, 1857
EcoT38I	GRGCYC	3 cut(s)	117, 760, 1446
FaeI	CATG	6 cut(s)	623, 1064, 1132, 1163, 1483, 1495
FaqI	GGGAC	2 cut(s)	230, 1617
FatI	CATG	6 cut(s)	619, 1060, 1128, 1159, 1479, 1491
FauI	CCCGC	1 cut(s)	133
FauNDI	CATATG	2 cut(s)	1021, 1123
FbaI	TGATCA	3 cut(s)	75, 802, 970
FblI	GTMKAC	1 cut(s)	257
Fnu4HI	GCNGC	4 cut(s)	57, 275, 627, 1248
FokI	GGATG	1 cut(s)	1440
FriOI	GRGCYC	3 cut(s)	117, 760, 1446
Fsp4HI	GCNGC	4 cut(s)	57, 275, 627, 1248
FspBI	CTAG	7 cut(s)	153, 350, 956, 1620, 1689, 1836, 1881
GlaI	GCGC	4 cut(s)	45, 100, 273, 690
GluI	GCNGC	4 cut(s)	57, 275, 627, 1248
GsaI	CCCAGC	1 cut(s)	1353
GsuI	CTGGAG	1 cut(s)	797
HaeII	RGCGCY	1 cut(s)	692
HaeIII	GGCC	7 cut(s)	107, 383, 578, 944, 1335, 1557, 1627
HapII	CCGG	5 cut(s)	144, 941, 1327, 1635, 1683
HgaI	GACGC	2 cut(s)	243, 278
HhaI	GCGC	4 cut(s)	46, 101, 274, 691
Hin1II	CATG	6 cut(s)	623, 1064, 1132, 1163, 1483, 1495
Hin6I	GCGC	4 cut(s)	44, 99, 272, 689
HinP1I	GCGC	4 cut(s)	44, 99, 272, 689
HincII	GTYRAC	2 cut(s)	258, 1462
HindII	GTYRAC	2 cut(s)	258, 1462
HindIII	AAGCTT	2 cut(s)	914, 1138
HinfI	GANTC	9 cut(s)	302, 333, 556, 822, 965, 1016, 1391, 1595, 1658
HpaII	CCGG	5 cut(s)	144, 941, 1327, 1635, 1683
HphI	GGTGA	6 cut(s)	158, 332, 1294, 1316, 1604, 1667
Hpy166II	GTNNAC	5 cut(s)	258, 1324, 1378, 1462, 1865
Hpy8I	GTNNAC	5 cut(s)	258, 1324, 1378, 1462, 1865
Hpy99I	CGWCG	4 cut(s)	143, 259, 262, 302
HpyAV	CCTTC	6 cut(s)	306, 323, 604, 1510, 1548, 1660
HpyCH4IV	ACGT	3 cut(s)	868, 875, 1242
HpyCH4V	TGCA	8 cut(s)	223, 442, 596, 626, 890, 1064, 1159, 1431
HpyF10VI	GCNNNNNNNGC	6 cut(s)	134, 149, 260, 269, 278, 1821
HpyF3I	CTNAG	5 cut(s)	360, 847, 921, 1092, 1440
HpySE526I	ACGT	3 cut(s)	868, 875, 1242
Hsp92II	CATG	6 cut(s)	623, 1064, 1132, 1163, 1483, 1495
HspAI	GCGC	4 cut(s)	44, 99, 272, 689
Kpn2I	TCCGGA	1 cut(s)	1682
KpnI	GGTACC	2 cut(s)	666, 860
Ksp22I	TGATCA	3 cut(s)	75, 802, 970
KspI	CCGCGG	1 cut(s)	1702
LguI	GCTCTTC	1 cut(s)	1021
LmnI	GCTCC	6 cut(s)	315, 755, 816, 1449, 1540, 1893
Lsp1109I	GCAGC	3 cut(s)	286, 638, 1234
LweI	GCATC	8 cut(s)	110, 429, 613, 798, 1040, 1096, 1418, 1473
MabI	ACCWGGT	2 cut(s)	631, 1546
MaeI	CTAG	7 cut(s)	153, 350, 956, 1620, 1689, 1836, 1881
MaeII	ACGT	3 cut(s)	868, 875, 1242
MaeIII	GTNAC	1 cut(s)	320
MbiI	CCGCTC	2 cut(s)	318, 1761
MboII	GAAGA	4 cut(s)	186, 1038, 1366, 1590
MfeI	CAATTG	2 cut(s)	901, 1005
MflI	RGATCY	3 cut(s)	937, 1616, 1685
MhlI	GDGCHC	5 cut(s)	117, 469, 760, 1446, 1537
MlsI	TGGCCA	1 cut(s)	383
MluNI	TGGCCA	1 cut(s)	383
MlyI	GAGTC	3 cut(s)	311, 327, 1010
MmeI	TCCRAC	2 cut(s)	321, 408
Mox20I	TGGCCA	1 cut(s)	383
MroI	TCCGGA	1 cut(s)	1682
MroXI	GAANNNNTTC	1 cut(s)	614
MscI	TGGCCA	1 cut(s)	383
MseI	TTAA	2 cut(s)	525, 1221
Msp20I	TGGCCA	1 cut(s)	383
MspA1I	CMGCKG	1 cut(s)	1701
MspI	CCGG	5 cut(s)	144, 941, 1327, 1635, 1683
MspR9I	CCNGG	8 cut(s)	633, 726, 815, 827, 1396, 1548, 1635, 1859
MunI	CAATTG	2 cut(s)	901, 1005
MvaI	CCWGG	7 cut(s)	633, 726, 815, 827, 1396, 1548, 1859
MvnI	CGCG	5 cut(s)	46, 227, 272, 344, 1701
MwoI	GCNNNNNNNGC	6 cut(s)	134, 149, 260, 269, 278, 1821
NciI	CCSGG	1 cut(s)	1635
NdeI	CATATG	2 cut(s)	1021, 1123
NlaIII	CATG	6 cut(s)	623, 1064, 1132, 1163, 1483, 1495
NmeAIII	GCCGAG	2 cut(s)	75, 149
NmuCI	GTSAC	1 cut(s)	320
NruI	TCGCGA	1 cut(s)	344
NspI	RCATGY	1 cut(s)	1064
NspV	TTCGAA	1 cut(s)	1207
PaeR7I	CTCGAG	1 cut(s)	335
PciSI	GCTCTTC	1 cut(s)	1021
PcsI	WCGNNNNNNNCGW	2 cut(s)	60, 1636
PdmI	GAANNNNTTC	1 cut(s)	614
PfeI	GAWTC	6 cut(s)	556, 822, 965, 1391, 1595, 1658
Pfl23II	CGTACG	2 cut(s)	23, 198
PflMI	CCANNNNNTGG	1 cut(s)	1553
PfoI	TCCNGGA	1 cut(s)	825
PinAI	ACCGGT	1 cut(s)	1326
PkrI	GCNGC	4 cut(s)	58, 276, 628, 1249
PleI	GAGTC	3 cut(s)	310, 327, 1010
PpsI	GAGTC	3 cut(s)	310, 327, 1010
PpuMI	RGGWCCY	1 cut(s)	1191
PsiI	TTATAA	1 cut(s)	1052
Psp124BI	GAGCTC	2 cut(s)	117, 1446
Psp5II	RGGWCCY	1 cut(s)	1191
Psp6I	CCWGG	7 cut(s)	631, 724, 813, 825, 1394, 1546, 1857
PspFI	CCCAGC	1 cut(s)	1349
PspGI	CCWGG	7 cut(s)	631, 724, 813, 825, 1394, 1546, 1857
PspLI	CGTACG	2 cut(s)	23, 198
PspPI	GGNCC	6 cut(s)	106, 577, 1043, 1191, 1544, 1625
PspPPI	RGGWCCY	1 cut(s)	1191
PstNI	CAGNNNCTG	1 cut(s)	632
PsuI	RGATCY	3 cut(s)	937, 1616, 1685
RruI	TCGCGA	1 cut(s)	344
RsaI	GTAC	6 cut(s)	25, 120, 200, 664, 858, 1266
RsaNI	GTAC	6 cut(s)	24, 119, 199, 663, 857, 1265
SacI	GAGCTC	2 cut(s)	117, 1446
SacII	CCGCGG	1 cut(s)	1702
SalI	GTCGAC	1 cut(s)	256
SapI	GCTCTTC	1 cut(s)	1021
SaqAI	TTAA	2 cut(s)	525, 1221
SatI	GCNGC	4 cut(s)	57, 275, 627, 1248
Sau96I	GGNCC	6 cut(s)	106, 577, 1043, 1191, 1544, 1625
SchI	GAGTC	3 cut(s)	311, 327, 1010
ScrFI	CCNGG	8 cut(s)	633, 726, 815, 827, 1396, 1548, 1635, 1859
SduI	GDGCHC	5 cut(s)	117, 469, 760, 1446, 1537
SexAI	ACCWGGT	2 cut(s)	631, 1546
SfaNI	GCATC	8 cut(s)	110, 429, 613, 798, 1040, 1096, 1418, 1473
SfcI	CTRYAG	2 cut(s)	33, 834
Sfr274I	CTCGAG	1 cut(s)	335
Sfr303I	CCGCGG	1 cut(s)	1702
SfuI	TTCGAA	1 cut(s)	1207
SgrAI	CRCCGGYG	1 cut(s)	143
SgrBI	CCGCGG	1 cut(s)	1702
SgrDI	CGTCGACG	1 cut(s)	256
SinI	GGWCC	3 cut(s)	1043, 1191, 1544
SlaI	CTCGAG	1 cut(s)	335
SmlI	CTYRAG	2 cut(s)	335, 1762
SmoI	CTYRAG	2 cut(s)	335, 1762
SsiI	CCGC	9 cut(s)	19, 46, 57, 126, 165, 318, 1699, 1701, 1759
SspI	AATATT	2 cut(s)	1225, 1317
SspMI	CTAG	7 cut(s)	153, 350, 956, 1620, 1689, 1836, 1881
SstI	GAGCTC	2 cut(s)	117, 1446
StyD4I	CCNGG	8 cut(s)	631, 724, 813, 825, 1394, 1546, 1633, 1857
TaiI	ACGT	3 cut(s)	871, 878, 1245
TaqI	TCGA	9 cut(s)	63, 257, 305, 336, 469, 1114, 1207, 1504, 1525
TaqII	GACCGA	1 cut(s)	1800
TauI	GCSGC	1 cut(s)	59
TfiI	GAWTC	6 cut(s)	556, 822, 965, 1391, 1595, 1658
Tru1I	TTAA	2 cut(s)	525, 1221
Tru9I	TTAA	2 cut(s)	525, 1221
TscAI	CASTG	1 cut(s)	1243
TseFI	GTSAC	1 cut(s)	320
TseI	GCWGC	3 cut(s)	274, 626, 1247
Tsp45I	GTSAC	1 cut(s)	320
TspDTI	ATGAA	3 cut(s)	78, 342, 810
TspRI	CASTG	1 cut(s)	1243
Van91I	CCANNNNNTGG	1 cut(s)	1553
VpaK11BI	GGWCC	3 cut(s)	1043, 1191, 1544
XapI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
XceI	RCATGY	1 cut(s)	1064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1276
XhoI	CTCGAG	1 cut(s)	335
XmiI	GTMKAC	1 cut(s)	257
XmnI	GAANNNNTTC	1 cut(s)	614
XspI	CTAG	7 cut(s)	153, 350, 956, 1620, 1689, 1836, 1881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.