Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000968

Physical Location & Seq

Genomic Coordinates Reverse (-)

68221486 .. 68224740

Gene Structure 3255 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G042000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGATGAGCATCATGCTCCGACGGACACCCTCGATGGCGCGCAACCTCGCCGCCATCGAAACCCTCCTCATAAACCGCCTCTCCTTCCCGCCAACGCCGTCATCCGGCCAAGTGATTCCGGCCTCAGGCGACGTCGTCGGCGAGGCCAAGACCTCCCACTGGTTGCAAGTTAGGAACTTTCAATCGAAATCGGTGCCGTTGAATTATCGCGCATCGCCGACTTTGTTGAGCGCCGCCCAGTTCGCAGTTGAGGACGGCCAATACGACGAGTCGTCTAAAAAGAGCGGCGACGATGGGCTCGAGATTTCGAAGCTAGGGATTGATCAGGAGATTGTATCTGCTTTGGCCAAGAAGGGGATCACTAAGCTTTTTCCCATTCAGAGAGCTGTGTTGGAACCAGCAATGCAAGGGCGTGATATGATTGGTCGTGCTAGAACTGGAACTGGGAAAACTCTTGCATTTGGGATTCCCATTTTGGATAAAATCATTCAATTCAATGCCAAACATGGCCGTGGGAGGAATCCTTTGGCTTTGGTGTTGGCTCCGACGAGAGAACTTGCAAAGCAAGTTGACAAGGAATTCTATGAGGCAGCACCGAGTTTGGATACGATTTGTGTGTATGGTGGTACACCCATTGGAAGGCAGATGGGCCAGCTTGACCATGGTGTTGACGTGGCGGTTGGCACACCTGGTCGTGTTATTGATCTCCTTAAGAGGGGTGCTTTGAATCTATCGGAAGTTCAGTTTGTTGTTCTTGATGAAGCTGACCAGATGCTTCAAGTGGGATTTCAGGAGGATGTGGAGATAATCTTGGAGAGATTACCAAAGAAACGTCAGACCTTGATGTTCTCTGCAACCATGCCTACATGGATCAAAAATCTTACTAGGAATTACCTAAAAGATCCAGCTGTTATCGATCTTGTTGGGGATTCTGATCAGAAATTGCCAGATGGAATTTCCCTATACTCGATTGCTGCAGACTCATATGGAAAAGCGTCAATTATTGGACCACTGATAACAGAACATGCAAGAGGAGGAAAGTGCATTGTTTTTACTCAGACGAAACGTGATGCTGATCGATTGGCATCTGCCATGGCAAGAAAGCATAATTGTGAGGCTTTGCATGGGGATATTTCACAACAACAGAGAGAAAGGACACTTGCAGGTTTTCGAGATGGGCGTTTCAATATTTTAGTTGCCACTGATGTTGCCGCTCGTGGGCTTGACGTACCCAATGTTGATCTGGTGATACATTACGAGCTCCCAAATAATTCAGAAATATTTGTTCATCGGTCAGGCAGAACAGGTCGTGCTGGGAAGAAAGGCAGTGCTATTCTTATTTACTCACAGGATCAAACCAGGAATGTAAGGATGATTGAGCGAGATGTTGGATGCCGCTTTAAAGAGCTTCCCCACATTGCTGTTGACAGCGGAAGTGCCGACATGTTCGACATAGGAAGTGGTGGTCGATTTAATCGTTTTGGTGGTCCAGGGGGAGGGGGACCTGGATTTGGCCGTTCTGGACCTGGCAGTAGATATAGTGGACCTAATTCAGGTCGTTTTGGTTCCTTTGGTGATTCTGGTCAATCTAGCACAAGTCGAGGTGGAAACTTTGGCAATTCAGGTTTCGGTCGCTCAAGTGGTTTTGGGAGTTCCGACCGTTCAAGTGGTTTCGGAAGTTTTGATCGTTCAGGTGGTTTCGGAAGTTCCGATCGTTCAAGCGGTTCTGACCGTTCAGGTGGTTTTGGAGGAGGGTTTGGTAGTTCCGACCGATCAGGTGGTTTTGGAGGATTTGGCGGTTCTGATCGTTCTAGTGGATTCAGAGATCGTTCCGGTGGTTCTAGGAACTATGGTTTAGATCGCCAGAGTGGATTTGATGACAATAATTCTAGCAGTTTTGGAGATGATCAGGATGCAGGAAGACGAGCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010467 GO:0010501 GO:0016032 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018995 GO:0019034 GO:0019058 GO:0019079 GO:0030312 GO:0031974 GO:0031981 GO:0033643 GO:0033646 GO:0033647 GO:0033648 GO:0034641 GO:0039694 GO:0042025 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043656 GO:0043657 GO:0044094 GO:0044215 GO:0044216 GO:0044217 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051704 GO:0070013 GO:0070035 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

644

Amino Acids

68.85

Weight (kDa)

9.31

Isoelectric Point (pI)

42.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	125 - 293	5.1e-46	DEAD/DEAH box helicase
Helicase_C	PF00271	333 - 439	5.3e-33	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22310 AT3G22310 AT3G22330
fragaria_vesca	FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140
malus_domestica	MD09G1134900.v1.1 MD17G1123900.v1.1
prunus_persica	Prupe.3G191800_v2.0.a1 Prupe.3G191800_v2.0.a1
pyrus_communis	pycom09g05600 pycom17g11530
rosa_chinensis	RchiOBHm_Chr2g0154771 RchiOBHm_Chr2g0154851 RchiOBHm_Chr2g0155131
rosa_laevigata	RLG00000020802 RLG00000020805 RLG00000020809 RLG00000020814 RLG00000020820
rosa_multiflora	Rmu_sc0001027.1_g000001 Rmu_sc0002048.1_g000007 Rmu_sc0002048.1_g000019 Rmu_sc0002048.1_g000035 Rmu_sc0004976.1_g000007
rosa_roxburghii	Rroxscaffold_2G00093940 Rroxscaffold_2G00094090
rosa_rugosa	Rorug02G0444900.1 Rorug02G0445000 Rorug02G0445500 Rorug02G0445600
rosa_samantha	Rh2AG510600 Rh2AG511000 Rh2AG511200 Rh2AG511700 Rh2BG521500 Rh2BG521700 Rh2BG522200 Rh2BG522500 Rh2CG495800 Rh2CG496100 Rh2CG496300 Rh2CG496700 Rh2DG531600 Rh2DG531900 Rh2DG532200
rosa_wichuraiana	Rw2G042000 Rw2G042030 Rw2G042040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	135
Acc36I	ACCTGC	1 cut(s)	1154
AccB1I	GGYRCC	1 cut(s)	193
AccBSI	CCGCTC	2 cut(s)	285, 1214
AccII	CGCG	2 cut(s)	40, 210
AclWI	GGATC	4 cut(s)	365, 878, 896, 1359
AcoI	YGGCCR	5 cut(s)	106, 256, 345, 508, 1513
AcsI	RAATTY	2 cut(s)	578, 954
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	2 cut(s)	628, 1230
AflII	CTTAAG	1 cut(s)	710
AflIII	ACRYGT	1 cut(s)	1443
AgsI	TTSAA	9 cut(s)	182, 202, 491, 496, 727, 779, 1186, 1665, 1719
AhdI	GACNNNNNGTC	1 cut(s)	271
AjiI	CACGTC	1 cut(s)	673
AjnI	CCWGG	5 cut(s)	688, 1358, 1489, 1504, 1525
AluBI	AGCT	8 cut(s)	313, 367, 386, 655, 764, 908, 1261, 1408
AluI	AGCT	8 cut(s)	313, 367, 386, 655, 764, 908, 1261, 1408
Alw21I	GWGCWC	1 cut(s)	1263
AlwI	GGATC	4 cut(s)	365, 878, 896, 1359
Ama87I	CYCGRG	1 cut(s)	299
AoxI	GGCC	8 cut(s)	106, 120, 144, 256, 345, 508, 649, 1513
ApeKI	GCWGC	2 cut(s)	590, 974
ApoI	RAATTY	2 cut(s)	578, 954
ArsI	GACNNNNNNTTYG	1 cut(s)	1914
AspLEI	GCGC	4 cut(s)	40, 42, 212, 233
AspS9I	GGNCC	6 cut(s)	649, 1007, 1487, 1502, 1523, 1544
AsuHPI	GGTGA	2 cut(s)	1258, 1586
AsuII	TTCGAA	1 cut(s)	308
AvaI	CYCGRG	1 cut(s)	299
AvaII	GGWCC	5 cut(s)	1007, 1487, 1502, 1523, 1544
AxyI	CCTNAGG	1 cut(s)	124
BalI	TGGCCA	1 cut(s)	347
BanI	GGYRCC	1 cut(s)	193
BanII	GRGCYC	2 cut(s)	300, 1263
BauI	CACGAG	1 cut(s)	1215
BbsI	GAAGAC	1 cut(s)	1927
Bbv12I	GWGCWC	1 cut(s)	1263
BbvI	GCAGC	2 cut(s)	602, 961
BccI	CCATC	6 cut(s)	28, 62, 287, 640, 944, 1169
BceAI	ACGGC	5 cut(s)	82, 181, 271, 495, 1500
BcgI	CGANNNNNNTGC	2 cut(s)	175, 209
BciT130I	CCWGG	5 cut(s)	690, 1360, 1491, 1506, 1527
BciVI	GTATCC	1 cut(s)	598
BclI	TGATCA	3 cut(s)	322, 934, 1906
BfaI	CTAG	7 cut(s)	314, 432, 885, 1590, 1812, 1842, 1890
BfmI	CTRYAG	1 cut(s)	975
BfoI	RGCGCY	1 cut(s)	234
BfrI	CTTAAG	1 cut(s)	710
BfuAI	ACCTGC	1 cut(s)	1154
BfuI	GTATCC	1 cut(s)	598
BisI	GCNGC	7 cut(s)	51, 234, 286, 591, 975, 1212, 1396
BlsI	GCNGC	7 cut(s)	52, 235, 287, 592, 976, 1213, 1397
Bme1390I	CCNGG	5 cut(s)	690, 1360, 1491, 1506, 1527
Bme18I	GGWCC	5 cut(s)	1007, 1487, 1502, 1523, 1544
BmeRI	GACNNNNNGTC	1 cut(s)	271
BmeT110I	CYCGRG	1 cut(s)	299
BmgBI	CACGTC	1 cut(s)	673
BmgT120I	GGNCC	6 cut(s)	649, 1007, 1487, 1502, 1523, 1544
BmiI	GGNNCC	5 cut(s)	195, 396, 543, 1503, 1567
BmrFI	CCNGG	5 cut(s)	690, 1360, 1491, 1506, 1527
BmrI	ACTGGG	2 cut(s)	232, 453
BmsI	GCATC	7 cut(s)	18, 221, 762, 1060, 1094, 1382, 1903
BmuI	ACTGGG	2 cut(s)	232, 453
BpiI	GAAGAC	1 cut(s)	1927
Bpu14I	TTCGAA	1 cut(s)	308
BpuEI	CTTGAG	1 cut(s)	1621
Bsa29I	ATCGAT	2 cut(s)	915, 1078
BsaBI	GATNNNNATC	4 cut(s)	8, 933, 1074, 1084
BsaHI	GRCGYC	1 cut(s)	132
BsaJI	CCNNGG	4 cut(s)	511, 661, 1092, 1490
BsaWI	WCCGGW	1 cut(s)	1832
BsaXI	ACNNNNNCTCC	2 cut(s)	65, 95
Bse1I	ACTGG	4 cut(s)	164, 238, 442, 448
Bse21I	CCTNAGG	1 cut(s)	124
Bse3DI	GCAATG	2 cut(s)	408, 1416
Bse8I	GATNNNNATC	4 cut(s)	8, 933, 1074, 1084
BseBI	CCWGG	5 cut(s)	690, 1360, 1491, 1506, 1527
BseCI	ATCGAT	2 cut(s)	915, 1078
BseDI	CCNNGG	4 cut(s)	511, 661, 1092, 1490
BseGI	GGATG	5 cut(s)	101, 802, 1377, 1397, 1918
BseJI	GATNNNNATC	4 cut(s)	8, 933, 1074, 1084
BseMI	GCAATG	2 cut(s)	408, 1416
BseMII	CTCAG	2 cut(s)	138, 1070
BseNI	ACTGG	4 cut(s)	164, 238, 442, 448
BsePI	GCGCGC	1 cut(s)	38
BseRI	GAGGAG	3 cut(s)	56, 1047, 1764
BseXI	GCAGC	2 cut(s)	602, 961
BseYI	CCCAGC	1 cut(s)	1313
Bsh1236I	CGCG	2 cut(s)	40, 210
Bsh1285I	CGRYCG	4 cut(s)	1633, 1660, 1714, 1771
BshFI	GGCC	8 cut(s)	108, 122, 146, 258, 347, 510, 651, 1515
BshNI	GGYRCC	1 cut(s)	193
BshVI	ATCGAT	2 cut(s)	915, 1078
BsiEI	CGRYCG	4 cut(s)	1633, 1660, 1714, 1771
BsiHKAI	GWGCWC	1 cut(s)	1263
BsiHKCI	CYCGRG	1 cut(s)	299
BsiSI	CCGG	3 cut(s)	105, 119, 1833
BslFI	GGGAC	1 cut(s)	1515
BsmFI	GGGAC	1 cut(s)	1515
BsnI	GGCC	8 cut(s)	108, 122, 146, 258, 347, 510, 651, 1515
BsoBI	CYCGRG	1 cut(s)	299
Bsp119I	TTCGAA	1 cut(s)	308
Bsp1286I	GDGCHC	2 cut(s)	300, 1263
Bsp19I	CCATGG	2 cut(s)	661, 1092
BspANI	GGCC	8 cut(s)	108, 122, 146, 258, 347, 510, 651, 1515
BspCNI	CTCAG	2 cut(s)	137, 1069
BspDI	ATCGAT	2 cut(s)	915, 1078
BspFNI	CGCG	2 cut(s)	40, 210
BspLI	GGNNCC	5 cut(s)	195, 396, 543, 1503, 1567
BspMAI	CTGCAG	1 cut(s)	979
BspMI	ACCTGC	1 cut(s)	1154
BspPI	GGATC	4 cut(s)	365, 878, 896, 1359
BspT104I	TTCGAA	1 cut(s)	308
BspT107I	GGYRCC	1 cut(s)	193
BspTI	CTTAAG	1 cut(s)	710
BsrBI	CCGCTC	2 cut(s)	285, 1214
BsrDI	GCAATG	2 cut(s)	408, 1416
BsrI	ACTGG	4 cut(s)	164, 238, 442, 448
BssECI	CCNNGG	4 cut(s)	511, 661, 1092, 1490
BssHII	GCGCGC	1 cut(s)	38
BssNI	GRCGYC	1 cut(s)	132
BssSI	CACGAG	1 cut(s)	1215
BssT1I	CCWWGG	2 cut(s)	661, 1092
Bst2BI	CACGAG	1 cut(s)	1215
Bst2UI	CCWGG	5 cut(s)	690, 1360, 1491, 1506, 1527
Bst4CI	ACNGT	2 cut(s)	1661, 1733
BstACI	GRCGYC	1 cut(s)	132
BstAFI	CTTAAG	1 cut(s)	710
BstBI	TTCGAA	1 cut(s)	308
BstC8I	GCNNGC	2 cut(s)	40, 653
BstDEI	CTNAG	3 cut(s)	124, 363, 1056
BstDSI	CCRYGG	3 cut(s)	511, 661, 1092
BstENI	CCTNNNNNAGG	1 cut(s)	1551
BstF5I	GGATG	5 cut(s)	101, 802, 1377, 1397, 1918
BstFNI	CGCG	2 cut(s)	40, 210
BstH2I	RGCGCY	1 cut(s)	234
BstHHI	GCGC	4 cut(s)	40, 42, 212, 233
BstMCI	CGRYCG	4 cut(s)	1633, 1660, 1714, 1771
BstMWI	GCNNNNNNNGC	1 cut(s)	242
BstNI	CCWGG	5 cut(s)	690, 1360, 1491, 1506, 1527
BstNSI	RCATGY	2 cut(s)	1028, 1447
BstSCI	CCNGG	5 cut(s)	688, 1358, 1489, 1504, 1525
BstSFI	CTRYAG	1 cut(s)	975
BstUI	CGCG	2 cut(s)	40, 210
BstV1I	GCAGC	2 cut(s)	602, 961
BstV2I	GAAGAC	1 cut(s)	1927
BstX2I	RGATCY	1 cut(s)	901
BstYI	RGATCY	1 cut(s)	901
Bsu15I	ATCGAT	2 cut(s)	915, 1078
Bsu36I	CCTNAGG	1 cut(s)	124
BsuI	GTATCC	1 cut(s)	598
BsuRI	GGCC	8 cut(s)	108, 122, 146, 258, 347, 510, 651, 1515
BsuTUI	ATCGAT	2 cut(s)	915, 1078
BtgI	CCRYGG	3 cut(s)	511, 661, 1092
BtgZI	GCGATG	1 cut(s)	198
BtrI	CACGTC	1 cut(s)	673
BtsCI	GGATG	5 cut(s)	101, 802, 1377, 1397, 1918
BtsI	GCAGTG	1 cut(s)	1333
BtsIMutI	CAGTG	4 cut(s)	157, 1010, 1200, 1333
BveI	ACCTGC	1 cut(s)	1154
Cac8I	GCNNGC	2 cut(s)	40, 653
CfoI	GCGC	4 cut(s)	40, 42, 212, 233
Cfr13I	GGNCC	6 cut(s)	649, 1007, 1487, 1502, 1523, 1544
ClaI	ATCGAT	2 cut(s)	915, 1078
CseI	GACGC	1 cut(s)	984
CsiI	ACCWGGT	1 cut(s)	688
Csp6I	GTAC	2 cut(s)	627, 1229
CspCI	CAANNNNNGTGG	2 cut(s)	1189, 1224
CviAII	CATG	9 cut(s)	13, 506, 662, 859, 867, 1025, 1093, 1124, 1444
CviQI	GTAC	2 cut(s)	627, 1229
DdeI	CTNAG	3 cut(s)	124, 363, 1056
DraI	TTTAAA	1 cut(s)	1402
DriI	GACNNNNNGTC	1 cut(s)	271
EaeI	YGGCCR	5 cut(s)	106, 256, 345, 508, 1513
Eam1105I	GACNNNNNGTC	1 cut(s)	271
Ecl136II	GAGCTC	1 cut(s)	1261
Eco130I	CCWWGG	2 cut(s)	661, 1092
Eco24I	GRGCYC	2 cut(s)	300, 1263
Eco47I	GGWCC	5 cut(s)	1007, 1487, 1502, 1523, 1544
Eco53kI	GAGCTC	1 cut(s)	1261
Eco81I	CCTNAGG	1 cut(s)	124
Eco88I	CYCGRG	1 cut(s)	299
EcoICRI	GAGCTC	1 cut(s)	1261
EcoNI	CCTNNNNNAGG	1 cut(s)	1551
EcoO109I	RGGNCCY	1 cut(s)	1502
EcoRI	GAATTC	1 cut(s)	578
EcoRII	CCWGG	5 cut(s)	688, 1358, 1489, 1504, 1525
EcoT14I	CCWWGG	2 cut(s)	661, 1092
EcoT38I	GRGCYC	2 cut(s)	300, 1263
ErhI	CCWWGG	2 cut(s)	661, 1092
FaeI	CATG	9 cut(s)	16, 509, 665, 862, 870, 1028, 1096, 1127, 1447
FaqI	GGGAC	1 cut(s)	1515
FatI	CATG	9 cut(s)	12, 505, 661, 858, 866, 1024, 1092, 1123, 1443
FauI	CCCGC	1 cut(s)	96
FauNDI	CATATG	1 cut(s)	985
FbaI	TGATCA	3 cut(s)	322, 934, 1906
Fnu4HI	GCNGC	7 cut(s)	51, 234, 286, 591, 975, 1212, 1396
FokI	GGATG	5 cut(s)	88, 809, 1384, 1404, 1925
FriOI	GRGCYC	2 cut(s)	300, 1263
Fsp4HI	GCNGC	7 cut(s)	51, 234, 286, 591, 975, 1212, 1396
FspBI	CTAG	7 cut(s)	314, 432, 885, 1590, 1812, 1842, 1890
GlaI	GCGC	4 cut(s)	39, 41, 211, 232
GluI	GCNGC	7 cut(s)	51, 234, 286, 591, 975, 1212, 1396
GsaI	CCCAGC	1 cut(s)	1317
HaeII	RGCGCY	1 cut(s)	234
HaeIII	GGCC	8 cut(s)	108, 122, 146, 258, 347, 510, 651, 1515
HapII	CCGG	3 cut(s)	105, 119, 1833
HgaI	GACGC	1 cut(s)	984
HhaI	GCGC	4 cut(s)	40, 42, 212, 233
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	9 cut(s)	16, 509, 665, 862, 870, 1028, 1096, 1127, 1447
Hin6I	GCGC	4 cut(s)	38, 40, 210, 231
HinP1I	GCGC	4 cut(s)	38, 40, 210, 231
HincII	GTYRAC	3 cut(s)	571, 670, 1426
HindII	GTYRAC	3 cut(s)	571, 670, 1426
HindIII	AAGCTT	1 cut(s)	365
HinfI	GANTC	9 cut(s)	115, 269, 466, 520, 727, 929, 980, 1577, 1818
HpaII	CCGG	3 cut(s)	105, 119, 1833
HphI	GGTGA	2 cut(s)	1258, 1586
Hpy166II	GTNNAC	5 cut(s)	571, 629, 670, 1426, 1544
Hpy188III	TCNNGA	7 cut(s)	301, 326, 755, 791, 1172, 1521, 1910
Hpy8I	GTNNAC	5 cut(s)	571, 629, 670, 1426, 1544
Hpy99I	CGWCG	7 cut(s)	24, 134, 137, 140, 269, 293, 550
HpyAV	CCTTC	3 cut(s)	94, 346, 633
HpyCH4III	ACNGT	2 cut(s)	1661, 1733
HpyCH4IV	ACGT	5 cut(s)	132, 672, 832, 1066, 1227
HpyF10VI	GCNNNNNNNGC	1 cut(s)	242
HpyF3I	CTNAG	3 cut(s)	124, 363, 1056
HpySE526I	ACGT	5 cut(s)	132, 672, 832, 1066, 1227
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	9 cut(s)	16, 509, 665, 862, 870, 1028, 1096, 1127, 1447
HspAI	GCGC	4 cut(s)	38, 40, 210, 231
Ksp22I	TGATCA	3 cut(s)	322, 934, 1906
LmnI	GCTCC	3 cut(s)	21, 547, 1266
Lsp1109I	GCAGC	2 cut(s)	602, 961
LweI	GCATC	7 cut(s)	18, 221, 762, 1060, 1094, 1382, 1903
MabI	ACCWGGT	1 cut(s)	688
MaeI	CTAG	7 cut(s)	314, 432, 885, 1590, 1812, 1842, 1890
MaeII	ACGT	5 cut(s)	132, 672, 832, 1066, 1227
MbiI	CCGCTC	2 cut(s)	285, 1214
MboII	GAAGA	2 cut(s)	1330, 1932
MflI	RGATCY	1 cut(s)	901
MhlI	GDGCHC	2 cut(s)	300, 1263
MlsI	TGGCCA	1 cut(s)	347
MluNI	TGGCCA	1 cut(s)	347
MlyI	GAGTC	2 cut(s)	278, 974
MmeI	TCCRAC	6 cut(s)	43, 372, 569, 1369, 1680, 1791
Mox20I	TGGCCA	1 cut(s)	347
MscI	TGGCCA	1 cut(s)	347
MseI	TTAA	3 cut(s)	711, 1401, 1473
MslI	CAYNNNNRTG	2 cut(s)	510, 1110
Msp20I	TGGCCA	1 cut(s)	347
MspA1I	CMGCKG	2 cut(s)	908, 1431
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	3 cut(s)	105, 119, 1833
MspR9I	CCNGG	5 cut(s)	690, 1360, 1491, 1506, 1527
MvaI	CCWGG	5 cut(s)	690, 1360, 1491, 1506, 1527
MvnI	CGCG	2 cut(s)	40, 210
MwoI	GCNNNNNNNGC	1 cut(s)	242
NcoI	CCATGG	2 cut(s)	661, 1092
NdeI	CATATG	1 cut(s)	985
NlaIII	CATG	9 cut(s)	16, 509, 665, 862, 870, 1028, 1096, 1127, 1447
NlaIV	GGNNCC	5 cut(s)	195, 396, 543, 1503, 1567
NspI	RCATGY	2 cut(s)	1028, 1447
NspV	TTCGAA	1 cut(s)	308
PaeR7I	CTCGAG	1 cut(s)	299
PauI	GCGCGC	1 cut(s)	38
PciI	ACATGT	1 cut(s)	1443
PcsI	WCGNNNNNNNCGW	7 cut(s)	54, 138, 261, 297, 1177, 1474, 1707
PfeI	GAWTC	7 cut(s)	115, 466, 520, 727, 929, 1577, 1818
PflFI	GACNNNGTC	1 cut(s)	134
PkrI	GCNGC	7 cut(s)	52, 235, 287, 592, 976, 1213, 1397
Ple19I	CGATCG	1 cut(s)	1714
PleI	GAGTC	2 cut(s)	277, 974
PpsI	GAGTC	2 cut(s)	277, 974
PpuMI	RGGWCCY	1 cut(s)	1502
PscI	ACATGT	1 cut(s)	1443
Psp124BI	GAGCTC	1 cut(s)	1263
Psp5II	RGGWCCY	1 cut(s)	1502
Psp6I	CCWGG	5 cut(s)	688, 1358, 1489, 1504, 1525
PspFI	CCCAGC	1 cut(s)	1313
PspGI	CCWGG	5 cut(s)	688, 1358, 1489, 1504, 1525
PspN4I	GGNNCC	5 cut(s)	195, 396, 543, 1503, 1567
PspPI	GGNCC	6 cut(s)	649, 1007, 1487, 1502, 1523, 1544
PspPPI	RGGWCCY	1 cut(s)	1502
PstI	CTGCAG	1 cut(s)	979
PsuI	RGATCY	1 cut(s)	901
PsyI	GACNNNGTC	1 cut(s)	134
PteI	GCGCGC	1 cut(s)	38
PvuI	CGATCG	1 cut(s)	1714
PvuII	CAGCTG	1 cut(s)	908
RsaI	GTAC	2 cut(s)	628, 1230
RsaNI	GTAC	2 cut(s)	627, 1229
RseI	CAYNNNNRTG	2 cut(s)	510, 1110
SacI	GAGCTC	1 cut(s)	1263
SaqAI	TTAA	3 cut(s)	711, 1401, 1473
SatI	GCNGC	7 cut(s)	51, 234, 286, 591, 975, 1212, 1396
Sau96I	GGNCC	6 cut(s)	649, 1007, 1487, 1502, 1523, 1544
SchI	GAGTC	2 cut(s)	278, 974
ScrFI	CCNGG	5 cut(s)	690, 1360, 1491, 1506, 1527
SduI	GDGCHC	2 cut(s)	300, 1263
SexAI	ACCWGGT	1 cut(s)	688
SfaNI	GCATC	7 cut(s)	18, 221, 762, 1060, 1094, 1382, 1903
SfcI	CTRYAG	1 cut(s)	975
Sfr274I	CTCGAG	1 cut(s)	299
SfuI	TTCGAA	1 cut(s)	308
SinI	GGWCC	5 cut(s)	1007, 1487, 1502, 1523, 1544
SlaI	CTCGAG	1 cut(s)	299
SmiMI	CAYNNNNRTG	2 cut(s)	510, 1110
SmlI	CTYRAG	3 cut(s)	299, 710, 1636
SmoI	CTYRAG	3 cut(s)	299, 710, 1636
SspI	AATATT	2 cut(s)	1189, 1281
SspMI	CTAG	7 cut(s)	314, 432, 885, 1590, 1812, 1842, 1890
SstI	GAGCTC	1 cut(s)	1263
StyD4I	CCNGG	5 cut(s)	688, 1358, 1489, 1504, 1525
StyI	CCWWGG	2 cut(s)	661, 1092
TaaI	ACNGT	2 cut(s)	1661, 1733
TaiI	ACGT	5 cut(s)	135, 675, 835, 1069, 1230
TaqII	GACCGA	3 cut(s)	1281, 1619, 1785
TauI	GCSGC	5 cut(s)	53, 236, 288, 1214, 1398
TfiI	GAWTC	7 cut(s)	115, 466, 520, 727, 929, 1577, 1818
Tru1I	TTAA	3 cut(s)	711, 1401, 1473
Tru9I	TTAA	3 cut(s)	711, 1401, 1473
TscAI	CASTG	4 cut(s)	164, 1017, 1207, 1333
TseI	GCWGC	2 cut(s)	590, 974
TspDTI	ATGAA	2 cut(s)	774, 1277
TspGWI	ACGGA	1 cut(s)	37
TspRI	CASTG	4 cut(s)	164, 1017, 1207, 1333
Tth111I	GACNNNGTC	1 cut(s)	134
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	5 cut(s)	1007, 1487, 1502, 1523, 1544
XagI	CCTNNNNNAGG	1 cut(s)	1551
XapI	RAATTY	2 cut(s)	578, 954
XceI	RCATGY	2 cut(s)	1028, 1447
XcmI	CCANNNNNNNNNTGG	2 cut(s)	659, 1240
XhoI	CTCGAG	1 cut(s)	299
XspI	CTAG	7 cut(s)	314, 432, 885, 1590, 1812, 1842, 1890
ZraI	GACGTC	1 cut(s)	133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.