Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000968

Physical Location & Seq

Genomic Coordinates Forward (+)

10764372 .. 10768465

Gene Structure 4094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1123900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGATGACCACCATCCTCCGCCGTACGCCTACTATAGCCACGGCGCGGACCTTTGCCGCCATCGAAACCTTCCTGATCAACCACCATTCATTTCTCGGGACGCCGGCCCCCGAACTCGTGCTTCCTGAAAATGGCGCCGTCGGCGGCGAGGCTAGGTTCTACACCGTCTCAGCCCTGAAGACGAAGTTGGGCCCTTCGTACGGGTATCAAGTGAGGGACTTGCACGCGAAATCTGGGCCGTTGAGTTTTCGGGCGTCGACGCCTCTGGGCGCGCAGTTTGCCGTTGAGTACTCATCCGAAGAGTCGACCAGAAGGAGTGGCGACGATGAAGGACTCGAGATCGCGAAGCTAGGGATTGCTAAGGAGATCGTGTCGGCTTTGGCCAATAAGGGTATCAGCAAGCTGTTCCCCATACAGAGGGCTGTGTTAGAACCAGCAATGCAAGGGCGGGATATGATCGGTCGCGCTCGAACAGGAACAGGGAAAACTCTTGCGTTTGGGATACCCATCTTGGATAAAATCTTTAAATACAATGCCATACATGGGCGTGGAAAGAATCCTTTGGCTTTGGTTTTGGCCCCGACAAGAGAACTCGCAAAGCAAGTTGAGAAGGAATTCCATGATACAGCACCTGGTTTGGATACAATCTGTGTCTATGGTGGTACCCCCATTTCAAGTCAAATGAGAGCACTTGACTATGGAGTTGATGTGGCTGTTGGCACGCCTGGTCGTGTAATTGATCTTATCAACAGGGGAGCCCTGAATTTAGAGGAAGTTCAGTTTGTTGTTCTTGATGAAGCTGATCAGATGCTCCAGGTGGGATTCCAGGAGGATGTAGAGATAATCTTAGAGAGGGTGCCAAAGAAACGTCAGACATTGTTGTTTTCTGCAACAATGCCCGGTTGGATTAGAAAGCTTACTCAGAATTATCTAAAAGATCCAGCCGTTATTGATCTTGTTGGGGATTCTGATCAAAAATTGGCAGATGGAATTTCTCTATACTCGATTGCATCAGACTCATACGGAAGAGCATCAATTATTGGACCACTTATAACAGAACATGCAAAAGGAGGAAAGACTATTGTTTTCACTCAGACAAAGCGTGATGCTGATCGATTGTCGTATGCCATGTCAAGAAGCTTTAGATGCGAGGCTTTGCATGGGGATATTTCACAAAGCCAGAGGGAAAGGACCCTTGCAGGTTTTCGAGATGGGAATTTCAATATTTTAGTTGCCACTGATGTTGCTGCTCGTGGGCTTGATGTACCCAATGTTGATCTGGTGATACATTATGAGCTGCCAAATAATCCAGAAATATTTGTTCACCGGTCTGGCCGAACAGGTCGTGCTGGGAAGAAAGGAAGGGCTATTCTTGTTTACACACAGGATCAATCCAGGGCTGTTAGGACTATTGAGAGAGAAGTAGGATGCAAATTTACGGAGCTTCCCCGGATTGCTGTTGACAGCAAAAGTATAGACATGATGCCGGACATGGGTAGAGATCGATTCACAAAGGGTAGTCGATTTGGTGGTCCATCAGGACCAGGTTTTAGCCGTTCTGGTGGGTTTGGTCGTTCCAGTAGCTATGGTGATTCTTCTGGTCAGATGGGTAGATCTAGTGGACCTCGTCCCGGTCGTTTTGGTTCCTATGGTGATTCTCAATCTGGAGGTGGGTTTTCCGGATCTAGCTCAAACCGCGGAGGAAACTTTGGTAACTCAGGTTTCGGTCGTTCAAGTGGTTTCGGTAATTCTGACCGTTCAGGTGGTTTTGGTAGTTCTGACCGTTCAAGTAATTTCGGAGGGTTTGGTAGCTCTGATCGTTCTGGTGGTTCTAGGAACAATAATTCTAGCCGTTTTGGCAGCTTTGGGGATGACAAGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0010467 GO:0010501 GO:0016032 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018995 GO:0019034 GO:0019058 GO:0019079 GO:0030312 GO:0031974 GO:0031981 GO:0033643 GO:0033646 GO:0033647 GO:0033648 GO:0034641 GO:0039694 GO:0042025 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043656 GO:0043657 GO:0044094 GO:0044215 GO:0044216 GO:0044217 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051704 GO:0070013 GO:0070035 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

628

Amino Acids

67.42

Weight (kDa)

9.47

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	137 - 303	5.4e-46	DEAD/DEAH box helicase
Helicase_C	PF00271	350 - 451	1.7e-33	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22310 AT3G22310 AT3G22330
fragaria_vesca	FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140 FvH4_6g40140
malus_domestica	MD09G1134900.v1.1 MD17G1123900.v1.1
prunus_persica	Prupe.3G191800_v2.0.a1 Prupe.3G191800_v2.0.a1
pyrus_communis	pycom09g05600 pycom17g11530
rosa_chinensis	RchiOBHm_Chr2g0154771 RchiOBHm_Chr2g0154851 RchiOBHm_Chr2g0155131
rosa_laevigata	RLG00000020802 RLG00000020805 RLG00000020809 RLG00000020814 RLG00000020820
rosa_multiflora	Rmu_sc0001027.1_g000001 Rmu_sc0002048.1_g000007 Rmu_sc0002048.1_g000019 Rmu_sc0002048.1_g000035 Rmu_sc0004976.1_g000007
rosa_roxburghii	Rroxscaffold_2G00093940 Rroxscaffold_2G00094090
rosa_rugosa	Rorug02G0444900.1 Rorug02G0445000 Rorug02G0445500 Rorug02G0445600
rosa_samantha	Rh2AG510600 Rh2AG511000 Rh2AG511200 Rh2AG511700 Rh2BG521500 Rh2BG521700 Rh2BG522200 Rh2BG522500 Rh2CG495800 Rh2CG496100 Rh2CG496300 Rh2CG496700 Rh2DG531600 Rh2DG531900 Rh2DG532200
rosa_wichuraiana	Rw2G042000 Rw2G042030 Rw2G042040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1052
Acc36I	ACCTGC	1 cut(s)	1190
Acc65I	GGTACC	1 cut(s)	662
AccB1I	GGYRCC	3 cut(s)	134, 662, 856
AccI	GTMKAC	2 cut(s)	257, 305
AccII	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
AccIII	TCCGGA	1 cut(s)	1679
AciI	CCGC	7 cut(s)	19, 46, 57, 144, 448, 1696, 1698
AclWI	GGATC	3 cut(s)	932, 1395, 1690
AcoI	YGGCCR	2 cut(s)	381, 1333
AcsI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
AcuI	CTGAAG	1 cut(s)	197
AcyI	GRCGYC	4 cut(s)	101, 135, 254, 260
AfaI	GTAC	5 cut(s)	25, 200, 290, 664, 1266
AfiI	CCNNNNNNNGG	7 cut(s)	45, 131, 200, 417, 543, 1493, 1631
AgeI	ACCGGT	1 cut(s)	1326
AgsI	TTSAA	4 cut(s)	675, 1222, 1734, 1788
AjnI	CCWGG	6 cut(s)	631, 724, 813, 825, 1394, 1543
AjuI	GAANNNNNNNTTGG	2 cut(s)	170, 202
Alw21I	GWGCWC	1 cut(s)	691
Alw26I	GTCTC	1 cut(s)	172
AlwI	GGATC	3 cut(s)	932, 1395, 1690
AlwNI	CAGNNNCTG	1 cut(s)	632
Ama87I	CYCGRG	2 cut(s)	95, 335
Aor13HI	TCCGGA	1 cut(s)	1679
AoxI	GGCC	6 cut(s)	105, 190, 236, 381, 576, 1333
ApaI	GGGCCC	1 cut(s)	194
ApeKI	GCWGC	3 cut(s)	1247, 1297, 1860
ApoI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
AsiGI	ACCGGT	1 cut(s)	1326
Asp700I	GAANNNNTTC	1 cut(s)	614
Asp718I	GGTACC	1 cut(s)	662
AspLEI	GCGC	5 cut(s)	46, 137, 272, 274, 467
AsuC2I	CCSGG	3 cut(s)	900, 1450, 1632
AsuHPI	GGTGA	4 cut(s)	1294, 1316, 1601, 1664
AvaI	CYCGRG	2 cut(s)	95, 335
AvaII	GGWCC	6 cut(s)	48, 1043, 1191, 1532, 1541, 1622
BaeGI	GKGCMC	1 cut(s)	194
BalI	TGGCCA	1 cut(s)	383
BanI	GGYRCC	3 cut(s)	134, 662, 856
BanII	GRGCYC	2 cut(s)	194, 760
BauI	CACGAG	2 cut(s)	116, 1251
BbsI	GAAGAC	1 cut(s)	185
Bbv12I	GWGCWC	1 cut(s)	691
BbvI	GCAGC	3 cut(s)	1234, 1284, 1872
BccI	CCATC	7 cut(s)	20, 68, 515, 980, 1205, 1543, 1600
BceAI	ACGGC	8 cut(s)	6, 57, 122, 223, 266, 929, 1539, 1836
BciT130I	CCWGG	6 cut(s)	633, 726, 815, 827, 1396, 1545
BciVI	GTATCC	2 cut(s)	495, 634
BclI	TGATCA	3 cut(s)	75, 802, 970
BcnI	CCSGG	3 cut(s)	900, 1450, 1632
BcoDI	GTCTC	1 cut(s)	172
BfaI	CTAG	6 cut(s)	153, 350, 1617, 1686, 1833, 1848
BfmI	CTRYAG	1 cut(s)	33
BfoI	RGCGCY	1 cut(s)	138
BfuAI	ACCTGC	1 cut(s)	1190
BfuI	GTATCC	2 cut(s)	495, 634
BglI	GCCNNNNNGGC	1 cut(s)	1857
BglII	AGATCT	1 cut(s)	1613
BisI	GCNGC	5 cut(s)	57, 145, 1248, 1298, 1861
BlsI	GCNGC	5 cut(s)	58, 146, 1249, 1299, 1862
BmcAI	AGTACT	1 cut(s)	290
Bme1390I	CCNGG	9 cut(s)	633, 726, 815, 827, 900, 1396, 1450, 1545, 1632
Bme18I	GGWCC	6 cut(s)	48, 1043, 1191, 1532, 1541, 1622
BmeT110I	CYCGRG	2 cut(s)	95, 335
BmiI	GGNNCC	9 cut(s)	108, 136, 192, 579, 664, 757, 858, 1193, 1645
BmrFI	CCNGG	9 cut(s)	633, 726, 815, 827, 900, 1396, 1450, 1545, 1632
BmsI	GCATC	7 cut(s)	798, 1019, 1040, 1096, 1136, 1418, 1473
BpiI	GAAGAC	1 cut(s)	185
BpmI	CTGGAG	2 cut(s)	797, 1686
Bpu10I	CCTNAGC	1 cut(s)	360
BpuMI	CCSGG	3 cut(s)	900, 1450, 1632
Bsa29I	ATCGAT	2 cut(s)	1114, 1504
BsaBI	GATNNNNATC	3 cut(s)	506, 969, 1110
BsaHI	GRCGYC	4 cut(s)	101, 135, 254, 260
BsaJI	CCNNGG	4 cut(s)	39, 1395, 1448, 1696
BsaWI	WCCGGW	2 cut(s)	1326, 1679
BsaXI	ACNNNNNCTCC	4 cut(s)	356, 386, 693, 723
Bsc4I	CCNNNNNNNGG	7 cut(s)	45, 131, 200, 417, 543, 1493, 1631
Bse118I	RCCGGY	2 cut(s)	103, 1326
Bse1I	ACTGG	1 cut(s)	1578
Bse3DI	GCAATG	1 cut(s)	444
Bse8I	GATNNNNATC	3 cut(s)	506, 969, 1110
BseAI	TCCGGA	1 cut(s)	1679
BseBI	CCWGG	6 cut(s)	633, 726, 815, 827, 1396, 1545
BseCI	ATCGAT	2 cut(s)	1114, 1504
BseDI	CCNNGG	4 cut(s)	39, 1395, 1448, 1696
BseGI	GGATG	5 cut(s)	12, 293, 838, 1433, 1876
BseJI	GATNNNNATC	3 cut(s)	506, 969, 1110
BseLI	CCNNNNNNNGG	7 cut(s)	45, 131, 200, 417, 543, 1493, 1631
BseMI	GCAATG	1 cut(s)	444
BseMII	CTCAG	4 cut(s)	183, 935, 1106, 1731
BseNI	ACTGG	1 cut(s)	1578
BsePI	GCGCGC	1 cut(s)	270
BseSI	GKGCMC	1 cut(s)	194
BseXI	GCAGC	3 cut(s)	1234, 1284, 1872
BseYI	CCCAGC	1 cut(s)	1349
Bsh1236I	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
Bsh1285I	CGRYCG	3 cut(s)	463, 1636, 1729
BshFI	GGCC	6 cut(s)	107, 192, 238, 383, 578, 1335
BshNI	GGYRCC	3 cut(s)	134, 662, 856
BshTI	ACCGGT	1 cut(s)	1326
BshVI	ATCGAT	2 cut(s)	1114, 1504
BsiEI	CGRYCG	3 cut(s)	463, 1636, 1729
BsiHKAI	GWGCWC	1 cut(s)	691
BsiHKCI	CYCGRG	2 cut(s)	95, 335
BsiSI	CCGG	7 cut(s)	104, 900, 1327, 1450, 1487, 1632, 1680
BsiWI	CGTACG	2 cut(s)	23, 198
BslFI	GGGAC	3 cut(s)	112, 230, 1614
BslI	CCNNNNNNNGG	7 cut(s)	45, 131, 200, 417, 543, 1493, 1631
BsmAI	GTCTC	1 cut(s)	172
BsmBI	CGTCTC	1 cut(s)	172
BsmFI	GGGAC	3 cut(s)	112, 230, 1614
BsnI	GGCC	6 cut(s)	107, 192, 238, 383, 578, 1335
BsoBI	CYCGRG	2 cut(s)	95, 335
Bsp120I	GGGCCC	1 cut(s)	190
Bsp1286I	GDGCHC	3 cut(s)	194, 691, 760
Bsp13I	TCCGGA	1 cut(s)	1679
Bsp68I	TCGCGA	1 cut(s)	344
BspACI	CCGC	7 cut(s)	19, 46, 57, 144, 448, 1696, 1698
BspANI	GGCC	6 cut(s)	107, 192, 238, 383, 578, 1335
BspCNI	CTCAG	4 cut(s)	182, 934, 1105, 1730
BspDI	ATCGAT	2 cut(s)	1114, 1504
BspEI	TCCGGA	1 cut(s)	1679
BspFNI	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
BspLI	GGNNCC	9 cut(s)	108, 136, 192, 579, 664, 757, 858, 1193, 1645
BspMI	ACCTGC	1 cut(s)	1190
BspPI	GGATC	3 cut(s)	932, 1395, 1690
BspQI	GCTCTTC	1 cut(s)	1021
BspT107I	GGYRCC	3 cut(s)	134, 662, 856
BsrDI	GCAATG	1 cut(s)	444
BsrFI	RCCGGY	2 cut(s)	103, 1326
BsrI	ACTGG	1 cut(s)	1578
BssAI	RCCGGY	2 cut(s)	103, 1326
BssECI	CCNNGG	4 cut(s)	39, 1395, 1448, 1696
BssHII	GCGCGC	1 cut(s)	270
BssNI	GRCGYC	4 cut(s)	101, 135, 254, 260
BssSI	CACGAG	2 cut(s)	116, 1251
Bst2BI	CACGAG	2 cut(s)	116, 1251
Bst2UI	CCWGG	6 cut(s)	633, 726, 815, 827, 1396, 1545
Bst4CI	ACNGT	3 cut(s)	166, 1757, 1784
Bst6I	CTCTTC	2 cut(s)	294, 1021
BstACI	GRCGYC	4 cut(s)	101, 135, 254, 260
BstC8I	GCNNGC	5 cut(s)	105, 225, 272, 401, 722
BstDEI	CTNAG	6 cut(s)	169, 360, 847, 921, 1092, 1717
BstDSI	CCRYGG	2 cut(s)	39, 1696
BstF5I	GGATG	5 cut(s)	12, 293, 838, 1433, 1876
BstFNI	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	5 cut(s)	46, 137, 272, 274, 467
BstMAI	GTCTC	1 cut(s)	172
BstMCI	CGRYCG	3 cut(s)	463, 1636, 1729
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 278, 1146, 1695, 1857
BstNI	CCWGG	6 cut(s)	633, 726, 815, 827, 1396, 1545
BstNSI	RCATGY	1 cut(s)	1064
BstSCI	CCNGG	9 cut(s)	631, 724, 813, 825, 898, 1394, 1448, 1543, 1630
BstSFI	CTRYAG	1 cut(s)	33
BstSLI	GKGCMC	1 cut(s)	194
BstUI	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
BstV1I	GCAGC	3 cut(s)	1234, 1284, 1872
BstV2I	GAAGAC	1 cut(s)	185
BstX2I	RGATCY	3 cut(s)	937, 1613, 1682
BstYI	RGATCY	3 cut(s)	937, 1613, 1682
Bsu15I	ATCGAT	2 cut(s)	1114, 1504
BsuI	GTATCC	2 cut(s)	495, 634
BsuRI	GGCC	6 cut(s)	107, 192, 238, 383, 578, 1335
BsuTUI	ATCGAT	2 cut(s)	1114, 1504
BtgI	CCRYGG	2 cut(s)	39, 1696
BtsCI	GGATG	5 cut(s)	12, 293, 838, 1433, 1876
BtsIMutI	CAGTG	1 cut(s)	1236
BtuMI	TCGCGA	1 cut(s)	344
BveI	ACCTGC	1 cut(s)	1190
Cac8I	GCNNGC	5 cut(s)	105, 225, 272, 401, 722
CaiI	CAGNNNCTG	1 cut(s)	632
CfoI	GCGC	5 cut(s)	46, 137, 272, 274, 467
Cfr10I	RCCGGY	2 cut(s)	103, 1326
Cfr42I	CCGCGG	1 cut(s)	1699
ClaI	ATCGAT	2 cut(s)	1114, 1504
CseI	GACGC	3 cut(s)	109, 243, 268
CsiI	ACCWGGT	2 cut(s)	631, 1543
Csp6I	GTAC	5 cut(s)	24, 199, 289, 663, 1265
CspAI	ACCGGT	1 cut(s)	1326
CspCI	CAANNNNNGTGG	2 cut(s)	1225, 1260
CviAII	CATG	7 cut(s)	542, 620, 1061, 1129, 1160, 1480, 1492
CviQI	GTAC	5 cut(s)	24, 199, 289, 663, 1265
DdeI	CTNAG	6 cut(s)	169, 360, 847, 921, 1092, 1717
DinI	GGCGCC	1 cut(s)	136
DraI	TTTAAA	1 cut(s)	526
EaeI	YGGCCR	2 cut(s)	381, 1333
Eam1104I	CTCTTC	2 cut(s)	294, 1021
EarI	CTCTTC	2 cut(s)	294, 1021
EciI	GGCGGA	1 cut(s)	8
Eco24I	GRGCYC	2 cut(s)	194, 760
Eco47I	GGWCC	6 cut(s)	48, 1043, 1191, 1532, 1541, 1622
Eco57I	CTGAAG	1 cut(s)	197
Eco88I	CYCGRG	2 cut(s)	95, 335
EcoO109I	RGGNCCY	2 cut(s)	191, 1191
EcoRI	GAATTC	1 cut(s)	614
EcoRII	CCWGG	6 cut(s)	631, 724, 813, 825, 1394, 1543
EcoT38I	GRGCYC	2 cut(s)	194, 760
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
Esp3I	CGTCTC	1 cut(s)	172
FaeI	CATG	7 cut(s)	545, 623, 1064, 1132, 1163, 1483, 1495
FaqI	GGGAC	3 cut(s)	112, 230, 1614
FatI	CATG	7 cut(s)	541, 619, 1060, 1128, 1159, 1479, 1491
FauI	CCCGC	1 cut(s)	441
FbaI	TGATCA	3 cut(s)	75, 802, 970
FblI	GTMKAC	2 cut(s)	257, 305
Fnu4HI	GCNGC	5 cut(s)	57, 145, 1248, 1298, 1861
FokI	GGATG	3 cut(s)	280, 845, 1440
FriOI	GRGCYC	2 cut(s)	194, 760
Fsp4HI	GCNGC	5 cut(s)	57, 145, 1248, 1298, 1861
FspBI	CTAG	6 cut(s)	153, 350, 1617, 1686, 1833, 1848
GlaI	GCGC	5 cut(s)	45, 136, 271, 273, 466
GluI	GCNGC	5 cut(s)	57, 145, 1248, 1298, 1861
GsaI	CCCAGC	1 cut(s)	1353
GsuI	CTGGAG	2 cut(s)	797, 1686
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	6 cut(s)	107, 192, 238, 383, 578, 1335
HapII	CCGG	7 cut(s)	104, 900, 1327, 1450, 1487, 1632, 1680
HgaI	GACGC	3 cut(s)	109, 243, 268
HhaI	GCGC	5 cut(s)	46, 137, 272, 274, 467
Hin1I	GRCGYC	4 cut(s)	101, 135, 254, 260
Hin1II	CATG	7 cut(s)	545, 623, 1064, 1132, 1163, 1483, 1495
Hin6I	GCGC	5 cut(s)	44, 135, 270, 272, 465
HinP1I	GCGC	5 cut(s)	44, 135, 270, 272, 465
HincII	GTYRAC	3 cut(s)	258, 306, 1462
HindII	GTYRAC	3 cut(s)	258, 306, 1462
HindIII	AAGCTT	2 cut(s)	914, 1138
HinfI	GANTC	9 cut(s)	302, 333, 556, 822, 965, 1016, 1506, 1592, 1655
HpaII	CCGG	7 cut(s)	104, 900, 1327, 1450, 1487, 1632, 1680
HphI	GGTGA	4 cut(s)	1294, 1316, 1601, 1664
Hpy166II	GTNNAC	6 cut(s)	258, 306, 1324, 1378, 1462, 1622
Hpy8I	GTNNAC	6 cut(s)	258, 306, 1324, 1378, 1462, 1622
Hpy99I	CGWCG	4 cut(s)	143, 259, 262, 326
HpyAV	CCTTC	6 cut(s)	79, 204, 306, 323, 604, 1356
HpyCH4III	ACNGT	3 cut(s)	166, 1757, 1784
HpyCH4IV	ACGT	1 cut(s)	868
HpyCH4V	TGCA	8 cut(s)	223, 442, 890, 1010, 1064, 1159, 1199, 1431
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 278, 1146, 1695, 1857
HpyF3I	CTNAG	6 cut(s)	169, 360, 847, 921, 1092, 1717
HpySE526I	ACGT	1 cut(s)	868
Hsp92I	GRCGYC	4 cut(s)	101, 135, 254, 260
Hsp92II	CATG	7 cut(s)	545, 623, 1064, 1132, 1163, 1483, 1495
HspAI	GCGC	5 cut(s)	44, 135, 270, 272, 465
KasI	GGCGCC	1 cut(s)	134
Kpn2I	TCCGGA	1 cut(s)	1679
KpnI	GGTACC	1 cut(s)	666
KroI	GCCGGC	1 cut(s)	103
KroNI	GCCGGC	1 cut(s)	105
Ksp22I	TGATCA	3 cut(s)	75, 802, 970
KspI	CCGCGG	1 cut(s)	1699
LguI	GCTCTTC	1 cut(s)	1021
LmnI	GCTCC	3 cut(s)	755, 816, 1441
Lsp1109I	GCAGC	3 cut(s)	1234, 1284, 1872
LweI	GCATC	7 cut(s)	798, 1019, 1040, 1096, 1136, 1418, 1473
MabI	ACCWGGT	2 cut(s)	631, 1543
MaeI	CTAG	6 cut(s)	153, 350, 1617, 1686, 1833, 1848
MaeII	ACGT	1 cut(s)	868
MaeIII	GTNAC	1 cut(s)	1712
MboII	GAAGA	5 cut(s)	190, 311, 1038, 1366, 1587
MflI	RGATCY	3 cut(s)	937, 1613, 1682
MhlI	GDGCHC	3 cut(s)	194, 691, 760
MlsI	TGGCCA	1 cut(s)	383
MluNI	TGGCCA	1 cut(s)	383
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	3 cut(s)	311, 327, 1010
MmeI	TCCRAC	1 cut(s)	884
Mox20I	TGGCCA	1 cut(s)	383
MroI	TCCGGA	1 cut(s)	1679
MroNI	GCCGGC	1 cut(s)	103
MroXI	GAANNNNTTC	1 cut(s)	614
MscI	TGGCCA	1 cut(s)	383
MseI	TTAA	1 cut(s)	525
MslI	CAYNNNNRTG	1 cut(s)	546
Msp20I	TGGCCA	1 cut(s)	383
MspA1I	CMGCKG	1 cut(s)	1698
MspI	CCGG	7 cut(s)	104, 900, 1327, 1450, 1487, 1632, 1680
MspR9I	CCNGG	9 cut(s)	633, 726, 815, 827, 900, 1396, 1450, 1545, 1632
MvaI	CCWGG	6 cut(s)	633, 726, 815, 827, 1396, 1545
MvnI	CGCG	6 cut(s)	46, 227, 272, 344, 465, 1698
MwoI	GCNNNNNNNGC	5 cut(s)	141, 278, 1146, 1695, 1857
NaeI	GCCGGC	1 cut(s)	105
NarI	GGCGCC	1 cut(s)	135
NciI	CCSGG	3 cut(s)	900, 1450, 1632
NgoMIV	GCCGGC	1 cut(s)	103
NlaIII	CATG	7 cut(s)	545, 623, 1064, 1132, 1163, 1483, 1495
NlaIV	GGNNCC	9 cut(s)	108, 136, 192, 579, 664, 757, 858, 1193, 1645
NruI	TCGCGA	1 cut(s)	344
NspI	RCATGY	1 cut(s)	1064
PaeR7I	CTCGAG	1 cut(s)	335
PauI	GCGCGC	1 cut(s)	270
PciSI	GCTCTTC	1 cut(s)	1021
PcsI	WCGNNNNNNNCGW	1 cut(s)	1633
PdiI	GCCGGC	1 cut(s)	105
PdmI	GAANNNNTTC	1 cut(s)	614
PfeI	GAWTC	6 cut(s)	556, 822, 965, 1506, 1592, 1655
Pfl23II	CGTACG	2 cut(s)	23, 198
PflFI	GACNNNGTC	1 cut(s)	1626
PfoI	TCCNGGA	1 cut(s)	825
PinAI	ACCGGT	1 cut(s)	1326
PkrI	GCNGC	5 cut(s)	58, 146, 1249, 1299, 1862
PleI	GAGTC	3 cut(s)	310, 327, 1010
PluTI	GGCGCC	1 cut(s)	138
PpsI	GAGTC	3 cut(s)	310, 327, 1010
PpuMI	RGGWCCY	1 cut(s)	1191
PsiI	TTATAA	1 cut(s)	1052
Psp5II	RGGWCCY	1 cut(s)	1191
Psp6I	CCWGG	6 cut(s)	631, 724, 813, 825, 1394, 1543
PspFI	CCCAGC	1 cut(s)	1349
PspGI	CCWGG	6 cut(s)	631, 724, 813, 825, 1394, 1543
PspLI	CGTACG	2 cut(s)	23, 198
PspN4I	GGNNCC	9 cut(s)	108, 136, 192, 579, 664, 757, 858, 1193, 1645
PspOMI	GGGCCC	1 cut(s)	190
PspPPI	RGGWCCY	1 cut(s)	1191
PstNI	CAGNNNCTG	1 cut(s)	632
PsuI	RGATCY	3 cut(s)	937, 1613, 1682
PsyI	GACNNNGTC	1 cut(s)	1626
PteI	GCGCGC	1 cut(s)	270
RruI	TCGCGA	1 cut(s)	344
RsaI	GTAC	5 cut(s)	25, 200, 290, 664, 1266
RsaNI	GTAC	5 cut(s)	24, 199, 289, 663, 1265
RseI	CAYNNNNRTG	1 cut(s)	546
SacII	CCGCGG	1 cut(s)	1699
SalI	GTCGAC	2 cut(s)	256, 304
SapI	GCTCTTC	1 cut(s)	1021
SaqAI	TTAA	1 cut(s)	525
SatI	GCNGC	5 cut(s)	57, 145, 1248, 1298, 1861
ScaI	AGTACT	1 cut(s)	290
SchI	GAGTC	3 cut(s)	311, 327, 1010
ScrFI	CCNGG	9 cut(s)	633, 726, 815, 827, 900, 1396, 1450, 1545, 1632
SduI	GDGCHC	3 cut(s)	194, 691, 760
SexAI	ACCWGGT	2 cut(s)	631, 1543
SfaNI	GCATC	7 cut(s)	798, 1019, 1040, 1096, 1136, 1418, 1473
SfcI	CTRYAG	1 cut(s)	33
SfoI	GGCGCC	1 cut(s)	136
Sfr274I	CTCGAG	1 cut(s)	335
Sfr303I	CCGCGG	1 cut(s)	1699
SgrBI	CCGCGG	1 cut(s)	1699
SgrDI	CGTCGACG	1 cut(s)	256
SinI	GGWCC	6 cut(s)	48, 1043, 1191, 1532, 1541, 1622
SlaI	CTCGAG	1 cut(s)	335
SmiMI	CAYNNNNRTG	1 cut(s)	546
SmlI	CTYRAG	1 cut(s)	335
SmoI	CTYRAG	1 cut(s)	335
SsiI	CCGC	7 cut(s)	19, 46, 57, 144, 448, 1696, 1698
SspDI	GGCGCC	1 cut(s)	134
SspI	AATATT	2 cut(s)	1225, 1317
SspMI	CTAG	6 cut(s)	153, 350, 1617, 1686, 1833, 1848
StyD4I	CCNGG	9 cut(s)	631, 724, 813, 825, 898, 1394, 1448, 1543, 1630
TaaI	ACNGT	3 cut(s)	166, 1757, 1784
TaiI	ACGT	1 cut(s)	871
TaqII	GACCGA	2 cut(s)	449, 1715
TatI	WGTACW	1 cut(s)	288
TauI	GCSGC	2 cut(s)	59, 147
TfiI	GAWTC	6 cut(s)	556, 822, 965, 1506, 1592, 1655
Tru1I	TTAA	1 cut(s)	525
Tru9I	TTAA	1 cut(s)	525
TscAI	CASTG	1 cut(s)	1243
TseI	GCWGC	3 cut(s)	1247, 1297, 1860
TspDTI	ATGAA	3 cut(s)	78, 342, 810
TspGWI	ACGGA	2 cut(s)	1038, 1454
TspRI	CASTG	1 cut(s)	1243
Tth111I	GACNNNGTC	1 cut(s)	1626
VpaK11BI	GGWCC	6 cut(s)	48, 1043, 1191, 1532, 1541, 1622
XapI	RAATTY	5 cut(s)	614, 763, 990, 1216, 1433
XceI	RCATGY	1 cut(s)	1064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1276
XhoI	CTCGAG	1 cut(s)	335
XmiI	GTMKAC	2 cut(s)	257, 305
XmnI	GAANNNNTTC	1 cut(s)	614
XspI	CTAG	6 cut(s)	153, 350, 1617, 1686, 1833, 1848
ZrmI	AGTACT	1 cut(s)	290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.